Rroxscaffold_2G00127730

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019867
Physical Location & Seq
Forward (+)
63329478 .. 63330284
807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127730.1

Sequence Viewer

Length: 807 bp
ATGCCCTCGAGTCTCAGTTACAGCCTACCGCCGCCGGAGGCAGCAGGTCGGCGTTCTCCGAGCAAGCAGAGGCAGATCAAATATCTTTACAGCCACGTGTCACAGGGCGAGGGCGGCGACGGTTACGGCGACGGGATGATGTCAAAAGAGTCGATTCAGATCAACTTTGTGGTCCAAACGTCGAGAGGAAGCTGCTGGGTTGGGGACCCAAACGCCGTCGTTTACGGCCAATTTCACCTCAAAGGTCGCTCAACCATCAACGACGACGACTTGTCTTCAAGACCCAGCCTTTCAAGCAAACTGGGTCGGAAGCTTTCGGGGTGGGGTGTGCCTCAACCTCAGCAGGCAACGATTCTTGAACAGGTGTTTCGGGTGCTCGAAAAGGCCAAGGCTGCCGTCCCCGTAGCTGTCACCATCCAGAAATCGACTGTGTTCATTTTCGATAGTTTGAAAGACCACCAAACCATGGTGGAGCATATCAAGAAGACCGATTCGGAGCATTTTGAGGGTTTGGGGAAAGTGACGTCAGTTGGTGACGTCATCCCTCCTCCATCATGCTCTATTTGTTTGGAGGGCGTTTCACAAGCCCAAGCTCAAAGTGGCGGTGTTGCTCGCTTGCCCTGTTCCCACATTTATCACAAAAACTGCATTCTCCCGTGGCTCAAGAAGAGTCATTTGTGTCCCATTTGCCGACACCCTGACCCAATTTCCCAAGCCCAATTGGGCAAACCCTCAAAACCCTGCCTCCTCCGCGTAGGGCGGCGGGGGTGGTGGTCTCGCTTGTGGAAGAGGTTTAGGGCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

268

Amino Acids

29.53

Weight (kDa)

9.63

Isoelectric Point (pI)

