Rh5DG411900

Vacuolar fusion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013229
Physical Location & Seq
Reverse (-)
65409332 .. 65417516
8185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG411900.1

Sequence Viewer

Length: 1647 bp
ATGACCTCCGCGTCGAGCTCCGGCGACGAAACCTCCGACCCGAAGCCCAACCCGATTACAAAGCCCCCGGAGGACCAATTGGCCGCCCTCGCGTTATCCGACACCGATAGCCAAGCAAACGGCGTCGCAAATGAATCGCCGCGCGGGAACAATCACAATGAGATCGAGAACGAAGAGGAGGCGGCGGCGCTGACCAATTCGGCTCCGTCCGGTTCGTCTGAGGTGGTGGAATCGAGCGGTGGTGGTATTCTGTGGGCGAGGAGCAATTCGGAGCTGGAGGCCGACGGGCCGGAGAGCCCTAGCAGCAGCGGCTATGCCGGCGGCAGAGGGACTAGTAGTGCCAGTAGTGATGGTTCCGGGATCGATGAGATTGCGGAAGTTAGTGATGTTGAGGTTGTTGATGGTGTTTCGGATTCACAAGCGCCGTGGGTGCCCGGAAAACGGCATGTTGATGAGGATGATGCTTCCATATCATGGAGAAAAAGGAAGAAGCACTTTTTCATACTTAGTAACTCTGGCAAACCAATATATTCCAGACATGGAGATGAACACAAACTAGCAGGATTTTCAGCAACATTACAAGCCATAATTTCCTTTGTTGAGAATGGTGGAGATCGTGTCAAATTGGTTAGGGCTGGGAAGCATCAGGTGGTTTTTCTTGTCAAGGGACCAATTTACCTGGTATGCATCAGCTGCACAGAAGAGCCTTATGAGTCTTTAAGAATGCAATTGGAACTTATATACGGTCAGATGCTGCTTATTCTTACAAAGTCGGTAAATAGATGTTTTGAGAAGAATCCGAAGTTTGATATGACACCTTTGCTGGGAGGAACAGATAGTGTCTTCTCTTCCCTCATCCATTCTTTCAGTTGGAATCCTGCCACATTTCTTCATGCATATACTTGTCTTCCCCTTGCTTATGCGACAAGGCAAGCTGCAGGTGCCATATTGCAAGATGTTGCTGATTCAGGTGTCCTCTTTGCAATATTGATGTGCAAACACAAGGTTATCAGTCTTGTTGGTGCTCAGAAAGCATCTCTTCATCCTGATGACATGCTATTGCTATCCAACTTTGTTATGGCATCAGAATCATTTAGGACATCTGAATCTTTCTCACCAATTTGCTTGCCAAGATATAATGCCATGGCATTTTTGTATGCTTATGTCCATTTTTTCGATGCTGACACATACTTGATGTTGCTTACTACTAGTTCAGAAGCCTTCTATCATCTTAAAGATTGCAGGATTCGTATTGAAATGGTCCTTCTGAAGTCGAGTGTCCTCAGTGAGATTCAGAGATCCATGCTAGATGGTGGAATGCGTGTTGAGGAGTTGCCTCTTGATCCTTTACCTCGATCTGGATCTTTTTCTCCTCATTTGAATCAACACACACTTCCAACAGATTCTCCCGACAGGTTCAGGGAACCATATGATGGCGTAGGTGGCCCTGCTGGACTCTGGCATTTTGTATATCGTAGTATCTTTTTGGATCAATATGTATCTTCTGAGTTCTCAGCACCAATAAATAGTCCTCGTCAGCAGAAAAGATTATATAGAGCTTACCAGAAGCTTTACGCTTCCATGCATGAAAGAGGAATTGGGCCCCACAAAACCCAGTTTAGAAGAGATGAGAATTATGGCATCTAG

Protein Analysis

548

Amino Acids

60.23

Weight (kDa)

5.48

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fuz_longin_1 PF19036 164 - 285 1.5e-30 First Longin domain of FUZ, MON1 and HPS1
Fuz_longin_2 PF19037 324 - 417 1.5e-21 Second Longin domain of FUZ, MON1 and HPS1
Fuz_longin_3 PF19038 485 - 545 1.3e-06 Third Longin domain of FUZ, MON1 and HPS1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013229)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28390
fragaria_vesca FvH4_3g32210 FvH4_3g32210 FvH4_3g32210
