Rroxscaffold_3G00246060

methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002835
Physical Location & Seq
Forward (+)
39157971 .. 39162694
4724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00246060.1

Sequence Viewer

Length: 1803 bp
ATGAAGTATAATAAAGAAGCAAAACCGGGTGCGCAGGAGAAAAGCTCCAGGGTTTTACAGTTGGCAGTACCGTTTTTCTTGCTGTGCGGGTTTTCATTCTATCTTGGTGGAATCTTCTGCTCTGAGAAGACCAGATTGGAGACTAATGGTGTCAAGATCGCAGTTCCTTCTGCTAAGGAGTCACTGGCGGCCCCTCTCCAGATAAAAGCTGTGTCCTTCCCTGAATGCAGTGCCGACTATCAAGACTACACTCCTTGCACAGATCCAAGGAGGTGGAGGAAATATGGTGTTCATCGGCTTACCTTCATGGAACGCCACTGCCCTCCAGTATTTGAAAGGAAAGAATGCCTAGTTCCACCTCCAGATGGATACAAGTCTCCAATCAAGTGGCCAAATAGCCGGGATAAATGTTGGTACAGGAATGTGCCATATGATTGGATTAACAAGCAGAAATCTAATCAGAATTGGCTGAGAAAAGAAGGAGACAAGTTTCTTTTTCCTGGTGGGGGAACTATGTTTCCTAAAGGTGTTTCTGCATATGTTGATTTAATGCAAGATCTTATTCCAGAAATGAAAGATGGCACTATCCGAACTGCCATCGACACTGGTTGTGGGGTTGCAAGTTGGGGAGGTGATTTGTTAGATCGTGGGATTTTAACTGTATCTCTTGCACCAAGAGATAATCATGAAGCTCAAGTTCAGTTCGCATTGGAACGTGGAATTCCAGCAGTCCTTGGCATCATTTCCACTCAGAGGCTTCCTTTCCCCTCAAAGTCATTTGATATGGCTCACTGTTCTAGATGCCTTATCCCATGGACAGAATTCGGTGGAATTTACCTGCTAGAAGTTCATCGCATACTTCGTCCTGGAGGTTTCTGGGTCCTCTCTGGTCCACCTGTCAACTACGAAAACCGCTGGCGTGGATGGAACACAACTATAGAAGACCAGAAAACAGATTACGAGAAGTTGCAAGATCTACTAACTTCATTGTGCTTCAAATTGTACAACAAAAAGGATGACATTGCTGTTTGGCAGAAGTTGTCAGACAATAGTTGCTATGATAAGCTCTCTGAACCAGATACCTATCCTGCAAAGTGTGATGATAGTCTTGAACCAGATTCAGCATGGTACACTCCATTGCGCTCTTGTGTTGTCGTTCCAAACCCAAAGCACAAAAGGTCGTCTTTGGACACCATCCCTAAATGGCCAGAGCGGTTGCATGTTGCACCAGAACGTATTTCTGATGTTCATGGTGGGAGTGCTAGTGCTCTGAAGCATGATGATGGCAAGTGGAAGAAACGATTGCAGCACTACAAGAAGTTGCTCCCAGCAGTGGGAACAGATAAGATCAGAAATGTTATGGACATGAATACCGTGTATGGAGGGCTTGCAGCAGCCTCAATTGATGATCCCTTGTGGGTCATGAATGTGGTTTCTTCCTACGCTGCAAATACACTGCCTGTGGTCTATGATCGGGGCCTTATTGGAACATACCATGACTGGTGCGAAGCTTTCTCTACATATCCCCGAACTTATGATCTTCTACATCTTGATGGCCTCTTTACTGCAGAAAGCCACAGATGCGAAATGAAATACATGCTCCTAGAGATGGACCGCATCCTGCGCCCCAATGGCTACGCAATAATTCGGGAATCCAGCTACTTTGTGGATGCTGTCGCAACTATTGCCAAAGGGATGAGATGGGGATGTCGCAAAGAAGATACTGAGTATGGTGTTGAGAAGGAGAAAATATTGATCTGCCAGAAAAAGCTTTGGTATTCATCTAACCAGAGTTCTAGATGA

Protein Analysis

600

Amino Acids

68.5

Weight (kDa)