56.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 185 - 231 9.1e-13 Ring finger domain
zf-RING_11 PF17123 186 - 217 1.1e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 186 - 230 8.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 186 - 230 9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_Vps41 PF23555 186 - 230 2.1e-06 Vps41 C-terminal RING finger domain
zf-RING_UBOX PF13445 186 - 228 2.9e-06 RING-type zinc-finger
zf-rbx1 PF12678 201 - 231 5.3e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019867)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0115561
rosa_roxburghii Rroxscaffold_2G00127650 Rroxscaffold_2G00127730 Rroxscaffold_2G00128480 Rroxscaffold_2G00128570
rosa_samantha Rh2BG266200 Rh2CG260600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 527, 540
Acc36I ACCTGC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 466
AccII CGCG 1 cut(s) 753
AciI CCGC 7 cut(s) 29, 32, 114, 603, 751, 760, 763
AcoI YGGCCR 1 cut(s) 226
AcvI CACGTG 1 cut(s) 97
AcyI GRCGYC 2 cut(s) 524, 537
AfiI CCNNNNNNNGG 1 cut(s) 466
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 4 cut(s) 279, 294, 359, 451
AhdI GACNNNNNGTC 1 cut(s) 271
AluBI AGCT 4 cut(s) 192, 313, 407, 593
AluI AGCT 4 cut(s) 192, 313, 407, 593
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 2 cut(s) 17, 780
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 2 cut(s) 226, 384
ApeKI GCWGC 3 cut(s) 41, 192, 392
AspS9I GGNCC 2 cut(s) 172, 205
AsuHPI GGTGA 3 cut(s) 227, 403, 545
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 2 cut(s) 172, 205
BbrPI CACGTG 1 cut(s) 97
BbsI GAAGAC 2 cut(s) 267, 491
Bbv12I GWGCWC 1 cut(s) 378
BbvCI CCTCAGC 1 cut(s) 339
BbvI GCAGC 3 cut(s) 53, 179, 379
BccI CCATC 3 cut(s) 263, 422, 559
BceAI ACGGC 4 cut(s) 142, 200, 241, 380
BcoDI GTCTC 2 cut(s) 17, 780
BfaI CTAG 1 cut(s) 801
BfuAI ACCTGC 1 cut(s) 35
BisI GCNGC 6 cut(s) 32, 42, 115, 193, 393, 761
BlsI GCNGC 6 cut(s) 33, 43, 116, 194, 394, 762
Bme18I GGWCC 2 cut(s) 172, 205
BmeRI GACNNNNNGTC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 172, 205
BmiI GGNNCC 2 cut(s) 206, 207
BmrI ACTGGG 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 311
BpiI GAAGAC 2 cut(s) 267, 491
Bpu10I CCTNAGC 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 647
BsaAI YACGTR 1 cut(s) 97
BsaBI GATNNNNATC 1 cut(s) 158
BsaHI GRCGYC 2 cut(s) 524, 537
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 3 cut(s) 387, 465, 656
BsaXI ACNNNNNCTCC 2 cut(s) 729, 759
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse1I ACTGG 1 cut(s) 306
Bse8I GATNNNNATC 1 cut(s) 158
BseDI CCNNGG 3 cut(s) 387, 465, 656
BseGI GGATG 3 cut(s) 141, 414, 540
BseJI GATNNNNATC 1 cut(s) 158
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMII CTCAG 2 cut(s) 28, 353
BseNI ACTGG 1 cut(s) 306
BseRI GAGGAG 2 cut(s) 537, 737
BseXI GCAGC 3 cut(s) 53, 179, 379
BseYI CCCAGC 2 cut(s) 195, 284
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 2 cut(s) 228, 386
BsiHKAI GWGCWC 1 cut(s) 378
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 218, 383, 666
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 2 cut(s) 17, 780
BsmFI GGGAC 3 cut(s) 218, 383, 666
BsmI GAATGC 1 cut(s) 648
BsnI GGCC 2 cut(s) 228, 386
Bso31I GGTCTC 1 cut(s) 780
BsoBI CYCGRG 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 2 cut(s) 75, 159
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 7 cut(s) 29, 32, 114, 603, 751, 760, 763
BspANI GGCC 2 cut(s) 228, 386
BspCNI CTCAG 2 cut(s) 27, 352
BspFNI CGCG 1 cut(s) 753
BspLI GGNNCC 2 cut(s) 206, 207
BspMI ACCTGC 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 780
BsrI ACTGG 1 cut(s) 306
BssECI CCNNGG 3 cut(s) 387, 465, 656
BssMI GATC 2 cut(s) 75, 159
BssNI GRCGYC 2 cut(s) 524, 537
BssT1I CCWWGG 2 cut(s) 387, 465
Bst4CI ACNGT 2 cut(s) 122, 430
Bst6I CTCTTC 2 cut(s) 662, 782
BstACI GRCGYC 2 cut(s) 524, 537
BstBAI YACGTR 1 cut(s) 97
BstC8I GCNNGC 4 cut(s) 65, 345, 613, 617
BstDEI CTNAG 2 cut(s) 14, 339
BstDSI CCRYGG 2 cut(s) 465, 656
BstF5I GGATG 3 cut(s) 141, 414, 540
BstFNI CGCG 1 cut(s) 753
BstKTI GATC 2 cut(s) 78, 162
BstMAI GTCTC 2 cut(s) 17, 780
BstMBI GATC 2 cut(s) 75, 159
BstMWI GCNNNNNNNGC 4 cut(s) 114, 294, 392, 750
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 3 cut(s) 53, 179, 379
