Rorug04G0351200

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001686
Physical Location & Seq
Forward (+)
52320950 .. 52323855
2906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0351200.1

Sequence Viewer

Length: 1371 bp
ATGACTGCAACTGCAAGAAATCAAAGAGAAGTGATCATGTGGCCTAGATTAGTGGCGAACAAAATTCTGAAGAAGCGATTGGGAAGCAACAACTTTGTAGCAGATTTCCCAAGCAACTCGGAGGACTCCACCTTGCAGCTGGAAACACCAAGTTTTGATCCAGAGAAACCGTTATCTTTATGCCCGACGACACCAGCTCCAAAAATCTTGAATCACCACAAGCACACACACAATTACAAGGTTTTTGTTAGTACATGGAATGTTGGTGGAGTTGAACCACGAGATGATATGAACATGGCAGATTGGATTACTGACATATCCTGTGACATCTATGTTTTTGGGTTTCAAGAAATTGTTCCTCTCAAAGCCTCAAATGTTCTGGGATCTGAGAATAGTATGATCTGCATGAAGTGGAACTCCTTCATTAGAGAAACTTTGAACAAGAAAATAAACCACCCGGGAGACAAGAATAAGGAAAAAAATGGAAAGTCAATCATTGTGGAAAGCAGTACCAATCCAGAATCACAGGATTACTTCCAGTGTATTATAAGTAAGCAAATGGTGGGAATACTAATAACAGTCTGGGTTCGAAGAAATCTTCGTCCTTTGATTCGGAATCCGAGTGTTTCATGTGTTGGCTGTGGCATCATGGGTTGCCTCGGCAATAAGGGTTCGGTGTCAGTTAGGTTTCAACTGCATGAAACAAGCTTCTGCTTCGTGTGCTGTCATCTAGCTTCAGGGGGAAGACAAGGAGATGAGAAACTCAGAAACTCGAACGTAGCTGAAATCTTGAACAGAACAAGTTTCCCTAGAGGTCCATTGCTTGATTTGCCACGAAAGATCCTAGACCACCAACGAGTAATCTTCTTGGGAGATTTGAATTACAGAATTTCCCTTCCAGAAGCAACCACACGGTCATTAGTGGATAGCAGAGATTGGAATGCCTTACTGAAGAGCGATCAGCTGAGTATGGAGCTCAATGATGGTCAAGTATTTGAAGGTTGGCTTGAAGGAAGTATCAAATTTGCCCCGACTTACAAATATTTTCCAGATTCTGATACATATTATGGATGTCCTCCTCCTGCAAGGAAAGGTGAAAAGAGGCGTGCTCCTGCATGGTGTGATCGGATAATTTGGTATGGAAAAGGGTTGAAGCAACATGTATACGACCGAGGTGAATCAAAATTGTCTGACCACAGGCCAGTGAAGTCAATATTTACTGCCCAAGTTGAAGTTCTACCATCGCTTATGAGAGGATACCAAAGCTTTTTTCTTTCAGACAGGTTTGACAAAATTTCAAGCCAGTTTGAGGTACCCTCCACCGACGATCGATTTCTATGTCAACGCAGATCATCAAGCTTCAAAGCTTGA

Protein Analysis

456

Amino Acids

51.9

Weight (kDa)

