Rorug04G0351300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001686
Physical Location & Seq
Forward (+)
52332592 .. 52336882
4291 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0351300.1

Sequence Viewer

Length: 648 bp
ATGCAGAGAGAATCCGGTTCGGCTTTGCCGCTCCGAGCAAGACTCATCGACTTCCTCATCCAATCTGCTCAGCGGCTCCAACTTTCTCCGACCGAGAAGTACACCGCCTTGTCACTCTTCGCTCATAGATTCTACCCCCGTTTTATGTCCCAGATGGAACAAGGCAATGACATGGGGAACTGGCTCTTGCAACCCTTGAGAGAGAGCAATTTGCAGCTATTTGCGCTTGTTTCGCTATGGATTTCAAACAAAATACACTCTTCTCCTGCATTGTCCGTGAAGATATTCAAGTCCTTGGGAGACAAATGTATCAGTGAACAACACTTCACCATTCGAGATTATTCTGAAGCGGAGGTGGTCTTAATGCAGGTAGTGAATTTTGAGATTTGTGTGGAAAATGTTGCTTTCGTATACTTTGAAGAGCTTTTACTCCAATTCAAGGGAGTGTCAAAGGTTGGGGAACTAGTGAACTTTGAAGCAGGCATGGACATCATGGATCTACTTTATGAAAAGGAGGAGACAACGATGCTCTATGCTAATCCTCGTTTTCTCGCTGCATCAGTCTTGGTTGCTTCATATGTCATTACAGTTCCCAAACAGAAGTTGGAATTTCCGGTTCTTCCCTGGGTGATGCCGATAACAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

215

Amino Acids

24.71

Weight (kDa)

