Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0013296

Physical Location & Seq

Genomic Coordinates Forward (+)

2835925 .. 2837747

Gene Structure 1823 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00280220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGGAAGACACACCAACCCTACCTCCATCCAAAACCTATGAACTAACAGCCTCCTTAATCTCATACACCAAATCCACCACCGCAACCACCACCTTTGCTCCACTTAACTTCCTCTTCAAGCCATGCACCTCCCTTTCTTCCCCCACCTACATCCTCCGCGATGTCACCCTTACCGCCTACCCCTCCCAAATCCTTGCCGTTGTTGGCCCTAGCGGGGCCGGAAAGTCCACCCTCCTAGACATCCTAGCCGCCCGAACCTCCCCCACTAGCGTTTTCGAGACCTTCTCCTTCGCCGCGTGCCTTCTCATCCCCAGCCCTAACAAGTCCAACATTGCCACCATCGTCTCCTCTCTTCTCTCCGAGCTCCGGCTGACTCACTTGACCAACACGCGGCTCGGCCACGGCCTATCCGGCGGTGAACGTCGCCGCGTTTCCATCGGCCTCAGCCTTCTCCATGACCCAGCGGTCCTACTTCTTGACGAGCCCACCTCCGGCCTCGACAGCACCTCCGCTTTCAACGTCATGCAGACCCTCAAGTCCATCTGCACCTCCCGTCACCGCACGGTGGTTCTATCAATCCACCAACCTAGCTTCAAGATCCTGTCCACCATTGATAGGATCCTGTTGCTGTCGAAAGGTGAAGTCGTCCATCACGGAACGCTTTCTTCTCTGGAAGAGTTTCTCCTCTCAAATGGCTTCACTGTCCCTCCACAGCTCAACGCCTTGGAATACACCATGGAAATTCTAAACCGCCTCACAGTCCCTAAACCTATCCCATCCACCACTATACCTCCATTAATTTCTTCTCCAAATTTTGATGATGGTGATGGAGCTCGACAAGTGGTCCGTTACAAAAGCTCTAGAATCCACGAGATTTTGACGCTTTACAATCGCTTTTGGAAAATCATATACAGAACTAGGCAGCTACTCTTAACAAACACGTTGGAGGCCTTACTTGTGGGTTTGGTTTTGGGCACCATTTACATAAACATCGGGTTTGACAAGCAAGGAATCGAGAAGAGGTTCGGTCTTTTCGCTTTCACTCTCACTTTCCTTCTTTCCTCCACAACCGAAACACTTCCAATCTTCATCAACGAAAGACCTATTGTTCTTAGAGAAACTTCCGGTGGGATTTACAGGCTCTCGTCTTATCTGATCGCCAATACCCTAGTTTTCTTGCCCTACTTGCTTGCGATTGCGATCATATACTCGGTTTCTGTCTACTTCTTGGTGGGTCTTTGCGCTAGTTGGCAAGCTTTCGCTTACTTTGTGCTGGTGATATGGATCATTGTGTTGATGGCCAACTCGTTTGTTTTGTTCTTAAGTTCCCTTGCTCCGAACTACATTGCCGGGACTTCGCTGGTGACGATTAGTCTCGGTGGTTTCTTTCTGTTTTCCGGCTACTTTATTTCTAAAGATAATATGCCCAAGTATTGGTTGTTTATGCATTTCTTTAGTATGTACAAGTATGCTCTTGATGCGCTTTTGATCAATGAGTACGGCTGCCTTGTGTCAAGGTGTATGATATGGTACTCCGATGATCGGAGTAAGAATAGTACAGAGAGTAGTACATGCATGGTGACCGGAGGCGATGTGCTGCAGAAGAGAGGGCTCCATGAAGGGCAGAGGTGGACCAACATTTACATCTTGATTGGATTCTTCGTTGCCTACCGTGTGCTATGTTTGATGGTTTTGGTCAGAAGGGTTTCAAGATCAAAGAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031224 GO:0042623 GO:0042626 GO:0043492 GO:0044425 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane ABC_tran

Protein Analysis

574

Amino Acids

63.94

Weight (kDa)

9.26

Isoelectric Point (pI)