9.05

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos2 PF22669 85 - 404 5.1e-69 Endonuclease/Exonuclease/phosphatase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 548
Acc65I GGTACC 1 cut(s) 1312
AccB1I GGYRCC 1 cut(s) 1312
AccI GTMKAC 1 cut(s) 1164
AclWI GGATC 3 cut(s) 152, 391, 835
AcsI RAATTY 4 cut(s) 63, 888, 1022, 1293
AcuI CTGAAG 3 cut(s) 89, 720, 971
AfaI GTAC 3 cut(s) 253, 511, 1314
AfiI CCNNNNNNNGG 1 cut(s) 1309
AflIII ACRYGT 1 cut(s) 1159
AjuI GAANNNNNNNTTGG 6 cut(s) 62, 94, 1218, 1250, 1254, 1286
Alw21I GWGCWC 2 cut(s) 978, 1111
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 3 cut(s) 152, 391, 835
AlwNI CAGNNNCTG 1 cut(s) 1055
Ama87I CYCGRG 1 cut(s) 457
AoxI GGCC 2 cut(s) 41, 1199
ApeKI GCWGC 1 cut(s) 136
ApoI RAATTY 4 cut(s) 63, 888, 1022, 1293
Asp700I GAANNNNTTC 2 cut(s) 354, 419
Asp718I GGTACC 1 cut(s) 1312
AspS9I GGNCC 1 cut(s) 815
AsuC2I CCSGG 2 cut(s) 458, 459
AsuHPI GGTGA 3 cut(s) 206, 1106, 1187
AsuII TTCGAA 1 cut(s) 589
AvaI CYCGRG 1 cut(s) 457
AvaII GGWCC 1 cut(s) 815
BanI GGYRCC 1 cut(s) 1312
BanII GRGCYC 1 cut(s) 978
BauI CACGAG 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 751
Bbv12I GWGCWC 2 cut(s) 978, 1111
BbvI GCAGC 1 cut(s) 148
BccI CCATC 2 cut(s) 977, 1249
BciVI GTATCC 1 cut(s) 1250
BclI TGATCA 1 cut(s) 33
BcnI CCSGG 2 cut(s) 458, 459
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 4 cut(s) 45, 731, 810, 845
BfuI GTATCC 1 cut(s) 1250
BisI GCNGC 1 cut(s) 137
BlsI GCNGC 1 cut(s) 138
Bme1390I CCNGG 2 cut(s) 458, 459
Bme18I GGWCC 1 cut(s) 815
BmeT110I CYCGRG 1 cut(s) 457
BmgT120I GGNCC 1 cut(s) 815
BmiI GGNNCC 1 cut(s) 1314
BmrFI CCNGG 2 cut(s) 458, 459
BmsI GCATC 1 cut(s) 654
BpiI GAAGAC 1 cut(s) 751
BplI GAGNNNNNCTC 4 cut(s) 1093, 1125, 1301, 1333
Bpu14I TTCGAA 1 cut(s) 589
BpuMI CCSGG 2 cut(s) 458, 459
Bsa29I ATCGAT 1 cut(s) 1330
BsaJI CCNNGG 3 cut(s) 457, 658, 1171
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 1 cut(s) 1309
Bse1I ACTGG 3 cut(s) 538, 1202, 1303
Bse3DI GCAATG 1 cut(s) 818
BseCI ATCGAT 1 cut(s) 1330
BseDI CCNNGG 3 cut(s) 457, 658, 1171
BseGI GGATG 1 cut(s) 1076
BseLI CCNNNNNNNGG 1 cut(s) 1309
BseMI GCAATG 1 cut(s) 818
BseMII CTCAG 3 cut(s) 378, 778, 956
BseNI ACTGG 3 cut(s) 538, 1202, 1303
BseRI GAGGAG 1 cut(s) 1068
BseXI GCAGC 1 cut(s) 148
Bsh1285I CGRYCG 2 cut(s) 1171, 1330
