Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001686

Physical Location & Seq

Genomic Coordinates Reverse (-)

6119171 .. 6121758

Gene Structure 2588 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1088900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGAGCATTGAGGGAGAGAGAGAGAGAGAAAGAGATAAGATGAGCATTGAGGGTTTTCTGCCATTTATTGGAATGGTGATCGCGATGATGGTTCAAACTGGGAGTATGGTGGTAAACAAAGCAGCCATGTCCAAAGGGACCAACGCCTACATAATCGTCGTCTATGCCAACGCCGTCTCCGCCCTCATTCTTCTCCCTTCCGCTCTCATCTTCCACAGATCGGAGCGCCCTCCCCTTACATTCTCCATCCTCTGCAAAATCTTCTTGCTAGCACTGTTTTGTTGTACATCGCAAATATTTGGAAATATTGGCATAGAATACAGCTCTCCCACACTTAGCACGGCCATGCTTAACCTCATTCCAGCTTTTACTTTCATACTTGCCATCATTTTCAGGATGGAAAGAGTTTACTGGAGAAGCTCAAGCAGCCAAGCAAAAGTTTTGGGAACCATAGTATCAATCACAGGAGCATTTGTTGTAACATTTTACAAAGGCCTACCAATCATACGGCTATCATCATCACTTGCTAGTTTACTCAACCAACTGATCCTTTCCTCATCGAAATCAAACTGGATCCTTGGAGGGTTTTTTCTTGCAGCTGACGCCTTGTCAACTTCTTTATGGTACATTCTGCAGGCAACAATCGTTAAGAAGTACCCTGCAATTGTGTTCATTGTCTTTTTCCAATGCTTATTCGCGACCATGCAGTCTGCAGCATTTACTTTAATTGCAGTTAGAGGTGCAAGTGCCTGGGAAATAAGGCTGGATGTGGGGATCATTGCTATTTTATACACAGGAATTGTTTCGACGGTTCTCCGATACAGTTTAGTCACCTGGTGTGTGAAGAAGGCTGGAGCTTTTTATTGTTCTATGTTTAAGCCTCTTGGAATCATTTTTGGTGTGATCATGGGTGTCATATTTTTGGGAGATTCAGTCTATCTTGGAAGCTTAGTTGGTGCAGCAATAATTGTTACTGGATTTTATGCCATGATGTGGGGAAAGGCCAAAGAAGAGAAGCTGATCGAAATCGAATCACTAGACCAAAACAAGCTCCCTTTGCTACAAAACGGCTTACAAGAGAAGCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009987 GO:0010033 GO:0016020 GO:0016021 GO:0023052 GO:0031224 GO:0031226 GO:0032870 GO:0042221 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

363

Amino Acids

39.78

Weight (kDa)

9.31

Isoelectric Point (pI)

