Rroxscaffold_1G00021560

Vacuolar fusion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013229
Physical Location & Seq
Forward (+)
26474906 .. 26482768
7863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00021560.1

Sequence Viewer

Length: 1356 bp
ATGACCTCCGCGTCGAGCTCCGGCGACGAAACCTCCGACCCGAAGCCCAACCCGATTACAAAGCCCCCGGAGGACCAATTGGCCGCCCTCGCGTTATCCGAGACCGATAGCCAAGCAAACGGCGTCGCATGTGAATCGCCGCGCGGGAACAATCACAATGAGATCGAGAACGAAGAGGAGGCGGCGGCGCTGACCAATTCGGCTCCGTCTGGTTCGGCTGAGGTGGTGGAATCGAGCGGTGGTGGTATTATGTGGGCGAGGAGCAATTCGGAGCTGGAGGTCGACGGGGCGGAGAGCCCTAGCAGCAGCGGCTATGCCGGCGGCAGAGGGAGTAGTAGTGCCAGTAGTGATGGTTCCGGGATTGATGAGATTGCGGAAGTTAGTGATGTTGAGGTTGTTGATGGTGTTTCGGATTCACATGCGCCGTGGGTGCCCGGAAAACGGCATGTTGATGAGGATGATGCTTCCATATCATGGAGAAAAAGGAAGAAGCACTTTTTCATACTAAGTAACTCTGGCAAACCAATATATTCCAGACATGGAGATGAACACAAGCTAGCAGGATTTTCAGCAACATTACAAGCCATAATTTCCTTTGTTGAGAATGGTGGAGATCGTGTCAAATTGGTTAGGGCTGGGAAGCATCAGGTGGTTTTTCTTGTCAAGGGACCAATTTACCTGGTATGCATCAGCTGCACAGAAGAGCCTTATGAGTCTTTAAGAATGCAATTGGAACTTATATACGGTCAGATGCTGCTTATTCTTACGAAGTCAGTAAATAGATGTTTTGAAAAGAATCCGAAGTTTGATATGACACCTTTGCTGGGAGGAACAGATATTGTCTTCTCTTCCCTCATCCATTCTTTCAGTTGGAATCCTGCCACATTTCTTCATGCATATACTTGTCTTCCCCTTGCTTATGCGACAAGGCAAGCTGCAGGTGCCATATTGCAAGATGTTGCTGATTCAGGTGTTCTCTTTGCAATATTGATGTGCAAACACAAGGTTATCAGTCTTGTTGGTGCTCAGAAAGCGTCTCTTCATCCTGATGACATGCTATTGCTATCCAACTTTGTTATGGCATCAGAATCATTTAGGACATCTGAATCTTTCTCACCAATTTGCTTGCCAAGATATAATGCCATGGCATTTTTGTATGCTTATGTCCATTTTTTTGATGCTGACACATACTTGATGTTGCTTACTACTAGTTCAGAAGCCTTCTATCATCTCAAAGATTGCAGGGGTGCTTCACGGGAAACTTTCATACATCCTATTGCCTTGCAAATTAATGCTCACCTCCTCCCTATAAATTTTTGTGACCTTATAGGATTCGTATTGAAATGGTCCTTCTGA

Protein Analysis

451

Amino Acids

48.98

Weight (kDa)

