Rh2DG588700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018865
Physical Location & Seq
Reverse (-)
82174947 .. 82176497
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG588700.1

Sequence Viewer

Length: 1551 bp
ATGTCAAGTTCCAAGAAAGGTATTGAAAGTAGTAGTACTACTACTGCCGCTATCAAACGAGATTTGGGTAGTTTATCGGCCTCTGCTTCTGGTCATATTCATCGGTCGAATAGGTCCAAATCCAGGAGTAATACTAGTTTTGACGATCTCCCTGAGGATGTATTTGCCGAAATCCTTTGTCGACTTCCTTGCAATAAACTTCTTGTTCGATGCACGTGTGTGTCCAAACGCTGGCAGAGCCTTGTCTCGTTTCTCATATGTGATCCTAAGTTTATTGGACGGTTTATATGCCTCAAGCATGATGGTAAAACACCAATAATTCGTAGTCTACTGAATTGGGAAGGAAAAGAATTTCGTGTCACCAAAGCGGAGGAATTACTTACCACAATGAAGATGAAAGCAGAGGAATTGATTACCGCGGTGACTATGAAAGTAGAAAAGTTGCTGACCAGAATGACAACTAAAGCAGAGGATGAAGCGTATATTACAACAGCAGACTTCGCTACCATAATGACCACAACCGCTGAGAAATCATTTGTTACAGTTTCGTCCAAGCCTTTAGCTCCACTGTTTAAAAACCTCATGAGAAGCTATCATTTGAAAGAAAAACCAATGGTGTTAGCAACATATAATGACTTGGTTTTGTGTCGGGATCTTCATGTTGATGTTGATGACGATGCTCCCTTCAAAAAAGCGTATGAGTATTACTACATCTGCAATCCATACACATTTCAATGTGTTGCTCTCCCACGACCTCTTCGCGCCCATGATCAGTACGGTCACGTGCCTGTTGGATTCATATGTGATCTTGACAATTCTGGGAGTAGGTGCAAAGTTGTGAGATTTCATCCAATAGATAAAGAAAATTCCTCCAAATTAGAAGTAGAAATCTTTTCCTCCGAAACTGGTGAATGGAGAAGGTCAATTATCTCATTCAGACAAAAAGTATCTTCTTGTCATATGAATATGGTTACCTATCCTTACAATGGAATGTTGTATTATTGGAACTCTGACTACAACAATTTCATTGGGTTTGATCCTTTCATGATCAACAGTAGTAGCGATGGTGATGATACTATTGAACGTTATAAAGGTCGTATCATTGAGTTTGAGTTTGGTGGGGAGAGCTCTAAGATCGAGTGTGTAGGCATGTGCGGAGGGCGTCTAAGATTCATCGGCGGATTCGACCTCATTTCATGTATTCTTCCTGTGTGGGAGCTGAAAGAAGAACAAGCTGAGAGAGCTCTCGATAGATCTGGCAAATTGTTTTTGAAACGCAAGACGTATTCACTGGATAGAGAAATGATTGATGCACACCAATACATGATGAGGTTCTGTGCAGACCGGTTTTTTGATCCGAATGACGAGAATATCTTGTGCATGCATACACACGGACATACTGGTAGTGAATTTTTGAGATACAACATTCGTACAAGAAAGTGGTCAGTGATGTGTCATGCGCCCGATGTCCGAATTTACAAACTCAATGGCACTGGTTACATTTTCCCATTGACAGTGCTCCCGTGGTGGCCGACACCGGTTCCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

516

Amino Acids

59.34

Weight (kDa)

8.5

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 47 - 83 1.6e-06 F-box domain
b-prop_At3g26010-like PF24750 203 - 483 2.1e-13 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018865)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45251 FvH4_6g45260
rosa_chinensis RchiOBHm_Chr2g0163011
rosa_multiflora Rmu_sc0011038.1_g000004
rosa_roxburghii Rroxscaffold_2G00087390 Rroxscaffold_2G00087620
rosa_rugosa Rorug02G0499600
