Rroxscaffold_1G00067390

Ubiquitin system component Cue protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016864
Physical Location & Seq
Reverse (-)
88713644 .. 88714237
594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00067390.1

Sequence Viewer

Length: 594 bp
ATGTGTTTCCTTCATCGCTTCATCAATCCAAAAGACTTCGATGCTCTTCTTCTTCGGTTCCTCCTCAACATGTCAATCAACTTCGTCGTCCCGCTGAGAAACCCCGAACCTGATCGTTTCGGAAAGAGAGCGAAACTGACCAATGAGAATCTGAACAATAACCTTAATGGTGCGGAGGAATGGGTCGAGTTGTTTGTTAGAGAGATTAGCAGTGCCATCAGCATCGACGATGCCAAGGCACGTACTGCAAAGGCGCTTGAGGTCTTTGAACAATGGATCAGCTCACGTACTGCCGCCGAGGCTGGGGTGGCAGCCGTCCAGAAGGAGGAGGAGAATATGGTGCTGAAACAAGCTATGGAAGAAATTCAAAAGGAGAATATGATTCTGAAACGAGGCGTATTTATCCAGAACGAGCGTCTTAAAGAATTTGAGGGCCGAAACCAAGAATTGGAGCATGTGAAGCAGTTGGTGAATCAATATAAGGAGAAAGTGAGCGAACTTGAAGTTGCCAACTATGGCTTGGAACTGCACCTCAAGCAAGCTCAGCAATGCAACAACTCTATCCCTTTAAGTTTCCATCCTCATGTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

197

Amino Acids

22.84

Weight (kDa)