8.61

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 82 - 589 1.2e-215 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 33
Acc36I ACCTGC 1 cut(s) 846
AccBSI CCGCTC 1 cut(s) 1213
AciI CCGC 5 cut(s) 87, 188, 913, 1213, 1615
AclWI GGATC 2 cut(s) 257, 1403
AcoI YGGCCR 2 cut(s) 389, 1205
AcsI RAATTY 3 cut(s) 720, 821, 831
AcuI CTGAAG 1 cut(s) 1292
AfaI GTAC 4 cut(s) 69, 416, 1004, 1130
AfiI CCNNNNNNNGG 6 cut(s) 365, 506, 753, 1333, 1334, 1609
AgsI TTSAA 3 cut(s) 335, 997, 1112
AjnI CCWGG 3 cut(s) 47, 499, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AloI GAACNNNNNNTCC 2 cut(s) 502, 534
AluBI AGCT 7 cut(s) 45, 209, 692, 1066, 1511, 1659, 1771
AluI AGCT 7 cut(s) 45, 209, 692, 1066, 1511, 1659, 1771
Alw21I GWGCWC 1 cut(s) 1270
Alw26I GTCTC 3 cut(s) 134, 381, 477
AlwI GGATC 2 cut(s) 257, 1403
AoxI GGCC 5 cut(s) 189, 389, 1205, 1477, 1555
ApeKI GCWGC 4 cut(s) 1306, 1391, 1394, 1445
ApoI RAATTY 3 cut(s) 720, 821, 831
AspLEI GCGC 3 cut(s) 34, 1143, 1626
AspS9I GGNCC 5 cut(s) 190, 880, 890, 1477, 1612
AsuC2I CCSGG 2 cut(s) 27, 401
AsuHPI GGTGA 1 cut(s) 644
AvaII GGWCC 3 cut(s) 880, 890, 1612
BalI TGGCCA 2 cut(s) 391, 1207
BbsI GAAGAC 2 cut(s) 134, 948
Bbv12I GWGCWC 1 cut(s) 1270
BbvI GCAGC 4 cut(s) 1318, 1403, 1406, 1432
BccI CCATC 9 cut(s) 359, 572, 605, 918, 1202, 1277, 1547, 1603, 1695
BciT130I CCWGG 3 cut(s) 49, 501, 867
BciVI GTATCC 1 cut(s) 362
BcnI CCSGG 2 cut(s) 27, 401
BcoDI GTCTC 3 cut(s) 134, 381, 477
BfaI CTAG 6 cut(s) 350, 798, 842, 1263, 1604, 1797
BfmI CTRYAG 2 cut(s) 936, 1566
BfuAI ACCTGC 1 cut(s) 846
BfuI GTATCC 1 cut(s) 362
BglI GCCNNNNNGGC 1 cut(s) 1632
BglII AGATCT 2 cut(s) 556, 973
BisI GCNGC 5 cut(s) 189, 1307, 1392, 1395, 1446
BlsI GCNGC 5 cut(s) 190, 1308, 1393, 1396, 1447
Bme1390I CCNGG 5 cut(s) 27, 49, 401, 501, 867
Bme18I GGWCC 3 cut(s) 880, 890, 1612
BmgT120I GGNCC 5 cut(s) 190, 880, 890, 1477, 1612
BmiI GGNNCC 3 cut(s) 192, 881, 1478
BmrFI CCNGG 5 cut(s) 27, 49, 401, 501, 867
BmsI GCATC 5 cut(s) 747, 791, 1571, 1626, 1660
BpiI GAAGAC 2 cut(s) 134, 948
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BpmI CTGGAG 5 cut(s) 31, 182, 309, 345, 888
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 678
BpuMI CCSGG 2 cut(s) 27, 401
BsaBI GATNNNNATC 1 cut(s) 1083
BsaJI CCNNGG 4 cut(s) 48, 266, 733, 812
BsaXI ACNNNNNCTCC 2 cut(s) 1249, 1279
Bsc4I CCNNNNNNNGG 6 cut(s) 365, 506, 753, 1333, 1334, 1609
Bse1I ACTGG 4 cut(s) 189, 326, 610, 1505
Bse3DI GCAATG 2 cut(s) 1020, 1136
Bse8I GATNNNNATC 1 cut(s) 1083
BseBI CCWGG 3 cut(s) 49, 501, 867