malus_domestica MD03G1118200.v1.1 MD11G1136600.v1.1
prunus_persica Prupe.6G102100_v2.0.a1 Prupe.6G102100_v2.0.a1
pyrus_communis pycom11g11310
rosa_chinensis RchiOBHm_Chr5g0058821
rosa_laevigata RLG00000035254
rosa_roxburghii Rroxscaffold_1G00021560
rosa_rugosa Rorug05G0319400
rosa_samantha Rh5AG384200 Rh5BG396100 Rh5CG420000 Rh5DG411900
rosa_wichuraiana Rw5G036200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 929
AasI GACNNNNNNGTC 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 929
AccB1I GGYRCC 2 cut(s) 430, 941
AccB7I CCANNNNNTGG 2 cut(s) 474, 1433
AccBSI CCGCTC 1 cut(s) 237
AccII CGCG 4 cut(s) 11, 92, 142, 144
AclWI GGATC 5 cut(s) 368, 1293, 1337, 1369, 1497
AcoI YGGCCR 1 cut(s) 81
AcuI CTGAAG 1 cut(s) 1289
AcyI GRCGYC 1 cut(s) 123
AfiI CCNNNNNNNGG 5 cut(s) 441, 474, 824, 1358, 1433
AgsI TTSAA 2 cut(s) 1256, 1381
AhlI ACTAGT 2 cut(s) 332, 1208
AjnI CCWGG 1 cut(s) 678
AjuI GAANNNNNNNTTGG 2 cut(s) 1581, 1613
AluBI AGCT 6 cut(s) 18, 274, 693, 935, 1559, 1570
AluI AGCT 6 cut(s) 18, 274, 693, 935, 1559, 1570
Alw21I GWGCWC 2 cut(s) 20, 1027
AlwI GGATC 5 cut(s) 368, 1293, 1337, 1369, 1497
AlwNI CAGNNNCTG 1 cut(s) 754
AoxI GGCC 5 cut(s) 81, 279, 287, 1444, 1601
ApaI GGGCCC 1 cut(s) 1605
ApeKI GCWGC 5 cut(s) 303, 306, 693, 754, 935
AspLEI GCGC 3 cut(s) 144, 190, 424
AspS9I GGNCC 7 cut(s) 73, 287, 668, 1261, 1445, 1601, 1602
AsuC2I CCSGG 3 cut(s) 68, 358, 435
AsuHPI GGTGA 1 cut(s) 1107
AvaII GGWCC 3 cut(s) 73, 668, 1261
BaeGI GKGCMC 2 cut(s) 435, 1605
BanI GGYRCC 2 cut(s) 430, 941
BanII GRGCYC 3 cut(s) 20, 299, 1605
BbsI GAAGAC 2 cut(s) 835, 899
Bbv12I GWGCWC 2 cut(s) 20, 1027
BbvI GCAGC 5 cut(s) 315, 318, 680, 741, 922
BccI CCATC 4 cut(s) 344, 395, 1304, 1427
BceAI ACGGC 3 cut(s) 136, 409, 458
BcgI CGANNNNNNTGC 4 cut(s) 117, 151, 353, 387
BciT130I CCWGG 1 cut(s) 680
BcnI CCSGG 3 cut(s) 68, 358, 435
BcuI ACTAGT 2 cut(s) 332, 1208
BfaI CTAG 6 cut(s) 300, 333, 557, 1209, 1307, 1645
BfmI CTRYAG 1 cut(s) 936
BfoI RGCGCY 2 cut(s) 191, 425
BfuAI ACCTGC 1 cut(s) 929
Bme1390I CCNGG 4 cut(s) 68, 358, 435, 680
Bme18I GGWCC 3 cut(s) 73, 668, 1261
BmgT120I GGNCC 7 cut(s) 73, 287, 668, 1261, 1445, 1601, 1602
BmiI GGNNCC 8 cut(s) 204, 355, 432, 669, 943, 1425, 1603, 1604
BmrFI CCNGG 4 cut(s) 68, 358, 435, 680
BmrI ACTGGG 1 cut(s) 1609
BmsI GCATC 7 cut(s) 451, 652, 696, 741, 1043, 1091, 1168
BmuI ACTGGG 1 cut(s) 1609
BpiI GAAGAC 2 cut(s) 835, 899
