Rroxscaffold_2G00135210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0020729
Physical Location & Seq
Forward (+)
72812181 .. 72815761
3581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00135210.1

Sequence Viewer

Length: 411 bp
ATGAAGAGAATCGCAGCTAGTGATGAAGTATATGAAGATTACTTCAAGGTCTCTTCCGACGAGGCCCTATCCAATTCCGAGGAGGAGAAGCCGATCGAAGACGAAGAGGACGAGGAAGAACTCGAGGCCGTCGCTTACTCCGCCGACTCCGAGGACAAGACTATTGGGGGCCGGTACGAATCAAAAGTATTTTTTCATCTTAACATAATCGAGCACCACAGCCTTCTTCTCCATCTCTCTCTTCCACACACGGACACCTCCCACCTCTACATCTCCTCCTCCCGAATCGGTTGGAAGAGTTCGGGAAAATGGCCGCATGGACTTGGACCTTGCCCAAGGTTCAGCTACGATCTGCAAGGAAATTGGCAAAAAACGGGCTGCTTATTGGTAATAAATGGGATGAGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

15.49

Weight (kDa)

4.88

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020729)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001167.1_g000025
rosa_roxburghii Rroxscaffold_2G00135210 Rroxscaffold_3G00238660
rosa_samantha Rh2CG175700 Rh4AG273400 Rh4BG279000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 141, 314
AcoI YGGCCR 1 cut(s) 311
AfaI GTAC 1 cut(s) 176
AgsI TTSAA 1 cut(s) 46
AluBI AGCT 2 cut(s) 17, 345
AluI AGCT 2 cut(s) 17, 345
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 55
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 4 cut(s) 63, 126, 169, 311
ApeKI GCWGC 2 cut(s) 14, 378
AspS9I GGNCC 3 cut(s) 64, 169, 326
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 1 cut(s) 326
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 2 cut(s) 26, 365
BccI CCATC 1 cut(s) 240
BceAI ACGGC 1 cut(s) 113
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 18
BisI GCNGC 3 cut(s) 15, 314, 379
BlsI GCNGC 3 cut(s) 16, 315, 380
Bme18I GGWCC 1 cut(s) 326
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 3 cut(s) 64, 169, 326
BmiI GGNNCC 1 cut(s) 170
BpiI GAAGAC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 3 cut(s) 78, 150, 335
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bse118I RCCGGY 1 cut(s) 171
BseDI CCNNGG 3 cut(s) 78, 150, 335
BseGI GGATG 1 cut(s) 405
BseRI GAGGAG 4 cut(s) 95, 98, 265, 268
BseXI GCAGC 2 cut(s) 26, 365
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 4 cut(s) 65, 128, 171, 313
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 1 cut(s) 172
BsmAI GTCTC 1 cut(s) 55
BsnI GGCC 4 cut(s) 65, 128, 171, 313
Bso31I GGTCTC 1 cut(s) 55
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 93, 349
BspACI CCGC 2 cut(s) 141, 314
BspANI GGCC 4 cut(s) 65, 128, 171, 313
BspLI GGNNCC 1 cut(s) 170
BspTNI GGTCTC 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 171
BssAI RCCGGY 1 cut(s) 171
BssECI CCNNGG 3 cut(s) 78, 150, 335
BssMI GATC 2 cut(s) 93, 349
BssT1I CCWWGG 1 cut(s) 335
Bst6I CTCTTC 4 cut(s) 58, 99, 246, 290
BstF5I GGATG 1 cut(s) 405
BstKTI GATC 2 cut(s) 96, 352
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 2 cut(s) 93, 349
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstV1I GCAGC 2 cut(s) 26, 365
