Rroxscaffold_2G00149570

Belongs to the hexokinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010710
Physical Location & Seq
Forward (+)
87125331 .. 87129811
4481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149570.1

Sequence Viewer

Length: 1428 bp
ATGGGGAAGGTGGCGGTGGGCGCGGCGGTGGTGTGCGCGGCGGTGACGTGTGCGGCGGCGGCGCTGCTGGTGCGGCGTCGTTTGAAGAGCTCGAGGAGGTGGGCCCGGGCGATGGCGATACTGAAGGAGTTTGAGGACAAGTGTGACACGCCGATTGGGAAGCTCCGGCAGGTCGCCGACGCCATGACGGTGGAGATGCACGCCGGGCTTGCGTCCGAGGGCGGCAGCAAGTTGAAGATGCTCATCAGCTATGTTGATAACCTCCCCACTGGGGATGAGAAAGGTCTATTTTATGCATTAGACCTTGGTGGGACAAACTTCCGTGTCCTGCGTGTACAGCTTGGTGGGAAGGAAAAGCGTGTTATTAAACAAGAATTTGACGAAGTTTCAATTCCACCACATTTGATGACTGGGACATCAGAAGGTTTATTTGATTTTATTGCTGAAGCACTAGCAAAATTTGTTGCTACTGAAGGAGAAGGTTTTCATCCTGCACCTGGTAGGCTAAGGGAGCTGGGTTTTACCTTCTCATTTCCCGTTGACCAAGATGTGGTGGCGGAGCTCACCAAATCAGTGGAAAAAATTGGCCTGGATATGCGTGTTACAGCTTTGGTTAATGATACAATTGGGACATTAGCTGGAGGTAGGTACCACAATCCGGATGCGATTGCTGCTGTCATTCTGGGCACTGGAACAAATGCTGCTTATGTAGAACGCGCACATGCCATTCCAAAATGGCATGGTCTTCTACCTAAATCAGGAGAGATGGTAATCAACATGGAGTGGGGTAACTTTCGTTCATCACACCTTCCCCTAACAGAATATGATCAAGCCCTTGATGCTGAGAGTTTGAACCCTGGAGATCAAATCTTTGAGAAGATGATTTCTGGGATGTATTTAGGAGAGATTGTGCGCAGAGTTTTGTTGAAGATGGCTGAAGAAGCTGCCTTTTTTGGTGATACTGTTCCACCCAAACTGAAAGTTCCTTTCATATTAAGGACTCCTGACATGTCTGCAATGCATCATGACTCATCTCTGGATCTGAAAGTAGTTGGGACCAAACTCAAGAATATCCTAGAGATCTCCAATACATCACTGAAAGTGAGAAAGGCTGTTGTGGCGTTATGTGACATAGTTGCCACTCGTGGGGCCCGCCTCTCCGCTGCTGGGATCCTGGGAGTCCTTAAGAAGTTGGGTAGAGACACAGTAAAGGATGGAGAGAAGCACAAGTCAGTGGTAGCAATGGATGGTGGCTTGTTTGAGCATTACACTGAATTCCGCGTCTGCATGGAGAGTACTCTTTATGAGTTGTTGGGTGATGAAGTTGCTGACCACATCTCGGTTGAGCACTCAAATGATGGGTCCGGAATTGGAGCTGCTCTCCTGGCAGCCTCTCACTCCCATTACCTTGGGGCAGAGGAGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001047 GO:0001067 GO:0001678 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004340 GO:0004396 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005773 GO:0005774 GO:0005829 GO:0005975 GO:0005996 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006355 GO:0006357 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0008865 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009536 GO:0009743 GO:0009746 GO:0009747 GO:0009749 GO:0009756 GO:0009757 GO:0009889 GO:0009987 GO:0010033 GO:0010118 GO:0010148 GO:0010182 GO:0010255 GO:0010468 GO:0010556 GO:0012501 GO:0016020 GO:0016021 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019158 GO:0019200 GO:0019219 GO:0019222 GO:0019318 GO:0019320 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019725 GO:0019752 GO:0019867 GO:0023052 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031323 