Rh5BG396100

Vacuolar fusion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0013229
Physical Location & Seq
Reverse (-)
63961959 .. 63970695
8737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG396100.1

Sequence Viewer

Length: 1656 bp
ATGACCTCCGCGTCGAGCTCCGGCGACGAAACCTCCGACCCGAAGCCCAACCCGATTACAAAGCCCCCGGAGGACCAATTGGCCGCCCTCGCGTTATCCGACACCGATAGCCAAGCAAACGGCGTCGCAAATGAATCGCCGCGCGGGAACAATCACAATGAGATCGAGAACGAAGAGGAGGTGGCAGCGCTGACCAATTCGGCTCCGTCTGGTTCGTCTGAGGTGGTGGAATCGAGCGGTGGTGGAGGTGGTGGTATTCTGCGGGCGAGGAGCAATTCGGAGCTGGAGGCCGACGGGCCGGAGAGCCCTAGCAGCAGCGGCTATGCCGGCGGCAGAGGGAGTAGTAGTGCCAGTAGTGATGGTTCCGGGATCGATGAGATTGCGGCAGTTAGTGATGTTGAGGTTGTTGATGGTGTTTCGGATTCACAAGCGCCGTGGGTGCCCGGAAAACGGCATGTTGATGAGGATGATGCTTCCATATCATGGAGAAAAAGGAAGAAGCACTTTTTCATACTAAGTAACTCTGGCAAACCAATATATTCCAGACATGGAGATGAACACAAACTAGCAGGATTTTCAGCAACATTACAAGCCATAATTTCCTTTGTTGAGAATGGTGGAGATCGTGTCAAATTGGTTAGGGCTGGGAAGCATCAGGTGGTTTTTCTTGTCAAGGGACCAATTTACCTGGTATGCATCAGCTGCACAGAAGAGCCTTATGAGTCTTTAAGAATGCAATTGGAACTTATATACGGTCAGATGCTGCTTATTCTTACGAAGTCAGTAAATAGATGTTTTGAGAAGAATCCGAAGTTTGATATGACACCTTTGCTGGGAGGAACAGATATTGTCTTCTCTTCCCTCATCCATTCTTTCAGTTGGAATCCTGCCACATTTCTTCATGCATATACTTGTCTTCCCCTTGCTTATGCGACAAGGCAAGCTGCAGGTGCCATATTGCAAGATGTTGCTGATTCAGGTGTCCTCTTTGCAATATTGATGTGCAAACACAAGGTTATCAGTCTTGTTGGTGCTCAGAAAGCGTCTCTTCATCCTGATGACATGCTATTGCTATCCAACTTTGTTATGGCATCAGAATCATTTAGGACATCTGAATCTTTCTCACCAATTTGCTTGCCAAGATATAATGCCATGGCATTTTTGTATGCTTATGTCCATTTTTTCGATGCTGACACATACTTGATGTTGCTTACTACTAGTTCAGAAGCCTTCTATCATCTTAAAGATTGCAGGATTCGTATTGAAATGGTCCTTCTGAAGTCGAGTGTCCTCAGTGAGATTCAGAGATCCATGCTAGATGGTGGAATGCGTGTTGAGGAGTTGCCTCTTGATCCTTTACCTCGATCTGGATCTTTTTCTCCTCATTTGAACCAACACACACTTCCAACAGATTCTCCCGACAGGTTCAGGGAACCATATGATGGCGTAGGTGGCCCTGCTGGACTCTGGCATTTTGTATATCGTAGTATCTTTTTGGATCAATATGTATCTTCTGAGTTCTCAGCACCAATAAATAGTGCTCGTCAGCAGAAAAGATTATATAGAGCTTACCAGAAGCTTTACGCTTCCATGCATGAAAGAGGAATTGGGCCCCACAAAACCCAGTTTAGAAGAGATGAGAATTATGGCATCTAG

Protein Analysis

551

Amino Acids

60.33

Weight (kDa)

5.61

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fuz_longin_1 PF19036 167 - 287 4.9e-30 First Longin domain of FUZ, MON1 and HPS1
Fuz_longin_2 PF19037 327 - 420 1.5e-21 Second Longin domain of FUZ, MON1 and HPS1
Fuz_longin_3 PF19038 488 - 548 6.8e-07 Third Longin domain of FUZ, MON1 and HPS1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013229)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28390
fragaria_vesca FvH4_3g32210 FvH4_3g32210 FvH4_3g32210
malus_domestica MD03G1118200.v1.1 MD11G1136600.v1.1
