Rh2DG066700

Belongs to the hexokinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010710
Physical Location & Seq
Reverse (-)
4993163 .. 4996832
3670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG066700.1

Sequence Viewer

Length: 1497 bp
ATGGGGAAGGTGGCGGTGGGCGCGGCGGTGGTGTGCGCGGCGGTGACGTGCGCGGCGGCGGCGCTGCTGGTGCGGCGTCGTTTGAAGAGCTCGAGGAGGTGGGCCCGGGCGATGGCGATACTGAAGGAGTTTGAGGACAAGTGTGACACGCCGATTGGGAAGCTCCGGCAGGTCGCCGACGCCATGACGGTGGAGATGCACGCCGGGCTTGCGTCCGAGGGCGGCAGCAAGTTGAAGATGCTCATCAGCTATGTTGATAACCTCCCCACTGGGGATGAGAAAGGTCTATTTTATGCATTAGACCTTGGTGGGACAAACTTCCGTGTCCTGCGTGTACAGCTTGGTGGGAAGGAAAAGCGTGTTATTAAACAAGAATTTGATGAAGTTTCAATTCCACCACATTTGATGACTGGGACATCAGAAGGATTATTTGATTTTATTGCTGAAGCACTAGCAAAATTTGTTGCTACTGAAGGAGAAGGTTTTCATCCTGCACCTGGTAGGCTAAGGGAGCTGGGTTTTACCTTCTCCTTTCCCGTATGGCAAACATCAATTGCTTCCGGGACCCTTATGAAGTGGACAAAAGGATTCAGTATTGAAGATGCGGTTGACCAAGATGTGGTGGCGGAGCTCACCAAATCAGTGGAAAAAATTGGCCTGGATATGCGTGTTACAGCTTTGGTTAATGATACAATTGGGACATTAGCTGGAGGTAGGTACCACAATCCGGATGCGATTGCTGCTGTCATTCTGGGCACTGGAACAAATGCCGCTTATGTAGAACGCGCACATGCCATTCCAAAATGGCATGGTCTTCTACCTAAATCAGGAGAGATGGTAATCAACATGGAGTGGGGTAACTTTCGTTCATCACACCTTCCCCTAACAGAATATGATCAAGCCCTTGATGCTGAGAGTTTGAACCCTGGAGATCAAATCTTTGAGAAGATTATTTCTGGGATGTATTTAGGAGAGATTGTGCGCAGAGTTTTGTTGAAGATGGCTGAAGAAGCTGCCTTTTTTGGTGATACTGTTCCACCCAAACTGAAAGTTCCTTTCATATTAAGGACTCCTGATATGTCCGCAATGCATCATGACTCATCTCTGGATCTGAAAATAGTTGGGACCAAACTCAAGGATATCCTAGAGATCTCCAATACATCACTGAAAGTGAGAAAGGCTGTTGTGGCGTTATGTGACATAGTCGCCACTCGTGGGGCCCGCCTTTCCGCTGCTGGGATCCTGGGAGTCCTTAAGAAGTTGGGTAGAGACACAGTAAAGGATGGAGAGAAGCACAAGTCAGTGGTAGCAATGGATGGTGGCTTGTTTGAGCATTACACTGAATTCCGCGTCTGCATGGAGAGTACTCTTTATGAGTTGTTGGGTGATGAAGTTGCTGAGCACATCTCGGTTGAGCACTCAAATGATGGGTCCGGAATTGGAGCTGCTCTCCTGGCAGCCTCTCACTCCCATTACCTTGGGGCAGAGGAGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001047 GO:0001067 GO:0001678 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004340 GO:0004396 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005773 GO:0005774 GO:0005829 GO:0005975 GO:0005996 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006355 GO:0006357 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0008865 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009536 GO:0009743 GO:0009746 GO:0009747 GO:0009749 GO:0009756 GO:0009757 GO:0009889 GO:0009987 GO:0010033 GO:0010118 GO:0010148 GO:0010182 