Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0015443

Physical Location & Seq

Genomic Coordinates Reverse (-)

22424662 .. 22426413

Gene Structure 1752 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00100590.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1752 bp

ATGATCAAAACTTTGTCACCCTCCCTCAAACCCTTCTTCATCTTCTTCCCTCATTTTCTCAGACTCATCTTTCATCTTTGCATTCCCACAATGACCACTAATGAAGTCCCACAGCCGAACCCTACTTCGGAACAGATCCTCGACTGGCTTGAGGACTCGGTTTCTTTCTTCCCATCTTTCTTGGACGATCCTTACCAGTCGAATGGTTATCAATGGTGGGATGAAACCCAAGATCTCATTCATGCCAACACCAATTCTCTCGCCAACAACGCTAACATTGCCGTAGCAGAAAATCCAGTCAGCCCTGTTACTCAGTTGCCATCATCGGATTCATCCAAGAAGCGAAAATCCCCGGACGGCCCGGTTTCGGAAACGTCCTCACACATTGACCGGAAGGCGAAAACCGGCCGGCCGGTCAACAAGGCCGAAAACGATGTCAAGAAAGCATCCGATGAAATGGCCACACCGCCAGTTAAGAGAGCAAATGGTAACAAAAAAGGAACAGGGAAAGGTACCGGAAATAACTGTAATAACGGTAATAGCAAGGAAGGGAGGTGGGCAGAGCAGTTGCTCAACCCTTGTGCATCAGCCATCACTGGTGGGAATCTGACACGTGTCCAGCACCTCCTCTACGTCCTCCACGAGCTCGCCTCCCTCACCGGCGACGCCAACCACCGCTTGGCCGCTCACGGCCTCCGAGCGCTGACTCACCACCTGTCATCCAATTCTTCCTCCTCTGCTCCCAATGCTTCCGCCTCAGTAGGACCTGTCGTCACGTTTTCTTCAACAGAACCAAGATTTTTCCAAAAGTCTCTGCTGAAATTCTACGAGGTGAGTCCATGGTTTGCTTTCCCTAACAACATAGCCAACTCTTCAATACTACAAGTACTCGCCGGTGAGCCTAATCGAGCTAGTACTCTTCACATTGTTGACATCGGGGTTTCTCACGGGATGCAATGGCCGACTCTGCTTGAAGCCTTGACTCGCCGTCCAGGTGGGCCTCCTCCATTGGTGAAAATCACAGTCATTGCACCTTCCACAGTCGAGAATGATCAAAAATCTGAAGCACCATTTTCTATTGGTCCTCCTGGTGACAATTTCTCTGCTAGACTTCTCGGTTTCGCAAAATCCATGAACATCAATTTACAGATCAACCGTCTCGACAATCAGCCATTGCAGACACTAAACTCTCAAGTCATTGATGCCTCTAGTGATGAGACATTGATTGTTTGCTTGCAATTTAGGCTGCACCAATTGAACCACAATACTCCTCCGAATGAAAGAACCGAATTCTTGAATGTTCTAAGGAACATGGAGCCGAATGGGGTGATATTAAGCGAGAACAACATGGAATGCAGCTGCAACAGCTGTGGGGATTTCGCAACAGGGTTCTCACGGCAAGTGGAGTACCTTTGGAGGTTTCTAGACTCCACTAGCGCCGCGTTCAAAGGACGTGAGAGCGAGGAGAGGAGGGTGATGGAAGGAGAGGCAGCCAAGGCATTGACCAACCGCGGCGAGATGAATGAAGGGAAGGAGAATTGGTGTGAGAGAATGAAGGGAGTGGGGTTCGTGGGGGAGGTTTTTGGTGAGGATGCCATTGATGGAGGGAGAGCCTTGTTGAGGAAATATGATAGCAATTGGGAAATGAGAGTGGATGAAAAAGATGGGTGTGCAGGATTGTGGTGGAAGGGTCAGCCTGTTTCCTTTTGTTCATTGTGGAAGAATGGATACCAAGTAAGTTATGATCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022603 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0060688 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1900618 GO:1901363 GO:1903506 GO:1905428 GO:2000026 GO:2000032 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