prunus_persica Prupe.6G102100_v2.0.a1 Prupe.6G102100_v2.0.a1
pyrus_communis pycom11g11310
rosa_chinensis RchiOBHm_Chr5g0058821
rosa_laevigata RLG00000035254
rosa_roxburghii Rroxscaffold_1G00021560
rosa_rugosa Rorug05G0319400
rosa_samantha Rh5AG384200 Rh5BG396100 Rh5CG420000 Rh5DG411900
rosa_wichuraiana Rw5G036200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 938
AasI GACNNNNNNGTC 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 938
AccB1I GGYRCC 2 cut(s) 439, 950
AccB7I CCANNNNNTGG 2 cut(s) 483, 1442
AccBSI CCGCTC 1 cut(s) 237
AccII CGCG 4 cut(s) 11, 92, 142, 144
AciI CCGC 9 cut(s) 9, 84, 140, 144, 237, 262, 318, 330, 383
AclWI GGATC 5 cut(s) 377, 1302, 1346, 1378, 1506
AcoI YGGCCR 1 cut(s) 81
AcuI CTGAAG 1 cut(s) 1298
AcyI GRCGYC 1 cut(s) 123
AfeI AGCGCT 1 cut(s) 189
AfiI CCNNNNNNNGG 5 cut(s) 450, 483, 833, 1367, 1442
AgsI TTSAA 2 cut(s) 1265, 1390
AhlI ACTAGT 1 cut(s) 1217
AjnI CCWGG 1 cut(s) 687
AjuI GAANNNNNNNTTGG 4 cut(s) 1386, 1418, 1590, 1622
AluBI AGCT 6 cut(s) 18, 283, 702, 944, 1568, 1579
AluI AGCT 6 cut(s) 18, 283, 702, 944, 1568, 1579
Alw21I GWGCWC 3 cut(s) 20, 1036, 1543
Alw26I GTCTC 1 cut(s) 1050
AlwI GGATC 5 cut(s) 377, 1302, 1346, 1378, 1506
AlwNI CAGNNNCTG 1 cut(s) 763
Aor51HI AGCGCT 1 cut(s) 189
AoxI GGCC 5 cut(s) 81, 288, 296, 1453, 1610
ApaI GGGCCC 1 cut(s) 1614
ApeKI GCWGC 6 cut(s) 185, 312, 315, 702, 763, 944
AspLEI GCGC 3 cut(s) 144, 190, 433
AspS9I GGNCC 7 cut(s) 73, 296, 677, 1270, 1454, 1610, 1611
AsuC2I CCSGG 3 cut(s) 68, 367, 444
AsuHPI GGTGA 1 cut(s) 1116
AvaII GGWCC 3 cut(s) 73, 677, 1270
BaeGI GKGCMC 2 cut(s) 444, 1614
BanI GGYRCC 2 cut(s) 439, 950
BanII GRGCYC 3 cut(s) 20, 308, 1614
BbsI GAAGAC 2 cut(s) 844, 908
Bbv12I GWGCWC 3 cut(s) 20, 1036, 1543
BbvI GCAGC 6 cut(s) 197, 324, 327, 689, 750, 931
BccI CCATC 4 cut(s) 353, 404, 1313, 1436
BceAI ACGGC 3 cut(s) 136, 418, 467
BcgI CGANNNNNNTGC 4 cut(s) 117, 151, 362, 396
BciT130I CCWGG 1 cut(s) 689
BcnI CCSGG 3 cut(s) 68, 367, 444
BcoDI GTCTC 1 cut(s) 1050
BcuI ACTAGT 1 cut(s) 1217
BfaI CTAG 5 cut(s) 309, 566, 1218, 1316, 1654
BfmI CTRYAG 1 cut(s) 945
BfoI RGCGCY 2 cut(s) 191, 434
BfuAI ACCTGC 1 cut(s) 938
Bme1390I CCNGG 4 cut(s) 68, 367, 444, 689
Bme18I GGWCC 3 cut(s) 73, 677, 1270
BmgT120I GGNCC 7 cut(s) 73, 296, 677, 1270, 1454, 1610, 1611
BmiI GGNNCC 8 cut(s) 204, 364, 441, 678, 952, 1434, 1612, 1613
BmrFI CCNGG 4 cut(s) 68, 367, 444, 689
BmrI ACTGGG 1 cut(s) 1618
BmsI GCATC 6 cut(s) 460, 661, 705, 750, 1100, 1177
