Rh2AG566000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018865
Physical Location & Seq
Reverse (-)
79730160 .. 79731007
848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG566000.1

Sequence Viewer

Length: 840 bp
ATGTATGATTATTACTACATCTGCAATCCATACACAGTTCAATGTTTTGCTCTCCCACGACCTCTTCGAGCCTATGAGTACTCTTACGTGCCTGTTGGATTCATATGTGATCTTGACAATTCTGGGTGTAGGTGCAAAGTTGTGAGATTTCATCCAAAAGATAATGAAAATTCCTCCAAATTCGAAGTGGAAATCTTTTCCTCCGAAACTGGTGAATGGAGAAAGTCAATTATCTCATTCAAACAAAAAATATCTTCTTGTGTGATGGATAAGGTTACCTATCCTTACAATGGAATGTTGTATTGGAAGACTGACTACAACACTTTCGTTGGGTTTGATCCTTTCATGATCAACAGTAGTAGTGATGGTGATGATACTATTGATCATTATAAAGGTCGTATCATTGAGTTTGAGTTTGGGAGAATACAGATCGAGTGTGTAGGCATGTGCGGAGGGCGTTTAAGATTCATCGACAGATTCGACTTCGTTTCATATATTCTTCCTGTGTGGGAATTGGAAGAAGAACAAGATGAGAGACGTCTTGATAGATCTGACAAGTTGTTTTTGAAACGCACGACGTATTCACTGGATAGGGAAATGATTGATACACACCTAGACATGATGAGGTTCTATGCATACCGGGTTTTTGATCCGAATGACGATAATATCTTGTACATGCAGGGACACACTAGTAGTCGATTTTGGAGATACAACATTCGTACAAGAAGGTGGTCAAAGATGTGTCATGCGCCCGATGTCAACATTTACCAACTAGGTGGCTGTGGTTCCATTTTCCCATTGACAGTGCTCCCGTGGTGGCCGACACCGGTTCCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

279

Amino Acids

33.32

Weight (kDa)