BpmI CTGGAG 1 cut(s) 296
BpuMI CCSGG 3 cut(s) 68, 358, 435
Bsa29I ATCGAT 1 cut(s) 363
BsaBI GATNNNNATC 1 cut(s) 1360
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 66, 425, 1143
BsaWI WCCGGW 1 cut(s) 209
BsaXI ACNNNNNCTCC 6 cut(s) 17, 47, 603, 633, 1390, 1420
Bsc4I CCNNNNNNNGG 5 cut(s) 441, 474, 824, 1358, 1433
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 2 cut(s) 342, 1615
Bse8I GATNNNNATC 1 cut(s) 1360
BseBI CCWGG 1 cut(s) 680
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 3 cut(s) 66, 425, 1143
BseGI GGATG 3 cut(s) 463, 855, 1042
BseJI GATNNNNATC 1 cut(s) 1360
BseLI CCNNNNNNNGG 5 cut(s) 441, 474, 824, 1358, 1433
BseMII CTCAG 5 cut(s) 210, 1040, 1297, 1497, 1527
BseNI ACTGG 2 cut(s) 342, 1615
BseRI GAGGAG 4 cut(s) 191, 274, 1343, 1362
BseSI GKGCMC 2 cut(s) 435, 1605
BseXI GCAGC 5 cut(s) 315, 318, 680, 741, 922
BseYI CCCAGC 2 cut(s) 635, 823
BsgI GTGCAG 1 cut(s) 679
Bsh1236I CGCG 4 cut(s) 11, 92, 142, 144
BshFI GGCC 5 cut(s) 83, 281, 289, 1446, 1603
BshNI GGYRCC 2 cut(s) 430, 941
BshVI ATCGAT 1 cut(s) 363
BsiHKAI GWGCWC 2 cut(s) 20, 1027
BsiSI CCGG 7 cut(s) 21, 68, 210, 290, 318, 357, 435
BslFI GGGAC 2 cut(s) 343, 681
BslI CCNNNNNNNGG 5 cut(s) 441, 474, 824, 1358, 1433
BsmFI GGGAC 2 cut(s) 343, 681
BsmI GAATGC 2 cut(s) 729, 1323
BsnI GGCC 5 cut(s) 83, 281, 289, 1446, 1603
Bsp120I GGGCCC 1 cut(s) 1601
Bsp1286I GDGCHC 5 cut(s) 20, 299, 435, 1027, 1605
Bsp143I GATC 8 cut(s) 162, 360, 613, 1298, 1342, 1355, 1361, 1489
Bsp19I CCATGG 1 cut(s) 1143
BspANI GGCC 5 cut(s) 83, 281, 289, 1446, 1603
BspCNI CTCAG 5 cut(s) 211, 1039, 1296, 1498, 1526
BspDI ATCGAT 1 cut(s) 363
BspFNI CGCG 4 cut(s) 11, 92, 142, 144
BspLI GGNNCC 8 cut(s) 204, 355, 432, 669, 943, 1425, 1603, 1604
BspMAI CTGCAG 1 cut(s) 940
BspMI ACCTGC 1 cut(s) 929
BspPI GGATC 5 cut(s) 368, 1293, 1337, 1369, 1497
BspQI GCTCTTC 1 cut(s) 696
BspT107I GGYRCC 2 cut(s) 430, 941
BsrBI CCGCTC 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 2 cut(s) 342, 1615
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 3 cut(s) 66, 425, 1143
BssMI GATC 8 cut(s) 162, 360, 613, 1298, 1342, 1355, 1361, 1489
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 1143
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 1 cut(s) 746
Bst6I CTCTTC 5 cut(s) 168, 696, 853, 1044, 1618
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 693
BstC8I GCNNGC 3 cut(s) 319, 933, 1127
BstDEI CTNAG 6 cut(s) 219, 506, 1026, 1283, 1506, 1513
