Rroxscaffold_2G00132540

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0021671
Physical Location & Seq
Reverse (-)
69546529 .. 69569526
22998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132540.1

Sequence Viewer

Length: 495 bp
ATGGAGCCCTCGTTGTCCTTAAACATGTCATCTTCACACTGGAGAACTGTAAGAGCAAGATCGGAAAAAGCGCAGCGCAAGGAGCAGCCCGGACTTTCCTTAGCCGAGCCCAAGCACAAGAAGCAGCCCAAAAGAAAGAAAGAGGAGGTGCGGCCTAGCAAAGATAAAAAAAGAGAAAGAAAAGTGAGGCGGGCCCAGAAGAAAAAAAGAAAAGAAAAAGGGCGAGGTGGGCCCATTGCCGCCTTTGGGGGGCTTTGTGATGTGGGGAAGGCTAGGGAGATTCATGGGCATGTGTTGAAATTAGGGGTTTTGGGCGATGTTTACAATGCCAATTCGATTTTGGGGGTTTATTCGAAATATGAAAGGGATTTGGTGTCTTGGAATACAATGATATCTAGATTTTGTCATTCGGGTGATTATGTGAGCTCGTTGGGGATGTTTAGTCGGATGGAAATGATGGAGAAGCTTGGAAATGCACTAAAAAGTCAACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

164

Amino Acids

18.67

Weight (kDa)