GO:0031326 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032592 GO:0032787 GO:0032991 GO:0033500 GO:0034284 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042044 GO:0042221 GO:0042592 GO:0042593 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044212 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0046031 GO:0046034 GO:0046365 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046872 GO:0046914 GO:0046939 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051156 GO:0051171 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051252 GO:0051716 GO:0055082 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080090 GO:0090332 GO:0090407 GO:0097159 GO:0098573 GO:0098588 GO:0098805 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901700 GO:1901701 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00052 ko00500 ko00520 ko00521 ko00524 ko01100 ko01110 ko01120 ko01130 ko01200 ko04066 ko04910 ko04930 ko04973 ko05230 map00010 map00051 map00052 map00500 map00520 map00521 map00524 map01100 map01110 map01120 map01130 map01200 map04066 map04910 map04930 map04973 map05230
Pfam Domains
Protein Families
Hexokinase_1 Hexokinase_2

Protein Analysis

475

Amino Acids

51.37

Weight (kDa)

6.13

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hexokinase_1 PF00349 41 - 183 2.4e-45 Hexokinase
Hexokinase_1 PF00349 181 - 217 8.9e-07 Hexokinase
Hexokinase_2 PF03727 224 - 464 2.7e-84 Hexokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010710)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19860 AT2G19860 AT4G29130
fragaria_vesca FvH4_1g06270
malus_domestica MD02G1066500.v1.1 MD15G1197000.v1.1
prunus_persica Prupe.7G218800_v2.0.a1
pyrus_communis pycom02g05260 pycom15g17600
rosa_chinensis RchiOBHm_Chr2g0091971
rosa_laevigata RLG00000016267
rosa_multiflora Rmu_sc0009216.1_g000003 Rmu_sc0017125.1_g000004
rosa_roxburghii Rroxscaffold_2G00149570
rosa_rugosa Rorug02G0021900
rosa_samantha Rh2AG067600 Rh2BG066900 Rh2CG068200 Rh2DG066700
rosa_wichuraiana Rw2G005340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 914
Acc36I ACCTGC 1 cut(s) 160
Acc65I GGTACC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 648
AccB7I CCANNNNNTGG 1 cut(s) 550
AccII CGCG 4 cut(s) 23, 38, 717, 1281
AccIII TCCGGA 2 cut(s) 658, 1364
AclWI GGATC 3 cut(s) 1047, 1165, 1178
AcsI RAATTY 3 cut(s) 374, 458, 1274
AcuI CTGAAG 4 cut(s) 143, 465, 492, 957
AcyI GRCGYC 2 cut(s) 76, 180
AfaI GTAC 3 cut(s) 336, 650, 1297
AfiI CCNNNNNNNGG 9 cut(s) 112, 271, 497, 550, 658, 758, 1146, 1167, 1339
AflII CTTAAG 1 cut(s) 1184
