Rmu_ssc0000473.1_g000018

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000473.1
OG0020446
Physical Location & Seq
Reverse (-)
107253 .. 108977
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 789 bp
atggtcacagaagaagctcttccaacataccagacaatgcttaatactctagatggagttcgggatgagactggtgcaagcctcacctcctgggcaagatggaccagggcatggactgctgaagagaataggcatggagaccttctaaacaagtatctctatctctctggacgagtagatatgaaacaaatcgagaagacaattcagtatcttattggatcaggaatggatcctaaaacggagaacaatccctaccttggtttcatttatacttcatttcaagagagggctaccttcatctcccatgggaacacagccaggcaagcaaaagagcatggggatgtgaaattggctcagatatgtggcaccattgcctcggatgaaaaacgtcatgaaactgcctatacaaagattgtggagaagctctttgagatagacccagatggcactgtattggctttagctgacatgatgaagaaaaaaataacaatgcctgcccacttgatgtatgatggtcaggattataatcttttcgagaatttctcagctgttgcccagcggctggaagtttatacggccaaggattacgctgatattttggagtttctggttggtagatggaaaatagaggagttgactggtctttctggtgagggtcgtagagcacaagatttcgtatgtggattgccaccgagaattagaaggttggaggagagagctcagggaagagccaagaaatcatccagtgttccattcagctggctttttggtagagagataatcgtttaa

Protein Analysis

262

Amino Acids

29.89

Weight (kDa)

6.03

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 1 - 256 4.7e-130 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020446)