rosa_samantha Rh2AG566000 Rh2DG588700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1089
AccB7I CCANNNNNTGG 1 cut(s) 231
AccI GTMKAC 2 cut(s) 181, 328
AccII CGCG 2 cut(s) 419, 762
AciI CCGC 7 cut(s) 48, 368, 417, 419, 522, 1155, 1179
AclI AACGTT 1 cut(s) 1084
AclWI GGATC 4 cut(s) 257, 660, 1031, 1349
AcoI YGGCCR 1 cut(s) 1529
AcsI RAATTY 4 cut(s) 350, 865, 1409, 1473
AcvI CACGTG 2 cut(s) 216, 784
AcyI GRCGYC 1 cut(s) 1162
AfaI GTAC 3 cut(s) 37, 776, 1432
AfiI CCNNNNNNNGG 3 cut(s) 123, 231, 986
AflIII ACRYGT 1 cut(s) 215
AgeI ACCGGT 2 cut(s) 1344, 1537
AgsI TTSAA 6 cut(s) 26, 601, 688, 734, 1082, 1273
AhlI ACTAGT 1 cut(s) 134
AjnI CCWGG 1 cut(s) 122
AleI CACNNNNGTG 1 cut(s) 218
AluBI AGCT 6 cut(s) 563, 591, 1128, 1219, 1235, 1244
AluI AGCT 6 cut(s) 563, 591, 1128, 1219, 1235, 1244
Alw21I GWGCWC 3 cut(s) 1130, 1246, 1521
Alw26I GTCTC 1 cut(s) 250
AlwI GGATC 4 cut(s) 257, 660, 1031, 1349
AoxI GGCC 2 cut(s) 78, 1529
ApoI RAATTY 4 cut(s) 350, 865, 1409, 1473
ArsI GACNNNNNNTTYG 2 cut(s) 1334, 1366
AsiGI ACCGGT 2 cut(s) 1344, 1537
AspLEI GCGC 2 cut(s) 764, 1462
AspS9I GGNCC 1 cut(s) 114
AsuHPI GGTGA 4 cut(s) 352, 433, 920, 1079
AvaII GGWCC 1 cut(s) 114
AxyI CCTNAGG 1 cut(s) 153
BanII GRGCYC 2 cut(s) 1130, 1246
BbrPI CACGTG 2 cut(s) 216, 784
Bbv12I GWGCWC 3 cut(s) 1130, 1246, 1521
BccI CCATC 2 cut(s) 296, 1058
BcgI CGANNNNNNTGC 2 cut(s) 171, 205
BciT130I CCWGG 1 cut(s) 124
BclI TGATCA 2 cut(s) 769, 1047
BcoDI GTCTC 1 cut(s) 250
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 1 cut(s) 135
BglII AGATCT 1 cut(s) 1253
BisI GCNGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 49
BmcAI AGTACT 1 cut(s) 37
Bme1390I CCNGG 1 cut(s) 124
Bme18I GGWCC 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 1 cut(s) 1542
BmrFI CCNGG 1 cut(s) 124
BmsI GCATC 3 cut(s) 200, 667, 1300
BpuEI CTTGAG 1 cut(s) 278
BsaAI YACGTR 2 cut(s) 216, 784
BsaHI GRCGYC 1 cut(s) 1162
BsaJI CCNNGG 2 cut(s) 417, 1523
BsaWI WCCGGW 2 cut(s) 1344, 1537
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 231, 986
Bse118I RCCGGY 2 cut(s) 1344, 1537
Bse1I ACTGG 4 cut(s) 910, 1296, 1405, 1498
Bse21I CCTNAGG 1 cut(s) 153
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 2 cut(s) 417, 1523
BseGI GGATG 3 cut(s) 163, 478, 847
BseLI CCNNNNNNNGG 3 cut(s) 123, 231, 986
BseMII CTCAG 3 cut(s) 144, 516, 1227
BseNI ACTGG 4 cut(s) 910, 1296, 1405, 1498