583

Amino Acids

64.42

Weight (kDa)

6.1

Isoelectric Point (pI)

43.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	188 - 574	7.9e-80	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13840
fragaria_vesca	FvH4_2g04140
malus_domestica	MD09G1183000.v1.1
prunus_persica	Prupe.3G040400_v2.0.a1
pyrus_communis	pycom09g10030
rosa_chinensis	RchiOBHm_Chr2g0145631
rosa_laevigata	RLG00000020155
rosa_multiflora	Rmu_sc0003046.1_g000006
rosa_roxburghii	Rroxscaffold_152G00434650 Rroxscaffold_2G00100590
rosa_rugosa	Rorug02G0392400
rosa_samantha	Rh2AG446700 Rh2BG458400 Rh2CG433400 Rh2DG468000
rosa_wichuraiana	Rw2G036480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	512
AccB1I	GGYRCC	1 cut(s)	512
AccB7I	CCANNNNNTGG	1 cut(s)	679
AccBSI	CCGCTC	1 cut(s)	686
AccII	CGCG	2 cut(s)	1442, 1512
AciI	CCGC	7 cut(s)	467, 676, 684, 753, 1440, 1510, 1512
AclWI	GGATC	2 cut(s)	130, 182
AcoI	YGGCCR	5 cut(s)	406, 410, 459, 681, 959
AcsI	RAATTY	2 cut(s)	821, 1289
AcuI	CTGAAG	1 cut(s)	1083
AcvI	CACGTG	1 cut(s)	614
AcyI	GRCGYC	1 cut(s)	666
AfaI	GTAC	4 cut(s)	514, 888, 916, 1409
AfeI	AGCGCT	1 cut(s)	702
AfiI	CCNNNNNNNGG	4 cut(s)	127, 367, 679, 1620
AflIII	ACRYGT	2 cut(s)	611, 613
AgsI	TTSAA	6 cut(s)	786, 876, 974, 1258, 1297, 1447
AhdI	GACNNNNNGTC	2 cut(s)	770, 987
AjiI	CACGTC	1 cut(s)	1454
AjnI	CCWGG	2 cut(s)	991, 1087
AjuI	GAANNNNNNNTTGG	2 cut(s)	222, 254
AluBI	AGCT	4 cut(s)	646, 911, 1359, 1368
AluI	AGCT	4 cut(s)	646, 911, 1359, 1368
Alw21I	GWGCWC	1 cut(s)	648
Alw26I	GTCTC	3 cut(s)	816, 1163, 1211
AlwI	GGATC	2 cut(s)	130, 182
Aor51HI	AGCGCT	1 cut(s)	702
AoxI	GGCC	9 cut(s)	358, 406, 410, 423, 459, 681, 691, 959, 998
ApeKI	GCWGC	4 cut(s)	1246, 1356, 1359, 1490
ApoI	RAATTY	2 cut(s)	821, 1289
ArsI	GACNNNNNNTTYG	2 cut(s)	420, 452
Asp718I	GGTACC	1 cut(s)	512
AspLEI	GCGC	2 cut(s)	703, 1439
AspS9I	GGNCC	4 cut(s)	359, 764, 998, 1082
AsuC2I	CCSGG	2 cut(s)	353, 362
AvaII	GGWCC	2 cut(s)	764, 1082
BalI	TGGCCA	1 cut(s)	461
BanI	GGYRCC	1 cut(s)	512
BanII	GRGCYC	1 cut(s)	648
BarI	GAAGNNNNNNTAC	2 cut(s)	1712, 1744
BauI	CACGAG	1 cut(s)	641
BbrPI	CACGTG	1 cut(s)	614
Bbv12I	GWGCWC	1 cut(s)	648
BbvI	GCAGC	4 cut(s)	1233, 1346, 1368, 1502
BccI	CCATC	6 cut(s)	181, 328, 599, 1471, 1595, 1658
BceAI	ACGGC	5 cut(s)	266, 373, 706, 972, 1412
