Rroxscaffold_1G00014510

Shikimate O-hydroxycinnamoyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0019015
Physical Location & Seq
Reverse (-)
17937178 .. 17937949
772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00014510.1

Sequence Viewer

Length: 684 bp
ATGGCAGTCAACGTTAGCATGAGAGAGTTAACGGTGGTGAGGCCAGCAGCGGAGACTCCTCAGCAGTCGTTGCAAGGGCAACAATTTCTTGGACATGCGCGTGTTAAAGGATGCGCTCAGCAAGGCCCTCGTGCCCTTTTACCCTTTGGCCGGCCGCCTACGGCAGAAAAGGACGAGGGAGAGAATGGTCGTATCGAAATCGATTGCAATGCAGAGGGGGCCTTGTTTGCTGTGGCCGATAGCAGCTTTTGCATCGATGACTTTGGCGATTTTGCGCCCACACCCGAATTCGGGAGAGGGCTCATCCCCACCGTTGACTATTCCGGTGGGATATCTTCTTTTCCCTTGTTGCTGGTTCAGATCACATACTTGAAATGCGGTGGAGTGGCACTTGGTCTTGGTGTGGACCATCGTGTATCAGATGGATTATCTACTTTACACTTCATAAATACATGGTCTGATATGGCTCGTGGTCTCGAGGTTGCAATTCGGCCAAGCATGGACAGGACGATGCTTCGTGCCCCGAGACCCCCTCGGCCTTTATCCGACTACAATCACCACATTGCATACCCTCCTCGGAATAAGCATGAGAGCACTGCAGCAACAACGGTTACAAGTTTTAGATTTATAAGGGGAGCAGATCAACGTCTTGAAAGCTATGTCAACCATGAACATAGCGGATGA

Protein Analysis

227

Amino Acids

24.77

Weight (kDa)