42.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_tran	PF00005	52 - 90	5e-08	ABC transporter
ABC_tran	PF00005	115 - 164	7.5e-09	ABC transporter
ABC2_membrane_7	PF19055	194 - 256	7e-07	ABC-2 type transporter
ABC2_membrane	PF01061	291 - 500	3.9e-48	ABC-2 type transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013296)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52860
fragaria_vesca	FvH4_2g03830 FvH4_2g03830
malus_domestica	MD01G1210800.v1.1
prunus_persica	Prupe.2G303100_v2.0.a1
pyrus_communis	pycom01g22130 pycom07g25560
rosa_chinensis	RchiOBHm_Chr1g0378951
rosa_laevigata	RLG00000026406
rosa_multiflora	Rmu_sc0006761.1_g000007
rosa_roxburghii	Rroxscaffold_4G00280220 Rroxscaffold_4G00280250
rosa_rugosa	Rorug01G0411600
rosa_samantha	Rh1AG434200 Rh1BG391900 Rh1CG404700 Rh1DG422900
rosa_wichuraiana	Rw1G037900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	464, 535
AccB1I	GGYRCC	1 cut(s)	974
AccB7I	CCANNNNNTGG	1 cut(s)	1434
AccI	GTMKAC	1 cut(s)	1221
AccII	CGCG	4 cut(s)	159, 296, 391, 429
AclWI	GGATC	4 cut(s)	592, 613, 626, 1292
AcoI	YGGCCR	2 cut(s)	397, 1299
AcsI	RAATTY	2 cut(s)	741, 811
AfaI	GTAC	5 cut(s)	1463, 1499, 1532, 1558, 1570
AfiI	CCNNNNNNNGG	8 cut(s)	214, 366, 390, 491, 565, 615, 1434, 1542
AflII	CTTAAG	1 cut(s)	1321
AflIII	ACRYGT	1 cut(s)	939
AgsI	TTSAA	4 cut(s)	118, 517, 595, 1710
AhdI	GACNNNNNGTC	2 cut(s)	842, 1371
AluBI	AGCT	7 cut(s)	364, 591, 715, 833, 858, 925, 1256
AluI	AGCT	7 cut(s)	364, 591, 715, 833, 858, 925, 1256
Alw21I	GWGCWC	2 cut(s)	366, 835
Alw26I	GTCTC	3 cut(s)	272, 349, 1379
AlwI	GGATC	4 cut(s)	592, 613, 626, 1292
AoxI	GGCC	8 cut(s)	205, 216, 397, 403, 439, 493, 948, 1299
ApeKI	GCWGC	3 cut(s)	922, 1503, 1597
ApoI	RAATTY	2 cut(s)	741, 811
AseI	ATTAAT	1 cut(s)	797
Asp700I	GAANNNNTTC	5 cut(s)	661, 678, 1022, 1077, 1705
AspLEI	GCGC	2 cut(s)	1244, 1483
AspS9I	GGNCC	5 cut(s)	206, 216, 466, 844, 1632
AsuC2I	CCSGG	1 cut(s)	1351
AsuHPI	GGTGA	8 cut(s)	157, 428, 548, 650, 836, 1288, 1375, 1591
AvaII	GGWCC	3 cut(s)	466, 844, 1632
BaeGI	GKGCMC	1 cut(s)	977
BalI	TGGCCA	1 cut(s)	1301
BamHI	GGATCC	1 cut(s)	618
BanI	GGYRCC	1 cut(s)	974
BanII	GRGCYC	4 cut(s)	366, 486, 835, 1614
BauI	CACGAG	1 cut(s)	869
BbsI	GAAGAC	1 cut(s)	12
Bbv12I	GWGCWC	2 cut(s)	366, 835
BbvCI	CCTCAGC	1 cut(s)	443
BbvI	GCAGC	3 cut(s)	934, 1490, 1584
BceAI	ACGGC	3 cut(s)	182, 418, 1516
BclI	TGATCA	1 cut(s)	1488
BcnI	CCSGG	1 cut(s)	1351
BcoDI	GTCTC	3 cut(s)	272, 349, 1379