5.19

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fuz_longin_1 PF19036 164 - 284 3.4e-30 First Longin domain of FUZ, MON1 and HPS1
Fuz_longin_2 PF19037 324 - 415 1.6e-21 Second Longin domain of FUZ, MON1 and HPS1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013229)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28390
fragaria_vesca FvH4_3g32210 FvH4_3g32210 FvH4_3g32210
malus_domestica MD03G1118200.v1.1 MD11G1136600.v1.1
prunus_persica Prupe.6G102100_v2.0.a1 Prupe.6G102100_v2.0.a1
pyrus_communis pycom11g11310
rosa_chinensis RchiOBHm_Chr5g0058821
rosa_laevigata RLG00000035254
rosa_roxburghii Rroxscaffold_1G00021560
rosa_rugosa Rorug05G0319400
rosa_samantha Rh5AG384200 Rh5BG396100 Rh5CG420000 Rh5DG411900
rosa_wichuraiana Rw5G036200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 929
AasI GACNNNNNNGTC 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 929
AccB1I GGYRCC 2 cut(s) 430, 941
AccB7I CCANNNNNTGG 1 cut(s) 474
AccBSI CCGCTC 1 cut(s) 237
AccI GTMKAC 1 cut(s) 282
AccII CGCG 4 cut(s) 11, 92, 142, 144
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 1312
AcyI GRCGYC 1 cut(s) 123
AfiI CCNNNNNNNGG 3 cut(s) 441, 474, 824
AgsI TTSAA 2 cut(s) 791, 1342
AhlI ACTAGT 1 cut(s) 1208
AjnI CCWGG 1 cut(s) 678
AluBI AGCT 5 cut(s) 18, 274, 556, 693, 935
AluI AGCT 5 cut(s) 18, 274, 556, 693, 935
Alw21I GWGCWC 2 cut(s) 20, 1027
Alw26I GTCTC 2 cut(s) 95, 1041
AlwNI CAGNNNCTG 1 cut(s) 754
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 5 cut(s) 303, 306, 693, 754, 935
ApoI RAATTY 1 cut(s) 1312
AseI ATTAAT 1 cut(s) 1290
AspLEI GCGC 3 cut(s) 144, 190, 424
AspS9I GGNCC 3 cut(s) 73, 668, 1347
AsuC2I CCSGG 3 cut(s) 68, 358, 435
AsuHPI GGTGA 2 cut(s) 1107, 1289
AsuNHI GCTAGC 1 cut(s) 556
AvaII GGWCC 3 cut(s) 73, 668, 1347
BaeGI GKGCMC 1 cut(s) 435
BanI GGYRCC 2 cut(s) 430, 941
BanII GRGCYC 2 cut(s) 20, 299
BbsI GAAGAC 2 cut(s) 835, 899
Bbv12I GWGCWC 2 cut(s) 20, 1027
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 5 cut(s) 315, 318, 680, 741, 922
BccI CCATC 2 cut(s) 344, 395
BceAI ACGGC 3 cut(s) 136, 409, 458
BcgI CGANNNNNNTGC 2 cut(s) 117, 151
BciT130I CCWGG 1 cut(s) 680
BcnI CCSGG 3 cut(s) 68, 358, 435
BcoDI GTCTC 2 cut(s) 95, 1041
BcuI ACTAGT 1 cut(s) 1208
BfaI CTAG 3 cut(s) 300, 557, 1209
BfmI CTRYAG 1 cut(s) 936
BfoI RGCGCY 1 cut(s) 191
BfuAI ACCTGC 1 cut(s) 929
Bme1390I CCNGG 4 cut(s) 68, 358, 435, 680
Bme18I GGWCC 3 cut(s) 73, 668, 1347
BmgT120I GGNCC 3 cut(s) 73, 668, 1347
BmiI GGNNCC 5 cut(s) 204, 355, 432, 669, 943
BmrFI CCNGG 4 cut(s) 68, 358, 435, 680
BmsI GCATC 6 cut(s) 451, 652, 696, 741, 1091, 1168
BmtI GCTAGC 1 cut(s) 560
BpiI GAAGAC 2 cut(s) 835, 899
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 1 cut(s) 219
BpuMI CCSGG 3 cut(s) 68, 358, 435
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 66, 425, 1143