44.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	23 - 161	5.1e-08	EamA-like transporter family
EamA	PF00892	194 - 330	6.7e-10	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001686)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g34870 FvH4_4g34870 FvH4_4g34870 FvH4_4g34870 FvH4_4g34870 FvH4_4g34870 FvH4_4g34870 FvH4_4g34870
malus_domestica	MD13G1088500.v1.1 MD13G1088700.v1.1 MD16G1088800.v1.1 MD16G1088900.v1.1
prunus_persica	Prupe.1G257200_v2.0.a1 Prupe.1G257300_v2.0.a1 Prupe.1G257400_v2.0.a1 Prupe.1G257500_v2.0.a1 Prupe.1G257600_v2.0.a1 Prupe.1G257700_v2.0.a1
pyrus_communis	pycom13g07640 pycom13g07650 pycom16g07610
rosa_chinensis	RchiOBHm_Chr4g0411271 RchiOBHm_Chr4g0444271 RchiOBHm_Chr4g0444291 RchiOBHm_Chr4g0444301
rosa_laevigata	RLG00000005843
rosa_multiflora	Rmu_sc0002841.1_g000015
rosa_roxburghii	Rroxscaffold_5G00384840 Rroxscaffold_5G00384850
rosa_rugosa	Rorug03G0072000 Rorug03G0072100 Rorug04G0351100 Rorug04G0351200 Rorug04G0351300 Rorug04G0351300 Rorug04G0351300 Rorug04G0351300 Rorug07G0109600
rosa_samantha	Rh1BG171600 Rh4AG412000 Rh4BG070500 Rh4BG423500 Rh4CG078800 Rh4DG418700 Rh4DG418800
rosa_wichuraiana	Rw2G039380 Rw4G013770 Rw4G035390 Rw5G040980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	706
AccB7I	CCANNNNNTGG	2 cut(s)	68, 993
AccBSI	CCGCTC	1 cut(s)	203
AccII	CGCG	2 cut(s)	83, 698
AciI	CCGC	2 cut(s)	180, 201
AclWI	GGATC	4 cut(s)	541, 568, 581, 782
AcoI	YGGCCR	1 cut(s)	342
AcyI	GRCGYC	1 cut(s)	603
AdeI	CACNNNGTG	1 cut(s)	837
AfaI	GTAC	3 cut(s)	286, 626, 656
AfiI	CCNNNNNNNGG	3 cut(s)	68, 220, 993
AgsI	TTSAA	1 cut(s)	95
AhdI	GACNNNNNGTC	1 cut(s)	607
AjnI	CCWGG	2 cut(s)	749, 833
AluBI	AGCT	8 cut(s)	324, 365, 420, 599, 857, 948, 1018, 1051
AluI	AGCT	8 cut(s)	324, 365, 420, 599, 857, 948, 1018, 1051
Alw26I	GTCTC	1 cut(s)	181
AlwI	GGATC	4 cut(s)	541, 568, 581, 782
AoxI	GGCC	3 cut(s)	342, 493, 1002
ApeKI	GCWGC	5 cut(s)	122, 426, 596, 713, 959
Asp700I	GAANNNNTTC	1 cut(s)	802
AspLEI	GCGC	1 cut(s)	228
AspS9I	GGNCC	1 cut(s)	138
AsuHPI	GGTGA	2 cut(s)	88, 823
AsuNHI	GCTAGC	1 cut(s)	268
AvaII	GGWCC	1 cut(s)	138
BaeI	ACNNNNGTAYC	2 cut(s)	438, 471
BamHI	GGATCC	1 cut(s)	573
BbvI	GCAGC	5 cut(s)	134, 438, 608, 725, 971
BccI	CCATC	4 cut(s)	82, 254, 391, 392
BceAI	ACGGC	4 cut(s)	158, 357, 524, 1084
BciT130I	CCWGG	2 cut(s)	751, 835
BclI	TGATCA	1 cut(s)	903
BcoDI	GTCTC	1 cut(s)	181
BfaI	CTAG	3 cut(s)	269, 528, 1037
BfmI	CTRYAG	2 cut(s)	632, 711
BfoI	RGCGCY	1 cut(s)	229
BisI	GCNGC	5 cut(s)	123, 427, 597, 714, 960