BciT130I	CCWGG	2 cut(s)	993, 1089
BciVI	GTATCC	1 cut(s)	1721
BclI	TGATCA	3 cut(s)	3, 1051, 1744
BcnI	CCSGG	2 cut(s)	353, 362
BcoDI	GTCTC	3 cut(s)	816, 1163, 1211
BfaI	CTAG	5 cut(s)	912, 1107, 1209, 1424, 1434
BfoI	RGCGCY	2 cut(s)	704, 1440
BfuI	GTATCC	1 cut(s)	1721
BglII	AGATCT	1 cut(s)	232
BisI	GCNGC	7 cut(s)	684, 1247, 1357, 1360, 1440, 1491, 1513
BlsI	GCNGC	7 cut(s)	685, 1248, 1358, 1361, 1441, 1492, 1514
BmcAI	AGTACT	2 cut(s)	888, 916
Bme1390I	CCNGG	4 cut(s)	353, 362, 993, 1089
Bme18I	GGWCC	2 cut(s)	764, 1082
BmeRI	GACNNNNNGTC	2 cut(s)	770, 987
BmgBI	CACGTC	1 cut(s)	1454
BmgT120I	GGNCC	4 cut(s)	359, 764, 998, 1082
BmiI	GGNNCC	2 cut(s)	514, 1317
BmrFI	CCNGG	4 cut(s)	353, 362, 993, 1089
BmsI	GCATC	5 cut(s)	455, 593, 942, 1192, 1582
BoxI	GACNNNNGTC	1 cut(s)	614
BplI	GAGNNNNNCTC	2 cut(s)	635, 667
BpuEI	CTTGAG	2 cut(s)	170, 1176
BpuMI	CCSGG	2 cut(s)	353, 362
BsaAI	YACGTR	1 cut(s)	614
BsaHI	GRCGYC	1 cut(s)	666
BsaJI	CCNNGG	4 cut(s)	351, 839, 1494, 1510
BsaWI	WCCGGW	2 cut(s)	390, 515
BsaXI	ACNNNNNCTCC	2 cut(s)	1526, 1556
Bsc4I	CCNNNNNNNGG	4 cut(s)	127, 367, 679, 1620
Bse118I	RCCGGY	5 cut(s)	404, 408, 412, 659, 893
Bse1I	ACTGG	5 cut(s)	149, 196, 296, 470, 601
Bse3DI	GCAATG	4 cut(s)	276, 962, 1026, 1172
BseBI	CCWGG	2 cut(s)	993, 1089
BseDI	CCNNGG	4 cut(s)	351, 839, 1494, 1510
BseGI	GGATG	7 cut(s)	226, 332, 446, 719, 957, 1597, 1660
BseLI	CCNNNNNNNGG	4 cut(s)	127, 367, 679, 1620
BseMI	GCAATG	4 cut(s)	276, 962, 1026, 1172
BseMII	CTCAG	3 cut(s)	73, 326, 771
BseNI	ACTGG	5 cut(s)	149, 196, 296, 470, 601
BseRI	GAGGAG	6 cut(s)	617, 724, 993, 1260, 1478, 1483
BseX3I	CGGCCG	2 cut(s)	406, 410
BseXI	GCAGC	4 cut(s)	1233, 1346, 1368, 1502
BsgI	GTGCAG	2 cut(s)	1232, 1692
Bsh1236I	CGCG	2 cut(s)	1442, 1512
Bsh1285I	CGRYCG	2 cut(s)	409, 413
BshFI	GGCC	9 cut(s)	360, 408, 412, 425, 461, 683, 693, 961, 1000
BshNI	GGYRCC	1 cut(s)	512
BsiEI	CGRYCG	2 cut(s)	409, 413
BsiHKAI	GWGCWC	1 cut(s)	648
BsiSI	CCGG	9 cut(s)	353, 362, 391, 405, 409, 413, 516, 660, 894
BslFI	GGGAC	1 cut(s)	92
BslI	CCNNNNNNNGG	4 cut(s)	127, 367, 679, 1620
BsmAI	GTCTC	3 cut(s)	816, 1163, 1211
BsmBI	CGTCTC	1 cut(s)	1163
BsmFI	GGGAC	1 cut(s)	92
BsmI	GAATGC	2 cut(s)	81, 1358
BsnI	GGCC	9 cut(s)	360, 408, 412, 425, 461, 683, 693, 961, 1000
Bsp1286I	GDGCHC	1 cut(s)	648
Bsp143I	GATC	7 cut(s)	3, 135, 187, 232, 1051, 1149, 1744
