Rroxscaffold_2G00128480

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019867
Physical Location & Seq
Reverse (-)
64267943 .. 64268749
807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00128480.1

Sequence Viewer

Length: 807 bp
ATGCCCTCGAGTCTCAGTTACAGCCTACCGCCGCCGGAGGCAGCAGGTCGGCGTTCTCCGAGCAAGCAGAGGCAGATCAAATATCTTTACAGCCACGTGTCACAGGGCGAGGGCGGCGACGGTTACGGCGACGGGATGATGTCAAAAGAGTCGATTCAGATCAACTTTGTGGTCCAAACGTCGAGAGGAAGCTGCTGGGTTGGGGACCCAAACGCCGTCGTTTACGGCCAATTTCACCTCAAAGGTCGCTCAACCATCAACGACGACGACTTGTCTTCAAGACCCAGCCTTTCAAGCAAACTGGGTCGGAAGCTTTCGGGGTGGGGTGTGCCTCAACCTCAGCAGGCAACGATTCTTGAACAGGTGTTTCGGGTGCTCGAAAAGGCCAAGGCTGCCGTCCCCGTAGCTGTCACCATCCAGAAATCGACTGTGTTCATTTTCGATAGTTTGAAAGACCACCAAACCATGGTGGAGCATATCAAGAAGACCGATTCGGAGCATTTTGAGGGTTTGGGGAAAGTGACGTCAGTTGGTGACGTCATCCCTCCTCCATCATGCTCTATTTGTTTGGAGGGCGTTTCACAAGCCCAAGCTCAAAGTGGCGGTGTTGCTCGCTTGCCCTGTTCCCACATTTATCACAAAAACTGCATTCTCCCGTGGCTCAAGAAGAGTCATTTGTGTCCCATTTGCCGACACCCTGACCCAATTTCCCAAGCCCAATTGGGCAAACCCTCAAAACCCCGCCTCCTCCGCGTAGGGCGGCGGGGGTGGTGGTCTCGCTTGTGGAAGAGGTTTAGGACTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

268

Amino Acids

29.61

Weight (kDa)

9.75

Isoelectric Point (pI)