BfaI	CTAG	9 cut(s)	210, 236, 245, 267, 588, 861, 918, 1169, 1245
BfmI	CTRYAG	1 cut(s)	1598
BfrI	CTTAAG	1 cut(s)	1321
BglI	GCCNNNNNGGC	1 cut(s)	411
BisI	GCNGC	7 cut(s)	249, 294, 392, 427, 923, 1504, 1598
BlsI	GCNGC	7 cut(s)	250, 295, 393, 428, 924, 1505, 1599
Bme1390I	CCNGG	1 cut(s)	1351
Bme18I	GGWCC	3 cut(s)	466, 844, 1632
BmeRI	GACNNNNNGTC	2 cut(s)	842, 1371
BmgT120I	GGNCC	5 cut(s)	206, 216, 466, 844, 1632
BmiI	GGNNCC	4 cut(s)	217, 620, 976, 1613
BmrFI	CCNGG	1 cut(s)	1351
BmsI	GCATC	1 cut(s)	1468
BpiI	GAAGAC	1 cut(s)	12
BplI	GAGNNNNNCTC	4 cut(s)	269, 301, 473, 505
Bpu10I	CCTNAGC	1 cut(s)	443
BpuEI	CTTGAG	1 cut(s)	518
BpuMI	CCSGG	1 cut(s)	1351
BsaBI	GATNNNNATC	2 cut(s)	1199, 1283
BsaI	GGTCTC	1 cut(s)	272
BsaJI	CCNNGG	3 cut(s)	400, 723, 735
BsaWI	WCCGGW	2 cut(s)	1124, 1583
Bsc4I	CCNNNNNNNGG	8 cut(s)	214, 366, 390, 491, 565, 615, 1434, 1542
Bse3DI	GCAATG	2 cut(s)	330, 1344
Bse8I	GATNNNNATC	2 cut(s)	1199, 1283
BseDI	CCNNGG	3 cut(s)	400, 723, 735
BseGI	GGATG	5 cut(s)	26, 150, 240, 306, 776
BseJI	GATNNNNATC	2 cut(s)	1199, 1283
BseLI	CCNNNNNNNGG	8 cut(s)	214, 366, 390, 491, 565, 615, 1434, 1542
BseMI	GCAATG	2 cut(s)	330, 1344
BseMII	CTCAG	1 cut(s)	457
BseRI	GAGGAG	2 cut(s)	337, 674
BseSI	GKGCMC	1 cut(s)	977
BseXI	GCAGC	3 cut(s)	934, 1490, 1584
BseYI	CCCAGC	2 cut(s)	311, 460
BsgI	GTGCAG	1 cut(s)	529
Bsh1236I	CGCG	4 cut(s)	159, 296, 391, 429
BshFI	GGCC	8 cut(s)	207, 218, 399, 405, 441, 495, 950, 1301
BshNI	GGYRCC	1 cut(s)	974
BsiHKAI	GWGCWC	2 cut(s)	366, 835
BsiSI	CCGG	8 cut(s)	219, 367, 411, 492, 1125, 1350, 1398, 1584
BslFI	GGGAC	3 cut(s)	689, 746, 1366
BslI	CCNNNNNNNGG	8 cut(s)	214, 366, 390, 491, 565, 615, 1434, 1542
BsmAI	GTCTC	3 cut(s)	272, 349, 1379
BsmBI	CGTCTC	1 cut(s)	349
BsmFI	GGGAC	3 cut(s)	689, 746, 1366
BsnI	GGCC	8 cut(s)	207, 218, 399, 405, 441, 495, 950, 1301
Bso31I	GGTCTC	1 cut(s)	272
Bsp1286I	GDGCHC	5 cut(s)	366, 486, 835, 977, 1614
Bsp1407I	TGTACA	1 cut(s)	1461
Bsp143I	GATC	8 cut(s)	597, 618, 1155, 1200, 1284, 1488, 1540, 1712
Bsp19I	CCATGG	1 cut(s)	735
BspANI	GGCC	8 cut(s)	207, 218, 399, 405, 441, 495, 950, 1301
BspCNI	CTCAG	1 cut(s)	456
BspFNI	CGCG	4 cut(s)	159, 296, 391, 429
BspLI	GGNNCC	4 cut(s)	217, 620, 976, 1613
BspMAI	CTGCAG	1 cut(s)	1602
BspPI	GGATC	4 cut(s)	592, 613, 626, 1292
BspT107I	GGYRCC	1 cut(s)	974
BspTI	CTTAAG	1 cut(s)	1321
BspTNI	GGTCTC	1 cut(s)	272
BsrDI	GCAATG	2 cut(s)	330, 1344
BsrGI	TGTACA	1 cut(s)	1461
BssECI	CCNNGG	3 cut(s)	400, 723, 735
BssMI	GATC	8 cut(s)	597, 618, 1155, 1200, 1284, 1488, 1540, 1712