Species Orthologous Gene IDs
pyrus_communis pycom12g24230
rosa_multiflora Rmu_co8293057.1_g000001 Rmu_co8451205.1_g000001 Rmu_sc0016148.1_g000002 Rmu_ssc0000473.1_g000018
rosa_roxburghii Rroxscaffold_2G00120150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AccB1I GGYRCC 1 cut(s) 365
AccB7I CCANNNNNTGG 2 cut(s) 111, 562
AciI CCGC 1 cut(s) 559
AclWI GGATC 3 cut(s) 224, 226, 237
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 1 cut(s) 538
AcuI CTGAAG 1 cut(s) 141
AfiI CCNNNNNNNGG 3 cut(s) 111, 257, 562
AgsI TTSAA 1 cut(s) 281
AjnI CCWGG 3 cut(s) 89, 104, 317
AluBI AGCT 6 cut(s) 17, 424, 464, 548, 719, 759
AluI AGCT 6 cut(s) 17, 424, 464, 548, 719, 759
Alw21I GWGCWC 2 cut(s) 667, 721
Alw26I GTCTC 2 cut(s) 62, 132
AlwI GGATC 3 cut(s) 224, 226, 237
AlwNI CAGNNNCTG 1 cut(s) 562
AoxI GGCC 1 cut(s) 576
ApoI RAATTY 1 cut(s) 538
Asp700I GAANNNNTTC 1 cut(s) 18
AspS9I GGNCC 1 cut(s) 102
AsuHPI GGTGA 2 cut(s) 76, 662
AvaII GGWCC 1 cut(s) 102
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 1 cut(s) 365
BanII GRGCYC 1 cut(s) 721
BbsI GAAGAC 1 cut(s) 203
Bbv12I GWGCWC 2 cut(s) 667, 721
BccI CCATC 5 cut(s) 47, 93, 437, 506, 612
BceAI ACGGC 1 cut(s) 591
BciT130I CCWGG 3 cut(s) 91, 106, 319
BcoDI GTCTC 2 cut(s) 62, 132
BfaI CTAG 1 cut(s) 50
BisI GCNGC 1 cut(s) 560
BlsI GCNGC 1 cut(s) 561
Bme1390I CCNGG 3 cut(s) 91, 106, 319
Bme18I GGWCC 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 2 cut(s) 231, 367
BmrFI CCNGG 3 cut(s) 91, 106, 319
BpiI GAAGAC 1 cut(s) 203
BplI GAGNNNNNCTC 2 cut(s) 527, 559
Bpu10I CCTNAGC 1 cut(s) 720
BsaBI GATNNNNATC 1 cut(s) 525
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 6 cut(s) 90, 105, 256, 304, 375, 579
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 257, 562
Bse1I ACTGG 3 cut(s) 76, 643, 744
Bse3DI GCAATG 1 cut(s) 369
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 3 cut(s) 91, 106, 319
BseDI CCNNGG 6 cut(s) 90, 105, 256, 304, 375, 579
BseGI GGATG 4 cut(s) 70, 346, 385, 740
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 3 cut(s) 111, 257, 562
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 3 cut(s) 368, 558, 734
BseNI ACTGG 3 cut(s) 76, 643, 744
BseRI GAGGAG 2 cut(s) 644, 725
BseYI CCCAGC 1 cut(s) 555
BshFI GGCC 1 cut(s) 578
BshNI GGYRCC 1 cut(s) 365
BsiHKAI GWGCWC 2 cut(s) 667, 721
BslI CCNNNNNNNGG 3 cut(s) 111, 257, 562
BsmAI GTCTC 2 cut(s) 62, 132
BsnI GGCC 1 cut(s) 578
Bso31I GGTCTC 1 cut(s) 132
Bsp1286I GDGCHC 2 cut(s) 667, 721
Bsp143I GATC 2 cut(s) 218, 229
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 1 cut(s) 559
BspANI GGCC 1 cut(s) 578
BspCNI CTCAG 3 cut(s) 367, 557, 733
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 2 cut(s) 231, 367
BspPI GGATC 3 cut(s) 224, 226, 237
BspQI GCTCTTC 2 cut(s) 24, 721
BspT107I GGYRCC 1 cut(s) 365
BspTNI GGTCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 3 cut(s) 76, 643, 744
BssECI CCNNGG 6 cut(s) 90, 105, 256, 304, 375, 579
BssMI GATC 2 cut(s) 218, 229
BssT1I CCWWGG 3 cut(s) 256, 304, 579
Bst2UI CCWGG 3 cut(s) 91, 106, 319
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 3 cut(s) 24, 117, 721
BstAPI GCANNNNNTGC 1 cut(s) 116
BstC8I GCNNGC 4 cut(s) 79, 324, 495, 761
BstDEI CTNAG 3 cut(s) 354, 544, 720
BstDSI CCRYGG 1 cut(s) 304
BstF5I GGATG 4 cut(s) 70, 346, 385, 740
BstKTI GATC 2 cut(s) 221, 232
BstMAI GTCTC 2 cut(s) 62, 132
BstMBI GATC 2 cut(s) 218, 229
BstMWI GCNNNNNNNGC 2 cut(s) 116, 323
BstNI CCWGG 3 cut(s) 91, 106, 319
BstSCI CCNGG 3 cut(s) 89, 104, 317
BstV2I GAAGAC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 229
BstXI CCANNNNNNTGG 1 cut(s) 759
BstYI RGATCY 1 cut(s) 229
BsuRI GGCC 1 cut(s) 578
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 4 cut(s) 70, 346, 385, 740
BtsIMutI CAGTG 2 cut(s) 447, 751
Cac8I GCNNGC 4 cut(s) 79, 324, 495, 761
CaiI CAGNNNCTG 1 cut(s) 562
CciI TCATGA 1 cut(s) 391
Cfr13I GGNCC 1 cut(s) 102
CspCI CAANNNNNGTGG 2 cut(s) 396, 431