AflIII ACRYGT 2 cut(s) 47, 1008
AgsI TTSAA 5 cut(s) 85, 235, 390, 853, 928
AjiI CACGTC 1 cut(s) 48
AjnI CCWGG 5 cut(s) 496, 588, 856, 1173, 1383
Alw21I GWGCWC 3 cut(s) 92, 564, 1350
Alw26I GTCTC 1 cut(s) 1194
AlwI GGATC 3 cut(s) 1047, 1165, 1178
Ama87I CYCGRG 2 cut(s) 91, 105
Aor13HI TCCGGA 2 cut(s) 658, 1364
AoxI GGCC 3 cut(s) 102, 586, 1149
ApaI GGGCCC 2 cut(s) 106, 1153
ApeKI GCWGC 8 cut(s) 64, 225, 671, 701, 944, 1163, 1376, 1388
ApoI RAATTY 3 cut(s) 374, 458, 1274
ArsI GACNNNNNNTTYG 2 cut(s) 1346, 1378
Asp700I GAANNNNTTC 1 cut(s) 483
Asp718I GGTACC 1 cut(s) 648
AspLEI GCGC 5 cut(s) 23, 38, 64, 719, 915
AspS9I GGNCC 6 cut(s) 102, 103, 1056, 1149, 1150, 1362
AsuC2I CCSGG 3 cut(s) 106, 107, 205
AsuHPI GGTGA 4 cut(s) 55, 556, 968, 1328
AvaI CYCGRG 2 cut(s) 91, 105
AvaII GGWCC 2 cut(s) 1056, 1362
BaeGI GKGCMC 3 cut(s) 106, 689, 1153
BamHI GGATCC 1 cut(s) 1170
BanI GGYRCC 1 cut(s) 648
BanII GRGCYC 4 cut(s) 92, 106, 564, 1153
BauI CACGAG 1 cut(s) 1143
BbsI GAAGAC 1 cut(s) 737
Bbv12I GWGCWC 3 cut(s) 92, 564, 1350
BbvI GCAGC 8 cut(s) 51, 237, 658, 688, 931, 1150, 1363, 1400
BccI CCATC 6 cut(s) 106, 760, 925, 1208, 1241, 1352
BcgI CGANNNNNNTGC 1 cut(s) 1404
BciT130I CCWGG 5 cut(s) 498, 590, 858, 1175, 1385
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 3 cut(s) 106, 107, 205
BcoDI GTCTC 1 cut(s) 1194
BfaI CTAG 2 cut(s) 452, 1076
BfoI RGCGCY 1 cut(s) 65
BfrI CTTAAG 1 cut(s) 1184
BfuAI ACCTGC 1 cut(s) 160
BglII AGATCT 1 cut(s) 1080
BmcAI AGTACT 1 cut(s) 1297
Bme1390I CCNGG 8 cut(s) 106, 107, 205, 498, 590, 858, 1175, 1385
Bme18I GGWCC 2 cut(s) 1056, 1362
BmeT110I CYCGRG 2 cut(s) 91, 105
BmgBI CACGTC 1 cut(s) 48
BmgT120I GGNCC 6 cut(s) 102, 103, 1056, 1149, 1150, 1362
BmiI GGNNCC 7 cut(s) 104, 650, 1057, 1150, 1151, 1172, 1363
BmrFI CCNGG 8 cut(s) 106, 107, 205, 498, 590, 858, 1175, 1385
BmrI ACTGGG 2 cut(s) 279, 420
BmsI GCATC 5 cut(s) 186, 228, 652, 829, 1030
BmuI ACTGGG 2 cut(s) 279, 420
BpiI GAAGAC 1 cut(s) 737
BplI GAGNNNNNCTC 2 cut(s) 1365, 1397
BpmI CTGGAG 2 cut(s) 660, 879
Bpu10I CCTNAGC 1 cut(s) 506
BpuEI CTTGAG 1 cut(s) 1049
BpuMI CCSGG 3 cut(s) 106, 107, 205
BsaBI GATNNNNATC 2 cut(s) 242, 770
BsaHI GRCGYC 2 cut(s) 76, 180
BsaJI CCNNGG 6 cut(s) 105, 216, 304, 856, 1174, 1408
BsaWI WCCGGW 2 cut(s) 658, 1364
BsaXI ACNNNNNCTCC 4 cut(s) 753, 783, 894, 924
Bsc4I CCNNNNNNNGG 9 cut(s) 112, 271, 497, 550, 658, 758, 1146, 1167, 1339
Bse1I ACTGG 3 cut(s) 274, 415, 694
Bse3DI GCAATG 2 cut(s) 1023, 1248
Bse8I GATNNNNATC 2 cut(s) 242, 770
BseAI TCCGGA 2 cut(s) 658, 1364
BseBI CCWGG 5 cut(s) 498, 590, 858, 1175, 1385
BseDI CCNNGG 6 cut(s) 105, 216, 304, 856, 1174, 1408