55.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 185 - 231 9.1e-13 Ring finger domain
zf-RING_11 PF17123 186 - 217 1.1e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 186 - 230 8.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 186 - 230 9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_Vps41 PF23555 186 - 230 2.2e-06 Vps41 C-terminal RING finger domain
zf-RING_UBOX PF13445 186 - 228 2.9e-06 RING-type zinc-finger
zf-rbx1 PF12678 201 - 231 5.3e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019867)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0115561
rosa_roxburghii Rroxscaffold_2G00127650 Rroxscaffold_2G00127730 Rroxscaffold_2G00128480 Rroxscaffold_2G00128570
rosa_samantha Rh2BG266200 Rh2CG260600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 527, 540
Acc36I ACCTGC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 466
AccII CGCG 1 cut(s) 753
AciI CCGC 8 cut(s) 29, 32, 114, 603, 742, 751, 760, 763
AcoI YGGCCR 1 cut(s) 226
AcvI CACGTG 1 cut(s) 97
AcyI GRCGYC 2 cut(s) 524, 537
AfiI CCNNNNNNNGG 1 cut(s) 466
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 4 cut(s) 279, 294, 359, 451
AhdI GACNNNNNGTC 1 cut(s) 271
AhlI ACTAGT 1 cut(s) 800
AluBI AGCT 4 cut(s) 192, 313, 407, 593
AluI AGCT 4 cut(s) 192, 313, 407, 593
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 2 cut(s) 17, 780
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 2 cut(s) 226, 384
ApeKI GCWGC 3 cut(s) 41, 192, 392
AspS9I GGNCC 2 cut(s) 172, 205
AsuHPI GGTGA 3 cut(s) 227, 403, 545
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 2 cut(s) 172, 205
BbrPI CACGTG 1 cut(s) 97
BbsI GAAGAC 2 cut(s) 267, 491
Bbv12I GWGCWC 1 cut(s) 378
BbvCI CCTCAGC 1 cut(s) 339
BbvI GCAGC 3 cut(s) 53, 179, 379
BccI CCATC 3 cut(s) 263, 422, 559
BceAI ACGGC 4 cut(s) 142, 200, 241, 380
BcoDI GTCTC 2 cut(s) 17, 780
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 1 cut(s) 801
BfuAI ACCTGC 1 cut(s) 35
BisI GCNGC 6 cut(s) 32, 42, 115, 193, 393, 761
BlsI GCNGC 6 cut(s) 33, 43, 116, 194, 394, 762
Bme18I GGWCC 2 cut(s) 172, 205
BmeRI GACNNNNNGTC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 172, 205
BmiI GGNNCC 2 cut(s) 206, 207
BmrI ACTGGG 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 311
BpiI GAAGAC 2 cut(s) 267, 491
Bpu10I CCTNAGC 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 647
BsaAI YACGTR 1 cut(s) 97
BsaBI GATNNNNATC 1 cut(s) 158
BsaHI GRCGYC 2 cut(s) 524, 537
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 3 cut(s) 387, 465, 656
BsaXI ACNNNNNCTCC 2 cut(s) 729, 759
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse1I ACTGG 1 cut(s) 306
Bse8I GATNNNNATC 1 cut(s) 158
BseDI CCNNGG 3 cut(s) 387, 465, 656
BseGI GGATG 3 cut(s) 141, 414, 540
BseJI GATNNNNATC 1 cut(s) 158
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMII CTCAG 2 cut(s) 28, 353
BseNI ACTGG 1 cut(s) 306
BseRI GAGGAG 2 cut(s) 537, 737
BseXI GCAGC 3 cut(s) 53, 179, 379
BseYI CCCAGC 2 cut(s) 195, 284
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 2 cut(s) 228, 386
BsiHKAI GWGCWC 1 cut(s) 378
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 218, 383, 666
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 2 cut(s) 17, 780
BsmFI GGGAC 3 cut(s) 218, 383, 666
BsmI GAATGC 1 cut(s) 648
BsnI GGCC 2 cut(s) 228, 386
Bso31I GGTCTC 1 cut(s) 780
BsoBI CYCGRG 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 2 cut(s) 75, 159
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 8 cut(s) 29, 32, 114, 603, 742, 751, 760, 763
BspANI GGCC 2 cut(s) 228, 386
BspCNI CTCAG 2 cut(s) 27, 352
BspFNI CGCG 1 cut(s) 753
BspLI GGNNCC 2 cut(s) 206, 207
BspMI ACCTGC 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 780
BsrI ACTGG 1 cut(s) 306
BssECI CCNNGG 3 cut(s) 387, 465, 656
BssMI GATC 2 cut(s) 75, 159
BssNI GRCGYC 2 cut(s) 524, 537
BssT1I CCWWGG 2 cut(s) 387, 465
Bst4CI ACNGT 2 cut(s) 122, 430
Bst6I CTCTTC 2 cut(s) 662, 782
BstACI GRCGYC 2 cut(s) 524, 537
BstBAI YACGTR 1 cut(s) 97
BstC8I GCNNGC 4 cut(s) 65, 345, 613, 617
BstDEI CTNAG 2 cut(s) 14, 339
BstDSI CCRYGG 2 cut(s) 465, 656
BstF5I GGATG 3 cut(s) 141, 414, 540
BstFNI CGCG 1 cut(s) 753
BstKTI GATC 2 cut(s) 78, 162
BstMAI GTCTC 2 cut(s) 17, 780
BstMBI GATC 2 cut(s) 75, 159
BstMWI GCNNNNNNNGC 4 cut(s) 114, 294, 392, 750
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 3 cut(s) 53, 179, 379