Bsp19I	CCATGG	1 cut(s)	839
BspACI	CCGC	7 cut(s)	467, 676, 684, 753, 1440, 1510, 1512
BspANI	GGCC	9 cut(s)	360, 408, 412, 425, 461, 683, 693, 961, 1000
BspCNI	CTCAG	3 cut(s)	72, 325, 770
BspFNI	CGCG	2 cut(s)	1442, 1512
BspLI	GGNNCC	2 cut(s)	514, 1317
BspPI	GGATC	2 cut(s)	130, 182
BspT107I	GGYRCC	1 cut(s)	512
BsrBI	CCGCTC	1 cut(s)	686
BsrDI	GCAATG	4 cut(s)	276, 962, 1026, 1172
BsrFI	RCCGGY	5 cut(s)	404, 408, 412, 659, 893
BsrI	ACTGG	5 cut(s)	149, 196, 296, 470, 601
BssAI	RCCGGY	5 cut(s)	404, 408, 412, 659, 893
BssECI	CCNNGG	4 cut(s)	351, 839, 1494, 1510
BssMI	GATC	7 cut(s)	3, 135, 187, 232, 1051, 1149, 1744
BssNI	GRCGYC	1 cut(s)	666
BssSI	CACGAG	1 cut(s)	641
BssT1I	CCWWGG	2 cut(s)	839, 1494
Bst2BI	CACGAG	1 cut(s)	641
Bst2UI	CCWGG	2 cut(s)	993, 1089
Bst4CI	ACNGT	5 cut(s)	527, 536, 1024, 1042, 1157
Bst6I	CTCTTC	2 cut(s)	877, 924
BstACI	GRCGYC	1 cut(s)	666
BstBAI	YACGTR	1 cut(s)	614
BstC8I	GCNNGC	3 cut(s)	410, 648, 1235
BstDEI	CTNAG	4 cut(s)	59, 312, 757, 1304
BstDSI	CCRYGG	2 cut(s)	839, 1510
BstENI	CCTNNNNNAGG	1 cut(s)	1618
BstF5I	GGATG	7 cut(s)	226, 332, 446, 719, 957, 1597, 1660
BstFNI	CGCG	2 cut(s)	1442, 1512
BstH2I	RGCGCY	2 cut(s)	704, 1440
BstHHI	GCGC	2 cut(s)	703, 1439
BstKTI	GATC	7 cut(s)	6, 138, 190, 235, 1054, 1152, 1747
BstMAI	GTCTC	3 cut(s)	816, 1163, 1211
BstMBI	GATC	7 cut(s)	3, 135, 187, 232, 1051, 1149, 1744
BstMCI	CGRYCG	2 cut(s)	409, 413
BstMWI	GCNNNNNNNGC	7 cut(s)	269, 278, 746, 967, 1243, 1365, 1496
BstNI	CCWGG	2 cut(s)	993, 1089
BstPAI	GACNNNNGTC	1 cut(s)	614
BstSCI	CCNGG	4 cut(s)	351, 360, 991, 1087
BstUI	CGCG	2 cut(s)	1442, 1512
BstV1I	GCAGC	4 cut(s)	1233, 1346, 1368, 1502
BstX2I	RGATCY	2 cut(s)	135, 232
BstXI	CCANNNNNNTGG	1 cut(s)	203
BstYI	RGATCY	2 cut(s)	135, 232
BstZI	CGGCCG	2 cut(s)	406, 410
BsuI	GTATCC	1 cut(s)	1721
BsuRI	GGCC	9 cut(s)	360, 408, 412, 425, 461, 683, 693, 961, 1000
BtgI	CCRYGG	2 cut(s)	839, 1510
BtrI	CACGTC	1 cut(s)	1454
BtsCI	GGATG	7 cut(s)	226, 332, 446, 719, 957, 1597, 1660
BtsIMutI	CAGTG	1 cut(s)	594
Cac8I	GCNNGC	3 cut(s)	410, 648, 1235
CfoI	GCGC	2 cut(s)	703, 1439
Cfr10I	RCCGGY	5 cut(s)	404, 408, 412, 659, 893
Cfr13I	GGNCC	4 cut(s)	359, 764, 998, 1082
Cfr42I	CCGCGG	1 cut(s)	1513
CseI	GACGC	1 cut(s)	674
Csp6I	GTAC	4 cut(s)	513, 887, 915, 1408
CspCI	CAANNNNNGTGG	2 cut(s)	1351, 1386
