Rorug04G0351100

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001686
Physical Location & Seq
Forward (+)
52300122 .. 52300406
285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0351100.1

Sequence Viewer

Length: 285 bp
ATGTCACGTTGGTCTTCTGCTCCTTTGGGATGGTGCAAGATTAATGTGGATGGTTCCTATATTTATGCTACAAAATGTGGAGGAGTGGATTTTGTACTAAGGGATAGCTTGGGCAACTTTCTTGCTAGCGGAGGTATGCCTCTGCAGGGGTTATTATGCCTGGAGCACGCTGAGGTGTTGGACTGCAAGTTGGCAGCGAACTTTGCTGTGGAGCAGGGGTTTGTTCCTGCTTTACTAGAGCTTGATGCTCGATCGGGTGCTACAGAGGCAATTGGCGCAGCGTGA

Protein Analysis

94

Amino Acids

9.85

Weight (kDa)

4.64

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 15 - 84 2.7e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 129
AfaI GTAC 1 cut(s) 96
AfiI CCNNNNNNNGG 1 cut(s) 146
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 2 cut(s) 108, 241
AluI AGCT 2 cut(s) 108, 241
Alw21I GWGCWC 1 cut(s) 168
ApeKI GCWGC 2 cut(s) 194, 278
AseI ATTAAT 1 cut(s) 42
AspLEI GCGC 1 cut(s) 278
AsuNHI GCTAGC 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 6
Bbv12I GWGCWC 1 cut(s) 168
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 206
BccI CCATC 2 cut(s) 24, 44
BciT130I CCWGG 1 cut(s) 161
BfaI CTAG 2 cut(s) 126, 236
BfmI CTRYAG 2 cut(s) 143, 261
BisI GCNGC 2 cut(s) 195, 279
BlsI GCNGC 2 cut(s) 196, 280
Bme1390I CCNGG 1 cut(s) 161
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 235
BmtI GCTAGC 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 6
BpmI CTGGAG 1 cut(s) 182
Bpu10I CCTNAGC 1 cut(s) 171
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 146
BseBI CCWGG 1 cut(s) 161
BseGI GGATG 2 cut(s) 35, 55
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMII CTCAG 1 cut(s) 162
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 206
Bsh1285I CGRYCG 1 cut(s) 254
BsiEI CGRYCG 1 cut(s) 254
BsiHKAI GWGCWC 1 cut(s) 168
BslI CCNNNNNNNGG 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 1 cut(s) 251
BspACI CCGC 1 cut(s) 129
BspCNI CTCAG 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 147
BspOI GCTAGC 1 cut(s) 129
BssMI GATC 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 127, 168
BstDEI CTNAG 2 cut(s) 98, 171
BstF5I GGATG 2 cut(s) 35, 55
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 1 cut(s) 254
BstMBI GATC 1 cut(s) 251
BstMCI CGRYCG 1 cut(s) 254
BstMWI GCNNNNNNNGC 3 cut(s) 203, 266, 275
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 159
BstSFI CTRYAG 2 cut(s) 143, 261
BstV1I GCAGC 1 cut(s) 206
BstV2I GAAGAC 1 cut(s) 6
BtsCI GGATG 2 cut(s) 35, 55
Cac8I GCNNGC 2 cut(s) 127, 168
CfoI GCGC 1 cut(s) 278
Csp6I GTAC 1 cut(s) 95
CviJI RGCY 2 cut(s) 108, 241
CviKI_1 RGCY 2 cut(s) 108, 241
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 2 cut(s) 98, 171
DpnI GATC 1 cut(s) 253
DpnII GATC 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 159
FaiI YATR 4 cut(s) 60, 66, 137, 157
Fnu4HI GCNGC 2 cut(s) 195, 279
FokI GGATG 2 cut(s) 42, 62
Fsp4HI GCNGC 2 cut(s) 195, 279
FspBI CTAG 2 cut(s) 126, 236
GlaI GCGC 1 cut(s) 277
GluI GCNGC 2 cut(s) 195, 279
GsuI CTGGAG 1 cut(s) 182
HhaI GCGC 1 cut(s) 278
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 3 cut(s) 36, 145, 186
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 266, 275
HpyF3I CTNAG 2 cut(s) 98, 171
HpySE526I ACGT 1 cut(s) 7
HspAI GCGC 1 cut(s) 276
Kzo9I GATC 1 cut(s) 251
LmnI GCTCC 3 cut(s) 25, 163, 211
LpnPI CCDG 5 cut(s) 131, 146, 173, 200, 240
Lsp1109I GCAGC 1 cut(s) 206
LweI GCATC 1 cut(s) 235
MaeI CTAG 2 cut(s) 126, 236
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 1 cut(s) 3
MalI GATC 1 cut(s) 253
MboI GATC 1 cut(s) 251
MboII GAAGA 1 cut(s) 6
MfeI CAATTG 1 cut(s) 270
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 5 cut(s) 74, 125, 150, 166, 259
MseI TTAA 1 cut(s) 42
MspR9I CCNGG 1 cut(s) 161
MunI CAATTG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 3 cut(s) 203, 266, 275
NdeII GATC 1 cut(s) 251
NheI GCTAGC 1 cut(s) 125
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 3
PkrI GCNGC 2 cut(s) 196, 280
Ple19I CGATCG 1 cut(s) 254
PshBI ATTAAT 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 55
PstI CTGCAG 1 cut(s) 147
PvuI CGATCG 1 cut(s) 254
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
SaqAI TTAA 1 cut(s) 42
SatI GCNGC 2 cut(s) 195, 279
Sau3AI GATC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 161
SduI GDGCHC 1 cut(s) 168
SetI ASST 5 cut(s) 10, 110, 136, 177, 243
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 2 cut(s) 143, 261
Sse9I AATT 1 cut(s) 270
SsiI CCGC 1 cut(s) 129
SspMI CTAG 2 cut(s) 126, 236
StyD4I CCNGG 1 cut(s) 159
TaiI ACGT 1 cut(s) 10
TaqI TCGA 1 cut(s) 250
TasI AATT 1 cut(s) 270
TatI WGTACW 1 cut(s) 94
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 2 cut(s) 194, 278
Tsp45I GTSAC 1 cut(s) 3
VspI ATTAAT 1 cut(s) 42
XspI CTAG 2 cut(s) 126, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.