GO:0010255 GO:0010468 GO:0010556 GO:0012501 GO:0016020 GO:0016021 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019158 GO:0019200 GO:0019219 GO:0019222 GO:0019318 GO:0019320 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019725 GO:0019752 GO:0019867 GO:0023052 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031323 GO:0031326 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032592 GO:0032787 GO:0032991 GO:0033500 GO:0034284 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042044 GO:0042221 GO:0042592 GO:0042593 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044212 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0046031 GO:0046034 GO:0046365 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046872 GO:0046914 GO:0046939 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051156 GO:0051171 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051252 GO:0051716 GO:0055082 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080090 GO:0090332 GO:0090407 GO:0097159 GO:0098573 GO:0098588 GO:0098805 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901700 GO:1901701 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00052 ko00500 ko00520 ko00521 ko00524 ko01100 ko01110 ko01120 ko01130 ko01200 ko04066 ko04910 ko04930 ko04973 ko05230 map00010 map00051 map00052 map00500 map00520 map00521 map00524 map01100 map01110 map01120 map01130 map01200 map04066 map04910 map04930 map04973 map05230
Pfam Domains
Protein Families
Hexokinase_1 Hexokinase_2

Protein Analysis

498

Amino Acids

53.92

Weight (kDa)

6.05

Isoelectric Point (pI)

34.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hexokinase_1 PF00349 41 - 240 9.6e-72 Hexokinase
Hexokinase_2 PF03727 247 - 487 6.7e-83 Hexokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010710)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19860 AT2G19860 AT4G29130
fragaria_vesca FvH4_1g06270
malus_domestica MD02G1066500.v1.1 MD15G1197000.v1.1
prunus_persica Prupe.7G218800_v2.0.a1
pyrus_communis pycom02g05260 pycom15g17600
rosa_chinensis RchiOBHm_Chr2g0091971
rosa_laevigata RLG00000016267
rosa_multiflora Rmu_sc0009216.1_g000003 Rmu_sc0017125.1_g000004
rosa_roxburghii Rroxscaffold_2G00149570
rosa_rugosa Rorug02G0021900
rosa_samantha Rh2AG067600 Rh2BG066900 Rh2CG068200 Rh2DG066700