BsgI GTGCAG 1 cut(s) 1359
Bsh1236I CGCG 2 cut(s) 419, 762
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 2 cut(s) 80, 1531
BshTI ACCGGT 2 cut(s) 1344, 1537
BsiEI CGRYCG 1 cut(s) 107
BsiHKAI GWGCWC 3 cut(s) 1130, 1246, 1521
BsiSI CCGG 2 cut(s) 1345, 1538
BslI CCNNNNNNNGG 3 cut(s) 123, 231, 986
BsmAI GTCTC 1 cut(s) 250
BsnI GGCC 2 cut(s) 80, 1531
Bsp1286I GDGCHC 3 cut(s) 1130, 1246, 1521
BspACI CCGC 7 cut(s) 48, 368, 417, 419, 522, 1155, 1179
BspANI GGCC 2 cut(s) 80, 1531
BspCNI CTCAG 3 cut(s) 145, 517, 1228
BspFNI CGCG 2 cut(s) 419, 762
BspHI TCATGA 2 cut(s) 582, 1044
BspLI GGNNCC 1 cut(s) 1542
BspPI GGATC 4 cut(s) 257, 660, 1031, 1349
BsrFI RCCGGY 2 cut(s) 1344, 1537
BsrI ACTGG 4 cut(s) 910, 1296, 1405, 1498
BssAI RCCGGY 2 cut(s) 1344, 1537
BssECI CCNNGG 2 cut(s) 417, 1523
BssNI GRCGYC 1 cut(s) 1162
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 6 cut(s) 282, 544, 570, 779, 1055, 1516
Bst6I CTCTTC 1 cut(s) 762
BstACI GRCGYC 1 cut(s) 1162
BstBAI YACGTR 2 cut(s) 216, 784
BstC8I GCNNGC 2 cut(s) 233, 1382
BstDEI CTNAG 6 cut(s) 153, 267, 525, 1131, 1166, 1236
BstDSI CCRYGG 2 cut(s) 417, 1523
BstEII GGTNACC 1 cut(s) 970
BstF5I GGATG 3 cut(s) 163, 478, 847
BstFNI CGCG 2 cut(s) 419, 762
BstHHI GCGC 2 cut(s) 764, 1462
BstMAI GTCTC 1 cut(s) 250
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 3 cut(s) 237, 500, 1241
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 2 cut(s) 1153, 1384
BstPI GGTNACC 1 cut(s) 970
BstSCI CCNGG 1 cut(s) 122
BstUI CGCG 2 cut(s) 419, 762
BstX2I RGATCY 2 cut(s) 652, 1253
BstYI RGATCY 2 cut(s) 652, 1253
Bsu36I CCTNAGG 1 cut(s) 153
BsuRI GGCC 2 cut(s) 80, 1531
BtgI CCRYGG 2 cut(s) 417, 1523
BtgZI GCGATG 1 cut(s) 1077
BtsCI GGATG 3 cut(s) 163, 478, 847
BtsIMutI CAGTG 5 cut(s) 566, 1289, 1452, 1491, 1521
Cac8I GCNNGC 2 cut(s) 233, 1382
CciI TCATGA 2 cut(s) 582, 1044
CfoI GCGC 2 cut(s) 764, 1462
Cfr10I RCCGGY 2 cut(s) 1344, 1537
Cfr13I GGNCC 1 cut(s) 114
Cfr42I CCGCGG 1 cut(s) 420
CseI GACGC 1 cut(s) 1151
Csp6I GTAC 3 cut(s) 36, 775, 1431
CspAI ACCGGT 2 cut(s) 1344, 1537
CviQI GTAC 3 cut(s) 36, 775, 1431
DdeI CTNAG 6 cut(s) 153, 267, 525, 1131, 1166, 1236
DraI TTTAAA 1 cut(s) 574
EaeI YGGCCR 1 cut(s) 1529
Eam1104I CTCTTC 1 cut(s) 762
EarI CTCTTC 1 cut(s) 762
EciI GGCGGA 1 cut(s) 1194
Ecl136II GAGCTC 2 cut(s) 1128, 1244
Eco24I GRGCYC 2 cut(s) 1130, 1246