BmuI ACTGGG 1 cut(s) 1618
BpiI GAAGAC 2 cut(s) 844, 908
BpmI CTGGAG 1 cut(s) 305
BpuMI CCSGG 3 cut(s) 68, 367, 444
Bsa29I ATCGAT 1 cut(s) 372
BsaBI GATNNNNATC 1 cut(s) 1369
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 66, 434, 1152
BsaXI ACNNNNNCTCC 8 cut(s) 17, 47, 331, 361, 612, 642, 1399, 1429
Bsc4I CCNNNNNNNGG 5 cut(s) 450, 483, 833, 1367, 1442
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 2 cut(s) 351, 1624
Bse8I GATNNNNATC 1 cut(s) 1369
BseBI CCWGG 1 cut(s) 689
BseCI ATCGAT 1 cut(s) 372
BseDI CCNNGG 3 cut(s) 66, 434, 1152
BseGI GGATG 3 cut(s) 472, 864, 1051
BseJI GATNNNNATC 1 cut(s) 1369
BseLI CCNNNNNNNGG 5 cut(s) 450, 483, 833, 1367, 1442
BseMII CTCAG 5 cut(s) 210, 1049, 1306, 1506, 1536
BseNI ACTGG 2 cut(s) 351, 1624
BseRI GAGGAG 4 cut(s) 191, 283, 1352, 1371
BseSI GKGCMC 2 cut(s) 444, 1614
BseXI GCAGC 6 cut(s) 197, 324, 327, 689, 750, 931
BseYI CCCAGC 2 cut(s) 644, 832
BsgI GTGCAG 1 cut(s) 688
Bsh1236I CGCG 4 cut(s) 11, 92, 142, 144
BshFI GGCC 5 cut(s) 83, 290, 298, 1455, 1612
BshNI GGYRCC 2 cut(s) 439, 950
BshVI ATCGAT 1 cut(s) 372
BsiHKAI GWGCWC 3 cut(s) 20, 1036, 1543
BsiSI CCGG 6 cut(s) 21, 68, 299, 327, 366, 444
BslFI GGGAC 1 cut(s) 690
BslI CCNNNNNNNGG 5 cut(s) 450, 483, 833, 1367, 1442
BsmAI GTCTC 1 cut(s) 1050
BsmBI CGTCTC 1 cut(s) 1050
BsmFI GGGAC 1 cut(s) 690
BsmI GAATGC 2 cut(s) 738, 1332
BsnI GGCC 5 cut(s) 83, 290, 298, 1455, 1612
Bsp120I GGGCCC 1 cut(s) 1610
Bsp1286I GDGCHC 6 cut(s) 20, 308, 444, 1036, 1543, 1614
Bsp143I GATC 8 cut(s) 162, 369, 622, 1307, 1351, 1364, 1370, 1498
Bsp19I CCATGG 1 cut(s) 1152
BspACI CCGC 9 cut(s) 9, 84, 140, 144, 237, 262, 318, 330, 383
BspANI GGCC 5 cut(s) 83, 290, 298, 1455, 1612
BspCNI CTCAG 5 cut(s) 211, 1048, 1305, 1507, 1535
BspDI ATCGAT 1 cut(s) 372
BspFNI CGCG 4 cut(s) 11, 92, 142, 144
BspLI GGNNCC 8 cut(s) 204, 364, 441, 678, 952, 1434, 1612, 1613
BspMAI CTGCAG 1 cut(s) 949
BspMI ACCTGC 1 cut(s) 938
BspPI GGATC 5 cut(s) 377, 1302, 1346, 1378, 1506
BspQI GCTCTTC 1 cut(s) 705
BspT107I GGYRCC 2 cut(s) 439, 950
BsrBI CCGCTC 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 2 cut(s) 351, 1624
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 3 cut(s) 66, 434, 1152
BssMI GATC 8 cut(s) 162, 369, 622, 1307, 1351, 1364, 1370, 1498
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 1152
Bst2UI CCWGG 1 cut(s) 689
Bst4CI ACNGT 1 cut(s) 755
Bst6I CTCTTC 5 cut(s) 168, 705, 862, 1053, 1627
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 702