rosa_wichuraiana Rw2G005340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 983
Acc36I ACCTGC 1 cut(s) 160
Acc65I GGTACC 1 cut(s) 717
AccB1I GGYRCC 1 cut(s) 717
AccB7I CCANNNNNTGG 1 cut(s) 619
AccII CGCG 5 cut(s) 23, 38, 53, 786, 1350
AccIII TCCGGA 2 cut(s) 727, 1433
AclWI GGATC 3 cut(s) 1116, 1234, 1247
AcsI RAATTY 3 cut(s) 374, 458, 1343
AcuI CTGAAG 4 cut(s) 143, 465, 492, 1026
AcyI GRCGYC 2 cut(s) 76, 180
AfaI GTAC 3 cut(s) 336, 719, 1366
AfiI CCNNNNNNNGG 8 cut(s) 112, 271, 497, 619, 727, 827, 1215, 1236
AflII CTTAAG 1 cut(s) 1253
AgsI TTSAA 6 cut(s) 85, 235, 390, 599, 922, 997
AjiI CACGTC 1 cut(s) 48
AjnI CCWGG 5 cut(s) 496, 657, 925, 1242, 1452
Alw21I GWGCWC 4 cut(s) 92, 633, 1404, 1419
Alw26I GTCTC 1 cut(s) 1263
AlwI GGATC 3 cut(s) 1116, 1234, 1247
Ama87I CYCGRG 2 cut(s) 91, 105
Aor13HI TCCGGA 2 cut(s) 727, 1433
AoxI GGCC 3 cut(s) 102, 655, 1218
ApaI GGGCCC 2 cut(s) 106, 1222
ApeKI GCWGC 7 cut(s) 64, 225, 740, 1013, 1232, 1445, 1457
ApoI RAATTY 3 cut(s) 374, 458, 1343
ArsI GACNNNNNNTTYG 2 cut(s) 1415, 1447
Asp700I GAANNNNTTC 1 cut(s) 483
Asp718I GGTACC 1 cut(s) 717
AspLEI GCGC 6 cut(s) 23, 38, 53, 64, 788, 984
AspS9I GGNCC 7 cut(s) 102, 103, 564, 1125, 1218, 1219, 1431
AsuC2I CCSGG 4 cut(s) 106, 107, 205, 562
AsuHPI GGTGA 4 cut(s) 55, 625, 1037, 1397
AvaI CYCGRG 2 cut(s) 91, 105
AvaII GGWCC 3 cut(s) 564, 1125, 1431
BaeGI GKGCMC 3 cut(s) 106, 758, 1222
BamHI GGATCC 1 cut(s) 1239
BanI GGYRCC 1 cut(s) 717
BanII GRGCYC 4 cut(s) 92, 106, 633, 1222
BauI CACGAG 1 cut(s) 1212
BbsI GAAGAC 1 cut(s) 806
Bbv12I GWGCWC 4 cut(s) 92, 633, 1404, 1419
BbvI GCAGC 7 cut(s) 51, 237, 727, 1000, 1219, 1432, 1469
BccI CCATC 6 cut(s) 106, 829, 994, 1277, 1310, 1421
BcgI CGANNNNNNTGC 1 cut(s) 1473
BciT130I CCWGG 5 cut(s) 498, 659, 927, 1244, 1454
BclI TGATCA 1 cut(s) 895
BcnI CCSGG 4 cut(s) 106, 107, 205, 562
BcoDI GTCTC 1 cut(s) 1263
BfaI CTAG 2 cut(s) 452, 1145
BfoI RGCGCY 1 cut(s) 65
BfrI CTTAAG 1 cut(s) 1253
BfuAI ACCTGC 1 cut(s) 160
BglII AGATCT 1 cut(s) 1149
BlpI GCTNAGC 1 cut(s) 1398
BmcAI AGTACT 1 cut(s) 1366
Bme1390I CCNGG 9 cut(s) 106, 107, 205, 498, 562, 659, 927, 1244, 1454
Bme18I GGWCC 3 cut(s) 564, 1125, 1431
BmeT110I CYCGRG 2 cut(s) 91, 105
BmgBI CACGTC 1 cut(s) 48
BmgT120I GGNCC 7 cut(s) 102, 103, 564, 1125, 1218, 1219, 1431
BmiI GGNNCC 9 cut(s) 104, 565, 566, 719, 1126, 1219, 1220, 1241, 1432