BlsI	GCNGC	5 cut(s)	124, 428, 598, 715, 961
Bme1390I	CCNGG	2 cut(s)	751, 835
Bme18I	GGWCC	1 cut(s)	138
BmeRI	GACNNNNNGTC	1 cut(s)	607
BmgT120I	GGNCC	1 cut(s)	138
BmiI	GGNNCC	3 cut(s)	139, 448, 575
BmrFI	CCNGG	2 cut(s)	751, 835
BmrI	ACTGGG	1 cut(s)	108
BmtI	GCTAGC	1 cut(s)	272
BmuI	ACTGGG	1 cut(s)	108
BpmI	CTGGAG	2 cut(s)	433, 873
BpuEI	CTTGAG	1 cut(s)	406
BsaBI	GATNNNNATC	1 cut(s)	1025
BsaHI	GRCGYC	1 cut(s)	603
BsaJI	CCNNGG	2 cut(s)	577, 750
BsaXI	ACNNNNNCTCC	6 cut(s)	161, 191, 459, 489, 918, 948
Bsc4I	CCNNNNNNNGG	3 cut(s)	68, 220, 993
Bse1I	ACTGG	4 cut(s)	103, 416, 575, 979
Bse3DI	GCAATG	1 cut(s)	777
Bse8I	GATNNNNATC	1 cut(s)	1025
BseBI	CCWGG	2 cut(s)	751, 835
BseDI	CCNNGG	2 cut(s)	577, 750
BseGI	GGATG	3 cut(s)	246, 402, 772
BseJI	GATNNNNATC	1 cut(s)	1025
BseLI	CCNNNNNNNGG	3 cut(s)	68, 220, 993
BseMI	GCAATG	1 cut(s)	777
BseNI	ACTGG	4 cut(s)	103, 416, 575, 979
BseXI	GCAGC	5 cut(s)	134, 438, 608, 725, 971
BsgI	GTGCAG	1 cut(s)	978
Bsh1236I	CGCG	2 cut(s)	83, 698
BshFI	GGCC	3 cut(s)	344, 495, 1004
BslFI	GGGAC	1 cut(s)	151
BslI	CCNNNNNNNGG	3 cut(s)	68, 220, 993
BsmAI	GTCTC	1 cut(s)	181
BsmBI	CGTCTC	1 cut(s)	181
BsmFI	GGGAC	1 cut(s)	151
BsnI	GGCC	3 cut(s)	344, 495, 1004
Bsp1407I	TGTACA	1 cut(s)	284
Bsp143I	GATC	7 cut(s)	78, 218, 546, 573, 774, 903, 1020
Bsp68I	TCGCGA	2 cut(s)	83, 698
BspACI	CCGC	2 cut(s)	180, 201
BspANI	GGCC	3 cut(s)	344, 495, 1004
BspFNI	CGCG	2 cut(s)	83, 698
BspLI	GGNNCC	3 cut(s)	139, 448, 575
BspMAI	CTGCAG	2 cut(s)	636, 715
BspOI	GCTAGC	1 cut(s)	272
BspPI	GGATC	4 cut(s)	541, 568, 581, 782
BsrBI	CCGCTC	1 cut(s)	203
BsrDI	GCAATG	1 cut(s)	777
BsrGI	TGTACA	1 cut(s)	284
BsrI	ACTGG	4 cut(s)	103, 416, 575, 979
BssECI	CCNNGG	2 cut(s)	577, 750
BssMI	GATC	7 cut(s)	78, 218, 546, 573, 774, 903, 1020
BssNI	GRCGYC	1 cut(s)	603
BssT1I	CCWWGG	1 cut(s)	577
Bst2UI	CCWGG	2 cut(s)	751, 835
Bst4CI	ACNGT	3 cut(s)	276, 811, 824
Bst6I	CTCTTC	1 cut(s)	1005
BstACI	GRCGYC	1 cut(s)	603
BstAUI	TGTACA	1 cut(s)	284
BstC8I	GCNNGC	2 cut(s)	270, 636
BstDEI	CTNAG	2 cut(s)	335, 949
BstF5I	GGATG	3 cut(s)	246, 402, 772
BstFNI	CGCG	2 cut(s)	83, 698
BstH2I	RGCGCY	1 cut(s)	229
BstHHI	GCGC	1 cut(s)	228
BstKTI	GATC	7 cut(s)	81, 221, 549, 576, 777, 906, 1023
BstMAI	GTCTC	1 cut(s)	181
BstMBI	GATC	7 cut(s)	78, 218, 546, 573, 774, 903, 1020
BstMWI	GCNNNNNNNGC	4 cut(s)	179, 426, 602, 1057
BstNI	CCWGG	2 cut(s)	751, 835
BstSCI	CCNGG	2 cut(s)	749, 833
BstSFI	CTRYAG	2 cut(s)	632, 711
BstUI	CGCG	2 cut(s)	83, 698
BstV1I	GCAGC	5 cut(s)	134, 438, 608, 725, 971
BstX2I	RGATCY	1 cut(s)	573
BstYI	RGATCY	1 cut(s)	573