BstV2I GAAGAC 2 cut(s) 267, 491
BsuRI GGCC 2 cut(s) 228, 386
BtgI CCRYGG 2 cut(s) 465, 656
BtsCI GGATG 3 cut(s) 141, 414, 540
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 4 cut(s) 65, 345, 613, 617
Cfr13I GGNCC 2 cut(s) 172, 205
CviAII CATG 2 cut(s) 466, 555
DdeI CTNAG 2 cut(s) 14, 339
DpnI GATC 2 cut(s) 77, 161
DpnII GATC 2 cut(s) 75, 159
DriI GACNNNNNGTC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 662, 782
Eam1105I GACNNNNNGTC 1 cut(s) 271
EarI CTCTTC 2 cut(s) 662, 782
Eco130I CCWWGG 2 cut(s) 387, 465
Eco31I GGTCTC 1 cut(s) 780
Eco47I GGWCC 2 cut(s) 172, 205
Eco72I CACGTG 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 387, 465
ErhI CCWWGG 2 cut(s) 387, 465
FaeI CATG 2 cut(s) 469, 558
FaiI YATR 3 cut(s) 467, 477, 556
FaqI GGGAC 3 cut(s) 218, 383, 666
FatI CATG 2 cut(s) 465, 554
FauI CCCGC 1 cut(s) 756
Fnu4HI GCNGC 6 cut(s) 32, 42, 115, 193, 393, 761
FokI GGATG 3 cut(s) 148, 401, 527
Fsp4HI GCNGC 6 cut(s) 32, 42, 115, 193, 393, 761
FspBI CTAG 1 cut(s) 801
GluI GCNGC 6 cut(s) 32, 42, 115, 193, 393, 761
GsaI CCCAGC 2 cut(s) 199, 288
HaeIII GGCC 2 cut(s) 228, 386
HapII CCGG 1 cut(s) 35
Hin1I GRCGYC 2 cut(s) 524, 537
Hin1II CATG 2 cut(s) 469, 558
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 6 cut(s) 10, 149, 154, 352, 491, 670
HpaII CCGG 1 cut(s) 35
HphI GGTGA 3 cut(s) 227, 403, 545
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 4 cut(s) 60, 159, 309, 496
Hpy188III TCNNGA 6 cut(s) 183, 279, 356, 418, 481, 664
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 6 cut(s) 122, 134, 184, 221, 266, 269
HpyCH4III ACNGT 2 cut(s) 122, 430
HpyCH4IV ACGT 4 cut(s) 96, 179, 524, 537
HpyCH4V TGCA 1 cut(s) 648
HpyF10VI GCNNNNNNNGC 4 cut(s) 114, 294, 392, 750
HpyF3I CTNAG 2 cut(s) 14, 339
HpySE526I ACGT 4 cut(s) 96, 179, 524, 537
Hsp92I GRCGYC 2 cut(s) 524, 537
Hsp92II CATG 2 cut(s) 469, 558
KflI GGGWCCC 1 cut(s) 205
Kzo9I GATC 2 cut(s) 75, 159
LmnI GCTCC 2 cut(s) 472, 496
Lsp1109I GCAGC 3 cut(s) 53, 179, 379
MaeI CTAG 1 cut(s) 801
MaeII ACGT 4 cut(s) 96, 179, 524, 537
MaeIII GTNAC 6 cut(s) 17, 99, 122, 409, 520, 533
MalI GATC 2 cut(s) 77, 161
MboI GATC 2 cut(s) 75, 159
MboII GAAGA 4 cut(s) 267, 496, 679, 799
MfeI CAATTG 1 cut(s) 719
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 3 cut(s) 230, 705, 719
MlyI GAGTC 3 cut(s) 19, 158, 679
MmeI TCCRAC 1 cut(s) 287
MspI CCGG 1 cut(s) 35
MunI CAATTG 1 cut(s) 719
Mva1269I GAATGC 1 cut(s) 648
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 4 cut(s) 114, 294, 392, 750
NcoI CCATGG 1 cut(s) 465
NdeII GATC 2 cut(s) 75, 159
NlaIII CATG 2 cut(s) 469, 558
NlaIV GGNNCC 2 cut(s) 206, 207
NmuCI GTSAC 4 cut(s) 99, 409, 520, 533
PaeR7I CTCGAG 1 cut(s) 7
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 648
PfeI GAWTC 3 cut(s) 154, 352, 491
PflMI CCANNNNNTGG 1 cut(s) 466
PkrI GCNGC 6 cut(s) 33, 43, 116, 194, 394, 762
PleI GAGTC 3 cut(s) 18, 157, 678
PmaCI CACGTG 1 cut(s) 97
PmlI CACGTG 1 cut(s) 97
PpsI GAGTC 3 cut(s) 18, 157, 678
Ppu21I YACGTR 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 205
Psp5II RGGWCCY 1 cut(s) 205
PspCI CACGTG 1 cut(s) 97
PspFI CCCAGC 2 cut(s) 195, 284
PspN4I GGNNCC 2 cut(s) 206, 207
PspPI GGNCC 2 cut(s) 172, 205
PspPPI RGGWCCY 1 cut(s) 205
PspXI VCTCGAGB 1 cut(s) 7
SatI GCNGC 6 cut(s) 32, 42, 115, 193, 393, 761
Sau3AI GATC 2 cut(s) 75, 159
Sau96I GGNCC 2 cut(s) 172, 205
SchI GAGTC 3 cut(s) 19, 158, 679
SduI GDGCHC 1 cut(s) 378
Sfr274I CTCGAG 1 cut(s) 7
SinI GGWCC 2 cut(s) 172, 205
SlaI CTCGAG 1 cut(s) 7
SmlI CTYRAG 2 cut(s) 7, 662
SmoI CTYRAG 2 cut(s) 7, 662
Sse9I AATT 3 cut(s) 230, 705, 719
SsiI CCGC 7 cut(s) 29, 32, 114, 603, 751, 760, 763
SspMI CTAG 1 cut(s) 801
StyI CCWWGG 2 cut(s) 387, 465
TaaI ACNGT 2 cut(s) 122, 430
TaiI ACGT 4 cut(s) 99, 182, 527, 540
TaqI TCGA 6 cut(s) 8, 152, 182, 378, 425, 441
TaqII GACCGA 1 cut(s) 503
TasI AATT 3 cut(s) 230, 705, 719
TauI GCSGC 3 cut(s) 34, 117, 763
TfiI GAWTC 3 cut(s) 154, 352, 491
TseFI GTSAC 4 cut(s) 99, 409, 520, 533
TseI GCWGC 3 cut(s) 41, 192, 392
Tsp45I GTSAC 4 cut(s) 99, 409, 520, 533
TspDTI ATGAA 1 cut(s) 424
Van91I CCANNNNNTGG 1 cut(s) 466
VpaK11BI GGWCC 2 cut(s) 172, 205
XcmI CCANNNNNNNNNTGG 1 cut(s) 596
XhoI CTCGAG 1 cut(s) 7
XspI CTAG 1 cut(s) 801
ZraI GACGTC 2 cut(s) 525, 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.