Rroxscaffold_2G00141210

Pyridine nucleotide-disulfide oxidoreductase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011262
Physical Location & Seq
Reverse (-)
79496807 .. 79501148
4342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141210.1

Sequence Viewer

Length: 924 bp
ATGTGGCGGAGGAGCTTTAGCAGCGGCGCAGCCGCAAGCGCGTTGAAGGACAAGAAATGGGACGCGCTGGTCATCGGAGGCGGCCACAACGGCCTCACTGCAGCCGCGTACCTCGCACGCGCCGGCATATCCGTCGCAGTCCTCGAACGCCGCCACGTCATCGGCGGAGCCGCCGTGACGGAAGAGCTCATTCCAGGATTCAAGTTCTCCCGGTGTAGCTACCTCCAGAGCCTCCTCCGTCCCGCCGTTATCAAGGAATTAGAGCTAGCTCGGCATGGAATGAAGTTACTGAAGAGGAGTCCGTCGTCGTTTACGCCTTGTTTGGACGGACGTTATCTTCTTTTAGGGCCGGAAAAGGACCTTAACCATTCAGAGATTGCTAAATTCTCCAAGCTAGACGCTGATGCTTATCCAAGATATGAGAATCAGCTAGAGAAGTTCTGTCAGTTTATGGATCCTTTGCTGGATTCAGCTCCTCCAGAATCTTCGCAAGGCGCCTCCTCGGCTACTTTTGGTGACCGGTTGAAGGACAAGATGCACAAGTCGATGTTTTGGGCTCAGCTTCTGAGACAGGCTGCTGTCTTAGGCCAGAAAGATATGGTAGACTTTATGGACCTTCTGTTATCCCCGGCTTCGAAGGTTTTGAACAACTGGTTTGAGACTGATGTTCTGAAGGCAACTCTTGCAACAGATGCTGTAATAGGAACTACGGGAAGTGTCCATACACCAGGGAGTGGATATGTTCTGCTACATCATGTGATGGGAGAAACTGATGGCGAGCGTGGAATTTGGTCGTATGTTGAAGGTGGGATGGGTTCAGTATCCTTGGCTATTGGTAATGCTGCTAGGGAAGCTGGGGCTCATATTGTCACAAGTGCAGAGGTATTTATGCCTAGAAATGAGCTAGGGTTCAGCAAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase DAO NAD_binding_8

Protein Analysis

307

Amino Acids

33.32

Weight (kDa)