BmrFI CCNGG 9 cut(s) 106, 107, 205, 498, 562, 659, 927, 1244, 1454
BmrI ACTGGG 2 cut(s) 279, 420
BmsI GCATC 6 cut(s) 186, 228, 592, 721, 898, 1099
BmuI ACTGGG 2 cut(s) 279, 420
BpiI GAAGAC 1 cut(s) 806
BplI GAGNNNNNCTC 4 cut(s) 1391, 1423, 1434, 1466
BpmI CTGGAG 2 cut(s) 729, 948
Bpu10I CCTNAGC 1 cut(s) 506
Bpu1102I GCTNAGC 1 cut(s) 1398
BpuEI CTTGAG 1 cut(s) 1118
BpuMI CCSGG 4 cut(s) 106, 107, 205, 562
BsaBI GATNNNNATC 2 cut(s) 242, 839
BsaHI GRCGYC 2 cut(s) 76, 180
BsaJI CCNNGG 6 cut(s) 105, 216, 304, 925, 1243, 1477
BsaWI WCCGGW 2 cut(s) 727, 1433
BsaXI ACNNNNNCTCC 4 cut(s) 822, 852, 963, 993
Bsc4I CCNNNNNNNGG 8 cut(s) 112, 271, 497, 619, 727, 827, 1215, 1236
Bse1I ACTGG 3 cut(s) 274, 415, 763
Bse3DI GCAATG 2 cut(s) 1092, 1317
Bse8I GATNNNNATC 2 cut(s) 242, 839
BseAI TCCGGA 2 cut(s) 727, 1433
BseBI CCWGG 5 cut(s) 498, 659, 927, 1244, 1454
BseDI CCNNGG 6 cut(s) 105, 216, 304, 925, 1243, 1477
BseGI GGATG 6 cut(s) 280, 487, 736, 966, 1288, 1321
BseJI GATNNNNATC 2 cut(s) 242, 839
BseLI CCNNNNNNNGG 8 cut(s) 112, 271, 497, 619, 727, 827, 1215, 1236
BseMI GCAATG 2 cut(s) 1092, 1317
BseMII CTCAG 2 cut(s) 903, 1389
BseNI ACTGG 3 cut(s) 274, 415, 763
BseRI GAGGAG 1 cut(s) 109
BseSI GKGCMC 3 cut(s) 106, 758, 1222
BseXI GCAGC 7 cut(s) 51, 237, 727, 1000, 1219, 1432, 1469
BseYI CCCAGC 2 cut(s) 514, 1235
BsgI GTGCAG 1 cut(s) 477
Bsh1236I CGCG 5 cut(s) 23, 38, 53, 786, 1350
BshFI GGCC 3 cut(s) 104, 657, 1220
BshNI GGYRCC 1 cut(s) 717
BsiHKAI GWGCWC 4 cut(s) 92, 633, 1404, 1419
BsiHKCI CYCGRG 2 cut(s) 91, 105
BsiSI CCGG 6 cut(s) 106, 166, 204, 561, 728, 1434
BslFI GGGAC 5 cut(s) 325, 427, 577, 712, 1138
BslI CCNNNNNNNGG 8 cut(s) 112, 271, 497, 619, 727, 827, 1215, 1236
BsmAI GTCTC 1 cut(s) 1263
BsmFI GGGAC 5 cut(s) 325, 427, 577, 712, 1138
BsnI GGCC 3 cut(s) 104, 657, 1220
BsoBI CYCGRG 2 cut(s) 91, 105
Bsp120I GGGCCC 2 cut(s) 102, 1218
Bsp1286I GDGCHC 7 cut(s) 92, 106, 633, 758, 1222, 1404, 1419
Bsp13I TCCGGA 2 cut(s) 727, 1433
Bsp1407I TGTACA 1 cut(s) 334
Bsp143I GATC 5 cut(s) 895, 931, 1108, 1149, 1239
Bsp1720I GCTNAGC 1 cut(s) 1398
BspANI GGCC 3 cut(s) 104, 657, 1220
BspCNI CTCAG 2 cut(s) 904, 1390
BspEI TCCGGA 2 cut(s) 727, 1433
BspFNI CGCG 5 cut(s) 23, 38, 53, 786, 1350
BspHI TCATGA 1 cut(s) 1093
BspLI GGNNCC 9 cut(s) 104, 565, 566, 719, 1126, 1219, 1220, 1241, 1432
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 3 cut(s) 1116, 1234, 1247