BsuRI	GGCC	3 cut(s)	344, 495, 1004
BtgZI	GCGATG	2 cut(s)	98, 273
BtsCI	GGATG	3 cut(s)	246, 402, 772
BtsIMutI	CAGTG	1 cut(s)	272
BtuMI	TCGCGA	2 cut(s)	83, 698
Cac8I	GCNNGC	2 cut(s)	270, 636
CfoI	GCGC	1 cut(s)	228
Cfr13I	GGNCC	1 cut(s)	138
CseI	GACGC	1 cut(s)	611
CsiI	ACCWGGT	1 cut(s)	833
Csp6I	GTAC	3 cut(s)	285, 625, 655
CviAII	CATG	5 cut(s)	127, 346, 703, 907, 988
CviQI	GTAC	3 cut(s)	285, 625, 655
DdeI	CTNAG	2 cut(s)	335, 949
DpnI	GATC	7 cut(s)	80, 220, 548, 575, 776, 905, 1022
DpnII	GATC	7 cut(s)	78, 218, 546, 573, 774, 903, 1020
DraIII	CACNNNGTG	1 cut(s)	837
DrdI	GACNNNNNNGTC	1 cut(s)	706
DriI	GACNNNNNGTC	1 cut(s)	607
DseDI	GACNNNNNNGTC	1 cut(s)	706
EaeI	YGGCCR	1 cut(s)	342
Eam1104I	CTCTTC	1 cut(s)	1005
Eam1105I	GACNNNNNGTC	1 cut(s)	607
EarI	CTCTTC	1 cut(s)	1005
EciI	GGCGGA	1 cut(s)	169
Eco130I	CCWWGG	1 cut(s)	577
Eco147I	AGGCCT	1 cut(s)	495
Eco47I	GGWCC	1 cut(s)	138
EcoRII	CCWGG	2 cut(s)	749, 833
EcoT14I	CCWWGG	1 cut(s)	577
ErhI	CCWWGG	1 cut(s)	577
Esp3I	CGTCTC	1 cut(s)	181
FaeI	CATG	5 cut(s)	130, 349, 706, 910, 991
FaqI	GGGAC	1 cut(s)	151
FatI	CATG	5 cut(s)	126, 345, 702, 906, 987
FbaI	TGATCA	1 cut(s)	903
Fnu4HI	GCNGC	5 cut(s)	123, 427, 597, 714, 960
FokI	GGATG	3 cut(s)	233, 409, 779
Fsp4HI	GCNGC	5 cut(s)	123, 427, 597, 714, 960
FspBI	CTAG	3 cut(s)	269, 528, 1037
GlaI	GCGC	1 cut(s)	227
GluI	GCNGC	5 cut(s)	123, 427, 597, 714, 960
GsuI	CTGGAG	2 cut(s)	433, 873
HaeII	RGCGCY	1 cut(s)	229
HaeIII	GGCC	3 cut(s)	344, 495, 1004
HgaI	GACGC	1 cut(s)	611
HhaI	GCGC	1 cut(s)	228
Hin1I	GRCGYC	1 cut(s)	603
Hin1II	CATG	5 cut(s)	130, 349, 706, 910, 991
Hin6I	GCGC	1 cut(s)	226
HinP1I	GCGC	1 cut(s)	226
HincII	GTYRAC	1 cut(s)	612
HindII	GTYRAC	1 cut(s)	612
HindIII	AAGCTT	1 cut(s)	946
HinfI	GANTC	3 cut(s)	888, 929, 1031
HphI	GGTGA	2 cut(s)	88, 823
Hpy166II	GTNNAC	4 cut(s)	115, 409, 533, 612
Hpy188I	TCNGA	2 cut(s)	223, 818
Hpy188III	TCNNGA	3 cut(s)	82, 394, 697
Hpy8I	GTNNAC	4 cut(s)	115, 409, 533, 612
Hpy99I	CGWCG	2 cut(s)	161, 811
HpyAV	CCTTC	2 cut(s)	207, 841
HpyCH4III	ACNGT	3 cut(s)	276, 811, 824
HpyCH4V	TGCA	9 cut(s)	255, 596, 634, 662, 706, 713, 731, 743, 959
HpyF10VI	GCNNNNNNNGC	4 cut(s)	179, 426, 602, 1057
HpyF3I	CTNAG	2 cut(s)	335, 949
Hsp92I	GRCGYC	1 cut(s)	603
Hsp92II	CATG	5 cut(s)	130, 349, 706, 910, 991
HspAI	GCGC	1 cut(s)	226
Ksp22I	TGATCA	1 cut(s)	903
Kzo9I	GATC	7 cut(s)	78, 218, 546, 573, 774, 903, 1020
LmnI	GCTCC	4 cut(s)	223, 467, 854, 1056
Lsp1109I	GCAGC	5 cut(s)	134, 438, 608, 725, 971
MabI	ACCWGGT	1 cut(s)	833
MaeI	CTAG	3 cut(s)	269, 528, 1037