7.72

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 18 - 58 3.5e-06 Thi4 family
DAO PF01266 21 - 57 1.5e-07 FAD dependent oxidoreductase
FAD_oxidored PF12831 21 - 62 2.2e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 24 - 71 2.6e-11 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 31 - 293 1e-06 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 68
AccB1I GGYRCC 1 cut(s) 494
AccB7I CCANNNNNTGG 1 cut(s) 734
AccI GTMKAC 1 cut(s) 603
AccII CGCG 4 cut(s) 41, 65, 107, 120
AciI CCGC 9 cut(s) 7, 24, 33, 81, 105, 151, 165, 171, 243
AclWI GGATC 2 cut(s) 449, 462
AcoI YGGCCR 1 cut(s) 82
AcsI RAATTY 2 cut(s) 383, 786
AcuI CTGAAG 2 cut(s) 311, 692
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 2 cut(s) 526, 734
AgeI ACCGGT 1 cut(s) 519
AgsI TTSAA 5 cut(s) 46, 202, 526, 646, 803
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 2 cut(s) 193, 727
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 2 cut(s) 562, 653
AlwI GGATC 2 cut(s) 449, 462
AlwNI CAGNNNCTG 2 cut(s) 565, 695
AoxI GGCC 4 cut(s) 82, 91, 347, 586
ApeKI GCWGC 5 cut(s) 21, 29, 101, 575, 842
ApoI RAATTY 2 cut(s) 383, 786
AsiGI ACCGGT 1 cut(s) 519
AspLEI GCGC 5 cut(s) 29, 41, 67, 122, 497
AspS9I GGNCC 3 cut(s) 347, 358, 613
AsuC2I CCSGG 2 cut(s) 211, 629
AsuHPI GGTGA 1 cut(s) 527
AsuII TTCGAA 1 cut(s) 635
AsuNHI GCTAGC 1 cut(s) 265
AvaII GGWCC 2 cut(s) 358, 613
BamHI GGATCC 1 cut(s) 454
BanI GGYRCC 1 cut(s) 494
BanII GRGCYC 3 cut(s) 189, 559, 862
BarI GAAGNNNNNNTAC 2 cut(s) 706, 738
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 5 cut(s) 33, 41, 113, 562, 829
BccI CCATC 3 cut(s) 754, 767, 805
BceAI ACGGC 3 cut(s) 106, 158, 230
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 2 cut(s) 195, 729
BciVI GTATCC 1 cut(s) 832
BcnI CCSGG 2 cut(s) 211, 629
BcoDI GTCTC 2 cut(s) 562, 653
BfaI CTAG 6 cut(s) 266, 395, 431, 846, 894, 905
BfmI CTRYAG 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 498
BfuI GTATCC 1 cut(s) 832
BglI GCCNNNNNGGC 2 cut(s) 90, 503
BlpI GCTNAGC 1 cut(s) 558
Bme1390I CCNGG 4 cut(s) 195, 211, 629, 729
Bme18I GGWCC 2 cut(s) 358, 613
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 3 cut(s) 347, 358, 613
BmiI GGNNCC 3 cut(s) 169, 456, 496
BmrFI CCNGG 4 cut(s) 195, 211, 629, 729
BmsI GCATC 3 cut(s) 394, 525, 682
BmtI GCTAGC 1 cut(s) 269
BpmI CTGGAG 2 cut(s) 209, 462
Bpu1102I GCTNAGC 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 635
BpuMI CCSGG 2 cut(s) 211, 629
BsaBI GATNNNNATC 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 495
BsaJI CCNNGG 4 cut(s) 501, 627, 728, 825
BsaWI WCCGGW 1 cut(s) 519
BsaXI ACNNNNNCTCC 2 cut(s) 159, 189
Bsc4I CCNNNNNNNGG 2 cut(s) 526, 734
Bse118I RCCGGY 2 cut(s) 122, 519
Bse1I ACTGG 1 cut(s) 656
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 2 cut(s) 195, 729