Eco47I GGWCC 1 cut(s) 114
Eco53kI GAGCTC 2 cut(s) 1128, 1244
Eco72I CACGTG 2 cut(s) 216, 784
Eco81I CCTNAGG 1 cut(s) 153
Eco91I GGTNACC 1 cut(s) 970
EcoICRI GAGCTC 2 cut(s) 1128, 1244
EcoO65I GGTNACC 1 cut(s) 970
EcoRII CCWGG 1 cut(s) 122
EcoT22I ATGCAT 1 cut(s) 1386
EcoT38I GRGCYC 2 cut(s) 1130, 1246
FauNDI CATATG 3 cut(s) 257, 800, 960
FbaI TGATCA 2 cut(s) 769, 1047
FblI GTMKAC 2 cut(s) 181, 328
Fnu4HI GCNGC 1 cut(s) 48
FokI GGATG 3 cut(s) 170, 485, 834
FriOI GRGCYC 2 cut(s) 1130, 1246
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 1 cut(s) 135
GlaI GCGC 2 cut(s) 763, 1461
GluI GCNGC 1 cut(s) 48
HaeIII GGCC 2 cut(s) 80, 1531
HapII CCGG 2 cut(s) 1345, 1538
HgaI GACGC 1 cut(s) 1151
HhaI GCGC 2 cut(s) 764, 1462
Hin1I GRCGYC 1 cut(s) 1162
Hin6I GCGC 2 cut(s) 762, 1460
HinP1I GCGC 2 cut(s) 762, 1460
HincII GTYRAC 1 cut(s) 182
HindII GTYRAC 1 cut(s) 182
HinfI GANTC 3 cut(s) 795, 1170, 1182
HpaII CCGG 2 cut(s) 1345, 1538
HphI GGTGA 4 cut(s) 352, 433, 920, 1079
Hpy166II GTNNAC 2 cut(s) 182, 329
Hpy188I TCNGA 5 cut(s) 901, 938, 1012, 1359, 1472
Hpy188III TCNNGA 5 cut(s) 583, 650, 809, 1045, 1247
Hpy8I GTNNAC 2 cut(s) 182, 329
HpyAV CCTTC 3 cut(s) 335, 694, 912
HpyCH4III ACNGT 6 cut(s) 282, 544, 570, 779, 1055, 1516
HpyCH4IV ACGT 4 cut(s) 215, 783, 1084, 1283
HpyCH4V TGCA 8 cut(s) 192, 213, 717, 831, 1313, 1340, 1380, 1384
HpyF10VI GCNNNNNNNGC 3 cut(s) 237, 500, 1241
HpyF3I CTNAG 6 cut(s) 153, 267, 525, 1131, 1166, 1236
HpySE526I ACGT 4 cut(s) 215, 783, 1084, 1283
Hsp92I GRCGYC 1 cut(s) 1162
HspAI GCGC 2 cut(s) 762, 1460
Ksp22I TGATCA 2 cut(s) 769, 1047
KspI CCGCGG 1 cut(s) 420
LmnI GCTCC 4 cut(s) 568, 685, 1216, 1524
LweI GCATC 3 cut(s) 200, 667, 1300
MaeI CTAG 1 cut(s) 135
MaeII ACGT 4 cut(s) 215, 783, 1084, 1283
MaeIII GTNAC 6 cut(s) 358, 421, 538, 779, 970, 1496
MboII GAAGA 6 cut(s) 403, 647, 749, 942, 1196, 1238
MflI RGATCY 2 cut(s) 652, 1253
MhlI GDGCHC 3 cut(s) 1130, 1246, 1521
MmeI TCCRAC 1 cut(s) 772
Mph1103I ATGCAT 1 cut(s) 1386
MseI TTAA 1 cut(s) 573
MslI CAYNNNNRTG 3 cut(s) 218, 663, 733
MspA1I CMGCKG 2 cut(s) 419, 524
MspI CCGG 2 cut(s) 1345, 1538
MspR9I CCNGG 1 cut(s) 124
MvaI CCWGG 1 cut(s) 124
MvnI CGCG 2 cut(s) 419, 762
MwoI GCNNNNNNNGC 3 cut(s) 237, 500, 1241
NdeI CATATG 3 cut(s) 257, 800, 960
NlaIV GGNNCC 1 cut(s) 1542
NmuCI GTSAC 3 cut(s) 358, 421, 779
NsiI ATGCAT 1 cut(s) 1386