BsaXI ACNNNNNCTCC 6 cut(s) 17, 47, 322, 352, 603, 633
Bsc4I CCNNNNNNNGG 3 cut(s) 441, 474, 824
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 680
BseDI CCNNGG 3 cut(s) 66, 425, 1143
BseGI GGATG 4 cut(s) 463, 855, 1042, 1270
BseLI CCNNNNNNNGG 3 cut(s) 441, 474, 824
BseMII CTCAG 2 cut(s) 210, 1040
BseNI ACTGG 1 cut(s) 342
BseRI GAGGAG 3 cut(s) 191, 274, 1292
BseSI GKGCMC 1 cut(s) 435
BseXI GCAGC 5 cut(s) 315, 318, 680, 741, 922
BseYI CCCAGC 2 cut(s) 635, 823
BsgI GTGCAG 1 cut(s) 679
Bsh1236I CGCG 4 cut(s) 11, 92, 142, 144
BshFI GGCC 1 cut(s) 83
BshNI GGYRCC 2 cut(s) 430, 941
BsiHKAI GWGCWC 2 cut(s) 20, 1027
BsiSI CCGG 5 cut(s) 21, 68, 318, 357, 435
BslFI GGGAC 1 cut(s) 681
BslI CCNNNNNNNGG 3 cut(s) 441, 474, 824
BsmAI GTCTC 2 cut(s) 95, 1041
BsmBI CGTCTC 1 cut(s) 1041
BsmFI GGGAC 1 cut(s) 681
BsmI GAATGC 1 cut(s) 729
BsnI GGCC 1 cut(s) 83
Bso31I GGTCTC 1 cut(s) 95
Bsp1286I GDGCHC 4 cut(s) 20, 299, 435, 1027
Bsp143I GATC 2 cut(s) 162, 613
Bsp19I CCATGG 1 cut(s) 1143
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 2 cut(s) 211, 1039
BspFNI CGCG 4 cut(s) 11, 92, 142, 144
BspLI GGNNCC 5 cut(s) 204, 355, 432, 669, 943
BspMAI CTGCAG 1 cut(s) 940
BspMI ACCTGC 1 cut(s) 929
BspOI GCTAGC 1 cut(s) 560
BspQI GCTCTTC 1 cut(s) 696
BspT107I GGYRCC 2 cut(s) 430, 941
BspTNI GGTCTC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 1 cut(s) 342
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 3 cut(s) 66, 425, 1143
BssMI GATC 2 cut(s) 162, 613
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 1143
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 1 cut(s) 746
Bst6I CTCTTC 4 cut(s) 168, 696, 853, 1044
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 693
BstC8I GCNNGC 4 cut(s) 319, 558, 933, 1127
BstDEI CTNAG 3 cut(s) 219, 506, 1026
BstDSI CCRYGG 2 cut(s) 425, 1143
BstF5I GGATG 4 cut(s) 463, 855, 1042, 1270
BstFNI CGCG 4 cut(s) 11, 92, 142, 144
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 3 cut(s) 144, 190, 424
BstKTI GATC 2 cut(s) 165, 616
BstMAI GTCTC 2 cut(s) 95, 1041
BstMBI GATC 2 cut(s) 162, 613
BstMWI GCNNNNNNNGC 8 cut(s) 89, 303, 309, 318, 430, 693, 941, 1031
BstNI CCWGG 1 cut(s) 680
BstNSI RCATGY 4 cut(s) 132, 422, 449, 1057
BstSCI CCNGG 4 cut(s) 66, 356, 433, 678
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 1 cut(s) 435
BstUI CGCG 4 cut(s) 11, 92, 142, 144
BstV1I GCAGC 5 cut(s) 315, 318, 680, 741, 922
BstV2I GAAGAC 2 cut(s) 835, 899
BsuRI GGCC 1 cut(s) 83
BtgI CCRYGG 2 cut(s) 425, 1143
BtsCI GGATG 4 cut(s) 463, 855, 1042, 1270
BveI ACCTGC 1 cut(s) 929
Cac8I GCNNGC 4 cut(s) 319, 558, 933, 1127
CaiI CAGNNNCTG 1 cut(s) 754
CfoI GCGC 3 cut(s) 144, 190, 424
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 3 cut(s) 73, 668, 1347
CseI GACGC 2 cut(s) 112, 1023
CsiI ACCWGGT 1 cut(s) 678
CviAII CATG 8 cut(s) 129, 419, 446, 474, 539, 893, 1054, 1144