BstV2I GAAGAC 2 cut(s) 267, 491
BsuRI GGCC 2 cut(s) 228, 386
BtgI CCRYGG 2 cut(s) 465, 656
BtsCI GGATG 3 cut(s) 141, 414, 540
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 4 cut(s) 65, 345, 613, 617
Cfr13I GGNCC 2 cut(s) 172, 205
CviAII CATG 2 cut(s) 466, 555
DdeI CTNAG 2 cut(s) 14, 339
DpnI GATC 2 cut(s) 77, 161
DpnII GATC 2 cut(s) 75, 159
DriI GACNNNNNGTC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 662, 782
Eam1105I GACNNNNNGTC 1 cut(s) 271
EarI CTCTTC 2 cut(s) 662, 782
Eco130I CCWWGG 2 cut(s) 387, 465
Eco31I GGTCTC 1 cut(s) 780
Eco47I GGWCC 2 cut(s) 172, 205
Eco72I CACGTG 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 387, 465
ErhI CCWWGG 2 cut(s) 387, 465
FaeI CATG 2 cut(s) 469, 558
FaiI YATR 3 cut(s) 467, 477, 556
FaqI GGGAC 3 cut(s) 218, 383, 666
FatI CATG 2 cut(s) 465, 554
FauI CCCGC 2 cut(s) 749, 756
Fnu4HI GCNGC 6 cut(s) 32, 42, 115, 193, 393, 761
FokI GGATG 3 cut(s) 148, 401, 527
Fsp4HI GCNGC 6 cut(s) 32, 42, 115, 193, 393, 761
FspBI CTAG 1 cut(s) 801
GluI GCNGC 6 cut(s) 32, 42, 115, 193, 393, 761
GsaI CCCAGC 2 cut(s) 199, 288
HaeIII GGCC 2 cut(s) 228, 386
HapII CCGG 1 cut(s) 35
Hin1I GRCGYC 2 cut(s) 524, 537
Hin1II CATG 2 cut(s) 469, 558
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 6 cut(s) 10, 149, 154, 352, 491, 670
HpaII CCGG 1 cut(s) 35
HphI GGTGA 3 cut(s) 227, 403, 545
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 4 cut(s) 60, 159, 309, 496
Hpy188III TCNNGA 6 cut(s) 183, 279, 356, 418, 481, 664
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 6 cut(s) 122, 134, 184, 221, 266, 269
HpyCH4III ACNGT 2 cut(s) 122, 430
HpyCH4IV ACGT 4 cut(s) 96, 179, 524, 537
HpyCH4V TGCA 1 cut(s) 648
HpyF10VI GCNNNNNNNGC 4 cut(s) 114, 294, 392, 750
HpyF3I CTNAG 2 cut(s) 14, 339
HpySE526I ACGT 4 cut(s) 96, 179, 524, 537
Hsp92I GRCGYC 2 cut(s) 524, 537
Hsp92II CATG 2 cut(s) 469, 558
KflI GGGWCCC 1 cut(s) 205
Kzo9I GATC 2 cut(s) 75, 159
LmnI GCTCC 2 cut(s) 472, 496
Lsp1109I GCAGC 3 cut(s) 53, 179, 379
MaeI CTAG 1 cut(s) 801
MaeII ACGT 4 cut(s) 96, 179, 524, 537
MaeIII GTNAC 6 cut(s) 17, 99, 122, 409, 520, 533
MalI GATC 2 cut(s) 77, 161
MboI GATC 2 cut(s) 75, 159
MboII GAAGA 4 cut(s) 267, 496, 679, 799
MfeI CAATTG 1 cut(s) 719
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 3 cut(s) 230, 705, 719
MlyI GAGTC 3 cut(s) 19, 158, 679
MmeI TCCRAC 1 cut(s) 287
MspI CCGG 1 cut(s) 35
MunI CAATTG 1 cut(s) 719
Mva1269I GAATGC 1 cut(s) 648
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 4 cut(s) 114, 294, 392, 750
NcoI CCATGG 1 cut(s) 465
NdeII GATC 2 cut(s) 75, 159
NlaIII CATG 2 cut(s) 469, 558
NlaIV GGNNCC 2 cut(s) 206, 207
NmuCI GTSAC 4 cut(s) 99, 409, 520, 533
PaeR7I CTCGAG 1 cut(s) 7
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 648
PfeI GAWTC 3 cut(s) 154, 352, 491
PflMI CCANNNNNTGG 1 cut(s) 466
PkrI GCNGC 6 cut(s) 33, 43, 116, 194, 394, 762
PleI GAGTC 3 cut(s) 18, 157, 678
PmaCI CACGTG 1 cut(s) 97
PmlI CACGTG 1 cut(s) 97
PpsI GAGTC 3 cut(s) 18, 157, 678
Ppu21I YACGTR 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 205
Psp5II RGGWCCY 1 cut(s) 205
PspCI CACGTG 1 cut(s) 97
PspFI CCCAGC 2 cut(s) 195, 284
PspN4I GGNNCC 2 cut(s) 206, 207
PspPI GGNCC 2 cut(s) 172, 205
PspPPI RGGWCCY 1 cut(s) 205
PspXI VCTCGAGB 1 cut(s) 7
SatI GCNGC 6 cut(s) 32, 42, 115, 193, 393, 761
Sau3AI GATC 2 cut(s) 75, 159
Sau96I GGNCC 2 cut(s) 172, 205
SchI GAGTC 3 cut(s) 19, 158, 679
SduI GDGCHC 1 cut(s) 378
Sfr274I CTCGAG 1 cut(s) 7
SinI GGWCC 2 cut(s) 172, 205
SlaI CTCGAG 1 cut(s) 7
SmlI CTYRAG 2 cut(s) 7, 662
SmoI CTYRAG 2 cut(s) 7, 662
SpeI ACTAGT 1 cut(s) 800
Sse9I AATT 3 cut(s) 230, 705, 719
SsiI CCGC 8 cut(s) 29, 32, 114, 603, 742, 751, 760, 763
SspMI CTAG 1 cut(s) 801
StyI CCWWGG 2 cut(s) 387, 465
TaaI ACNGT 2 cut(s) 122, 430
TaiI ACGT 4 cut(s) 99, 182, 527, 540
TaqI TCGA 6 cut(s) 8, 152, 182, 378, 425, 441
TaqII GACCGA 1 cut(s) 503
TasI AATT 3 cut(s) 230, 705, 719
TauI GCSGC 3 cut(s) 34, 117, 763
TfiI GAWTC 3 cut(s) 154, 352, 491
TseFI GTSAC 4 cut(s) 99, 409, 520, 533
TseI GCWGC 3 cut(s) 41, 192, 392
Tsp45I GTSAC 4 cut(s) 99, 409, 520, 533
TspDTI ATGAA 1 cut(s) 424
Van91I CCANNNNNTGG 1 cut(s) 466
VpaK11BI GGWCC 2 cut(s) 172, 205
XcmI CCANNNNNNNNNTGG 1 cut(s) 596
XhoI CTCGAG 1 cut(s) 7
XspI CTAG 1 cut(s) 801
ZraI GACGTC 2 cut(s) 525, 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.