5.81

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016864)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g38860 FvH4_6g20801
rosa_chinensis RchiOBHm_Chr5g0009411
rosa_laevigata RLG00000031659
rosa_multiflora Rmu_co7974186.1_g000001
rosa_roxburghii Rroxscaffold_1G00067390
rosa_rugosa Rorug04G0442700
rosa_samantha Rh5AG073300 Rh5AG073900 Rh5BG069700 Rh5CG082100 Rh5CG257800 Rh5DG070000
rosa_wichuraiana Rw5G006850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 448
AciI CCGC 3 cut(s) 92, 173, 294
AclWI GGATC 1 cut(s) 284
AcsI RAATTY 2 cut(s) 363, 425
AfaI GTAC 2 cut(s) 244, 289
AfiI CCNNNNNNNGG 3 cut(s) 303, 325, 448
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 3 cut(s) 269, 368, 503
AluBI AGCT 3 cut(s) 282, 353, 542
AluI AGCT 3 cut(s) 282, 353, 542
AlwI GGATC 1 cut(s) 284
AoxI GGCC 1 cut(s) 433
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 363, 425
Asp700I GAANNNNTTC 1 cut(s) 363
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 433
AsuHPI GGTGA 1 cut(s) 481
BbvI GCAGC 1 cut(s) 323
BccI CCATC 2 cut(s) 224, 585
BceAI ACGGC 1 cut(s) 299
BfoI RGCGCY 1 cut(s) 257
BglI GCCNNNNNGGC 1 cut(s) 299
BisI GCNGC 2 cut(s) 294, 312
BlpI GCTNAGC 1 cut(s) 543
BlsI GCNGC 2 cut(s) 295, 313
BmgT120I GGNCC 1 cut(s) 433
BmiI GGNNCC 1 cut(s) 59
BmsI GCATC 3 cut(s) 31, 220, 231
Bpu1102I GCTNAGC 1 cut(s) 543
BpuEI CTTGAG 2 cut(s) 278, 518
BsaAI YACGTR 2 cut(s) 242, 287
BsaJI CCNNGG 2 cut(s) 234, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 303, 325, 448
Bse3DI GCAATG 1 cut(s) 554
BseDI CCNNGG 2 cut(s) 234, 297
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 3 cut(s) 303, 325, 448
BseMI GCAATG 1 cut(s) 554
BseMII CTCAG 2 cut(s) 86, 557
BseRI GAGGAG 3 cut(s) 53, 341, 344
BseXI GCAGC 1 cut(s) 323
BseYI CCCAGC 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 512
BshFI GGCC 1 cut(s) 435
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 3 cut(s) 303, 325, 448
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 1 cut(s) 435
Bsp143I GATC 2 cut(s) 112, 276
Bsp1720I GCTNAGC 1 cut(s) 543
BspACI CCGC 3 cut(s) 92, 173, 294
BspANI GGCC 1 cut(s) 435
BspCNI CTCAG 2 cut(s) 87, 556
BspLI GGNNCC 1 cut(s) 59
BspPI GGATC 1 cut(s) 284
BspQI GCTCTTC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 554
BssECI CCNNGG 2 cut(s) 234, 297
BssMI GATC 2 cut(s) 112, 276
BssT1I CCWWGG 1 cut(s) 234
Bst6I CTCTTC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 245
BstBAI YACGTR 2 cut(s) 242, 287
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 2 cut(s) 95, 543
BstF5I GGATG 1 cut(s) 577
BstH2I RGCGCY 1 cut(s) 257
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 2 cut(s) 115, 279
BstMBI GATC 2 cut(s) 112, 276
BstMWI GCNNNNNNNGC 6 cut(s) 245, 299, 308, 460, 535, 544
BstNSI RCATGY 2 cut(s) 73, 458
BstV1I GCAGC 1 cut(s) 323
BsuRI GGCC 1 cut(s) 435
BtsCI GGATG 1 cut(s) 577
BtsI GCAGTG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 217
Cac8I GCNNGC 1 cut(s) 540
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 433
CseI GACGC 1 cut(s) 404
Csp6I GTAC 2 cut(s) 243, 288
CviAII CATG 3 cut(s) 70, 455, 584
CviJI RGCY 7 cut(s) 282, 302, 314, 353, 435, 519, 542
CviKI_1 RGCY 7 cut(s) 282, 302, 314, 353, 435, 519, 542
CviQI GTAC 2 cut(s) 243, 288
DdeI CTNAG 2 cut(s) 95, 543
DpnI GATC 2 cut(s) 114, 278
DpnII GATC 2 cut(s) 112, 276
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 3 cut(s) 73, 458, 587
FaiI YATR 8 cut(s) 71, 338, 356, 380, 456, 480, 516, 585
FaqI GGGAC 1 cut(s) 74
FatI CATG 3 cut(s) 69, 454, 583
FauI CCCGC 1 cut(s) 99
Fnu4HI GCNGC 2 cut(s) 294, 312
FokI GGATG 1 cut(s) 564
Fsp4HI GCNGC 2 cut(s) 294, 312
GlaI GCGC 1 cut(s) 255
GluI GCNGC 2 cut(s) 294, 312
GsaI CCCAGC 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 257
HaeIII GGCC 1 cut(s) 435
HgaI GACGC 1 cut(s) 404
HhaI GCGC 1 cut(s) 256
Hin1II CATG 3 cut(s) 73, 458, 587
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HinfI GANTC 3 cut(s) 148, 382, 472
HphI GGTGA 1 cut(s) 481
Hpy188I TCNGA 3 cut(s) 122, 153, 387
Hpy188III TCNNGA 2 cut(s) 319, 406
Hpy99I CGWCG 2 cut(s) 89, 230
HpyAV CCTTC 2 cut(s) 20, 316
HpyCH4IV ACGT 2 cut(s) 241, 286
HpyCH4V TGCA 3 cut(s) 248, 529, 552
HpyF10VI GCNNNNNNNGC 6 cut(s) 245, 299, 308, 460, 535, 544
HpyF3I CTNAG 2 cut(s) 95, 543
HpySE526I ACGT 2 cut(s) 241, 286
Hsp92II CATG 3 cut(s) 73, 458, 587
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 2 cut(s) 112, 276
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 451
LpnPI CCDG 4 cut(s) 123, 288, 332, 419
Lsp1109I GCAGC 1 cut(s) 323
LweI GCATC 3 cut(s) 31, 220, 231
MaeII ACGT 2 cut(s) 241, 286
MalI GATC 2 cut(s) 114, 278
MboI GATC 2 cut(s) 112, 276
MboII GAAGA 4 cut(s) 38, 41, 44, 371
MluCI AATT 3 cut(s) 363, 425, 446
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 3 cut(s) 165, 420, 569
MslI CAYNNNNRTG 1 cut(s) 582
MspA1I CMGCKG 1 cut(s) 94
MwoI GCNNNNNNNGC 6 cut(s) 245, 299, 308, 460, 535, 544
NdeII GATC 2 cut(s) 112, 276
NlaIII CATG 3 cut(s) 73, 458, 587
NlaIV GGNNCC 1 cut(s) 59
NmeAIII GCCGAG 1 cut(s) 322
NspI RCATGY 2 cut(s) 73, 458
PciI ACATGT 1 cut(s) 69
PciSI GCTCTTC 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 3 cut(s) 148, 382, 472
PflMI CCANNNNNTGG 1 cut(s) 448
PkrI GCNGC 2 cut(s) 295, 313
Ppu21I YACGTR 2 cut(s) 242, 287
PscI ACATGT 1 cut(s) 69
PspFI CCCAGC 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 59
PspPI GGNCC 1 cut(s) 433
RsaI GTAC 2 cut(s) 244, 289
RsaNI GTAC 2 cut(s) 243, 288
RseI CAYNNNNRTG 1 cut(s) 582
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 3 cut(s) 165, 420, 569
SatI GCNGC 2 cut(s) 294, 312
Sau3AI GATC 2 cut(s) 112, 276
Sau96I GGNCC 1 cut(s) 433
SetI ASST 9 cut(s) 112, 165, 244, 264, 284, 289, 355, 534, 544
SfaNI GCATC 3 cut(s) 31, 220, 231
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 2 cut(s) 257, 533
SmoI CTYRAG 2 cut(s) 257, 533
Sse9I AATT 3 cut(s) 363, 425, 446
SsiI CCGC 3 cut(s) 92, 173, 294
StyI CCWWGG 1 cut(s) 234
TaiI ACGT 2 cut(s) 244, 289
TaqI TCGA 3 cut(s) 39, 186, 225
TasI AATT 3 cut(s) 363, 425, 446
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 3 cut(s) 148, 382, 472
Tru1I TTAA 3 cut(s) 165, 420, 569
Tru9I TTAA 3 cut(s) 165, 420, 569
TscAI CASTG 1 cut(s) 217
TseI GCWGC 1 cut(s) 311
TspDTI ATGAA 1 cut(s) 10
TspRI CASTG 1 cut(s) 217
Van91I CCANNNNNTGG 1 cut(s) 448
XapI RAATTY 2 cut(s) 363, 425
XceI RCATGY 2 cut(s) 73, 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 517
XmnI GAANNNNTTC 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.