DdeI CTNAG 3 cut(s) 219, 506, 1026
DpnI GATC 2 cut(s) 164, 615
DpnII GATC 2 cut(s) 162, 613
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 4 cut(s) 168, 696, 853, 1044
EarI CTCTTC 4 cut(s) 168, 696, 853, 1044
EciI GGCGGA 1 cut(s) 305
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 1143
Eco24I GRGCYC 2 cut(s) 20, 299
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 3 cut(s) 73, 668, 1347
Eco53kI GAGCTC 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 678
EcoT14I CCWWGG 1 cut(s) 1143
EcoT22I ATGCAT 2 cut(s) 689, 898
EcoT38I GRGCYC 2 cut(s) 20, 299
ErhI CCWWGG 1 cut(s) 1143
Esp3I CGTCTC 1 cut(s) 1041
FaeI CATG 8 cut(s) 132, 422, 449, 477, 542, 896, 1057, 1147
FalI AAGNNNNNCTT 4 cut(s) 479, 511, 1023, 1055
FaqI GGGAC 1 cut(s) 681
FatI CATG 8 cut(s) 128, 418, 445, 473, 538, 892, 1053, 1143
FauI CCCGC 1 cut(s) 137
FblI GTMKAC 1 cut(s) 282
FokI GGATG 4 cut(s) 470, 842, 1029, 1257
FriOI GRGCYC 2 cut(s) 20, 299
FspBI CTAG 3 cut(s) 300, 557, 1209
GlaI GCGC 3 cut(s) 143, 189, 423
GsaI CCCAGC 2 cut(s) 639, 827
GsuI CTGGAG 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 1 cut(s) 83
HapII CCGG 5 cut(s) 21, 68, 318, 357, 435
HgaI GACGC 2 cut(s) 112, 1023
HhaI GCGC 3 cut(s) 144, 190, 424
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 8 cut(s) 132, 422, 449, 477, 542, 896, 1057, 1147
Hin6I GCGC 3 cut(s) 142, 188, 422
HinP1I GCGC 3 cut(s) 142, 188, 422
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HpaII CCGG 5 cut(s) 21, 68, 318, 357, 435
HphI GGTGA 2 cut(s) 1107, 1289
Hpy166II GTNNAC 1 cut(s) 283
Hpy188III TCNNGA 3 cut(s) 166, 534, 1046
Hpy8I GTNNAC 1 cut(s) 283
Hpy99I CGWCG 4 cut(s) 16, 29, 128, 287
HpyAV CCTTC 1 cut(s) 1231
HpyCH4III ACNGT 1 cut(s) 746
HpyF10VI GCNNNNNNNGC 8 cut(s) 89, 303, 309, 318, 430, 693, 941, 1031
HpyF3I CTNAG 3 cut(s) 219, 506, 1026
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 8 cut(s) 132, 422, 449, 477, 542, 896, 1057, 1147
HspAI GCGC 3 cut(s) 142, 188, 422
KroI GCCGGC 1 cut(s) 317
KroNI GCCGGC 1 cut(s) 319
Kzo9I GATC 2 cut(s) 162, 613
LguI GCTCTTC 1 cut(s) 696
LmnI GCTCC 4 cut(s) 23, 208, 261, 271
Lsp1109I GCAGC 5 cut(s) 315, 318, 680, 741, 922
LweI GCATC 6 cut(s) 451, 652, 696, 741, 1091, 1168
MabI ACCWGGT 1 cut(s) 678
MaeI CTAG 3 cut(s) 300, 557, 1209
MaeIII GTNAC 2 cut(s) 509, 1319
MalI GATC 2 cut(s) 164, 615
MbiI CCGCTC 1 cut(s) 237
MboI GATC 2 cut(s) 162, 613
MboII GAAGA 8 cut(s) 185, 499, 713, 835, 840, 881, 899, 1031
MfeI CAATTG 2 cut(s) 77, 728
MhlI GDGCHC 4 cut(s) 20, 299, 435, 1027
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 3 cut(s) 60, 851, 1092
Mph1103I ATGCAT 2 cut(s) 689, 898
MroNI GCCGGC 1 cut(s) 317
MseI TTAA 2 cut(s) 719, 1290
MslI CAYNNNNRTG 3 cut(s) 450, 543, 1047
MspA1I CMGCKG 2 cut(s) 309, 693
MspI CCGG 5 cut(s) 21, 68, 318, 357, 435