BseDI CCNNGG 4 cut(s) 48, 266, 733, 812
BseGI GGATG 7 cut(s) 929, 1021, 1194, 1617, 1675, 1701, 1712
BseJI GATNNNNATC 1 cut(s) 1083
BseLI CCNNNNNNNGG 6 cut(s) 365, 506, 753, 1333, 1334, 1609
BseMI GCAATG 2 cut(s) 1020, 1136
BseMII CTCAG 4 cut(s) 114, 461, 764, 1716
BseNI ACTGG 4 cut(s) 189, 326, 610, 1505
BseXI GCAGC 4 cut(s) 1318, 1403, 1406, 1432
BseYI CCCAGC 1 cut(s) 1327
BshFI GGCC 5 cut(s) 191, 391, 1207, 1479, 1557
BsiHKAI GWGCWC 1 cut(s) 1270
BsiSI CCGG 2 cut(s) 26, 400
BslI CCNNNNNNNGG 6 cut(s) 365, 506, 753, 1333, 1334, 1609
BsmAI GTCTC 3 cut(s) 134, 381, 477
BsmI GAATGC 2 cut(s) 230, 350
BsnI GGCC 5 cut(s) 191, 391, 1207, 1479, 1557
Bsp1286I GDGCHC 1 cut(s) 1270
Bsp1407I TGTACA 1 cut(s) 1002
Bsp19I CCATGG 1 cut(s) 812
BspACI CCGC 5 cut(s) 87, 188, 913, 1213, 1615
BspANI GGCC 5 cut(s) 191, 391, 1207, 1479, 1557
BspCNI CTCAG 4 cut(s) 115, 462, 763, 1717
BspHI TCATGA 2 cut(s) 685, 1422
BspLI GGNNCC 3 cut(s) 192, 881, 1478
BspMAI CTGCAG 1 cut(s) 1570
BspMI ACCTGC 1 cut(s) 846
BspPI GGATC 2 cut(s) 257, 1403
BsrBI CCGCTC 1 cut(s) 1213
BsrDI GCAATG 2 cut(s) 1020, 1136
BsrGI TGTACA 1 cut(s) 1002
BsrI ACTGG 4 cut(s) 189, 326, 610, 1505
BssECI CCNNGG 4 cut(s) 48, 266, 733, 812
BssT1I CCWWGG 3 cut(s) 266, 733, 812
Bst2UI CCWGG 3 cut(s) 49, 501, 867
Bst4CI ACNGT 5 cut(s) 60, 72, 661, 794, 1375
BstAPI GCANNNNNTGC 1 cut(s) 1685
BstAUI TGTACA 1 cut(s) 1002
BstC8I GCNNGC 2 cut(s) 917, 1389
BstDEI CTNAG 5 cut(s) 123, 174, 470, 750, 1725
BstDSI CCRYGG 1 cut(s) 812
BstF5I GGATG 7 cut(s) 929, 1021, 1194, 1617, 1675, 1701, 1712
BstHHI GCGC 3 cut(s) 34, 1143, 1626
BstMAI GTCTC 3 cut(s) 134, 381, 477
BstMWI GCNNNNNNNGC 4 cut(s) 1581, 1623, 1632, 1685
BstNI CCWGG 3 cut(s) 49, 501, 867
BstNSI RCATGY 2 cut(s) 1223, 1600
BstSCI CCNGG 5 cut(s) 25, 47, 399, 499, 865
BstSFI CTRYAG 2 cut(s) 936, 1566
BstV1I GCAGC 4 cut(s) 1318, 1403, 1406, 1432
BstV2I GAAGAC 2 cut(s) 134, 948
BstX2I RGATCY 3 cut(s) 262, 556, 973
BstXI CCANNNNNNTGG 3 cut(s) 273, 387, 435
BstYI RGATCY 3 cut(s) 262, 556, 973
BsuI GTATCC 1 cut(s) 362
BsuRI GGCC 5 cut(s) 191, 391, 1207, 1479, 1557
BtgI CCRYGG 1 cut(s) 812
BtgZI GCGATG 1 cut(s) 836
BtsCI GGATG 7 cut(s) 929, 1021, 1194, 1617, 1675, 1701, 1712
BtsI GCAGTG 4 cut(s) 235, 316, 1338, 1454
BtsIMutI CAGTG 7 cut(s) 182, 235, 316, 603, 790, 1338, 1454
BveI ACCTGC 1 cut(s) 846
Cac8I GCNNGC 2 cut(s) 917, 1389
CciI TCATGA 2 cut(s) 685, 1422
CfoI GCGC 3 cut(s) 34, 1143, 1626
Cfr13I GGNCC 5 cut(s) 190, 880, 890, 1477, 1612
Csp6I GTAC 4 cut(s) 68, 415, 1003, 1129
CviQI GTAC 4 cut(s) 68, 415, 1003, 1129