BshFI GGCC 2 cut(s) 43, 1201
BshNI GGYRCC 1 cut(s) 1312
BshVI ATCGAT 1 cut(s) 1330
BsiEI CGRYCG 2 cut(s) 1171, 1330
BsiHKAI GWGCWC 2 cut(s) 978, 1111
BsiHKCI CYCGRG 1 cut(s) 457
BsiSI CCGG 1 cut(s) 458
BslI CCNNNNNNNGG 1 cut(s) 1309
BsmAI GTCTC 1 cut(s) 456
BsmI GAATGC 1 cut(s) 946
BsnI GGCC 2 cut(s) 43, 1201
BsoBI CYCGRG 1 cut(s) 457
Bsp119I TTCGAA 1 cut(s) 589
Bsp1286I GDGCHC 2 cut(s) 978, 1111
Bsp143I GATC 9 cut(s) 33, 157, 383, 399, 840, 958, 1123, 1327, 1349
BspANI GGCC 2 cut(s) 43, 1201
BspCNI CTCAG 3 cut(s) 379, 777, 957
BspDI ATCGAT 1 cut(s) 1330
BspLI GGNNCC 1 cut(s) 1314
BspPI GGATC 3 cut(s) 152, 391, 835
BspQI GCTCTTC 1 cut(s) 947
BspT104I TTCGAA 1 cut(s) 589
BspT107I GGYRCC 1 cut(s) 1312
BsrDI GCAATG 1 cut(s) 818
BsrI ACTGG 3 cut(s) 538, 1202, 1303
BssECI CCNNGG 3 cut(s) 457, 658, 1171
BssMI GATC 9 cut(s) 33, 157, 383, 399, 840, 958, 1123, 1327, 1349
BssNAI GTATAC 1 cut(s) 1165
BssSI CACGAG 1 cut(s) 279
Bst1107I GTATAC 1 cut(s) 1165
Bst2BI CACGAG 1 cut(s) 279
Bst4CI ACNGT 3 cut(s) 171, 580, 915
Bst6I CTCTTC 1 cut(s) 947
BstBI TTCGAA 1 cut(s) 589
BstC8I GCNNGC 1 cut(s) 1107
BstDEI CTNAG 3 cut(s) 387, 764, 965
BstF5I GGATG 1 cut(s) 1076
BstKTI GATC 9 cut(s) 36, 160, 386, 402, 843, 961, 1126, 1330, 1352
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 9 cut(s) 33, 157, 383, 399, 840, 958, 1123, 1327, 1349
BstMCI CGRYCG 2 cut(s) 1171, 1330
BstMWI GCNNNNNNNGC 2 cut(s) 720, 829
BstNSI RCATGY 1 cut(s) 1163
BstSCI CCNGG 2 cut(s) 456, 457
BstV1I GCAGC 1 cut(s) 148
BstV2I GAAGAC 1 cut(s) 751
BstX2I RGATCY 2 cut(s) 383, 840
BstYI RGATCY 2 cut(s) 383, 840
BstZ17I GTATAC 1 cut(s) 1165
Bsu15I ATCGAT 1 cut(s) 1330
BsuI GTATCC 1 cut(s) 1250
BsuRI GGCC 2 cut(s) 43, 1201
BsuTUI ATCGAT 1 cut(s) 1330
BtgZI GCGATG 1 cut(s) 1227
BtsCI GGATG 1 cut(s) 1076
BtsIMutI CAGTG 2 cut(s) 545, 1209
Cac8I GCNNGC 1 cut(s) 1107
CaiI CAGNNNCTG 1 cut(s) 1055
Cfr13I GGNCC 1 cut(s) 815
Cfr9I CCCGGG 1 cut(s) 457
ClaI ATCGAT 1 cut(s) 1330
Csp6I GTAC 3 cut(s) 252, 510, 1313
CspCI CAANNNNNGTGG 2 cut(s) 480, 515
CviAII CATG 9 cut(s) 37, 255, 295, 406, 630, 649, 698, 1116, 1160
CviQI GTAC 3 cut(s) 252, 510, 1313
DdeI CTNAG 3 cut(s) 387, 764, 965
DpnI GATC 9 cut(s) 35, 159, 385, 401, 842, 960, 1125, 1329, 1351
DpnII GATC 9 cut(s) 33, 157, 383, 399, 840, 958, 1123, 1327, 1349