CviAII	CATG	5 cut(s)	242, 840, 1132, 1312, 1348
CviQI	GTAC	4 cut(s)	513, 887, 915, 1408
DdeI	CTNAG	4 cut(s)	59, 312, 757, 1304
DpnI	GATC	7 cut(s)	5, 137, 189, 234, 1053, 1151, 1746
DpnII	GATC	7 cut(s)	3, 135, 187, 232, 1051, 1149, 1744
DriI	GACNNNNNGTC	2 cut(s)	770, 987
EaeI	YGGCCR	5 cut(s)	406, 410, 459, 681, 959
EagI	CGGCCG	2 cut(s)	406, 410
Eam1104I	CTCTTC	2 cut(s)	877, 924
Eam1105I	GACNNNNNGTC	2 cut(s)	770, 987
EarI	CTCTTC	2 cut(s)	877, 924
EciI	GGCGGA	1 cut(s)	742
Ecl136II	GAGCTC	1 cut(s)	646
EclXI	CGGCCG	2 cut(s)	406, 410
Eco130I	CCWWGG	2 cut(s)	839, 1494
Eco24I	GRGCYC	1 cut(s)	648
Eco47I	GGWCC	2 cut(s)	764, 1082
Eco47III	AGCGCT	1 cut(s)	702
Eco52I	CGGCCG	2 cut(s)	406, 410
Eco53kI	GAGCTC	1 cut(s)	646
Eco57I	CTGAAG	1 cut(s)	1083
Eco72I	CACGTG	1 cut(s)	614
EcoICRI	GAGCTC	1 cut(s)	646
EcoNI	CCTNNNNNAGG	1 cut(s)	1618
EcoO109I	RGGNCCY	1 cut(s)	764
EcoRI	GAATTC	1 cut(s)	1289
EcoRII	CCWGG	2 cut(s)	991, 1087
EcoT14I	CCWWGG	2 cut(s)	839, 1494
EcoT38I	GRGCYC	1 cut(s)	648
ErhI	CCWWGG	2 cut(s)	839, 1494
Esp3I	CGTCTC	1 cut(s)	1163
FaeI	CATG	5 cut(s)	245, 843, 1135, 1315, 1351
FaiI	YATR	8 cut(s)	243, 841, 863, 1133, 1313, 1349, 1629, 1743
FaqI	GGGAC	1 cut(s)	92
FatI	CATG	5 cut(s)	241, 839, 1131, 1311, 1347
FbaI	TGATCA	3 cut(s)	3, 1051, 1744
Fnu4HI	GCNGC	7 cut(s)	684, 1247, 1357, 1360, 1440, 1491, 1513
FokI	GGATG	7 cut(s)	233, 319, 433, 706, 964, 1604, 1667
FriOI	GRGCYC	1 cut(s)	648
FseI	GGCCGGCC	1 cut(s)	412
Fsp4HI	GCNGC	7 cut(s)	684, 1247, 1357, 1360, 1440, 1491, 1513
FspBI	CTAG	5 cut(s)	912, 1107, 1209, 1424, 1434
GlaI	GCGC	2 cut(s)	702, 1438
GluI	GCNGC	7 cut(s)	684, 1247, 1357, 1360, 1440, 1491, 1513
HaeII	RGCGCY	2 cut(s)	704, 1440
HaeIII	GGCC	9 cut(s)	360, 408, 412, 425, 461, 683, 693, 961, 1000
HapII	CCGG	9 cut(s)	353, 362, 391, 405, 409, 413, 516, 660, 894
HgaI	GACGC	1 cut(s)	674
HhaI	GCGC	2 cut(s)	703, 1439
Hin1I	GRCGYC	1 cut(s)	666
Hin1II	CATG	5 cut(s)	245, 843, 1135, 1315, 1351
Hin6I	GCGC	2 cut(s)	701, 1437
HinP1I	GCGC	2 cut(s)	701, 1437
HincII	GTYRAC	2 cut(s)	418, 931
HindII	GTYRAC	2 cut(s)	418, 931
HinfI	GANTC	9 cut(s)	63, 155, 329, 604, 706, 835, 964, 982, 1427
HpaII	CCGG	9 cut(s)	353, 362, 391, 405, 409, 413, 516, 660, 894
Hpy166II	GTNNAC	2 cut(s)	418, 931
Hpy188I	TCNGA	9 cut(s)	62, 130, 328, 370, 451, 609, 698, 1063, 1275