BseDI CCNNGG 4 cut(s) 501, 627, 728, 825
BseGI GGATG 1 cut(s) 816
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 526, 734
BseMII CTCAG 2 cut(s) 557, 572
BseNI ACTGG 1 cut(s) 656
BseRI GAGGAG 5 cut(s) 25, 224, 310, 465, 490
BseXI GCAGC 5 cut(s) 33, 41, 113, 562, 829
BseYI CCCAGC 1 cut(s) 854
BsgI GTGCAG 1 cut(s) 897
Bsh1236I CGCG 4 cut(s) 41, 65, 107, 120
BshFI GGCC 4 cut(s) 84, 93, 349, 588
BshNI GGYRCC 1 cut(s) 494
BshTI ACCGGT 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 5 cut(s) 123, 211, 350, 520, 629
BslFI GGGAC 2 cut(s) 74, 225
BslI CCNNNNNNNGG 2 cut(s) 526, 734
BsmAI GTCTC 2 cut(s) 562, 653
BsmFI GGGAC 2 cut(s) 74, 225
BsnI GGCC 4 cut(s) 84, 93, 349, 588
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 3 cut(s) 189, 559, 862
Bsp143I GATC 1 cut(s) 454
Bsp1720I GCTNAGC 1 cut(s) 558
BspACI CCGC 9 cut(s) 7, 24, 33, 81, 105, 151, 165, 171, 243
BspANI GGCC 4 cut(s) 84, 93, 349, 588
BspCNI CTCAG 2 cut(s) 558, 571
BspFNI CGCG 4 cut(s) 41, 65, 107, 120
BspLI GGNNCC 3 cut(s) 169, 456, 496
BspMAI CTGCAG 1 cut(s) 103
BspOI GCTAGC 1 cut(s) 269
BspPI GGATC 2 cut(s) 449, 462
BspQI GCTCTTC 1 cut(s) 177
BspT104I TTCGAA 1 cut(s) 635
BspT107I GGYRCC 1 cut(s) 494
BsrFI RCCGGY 2 cut(s) 122, 519
BsrI ACTGG 1 cut(s) 656
BssAI RCCGGY 2 cut(s) 122, 519
BssECI CCNNGG 4 cut(s) 501, 627, 728, 825
BssMI GATC 1 cut(s) 454
BssNI GRCGYC 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 825
Bst2UI CCWGG 2 cut(s) 195, 729
Bst6I CTCTTC 2 cut(s) 177, 287
BstACI GRCGYC 1 cut(s) 495
BstAPI GCANNNNNTGC 2 cut(s) 683, 692
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 5 cut(s) 37, 118, 124, 267, 779
BstDEI CTNAG 3 cut(s) 558, 566, 583
BstEII GGTNACC 1 cut(s) 515
BstF5I GGATG 1 cut(s) 816
BstFNI CGCG 4 cut(s) 41, 65, 107, 120
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 5 cut(s) 29, 41, 67, 122, 497
BstKTI GATC 1 cut(s) 457
BstMAI GTCTC 2 cut(s) 562, 653
BstMBI GATC 1 cut(s) 454
BstMWI GCNNNNNNNGC 9 cut(s) 21, 38, 90, 113, 271, 503, 683, 692, 851
BstNI CCWGG 2 cut(s) 195, 729
BstPI GGTNACC 1 cut(s) 515
BstSCI CCNGG 4 cut(s) 193, 209, 627, 727
BstSFI CTRYAG 1 cut(s) 99
BstUI CGCG 4 cut(s) 41, 65, 107, 120
BstV1I GCAGC 5 cut(s) 33, 41, 113, 562, 829
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
BsuI GTATCC 1 cut(s) 832
BsuRI GGCC 4 cut(s) 84, 93, 349, 588
BtrI CACGTC 1 cut(s) 157
BtsCI GGATG 1 cut(s) 816
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 5 cut(s) 37, 118, 124, 267, 779
CaiI CAGNNNCTG 2 cut(s) 565, 695
CfoI GCGC 5 cut(s) 29, 41, 67, 122, 497
Cfr10I RCCGGY 2 cut(s) 122, 519
Cfr13I GGNCC 3 cut(s) 347, 358, 613
CseI GACGC 2 cut(s) 71, 407
Csp6I GTAC 1 cut(s) 109
CspAI ACCGGT 1 cut(s) 519
CviAII CATG 2 cut(s) 275, 755