CviAII CATG 6 cut(s) 111, 134, 305, 335, 392, 469
DdeI CTNAG 3 cut(s) 354, 544, 720
DpnI GATC 2 cut(s) 220, 231
DpnII GATC 2 cut(s) 218, 229
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 3 cut(s) 24, 117, 721
EarI CTCTTC 3 cut(s) 24, 117, 721
Ecl136II GAGCTC 1 cut(s) 719
Eco130I CCWWGG 3 cut(s) 256, 304, 579
Eco24I GRGCYC 1 cut(s) 721
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 719
Eco57I CTGAAG 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 719
EcoRII CCWGG 3 cut(s) 89, 104, 317
EcoT14I CCWWGG 3 cut(s) 256, 304, 579
EcoT38I GRGCYC 1 cut(s) 721
ErhI CCWWGG 3 cut(s) 256, 304, 579
FaeI CATG 6 cut(s) 114, 137, 308, 338, 395, 472
FalI AAGNNNNNCTT 1 cut(s) 35
FatI CATG 6 cut(s) 110, 133, 304, 334, 391, 468
Fnu4HI GCNGC 1 cut(s) 560
FokI GGATG 4 cut(s) 77, 353, 392, 727
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 1 cut(s) 560
FspBI CTAG 1 cut(s) 50
GluI GCNGC 1 cut(s) 560
GsaI CCCAGC 1 cut(s) 559
HaeIII GGCC 1 cut(s) 578
Hin1II CATG 6 cut(s) 114, 137, 308, 338, 395, 472
HincII GTYRAC 1 cut(s) 636
HindII GTYRAC 1 cut(s) 636
HphI GGTGA 2 cut(s) 76, 662
Hpy166II GTNNAC 1 cut(s) 636
Hpy188I TCNGA 2 cut(s) 357, 379
Hpy188III TCNNGA 9 cut(s) 50, 62, 168, 193, 222, 281, 392, 518, 535
Hpy8I GTNNAC 1 cut(s) 636
HpyAV CCTTC 3 cut(s) 152, 304, 696
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 1 cut(s) 77
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 323
HpyF3I CTNAG 3 cut(s) 354, 544, 720
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 6 cut(s) 114, 137, 308, 338, 395, 472
Kzo9I GATC 2 cut(s) 218, 229
LguI GCTCTTC 2 cut(s) 24, 721
MaeI CTAG 1 cut(s) 50
MaeII ACGT 1 cut(s) 388
MaeIII GTNAC 1 cut(s) 4
MalI GATC 2 cut(s) 220, 231
MboI GATC 2 cut(s) 218, 229
MboII GAAGA 6 cut(s) 11, 23, 134, 208, 487, 738
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 2 cut(s) 667, 721
MluCI AATT 4 cut(s) 201, 347, 538, 696
MmeI TCCRAC 2 cut(s) 47, 687
MnlI CCTC 7 cut(s) 92, 97, 279, 385, 622, 646, 703
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 2 cut(s) 42, 787
MslI CAYNNNNRTG 1 cut(s) 339
MspA1I CMGCKG 3 cut(s) 548, 559, 759
MspR9I CCNGG 3 cut(s) 91, 106, 319
MvaI CCWGG 3 cut(s) 91, 106, 319
MwoI GCNNNNNNNGC 2 cut(s) 116, 323
NcoI CCATGG 1 cut(s) 304
NdeII GATC 2 cut(s) 218, 229
NlaIII CATG 6 cut(s) 114, 137, 308, 338, 395, 472
NlaIV GGNNCC 2 cut(s) 231, 367
NmuCI GTSAC 1 cut(s) 4
PagI TCATGA 1 cut(s) 391
PciSI GCTCTTC 2 cut(s) 24, 721
PdmI GAANNNNTTC 1 cut(s) 18
PflMI CCANNNNNTGG 2 cut(s) 111, 562
PkrI GCNGC 1 cut(s) 561
PsiI TTATAA 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 721
Psp6I CCWGG 3 cut(s) 89, 104, 317
PspFI CCCAGC 1 cut(s) 555
PspGI CCWGG 3 cut(s) 89, 104, 317
PspN4I GGNNCC 2 cut(s) 231, 367
PspPI GGNCC 1 cut(s) 102
PsrI GAACNNNNNNTAC 2 cut(s) 236, 268
PstNI CAGNNNCTG 1 cut(s) 562
PsuI RGATCY 1 cut(s) 229
PvuII CAGCTG 2 cut(s) 548, 759
RseI CAYNNNNRTG 1 cut(s) 339
SacI GAGCTC 1 cut(s) 721
SapI GCTCTTC 2 cut(s) 24, 721
SaqAI TTAA 2 cut(s) 42, 787
SatI GCNGC 1 cut(s) 560
Sau3AI GATC 2 cut(s) 218, 229
Sau96I GGNCC 1 cut(s) 102
ScrFI CCNGG 3 cut(s) 91, 106, 319
SduI GDGCHC 2 cut(s) 667, 721
SinI GGWCC 1 cut(s) 102
SmiMI CAYNNNNRTG 1 cut(s) 339
Sse9I AATT 4 cut(s) 201, 347, 538, 696
SsiI CCGC 1 cut(s) 559
SspMI CTAG 1 cut(s) 50
SstI GAGCTC 1 cut(s) 721
StyD4I CCNGG 3 cut(s) 89, 104, 317
StyI CCWWGG 3 cut(s) 256, 304, 579
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 1 cut(s) 391
TaqI TCGA 2 cut(s) 192, 534
TasI AATT 4 cut(s) 201, 347, 538, 696
TauI GCSGC 1 cut(s) 562
Tru1I TTAA 2 cut(s) 42, 787
Tru9I TTAA 2 cut(s) 42, 787
TscAI CASTG 2 cut(s) 454, 751
TseFI GTSAC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 7 cut(s) 197, 253, 264, 286, 396, 408, 488
TspGWI ACGGA 1 cut(s) 254
TspRI CASTG 2 cut(s) 454, 751
Van91I CCANNNNNTGG 2 cut(s) 111, 562
VpaK11BI GGWCC 1 cut(s) 102
XapI RAATTY 1 cut(s) 538
XbaI TCTAGA 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.