BstDSI CCRYGG 2 cut(s) 425, 1143
BstF5I GGATG 3 cut(s) 463, 855, 1042
BstFNI CGCG 4 cut(s) 11, 92, 142, 144
BstH2I RGCGCY 2 cut(s) 191, 425
BstHHI GCGC 3 cut(s) 144, 190, 424
BstKTI GATC 8 cut(s) 165, 363, 616, 1301, 1345, 1358, 1364, 1492
BstMBI GATC 8 cut(s) 162, 360, 613, 1298, 1342, 1355, 1361, 1489
BstMWI GCNNNNNNNGC 9 cut(s) 89, 303, 309, 318, 430, 693, 941, 1031, 1443
BstNI CCWGG 1 cut(s) 680
BstNSI RCATGY 2 cut(s) 449, 1057
BstSCI CCNGG 4 cut(s) 66, 356, 433, 678
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 2 cut(s) 435, 1605
BstUI CGCG 4 cut(s) 11, 92, 142, 144
BstV1I GCAGC 5 cut(s) 315, 318, 680, 741, 922
BstV2I GAAGAC 2 cut(s) 835, 899
BstX2I RGATCY 2 cut(s) 1298, 1361
BstYI RGATCY 2 cut(s) 1298, 1361
Bsu15I ATCGAT 1 cut(s) 363
BsuRI GGCC 5 cut(s) 83, 281, 289, 1446, 1603
BsuTUI ATCGAT 1 cut(s) 363
BtgI CCRYGG 2 cut(s) 425, 1143
BtsCI GGATG 3 cut(s) 463, 855, 1042
BtsIMutI CAGTG 1 cut(s) 1291
BveI ACCTGC 1 cut(s) 929
Cac8I GCNNGC 3 cut(s) 319, 933, 1127
CaiI CAGNNNCTG 1 cut(s) 754
CfoI GCGC 3 cut(s) 144, 190, 424
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 7 cut(s) 73, 287, 668, 1261, 1445, 1601, 1602
ClaI ATCGAT 1 cut(s) 363
CseI GACGC 1 cut(s) 112
CsiI ACCWGGT 1 cut(s) 678
CspCI CAANNNNNGTGG 2 cut(s) 407, 442
CviAII CATG 9 cut(s) 446, 474, 539, 893, 1054, 1144, 1303, 1582, 1586
DdeI CTNAG 6 cut(s) 219, 506, 1026, 1283, 1506, 1513
DpnI GATC 8 cut(s) 164, 362, 615, 1300, 1344, 1357, 1363, 1491
DpnII GATC 8 cut(s) 162, 360, 613, 1298, 1342, 1355, 1361, 1489
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 5 cut(s) 168, 696, 853, 1044, 1618
EarI CTCTTC 5 cut(s) 168, 696, 853, 1044, 1618
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 1143
Eco24I GRGCYC 3 cut(s) 20, 299, 1605
Eco47I GGWCC 3 cut(s) 73, 668, 1261
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 1289
EcoICRI GAGCTC 1 cut(s) 18
EcoO109I RGGNCCY 1 cut(s) 1602
EcoRII CCWGG 1 cut(s) 678
EcoT14I CCWWGG 1 cut(s) 1143
EcoT22I ATGCAT 3 cut(s) 689, 898, 1587
EcoT38I GRGCYC 3 cut(s) 20, 299, 1605
ErhI CCWWGG 1 cut(s) 1143
FaeI CATG 9 cut(s) 449, 477, 542, 896, 1057, 1147, 1306, 1585, 1589
FalI AAGNNNNNCTT 4 cut(s) 479, 511, 1023, 1055
FaqI GGGAC 2 cut(s) 343, 681
FatI CATG 9 cut(s) 445, 473, 538, 892, 1053, 1143, 1302, 1581, 1585
FauI CCCGC 1 cut(s) 137
FauNDI CATATG 1 cut(s) 1429
FokI GGATG 3 cut(s) 470, 842, 1029
FriOI GRGCYC 3 cut(s) 20, 299, 1605