BstC8I GCNNGC 4 cut(s) 264, 328, 942, 1136
BstDEI CTNAG 6 cut(s) 219, 515, 1035, 1292, 1515, 1522
BstDSI CCRYGG 2 cut(s) 434, 1152
BstF5I GGATG 3 cut(s) 472, 864, 1051
BstFNI CGCG 4 cut(s) 11, 92, 142, 144
BstH2I RGCGCY 2 cut(s) 191, 434
BstHHI GCGC 3 cut(s) 144, 190, 433
BstKTI GATC 8 cut(s) 165, 372, 625, 1310, 1354, 1367, 1373, 1501
BstMAI GTCTC 1 cut(s) 1050
BstMBI GATC 8 cut(s) 162, 369, 622, 1307, 1351, 1364, 1370, 1498
BstMWI GCNNNNNNNGC 9 cut(s) 89, 312, 318, 327, 439, 702, 950, 1040, 1452
BstNI CCWGG 1 cut(s) 689
BstNSI RCATGY 2 cut(s) 458, 1066
BstSCI CCNGG 4 cut(s) 66, 365, 442, 687
BstSFI CTRYAG 1 cut(s) 945
BstSLI GKGCMC 2 cut(s) 444, 1614
BstUI CGCG 4 cut(s) 11, 92, 142, 144
BstV1I GCAGC 6 cut(s) 197, 324, 327, 689, 750, 931
BstV2I GAAGAC 2 cut(s) 844, 908
BstX2I RGATCY 2 cut(s) 1307, 1370
BstYI RGATCY 2 cut(s) 1307, 1370
Bsu15I ATCGAT 1 cut(s) 372
BsuRI GGCC 5 cut(s) 83, 290, 298, 1455, 1612
BsuTUI ATCGAT 1 cut(s) 372
BtgI CCRYGG 2 cut(s) 434, 1152
BtsCI GGATG 3 cut(s) 472, 864, 1051
BtsIMutI CAGTG 1 cut(s) 1300
BveI ACCTGC 1 cut(s) 938
Cac8I GCNNGC 4 cut(s) 264, 328, 942, 1136
CaiI CAGNNNCTG 1 cut(s) 763
CfoI GCGC 3 cut(s) 144, 190, 433
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 7 cut(s) 73, 296, 677, 1270, 1454, 1610, 1611
ClaI ATCGAT 1 cut(s) 372
CseI GACGC 2 cut(s) 112, 1032
CsiI ACCWGGT 1 cut(s) 687
CspCI CAANNNNNGTGG 2 cut(s) 416, 451
CviAII CATG 9 cut(s) 455, 483, 548, 902, 1063, 1153, 1312, 1591, 1595
DdeI CTNAG 6 cut(s) 219, 515, 1035, 1292, 1515, 1522
DpnI GATC 8 cut(s) 164, 371, 624, 1309, 1353, 1366, 1372, 1500
DpnII GATC 8 cut(s) 162, 369, 622, 1307, 1351, 1364, 1370, 1498
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 5 cut(s) 168, 705, 862, 1053, 1627
EarI CTCTTC 5 cut(s) 168, 705, 862, 1053, 1627
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 1152
Eco24I GRGCYC 3 cut(s) 20, 308, 1614
Eco47I GGWCC 3 cut(s) 73, 677, 1270
Eco47III AGCGCT 1 cut(s) 189
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 1298
EcoICRI GAGCTC 1 cut(s) 18
EcoO109I RGGNCCY 1 cut(s) 1611
EcoRII CCWGG 1 cut(s) 687
EcoT14I CCWWGG 1 cut(s) 1152
EcoT22I ATGCAT 3 cut(s) 698, 907, 1596
EcoT38I GRGCYC 3 cut(s) 20, 308, 1614
ErhI CCWWGG 1 cut(s) 1152
Esp3I CGTCTC 1 cut(s) 1050
FaeI CATG 9 cut(s) 458, 486, 551, 905, 1066, 1156, 1315, 1594, 1598
FalI AAGNNNNNCTT 4 cut(s) 488, 520, 1032, 1064
FaqI GGGAC 1 cut(s) 690
FatI CATG 9 cut(s) 454, 482, 547, 901, 1062, 1152, 1311, 1590, 1594
FauI CCCGC 2 cut(s) 137, 255
