Rroxscaffold_2G00150030

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019380
Physical Location & Seq
Reverse (-)
87491613 .. 87492644
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00150030.1

Sequence Viewer

Length: 765 bp
ATGATTAATGGGTATGTCAAAACTTGTGTTTACTGTGAAGCTTTCAGGTTGTTCGTAGAAATGTTTCGTTGTTGTAACGCATTACTGGATGAGTATACATTTTCGACAATGGCAAAAGTTTCCAGCGAGGTTGGGGACTTGGTGGCCGGGAAATGGGTTTCATGGGAAGAGTGTGAGATTTGGGATGTTGGGAAATTGGGTTATGGGAGGGAGCTTCATTGTTATATTGTGAAGTATGGATTGGATTTGAATTTGGGTTTGGATGTTCATCTTGAGTGTTGTTTGGTTGATATGTATTGTAGGAGTGGTAGTGTTGATTTGGGGAGACGGATGTTTCAGCGCATGAAGCGTAGAAATGTATATGCTTGGACGATGATGGCCTATACTTATGTGCAGAATGGAGCGTCTGATGAAGAATTGATTCTCTTCTGGAGGATGCAGGTGGAAGATGGGATAGAACCCAATAGAGTATCACTTGTAAGTGTTCTCCCAGCTTGTATTTCAGATGCTGGTTTAACGTGTGGGAAACAAATTCATGGGTTCGCTATCAGGAAGGTGTTAAGGCGAGTCTTTGAGGATGACTCTTTTTGTAAGGATGCAATCTCTTGGAGTTCATTGATATCACGGTATGGATTATATGGAAGGGGTGAGGAAGCCATTGTTTTGTATAATAAGATGCTTCAGCTTGGAATCAAACCGGATATGACCATAGTAGGCCTTCTTTCAGCTTGTGGCAGGTCAGGATTGGTAAATGAAGACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

254

Amino Acids

28.93

Weight (kDa)

