Rroxscaffold_2G00087620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018865
Physical Location & Seq
Forward (+)
9627338 .. 9628856
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087620.1

Sequence Viewer

Length: 1413 bp
ATGTCAAGTTCCAAGAAAGGTATTGAAAGTAGTAGTAGTACTACTACTGCCGCTATCAAACGAGATTTGGGTAGATTATCGGAGTCAGCCATTGGATTAATTCCAGCCTCTGCTTCTGGTCATATTCATCGGTCGAAAAGGTCCAAATCCAGGAGTAATACTAGTTTTGACGATCTCCCGGAGGATGTATTTGCCGAAATCCTTTGTCGACTTCCATGCAATAAACTTATTGTTCGATGCAAGTGTGTCTCCAAACGCTGGCAGAGCCTTGTCTCGTTTCTCACAGCGGAGGAATTACTTACCACAATGAAGATGAAAGCAGAGGAATTAATTACCACAGTGACCATGGAAGTAGAGAAGTTGCTTACCAGAATGACAACTAAAGCAGAGGATGAAGCGCTTATTACAACTGCAGACTTCGCTACCATAATGACGACAACAGCTGAGAAGTCCTTTGTTACAGTTTCGTCCAAGCCTTTAGCTCCACTGTTTAAAAACCTCATGAGAAGCTATCATTTGAAAAGAGAACCAATGGTGGTAGCAACATATAATGACTTAGTTTTGTGTCGGGATGTTGATGTTGATGTTGATGTTGATGTTGATGATGATGATGATGATGATGATGGTGCTGATGGTGGTGGTGGTGGTGATGATCCCTTAAAAAAAACGTATGATTATTACTACATCTGCAATCCATACACAGTTCAATGTGCTGCTCTCCCACGACCTCTTCGAGCCCATGAGGACGCTTACGTGCCTGTTGGATTCATATGTGATATTGACAATTCTGGGAGTAGGTGCAAAGTTGTGAGACTTCATCCAAAAGACGAAAATTCCTCCAAATTCGAAGCGGAAATCTTTTCCTCTGAAACTGGTGAATGGAGAAAGTCAATTATCTCATTCAGACAAAAAATAAGAAATTTTGTGATTAATAAGGTTACCTATCCTTACAATGGAATGTTGTATTGGAAGACTGACTACAACACTTTCGTTGGGTTTGATCCTTTCATGATCAACAGTAGTAGTGATGGTGATGATACTATTGATCATTATAAGGGTCGTATCATTGAGTTTGAGTTTGAGTTTGGGGGAATACAGATCGAGTGCGGAGGGCGTCTAAGATTCATCGACACATTCTACTTCGTTTCATATATTCTTCCTGTGTGGGAATTGGAAGAAGAACAAGATGAGAGAGGTCTTGATAGATCTGGCAAATTGTTTTTGAAACGCACGACGTATTCAGTGGATAGAGAAATAGACATGATGAGGTTCTGCGCATACGGGGTTTTTGATCCGAATGACGATAATATCTTGTACATGCAGGGACACACTAGTAGTCGATTTTTGAGATACAACATTCGTACAGGTTTACCAAATACCAACCTGGTGGCGGTGGTTACATTTTCCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

470

Amino Acids

53.39

Weight (kDa)

5.21

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 56 - 92 6.2e-07 F-box domain
b-prop_At3g26010-like PF24750 224 - 455 4e-10 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018865)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45251 FvH4_6g45260
rosa_chinensis RchiOBHm_Chr2g0163011
rosa_multiflora Rmu_sc0011038.1_g000004
rosa_roxburghii Rroxscaffold_2G00087390 Rroxscaffold_2G00087620
rosa_rugosa Rorug02G0499600
rosa_samantha Rh2AG566000 Rh2DG588700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1053
Acc16I TGCGCA 1 cut(s) 1276
AccB7I CCANNNNNTGG 1 cut(s) 258
AccI GTMKAC 1 cut(s) 208
AciI CCGC 5 cut(s) 51, 287, 851, 1107, 1391
AclWI GGATC 3 cut(s) 647, 995, 1286