FauNDI CATATG 1 cut(s) 1438
FokI GGATG 3 cut(s) 479, 851, 1038
FriOI GRGCYC 3 cut(s) 20, 308, 1614
FspBI CTAG 5 cut(s) 309, 566, 1218, 1316, 1654
GlaI GCGC 3 cut(s) 143, 189, 432
GsaI CCCAGC 2 cut(s) 648, 836
GsuI CTGGAG 1 cut(s) 305
HaeII RGCGCY 2 cut(s) 191, 434
HaeIII GGCC 5 cut(s) 83, 290, 298, 1455, 1612
HapII CCGG 6 cut(s) 21, 68, 299, 327, 366, 444
HgaI GACGC 2 cut(s) 112, 1032
HhaI GCGC 3 cut(s) 144, 190, 433
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 9 cut(s) 458, 486, 551, 905, 1066, 1156, 1315, 1594, 1598
Hin6I GCGC 3 cut(s) 142, 188, 431
HinP1I GCGC 3 cut(s) 142, 188, 431
HindIII AAGCTT 1 cut(s) 1577
HpaII CCGG 6 cut(s) 21, 68, 299, 327, 366, 444
HphI GGTGA 1 cut(s) 1116
Hpy188III TCNNGA 6 cut(s) 166, 543, 1055, 1349, 1368, 1418
Hpy99I CGWCG 4 cut(s) 16, 29, 128, 296
HpyAV CCTTC 2 cut(s) 1240, 1283
HpyCH4III ACNGT 1 cut(s) 755
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 312, 318, 327, 439, 702, 950, 1040, 1452
HpyF3I CTNAG 6 cut(s) 219, 515, 1035, 1292, 1515, 1522
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 9 cut(s) 458, 486, 551, 905, 1066, 1156, 1315, 1594, 1598
HspAI GCGC 3 cut(s) 142, 188, 431
KroI GCCGGC 1 cut(s) 326
KroNI GCCGGC 1 cut(s) 328
Kzo9I GATC 8 cut(s) 162, 369, 622, 1307, 1351, 1364, 1370, 1498
LguI GCTCTTC 1 cut(s) 705
LmnI GCTCC 4 cut(s) 23, 208, 270, 280
Lsp1109I GCAGC 6 cut(s) 197, 324, 327, 689, 750, 931
LweI GCATC 6 cut(s) 460, 661, 705, 750, 1100, 1177
MabI ACCWGGT 1 cut(s) 687
MaeI CTAG 5 cut(s) 309, 566, 1218, 1316, 1654
MaeIII GTNAC 1 cut(s) 518
MalI GATC 8 cut(s) 164, 371, 624, 1309, 1353, 1366, 1372, 1500
MbiI CCGCTC 1 cut(s) 237
MboI GATC 8 cut(s) 162, 369, 622, 1307, 1351, 1364, 1370, 1498
MfeI CAATTG 2 cut(s) 77, 737
MflI RGATCY 2 cut(s) 1307, 1370
MhlI GDGCHC 6 cut(s) 20, 308, 444, 1036, 1543, 1614
MlyI GAGTC 2 cut(s) 731, 1458
MmeI TCCRAC 5 cut(s) 60, 123, 860, 1101, 1430
Mph1103I ATGCAT 3 cut(s) 698, 907, 1596
MroNI GCCGGC 1 cut(s) 326
MseI TTAA 2 cut(s) 728, 1242
MslI CAYNNNNRTG 3 cut(s) 459, 552, 1056
MspA1I CMGCKG 2 cut(s) 318, 702
MspI CCGG 6 cut(s) 21, 68, 299, 327, 366, 444
MspR9I CCNGG 4 cut(s) 68, 367, 444, 689
MunI CAATTG 2 cut(s) 77, 737
Mva1269I GAATGC 2 cut(s) 738, 1332
MvaI CCWGG 1 cut(s) 689
MvnI CGCG 4 cut(s) 11, 92, 142, 144
MwoI GCNNNNNNNGC 9 cut(s) 89, 312, 318, 327, 439, 702, 950, 1040, 1452
NaeI GCCGGC 1 cut(s) 328
NciI CCSGG 3 cut(s) 68, 367, 444
NcoI CCATGG 1 cut(s) 1152
NdeI CATATG 1 cut(s) 1438