BspQI GCTCTTC 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 717
BspTI CTTAAG 1 cut(s) 1253
BsrDI GCAATG 2 cut(s) 1092, 1317
BsrGI TGTACA 1 cut(s) 334
BsrI ACTGG 3 cut(s) 274, 415, 763
BssECI CCNNGG 6 cut(s) 105, 216, 304, 925, 1243, 1477
BssMI GATC 5 cut(s) 895, 931, 1108, 1149, 1239
BssNI GRCGYC 2 cut(s) 76, 180
BssSI CACGAG 1 cut(s) 1212
BssT1I CCWWGG 2 cut(s) 304, 1477
Bst2BI CACGAG 1 cut(s) 1212
Bst2UI CCWGG 5 cut(s) 498, 659, 927, 1244, 1454
Bst4CI ACNGT 3 cut(s) 190, 1033, 1276
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 2 cut(s) 76, 180
BstAFI CTTAAG 1 cut(s) 1253
BstAUI TGTACA 1 cut(s) 334
BstC8I GCNNGC 3 cut(s) 201, 210, 1222
BstDEI CTNAG 3 cut(s) 506, 912, 1398
BstENI CCTNNNNNAGG 1 cut(s) 825
BstF5I GGATG 6 cut(s) 280, 487, 736, 966, 1288, 1321
BstFNI CGCG 5 cut(s) 23, 38, 53, 786, 1350
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 6 cut(s) 23, 38, 53, 64, 788, 984
BstKTI GATC 5 cut(s) 898, 934, 1111, 1152, 1242
BstMAI GTCTC 1 cut(s) 1263
BstMBI GATC 5 cut(s) 895, 931, 1108, 1149, 1239
BstNI CCWGG 5 cut(s) 498, 659, 927, 1244, 1454
BstNSI RCATGY 1 cut(s) 794
BstSCI CCNGG 9 cut(s) 104, 105, 203, 496, 560, 657, 925, 1242, 1452
BstSLI GKGCMC 3 cut(s) 106, 758, 1222
BstUI CGCG 5 cut(s) 23, 38, 53, 786, 1350
BstV1I GCAGC 7 cut(s) 51, 237, 727, 1000, 1219, 1432, 1469
BstV2I GAAGAC 1 cut(s) 806
BstX2I RGATCY 3 cut(s) 1108, 1149, 1239
BstXI CCANNNNNNTGG 3 cut(s) 190, 643, 1478
BstYI RGATCY 3 cut(s) 1108, 1149, 1239
BsuRI GGCC 3 cut(s) 104, 657, 1220
BtgZI GCGATG 1 cut(s) 125
BtrI CACGTC 1 cut(s) 48
BtsCI GGATG 6 cut(s) 280, 487, 736, 966, 1288, 1321
BtsIMutI CAGTG 6 cut(s) 267, 648, 756, 1163, 1308, 1338
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 3 cut(s) 201, 210, 1222
CciI TCATGA 1 cut(s) 1093
CfoI GCGC 6 cut(s) 23, 38, 53, 64, 788, 984
Cfr13I GGNCC 7 cut(s) 102, 103, 564, 1125, 1218, 1219, 1431
Cfr9I CCCGGG 1 cut(s) 105
CseI GACGC 4 cut(s) 65, 188, 201, 1339
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 3 cut(s) 335, 718, 1365
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CviAII CATG 6 cut(s) 184, 791, 809, 847, 1094, 1357
CviQI GTAC 3 cut(s) 335, 718, 1365
DdeI CTNAG 3 cut(s) 506, 912, 1398
DpnI GATC 5 cut(s) 897, 933, 1110, 1151, 1241
DpnII GATC 5 cut(s) 895, 931, 1108, 1149, 1239
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
