Rroxscaffold_2G00127650

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019867
Physical Location & Seq
Reverse (-)
63255598 .. 63256709
1112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127650.1

Sequence Viewer

Length: 801 bp
ATGCCCTCGAGTCTCAGTTACAGCCTACCGCCGCCGGAGGCAGCAGGTCGGCGTTCTCCGAGCAAGCAGAGGCAGATCAAATATCTTTACAGCCACGTGTCACAGGGCGAGGGCGGCGACGGCGACGGGATGATGTCAAAAGAGTCGATTCAGATCAACTTTGTGGTCCAAACGTCGAGAGGAAGCTGCTGGGTTGGGGACCCAAACGCCGTCGTTTACGGCCAATTTCACCTCAAGGGTCGCTCAACCATCAACGACGACGACTTGTCTTCAAGACCCAGCCTTTCAAGCAAACTGGGTCGGAAGCTTTCGGGGTGGGGTGTGCCTCAACCTCAGCAGGCAACGATTCTTGAACAGGTGTTTCGGGTGCTCGAAAAGGCCAAGGCTGCCGTCCCCGTAGCTGTCACCATCCAGAAATCGACTGTGTTCATTTTCGATAGTTTGAAAGACCACCAAACCATGGTGGAGCATATCAAGAAGACCGATTCGGAGCATTTTGAGGGTTTGGGGAAAGTGACGTCAGTTGGTGACGTCATCCCTCCTCCATCATGCTCTATTTGTTTGGAGGGCGTTTCACAAGCCCAAGCTCAAAGTGGCGGTGTTGCTCGCTTGCCCTGTTCCCACATTTATCACAAAAACTGCATTCTCCCGTGGCTCAAGAAGAGTCATTTGTGTCCCATTTGCCGACACCCTGACCCAATTTCCCAAGCCCAATTGGGCAAACCCTCAAAACCCCGCCTCCTCCGCGTAGGGCGGCGGGGGTGGTGGTCTCGCTTGTGGAAGAGCTTTAGGACTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

266

Amino Acids

29.32

Weight (kDa)

9.71

Isoelectric Point (pI)