6.0

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 3 - 246 3.4e-15 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018865)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45251 FvH4_6g45260
rosa_chinensis RchiOBHm_Chr2g0163011
rosa_multiflora Rmu_sc0011038.1_g000004
rosa_roxburghii Rroxscaffold_2G00087390 Rroxscaffold_2G00087620
rosa_rugosa Rorug02G0499600
rosa_samantha Rh2AG566000 Rh2DG588700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 390
AatII GACGTC 1 cut(s) 541
AciI CCGC 1 cut(s) 450
AclWI GGATC 2 cut(s) 332, 644
AcoI YGGCCR 1 cut(s) 818
AcsI RAATTY 2 cut(s) 169, 179
AcyI GRCGYC 1 cut(s) 538
AfaI GTAC 3 cut(s) 80, 674, 721
AfiI CCNNNNNNNGG 1 cut(s) 290
AgeI ACCGGT 1 cut(s) 826
AgsI TTSAA 3 cut(s) 41, 241, 568
AhlI ACTAGT 1 cut(s) 689
AjuI GAANNNNNNNTTGG 2 cut(s) 286, 318
Alw21I GWGCWC 1 cut(s) 810
Alw26I GTCTC 1 cut(s) 529
AlwI GGATC 2 cut(s) 332, 644
AoxI GGCC 1 cut(s) 818
ApoI RAATTY 2 cut(s) 169, 179
AsiGI ACCGGT 1 cut(s) 826
AspLEI GCGC 1 cut(s) 751
AsuC2I CCSGG 1 cut(s) 641
AsuHPI GGTGA 2 cut(s) 224, 380
AsuII TTCGAA 1 cut(s) 183
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
BbsI GAAGAC 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 810
BccI CCATC 2 cut(s) 259, 359
BclI TGATCA 2 cut(s) 348, 382
BcnI CCSGG 1 cut(s) 641
BcoDI GTCTC 1 cut(s) 529
BcuI ACTAGT 1 cut(s) 689
BfaI CTAG 3 cut(s) 614, 690, 773
BglII AGATCT 1 cut(s) 548
BmcAI AGTACT 1 cut(s) 80
Bme1390I CCNGG 1 cut(s) 641
BmiI GGNNCC 2 cut(s) 787, 831
BmrFI CCNGG 1 cut(s) 641
BpiI GAAGAC 1 cut(s) 314
Bpu14I TTCGAA 1 cut(s) 183
BpuMI CCSGG 1 cut(s) 641
BsaAI YACGTR 1 cut(s) 88
BsaHI GRCGYC 1 cut(s) 538
BsaJI CCNNGG 1 cut(s) 812
BsaWI WCCGGW 1 cut(s) 826
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse118I RCCGGY 1 cut(s) 826
Bse1I ACTGG 2 cut(s) 214, 591
BseDI CCNNGG 1 cut(s) 812
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 1 cut(s) 290
BseNI ACTGG 2 cut(s) 214, 591
BshFI GGCC 1 cut(s) 820
BshTI ACCGGT 1 cut(s) 826
BsiHKAI GWGCWC 1 cut(s) 810
BsiSI CCGG 2 cut(s) 640, 827
BslFI GGGAC 1 cut(s) 696
BslI CCNNNNNNNGG 1 cut(s) 290
BsmAI GTCTC 1 cut(s) 529
BsmBI CGTCTC 1 cut(s) 529
BsmFI GGGAC 1 cut(s) 696
BsnI GGCC 1 cut(s) 820
Bsp119I TTCGAA 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 810
Bsp1407I TGTACA 1 cut(s) 672
Bsp143I GATC 7 cut(s) 109, 337, 348, 382, 429, 548, 649
BspACI CCGC 1 cut(s) 450
BspANI GGCC 1 cut(s) 820
BspHI TCATGA 1 cut(s) 345
BspLI GGNNCC 2 cut(s) 787, 831
BspPI GGATC 2 cut(s) 332, 644
BspT104I TTCGAA 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 826
BsrGI TGTACA 1 cut(s) 672
BsrI ACTGG 2 cut(s) 214, 591
BssAI RCCGGY 1 cut(s) 826
BssECI CCNNGG 1 cut(s) 812
BssMI GATC 7 cut(s) 109, 337, 348, 382, 429, 548, 649
BssNI GRCGYC 1 cut(s) 538
Bst4CI ACNGT 3 cut(s) 37, 356, 805
Bst6I CTCTTC 1 cut(s) 69
BstACI GRCGYC 1 cut(s) 538
BstAUI TGTACA 1 cut(s) 672
BstBAI YACGTR 1 cut(s) 88
BstBI TTCGAA 1 cut(s) 183
BstDSI CCRYGG 1 cut(s) 812
BstEII GGTNACC 1 cut(s) 274
BstF5I GGATG 1 cut(s) 151
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 7 cut(s) 112, 340, 351, 385, 432, 551, 652
BstMAI GTCTC 1 cut(s) 529
BstMBI GATC 7 cut(s) 109, 337, 348, 382, 429, 548, 649
BstNSI RCATGY 2 cut(s) 448, 679
BstPI GGTNACC 1 cut(s) 274
BstSCI CCNGG 1 cut(s) 639
BstV2I GAAGAC 1 cut(s) 314
BstX2I RGATCY 1 cut(s) 548
BstXI CCANNNNNNTGG 1 cut(s) 776
BstYI RGATCY 1 cut(s) 548
BsuRI GGCC 1 cut(s) 820
BtgI CCRYGG 1 cut(s) 812
BtsCI GGATG 1 cut(s) 151
BtsIMutI CAGTG 2 cut(s) 584, 810
CciI TCATGA 1 cut(s) 345
CfoI GCGC 1 cut(s) 751
Cfr10I RCCGGY 1 cut(s) 826
Csp6I GTAC 3 cut(s) 79, 673, 720
CspAI ACCGGT 1 cut(s) 826
CviAII CATG 5 cut(s) 346, 445, 619, 676, 746