FspBI CTAG 6 cut(s) 300, 333, 557, 1209, 1307, 1645
GlaI GCGC 3 cut(s) 143, 189, 423
GsaI CCCAGC 2 cut(s) 639, 827
GsuI CTGGAG 1 cut(s) 296
HaeII RGCGCY 2 cut(s) 191, 425
HaeIII GGCC 5 cut(s) 83, 281, 289, 1446, 1603
HapII CCGG 7 cut(s) 21, 68, 210, 290, 318, 357, 435
HgaI GACGC 1 cut(s) 112
HhaI GCGC 3 cut(s) 144, 190, 424
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 9 cut(s) 449, 477, 542, 896, 1057, 1147, 1306, 1585, 1589
Hin6I GCGC 3 cut(s) 142, 188, 422
HinP1I GCGC 3 cut(s) 142, 188, 422
HindIII AAGCTT 1 cut(s) 1568
HpaII CCGG 7 cut(s) 21, 68, 210, 290, 318, 357, 435
HphI GGTGA 1 cut(s) 1107
Hpy188III TCNNGA 6 cut(s) 166, 534, 1046, 1340, 1359, 1409
Hpy99I CGWCG 4 cut(s) 16, 29, 128, 287
HpyAV CCTTC 2 cut(s) 1231, 1274
HpyCH4III ACNGT 1 cut(s) 746
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 303, 309, 318, 430, 693, 941, 1031, 1443
HpyF3I CTNAG 6 cut(s) 219, 506, 1026, 1283, 1506, 1513
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 9 cut(s) 449, 477, 542, 896, 1057, 1147, 1306, 1585, 1589
HspAI GCGC 3 cut(s) 142, 188, 422
KroI GCCGGC 1 cut(s) 317
KroNI GCCGGC 1 cut(s) 319
Kzo9I GATC 8 cut(s) 162, 360, 613, 1298, 1342, 1355, 1361, 1489
LguI GCTCTTC 1 cut(s) 696
LmnI GCTCC 4 cut(s) 23, 208, 261, 271
Lsp1109I GCAGC 5 cut(s) 315, 318, 680, 741, 922
LweI GCATC 7 cut(s) 451, 652, 696, 741, 1043, 1091, 1168
MabI ACCWGGT 1 cut(s) 678
MaeI CTAG 6 cut(s) 300, 333, 557, 1209, 1307, 1645
MaeIII GTNAC 1 cut(s) 509
MalI GATC 8 cut(s) 164, 362, 615, 1300, 1344, 1357, 1363, 1491
MbiI CCGCTC 1 cut(s) 237
MboI GATC 8 cut(s) 162, 360, 613, 1298, 1342, 1355, 1361, 1489
MfeI CAATTG 2 cut(s) 77, 728
MflI RGATCY 2 cut(s) 1298, 1361
MhlI GDGCHC 5 cut(s) 20, 299, 435, 1027, 1605
MlyI GAGTC 2 cut(s) 722, 1449
MmeI TCCRAC 5 cut(s) 60, 123, 851, 1092, 1421
Mph1103I ATGCAT 3 cut(s) 689, 898, 1587
MroNI GCCGGC 1 cut(s) 317
MseI TTAA 2 cut(s) 719, 1233
MslI CAYNNNNRTG 3 cut(s) 450, 543, 1047
MspA1I CMGCKG 2 cut(s) 309, 693
MspI CCGG 7 cut(s) 21, 68, 210, 290, 318, 357, 435
MspR9I CCNGG 4 cut(s) 68, 358, 435, 680
MunI CAATTG 2 cut(s) 77, 728
Mva1269I GAATGC 2 cut(s) 729, 1323
MvaI CCWGG 1 cut(s) 680
MvnI CGCG 4 cut(s) 11, 92, 142, 144
MwoI GCNNNNNNNGC 9 cut(s) 89, 303, 309, 318, 430, 693, 941, 1031, 1443
NaeI GCCGGC 1 cut(s) 319
NciI CCSGG 3 cut(s) 68, 358, 435
NcoI CCATGG 1 cut(s) 1143
NdeI CATATG 1 cut(s) 1429
NdeII GATC 8 cut(s) 162, 360, 613, 1298, 1342, 1355, 1361, 1489