55.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 183 - 229 9e-13 Ring finger domain
zf-RING_11 PF17123 184 - 215 1.1e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 184 - 228 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 184 - 228 8.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_Vps41 PF23555 184 - 228 1.9e-06 Vps41 C-terminal RING finger domain
zf-RING_UBOX PF13445 184 - 226 2.9e-06 RING-type zinc-finger
zf-rbx1 PF12678 199 - 229 4.9e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019867)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0115561
rosa_roxburghii Rroxscaffold_2G00127650 Rroxscaffold_2G00127730 Rroxscaffold_2G00128480 Rroxscaffold_2G00128570
rosa_samantha Rh2BG266200 Rh2CG260600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 521, 534
Acc36I ACCTGC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 460
AccII CGCG 1 cut(s) 747
AciI CCGC 8 cut(s) 29, 32, 114, 597, 736, 745, 754, 757
AcoI YGGCCR 1 cut(s) 220
AcvI CACGTG 1 cut(s) 97
AcyI GRCGYC 2 cut(s) 518, 531
AfiI CCNNNNNNNGG 1 cut(s) 460
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 4 cut(s) 273, 288, 353, 445
AhdI GACNNNNNGTC 1 cut(s) 265
AhlI ACTAGT 1 cut(s) 794
AluBI AGCT 5 cut(s) 186, 307, 401, 587, 786
AluI AGCT 5 cut(s) 186, 307, 401, 587, 786
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 2 cut(s) 17, 774
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 2 cut(s) 220, 378
ApeKI GCWGC 3 cut(s) 41, 186, 386
AspS9I GGNCC 2 cut(s) 166, 199
AsuHPI GGTGA 3 cut(s) 221, 397, 539
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 2 cut(s) 166, 199
BbrPI CACGTG 1 cut(s) 97
BbsI GAAGAC 2 cut(s) 261, 485
Bbv12I GWGCWC 1 cut(s) 372
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 3 cut(s) 53, 173, 373
BccI CCATC 3 cut(s) 257, 416, 553
BceAI ACGGC 4 cut(s) 136, 194, 235, 374
BcoDI GTCTC 2 cut(s) 17, 774
BcuI ACTAGT 1 cut(s) 794
BfaI CTAG 1 cut(s) 795
BfuAI ACCTGC 1 cut(s) 35
BisI GCNGC 6 cut(s) 32, 42, 115, 187, 387, 755
BlsI GCNGC 6 cut(s) 33, 43, 116, 188, 388, 756
Bme18I GGWCC 2 cut(s) 166, 199
BmeRI GACNNNNNGTC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 166, 199
BmiI GGNNCC 2 cut(s) 200, 201
BmrI ACTGGG 1 cut(s) 305
BmuI ACTGGG 1 cut(s) 305
BpiI GAAGAC 2 cut(s) 261, 485
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 2 cut(s) 218, 641
BsaAI YACGTR 1 cut(s) 97
BsaBI GATNNNNATC 1 cut(s) 152
BsaHI GRCGYC 2 cut(s) 518, 531
BsaI GGTCTC 1 cut(s) 774
BsaJI CCNNGG 3 cut(s) 381, 459, 650
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 300
Bse8I GATNNNNATC 1 cut(s) 152
BseDI CCNNGG 3 cut(s) 381, 459, 650
BseGI GGATG 3 cut(s) 135, 408, 534
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 1 cut(s) 460
BseMII CTCAG 2 cut(s) 28, 347
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 2 cut(s) 531, 731
BseXI GCAGC 3 cut(s) 53, 173, 373
BseYI CCCAGC 2 cut(s) 189, 278
Bsh1236I CGCG 1 cut(s) 747
BshFI GGCC 2 cut(s) 222, 380
BsiHKAI GWGCWC 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 212, 377, 660
BslI CCNNNNNNNGG 1 cut(s) 460
BsmAI GTCTC 2 cut(s) 17, 774
BsmFI GGGAC 3 cut(s) 212, 377, 660
BsmI GAATGC 1 cut(s) 642
BsnI GGCC 2 cut(s) 222, 380
Bso31I GGTCTC 1 cut(s) 774
BsoBI CYCGRG 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 372
Bsp143I GATC 2 cut(s) 75, 153
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 8 cut(s) 29, 32, 114, 597, 736, 745, 754, 757
BspANI GGCC 2 cut(s) 222, 380
BspCNI CTCAG 2 cut(s) 27, 346
BspFNI CGCG 1 cut(s) 747
BspLI GGNNCC 2 cut(s) 200, 201
BspMI ACCTGC 1 cut(s) 35
BspQI GCTCTTC 1 cut(s) 776
BspTNI GGTCTC 1 cut(s) 774
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 3 cut(s) 381, 459, 650
BssMI GATC 2 cut(s) 75, 153
BssNI GRCGYC 2 cut(s) 518, 531
BssT1I CCWWGG 2 cut(s) 381, 459
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 2 cut(s) 656, 776
BstACI GRCGYC 2 cut(s) 518, 531
BstBAI YACGTR 1 cut(s) 97
BstC8I GCNNGC 4 cut(s) 65, 339, 607, 611
BstDEI CTNAG 2 cut(s) 14, 333
BstDSI CCRYGG 2 cut(s) 459, 650
BstF5I GGATG 3 cut(s) 135, 408, 534
BstFNI CGCG 1 cut(s) 747
BstKTI GATC 2 cut(s) 78, 156
BstMAI GTCTC 2 cut(s) 17, 774
BstMBI GATC 2 cut(s) 75, 153
BstMWI GCNNNNNNNGC 5 cut(s) 114, 120, 288, 386, 744
BstUI CGCG 1 cut(s) 747
BstV1I GCAGC 3 cut(s) 53, 173, 373
BstV2I GAAGAC 2 cut(s) 261, 485
BsuRI GGCC 2 cut(s) 222, 380
BtgI CCRYGG 2 cut(s) 459, 650