5.69

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_N PF00134 9 - 129 1.8e-07 Cyclin, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 358
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 411
AciI CCGC 4 cut(s) 29, 73, 105, 350
AclWI GGATC 1 cut(s) 504
AcsI RAATTY 2 cut(s) 376, 608
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 439
AgsI TTSAA 5 cut(s) 246, 289, 419, 439, 476
AhlI ACTAGT 1 cut(s) 463
AjnI CCWGG 1 cut(s) 623
AluBI AGCT 2 cut(s) 217, 424
AluI AGCT 2 cut(s) 217, 424
Alw26I GTCTC 2 cut(s) 294, 512
AlwI GGATC 1 cut(s) 504
ApeKI GCWGC 2 cut(s) 214, 554
ApoI RAATTY 2 cut(s) 376, 608
Asp700I GAANNNNTTC 1 cut(s) 284
AspLEI GCGC 1 cut(s) 226
AsuHPI GGTGA 2 cut(s) 319, 640
BaeI ACNNNNGTAYC 2 cut(s) 292, 325
BarI GAAGNNNNNNTAC 2 cut(s) 411, 443
BbvI GCAGC 2 cut(s) 226, 541
BccI CCATC 1 cut(s) 148
BciT130I CCWGG 1 cut(s) 625
BcoDI GTCTC 2 cut(s) 294, 512
BcuI ACTAGT 1 cut(s) 463
BfaI CTAG 1 cut(s) 464
BfuAI ACCTGC 1 cut(s) 358
BisI GCNGC 4 cut(s) 29, 74, 215, 555
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 4 cut(s) 30, 75, 216, 556
Bme1390I CCNGG 1 cut(s) 625
BmiI GGNNCC 1 cut(s) 77
BmrFI CCNGG 1 cut(s) 625
BmsI GCATC 3 cut(s) 516, 566, 621
BplI GAGNNNNNCTC 2 cut(s) 27, 59
Bpu1102I GCTNAGC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 217
BsaJI CCNNGG 3 cut(s) 294, 623, 624
BsaWI WCCGGW 2 cut(s) 14, 613
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse1I ACTGG 1 cut(s) 185
Bse3DI GCAATG 1 cut(s) 172
BseBI CCWGG 1 cut(s) 625
BseDI CCNNGG 3 cut(s) 294, 623, 624
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMI GCAATG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 185
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 2 cut(s) 226, 541
Bsh1285I CGRYCG 1 cut(s) 93
BsiEI CGRYCG 1 cut(s) 93
BsiSI CCGG 2 cut(s) 15, 614
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 2 cut(s) 294, 512
BsmFI GGGAC 1 cut(s) 133
Bsp143I GATC 1 cut(s) 496
Bsp1720I GCTNAGC 1 cut(s) 69
BspACI CCGC 4 cut(s) 29, 73, 105, 350
BspCNI CTCAG 1 cut(s) 82
BspLI GGNNCC 1 cut(s) 77
BspMI ACCTGC 1 cut(s) 358
BspPI GGATC 1 cut(s) 504
BspQI GCTCTTC 1 cut(s) 414
BsrBI CCGCTC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 172
BsrI ACTGG 1 cut(s) 185
BssECI CCNNGG 3 cut(s) 294, 623, 624
BssMI GATC 1 cut(s) 496
BssNAI GTATAC 1 cut(s) 412
BssT1I CCWWGG 1 cut(s) 294
Bst1107I GTATAC 1 cut(s) 412
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 1 cut(s) 589
Bst6I CTCTTC 3 cut(s) 122, 265, 414
BstC8I GCNNGC 1 cut(s) 481
BstDEI CTNAG 1 cut(s) 69
BstF5I GGATG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 226
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 2 cut(s) 294, 512
BstMBI GATC 1 cut(s) 496
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 2 cut(s) 223, 232
BstNI CCWGG 1 cut(s) 625
BstSCI CCNGG 1 cut(s) 623
BstV1I GCAGC 2 cut(s) 226, 541
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BstZ17I GTATAC 1 cut(s) 412
BtsCI GGATG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 319
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 1 cut(s) 481
CfoI GCGC 1 cut(s) 226
Csp6I GTAC 1 cut(s) 100
CviAII CATG 3 cut(s) 172, 484, 493
CviJI RGCY 5 cut(s) 23, 76, 184, 217, 424
CviKI_1 RGCY 5 cut(s) 23, 76, 184, 217, 424
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 69
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
Eam1104I CTCTTC 3 cut(s) 122, 265, 414
EarI CTCTTC 3 cut(s) 122, 265, 414
Eco130I CCWWGG 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 366
EcoRII CCWGG 1 cut(s) 623
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 3 cut(s) 175, 487, 496
FaqI GGGAC 1 cut(s) 133
FatI CATG 3 cut(s) 171, 483, 492
FauNDI CATATG 1 cut(s) 577
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 4 cut(s) 29, 74, 215, 555
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 4 cut(s) 29, 74, 215, 555
FspBI CTAG 1 cut(s) 464
GlaI GCGC 1 cut(s) 225
GluI GCNGC 4 cut(s) 29, 74, 215, 555
HapII CCGG 2 cut(s) 15, 614
HhaI GCGC 1 cut(s) 226
Hin1II CATG 3 cut(s) 175, 487, 496
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HinfI GANTC 3 cut(s) 11, 42, 129
HpaII CCGG 2 cut(s) 15, 614
HphI GGTGA 2 cut(s) 319, 640
Hpy166II GTNNAC 4 cut(s) 102, 317, 412, 469
Hpy188I TCNGA 3 cut(s) 35, 90, 346
Hpy188III TCNNGA 1 cut(s) 335
Hpy8I GTNNAC 4 cut(s) 102, 317, 412, 469
HpyCH4III ACNGT 1 cut(s) 589
HpyCH4V TGCA 6 cut(s) 4, 190, 214, 269, 367, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 223, 232
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 3 cut(s) 175, 487, 496
HspAI GCGC 1 cut(s) 224
Kzo9I GATC 1 cut(s) 496
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 2 cut(s) 36, 81
LpnPI CCDG 9 cut(s) 28, 164, 166, 279, 353, 465, 610, 627, 637
Lsp1109I GCAGC 2 cut(s) 226, 541
LweI GCATC 3 cut(s) 516, 566, 621
MaeI CTAG 1 cut(s) 464
MaeIII GTNAC 1 cut(s) 111
MalI GATC 1 cut(s) 498
MbiI CCGCTC 1 cut(s) 31
MboI GATC 1 cut(s) 496
MboII GAAGA 5 cut(s) 109, 252, 292, 431, 611
MflI RGATCY 1 cut(s) 496
MluCI AATT 4 cut(s) 208, 376, 434, 608
MlyI GAGTC 1 cut(s) 36
MmeI TCCRAC 3 cut(s) 103, 113, 585
MnlI CCTC 4 cut(s) 65, 346, 508, 552
MroXI GAANNNNTTC 1 cut(s) 284
MseI TTAA 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 73
MspI CCGG 2 cut(s) 15, 614
MspR9I CCNGG 1 cut(s) 625
MvaI CCWGG 1 cut(s) 625
MwoI GCNNNNNNNGC 2 cut(s) 223, 232
NdeI CATATG 1 cut(s) 577
NdeII GATC 1 cut(s) 496
NlaIII CATG 3 cut(s) 175, 487, 496
NlaIV GGNNCC 1 cut(s) 77
NmuCI GTSAC 1 cut(s) 111
PasI CCCWGGG 1 cut(s) 624
PciSI GCTCTTC 1 cut(s) 414
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 2 cut(s) 11, 129
PkrI GCNGC 4 cut(s) 30, 75, 216, 556
PleI GAGTC 1 cut(s) 36
PpsI GAGTC 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 1 cut(s) 77
PsuI RGATCY 1 cut(s) 496
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 1 cut(s) 362
SatI GCNGC 4 cut(s) 29, 74, 215, 555
Sau3AI GATC 1 cut(s) 496
SchI GAGTC 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 625
SetI ASST 6 cut(s) 219, 357, 372, 426, 456, 647
SfaNI GCATC 3 cut(s) 516, 566, 621
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
SpeI ACTAGT 1 cut(s) 463
Sse9I AATT 4 cut(s) 208, 376, 434, 608
SsiI CCGC 4 cut(s) 29, 73, 105, 350
SspMI CTAG 1 cut(s) 464
StyD4I CCNGG 1 cut(s) 623
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 1 cut(s) 589
TaqI TCGA 2 cut(s) 48, 334
TaqII GACCGA 1 cut(s) 107
TasI AATT 4 cut(s) 208, 376, 434, 608
TatI WGTACW 1 cut(s) 99
TauI GCSGC 2 cut(s) 31, 76
TfiI GAWTC 2 cut(s) 11, 129
Tru1I TTAA 1 cut(s) 362
Tru9I TTAA 1 cut(s) 362
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 1 cut(s) 111
TseI GCWGC 2 cut(s) 214, 554
Tsp45I GTSAC 1 cut(s) 111
TspDTI ATGAA 2 cut(s) 522, 564
TspGWI ACGGA 1 cut(s) 265
TspRI CASTG 1 cut(s) 319
XapI RAATTY 2 cut(s) 376, 608
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 411
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.