BseGI GGATG 6 cut(s) 280, 487, 667, 897, 1219, 1252
BseJI GATNNNNATC 2 cut(s) 242, 770
BseLI CCNNNNNNNGG 9 cut(s) 112, 271, 497, 550, 658, 758, 1146, 1167, 1339
BseMI GCAATG 2 cut(s) 1023, 1248
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 3 cut(s) 274, 415, 694
BseRI GAGGAG 1 cut(s) 109
BseSI GKGCMC 3 cut(s) 106, 689, 1153
BseXI GCAGC 8 cut(s) 51, 237, 658, 688, 931, 1150, 1363, 1400
BseYI CCCAGC 2 cut(s) 514, 1166
BsgI GTGCAG 1 cut(s) 477
Bsh1236I CGCG 4 cut(s) 23, 38, 717, 1281
BshFI GGCC 3 cut(s) 104, 588, 1151
BshNI GGYRCC 1 cut(s) 648
BsiHKAI GWGCWC 3 cut(s) 92, 564, 1350
BsiHKCI CYCGRG 2 cut(s) 91, 105
BsiSI CCGG 5 cut(s) 106, 166, 204, 659, 1365
BslFI GGGAC 4 cut(s) 325, 427, 643, 1069
BslI CCNNNNNNNGG 9 cut(s) 112, 271, 497, 550, 658, 758, 1146, 1167, 1339
BsmAI GTCTC 1 cut(s) 1194
BsmFI GGGAC 4 cut(s) 325, 427, 643, 1069
BsnI GGCC 3 cut(s) 104, 588, 1151
BsoBI CYCGRG 2 cut(s) 91, 105
Bsp120I GGGCCC 2 cut(s) 102, 1149
Bsp1286I GDGCHC 6 cut(s) 92, 106, 564, 689, 1153, 1350
Bsp13I TCCGGA 2 cut(s) 658, 1364
Bsp1407I TGTACA 1 cut(s) 334
Bsp143I GATC 5 cut(s) 826, 862, 1039, 1080, 1170
BspANI GGCC 3 cut(s) 104, 588, 1151
BspCNI CTCAG 1 cut(s) 835
BspEI TCCGGA 2 cut(s) 658, 1364
BspFNI CGCG 4 cut(s) 23, 38, 717, 1281
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 7 cut(s) 104, 650, 1057, 1150, 1151, 1172, 1363
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 3 cut(s) 1047, 1165, 1178
BspQI GCTCTTC 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 648
BspTI CTTAAG 1 cut(s) 1184
BsrDI GCAATG 2 cut(s) 1023, 1248
BsrGI TGTACA 1 cut(s) 334
BsrI ACTGG 3 cut(s) 274, 415, 694
BssECI CCNNGG 6 cut(s) 105, 216, 304, 856, 1174, 1408
BssMI GATC 5 cut(s) 826, 862, 1039, 1080, 1170
BssNI GRCGYC 2 cut(s) 76, 180
BssSI CACGAG 1 cut(s) 1143
BssT1I CCWWGG 2 cut(s) 304, 1408
Bst2BI CACGAG 1 cut(s) 1143
Bst2UI CCWGG 5 cut(s) 498, 590, 858, 1175, 1385
Bst4CI ACNGT 3 cut(s) 190, 964, 1207
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 2 cut(s) 76, 180
BstAFI CTTAAG 1 cut(s) 1184
BstAUI TGTACA 1 cut(s) 334
BstC8I GCNNGC 3 cut(s) 201, 210, 1153
BstDEI CTNAG 2 cut(s) 506, 843
BstENI CCTNNNNNAGG 1 cut(s) 756
BstF5I GGATG 6 cut(s) 280, 487, 667, 897, 1219, 1252
BstFNI CGCG 4 cut(s) 23, 38, 717, 1281
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 5 cut(s) 23, 38, 64, 719, 915
BstKTI GATC 5 cut(s) 829, 865, 1042, 1083, 1173
BstMAI GTCTC 1 cut(s) 1194
BstMBI GATC 5 cut(s) 826, 862, 1039, 1080, 1170
BstNI CCWGG 5 cut(s) 498, 590, 858, 1175, 1385
BstNSI RCATGY 2 cut(s) 725, 1012