6.23

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 62 - 197 6.9e-32 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019015)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0066671
rosa_multiflora Rmu_sc0000605.1_g000046 Rmu_sc0013613.1_g000001
rosa_roxburghii Rroxscaffold_1G00014510
rosa_samantha Rh5BG454300 Rh5BG455200 Rh5CG476600 Rh5CG477300 Rh5DG468900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 629
AccII CGCG 1 cut(s) 100
AciI CCGC 4 cut(s) 50, 155, 378, 678
AclI AACGTT 1 cut(s) 12
AcoI YGGCCR 4 cut(s) 148, 152, 234, 491
AcsI RAATTY 1 cut(s) 287
AfiI CCNNNNNNNGG 3 cut(s) 150, 290, 291
AgsI TTSAA 2 cut(s) 373, 653
AluBI AGCT 2 cut(s) 246, 657
AluI AGCT 2 cut(s) 246, 657
Alw21I GWGCWC 1 cut(s) 596
Alw26I GTCTC 3 cut(s) 47, 479, 520
Ama87I CYCGRG 2 cut(s) 476, 523
AoxI GGCC 8 cut(s) 41, 124, 148, 152, 219, 234, 491, 536
ApeKI GCWGC 3 cut(s) 47, 243, 599
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 3 cut(s) 100, 116, 277
AspS9I GGNCC 3 cut(s) 125, 219, 406
AsuHPI GGTGA 2 cut(s) 49, 548
AvaI CYCGRG 2 cut(s) 476, 523
AvaII GGWCC 1 cut(s) 406
BaeGI GKGCMC 2 cut(s) 136, 523
BanII GRGCYC 1 cut(s) 303
BauI CACGAG 2 cut(s) 129, 468
Bbv12I GWGCWC 1 cut(s) 596
BbvCI CCTCAGC 1 cut(s) 60
BbvI GCAGC 3 cut(s) 59, 255, 611
BccI CCATC 2 cut(s) 416, 417
BceAI ACGGC 1 cut(s) 177
BcgI CGANNNNNNTGC 4 cut(s) 110, 144, 191, 225
BcoDI GTCTC 3 cut(s) 47, 479, 520
BfmI CTRYAG 1 cut(s) 597
BisI GCNGC 4 cut(s) 48, 155, 244, 600
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 4 cut(s) 49, 156, 245, 601
Bme18I GGWCC 1 cut(s) 406
BmeT110I CYCGRG 2 cut(s) 476, 523
BmgT120I GGNCC 3 cut(s) 125, 219, 406
BmiI GGNNCC 1 cut(s) 220
BmsI GCATC 3 cut(s) 101, 261, 501
BplI GAGNNNNNCTC 2 cut(s) 517, 549
Bpu10I CCTNAGC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 117
Bsa29I ATCGAT 2 cut(s) 201, 255
BsaI GGTCTC 2 cut(s) 479, 520
BsaJI CCNNGG 2 cut(s) 533, 575
BsaWI WCCGGW 1 cut(s) 323
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 290, 291
Bse118I RCCGGY 1 cut(s) 150
Bse3DI GCAATG 2 cut(s) 214, 561
BseCI ATCGAT 2 cut(s) 201, 255
BseDI CCNNGG 2 cut(s) 533, 575
BseGI GGATG 2 cut(s) 116, 303
BseLI CCNNNNNNNGG 3 cut(s) 150, 290, 291
BseMI GCAATG 2 cut(s) 214, 561
BseMII CTCAG 2 cut(s) 74, 131
BseRI GAGGAG 2 cut(s) 48, 564
BseSI GKGCMC 2 cut(s) 136, 523
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 3 cut(s) 59, 255, 611
Bsh1236I CGCG 1 cut(s) 100
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 8 cut(s) 43, 126, 150, 154, 221, 236, 493, 538
BshVI ATCGAT 2 cut(s) 201, 255
BsiEI CGRYCG 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 596
BsiHKCI CYCGRG 2 cut(s) 476, 523
BsiSI CCGG 2 cut(s) 151, 324
BslI CCNNNNNNNGG 3 cut(s) 150, 290, 291
BsmAI GTCTC 3 cut(s) 47, 479, 520
BsnI GGCC 8 cut(s) 43, 126, 150, 154, 221, 236, 493, 538
Bso31I GGTCTC 2 cut(s) 479, 520
