Rroxscaffold_3G00238660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0020729
Physical Location & Seq
Reverse (-)
26892479 .. 26895428
2950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00238660.1

Sequence Viewer

Length: 381 bp
ATGAAGCCCCAGGTCTCTTTCGACGAAGCTCTATCCAATTCCAAGGAGGAGGAACCGATCGACGACGAAGAGCTCGAGGCCGTCGCTCGCTCCGCCGACTCCGAGGACAAGACTAGTGGGGGTCGATTAATAGCAATGGCAAAGAGGAAGGTTGCTGCAATAGACAAAGACGATGTACCGGTTGGAACAAAGAAGATGAGCACGAGAGATCGGATTACATACGGTATAAATGTAATCTGCATGTATTCGGGTTTAGCAGCGATTGGAAAAGAGGCTTGGAGGAAGCTAAAGAAGACGCCATTTTGGATCAGATTTTATGATGAGGAGATCAAGAAGGATCAGATTTGCAAATCGGATGACCTCACAAAACGTTTGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

14.29

Weight (kDa)

6.36

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020729)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001167.1_g000025
rosa_roxburghii Rroxscaffold_2G00135210 Rroxscaffold_3G00238660
rosa_samantha Rh2CG175700 Rh4AG273400 Rh4BG279000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 93
AclI AACGTT 1 cut(s) 370
AclWI GGATC 2 cut(s) 314, 345
AcyI GRCGYC 1 cut(s) 296
AfaI GTAC 1 cut(s) 177
AgeI ACCGGT 1 cut(s) 178
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 1 cut(s) 9
AjuI GAANNNNNNNTTGG 2 cut(s) 259, 291
AluBI AGCT 3 cut(s) 29, 73, 286
AluI AGCT 3 cut(s) 29, 73, 286
Alw21I GWGCWC 2 cut(s) 75, 203
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 2 cut(s) 314, 345
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 2 cut(s) 155, 257
AseI ATTAAT 1 cut(s) 128
AsiGI ACCGGT 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 74
BanII GRGCYC 1 cut(s) 75
BauI CACGAG 1 cut(s) 202
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 2 cut(s) 75, 203
BbvI GCAGC 2 cut(s) 142, 269
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 1 cut(s) 11
BcoDI GTCTC 1 cut(s) 19
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 377
BisI GCNGC 2 cut(s) 156, 258
BlsI GCNGC 2 cut(s) 157, 259
Bme1390I CCNGG 1 cut(s) 11
BmeT110I CYCGRG 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 11
BpiI GAAGAC 1 cut(s) 299
BsaHI GRCGYC 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 3 cut(s) 9, 42, 102
BsaWI WCCGGW 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 178
Bse3DI GCAATG 1 cut(s) 141
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 3 cut(s) 9, 42, 102
BseGI GGATG 1 cut(s) 361
BseMI GCAATG 1 cut(s) 141
BseRI GAGGAG 2 cut(s) 62, 338
BseXI GCAGC 2 cut(s) 142, 269
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 1 cut(s) 80
BshTI ACCGGT 1 cut(s) 178
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 2 cut(s) 75, 203
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 19
BsnI GGCC 1 cut(s) 80
Bso31I GGTCTC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 75, 203
Bsp143I GATC 5 cut(s) 57, 208, 306, 327, 337
BspACI CCGC 1 cut(s) 93
BspANI GGCC 1 cut(s) 80
BspLI GGNNCC 1 cut(s) 54
BspPI GGATC 2 cut(s) 314, 345
BspQI GCTCTTC 1 cut(s) 63
BspTNI GGTCTC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 3 cut(s) 9, 42, 102
BssMI GATC 5 cut(s) 57, 208, 306, 327, 337
BssNI GRCGYC 1 cut(s) 296
BssSI CACGAG 1 cut(s) 202
BssT1I CCWWGG 1 cut(s) 42
Bst2BI CACGAG 1 cut(s) 202
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 63
BstACI GRCGYC 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 88
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 5 cut(s) 60, 211, 309, 330, 340
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 5 cut(s) 57, 208, 306, 327, 337
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 377