BstV2I GAAGAC 1 cut(s) 105
BsuRI GGCC 4 cut(s) 65, 128, 171, 313
BtsCI GGATG 1 cut(s) 405
Cfr10I RCCGGY 1 cut(s) 171
Cfr13I GGNCC 3 cut(s) 64, 169, 326
Csp6I GTAC 1 cut(s) 175
CviAII CATG 1 cut(s) 317
CviJI RGCY 9 cut(s) 17, 65, 91, 128, 171, 222, 313, 345, 378
CviKI_1 RGCY 9 cut(s) 17, 65, 91, 128, 171, 222, 313, 345, 378
CviQI GTAC 1 cut(s) 175
DpnI GATC 2 cut(s) 95, 351
DpnII GATC 2 cut(s) 93, 349
EaeI YGGCCR 1 cut(s) 311
Eam1104I CTCTTC 4 cut(s) 58, 99, 246, 290
EarI CTCTTC 4 cut(s) 58, 99, 246, 290
EciI GGCGGA 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 335
Eco31I GGTCTC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 335
FaeI CATG 1 cut(s) 320
FaiI YATR 4 cut(s) 31, 33, 206, 318
FatI CATG 1 cut(s) 316
Fnu4HI GCNGC 3 cut(s) 15, 314, 379
Fsp4HI GCNGC 3 cut(s) 15, 314, 379
FspBI CTAG 1 cut(s) 18
GluI GCNGC 3 cut(s) 15, 314, 379
HaeIII GGCC 4 cut(s) 65, 128, 171, 313
HapII CCGG 1 cut(s) 172
Hin1II CATG 1 cut(s) 320
HinfI GANTC 4 cut(s) 9, 146, 179, 285
HpaII CCGG 1 cut(s) 172
Hpy188I TCNGA 3 cut(s) 58, 79, 151
Hpy188III TCNNGA 2 cut(s) 282, 303
Hpy99I CGWCG 2 cut(s) 62, 134
HpyAV CCTTC 1 cut(s) 233
HpyCH4V TGCA 1 cut(s) 355
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
Hsp92II CATG 1 cut(s) 320
Kzo9I GATC 2 cut(s) 93, 349
LpnPI CCDG 1 cut(s) 185
Lsp1109I GCAGC 2 cut(s) 26, 365
MaeI CTAG 1 cut(s) 18
MalI GATC 2 cut(s) 95, 351
MboI GATC 2 cut(s) 93, 349
MboII GAAGA 9 cut(s) 16, 45, 47, 110, 116, 128, 218, 233, 307
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 2 cut(s) 73, 361
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 2 cut(s) 81, 272
MseI TTAA 1 cut(s) 201
MspI CCGG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 2 cut(s) 93, 349
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 1 cut(s) 170
PaeR7I CTCGAG 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PfeI GAWTC 3 cut(s) 9, 179, 285
PkrI GCNGC 3 cut(s) 16, 315, 380
Ple19I CGATCG 1 cut(s) 96
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PspN4I GGNNCC 1 cut(s) 170
PspPI GGNCC 3 cut(s) 64, 169, 326
PspXI VCTCGAGB 1 cut(s) 122
PvuI CGATCG 1 cut(s) 96
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 3 cut(s) 15, 314, 379
Sau3AI GATC 2 cut(s) 93, 349
Sau96I GGNCC 3 cut(s) 64, 169, 326
SchI GAGTC 1 cut(s) 140
SduI GDGCHC 1 cut(s) 216
SetI ASST 7 cut(s) 19, 51, 260, 267, 331, 341, 347
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 326
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 2 cut(s) 73, 361
SsiI CCGC 2 cut(s) 141, 314
SspMI CTAG 1 cut(s) 18
StyI CCWWGG 1 cut(s) 335
TaqI TCGA 3 cut(s) 96, 123, 210
TasI AATT 2 cut(s) 73, 361
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 3 cut(s) 9, 179, 285
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TseI GCWGC 2 cut(s) 14, 378
TspDTI ATGAA 4 cut(s) 17, 39, 48, 185
TspGWI ACGGA 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 326
XhoI CTCGAG 1 cut(s) 122
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.