DdeI CTNAG 5 cut(s) 123, 174, 470, 750, 1725
EaeI YGGCCR 2 cut(s) 389, 1205
Eco130I CCWWGG 3 cut(s) 266, 733, 812
Eco47I GGWCC 3 cut(s) 880, 890, 1612
Eco57I CTGAAG 1 cut(s) 1292
EcoO109I RGGNCCY 2 cut(s) 880, 1477
EcoRI GAATTC 2 cut(s) 720, 821
EcoRII CCWGG 3 cut(s) 47, 499, 865
EcoT14I CCWWGG 3 cut(s) 266, 733, 812
ErhI CCWWGG 3 cut(s) 266, 733, 812
FalI AAGNNNNNCTT 2 cut(s) 1168, 1200
FauI CCCGC 1 cut(s) 80
FauNDI CATATG 2 cut(s) 430, 538
Fnu4HI GCNGC 5 cut(s) 189, 1307, 1392, 1395, 1446
FokI GGATG 7 cut(s) 936, 1028, 1181, 1604, 1682, 1708, 1719
Fsp4HI GCNGC 5 cut(s) 189, 1307, 1392, 1395, 1446
FspBI CTAG 6 cut(s) 350, 798, 842, 1263, 1604, 1797
FspI TGCGCA 1 cut(s) 33
GlaI GCGC 3 cut(s) 33, 1142, 1625
GluI GCNGC 5 cut(s) 189, 1307, 1392, 1395, 1446
GsaI CCCAGC 1 cut(s) 1331
GsuI CTGGAG 5 cut(s) 31, 182, 309, 345, 888
HaeIII GGCC 5 cut(s) 191, 391, 1207, 1479, 1557
HapII CCGG 2 cut(s) 26, 400
HhaI GCGC 3 cut(s) 34, 1143, 1626
Hin6I GCGC 3 cut(s) 32, 1141, 1624
HinP1I GCGC 3 cut(s) 32, 1141, 1624
HincII GTYRAC 1 cut(s) 901
HindII GTYRAC 1 cut(s) 901
HindIII AAGCTT 2 cut(s) 1509, 1769
HinfI GANTC 4 cut(s) 111, 179, 1118, 1652
HpaII CCGG 2 cut(s) 26, 400
HphI GGTGA 1 cut(s) 644
Hpy166II GTNNAC 3 cut(s) 893, 901, 1131
Hpy188I TCNGA 9 cut(s) 124, 462, 590, 753, 1045, 1072, 1243, 1272, 1352
Hpy8I GTNNAC 3 cut(s) 893, 901, 1131
HpyAV CCTTC 5 cut(s) 177, 226, 313, 473, 1735
HpyCH4III ACNGT 5 cut(s) 60, 72, 661, 794, 1375
HpyCH4IV ACGT 2 cut(s) 715, 1234
HpyF10VI GCNNNNNNNGC 4 cut(s) 1581, 1623, 1632, 1685
HpyF3I CTNAG 5 cut(s) 123, 174, 470, 750, 1725
HpySE526I ACGT 2 cut(s) 715, 1234
HspAI GCGC 3 cut(s) 32, 1141, 1624
LmnI GCTCC 3 cut(s) 50, 1329, 1605
Lsp1109I GCAGC 4 cut(s) 1318, 1403, 1406, 1432
LweI GCATC 5 cut(s) 747, 791, 1571, 1626, 1660
MaeI CTAG 6 cut(s) 350, 798, 842, 1263, 1604, 1797
MaeII ACGT 2 cut(s) 715, 1234
MaeIII GTNAC 1 cut(s) 180
MbiI CCGCTC 1 cut(s) 1213
MboII GAAGA 7 cut(s) 106, 139, 953, 1306, 1428, 1532, 1730
MfeI CAATTG 1 cut(s) 1401
MflI RGATCY 3 cut(s) 262, 556, 973
MhlI GDGCHC 1 cut(s) 1270
MlsI TGGCCA 2 cut(s) 391, 1207
MluCI AATT 7 cut(s) 463, 720, 821, 831, 998, 1401, 1644
MluNI TGGCCA 2 cut(s) 391, 1207
MlyI GAGTC 1 cut(s) 188
Mox20I TGGCCA 2 cut(s) 391, 1207
MscI TGGCCA 2 cut(s) 391, 1207
MseI TTAA 3 cut(s) 441, 548, 656
MslI CAYNNNNRTG 3 cut(s) 1281, 1427, 1551
Msp20I TGGCCA 2 cut(s) 391, 1207
MspA1I CMGCKG 1 cut(s) 915
MspI CCGG 2 cut(s) 26, 400
MspR9I CCNGG 5 cut(s) 27, 49, 401, 501, 867
MunI CAATTG 1 cut(s) 1401