Hpy188III	TCNNGA	5 cut(s)	439, 1045, 1160, 1294, 1424
Hpy8I	GTNNAC	2 cut(s)	418, 931
Hpy99I	CGWCG	1 cut(s)	668
HpyAV	CCTTC	9 cut(s)	43, 388, 542, 1044, 1475, 1520, 1525, 1549, 1681
HpyCH4III	ACNGT	5 cut(s)	527, 536, 1024, 1042, 1157
HpyCH4IV	ACGT	5 cut(s)	374, 613, 633, 776, 1453
HpyF10VI	GCNNNNNNNGC	7 cut(s)	269, 278, 746, 967, 1243, 1365, 1496
HpyF3I	CTNAG	4 cut(s)	59, 312, 757, 1304
HpySE526I	ACGT	5 cut(s)	374, 613, 633, 776, 1453
Hsp92I	GRCGYC	1 cut(s)	666
Hsp92II	CATG	5 cut(s)	245, 843, 1135, 1315, 1351
HspAI	GCGC	2 cut(s)	701, 1437
KpnI	GGTACC	1 cut(s)	516
KroI	GCCGGC	1 cut(s)	408
KroNI	GCCGGC	1 cut(s)	410
Ksp22I	TGATCA	3 cut(s)	3, 1051, 1744
KspI	CCGCGG	1 cut(s)	1513
Kzo9I	GATC	7 cut(s)	3, 135, 187, 232, 1051, 1149, 1744
LmnI	GCTCC	2 cut(s)	745, 1315
Lsp1109I	GCAGC	4 cut(s)	1233, 1346, 1368, 1502
LweI	GCATC	5 cut(s)	455, 593, 942, 1192, 1582
MaeI	CTAG	5 cut(s)	912, 1107, 1209, 1424, 1434
MaeII	ACGT	5 cut(s)	374, 613, 633, 776, 1453
MaeIII	GTNAC	5 cut(s)	15, 307, 488, 772, 1091
MalI	GATC	7 cut(s)	5, 137, 189, 234, 1053, 1151, 1746
MbiI	CCGCTC	1 cut(s)	686
MboI	GATC	7 cut(s)	3, 135, 187, 232, 1051, 1149, 1744
MboII	GAAGA	9 cut(s)	28, 34, 37, 160, 720, 774, 864, 911, 1732
MfeI	CAATTG	2 cut(s)	1253, 1636
MflI	RGATCY	2 cut(s)	135, 232
MhlI	GDGCHC	1 cut(s)	648
MlsI	TGGCCA	1 cut(s)	461
MluNI	TGGCCA	1 cut(s)	461
MlyI	GAGTC	7 cut(s)	57, 149, 700, 844, 958, 976, 1421
Mox20I	TGGCCA	1 cut(s)	461
MroNI	GCCGGC	1 cut(s)	408
MscI	TGGCCA	1 cut(s)	461
MseI	TTAA	2 cut(s)	474, 1334
Msp20I	TGGCCA	1 cut(s)	461
MspA1I	CMGCKG	3 cut(s)	1359, 1368, 1512
MspI	CCGG	9 cut(s)	353, 362, 391, 405, 409, 413, 516, 660, 894
MspR9I	CCNGG	4 cut(s)	353, 362, 993, 1089
MunI	CAATTG	2 cut(s)	1253, 1636
Mva1269I	GAATGC	2 cut(s)	81, 1358
MvaI	CCWGG	2 cut(s)	993, 1089
MvnI	CGCG	2 cut(s)	1442, 1512
MwoI	GCNNNNNNNGC	7 cut(s)	269, 278, 746, 967, 1243, 1365, 1496
NaeI	GCCGGC	1 cut(s)	410
NciI	CCSGG	2 cut(s)	353, 362
NcoI	CCATGG	1 cut(s)	839
NdeII	GATC	7 cut(s)	3, 135, 187, 232, 1051, 1149, 1744
NgoMIV	GCCGGC	1 cut(s)	408
NlaIII	CATG	5 cut(s)	245, 843, 1135, 1315, 1351
NlaIV	GGNNCC	2 cut(s)	514, 1317
NmuCI	GTSAC	3 cut(s)	15, 772, 1091
PcsI	WCGNNNNNNNCGW	1 cut(s)	639
PctI	GAATGC	2 cut(s)	81, 1358
PdiI	GCCGGC	1 cut(s)	410
PfeI	GAWTC	2 cut(s)	329, 604
PflMI	CCANNNNNTGG	1 cut(s)	679