10.33

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021671)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0005033.1_g000015
rosa_roxburghii Rroxscaffold_2G00132540
rosa_rugosa Rorug04G0351300
rosa_samantha Rh2BG237300 Rh2CG228500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 151, 190, 240
AfiI CCNNNNNNNGG 2 cut(s) 246, 249
AflIII ACRYGT 1 cut(s) 24
AgsI TTSAA 1 cut(s) 298
AluBI AGCT 2 cut(s) 426, 466
AluI AGCT 2 cut(s) 426, 466
Alw21I GWGCWC 1 cut(s) 428
AoxI GGCC 3 cut(s) 152, 192, 230
ApaI GGGCCC 2 cut(s) 196, 234
ApeKI GCWGC 3 cut(s) 73, 85, 124
AspLEI GCGC 2 cut(s) 73, 78
AspS9I GGNCC 4 cut(s) 192, 193, 230, 231
AsuC2I CCSGG 1 cut(s) 90
AsuHPI GGTGA 1 cut(s) 425
AsuII TTCGAA 1 cut(s) 353
BaeGI GKGCMC 2 cut(s) 196, 234
BanII GRGCYC 5 cut(s) 9, 111, 196, 234, 428
Bbv12I GWGCWC 1 cut(s) 428
BbvI GCAGC 3 cut(s) 85, 97, 136
BccI CCATC 2 cut(s) 442, 451
BcnI CCSGG 1 cut(s) 90
BfaI CTAG 3 cut(s) 156, 273, 396
BisI GCNGC 5 cut(s) 74, 86, 125, 152, 240
BlsI GCNGC 5 cut(s) 75, 87, 126, 153, 241
Bme1390I CCNGG 1 cut(s) 90
BmgT120I GGNCC 4 cut(s) 192, 193, 230, 231
BmiI GGNNCC 3 cut(s) 6, 194, 232
BmrFI CCNGG 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 61
Bpu10I CCTNAGC 1 cut(s) 100
Bpu14I TTCGAA 1 cut(s) 353
BpuMI CCSGG 1 cut(s) 90
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 246, 249
Bse1I ACTGG 1 cut(s) 44
Bse3DI GCAATG 1 cut(s) 234
BseGI GGATG 2 cut(s) 441, 453
BseLI CCNNNNNNNGG 2 cut(s) 246, 249
BseMI GCAATG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 44
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 196, 234
BseXI GCAGC 3 cut(s) 85, 97, 136
BshFI GGCC 3 cut(s) 154, 194, 232
BsiHKAI GWGCWC 1 cut(s) 428
BsiSI CCGG 1 cut(s) 90
BslI CCNNNNNNNGG 2 cut(s) 246, 249
BsnI GGCC 3 cut(s) 154, 194, 232
Bsp119I TTCGAA 1 cut(s) 353
Bsp120I GGGCCC 2 cut(s) 192, 230
Bsp1286I GDGCHC 5 cut(s) 9, 111, 196, 234, 428
Bsp143I GATC 1 cut(s) 59
BspACI CCGC 3 cut(s) 151, 190, 240
BspANI GGCC 3 cut(s) 154, 194, 232
BspLI GGNNCC 3 cut(s) 6, 194, 232
BspT104I TTCGAA 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 234
BsrI ACTGG 1 cut(s) 44
BssMI GATC 1 cut(s) 59
Bst4CI ACNGT 1 cut(s) 49
BstBI TTCGAA 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 2 cut(s) 100, 492
BstF5I GGATG 2 cut(s) 441, 453
BstHHI GCGC 2 cut(s) 73, 78
BstKTI GATC 1 cut(s) 62
BstMBI GATC 1 cut(s) 59
BstMWI GCNNNNNNNGC 3 cut(s) 82, 121, 229
BstNSI RCATGY 2 cut(s) 28, 293
BstSCI CCNGG 1 cut(s) 88
BstSLI GKGCMC 2 cut(s) 196, 234
BstV1I GCAGC 3 cut(s) 85, 97, 136
BsuRI GGCC 3 cut(s) 154, 194, 232
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 2 cut(s) 441, 453
BtsIMutI CAGTG 1 cut(s) 37
Cac8I GCNNGC 1 cut(s) 192
CfoI GCGC 2 cut(s) 73, 78
Cfr13I GGNCC 4 cut(s) 192, 193, 230, 231
CviAII CATG 3 cut(s) 25, 284, 290
DdeI CTNAG 2 cut(s) 100, 492
DpnI GATC 1 cut(s) 61
DpnII GATC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 426
Eco24I GRGCYC 5 cut(s) 9, 111, 196, 234, 428
Eco32I GATATC 1 cut(s) 393
Eco53kI GAGCTC 1 cut(s) 426
EcoICRI GAGCTC 1 cut(s) 426
EcoRV GATATC 1 cut(s) 393
EcoT38I GRGCYC 5 cut(s) 9, 111, 196, 234, 428
FaeI CATG 3 cut(s) 28, 287, 293
FaiI YATR 5 cut(s) 26, 285, 291, 360, 420
FalI AAGNNNNNCTT 1 cut(s) 475
FatI CATG 3 cut(s) 24, 283, 289
FauI CCCGC 1 cut(s) 183
Fnu4HI GCNGC 5 cut(s) 74, 86, 125, 152, 240
FokI GGATG 2 cut(s) 448, 460
FriOI GRGCYC 5 cut(s) 9, 111, 196, 234, 428
Fsp4HI GCNGC 5 cut(s) 74, 86, 125, 152, 240
FspBI CTAG 3 cut(s) 156, 273, 396
GlaI GCGC 2 cut(s) 72, 77
GluI GCNGC 5 cut(s) 74, 86, 125, 152, 240
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 3 cut(s) 154, 194, 232
HapII CCGG 1 cut(s) 90
HhaI GCGC 2 cut(s) 73, 78
Hin1II CATG 3 cut(s) 28, 287, 293
Hin6I GCGC 2 cut(s) 71, 76
HinP1I GCGC 2 cut(s) 71, 76
HincII GTYRAC 1 cut(s) 488
HindII GTYRAC 1 cut(s) 488
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 1 cut(s) 280
HpaII CCGG 1 cut(s) 90
HphI GGTGA 1 cut(s) 425
Hpy166II GTNNAC 2 cut(s) 322, 488
Hpy188I TCNGA 2 cut(s) 64, 447
Hpy188III TCNNGA 1 cut(s) 396
Hpy8I GTNNAC 2 cut(s) 322, 488
HpyAV CCTTC 1 cut(s) 262
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4V TGCA 1 cut(s) 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 121, 229
HpyF3I CTNAG 2 cut(s) 100, 492
Hsp92II CATG 3 cut(s) 28, 287, 293
HspAI GCGC 2 cut(s) 71, 76
Kzo9I GATC 1 cut(s) 59
LmnI GCTCC 2 cut(s) 4, 82
LpnPI CCDG 3 cut(s) 25, 103, 209
Lsp1109I GCAGC 3 cut(s) 85, 97, 136
MaeI CTAG 3 cut(s) 156, 273, 396
MalI GATC 1 cut(s) 61
MboI GATC 1 cut(s) 59
MboII GAAGA 2 cut(s) 24, 211
MhlI GDGCHC 5 cut(s) 9, 111, 196, 234, 428
MluCI AATT 2 cut(s) 299, 331
MmeI TCCRAC 1 cut(s) 425
MnlI CCTC 5 cut(s) 19, 136, 139, 180, 218
MseI TTAA 1 cut(s) 20
MslI CAYNNNNRTG 2 cut(s) 288, 411
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 90
MteI GCGCNGCGC 1 cut(s) 74
MwoI GCNNNNNNNGC 3 cut(s) 82, 121, 229
NciI CCSGG 1 cut(s) 90
NdeII GATC 1 cut(s) 59
NlaIII CATG 3 cut(s) 28, 287, 293
NlaIV GGNNCC 3 cut(s) 6, 194, 232
NmeAIII GCCGAG 1 cut(s) 130
NspI RCATGY 2 cut(s) 28, 293
NspV TTCGAA 1 cut(s) 353
PciI ACATGT 1 cut(s) 24
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 5 cut(s) 75, 87, 126, 153, 241
PscI ACATGT 1 cut(s) 24
Psp124BI GAGCTC 1 cut(s) 428
PspN4I GGNNCC 3 cut(s) 6, 194, 232
PspOMI GGGCCC 2 cut(s) 192, 230
PspPI GGNCC 4 cut(s) 192, 193, 230, 231
RseI CAYNNNNRTG 2 cut(s) 288, 411
SacI GAGCTC 1 cut(s) 428
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 5 cut(s) 74, 86, 125, 152, 240
Sau3AI GATC 1 cut(s) 59
Sau96I GGNCC 4 cut(s) 192, 193, 230, 231
ScrFI CCNGG 1 cut(s) 90
SduI GDGCHC 5 cut(s) 9, 111, 196, 234, 428
SetI ASST 5 cut(s) 150, 229, 428, 468, 493
SfuI TTCGAA 1 cut(s) 353
SmiMI CAYNNNNRTG 2 cut(s) 288, 411
Sse9I AATT 2 cut(s) 299, 331
SsiI CCGC 3 cut(s) 151, 190, 240
SspMI CTAG 3 cut(s) 156, 273, 396
SstI GAGCTC 1 cut(s) 428
StyD4I CCNGG 1 cut(s) 88
TaaI ACNGT 1 cut(s) 49
TaqI TCGA 2 cut(s) 335, 353
TasI AATT 2 cut(s) 299, 331
TauI GCSGC 2 cut(s) 154, 242
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 1 cut(s) 44
TseI GCWGC 3 cut(s) 73, 85, 124
TspDTI ATGAA 2 cut(s) 272, 375
TspRI CASTG 1 cut(s) 44
XbaI TCTAGA 1 cut(s) 395
XceI RCATGY 2 cut(s) 28, 293
XcmI CCANNNNNNNNNTGG 1 cut(s) 337
XspI CTAG 3 cut(s) 156, 273, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.