Mva1269I GAATGC 2 cut(s) 230, 350
MvaI CCWGG 3 cut(s) 49, 501, 867
MwoI GCNNNNNNNGC 4 cut(s) 1581, 1623, 1632, 1685
NciI CCSGG 2 cut(s) 27, 401
NcoI CCATGG 1 cut(s) 812
NdeI CATATG 2 cut(s) 430, 538
NlaIV GGNNCC 3 cut(s) 192, 881, 1478
NmuCI GTSAC 1 cut(s) 180
NsbI TGCGCA 1 cut(s) 33
NspI RCATGY 2 cut(s) 1223, 1600
PagI TCATGA 2 cut(s) 685, 1422
PcsI WCGNNNNNNNCGW 1 cut(s) 859
PctI GAATGC 2 cut(s) 230, 350
PfeI GAWTC 3 cut(s) 111, 1118, 1652
PfoI TCCNGGA 1 cut(s) 865
PkrI GCNGC 5 cut(s) 190, 1308, 1393, 1396, 1447
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
PpuMI RGGWCCY 1 cut(s) 880
Psp5II RGGWCCY 1 cut(s) 880
Psp6I CCWGG 3 cut(s) 47, 499, 865
PspFI CCCAGC 1 cut(s) 1327
PspGI CCWGG 3 cut(s) 47, 499, 865
PspN4I GGNNCC 3 cut(s) 192, 881, 1478
PspPI GGNCC 5 cut(s) 190, 880, 890, 1477, 1612
PspPPI RGGWCCY 1 cut(s) 880
PstI CTGCAG 1 cut(s) 1570
PsuI RGATCY 3 cut(s) 262, 556, 973
RsaI GTAC 4 cut(s) 69, 416, 1004, 1130
RsaNI GTAC 4 cut(s) 68, 415, 1003, 1129
RseI CAYNNNNRTG 3 cut(s) 1281, 1427, 1551
SaqAI TTAA 3 cut(s) 441, 548, 656
SatI GCNGC 5 cut(s) 189, 1307, 1392, 1395, 1446
Sau96I GGNCC 5 cut(s) 190, 880, 890, 1477, 1612
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 5 cut(s) 27, 49, 401, 501, 867
SduI GDGCHC 1 cut(s) 1270
SfaNI GCATC 5 cut(s) 747, 791, 1571, 1626, 1660
SfcI CTRYAG 2 cut(s) 936, 1566
SinI GGWCC 3 cut(s) 880, 890, 1612
SmiMI CAYNNNNRTG 3 cut(s) 1281, 1427, 1551
SmlI CTYRAG 1 cut(s) 693
SmoI CTYRAG 1 cut(s) 693
Sse9I AATT 7 cut(s) 463, 720, 821, 831, 998, 1401, 1644
SsiI CCGC 5 cut(s) 87, 188, 913, 1213, 1615
SspI AATATT 1 cut(s) 1752
SspMI CTAG 6 cut(s) 350, 798, 842, 1263, 1604, 1797
StyD4I CCNGG 5 cut(s) 25, 47, 399, 499, 865
StyI CCWWGG 3 cut(s) 266, 733, 812
TaaI ACNGT 5 cut(s) 60, 72, 661, 794, 1375
TaiI ACGT 2 cut(s) 718, 1237
TaqI TCGA 1 cut(s) 600
TasI AATT 7 cut(s) 463, 720, 821, 831, 998, 1401, 1644
TatI WGTACW 1 cut(s) 1002
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 3 cut(s) 111, 1118, 1652
Tru1I TTAA 3 cut(s) 441, 548, 656
Tru9I TTAA 3 cut(s) 441, 548, 656
TscAI CASTG 7 cut(s) 189, 235, 323, 610, 797, 1338, 1461
TseFI GTSAC 1 cut(s) 180
TseI GCWGC 4 cut(s) 1306, 1391, 1394, 1445
Tsp45I GTSAC 1 cut(s) 180
TspRI CASTG 7 cut(s) 189, 235, 323, 610, 797, 1338, 1461
VpaK11BI GGWCC 3 cut(s) 880, 890, 1612
XapI RAATTY 3 cut(s) 720, 821, 831
XbaI TCTAGA 2 cut(s) 797, 1796
XceI RCATGY 2 cut(s) 1223, 1600
XcmI CCANNNNNNNNNTGG 2 cut(s) 1122, 1663
XspI CTAG 6 cut(s) 350, 798, 842, 1263, 1604, 1797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.