EciI GGCGGA 1 cut(s) 641
Ecl136II GAGCTC 2 cut(s) 90, 631
Eco130I CCWWGG 2 cut(s) 304, 1477
Eco24I GRGCYC 4 cut(s) 92, 106, 633, 1222
Eco32I GATATC 1 cut(s) 1141
Eco47I GGWCC 3 cut(s) 564, 1125, 1431
Eco53kI GAGCTC 2 cut(s) 90, 631
Eco57I CTGAAG 4 cut(s) 143, 465, 492, 1026
Eco88I CYCGRG 2 cut(s) 91, 105
EcoICRI GAGCTC 2 cut(s) 90, 631
EcoNI CCTNNNNNAGG 1 cut(s) 825
EcoO109I RGGNCCY 2 cut(s) 564, 1218
EcoRI GAATTC 1 cut(s) 1343
EcoRII CCWGG 5 cut(s) 496, 657, 925, 1242, 1452
EcoRV GATATC 1 cut(s) 1141
EcoT14I CCWWGG 2 cut(s) 304, 1477
EcoT22I ATGCAT 2 cut(s) 298, 1092
EcoT38I GRGCYC 4 cut(s) 92, 106, 633, 1222
ErhI CCWWGG 2 cut(s) 304, 1477
FaeI CATG 6 cut(s) 187, 794, 812, 850, 1097, 1360
FaqI GGGAC 5 cut(s) 325, 427, 577, 712, 1138
FatI CATG 6 cut(s) 183, 790, 808, 846, 1093, 1356
FauI CCCGC 1 cut(s) 1229
FbaI TGATCA 1 cut(s) 895
FokI GGATG 6 cut(s) 287, 474, 743, 973, 1295, 1328
FriOI GRGCYC 4 cut(s) 92, 106, 633, 1222
FspBI CTAG 2 cut(s) 452, 1145
FspI TGCGCA 1 cut(s) 983
GlaI GCGC 6 cut(s) 22, 37, 52, 63, 787, 983
GsaI CCCAGC 2 cut(s) 518, 1239
GsuI CTGGAG 2 cut(s) 729, 948
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 3 cut(s) 104, 657, 1220
HapII CCGG 6 cut(s) 106, 166, 204, 561, 728, 1434
HgaI GACGC 4 cut(s) 65, 188, 201, 1339
HhaI GCGC 6 cut(s) 23, 38, 53, 64, 788, 984
Hin1I GRCGYC 2 cut(s) 76, 180
Hin1II CATG 6 cut(s) 187, 794, 812, 850, 1097, 1360
Hin6I GCGC 6 cut(s) 21, 36, 51, 62, 786, 982
HinP1I GCGC 6 cut(s) 21, 36, 51, 62, 786, 982
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HinfI GANTC 5 cut(s) 588, 1069, 1097, 1248, 1490
HpaII CCGG 6 cut(s) 106, 166, 204, 561, 728, 1434
HphI GGTGA 4 cut(s) 55, 625, 1037, 1397
Hpy166II GTNNAC 3 cut(s) 335, 579, 610
Hpy188I TCNGA 3 cut(s) 217, 421, 1113
Hpy188III TCNNGA 6 cut(s) 728, 828, 1073, 1094, 1106, 1434
Hpy8I GTNNAC 3 cut(s) 335, 579, 610
Hpy99I CGWCG 2 cut(s) 81, 182
HpyAV CCTTC 7 cut(s) 118, 343, 416, 467, 473, 535, 887
HpyCH4III ACNGT 3 cut(s) 190, 1033, 1276
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 5 cut(s) 199, 296, 494, 1090, 1356
HpyF3I CTNAG 3 cut(s) 506, 912, 1398
HpySE526I ACGT 1 cut(s) 47
Hsp92I GRCGYC 2 cut(s) 76, 180
Hsp92II CATG 6 cut(s) 187, 794, 812, 850, 1097, 1360
HspAI GCGC 6 cut(s) 21, 36, 51, 62, 786, 982
KflI GGGWCCC 1 cut(s) 564
Kpn2I TCCGGA 2 cut(s) 727, 1433