BsoBI CYCGRG 2 cut(s) 476, 523
Bsp1286I GDGCHC 4 cut(s) 136, 303, 523, 596
Bsp143I GATC 2 cut(s) 360, 640
Bsp1720I GCTNAGC 1 cut(s) 117
BspACI CCGC 4 cut(s) 50, 155, 378, 678
BspANI GGCC 8 cut(s) 43, 126, 150, 154, 221, 236, 493, 538
BspCNI CTCAG 2 cut(s) 73, 130
BspDI ATCGAT 2 cut(s) 201, 255
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 1 cut(s) 220
BspMAI CTGCAG 1 cut(s) 601
BspTNI GGTCTC 2 cut(s) 479, 520
BsrDI GCAATG 2 cut(s) 214, 561
BsrFI RCCGGY 1 cut(s) 150
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 2 cut(s) 533, 575
BssMI GATC 2 cut(s) 360, 640
BssSI CACGAG 2 cut(s) 129, 468
Bst2BI CACGAG 2 cut(s) 129, 468
Bst4CI ACNGT 3 cut(s) 34, 313, 610
BstAPI GCANNNNNTGC 2 cut(s) 70, 249
BstC8I GCNNGC 2 cut(s) 45, 152
BstDEI CTNAG 2 cut(s) 60, 117
BstF5I GGATG 2 cut(s) 116, 303
BstFNI CGCG 1 cut(s) 100
BstHHI GCGC 3 cut(s) 100, 116, 277
BstKTI GATC 2 cut(s) 363, 643
BstMAI GTCTC 3 cut(s) 47, 479, 520
BstMBI GATC 2 cut(s) 360, 640
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 4 cut(s) 70, 218, 227, 249
BstNSI RCATGY 1 cut(s) 98
BstSFI CTRYAG 1 cut(s) 597
BstSLI GKGCMC 2 cut(s) 136, 523
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 3 cut(s) 59, 255, 611
BstZI CGGCCG 1 cut(s) 152
Bsu15I ATCGAT 2 cut(s) 201, 255
BsuRI GGCC 8 cut(s) 43, 126, 150, 154, 221, 236, 493, 538
BsuTUI ATCGAT 2 cut(s) 201, 255
BtsCI GGATG 2 cut(s) 116, 303
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 1 cut(s) 594
Cac8I GCNNGC 2 cut(s) 45, 152
CfoI GCGC 3 cut(s) 100, 116, 277
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 3 cut(s) 125, 219, 406
ClaI ATCGAT 2 cut(s) 201, 255
CviAII CATG 6 cut(s) 19, 95, 453, 499, 587, 668
DdeI CTNAG 2 cut(s) 60, 117
DpnI GATC 2 cut(s) 362, 642
DpnII GATC 2 cut(s) 360, 640
EaeI YGGCCR 4 cut(s) 148, 152, 234, 491
EagI CGGCCG 1 cut(s) 152
EclXI CGGCCG 1 cut(s) 152
Eco24I GRGCYC 1 cut(s) 303
Eco31I GGTCTC 2 cut(s) 479, 520
Eco32I GATATC 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 406
Eco52I CGGCCG 1 cut(s) 152
Eco88I CYCGRG 2 cut(s) 476, 523
EcoO109I RGGNCCY 2 cut(s) 125, 219
EcoRI GAATTC 1 cut(s) 287
EcoRV GATATC 1 cut(s) 333
EcoT38I GRGCYC 1 cut(s) 303
FaeI CATG 6 cut(s) 22, 98, 456, 502, 590, 671
FatI CATG 6 cut(s) 18, 94, 452, 498, 586, 667
Fnu4HI GCNGC 4 cut(s) 48, 155, 244, 600
FokI GGATG 2 cut(s) 123, 290
FriOI GRGCYC 1 cut(s) 303
FseI GGCCGGCC 1 cut(s) 154
Fsp4HI GCNGC 4 cut(s) 48, 155, 244, 600
GlaI GCGC 3 cut(s) 99, 115, 276
GluI GCNGC 4 cut(s) 48, 155, 244, 600
HaeIII GGCC 8 cut(s) 43, 126, 150, 154, 221, 236, 493, 538
HapII CCGG 2 cut(s) 151, 324
HhaI GCGC 3 cut(s) 100, 116, 277
Hin1II CATG 6 cut(s) 22, 98, 456, 502, 590, 671
Hin6I GCGC 3 cut(s) 98, 114, 275
HinP1I GCGC 3 cut(s) 98, 114, 275
HincII GTYRAC 4 cut(s) 10, 30, 316, 664
HindII GTYRAC 4 cut(s) 10, 30, 316, 664
HinfI GANTC 1 cut(s) 55