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 3 cut(s) 558, 566, 583
DinI GGCGCC 1 cut(s) 496
DpnI GATC 1 cut(s) 456
DpnII GATC 1 cut(s) 454
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 2 cut(s) 177, 287
EarI CTCTTC 2 cut(s) 177, 287
EciI GGCGGA 2 cut(s) 22, 180
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 825
Eco24I GRGCYC 3 cut(s) 189, 559, 862
Eco47I GGWCC 2 cut(s) 358, 613
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 2 cut(s) 311, 692
Eco91I GGTNACC 1 cut(s) 515
EcoICRI GAGCTC 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 358
EcoO65I GGTNACC 1 cut(s) 515
EcoRII CCWGG 2 cut(s) 193, 727
EcoT14I CCWWGG 1 cut(s) 825
EcoT38I GRGCYC 3 cut(s) 189, 559, 862
EgeI GGCGCC 1 cut(s) 496
EheI GGCGCC 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 825
FaeI CATG 2 cut(s) 278, 758
FaqI GGGAC 2 cut(s) 74, 225
FatI CATG 2 cut(s) 274, 754
FauI CCCGC 1 cut(s) 250
FblI GTMKAC 1 cut(s) 603
FokI GGATG 1 cut(s) 823
FriOI GRGCYC 3 cut(s) 189, 559, 862
FspBI CTAG 6 cut(s) 266, 395, 431, 846, 894, 905
GlaI GCGC 5 cut(s) 28, 40, 66, 121, 496
GsaI CCCAGC 1 cut(s) 858
GsuI CTGGAG 2 cut(s) 209, 462
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 4 cut(s) 84, 93, 349, 588
HapII CCGG 5 cut(s) 123, 211, 350, 520, 629
HgaI GACGC 2 cut(s) 71, 407
HhaI GCGC 5 cut(s) 29, 41, 67, 122, 497
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 2 cut(s) 278, 758
Hin6I GCGC 5 cut(s) 27, 39, 65, 120, 495
HinP1I GCGC 5 cut(s) 27, 39, 65, 120, 495
HinfI GANTC 5 cut(s) 198, 298, 424, 467, 482
HpaII CCGG 5 cut(s) 123, 211, 350, 520, 629
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 2 cut(s) 312, 604
Hpy188I TCNGA 4 cut(s) 77, 373, 567, 672
Hpy188III TCNNGA 2 cut(s) 226, 479
Hpy8I GTNNAC 2 cut(s) 312, 604
Hpy99I CGWCG 3 cut(s) 137, 307, 310
HpyAV CCTTC 6 cut(s) 40, 520, 626, 631, 667, 797
HpyCH4IV ACGT 2 cut(s) 156, 331
HpyCH4V TGCA 4 cut(s) 101, 538, 686, 878
HpyF10VI GCNNNNNNNGC 9 cut(s) 21, 38, 90, 113, 271, 503, 683, 692, 851
HpyF3I CTNAG 3 cut(s) 558, 566, 583
HpySE526I ACGT 2 cut(s) 156, 331
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 2 cut(s) 278, 758
HspAI GCGC 5 cut(s) 27, 39, 65, 120, 495
KasI GGCGCC 1 cut(s) 494
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Kzo9I GATC 1 cut(s) 454
LguI GCTCTTC 1 cut(s) 177
LmnI GCTCC 3 cut(s) 12, 167, 478
Lsp1109I GCAGC 5 cut(s) 33, 41, 113, 562, 829
LweI GCATC 3 cut(s) 394, 525, 682
MaeI CTAG 6 cut(s) 266, 395, 431, 846, 894, 905
MaeII ACGT 2 cut(s) 156, 331
MaeIII GTNAC 4 cut(s) 175, 285, 515, 868
MalI GATC 1 cut(s) 456
MboI GATC 1 cut(s) 454
MboII GAAGA 4 cut(s) 194, 304, 329, 477
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 3 cut(s) 189, 559, 862
MluCI AATT 3 cut(s) 257, 383, 786
Mly113I GGCGCC 1 cut(s) 495