KpnI GGTACC 1 cut(s) 721
Ksp22I TGATCA 1 cut(s) 895
Kzo9I GATC 5 cut(s) 895, 931, 1108, 1149, 1239
LguI GCTCTTC 1 cut(s) 80
LmnI GCTCC 4 cut(s) 168, 511, 628, 1442
Lsp1109I GCAGC 7 cut(s) 51, 237, 727, 1000, 1219, 1432, 1469
LweI GCATC 6 cut(s) 186, 228, 592, 721, 898, 1099
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 2 cut(s) 452, 1145
MaeII ACGT 1 cut(s) 47
MaeIII GTNAC 5 cut(s) 43, 143, 670, 857, 1196
MalI GATC 5 cut(s) 897, 933, 1110, 1151, 1241
MboI GATC 5 cut(s) 895, 931, 1108, 1149, 1239
MboII GAAGA 7 cut(s) 97, 247, 611, 806, 958, 1009, 1019
MfeI CAATTG 2 cut(s) 552, 693
MflI RGATCY 3 cut(s) 1108, 1149, 1239
MhlI GDGCHC 7 cut(s) 92, 106, 633, 758, 1222, 1404, 1419
MluCI AATT 8 cut(s) 374, 390, 458, 552, 651, 693, 1343, 1437
MlyI GAGTC 3 cut(s) 1063, 1091, 1257
MnlI CCTC 8 cut(s) 87, 90, 127, 211, 272, 704, 1471, 1480
Mph1103I ATGCAT 2 cut(s) 298, 1092
MroI TCCGGA 2 cut(s) 727, 1433
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 4 cut(s) 366, 684, 1064, 1254
MslI CAYNNNNRTG 2 cut(s) 188, 1422
MspA1I CMGCKG 1 cut(s) 1232
MspCI CTTAAG 1 cut(s) 1253
MspI CCGG 6 cut(s) 106, 166, 204, 561, 728, 1434
MspR9I CCNGG 9 cut(s) 106, 107, 205, 498, 562, 659, 927, 1244, 1454
MunI CAATTG 2 cut(s) 552, 693
MvaI CCWGG 5 cut(s) 498, 659, 927, 1244, 1454
MvnI CGCG 5 cut(s) 23, 38, 53, 786, 1350
NciI CCSGG 4 cut(s) 106, 107, 205, 562
NdeII GATC 5 cut(s) 895, 931, 1108, 1149, 1239
NlaIII CATG 6 cut(s) 187, 794, 812, 850, 1097, 1360
NlaIV GGNNCC 9 cut(s) 104, 565, 566, 719, 1126, 1219, 1220, 1241, 1432
NmuCI GTSAC 3 cut(s) 43, 143, 1196
NsbI TGCGCA 1 cut(s) 983
NsiI ATGCAT 2 cut(s) 298, 1092
NspI RCATGY 1 cut(s) 794
PaeR7I CTCGAG 1 cut(s) 91
PagI TCATGA 1 cut(s) 1093
PciSI GCTCTTC 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 1 cut(s) 588
PflFI GACNNNGTC 1 cut(s) 1202
PflMI CCANNNNNTGG 1 cut(s) 619
PfoI TCCNGGA 1 cut(s) 560
PleI GAGTC 3 cut(s) 1063, 1091, 1256
PpsI GAGTC 3 cut(s) 1063, 1091, 1256
PpuMI RGGWCCY 1 cut(s) 564
Psp124BI GAGCTC 2 cut(s) 92, 633
Psp5II RGGWCCY 1 cut(s) 564
Psp6I CCWGG 5 cut(s) 496, 657, 925, 1242, 1452
PspFI CCCAGC 2 cut(s) 514, 1235
PspGI CCWGG 5 cut(s) 496, 657, 925, 1242, 1452
PspN4I GGNNCC 9 cut(s) 104, 565, 566, 719, 1126, 1219, 1220, 1241, 1432
PspOMI GGGCCC 2 cut(s) 102, 1218
PspPI GGNCC 7 cut(s) 102, 103, 564, 1125, 1218, 1219, 1431