Eam1104I CTCTTC 1 cut(s) 947
EarI CTCTTC 1 cut(s) 947
Ecl136II GAGCTC 1 cut(s) 976
Eco24I GRGCYC 1 cut(s) 978
Eco47I GGWCC 1 cut(s) 815
Eco53kI GAGCTC 1 cut(s) 976
Eco57I CTGAAG 3 cut(s) 89, 720, 971
Eco88I CYCGRG 1 cut(s) 457
EcoICRI GAGCTC 1 cut(s) 976
EcoT38I GRGCYC 1 cut(s) 978
FaeI CATG 9 cut(s) 40, 258, 298, 409, 633, 652, 701, 1119, 1163
FalI AAGNNNNNCTT 2 cut(s) 990, 1022
FatI CATG 9 cut(s) 36, 254, 294, 405, 629, 648, 697, 1115, 1159
FbaI TGATCA 1 cut(s) 33
FblI GTMKAC 1 cut(s) 1164
Fnu4HI GCNGC 1 cut(s) 137
FokI GGATG 1 cut(s) 1083
FriOI GRGCYC 1 cut(s) 978
Fsp4HI GCNGC 1 cut(s) 137
FspBI CTAG 4 cut(s) 45, 731, 810, 845
GluI GCNGC 1 cut(s) 137
HaeIII GGCC 2 cut(s) 43, 1201
HapII CCGG 1 cut(s) 458
Hin1II CATG 9 cut(s) 40, 258, 298, 409, 633, 652, 701, 1119, 1163
HincII GTYRAC 1 cut(s) 1343
HindII GTYRAC 1 cut(s) 1343
HindIII AAGCTT 4 cut(s) 706, 1264, 1357, 1365
HinfI GANTC 7 cut(s) 125, 211, 521, 610, 616, 1052, 1178
HpaII CCGG 1 cut(s) 458
HphI GGTGA 3 cut(s) 206, 1106, 1187
Hpy166II GTNNAC 2 cut(s) 1165, 1343
Hpy188III TCNNGA 7 cut(s) 161, 208, 347, 518, 790, 899, 1049
Hpy8I GTNNAC 2 cut(s) 1165, 1343
Hpy99I CGWCG 2 cut(s) 190, 1328
HpyAV CCTTC 4 cut(s) 430, 905, 992, 1004
HpyCH4III ACNGT 3 cut(s) 171, 580, 915
HpyCH4IV ACGT 1 cut(s) 777
HpyCH4V TGCA 7 cut(s) 8, 14, 136, 405, 697, 1085, 1115
HpyF10VI GCNNNNNNNGC 2 cut(s) 720, 829
HpyF3I CTNAG 3 cut(s) 387, 764, 965
HpySE526I ACGT 1 cut(s) 777
Hsp92II CATG 9 cut(s) 40, 258, 298, 409, 633, 652, 701, 1119, 1163
KpnI GGTACC 1 cut(s) 1316
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 9 cut(s) 33, 157, 383, 399, 840, 958, 1123, 1327, 1349
LguI GCTCTTC 1 cut(s) 947
LmnI GCTCC 3 cut(s) 202, 973, 1114
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 1 cut(s) 654
MaeI CTAG 4 cut(s) 45, 731, 810, 845
MaeII ACGT 1 cut(s) 777
MaeIII GTNAC 1 cut(s) 323
MalI GATC 9 cut(s) 35, 159, 385, 401, 842, 960, 1125, 1329, 1351
MboI GATC 9 cut(s) 33, 157, 383, 399, 840, 958, 1123, 1327, 1349
MboII GAAGA 6 cut(s) 82, 590, 603, 756, 856, 964
MflI RGATCY 2 cut(s) 383, 840
MhlI GDGCHC 2 cut(s) 978, 1111
MluCI AATT 9 cut(s) 63, 232, 351, 880, 888, 1022, 1131, 1184, 1293
MlyI GAGTC 1 cut(s) 119
MroXI GAANNNNTTC 2 cut(s) 354, 419
MspA1I CMGCKG 2 cut(s) 139, 964
MspI CCGG 1 cut(s) 458