MaeIII	GTNAC	3 cut(s)	478, 829, 970
MalI	GATC	7 cut(s)	80, 220, 548, 575, 776, 905, 1022
MbiI	CCGCTC	1 cut(s)	203
MboI	GATC	7 cut(s)	78, 218, 546, 573, 774, 903, 1020
MboII	GAAGA	5 cut(s)	182, 202, 253, 856, 1022
MfeI	CAATTG	1 cut(s)	663
MflI	RGATCY	1 cut(s)	573
MluCI	AATT	4 cut(s)	663, 726, 798, 966
MroXI	GAANNNNTTC	1 cut(s)	802
MseI	TTAA	4 cut(s)	351, 648, 725, 876
MslI	CAYNNNNRTG	1 cut(s)	344
MspA1I	CMGCKG	1 cut(s)	599
MspR9I	CCNGG	2 cut(s)	751, 835
MunI	CAATTG	1 cut(s)	663
MvaI	CCWGG	2 cut(s)	751, 835
MvnI	CGCG	2 cut(s)	83, 698
MwoI	GCNNNNNNNGC	4 cut(s)	179, 426, 602, 1057
NdeII	GATC	7 cut(s)	78, 218, 546, 573, 774, 903, 1020
NheI	GCTAGC	1 cut(s)	268
NlaIII	CATG	5 cut(s)	130, 349, 706, 910, 991
NlaIV	GGNNCC	3 cut(s)	139, 448, 575
NmuCI	GTSAC	1 cut(s)	829
NruI	TCGCGA	2 cut(s)	83, 698
PceI	AGGCCT	1 cut(s)	495
PdmI	GAANNNNTTC	1 cut(s)	802
PfeI	GAWTC	3 cut(s)	888, 929, 1031
PflMI	CCANNNNNTGG	2 cut(s)	68, 993
PkrI	GCNGC	5 cut(s)	124, 428, 598, 715, 961
Psp6I	CCWGG	2 cut(s)	749, 833
PspGI	CCWGG	2 cut(s)	749, 833
PspN4I	GGNNCC	3 cut(s)	139, 448, 575
PspPI	GGNCC	1 cut(s)	138
PstI	CTGCAG	2 cut(s)	636, 715
PsuI	RGATCY	1 cut(s)	573
PvuII	CAGCTG	1 cut(s)	599
RruI	TCGCGA	2 cut(s)	83, 698
RsaI	GTAC	3 cut(s)	286, 626, 656
RsaNI	GTAC	3 cut(s)	285, 625, 655
RseI	CAYNNNNRTG	1 cut(s)	344
SaqAI	TTAA	4 cut(s)	351, 648, 725, 876
SatI	GCNGC	5 cut(s)	123, 427, 597, 714, 960
Sau3AI	GATC	7 cut(s)	78, 218, 546, 573, 774, 903, 1020
Sau96I	GGNCC	1 cut(s)	138
ScrFI	CCNGG	2 cut(s)	751, 835
SexAI	ACCWGGT	1 cut(s)	833
SfcI	CTRYAG	2 cut(s)	632, 711
SinI	GGWCC	1 cut(s)	138
SmiMI	CAYNNNNRTG	1 cut(s)	344
SmlI	CTYRAG	1 cut(s)	421
SmoI	CTYRAG	1 cut(s)	421
Sse9I	AATT	4 cut(s)	663, 726, 798, 966
SseBI	AGGCCT	1 cut(s)	495
SsiI	CCGC	2 cut(s)	180, 201
SspI	AATATT	2 cut(s)	297, 307
SspMI	CTAG	3 cut(s)	269, 528, 1037
StuI	AGGCCT	1 cut(s)	495
StyD4I	CCNGG	2 cut(s)	749, 833
StyI	CCWWGG	1 cut(s)	577
TaaI	ACNGT	3 cut(s)	276, 811, 824
TaqI	TCGA	4 cut(s)	560, 806, 1023, 1029
TasI	AATT	4 cut(s)	663, 726, 798, 966
TatI	WGTACW	1 cut(s)	284
TfiI	GAWTC	3 cut(s)	888, 929, 1031
Tru1I	TTAA	4 cut(s)	351, 648, 725, 876
Tru9I	TTAA	4 cut(s)	351, 648, 725, 876
TscAI	CASTG	1 cut(s)	279
TseFI	GTSAC	1 cut(s)	829
TseI	GCWGC	5 cut(s)	122, 426, 596, 713, 959
Tsp45I	GTSAC	1 cut(s)	829
TspDTI	ATGAA	2 cut(s)	364, 661
TspRI	CASTG	1 cut(s)	279
Van91I	CCANNNNNTGG	2 cut(s)	68, 993
VpaK11BI	GGWCC	1 cut(s)	138
XmnI	GAANNNNTTC	1 cut(s)	802
XspI	CTAG	3 cut(s)	269, 528, 1037

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.