PspPPI RGGWCCY 1 cut(s) 564
PspXI VCTCGAGB 1 cut(s) 91
PsrI GAACNNNNNNTAC 2 cut(s) 850, 882
PsuI RGATCY 3 cut(s) 1108, 1149, 1239
PsyI GACNNNGTC 1 cut(s) 1202
RsaI GTAC 3 cut(s) 336, 719, 1366
RsaNI GTAC 3 cut(s) 335, 718, 1365
RseI CAYNNNNRTG 2 cut(s) 188, 1422
SacI GAGCTC 2 cut(s) 92, 633
SapI GCTCTTC 1 cut(s) 80
SaqAI TTAA 4 cut(s) 366, 684, 1064, 1254
Sau3AI GATC 5 cut(s) 895, 931, 1108, 1149, 1239
Sau96I GGNCC 7 cut(s) 102, 103, 564, 1125, 1218, 1219, 1431
ScaI AGTACT 1 cut(s) 1366
SchI GAGTC 3 cut(s) 1063, 1091, 1257
ScrFI CCNGG 9 cut(s) 106, 107, 205, 498, 562, 659, 927, 1244, 1454
SduI GDGCHC 7 cut(s) 92, 106, 633, 758, 1222, 1404, 1419
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 6 cut(s) 186, 228, 592, 721, 898, 1099
Sfr274I CTCGAG 1 cut(s) 91
SinI GGWCC 3 cut(s) 564, 1125, 1431
SlaI CTCGAG 1 cut(s) 91
SmaI CCCGGG 1 cut(s) 107
SmiMI CAYNNNNRTG 2 cut(s) 188, 1422
SmlI CTYRAG 3 cut(s) 91, 1133, 1253
SmoI CTYRAG 3 cut(s) 91, 1133, 1253
SrfI GCCCGGGC 1 cut(s) 107
Sse9I AATT 8 cut(s) 374, 390, 458, 552, 651, 693, 1343, 1437
SspMI CTAG 2 cut(s) 452, 1145
SstI GAGCTC 2 cut(s) 92, 633
StyD4I CCNGG 9 cut(s) 104, 105, 203, 496, 560, 657, 925, 1242, 1452
StyI CCWWGG 2 cut(s) 304, 1477
TaaI ACNGT 3 cut(s) 190, 1033, 1276
TaiI ACGT 1 cut(s) 50
TaqI TCGA 1 cut(s) 92
TasI AATT 8 cut(s) 374, 390, 458, 552, 651, 693, 1343, 1437
TatI WGTACW 2 cut(s) 334, 1364
TauI GCSGC 8 cut(s) 26, 41, 56, 59, 62, 76, 225, 773
TfiI GAWTC 1 cut(s) 588
Tru1I TTAA 4 cut(s) 366, 684, 1064, 1254
Tru9I TTAA 4 cut(s) 366, 684, 1064, 1254
TscAI CASTG 6 cut(s) 274, 648, 763, 1170, 1308, 1345
TseFI GTSAC 3 cut(s) 43, 143, 1196
TseI GCWGC 7 cut(s) 64, 225, 740, 1013, 1232, 1445, 1457
Tsp45I GTSAC 3 cut(s) 43, 143, 1196
TspDTI ATGAA 6 cut(s) 396, 476, 587, 858, 1048, 1404
TspGWI ACGGA 1 cut(s) 311
TspMI CCCGGG 1 cut(s) 105
TspRI CASTG 6 cut(s) 274, 648, 763, 1170, 1308, 1345
Tth111I GACNNNGTC 1 cut(s) 1202
Van91I CCANNNNNTGG 1 cut(s) 619
Vha464I CTTAAG 1 cut(s) 1253
VpaK11BI GGWCC 3 cut(s) 564, 1125, 1431
XagI CCTNNNNNAGG 1 cut(s) 825
XapI RAATTY 3 cut(s) 374, 458, 1343
XceI RCATGY 1 cut(s) 794
XhoI CTCGAG 1 cut(s) 91
XmaI CCCGGG 1 cut(s) 105
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 2 cut(s) 452, 1145
ZrmI AGTACT 1 cut(s) 1366
Zsp2I ATGCAT 2 cut(s) 298, 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.