BstSCI CCNGG 8 cut(s) 104, 105, 203, 496, 588, 856, 1173, 1383
BstSLI GKGCMC 3 cut(s) 106, 689, 1153
BstUI CGCG 4 cut(s) 23, 38, 717, 1281
BstV1I GCAGC 8 cut(s) 51, 237, 658, 688, 931, 1150, 1363, 1400
BstV2I GAAGAC 1 cut(s) 737
BstX2I RGATCY 3 cut(s) 1039, 1080, 1170
BstXI CCANNNNNNTGG 3 cut(s) 190, 574, 1409
BstYI RGATCY 3 cut(s) 1039, 1080, 1170
BsuRI GGCC 3 cut(s) 104, 588, 1151
BtgZI GCGATG 1 cut(s) 125
BtrI CACGTC 1 cut(s) 48
BtsCI GGATG 6 cut(s) 280, 487, 667, 897, 1219, 1252
BtsIMutI CAGTG 6 cut(s) 267, 579, 687, 1094, 1239, 1269
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 3 cut(s) 201, 210, 1153
CciI TCATGA 1 cut(s) 1024
CfoI GCGC 5 cut(s) 23, 38, 64, 719, 915
Cfr13I GGNCC 6 cut(s) 102, 103, 1056, 1149, 1150, 1362
Cfr9I CCCGGG 1 cut(s) 105
CseI GACGC 4 cut(s) 65, 188, 201, 1270
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 3 cut(s) 335, 649, 1296
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CviAII CATG 7 cut(s) 184, 722, 740, 778, 1009, 1025, 1288
CviQI GTAC 3 cut(s) 335, 649, 1296
DdeI CTNAG 2 cut(s) 506, 843
DpnI GATC 5 cut(s) 828, 864, 1041, 1082, 1172
DpnII GATC 5 cut(s) 826, 862, 1039, 1080, 1170
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
EciI GGCGGA 1 cut(s) 572
Ecl136II GAGCTC 2 cut(s) 90, 562
Eco130I CCWWGG 2 cut(s) 304, 1408
Eco24I GRGCYC 4 cut(s) 92, 106, 564, 1153
Eco47I GGWCC 2 cut(s) 1056, 1362
Eco53kI GAGCTC 2 cut(s) 90, 562
Eco57I CTGAAG 4 cut(s) 143, 465, 492, 957
Eco88I CYCGRG 2 cut(s) 91, 105
EcoICRI GAGCTC 2 cut(s) 90, 562
EcoNI CCTNNNNNAGG 1 cut(s) 756
EcoO109I RGGNCCY 1 cut(s) 1149
EcoRI GAATTC 1 cut(s) 1274
EcoRII CCWGG 5 cut(s) 496, 588, 856, 1173, 1383
EcoT14I CCWWGG 2 cut(s) 304, 1408
EcoT22I ATGCAT 2 cut(s) 298, 1023
EcoT38I GRGCYC 4 cut(s) 92, 106, 564, 1153
ErhI CCWWGG 2 cut(s) 304, 1408
FaeI CATG 7 cut(s) 187, 725, 743, 781, 1012, 1028, 1291
FaqI GGGAC 4 cut(s) 325, 427, 643, 1069
FatI CATG 7 cut(s) 183, 721, 739, 777, 1008, 1024, 1287
FauI CCCGC 1 cut(s) 1160
FbaI TGATCA 1 cut(s) 826
FokI GGATG 6 cut(s) 287, 474, 674, 904, 1226, 1259
FriOI GRGCYC 4 cut(s) 92, 106, 564, 1153
FspBI CTAG 2 cut(s) 452, 1076
FspI TGCGCA 1 cut(s) 914
GlaI GCGC 5 cut(s) 22, 37, 63, 718, 914
GsaI CCCAGC 2 cut(s) 518, 1170
GsuI CTGGAG 2 cut(s) 660, 879
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 3 cut(s) 104, 588, 1151
HapII CCGG 5 cut(s) 106, 166, 204, 659, 1365
HgaI GACGC 4 cut(s) 65, 188, 201, 1270
HhaI GCGC 5 cut(s) 23, 38, 64, 719, 915
Hin1I GRCGYC 2 cut(s) 76, 180
Hin1II CATG 7 cut(s) 187, 725, 743, 781, 1012, 1028, 1291
Hin6I GCGC 5 cut(s) 21, 36, 62, 717, 913
HinP1I GCGC 5 cut(s) 21, 36, 62, 717, 913