NdeII GATC 8 cut(s) 162, 369, 622, 1307, 1351, 1364, 1370, 1498
NgoMIV GCCGGC 1 cut(s) 326
NlaIII CATG 9 cut(s) 458, 486, 551, 905, 1066, 1156, 1315, 1594, 1598
NlaIV GGNNCC 8 cut(s) 204, 364, 441, 678, 952, 1434, 1612, 1613
NsiI ATGCAT 3 cut(s) 698, 907, 1596
NspI RCATGY 2 cut(s) 458, 1066
PaqCI CACCTGC 1 cut(s) 938
PciSI GCTCTTC 1 cut(s) 705
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 96
PctI GAATGC 2 cut(s) 738, 1332
PdiI GCCGGC 1 cut(s) 328
PflMI CCANNNNNTGG 2 cut(s) 483, 1442
PfoI TCCNGGA 1 cut(s) 365
PleI GAGTC 2 cut(s) 730, 1458
PpsI GAGTC 2 cut(s) 730, 1458
Psp124BI GAGCTC 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 687
PspFI CCCAGC 2 cut(s) 644, 832
PspGI CCWGG 1 cut(s) 687
PspN4I GGNNCC 8 cut(s) 204, 364, 441, 678, 952, 1434, 1612, 1613
PspOMI GGGCCC 1 cut(s) 1610
PspPI GGNCC 7 cut(s) 73, 296, 677, 1270, 1454, 1610, 1611
PsrI GAACNNNNNNTAC 2 cut(s) 346, 378
PstI CTGCAG 1 cut(s) 949
PstNI CAGNNNCTG 1 cut(s) 763
PsuI RGATCY 2 cut(s) 1307, 1370
PvuII CAGCTG 1 cut(s) 702
RseI CAYNNNNRTG 3 cut(s) 459, 552, 1056
SacI GAGCTC 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 705
SaqAI TTAA 2 cut(s) 728, 1242
Sau3AI GATC 8 cut(s) 162, 369, 622, 1307, 1351, 1364, 1370, 1498
Sau96I GGNCC 7 cut(s) 73, 296, 677, 1270, 1454, 1610, 1611
SchI GAGTC 2 cut(s) 731, 1458
ScrFI CCNGG 4 cut(s) 68, 367, 444, 689
SduI GDGCHC 6 cut(s) 20, 308, 444, 1036, 1543, 1614
SexAI ACCWGGT 1 cut(s) 687
SfaNI GCATC 6 cut(s) 460, 661, 705, 750, 1100, 1177
SfcI CTRYAG 1 cut(s) 945
SinI GGWCC 3 cut(s) 73, 677, 1270
SmiMI CAYNNNNRTG 3 cut(s) 459, 552, 1056
SpeI ACTAGT 1 cut(s) 1217
SsiI CCGC 9 cut(s) 9, 84, 140, 144, 237, 262, 318, 330, 383
SspI AATATT 1 cut(s) 996
SspMI CTAG 5 cut(s) 309, 566, 1218, 1316, 1654
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 4 cut(s) 66, 365, 442, 687
StyI CCWWGG 1 cut(s) 1152
TaaI ACNGT 1 cut(s) 755
TaqI TCGA 7 cut(s) 14, 165, 233, 372, 1185, 1283, 1363
TauI GCSGC 5 cut(s) 86, 142, 321, 333, 386
Tru1I TTAA 2 cut(s) 728, 1242
Tru9I TTAA 2 cut(s) 728, 1242
TscAI CASTG 1 cut(s) 1300
TseI GCWGC 6 cut(s) 185, 312, 315, 702, 763, 944
TspDTI ATGAA 6 cut(s) 147, 499, 570, 890, 1040, 1611
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 1300
Van91I CCANNNNNTGG 2 cut(s) 483, 1442
VpaK11BI GGWCC 3 cut(s) 73, 677, 1270
XceI RCATGY 2 cut(s) 458, 1066
XcmI CCANNNNNNNNNTGG 1 cut(s) 1084
XspI CTAG 5 cut(s) 309, 566, 1218, 1316, 1654
Zsp2I ATGCAT 3 cut(s) 698, 907, 1596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.