NgoMIV GCCGGC 1 cut(s) 317
NlaIII CATG 9 cut(s) 449, 477, 542, 896, 1057, 1147, 1306, 1585, 1589
NlaIV GGNNCC 8 cut(s) 204, 355, 432, 669, 943, 1425, 1603, 1604
NsiI ATGCAT 3 cut(s) 689, 898, 1587
NspI RCATGY 2 cut(s) 449, 1057
PaqCI CACCTGC 1 cut(s) 929
PciSI GCTCTTC 1 cut(s) 696
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 96
PctI GAATGC 2 cut(s) 729, 1323
PdiI GCCGGC 1 cut(s) 319
PflMI CCANNNNNTGG 2 cut(s) 474, 1433
PfoI TCCNGGA 1 cut(s) 356
PleI GAGTC 2 cut(s) 721, 1449
PpsI GAGTC 2 cut(s) 721, 1449
Psp124BI GAGCTC 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 678
PspFI CCCAGC 2 cut(s) 635, 823
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 8 cut(s) 204, 355, 432, 669, 943, 1425, 1603, 1604
PspOMI GGGCCC 1 cut(s) 1601
PspPI GGNCC 7 cut(s) 73, 287, 668, 1261, 1445, 1601, 1602
PsrI GAACNNNNNNTAC 2 cut(s) 337, 369
PstI CTGCAG 1 cut(s) 940
PstNI CAGNNNCTG 1 cut(s) 754
PsuI RGATCY 2 cut(s) 1298, 1361
PvuII CAGCTG 1 cut(s) 693
RseI CAYNNNNRTG 3 cut(s) 450, 543, 1047
SacI GAGCTC 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 696
SaqAI TTAA 2 cut(s) 719, 1233
Sau3AI GATC 8 cut(s) 162, 360, 613, 1298, 1342, 1355, 1361, 1489
Sau96I GGNCC 7 cut(s) 73, 287, 668, 1261, 1445, 1601, 1602
SchI GAGTC 2 cut(s) 722, 1449
ScrFI CCNGG 4 cut(s) 68, 358, 435, 680
SduI GDGCHC 5 cut(s) 20, 299, 435, 1027, 1605
SexAI ACCWGGT 1 cut(s) 678
SfaNI GCATC 7 cut(s) 451, 652, 696, 741, 1043, 1091, 1168
SfcI CTRYAG 1 cut(s) 936
SinI GGWCC 3 cut(s) 73, 668, 1261
SmiMI CAYNNNNRTG 3 cut(s) 450, 543, 1047
SpeI ACTAGT 2 cut(s) 332, 1208
SspI AATATT 1 cut(s) 987
SspMI CTAG 6 cut(s) 300, 333, 557, 1209, 1307, 1645
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 4 cut(s) 66, 356, 433, 678
StyI CCWWGG 1 cut(s) 1143
TaaI ACNGT 1 cut(s) 746
TaqI TCGA 7 cut(s) 14, 165, 233, 363, 1176, 1274, 1354
TauI GCSGC 6 cut(s) 86, 142, 185, 188, 312, 324
Tru1I TTAA 2 cut(s) 719, 1233
Tru9I TTAA 2 cut(s) 719, 1233
TscAI CASTG 1 cut(s) 1291
TseI GCWGC 5 cut(s) 303, 306, 693, 754, 935
TspDTI ATGAA 6 cut(s) 147, 490, 561, 881, 1031, 1602
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 1291
Van91I CCANNNNNTGG 2 cut(s) 474, 1433
VpaK11BI GGWCC 3 cut(s) 73, 668, 1261
XceI RCATGY 2 cut(s) 449, 1057
XcmI CCANNNNNNNNNTGG 1 cut(s) 1075
XspI CTAG 6 cut(s) 300, 333, 557, 1209, 1307, 1645
Zsp2I ATGCAT 3 cut(s) 689, 898, 1587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.