BssSI	CACGAG	1 cut(s)	869
BssT1I	CCWWGG	2 cut(s)	723, 735
Bst2BI	CACGAG	1 cut(s)	869
Bst4CI	ACNGT	4 cut(s)	565, 703, 760, 1673
Bst6I	CTCTTC	5 cut(s)	119, 357, 669, 1013, 1598
BstAFI	CTTAAG	1 cut(s)	1321
BstAUI	TGTACA	1 cut(s)	1461
BstC8I	GCNNGC	3 cut(s)	298, 1191, 1254
BstDEI	CTNAG	2 cut(s)	443, 1112
BstDSI	CCRYGG	2 cut(s)	400, 735
BstEII	GGTNACC	1 cut(s)	1579
BstF5I	GGATG	5 cut(s)	26, 150, 240, 306, 776
BstFNI	CGCG	4 cut(s)	159, 296, 391, 429
BstHHI	GCGC	2 cut(s)	1244, 1483
BstKTI	GATC	8 cut(s)	600, 621, 1158, 1203, 1287, 1491, 1543, 1715
BstMAI	GTCTC	3 cut(s)	272, 349, 1379
BstMBI	GATC	8 cut(s)	597, 618, 1155, 1200, 1284, 1488, 1540, 1712
BstMWI	GCNNNNNNNGC	4 cut(s)	411, 501, 1186, 1478
BstNSI	RCATGY	1 cut(s)	1575
BstPI	GGTNACC	1 cut(s)	1579
BstSCI	CCNGG	1 cut(s)	1349
BstSFI	CTRYAG	1 cut(s)	1598
BstSLI	GKGCMC	1 cut(s)	977
BstUI	CGCG	4 cut(s)	159, 296, 391, 429
BstV1I	GCAGC	3 cut(s)	934, 1490, 1584
BstV2I	GAAGAC	1 cut(s)	12
BstX2I	RGATCY	2 cut(s)	597, 618
BstYI	RGATCY	2 cut(s)	597, 618
BsuRI	GGCC	8 cut(s)	207, 218, 399, 405, 441, 495, 950, 1301
BtgI	CCRYGG	2 cut(s)	400, 735
BtgZI	GCGATG	2 cut(s)	174, 1605
BtsCI	GGATG	5 cut(s)	26, 150, 240, 306, 776
BtsIMutI	CAGTG	1 cut(s)	699
Cac8I	GCNNGC	3 cut(s)	298, 1191, 1254
CfoI	GCGC	2 cut(s)	1244, 1483
Cfr13I	GGNCC	5 cut(s)	206, 216, 466, 844, 1632
CseI	GACGC	1 cut(s)	889
Csp6I	GTAC	5 cut(s)	1462, 1498, 1531, 1557, 1569
CspCI	CAANNNNNGTGG	2 cut(s)	76, 111
CviAII	CATG	7 cut(s)	123, 455, 523, 736, 1572, 1576, 1616
CviQI	GTAC	5 cut(s)	1462, 1498, 1531, 1557, 1569
DdeI	CTNAG	2 cut(s)	443, 1112
DpnI	GATC	8 cut(s)	599, 620, 1157, 1202, 1286, 1490, 1542, 1714
DpnII	GATC	8 cut(s)	597, 618, 1155, 1200, 1284, 1488, 1540, 1712
DrdI	GACNNNNNNGTC	2 cut(s)	464, 535
DriI	GACNNNNNGTC	2 cut(s)	842, 1371
DseDI	GACNNNNNNGTC	2 cut(s)	464, 535
EaeI	YGGCCR	2 cut(s)	397, 1299
Eam1104I	CTCTTC	5 cut(s)	119, 357, 669, 1013, 1598
Eam1105I	GACNNNNNGTC	2 cut(s)	842, 1371
EarI	CTCTTC	5 cut(s)	119, 357, 669, 1013, 1598
Ecl136II	GAGCTC	2 cut(s)	364, 833
Eco130I	CCWWGG	2 cut(s)	723, 735
Eco147I	AGGCCT	1 cut(s)	950
Eco24I	GRGCYC	4 cut(s)	366, 486, 835, 1614
Eco31I	GGTCTC	1 cut(s)	272
Eco47I	GGWCC	3 cut(s)	466, 844, 1632
Eco53kI	GAGCTC	2 cut(s)	364, 833
Eco91I	GGTNACC	1 cut(s)	1579
EcoICRI	GAGCTC	2 cut(s)	364, 833
EcoO65I	GGTNACC	1 cut(s)	1579
EcoT14I	CCWWGG	2 cut(s)	723, 735
EcoT22I	ATGCAT	2 cut(s)	1449, 1577
EcoT38I	GRGCYC	4 cut(s)	366, 486, 835, 1614
ErhI	CCWWGG	2 cut(s)	723, 735