AcsI RAATTY 3 cut(s) 832, 842, 919
AcyI GRCGYC 1 cut(s) 1114
AfaI GTAC 3 cut(s) 40, 1316, 1363
AfeI AGCGCT 1 cut(s) 399
AfiI CCNNNNNNNGG 4 cut(s) 150, 258, 953, 1390
AgsI TTSAA 4 cut(s) 26, 520, 707, 1225
AhlI ACTAGT 2 cut(s) 161, 1331
AjnI CCWGG 2 cut(s) 149, 1383
AjuI GAANNNNNNNTTGG 2 cut(s) 949, 981
AluBI AGCT 3 cut(s) 443, 482, 510
AluI AGCT 3 cut(s) 443, 482, 510
Alw26I GTCTC 3 cut(s) 253, 277, 805
AlwI GGATC 3 cut(s) 647, 995, 1286
AlwNI CAGNNNCTG 1 cut(s) 110
Aor51HI AGCGCT 1 cut(s) 399
ApeKI GCWGC 1 cut(s) 713
ApoI RAATTY 3 cut(s) 832, 842, 919
AseI ATTAAT 3 cut(s) 98, 329, 930
AspLEI GCGC 2 cut(s) 400, 1277
AspS9I GGNCC 1 cut(s) 141
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 3 cut(s) 659, 887, 1043
AsuII TTCGAA 1 cut(s) 846
AvaII GGWCC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 739
BarI GAAGNNNNNNTAC 2 cut(s) 962, 994
BbsI GAAGAC 1 cut(s) 977
BbvI GCAGC 1 cut(s) 700
BccI CCATC 3 cut(s) 617, 626, 1022
BcgI CGANNNNNNTGC 2 cut(s) 198, 232
BciT130I CCWGG 2 cut(s) 151, 1385
BclI TGATCA 2 cut(s) 1011, 1045
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 3 cut(s) 253, 277, 805
BcuI ACTAGT 2 cut(s) 161, 1331
BfaI CTAG 2 cut(s) 162, 1332
BfmI CTRYAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 401
BglII AGATCT 1 cut(s) 1205
BisI GCNGC 2 cut(s) 51, 714
BlsI GCNGC 2 cut(s) 52, 715
BmcAI AGTACT 1 cut(s) 40
Bme1390I CCNGG 3 cut(s) 151, 179, 1385
Bme18I GGWCC 1 cut(s) 141
BmgT120I GGNCC 1 cut(s) 141
BmrFI CCNGG 3 cut(s) 151, 179, 1385
BmsI GCATC 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 977
Bpu14I TTCGAA 1 cut(s) 846
BpuMI CCSGG 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 754
BsaHI GRCGYC 1 cut(s) 1114
BsaJI CCNNGG 1 cut(s) 345
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 258, 953, 1390
Bse1I ACTGG 1 cut(s) 877
BseBI CCWGG 2 cut(s) 151, 1385
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 4 cut(s) 190, 397, 577, 817
BseLI CCNNNNNNNGG 4 cut(s) 150, 258, 953, 1390
BseMII CTCAG 1 cut(s) 435
BseNI ACTGG 1 cut(s) 877
BseXI GCAGC 1 cut(s) 700
Bsh1285I CGRYCG 1 cut(s) 134
BsiEI CGRYCG 1 cut(s) 134
BsiSI CCGG 1 cut(s) 179
BslFI GGGAC 1 cut(s) 1338
BslI CCNNNNNNNGG 4 cut(s) 150, 258, 953, 1390
BsmAI GTCTC 3 cut(s) 253, 277, 805
BsmFI GGGAC 1 cut(s) 1338
Bsp119I TTCGAA 1 cut(s) 846
Bsp1286I GDGCHC 1 cut(s) 739
Bsp1407I TGTACA 1 cut(s) 1314
Bsp143I GATC 8 cut(s) 172, 652, 1000, 1011, 1045, 1098, 1205, 1291