BstV1I GCAGC 2 cut(s) 142, 269
BstV2I GAAGAC 1 cut(s) 299
BsuRI GGCC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 361
Cac8I GCNNGC 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 178
CseI GACGC 1 cut(s) 304
Csp6I GTAC 1 cut(s) 176
CspAI ACCGGT 1 cut(s) 178
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviAII CATG 1 cut(s) 241
CviJI RGCY 6 cut(s) 7, 29, 73, 80, 275, 286
CviKI_1 RGCY 6 cut(s) 7, 29, 73, 80, 275, 286
CviQI GTAC 1 cut(s) 176
DpnI GATC 5 cut(s) 59, 210, 308, 329, 339
DpnII GATC 5 cut(s) 57, 208, 306, 327, 337
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
EciI GGCGGA 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 42
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 244
FaiI YATR 4 cut(s) 220, 227, 242, 318
FatI CATG 1 cut(s) 240
Fnu4HI GCNGC 2 cut(s) 156, 258
FokI GGATG 1 cut(s) 368
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 156, 258
FspBI CTAG 1 cut(s) 114
GluI GCNGC 2 cut(s) 156, 258
HaeIII GGCC 1 cut(s) 80
HapII CCGG 1 cut(s) 179
HgaI GACGC 1 cut(s) 304
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 1 cut(s) 244
HinfI GANTC 1 cut(s) 98
HpaII CCGG 1 cut(s) 179
Hpy188I TCNGA 5 cut(s) 103, 213, 311, 342, 355
Hpy188III TCNNGA 1 cut(s) 331
Hpy99I CGWCG 4 cut(s) 26, 65, 68, 86
HpyAV CCTTC 2 cut(s) 142, 328
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 1 cut(s) 370
HpyCH4V TGCA 3 cut(s) 158, 240, 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpySE526I ACGT 1 cut(s) 370
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 1 cut(s) 244
Kzo9I GATC 5 cut(s) 57, 208, 306, 327, 337
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 2 cut(s) 23, 192
Lsp1109I GCAGC 2 cut(s) 142, 269
MaeI CTAG 1 cut(s) 114
MaeII ACGT 1 cut(s) 370
MalI GATC 5 cut(s) 59, 210, 308, 329, 339
MboI GATC 5 cut(s) 57, 208, 306, 327, 337
MboII GAAGA 3 cut(s) 80, 205, 304
MhlI GDGCHC 2 cut(s) 75, 203
MluCI AATT 1 cut(s) 37
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 9 cut(s) 40, 43, 70, 97, 138, 265, 273, 316, 371
MseI TTAA 1 cut(s) 128
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 92
NdeII GATC 5 cut(s) 57, 208, 306, 327, 337
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 1 cut(s) 54
NspI RCATGY 1 cut(s) 244
PaeR7I CTCGAG 1 cut(s) 74
PciSI GCTCTTC 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PinAI ACCGGT 1 cut(s) 178
PkrI GCNGC 2 cut(s) 157, 259
Ple19I CGATCG 1 cut(s) 60
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PshBI ATTAAT 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 75
Psp1406I AACGTT 1 cut(s) 370
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 54
PspXI VCTCGAGB 1 cut(s) 74
PvuI CGATCG 1 cut(s) 60
RsaI GTAC 1 cut(s) 177
RsaNI GTAC 1 cut(s) 176
SacI GAGCTC 1 cut(s) 75
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 2 cut(s) 156, 258
Sau3AI GATC 5 cut(s) 57, 208, 306, 327, 337
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 2 cut(s) 75, 203
SetI ASST 7 cut(s) 15, 31, 75, 153, 288, 363, 373
SfcI CTRYAG 1 cut(s) 377
Sfr274I CTCGAG 1 cut(s) 74
SlaI CTCGAG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 1 cut(s) 37
SsiI CCGC 1 cut(s) 93
SspMI CTAG 1 cut(s) 114
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 1 cut(s) 373
TaqI TCGA 4 cut(s) 21, 60, 75, 124
TasI AATT 1 cut(s) 37
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TseI GCWGC 2 cut(s) 155, 257
TspDTI ATGAA 1 cut(s) 17
VspI ATTAAT 1 cut(s) 128
XceI RCATGY 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 74
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.