MspR9I CCNGG 4 cut(s) 68, 358, 435, 680
MunI CAATTG 2 cut(s) 77, 728
Mva1269I GAATGC 1 cut(s) 729
MvaI CCWGG 1 cut(s) 680
MvnI CGCG 4 cut(s) 11, 92, 142, 144
MwoI GCNNNNNNNGC 8 cut(s) 89, 303, 309, 318, 430, 693, 941, 1031
NaeI GCCGGC 1 cut(s) 319
NciI CCSGG 3 cut(s) 68, 358, 435
NcoI CCATGG 1 cut(s) 1143
NdeII GATC 2 cut(s) 162, 613
NgoMIV GCCGGC 1 cut(s) 317
NheI GCTAGC 1 cut(s) 556
NlaIII CATG 8 cut(s) 132, 422, 449, 477, 542, 896, 1057, 1147
NlaIV GGNNCC 5 cut(s) 204, 355, 432, 669, 943
NmuCI GTSAC 1 cut(s) 1319
NsiI ATGCAT 2 cut(s) 689, 898
NspI RCATGY 4 cut(s) 132, 422, 449, 1057
PaqCI CACCTGC 1 cut(s) 929
PciSI GCTCTTC 1 cut(s) 696
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 96
PctI GAATGC 1 cut(s) 729
PdiI GCCGGC 1 cut(s) 319
PfeI GAWTC 9 cut(s) 134, 230, 413, 796, 874, 965, 1088, 1106, 1332
PflMI CCANNNNNTGG 1 cut(s) 474
PfoI TCCNGGA 1 cut(s) 356
PleI GAGTC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 721
PshBI ATTAAT 1 cut(s) 1290
Psp124BI GAGCTC 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 678
PspFI CCCAGC 2 cut(s) 635, 823
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 5 cut(s) 204, 355, 432, 669, 943
PspPI GGNCC 3 cut(s) 73, 668, 1347
PsrI GAACNNNNNNTAC 2 cut(s) 337, 369
PstI CTGCAG 1 cut(s) 940
PstNI CAGNNNCTG 1 cut(s) 754
PvuII CAGCTG 1 cut(s) 693
RseI CAYNNNNRTG 3 cut(s) 450, 543, 1047
SacI GAGCTC 1 cut(s) 20
SalI GTCGAC 1 cut(s) 281
SapI GCTCTTC 1 cut(s) 696
SaqAI TTAA 2 cut(s) 719, 1290
Sau3AI GATC 2 cut(s) 162, 613
Sau96I GGNCC 3 cut(s) 73, 668, 1347
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 4 cut(s) 68, 358, 435, 680
SduI GDGCHC 4 cut(s) 20, 299, 435, 1027
SexAI ACCWGGT 1 cut(s) 678
SfaNI GCATC 6 cut(s) 451, 652, 696, 741, 1091, 1168
SfcI CTRYAG 1 cut(s) 936
SinI GGWCC 3 cut(s) 73, 668, 1347
SmiMI CAYNNNNRTG 3 cut(s) 450, 543, 1047
SpeI ACTAGT 1 cut(s) 1208
SspI AATATT 1 cut(s) 987
SspMI CTAG 3 cut(s) 300, 557, 1209
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 4 cut(s) 66, 356, 433, 678
StyI CCWWGG 1 cut(s) 1143
TaaI ACNGT 1 cut(s) 746
TaqI TCGA 4 cut(s) 14, 165, 233, 282
TaqII GACCGA 1 cut(s) 119
TauI GCSGC 6 cut(s) 86, 142, 185, 188, 312, 324
TfiI GAWTC 9 cut(s) 134, 230, 413, 796, 874, 965, 1088, 1106, 1332
Tru1I TTAA 2 cut(s) 719, 1290
Tru9I TTAA 2 cut(s) 719, 1290
TseFI GTSAC 1 cut(s) 1319
TseI GCWGC 5 cut(s) 303, 306, 693, 754, 935
Tsp45I GTSAC 1 cut(s) 1319
TspDTI ATGAA 5 cut(s) 490, 561, 881, 1031, 1255
TspGWI ACGGA 1 cut(s) 195
Van91I CCANNNNNTGG 1 cut(s) 474
VpaK11BI GGWCC 3 cut(s) 73, 668, 1347
VspI ATTAAT 1 cut(s) 1290
XapI RAATTY 1 cut(s) 1312
XceI RCATGY 4 cut(s) 132, 422, 449, 1057
XcmI CCANNNNNNNNNTGG 1 cut(s) 1075
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 3 cut(s) 300, 557, 1209
Zsp2I ATGCAT 2 cut(s) 689, 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.