MlyI GAGTC 1 cut(s) 307
MroNI GCCGGC 1 cut(s) 122
MseI TTAA 2 cut(s) 363, 922
MspA1I CMGCKG 1 cut(s) 24
MspI CCGG 5 cut(s) 123, 211, 350, 520, 629
MspR9I CCNGG 4 cut(s) 195, 211, 629, 729
MvaI CCWGG 2 cut(s) 195, 729
MvnI CGCG 4 cut(s) 41, 65, 107, 120
MwoI GCNNNNNNNGC 9 cut(s) 21, 38, 90, 113, 271, 503, 683, 692, 851
NaeI GCCGGC 1 cut(s) 124
NarI GGCGCC 1 cut(s) 495
NciI CCSGG 2 cut(s) 211, 629
NdeII GATC 1 cut(s) 454
NgoMIV GCCGGC 1 cut(s) 122
NheI GCTAGC 1 cut(s) 265
NlaIII CATG 2 cut(s) 278, 758
NlaIV GGNNCC 3 cut(s) 169, 456, 496
NmeAIII GCCGAG 2 cut(s) 250, 482
NmuCI GTSAC 3 cut(s) 175, 515, 868
NspV TTCGAA 1 cut(s) 635
PciSI GCTCTTC 1 cut(s) 177
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PdiI GCCGGC 1 cut(s) 124
PfeI GAWTC 4 cut(s) 198, 424, 467, 482
PflMI CCANNNNNTGG 1 cut(s) 734
PfoI TCCNGGA 1 cut(s) 193
PinAI ACCGGT 1 cut(s) 519
PleI GAGTC 1 cut(s) 306
PluTI GGCGCC 1 cut(s) 498
PpsI GAGTC 1 cut(s) 306
PpuMI RGGWCCY 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 193, 727
PspEI GGTNACC 1 cut(s) 515
PspFI CCCAGC 1 cut(s) 854
PspGI CCWGG 2 cut(s) 193, 727
PspN4I GGNNCC 3 cut(s) 169, 456, 496
PspPI GGNCC 3 cut(s) 347, 358, 613
PspPPI RGGWCCY 1 cut(s) 358
PstI CTGCAG 1 cut(s) 103
PstNI CAGNNNCTG 2 cut(s) 565, 695
PsuI RGATCY 1 cut(s) 454
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SacI GAGCTC 1 cut(s) 189
SapI GCTCTTC 1 cut(s) 177
SaqAI TTAA 2 cut(s) 363, 922
Sau3AI GATC 1 cut(s) 454
Sau96I GGNCC 3 cut(s) 347, 358, 613
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 4 cut(s) 195, 211, 629, 729
SduI GDGCHC 3 cut(s) 189, 559, 862
SfaNI GCATC 3 cut(s) 394, 525, 682
SfcI CTRYAG 1 cut(s) 99
SfiI GGCCNNNNNGGCC 1 cut(s) 90
SfoI GGCGCC 1 cut(s) 496
SfuI TTCGAA 1 cut(s) 635
SinI GGWCC 2 cut(s) 358, 613
Sse9I AATT 3 cut(s) 257, 383, 786
SsiI CCGC 9 cut(s) 7, 24, 33, 81, 105, 151, 165, 171, 243
SspDI GGCGCC 1 cut(s) 494
SspMI CTAG 6 cut(s) 266, 395, 431, 846, 894, 905
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 4 cut(s) 193, 209, 627, 727
StyI CCWWGG 1 cut(s) 825
TaiI ACGT 2 cut(s) 159, 334
TaqI TCGA 3 cut(s) 144, 545, 635
TasI AATT 3 cut(s) 257, 383, 786
TauI GCSGC 6 cut(s) 27, 35, 84, 107, 153, 173
TfiI GAWTC 4 cut(s) 198, 424, 467, 482
Tru1I TTAA 2 cut(s) 363, 922
Tru9I TTAA 2 cut(s) 363, 922
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 3 cut(s) 175, 515, 868
TseI GCWGC 5 cut(s) 21, 29, 101, 575, 842
Tsp45I GTSAC 3 cut(s) 175, 515, 868
TspDTI ATGAA 1 cut(s) 296
TspGWI ACGGA 5 cut(s) 121, 194, 227, 291, 342
TspRI CASTG 1 cut(s) 103
Van91I CCANNNNNTGG 1 cut(s) 734
VpaK11BI GGWCC 2 cut(s) 358, 613
XapI RAATTY 2 cut(s) 383, 786
XmiI GTMKAC 1 cut(s) 603
XspI CTAG 6 cut(s) 266, 395, 431, 846, 894, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.