HpaI GTTAAC 1 cut(s) 30
HpaII CCGG 2 cut(s) 151, 324
HphI GGTGA 2 cut(s) 49, 548
Hpy166II GTNNAC 5 cut(s) 10, 30, 316, 406, 664
Hpy188I TCNGA 5 cut(s) 360, 421, 460, 547, 579
Hpy188III TCNNGA 3 cut(s) 292, 476, 650
Hpy8I GTNNAC 5 cut(s) 10, 30, 316, 406, 664
HpyCH4III ACNGT 3 cut(s) 34, 313, 610
HpyCH4IV ACGT 2 cut(s) 12, 646
HpyCH4V TGCA 7 cut(s) 73, 207, 212, 252, 485, 566, 599
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 218, 227, 249
HpyF3I CTNAG 2 cut(s) 60, 117
HpySE526I ACGT 2 cut(s) 12, 646
Hsp92II CATG 6 cut(s) 22, 98, 456, 502, 590, 671
HspAI GCGC 3 cut(s) 98, 114, 275
KroI GCCGGC 1 cut(s) 150
KroNI GCCGGC 1 cut(s) 152
KspAI GTTAAC 1 cut(s) 30
Kzo9I GATC 2 cut(s) 360, 640
LmnI GCTCC 1 cut(s) 635
LpnPI CCDG 5 cut(s) 57, 164, 337, 338, 490
Lsp1109I GCAGC 3 cut(s) 59, 255, 611
LweI GCATC 3 cut(s) 101, 261, 501
MaeII ACGT 2 cut(s) 12, 646
MaeIII GTNAC 1 cut(s) 610
MalI GATC 2 cut(s) 362, 642
MboI GATC 2 cut(s) 360, 640
MboII GAAGA 1 cut(s) 327
MhlI GDGCHC 4 cut(s) 136, 303, 523, 596
MluCI AATT 3 cut(s) 83, 287, 486
MlyI GAGTC 1 cut(s) 49
MmeI TCCRAC 1 cut(s) 570
MroNI GCCGGC 1 cut(s) 150
MseI TTAA 2 cut(s) 29, 105
MslI CAYNNNNRTG 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 2 cut(s) 151, 324
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 4 cut(s) 70, 218, 227, 249
NaeI GCCGGC 1 cut(s) 152
NdeII GATC 2 cut(s) 360, 640
NgoMIV GCCGGC 1 cut(s) 150
NlaIII CATG 6 cut(s) 22, 98, 456, 502, 590, 671
NlaIV GGNNCC 1 cut(s) 220
NmeAIII GCCGAG 1 cut(s) 514
NspI RCATGY 1 cut(s) 98
PaeR7I CTCGAG 1 cut(s) 476
PdiI GCCGGC 1 cut(s) 152
PkrI GCNGC 4 cut(s) 49, 156, 245, 601
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
PsiI TTATAA 1 cut(s) 629
Psp1406I AACGTT 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 220
PspPI GGNCC 3 cut(s) 125, 219, 406
PstI CTGCAG 1 cut(s) 601
RigI GGCCGGCC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 99
SaqAI TTAA 2 cut(s) 29, 105
SatI GCNGC 4 cut(s) 48, 155, 244, 600
Sau3AI GATC 2 cut(s) 360, 640
Sau96I GGNCC 3 cut(s) 125, 219, 406
SchI GAGTC 1 cut(s) 49
SduI GDGCHC 4 cut(s) 136, 303, 523, 596
SetI ASST 5 cut(s) 15, 248, 483, 649, 659
SfaNI GCATC 3 cut(s) 101, 261, 501
SfcI CTRYAG 1 cut(s) 597
Sfr274I CTCGAG 1 cut(s) 476
SinI GGWCC 1 cut(s) 406
SlaI CTCGAG 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 3 cut(s) 83, 287, 486
SsiI CCGC 4 cut(s) 50, 155, 378, 678
TaaI ACNGT 3 cut(s) 34, 313, 610
TaiI ACGT 2 cut(s) 15, 649
TaqI TCGA 4 cut(s) 195, 201, 255, 477
TasI AATT 3 cut(s) 83, 287, 486
TauI GCSGC 1 cut(s) 157
Tru1I TTAA 2 cut(s) 29, 105
Tru9I TTAA 2 cut(s) 29, 105
TscAI CASTG 1 cut(s) 601
TseI GCWGC 3 cut(s) 47, 243, 599
TspDTI ATGAA 2 cut(s) 433, 684
TspRI CASTG 1 cut(s) 601
VpaK11BI GGWCC 1 cut(s) 406
XapI RAATTY 1 cut(s) 287
XceI RCATGY 1 cut(s) 98
XhoI CTCGAG 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.