PkrI	GCNGC	7 cut(s)	685, 1248, 1358, 1361, 1441, 1492, 1514
PleI	GAGTC	7 cut(s)	57, 149, 700, 843, 958, 976, 1421
PmaCI	CACGTG	1 cut(s)	614
PmlI	CACGTG	1 cut(s)	614
PpsI	GAGTC	7 cut(s)	57, 149, 700, 843, 958, 976, 1421
Ppu21I	YACGTR	1 cut(s)	614
PpuMI	RGGWCCY	1 cut(s)	764
PshAI	GACNNNNGTC	1 cut(s)	614
Psp124BI	GAGCTC	1 cut(s)	648
Psp5II	RGGWCCY	1 cut(s)	764
Psp6I	CCWGG	2 cut(s)	991, 1087
PspCI	CACGTG	1 cut(s)	614
PspGI	CCWGG	2 cut(s)	991, 1087
PspN4I	GGNNCC	2 cut(s)	514, 1317
PspPI	GGNCC	4 cut(s)	359, 764, 998, 1082
PspPPI	RGGWCCY	1 cut(s)	764
PsuI	RGATCY	2 cut(s)	135, 232
PvuII	CAGCTG	2 cut(s)	1359, 1368
RigI	GGCCGGCC	1 cut(s)	412
RsaI	GTAC	4 cut(s)	514, 888, 916, 1409
RsaNI	GTAC	4 cut(s)	513, 887, 915, 1408
SacI	GAGCTC	1 cut(s)	648
SacII	CCGCGG	1 cut(s)	1513
SaqAI	TTAA	2 cut(s)	474, 1334
SatI	GCNGC	7 cut(s)	684, 1247, 1357, 1360, 1440, 1491, 1513
Sau3AI	GATC	7 cut(s)	3, 135, 187, 232, 1051, 1149, 1744
Sau96I	GGNCC	4 cut(s)	359, 764, 998, 1082
ScaI	AGTACT	2 cut(s)	888, 916
SchI	GAGTC	7 cut(s)	57, 149, 700, 844, 958, 976, 1421
ScrFI	CCNGG	4 cut(s)	353, 362, 993, 1089
SduI	GDGCHC	1 cut(s)	648
SfaNI	GCATC	5 cut(s)	455, 593, 942, 1192, 1582
Sfr303I	CCGCGG	1 cut(s)	1513
SgrAI	CRCCGGYG	2 cut(s)	659, 893
SgrBI	CCGCGG	1 cut(s)	1513
SinI	GGWCC	2 cut(s)	764, 1082
SmlI	CTYRAG	2 cut(s)	149, 1191
SmoI	CTYRAG	2 cut(s)	149, 1191
SsiI	CCGC	7 cut(s)	467, 676, 684, 753, 1440, 1510, 1512
SspMI	CTAG	5 cut(s)	912, 1107, 1209, 1424, 1434
SstI	GAGCTC	1 cut(s)	648
StyD4I	CCNGG	4 cut(s)	351, 360, 991, 1087
StyI	CCWWGG	2 cut(s)	839, 1494
TaaI	ACNGT	5 cut(s)	527, 536, 1024, 1042, 1157
TaiI	ACGT	5 cut(s)	377, 616, 636, 779, 1456
TaqI	TCGA	5 cut(s)	141, 200, 907, 1044, 1161
TatI	WGTACW	2 cut(s)	886, 914
TauI	GCSGC	3 cut(s)	686, 1442, 1515
TfiI	GAWTC	2 cut(s)	329, 604
Tru1I	TTAA	2 cut(s)	474, 1334
Tru9I	TTAA	2 cut(s)	474, 1334
TscAI	CASTG	1 cut(s)	601
TseFI	GTSAC	3 cut(s)	15, 772, 1091
TseI	GCWGC	4 cut(s)	1246, 1356, 1359, 1490
Tsp45I	GTSAC	3 cut(s)	15, 772, 1091
TspRI	CASTG	1 cut(s)	601
Van91I	CCANNNNNTGG	1 cut(s)	679
VpaK11BI	GGWCC	2 cut(s)	764, 1082
XagI	CCTNNNNNAGG	1 cut(s)	1618
XapI	RAATTY	2 cut(s)	821, 1289
XbaI	TCTAGA	1 cut(s)	1423
XcmI	CCANNNNNNNNNTGG	1 cut(s)	676
XspI	CTAG	5 cut(s)	912, 1107, 1209, 1424, 1434
ZrmI	AGTACT	2 cut(s)	888, 916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.