MspR9I CCNGG 2 cut(s) 458, 459
Mva1269I GAATGC 1 cut(s) 946
MwoI GCNNNNNNNGC 2 cut(s) 720, 829
NciI CCSGG 2 cut(s) 458, 459
NdeII GATC 9 cut(s) 33, 157, 383, 399, 840, 958, 1123, 1327, 1349
NlaIII CATG 9 cut(s) 40, 258, 298, 409, 633, 652, 701, 1119, 1163
NlaIV GGNNCC 1 cut(s) 1314
NmeAIII GCCGAG 1 cut(s) 639
NmuCI GTSAC 1 cut(s) 323
NspI RCATGY 1 cut(s) 1163
NspV TTCGAA 1 cut(s) 589
PciI ACATGT 1 cut(s) 1159
PciSI GCTCTTC 1 cut(s) 947
PctI GAATGC 1 cut(s) 946
PdmI GAANNNNTTC 2 cut(s) 354, 419
PfeI GAWTC 6 cut(s) 211, 521, 610, 616, 1052, 1178
PkrI GCNGC 1 cut(s) 138
Ple19I CGATCG 1 cut(s) 1330
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PscI ACATGT 1 cut(s) 1159
PsiI TTATAA 1 cut(s) 548
Psp124BI GAGCTC 1 cut(s) 978
PspN4I GGNNCC 1 cut(s) 1314
PspPI GGNCC 1 cut(s) 815
PstNI CAGNNNCTG 1 cut(s) 1055
PsuI RGATCY 2 cut(s) 383, 840
PvuI CGATCG 1 cut(s) 1330
PvuII CAGCTG 2 cut(s) 139, 964
RsaI GTAC 3 cut(s) 253, 511, 1314
RsaNI GTAC 3 cut(s) 252, 510, 1313
SacI GAGCTC 1 cut(s) 978
SapI GCTCTTC 1 cut(s) 947
SatI GCNGC 1 cut(s) 137
Sau3AI GATC 9 cut(s) 33, 157, 383, 399, 840, 958, 1123, 1327, 1349
Sau96I GGNCC 1 cut(s) 815
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 458, 459
SduI GDGCHC 2 cut(s) 978, 1111
SfaNI GCATC 1 cut(s) 654
SfuI TTCGAA 1 cut(s) 589
SinI GGWCC 1 cut(s) 815
SmaI CCCGGG 1 cut(s) 459
Sse9I AATT 9 cut(s) 63, 232, 351, 880, 888, 1022, 1131, 1184, 1293
SspI AATATT 2 cut(s) 1043, 1215
SspMI CTAG 4 cut(s) 45, 731, 810, 845
SstI GAGCTC 1 cut(s) 978
StyD4I CCNGG 2 cut(s) 456, 457
TaaI ACNGT 3 cut(s) 171, 580, 915
TaiI ACGT 1 cut(s) 780
TaqI TCGA 3 cut(s) 589, 773, 1330
TaqII GACCGA 1 cut(s) 1185
TasI AATT 9 cut(s) 63, 232, 351, 880, 888, 1022, 1131, 1184, 1293
TatI WGTACW 1 cut(s) 251
TfiI GAWTC 6 cut(s) 211, 521, 610, 616, 1052, 1178
TscAI CASTG 2 cut(s) 545, 1209
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 5 cut(s) 305, 412, 422, 618, 714
TspMI CCCGGG 1 cut(s) 457
TspRI CASTG 2 cut(s) 545, 1209
VpaK11BI GGWCC 1 cut(s) 815
XapI RAATTY 4 cut(s) 63, 888, 1022, 1293
XceI RCATGY 1 cut(s) 1163
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XmaI CCCGGG 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 1164
XmnI GAANNNNTTC 2 cut(s) 354, 419
XspI CTAG 4 cut(s) 45, 731, 810, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.