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 5 cut(s) 51, 287, 851, 1107, 1391
BspCNI CTCAG 1 cut(s) 436
BspHI TCATGA 2 cut(s) 501, 1008
BspMAI CTGCAG 1 cut(s) 415
BspPI GGATC 3 cut(s) 647, 995, 1286
BspT104I TTCGAA 1 cut(s) 846
BsrGI TGTACA 1 cut(s) 1314
BsrI ACTGG 1 cut(s) 877
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 8 cut(s) 172, 652, 1000, 1011, 1045, 1098, 1205, 1291
BssNI GRCGYC 1 cut(s) 1114
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 2 cut(s) 151, 1385
Bst4CI ACNGT 5 cut(s) 340, 463, 489, 703, 1019
Bst6I CTCTTC 1 cut(s) 735
BstACI GRCGYC 1 cut(s) 1114
BstAUI TGTACA 1 cut(s) 1314
BstBAI YACGTR 1 cut(s) 754
BstBI TTCGAA 1 cut(s) 846
BstC8I GCNNGC 1 cut(s) 260
BstDEI CTNAG 3 cut(s) 444, 556, 1118
BstDSI CCRYGG 1 cut(s) 345
BstEII GGTNACC 1 cut(s) 937
BstF5I GGATG 4 cut(s) 190, 397, 577, 817
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 2 cut(s) 400, 1277
BstKTI GATC 8 cut(s) 175, 655, 1003, 1014, 1048, 1101, 1208, 1294
BstMAI GTCTC 3 cut(s) 253, 277, 805
BstMBI GATC 8 cut(s) 172, 652, 1000, 1011, 1045, 1098, 1205, 1291
BstMCI CGRYCG 1 cut(s) 134
BstMWI GCNNNNNNNGC 2 cut(s) 264, 419
BstNI CCWGG 2 cut(s) 151, 1385
BstNSI RCATGY 1 cut(s) 1321
BstPI GGTNACC 1 cut(s) 937
BstSCI CCNGG 3 cut(s) 149, 177, 1383
BstSFI CTRYAG 1 cut(s) 411
BstV1I GCAGC 1 cut(s) 700
BstV2I GAAGAC 1 cut(s) 977
BstX2I RGATCY 1 cut(s) 1205
BstXI CCANNNNNNTGG 1 cut(s) 1387
BstYI RGATCY 1 cut(s) 1205
BtgI CCRYGG 1 cut(s) 345
BtsCI GGATG 4 cut(s) 190, 397, 577, 817
BtsIMutI CAGTG 3 cut(s) 345, 485, 1248
Cac8I GCNNGC 1 cut(s) 260
CaiI CAGNNNCTG 1 cut(s) 110
CciI TCATGA 2 cut(s) 501, 1008
CfoI GCGC 2 cut(s) 400, 1277
Cfr13I GGNCC 1 cut(s) 141
CseI GACGC 2 cut(s) 755, 1103
CsiI ACCWGGT 1 cut(s) 1383
Csp6I GTAC 3 cut(s) 39, 1315, 1362
CviAII CATG 7 cut(s) 216, 346, 502, 740, 1009, 1261, 1318
CviJI RGCY 8 cut(s) 89, 107, 267, 443, 475, 482, 510, 737
CviKI_1 RGCY 8 cut(s) 89, 107, 267, 443, 475, 482, 510, 737
CviQI GTAC 3 cut(s) 39, 1315, 1362
DdeI CTNAG 3 cut(s) 444, 556, 1118
DpnI GATC 8 cut(s) 174, 654, 1002, 1013, 1047, 1100, 1207, 1293
DpnII GATC 8 cut(s) 172, 652, 1000, 1011, 1045, 1098, 1205, 1291
DraI TTTAAA 1 cut(s) 493
Eam1104I CTCTTC 1 cut(s) 735
EarI CTCTTC 1 cut(s) 735
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 739
Eco47I GGWCC 1 cut(s) 141
Eco47III AGCGCT 1 cut(s) 399
Eco91I GGTNACC 1 cut(s) 937
EcoO65I GGTNACC 1 cut(s) 937
EcoRII CCWGG 2 cut(s) 149, 1383