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 4 cut(s) 1000, 1028, 1179, 1421
HpaII CCGG 5 cut(s) 106, 166, 204, 659, 1365
HphI GGTGA 4 cut(s) 55, 556, 968, 1328
Hpy166II GTNNAC 2 cut(s) 335, 541
Hpy188I TCNGA 3 cut(s) 217, 421, 1044
Hpy188III TCNNGA 7 cut(s) 659, 759, 1004, 1025, 1037, 1066, 1365
Hpy8I GTNNAC 2 cut(s) 335, 541
Hpy99I CGWCG 2 cut(s) 81, 182
HpyAV CCTTC 7 cut(s) 118, 343, 416, 467, 473, 535, 818
HpyCH4III ACNGT 3 cut(s) 190, 964, 1207
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 6 cut(s) 199, 296, 494, 1016, 1021, 1287
HpyF3I CTNAG 2 cut(s) 506, 843
HpySE526I ACGT 1 cut(s) 47
Hsp92I GRCGYC 2 cut(s) 76, 180
Hsp92II CATG 7 cut(s) 187, 725, 743, 781, 1012, 1028, 1291
HspAI GCGC 5 cut(s) 21, 36, 62, 717, 913
Kpn2I TCCGGA 2 cut(s) 658, 1364
KpnI GGTACC 1 cut(s) 652
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 5 cut(s) 826, 862, 1039, 1080, 1170
LguI GCTCTTC 1 cut(s) 80
LmnI GCTCC 4 cut(s) 168, 511, 559, 1373
Lsp1109I GCAGC 8 cut(s) 51, 237, 658, 688, 931, 1150, 1363, 1400
LweI GCATC 5 cut(s) 186, 228, 652, 829, 1030
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 2 cut(s) 452, 1076
MaeII ACGT 1 cut(s) 47
MaeIII GTNAC 5 cut(s) 43, 143, 601, 788, 1127
MalI GATC 5 cut(s) 828, 864, 1041, 1082, 1172
MboI GATC 5 cut(s) 826, 862, 1039, 1080, 1170
MboII GAAGA 6 cut(s) 97, 247, 737, 889, 940, 950
MfeI CAATTG 1 cut(s) 624
MflI RGATCY 3 cut(s) 1039, 1080, 1170
MhlI GDGCHC 6 cut(s) 92, 106, 564, 689, 1153, 1350
MluCI AATT 7 cut(s) 374, 390, 458, 582, 624, 1274, 1368
MlyI GAGTC 3 cut(s) 994, 1022, 1188
MnlI CCTC 9 cut(s) 87, 90, 127, 211, 272, 635, 1166, 1402, 1411
Mph1103I ATGCAT 2 cut(s) 298, 1023
MroI TCCGGA 2 cut(s) 658, 1364
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 4 cut(s) 366, 615, 995, 1185
MslI CAYNNNNRTG 2 cut(s) 188, 1353
MspA1I CMGCKG 1 cut(s) 1163
MspCI CTTAAG 1 cut(s) 1184
MspI CCGG 5 cut(s) 106, 166, 204, 659, 1365
MspR9I CCNGG 8 cut(s) 106, 107, 205, 498, 590, 858, 1175, 1385
MunI CAATTG 1 cut(s) 624
MvaI CCWGG 5 cut(s) 498, 590, 858, 1175, 1385
MvnI CGCG 4 cut(s) 23, 38, 717, 1281
NciI CCSGG 3 cut(s) 106, 107, 205
NdeII GATC 5 cut(s) 826, 862, 1039, 1080, 1170
NlaIII CATG 7 cut(s) 187, 725, 743, 781, 1012, 1028, 1291
NlaIV GGNNCC 7 cut(s) 104, 650, 1057, 1150, 1151, 1172, 1363
NmuCI GTSAC 3 cut(s) 43, 143, 1127
NsbI TGCGCA 1 cut(s) 914
NsiI ATGCAT 2 cut(s) 298, 1023
NspI RCATGY 2 cut(s) 725, 1012
PaeR7I CTCGAG 1 cut(s) 91
PagI TCATGA 1 cut(s) 1024
PciI ACATGT 1 cut(s) 1008
PciSI GCTCTTC 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 483
PflMI CCANNNNNTGG 1 cut(s) 550