NspI RCATGY 2 cut(s) 1153, 1384
OliI CACNNNNGTG 1 cut(s) 218
PaeI GCATGC 1 cut(s) 1384
PagI TCATGA 2 cut(s) 582, 1044
PcsI WCGNNNNNNNCGW 1 cut(s) 1182
PfeI GAWTC 3 cut(s) 795, 1170, 1182
PflMI CCANNNNNTGG 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 122
PinAI ACCGGT 2 cut(s) 1344, 1537
PkrI GCNGC 1 cut(s) 49
PmaCI CACGTG 2 cut(s) 216, 784
PmlI CACGTG 2 cut(s) 216, 784
Ppu21I YACGTR 2 cut(s) 216, 784
PsiI TTATAA 1 cut(s) 1089
Psp124BI GAGCTC 2 cut(s) 1130, 1246
Psp1406I AACGTT 1 cut(s) 1084
Psp6I CCWGG 1 cut(s) 122
PspCI CACGTG 2 cut(s) 216, 784
PspEI GGTNACC 1 cut(s) 970
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 1 cut(s) 1542
PspPI GGNCC 1 cut(s) 114
PsrI GAACNNNNNNTAC 2 cut(s) 998, 1030
PsuI RGATCY 2 cut(s) 652, 1253
RsaI GTAC 3 cut(s) 37, 776, 1432
RsaNI GTAC 3 cut(s) 36, 775, 1431
RseI CAYNNNNRTG 3 cut(s) 218, 663, 733
SacI GAGCTC 2 cut(s) 1130, 1246
SacII CCGCGG 1 cut(s) 420
SalI GTCGAC 1 cut(s) 180
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 1 cut(s) 48
Sau96I GGNCC 1 cut(s) 114
ScaI AGTACT 1 cut(s) 37
ScrFI CCNGG 1 cut(s) 124
SduI GDGCHC 3 cut(s) 1130, 1246, 1521
SfaNI GCATC 3 cut(s) 200, 667, 1300
Sfr303I CCGCGG 1 cut(s) 420
SgrBI CCGCGG 1 cut(s) 420
SinI GGWCC 1 cut(s) 114
SmiMI CAYNNNNRTG 3 cut(s) 218, 663, 733
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SpeI ACTAGT 1 cut(s) 134
SphI GCATGC 1 cut(s) 1384
SsiI CCGC 7 cut(s) 48, 368, 417, 419, 522, 1155, 1179
SspMI CTAG 1 cut(s) 135
SstI GAGCTC 2 cut(s) 1130, 1246
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 6 cut(s) 282, 544, 570, 779, 1055, 1516
TaiI ACGT 4 cut(s) 218, 786, 1087, 1286
TaqI TCGA 6 cut(s) 107, 181, 208, 1137, 1185, 1248
TaqII GACCGA 1 cut(s) 93
TatI WGTACW 1 cut(s) 35
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 3 cut(s) 795, 1170, 1182
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TscAI CASTG 5 cut(s) 573, 1296, 1452, 1498, 1521
TseFI GTSAC 3 cut(s) 358, 421, 779
Tsp45I GTSAC 3 cut(s) 358, 421, 779
TspGWI ACGGA 1 cut(s) 1407
TspRI CASTG 5 cut(s) 573, 1296, 1452, 1498, 1521
Van91I CCANNNNNTGG 1 cut(s) 231
VpaK11BI GGWCC 1 cut(s) 114
XapI RAATTY 4 cut(s) 350, 865, 1409, 1473
XceI RCATGY 2 cut(s) 1153, 1384
XmiI GTMKAC 2 cut(s) 181, 328
XspI CTAG 1 cut(s) 135
ZrmI AGTACT 1 cut(s) 37
Zsp2I ATGCAT 1 cut(s) 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.