EcoT14I CCWWGG 1 cut(s) 345
EcoT38I GRGCYC 1 cut(s) 739
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 7 cut(s) 219, 349, 505, 743, 1012, 1264, 1321
FaqI GGGAC 1 cut(s) 1338
FatI CATG 7 cut(s) 215, 345, 501, 739, 1008, 1260, 1317
FauNDI CATATG 1 cut(s) 770
FbaI TGATCA 2 cut(s) 1011, 1045
FblI GTMKAC 1 cut(s) 208
Fnu4HI GCNGC 2 cut(s) 51, 714
FokI GGATG 4 cut(s) 197, 404, 584, 804
FriOI GRGCYC 1 cut(s) 739
Fsp4HI GCNGC 2 cut(s) 51, 714
FspBI CTAG 2 cut(s) 162, 1332
FspI TGCGCA 1 cut(s) 1276
GlaI GCGC 2 cut(s) 399, 1276
GluI GCNGC 2 cut(s) 51, 714
HaeII RGCGCY 1 cut(s) 401
HapII CCGG 1 cut(s) 179
HgaI GACGC 2 cut(s) 755, 1103
HhaI GCGC 2 cut(s) 400, 1277
Hin1I GRCGYC 1 cut(s) 1114
Hin1II CATG 7 cut(s) 219, 349, 505, 743, 1012, 1264, 1321
Hin6I GCGC 2 cut(s) 398, 1275
HinP1I GCGC 2 cut(s) 398, 1275
HincII GTYRAC 1 cut(s) 209
HindII GTYRAC 1 cut(s) 209
HinfI GANTC 3 cut(s) 83, 765, 1122
HpaII CCGG 1 cut(s) 179
HphI GGTGA 3 cut(s) 659, 887, 1043
Hpy166II GTNNAC 2 cut(s) 209, 1370
Hpy188I TCNGA 4 cut(s) 82, 868, 905, 1296
Hpy188III TCNNGA 4 cut(s) 502, 569, 1009, 1199
Hpy8I GTNNAC 2 cut(s) 209, 1370
Hpy99I CGWCG 1 cut(s) 1237
HpyCH4III ACNGT 5 cut(s) 340, 463, 489, 703, 1019
HpyCH4IV ACGT 3 cut(s) 668, 753, 1235
HpyCH4V TGCA 6 cut(s) 219, 240, 413, 690, 801, 1321
HpyF10VI GCNNNNNNNGC 2 cut(s) 264, 419
HpyF3I CTNAG 3 cut(s) 444, 556, 1118
HpySE526I ACGT 3 cut(s) 668, 753, 1235
Hsp92I GRCGYC 1 cut(s) 1114
Hsp92II CATG 7 cut(s) 219, 349, 505, 743, 1012, 1264, 1321
HspAI GCGC 2 cut(s) 398, 1275
Ksp22I TGATCA 2 cut(s) 1011, 1045
Kzo9I GATC 8 cut(s) 172, 652, 1000, 1011, 1045, 1098, 1205, 1291
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 1 cut(s) 700
LweI GCATC 1 cut(s) 227
MabI ACCWGGT 1 cut(s) 1383
MaeI CTAG 2 cut(s) 162, 1332
MaeII ACGT 3 cut(s) 668, 753, 1235
MaeIII GTNAC 4 cut(s) 340, 457, 937, 1396
MalI GATC 8 cut(s) 174, 654, 1002, 1013, 1047, 1100, 1207, 1293
MboI GATC 8 cut(s) 172, 652, 1000, 1011, 1045, 1098, 1205, 1291
MboII GAAGA 6 cut(s) 322, 722, 982, 1148, 1187, 1190
MflI RGATCY 1 cut(s) 1205
MhlI GDGCHC 1 cut(s) 739
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 742
MseI TTAA 5 cut(s) 98, 329, 492, 659, 930
MspA1I CMGCKG 2 cut(s) 287, 443
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 3 cut(s) 151, 179, 1385
MvaI CCWGG 2 cut(s) 151, 1385
MwoI GCNNNNNNNGC 2 cut(s) 264, 419
NciI CCSGG 1 cut(s) 179
NcoI CCATGG 1 cut(s) 345
NdeI CATATG 1 cut(s) 770