BtsCI GGATG 3 cut(s) 135, 408, 534
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 4 cut(s) 65, 339, 607, 611
Cfr13I GGNCC 2 cut(s) 166, 199
CviAII CATG 2 cut(s) 460, 549
DdeI CTNAG 2 cut(s) 14, 333
DpnI GATC 2 cut(s) 77, 155
DpnII GATC 2 cut(s) 75, 153
DriI GACNNNNNGTC 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 2 cut(s) 656, 776
Eam1105I GACNNNNNGTC 1 cut(s) 265
EarI CTCTTC 2 cut(s) 656, 776
Eco130I CCWWGG 2 cut(s) 381, 459
Eco31I GGTCTC 1 cut(s) 774
Eco47I GGWCC 2 cut(s) 166, 199
Eco72I CACGTG 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 199
EcoT14I CCWWGG 2 cut(s) 381, 459
ErhI CCWWGG 2 cut(s) 381, 459
FaeI CATG 2 cut(s) 463, 552
FaiI YATR 3 cut(s) 461, 471, 550
FaqI GGGAC 3 cut(s) 212, 377, 660
FatI CATG 2 cut(s) 459, 548
FauI CCCGC 2 cut(s) 743, 750
Fnu4HI GCNGC 6 cut(s) 32, 42, 115, 187, 387, 755
FokI GGATG 3 cut(s) 142, 395, 521
Fsp4HI GCNGC 6 cut(s) 32, 42, 115, 187, 387, 755
FspBI CTAG 1 cut(s) 795
GluI GCNGC 6 cut(s) 32, 42, 115, 187, 387, 755
GsaI CCCAGC 2 cut(s) 193, 282
HaeIII GGCC 2 cut(s) 222, 380
HapII CCGG 1 cut(s) 35
Hin1I GRCGYC 2 cut(s) 518, 531
Hin1II CATG 2 cut(s) 463, 552
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 6 cut(s) 10, 143, 148, 346, 485, 664
HpaII CCGG 1 cut(s) 35
HphI GGTGA 3 cut(s) 221, 397, 539
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 4 cut(s) 60, 153, 303, 490
Hpy188III TCNNGA 6 cut(s) 177, 273, 350, 412, 475, 658
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 6 cut(s) 122, 128, 178, 215, 260, 263
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 4 cut(s) 96, 173, 518, 531
HpyCH4V TGCA 1 cut(s) 642
HpyF10VI GCNNNNNNNGC 5 cut(s) 114, 120, 288, 386, 744
HpyF3I CTNAG 2 cut(s) 14, 333
HpySE526I ACGT 4 cut(s) 96, 173, 518, 531
Hsp92I GRCGYC 2 cut(s) 518, 531
Hsp92II CATG 2 cut(s) 463, 552
KflI GGGWCCC 1 cut(s) 199
Kzo9I GATC 2 cut(s) 75, 153
LguI GCTCTTC 1 cut(s) 776
LmnI GCTCC 2 cut(s) 466, 490
Lsp1109I GCAGC 3 cut(s) 53, 173, 373
MaeI CTAG 1 cut(s) 795
MaeII ACGT 4 cut(s) 96, 173, 518, 531
MaeIII GTNAC 5 cut(s) 17, 99, 403, 514, 527
MalI GATC 2 cut(s) 77, 155
MboI GATC 2 cut(s) 75, 153
MboII GAAGA 4 cut(s) 261, 490, 673, 793
MfeI CAATTG 1 cut(s) 713
MhlI GDGCHC 1 cut(s) 372
MluCI AATT 3 cut(s) 224, 699, 713
MlyI GAGTC 3 cut(s) 19, 152, 673
MmeI TCCRAC 1 cut(s) 281
MspI CCGG 1 cut(s) 35
MunI CAATTG 1 cut(s) 713
Mva1269I GAATGC 1 cut(s) 642
MvnI CGCG 1 cut(s) 747
MwoI GCNNNNNNNGC 5 cut(s) 114, 120, 288, 386, 744
NcoI CCATGG 1 cut(s) 459
NdeII GATC 2 cut(s) 75, 153
NlaIII CATG 2 cut(s) 463, 552
NlaIV GGNNCC 2 cut(s) 200, 201
NmuCI GTSAC 4 cut(s) 99, 403, 514, 527
PaeR7I CTCGAG 1 cut(s) 7
PciSI GCTCTTC 1 cut(s) 776
PctI GAATGC 1 cut(s) 642
PfeI GAWTC 3 cut(s) 148, 346, 485
PflMI CCANNNNNTGG 1 cut(s) 460
PkrI GCNGC 6 cut(s) 33, 43, 116, 188, 388, 756
PleI GAGTC 3 cut(s) 18, 151, 672
PmaCI CACGTG 1 cut(s) 97
PmlI CACGTG 1 cut(s) 97
PpsI GAGTC 3 cut(s) 18, 151, 672
Ppu21I YACGTR 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 199
Psp5II RGGWCCY 1 cut(s) 199
PspCI CACGTG 1 cut(s) 97
PspFI CCCAGC 2 cut(s) 189, 278
PspN4I GGNNCC 2 cut(s) 200, 201
PspPI GGNCC 2 cut(s) 166, 199
PspPPI RGGWCCY 1 cut(s) 199
PspXI VCTCGAGB 1 cut(s) 7
SapI GCTCTTC 1 cut(s) 776
SatI GCNGC 6 cut(s) 32, 42, 115, 187, 387, 755
Sau3AI GATC 2 cut(s) 75, 153
Sau96I GGNCC 2 cut(s) 166, 199
SchI GAGTC 3 cut(s) 19, 152, 673
SduI GDGCHC 1 cut(s) 372
Sfr274I CTCGAG 1 cut(s) 7
SinI GGWCC 2 cut(s) 166, 199
SlaI CTCGAG 1 cut(s) 7
SmlI CTYRAG 3 cut(s) 7, 233, 656
SmoI CTYRAG 3 cut(s) 7, 233, 656
SpeI ACTAGT 1 cut(s) 794
Sse9I AATT 3 cut(s) 224, 699, 713
SsiI CCGC 8 cut(s) 29, 32, 114, 597, 736, 745, 754, 757
SspMI CTAG 1 cut(s) 795
StyI CCWWGG 2 cut(s) 381, 459
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 4 cut(s) 99, 176, 521, 534
TaqI TCGA 6 cut(s) 8, 146, 176, 372, 419, 435
TaqII GACCGA 1 cut(s) 497
TasI AATT 3 cut(s) 224, 699, 713
TauI GCSGC 3 cut(s) 34, 117, 757
TfiI GAWTC 3 cut(s) 148, 346, 485
TseFI GTSAC 4 cut(s) 99, 403, 514, 527
TseI GCWGC 3 cut(s) 41, 186, 386
Tsp45I GTSAC 4 cut(s) 99, 403, 514, 527
TspDTI ATGAA 1 cut(s) 418
Van91I CCANNNNNTGG 1 cut(s) 460
VpaK11BI GGWCC 2 cut(s) 166, 199
XcmI CCANNNNNNNNNTGG 1 cut(s) 590
XhoI CTCGAG 1 cut(s) 7
XspI CTAG 1 cut(s) 795
ZraI GACGTC 2 cut(s) 519, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.