5.52

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 199 - 235 8.5e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019380)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0091491
rosa_laevigata RLG00000016228
rosa_multiflora Rmu_sc0002407.1_g000011
rosa_roxburghii Rroxscaffold_2G00150030
rosa_rugosa Rorug02G0018200
rosa_samantha Rh2AG063200 Rh2BG062800 Rh2CG064500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 430
Acc36I ACCTGC 2 cut(s) 430, 726
AccI GTMKAC 1 cut(s) 95
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 2 cut(s) 250, 531
AcuI CTGAAG 1 cut(s) 665
AfiI CCNNNNNNNGG 1 cut(s) 153
AflIII ACRYGT 1 cut(s) 518
AgsI TTSAA 1 cut(s) 250
AjuI GAANNNNNNNTTGG 4 cut(s) 224, 242, 256, 274
AluBI AGCT 5 cut(s) 41, 214, 494, 685, 728
AluI AGCT 5 cut(s) 41, 214, 494, 685, 728
Alw26I GTCTC 1 cut(s) 319
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 3 cut(s) 144, 378, 715
ApoI RAATTY 2 cut(s) 250, 531
AseI ATTAAT 1 cut(s) 6
Asp700I GAANNNNTTC 2 cut(s) 63, 420
AspLEI GCGC 1 cut(s) 342
AsuC2I CCSGG 1 cut(s) 148
AsuHPI GGTGA 1 cut(s) 659
BccI CCATC 2 cut(s) 370, 443
BcnI CCSGG 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 319
BfuAI ACCTGC 2 cut(s) 430, 726
Bme1390I CCNGG 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 148
BmsI GCATC 4 cut(s) 426, 496, 586, 666
BplI GAGNNNNNCTC 2 cut(s) 566, 598
BpmI CTGGAG 1 cut(s) 451
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 148
BsaBI GATNNNNATC 1 cut(s) 267
BsaWI WCCGGW 1 cut(s) 697
BsaXI ACNNNNNCTCC 2 cut(s) 295, 325
Bsc4I CCNNNNNNNGG 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 90
Bse8I GATNNNNATC 1 cut(s) 267
BseGI GGATG 7 cut(s) 94, 190, 268, 336, 441, 583, 601
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 90
BseYI CCCAGC 1 cut(s) 490
BsgI GTGCAG 1 cut(s) 413
BshFI GGCC 3 cut(s) 146, 380, 717
BsiSI CCGG 2 cut(s) 147, 698
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 153
BsmAI GTCTC 1 cut(s) 319
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 3 cut(s) 146, 380, 717
BspANI GGCC 3 cut(s) 146, 380, 717
BspMI ACCTGC 2 cut(s) 430, 726
BsrI ACTGG 1 cut(s) 90
BssNAI GTATAC 1 cut(s) 96
Bst1107I GTATAC 1 cut(s) 96
Bst4CI ACNGT 2 cut(s) 35, 627
Bst6I CTCTTC 2 cut(s) 162, 431
BstF5I GGATG 7 cut(s) 94, 190, 268, 336, 441, 583, 601
BstHHI GCGC 1 cut(s) 342
BstMAI GTCTC 1 cut(s) 319
BstMWI GCNNNNNNNGC 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 146
BstZ17I GTATAC 1 cut(s) 96
BsuRI GGCC 3 cut(s) 146, 380, 717
BtsCI GGATG 7 cut(s) 94, 190, 268, 336, 441, 583, 601
BveI ACCTGC 2 cut(s) 430, 726
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 1 cut(s) 342
CseI GACGC 1 cut(s) 393
CviAII CATG 3 cut(s) 162, 343, 536
CviJI RGCY 9 cut(s) 41, 146, 214, 380, 494, 656, 685, 717, 728
CviKI_1 RGCY 9 cut(s) 41, 146, 214, 380, 494, 656, 685, 717, 728
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 2 cut(s) 162, 431
EarI CTCTTC 2 cut(s) 162, 431
Eco147I AGGCCT 1 cut(s) 717
Eco32I GATATC 1 cut(s) 621
Eco57I CTGAAG 1 cut(s) 665
EcoRV GATATC 1 cut(s) 621
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 3 cut(s) 165, 346, 539
FaqI GGGAC 1 cut(s) 149
FatI CATG 3 cut(s) 161, 342, 535
FblI GTMKAC 1 cut(s) 95
FokI GGATG 7 cut(s) 101, 197, 275, 343, 448, 590, 608
GlaI GCGC 1 cut(s) 341
GsaI CCCAGC 1 cut(s) 494
GsuI CTGGAG 1 cut(s) 451
HaeIII GGCC 3 cut(s) 146, 380, 717
HapII CCGG 2 cut(s) 147, 698
HgaI GACGC 1 cut(s) 393
HhaI GCGC 1 cut(s) 342
Hin1II CATG 3 cut(s) 165, 346, 539
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 4 cut(s) 421, 567, 581, 690
HpaII CCGG 2 cut(s) 147, 698
HphI GGTGA 1 cut(s) 659
Hpy166II GTNNAC 2 cut(s) 31, 96
Hpy188I TCNGA 2 cut(s) 409, 505
Hpy188III TCNNGA 4 cut(s) 272, 430, 550, 741
Hpy8I GTNNAC 2 cut(s) 31, 96
HpyAV CCTTC 3 cut(s) 547, 636, 728
HpyCH4III ACNGT 2 cut(s) 35, 627
HpyCH4IV ACGT 1 cut(s) 518
HpyCH4V TGCA 3 cut(s) 394, 439, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 346
HpySE526I ACGT 1 cut(s) 518
Hsp92II CATG 3 cut(s) 165, 346, 539
HspAI GCGC 1 cut(s) 340
LmnI GCTCC 2 cut(s) 211, 401
LweI GCATC 4 cut(s) 426, 496, 586, 666
MaeII ACGT 1 cut(s) 518
MaeIII GTNAC 1 cut(s) 74
MboII GAAGA 4 cut(s) 179, 418, 425, 458
MluCI AATT 4 cut(s) 194, 250, 416, 531
MlyI GAGTC 2 cut(s) 575, 576
MnlI CCTC 5 cut(s) 121, 201, 426, 568, 643
MroXI GAANNNNTTC 2 cut(s) 63, 420
MseI TTAA 4 cut(s) 6, 515, 560, 763
MspI CCGG 2 cut(s) 147, 698
MspR9I CCNGG 1 cut(s) 148
MwoI GCNNNNNNNGC 1 cut(s) 346
NciI CCSGG 1 cut(s) 148
NlaIII CATG 3 cut(s) 165, 346, 539
PaqCI CACCTGC 1 cut(s) 430
PceI AGGCCT 1 cut(s) 717
PdmI GAANNNNTTC 2 cut(s) 63, 420
PfeI GAWTC 2 cut(s) 421, 690
PleI GAGTC 2 cut(s) 575, 575
PpsI GAGTC 2 cut(s) 575, 575
PshBI ATTAAT 1 cut(s) 6
PspFI CCCAGC 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 509
SaqAI TTAA 4 cut(s) 6, 515, 560, 763
SchI GAGTC 2 cut(s) 575, 576
ScrFI CCNGG 1 cut(s) 148
SfaNI GCATC 4 cut(s) 426, 496, 586, 666
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 4 cut(s) 194, 250, 416, 531
SseBI AGGCCT 1 cut(s) 717
StuI AGGCCT 1 cut(s) 717
StyD4I CCNGG 1 cut(s) 146
TaaI ACNGT 2 cut(s) 35, 627
TaiI ACGT 1 cut(s) 521
TaqI TCGA 1 cut(s) 104
TasI AATT 4 cut(s) 194, 250, 416, 531
TfiI GAWTC 2 cut(s) 421, 690
Tru1I TTAA 4 cut(s) 6, 515, 560, 763
Tru9I TTAA 4 cut(s) 6, 515, 560, 763
TspDTI ATGAA 7 cut(s) 150, 206, 257, 359, 426, 524, 603
TspGWI ACGGA 1 cut(s) 343
VspI ATTAAT 1 cut(s) 6
XapI RAATTY 2 cut(s) 250, 531
XmiI GTMKAC 1 cut(s) 95
XmnI GAANNNNTTC 2 cut(s) 63, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.