PleI GAGTC 3 cut(s) 994, 1022, 1187
PpsI GAGTC 3 cut(s) 994, 1022, 1187
PscI ACATGT 1 cut(s) 1008
Psp124BI GAGCTC 2 cut(s) 92, 564
Psp6I CCWGG 5 cut(s) 496, 588, 856, 1173, 1383
PspFI CCCAGC 2 cut(s) 514, 1166
PspGI CCWGG 5 cut(s) 496, 588, 856, 1173, 1383
PspN4I GGNNCC 7 cut(s) 104, 650, 1057, 1150, 1151, 1172, 1363
PspOMI GGGCCC 2 cut(s) 102, 1149
PspPI GGNCC 6 cut(s) 102, 103, 1056, 1149, 1150, 1362
PspXI VCTCGAGB 1 cut(s) 91
PsrI GAACNNNNNNTAC 2 cut(s) 781, 813
PsuI RGATCY 3 cut(s) 1039, 1080, 1170
RsaI GTAC 3 cut(s) 336, 650, 1297
RsaNI GTAC 3 cut(s) 335, 649, 1296
RseI CAYNNNNRTG 2 cut(s) 188, 1353
SacI GAGCTC 2 cut(s) 92, 564
SapI GCTCTTC 1 cut(s) 80
SaqAI TTAA 4 cut(s) 366, 615, 995, 1185
Sau3AI GATC 5 cut(s) 826, 862, 1039, 1080, 1170
Sau96I GGNCC 6 cut(s) 102, 103, 1056, 1149, 1150, 1362
ScaI AGTACT 1 cut(s) 1297
SchI GAGTC 3 cut(s) 994, 1022, 1188
ScrFI CCNGG 8 cut(s) 106, 107, 205, 498, 590, 858, 1175, 1385
SduI GDGCHC 6 cut(s) 92, 106, 564, 689, 1153, 1350
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 5 cut(s) 186, 228, 652, 829, 1030
Sfr274I CTCGAG 1 cut(s) 91
SinI GGWCC 2 cut(s) 1056, 1362
SlaI CTCGAG 1 cut(s) 91
SmaI CCCGGG 1 cut(s) 107
SmiMI CAYNNNNRTG 2 cut(s) 188, 1353
SmlI CTYRAG 3 cut(s) 91, 1064, 1184
SmoI CTYRAG 3 cut(s) 91, 1064, 1184
SrfI GCCCGGGC 1 cut(s) 107
Sse9I AATT 7 cut(s) 374, 390, 458, 582, 624, 1274, 1368
SspMI CTAG 2 cut(s) 452, 1076
SstI GAGCTC 2 cut(s) 92, 564
StyD4I CCNGG 8 cut(s) 104, 105, 203, 496, 588, 856, 1173, 1383
StyI CCWWGG 2 cut(s) 304, 1408
TaaI ACNGT 3 cut(s) 190, 964, 1207
TaiI ACGT 1 cut(s) 50
TaqI TCGA 1 cut(s) 92
TasI AATT 7 cut(s) 374, 390, 458, 582, 624, 1274, 1368
TatI WGTACW 2 cut(s) 334, 1295
TauI GCSGC 7 cut(s) 26, 41, 56, 59, 62, 76, 225
Tru1I TTAA 4 cut(s) 366, 615, 995, 1185
Tru9I TTAA 4 cut(s) 366, 615, 995, 1185
TscAI CASTG 6 cut(s) 274, 579, 694, 1101, 1239, 1276
TseFI GTSAC 3 cut(s) 43, 143, 1127
TseI GCWGC 8 cut(s) 64, 225, 671, 701, 944, 1163, 1376, 1388
Tsp45I GTSAC 3 cut(s) 43, 143, 1127
TspDTI ATGAA 4 cut(s) 476, 789, 979, 1335
TspGWI ACGGA 1 cut(s) 311
TspMI CCCGGG 1 cut(s) 105
TspRI CASTG 6 cut(s) 274, 579, 694, 1101, 1239, 1276
Van91I CCANNNNNTGG 1 cut(s) 550
Vha464I CTTAAG 1 cut(s) 1184
VpaK11BI GGWCC 2 cut(s) 1056, 1362
XagI CCTNNNNNAGG 1 cut(s) 756
XapI RAATTY 3 cut(s) 374, 458, 1274
XceI RCATGY 2 cut(s) 725, 1012
XhoI CTCGAG 1 cut(s) 91
XmaI CCCGGG 1 cut(s) 105
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 2 cut(s) 452, 1076
ZrmI AGTACT 1 cut(s) 1297
Zsp2I ATGCAT 2 cut(s) 298, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.