NdeII GATC 8 cut(s) 172, 652, 1000, 1011, 1045, 1098, 1205, 1291
NlaIII CATG 7 cut(s) 219, 349, 505, 743, 1012, 1264, 1321
NmuCI GTSAC 1 cut(s) 340
NsbI TGCGCA 1 cut(s) 1276
NspI RCATGY 1 cut(s) 1321
NspV TTCGAA 1 cut(s) 846
PagI TCATGA 2 cut(s) 501, 1008
PcsI WCGNNNNNNNCGW 1 cut(s) 730
PfeI GAWTC 2 cut(s) 765, 1122
PflMI CCANNNNNTGG 1 cut(s) 258
PfoI TCCNGGA 2 cut(s) 149, 177
PkrI GCNGC 2 cut(s) 52, 715
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
Ppu21I YACGTR 1 cut(s) 754
PshBI ATTAAT 3 cut(s) 98, 329, 930
PsiI TTATAA 1 cut(s) 1053
Psp6I CCWGG 2 cut(s) 149, 1383
PspEI GGTNACC 1 cut(s) 937
PspGI CCWGG 2 cut(s) 149, 1383
PspPI GGNCC 1 cut(s) 141
PstI CTGCAG 1 cut(s) 415
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 1 cut(s) 1205
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 3 cut(s) 40, 1316, 1363
RsaNI GTAC 3 cut(s) 39, 1315, 1362
SalI GTCGAC 1 cut(s) 207
SaqAI TTAA 5 cut(s) 98, 329, 492, 659, 930
SatI GCNGC 2 cut(s) 51, 714
Sau3AI GATC 8 cut(s) 172, 652, 1000, 1011, 1045, 1098, 1205, 1291
Sau96I GGNCC 1 cut(s) 141
ScaI AGTACT 1 cut(s) 40
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 3 cut(s) 151, 179, 1385
SduI GDGCHC 1 cut(s) 739
SexAI ACCWGGT 1 cut(s) 1383
SfaNI GCATC 1 cut(s) 227
SfcI CTRYAG 1 cut(s) 411
SfuI TTCGAA 1 cut(s) 846
SinI GGWCC 1 cut(s) 141
SpeI ACTAGT 2 cut(s) 161, 1331
SsiI CCGC 5 cut(s) 51, 287, 851, 1107, 1391
SspMI CTAG 2 cut(s) 162, 1332
StyD4I CCNGG 3 cut(s) 149, 177, 1383
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 5 cut(s) 340, 463, 489, 703, 1019
TaiI ACGT 3 cut(s) 671, 756, 1238
TaqI TCGA 8 cut(s) 134, 208, 235, 733, 846, 1101, 1128, 1339
TaqII GACCGA 1 cut(s) 120
TatI WGTACW 2 cut(s) 38, 1314
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 2 cut(s) 765, 1122
Tru1I TTAA 5 cut(s) 98, 329, 492, 659, 930
Tru9I TTAA 5 cut(s) 98, 329, 492, 659, 930
TscAI CASTG 3 cut(s) 345, 492, 1248
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 1 cut(s) 713
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 9 cut(s) 116, 323, 329, 408, 757, 806, 997, 1114, 1137
TspRI CASTG 3 cut(s) 345, 492, 1248
Van91I CCANNNNNTGG 1 cut(s) 258
VpaK11BI GGWCC 1 cut(s) 141
VspI ATTAAT 3 cut(s) 98, 329, 930
XapI RAATTY 3 cut(s) 832, 842, 919
XceI RCATGY 1 cut(s) 1321
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 208
XspI CTAG 2 cut(s) 162, 1332
ZrmI AGTACT 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.