CviJI RGCY 3 cut(s) 71, 780, 820
CviKI_1 RGCY 3 cut(s) 71, 780, 820
CviQI GTAC 3 cut(s) 79, 673, 720
DpnI GATC 7 cut(s) 111, 339, 350, 384, 431, 550, 651
DpnII GATC 7 cut(s) 109, 337, 348, 382, 429, 548, 649
EaeI YGGCCR 1 cut(s) 818
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco91I GGTNACC 1 cut(s) 274
EcoO65I GGTNACC 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 637
Esp3I CGTCTC 1 cut(s) 529
FaeI CATG 5 cut(s) 349, 448, 622, 679, 749
FaqI GGGAC 1 cut(s) 696
FatI CATG 5 cut(s) 345, 444, 618, 675, 745
FauNDI CATATG 1 cut(s) 104
FbaI TGATCA 2 cut(s) 348, 382
FokI GGATG 1 cut(s) 138
FspBI CTAG 3 cut(s) 614, 690, 773
GlaI GCGC 1 cut(s) 750
HaeIII GGCC 1 cut(s) 820
HapII CCGG 2 cut(s) 640, 827
HhaI GCGC 1 cut(s) 751
Hin1I GRCGYC 1 cut(s) 538
Hin1II CATG 5 cut(s) 349, 448, 622, 679, 749
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HinfI GANTC 3 cut(s) 99, 465, 477
HpaII CCGG 2 cut(s) 640, 827
HphI GGTGA 2 cut(s) 224, 380
Hpy166II GTNNAC 1 cut(s) 760
Hpy188I TCNGA 3 cut(s) 205, 553, 654
Hpy188III TCNNGA 3 cut(s) 113, 346, 542
Hpy8I GTNNAC 1 cut(s) 760
Hpy99I CGWCG 1 cut(s) 580
HpyAV CCTTC 1 cut(s) 720
HpyCH4III ACNGT 3 cut(s) 37, 356, 805
HpyCH4IV ACGT 3 cut(s) 87, 538, 578
HpyCH4V TGCA 4 cut(s) 24, 135, 635, 679
HpySE526I ACGT 3 cut(s) 87, 538, 578
Hsp92I GRCGYC 1 cut(s) 538
Hsp92II CATG 5 cut(s) 349, 448, 622, 679, 749
HspAI GCGC 1 cut(s) 749
Ksp22I TGATCA 2 cut(s) 348, 382
Kzo9I GATC 7 cut(s) 109, 337, 348, 382, 429, 548, 649
LmnI GCTCC 1 cut(s) 813
LpnPI CCDG 7 cut(s) 105, 108, 195, 516, 572, 653, 665
MaeI CTAG 3 cut(s) 614, 690, 773
MaeII ACGT 3 cut(s) 87, 538, 578
MaeIII GTNAC 1 cut(s) 274
MalI GATC 7 cut(s) 111, 339, 350, 384, 431, 550, 651
MboI GATC 7 cut(s) 109, 337, 348, 382, 429, 548, 649
MboII GAAGA 6 cut(s) 56, 246, 319, 491, 530, 533
MflI RGATCY 1 cut(s) 548
MhlI GDGCHC 1 cut(s) 810
MluCI AATT 5 cut(s) 118, 169, 179, 228, 512
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 5 cut(s) 72, 184, 211, 446, 618
Mph1103I ATGCAT 1 cut(s) 637
MseI TTAA 1 cut(s) 461
MspI CCGG 2 cut(s) 640, 827
MspR9I CCNGG 1 cut(s) 641
NciI CCSGG 1 cut(s) 641
NdeI CATATG 1 cut(s) 104
NdeII GATC 7 cut(s) 109, 337, 348, 382, 429, 548, 649
NlaIII CATG 5 cut(s) 349, 448, 622, 679, 749
NlaIV GGNNCC 2 cut(s) 787, 831
NsiI ATGCAT 1 cut(s) 637
NspI RCATGY 2 cut(s) 448, 679
NspV TTCGAA 1 cut(s) 183
PagI TCATGA 1 cut(s) 345
PcsI WCGNNNNNNNCGW 2 cut(s) 64, 477
PfeI GAWTC 3 cut(s) 99, 465, 477
PinAI ACCGGT 1 cut(s) 826
Ppu21I YACGTR 1 cut(s) 88
PsiI TTATAA 1 cut(s) 390
PspEI GGTNACC 1 cut(s) 274
PspN4I GGNNCC 2 cut(s) 787, 831
PsuI RGATCY 1 cut(s) 548
RsaI GTAC 3 cut(s) 80, 674, 721
RsaNI GTAC 3 cut(s) 79, 673, 720
SaqAI TTAA 1 cut(s) 461
Sau3AI GATC 7 cut(s) 109, 337, 348, 382, 429, 548, 649
ScaI AGTACT 1 cut(s) 80
ScrFI CCNGG 1 cut(s) 641
SduI GDGCHC 1 cut(s) 810
SfuI TTCGAA 1 cut(s) 183
SpeI ACTAGT 1 cut(s) 689
Sse9I AATT 5 cut(s) 118, 169, 179, 228, 512
SsiI CCGC 1 cut(s) 450
SspMI CTAG 3 cut(s) 614, 690, 773
StyD4I CCNGG 1 cut(s) 639
TaaI ACNGT 3 cut(s) 37, 356, 805
TaiI ACGT 3 cut(s) 90, 541, 581
TaqI TCGA 6 cut(s) 67, 183, 432, 471, 480, 697
TasI AATT 5 cut(s) 118, 169, 179, 228, 512
TatI WGTACW 2 cut(s) 78, 672
TfiI GAWTC 3 cut(s) 99, 465, 477
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TscAI CASTG 2 cut(s) 591, 810
TspDTI ATGAA 6 cut(s) 91, 140, 180, 334, 457, 480
TspRI CASTG 2 cut(s) 591, 810
XapI RAATTY 2 cut(s) 169, 179
XceI RCATGY 2 cut(s) 448, 679
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XspI CTAG 3 cut(s) 614, 690, 773
ZraI GACGTC 1 cut(s) 539
ZrmI AGTACT 1 cut(s) 80
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.