Esp3I	CGTCTC	1 cut(s)	349
FaeI	CATG	7 cut(s)	126, 458, 526, 739, 1575, 1579, 1619
FaqI	GGGAC	3 cut(s)	689, 746, 1366
FatI	CATG	7 cut(s)	122, 454, 522, 735, 1571, 1575, 1615
FauI	CCCGC	1 cut(s)	206
FbaI	TGATCA	1 cut(s)	1488
FblI	GTMKAC	1 cut(s)	1221
Fnu4HI	GCNGC	7 cut(s)	249, 294, 392, 427, 923, 1504, 1598
FokI	GGATG	5 cut(s)	13, 137, 227, 293, 763
FriOI	GRGCYC	4 cut(s)	366, 486, 835, 1614
Fsp4HI	GCNGC	7 cut(s)	249, 294, 392, 427, 923, 1504, 1598
FspBI	CTAG	9 cut(s)	210, 236, 245, 267, 588, 861, 918, 1169, 1245
GlaI	GCGC	2 cut(s)	1243, 1482
GluI	GCNGC	7 cut(s)	249, 294, 392, 427, 923, 1504, 1598
GsaI	CCCAGC	2 cut(s)	315, 464
HaeIII	GGCC	8 cut(s)	207, 218, 399, 405, 441, 495, 950, 1301
HapII	CCGG	8 cut(s)	219, 367, 411, 492, 1125, 1350, 1398, 1584
HgaI	GACGC	1 cut(s)	889
HhaI	GCGC	2 cut(s)	1244, 1483
Hin1II	CATG	7 cut(s)	126, 458, 526, 739, 1575, 1579, 1619
Hin6I	GCGC	2 cut(s)	1242, 1481
HinP1I	GCGC	2 cut(s)	1242, 1481
HindIII	AAGCTT	1 cut(s)	1254
HinfI	GANTC	4 cut(s)	373, 864, 1011, 1656
HpaII	CCGG	8 cut(s)	219, 367, 411, 492, 1125, 1350, 1398, 1584
HphI	GGTGA	8 cut(s)	157, 428, 548, 650, 836, 1288, 1375, 1591
Hpy166II	GTNNAC	5 cut(s)	228, 419, 606, 1222, 1632
Hpy188I	TCNGA	6 cut(s)	361, 1155, 1338, 1537, 1545, 1700
Hpy188III	TCNNGA	9 cut(s)	277, 476, 595, 671, 861, 1015, 1475, 1648, 1710
Hpy8I	GTNNAC	5 cut(s)	228, 419, 606, 1222, 1632
Hpy99I	CGWCG	1 cut(s)	426
HpyAV	CCTTC	7 cut(s)	292, 298, 311, 458, 1064, 1613, 1695
HpyCH4III	ACNGT	4 cut(s)	565, 703, 760, 1673
HpyCH4IV	ACGT	3 cut(s)	421, 519, 941
HpyCH4V	TGCA	6 cut(s)	126, 526, 546, 1447, 1575, 1600
HpyF10VI	GCNNNNNNNGC	4 cut(s)	411, 501, 1186, 1478
HpyF3I	CTNAG	2 cut(s)	443, 1112
HpySE526I	ACGT	3 cut(s)	421, 519, 941
Hsp92II	CATG	7 cut(s)	126, 458, 526, 739, 1575, 1579, 1619
HspAI	GCGC	2 cut(s)	1242, 1481
Ksp22I	TGATCA	1 cut(s)	1488
Kzo9I	GATC	8 cut(s)	597, 618, 1155, 1200, 1284, 1488, 1540, 1712
LmnI	GCTCC	5 cut(s)	103, 369, 830, 1339, 1617
Lsp1109I	GCAGC	3 cut(s)	934, 1490, 1584
LweI	GCATC	1 cut(s)	1468
MaeI	CTAG	9 cut(s)	210, 236, 245, 267, 588, 861, 918, 1169, 1245
MaeII	ACGT	3 cut(s)	421, 519, 941
MaeIII	GTNAC	5 cut(s)	163, 554, 848, 1363, 1579
MalI	GATC	8 cut(s)	599, 620, 1157, 1202, 1286, 1490, 1542, 1714
MboI	GATC	8 cut(s)	597, 618, 1155, 1200, 1284, 1488, 1540, 1712
MflI	RGATCY	2 cut(s)	597, 618
MhlI	GDGCHC	5 cut(s)	366, 486, 835, 977, 1614
MlsI	TGGCCA	1 cut(s)	1301
MluCI	AATT	3 cut(s)	741, 798, 811
MluNI	TGGCCA	1 cut(s)	1301
MlyI	GAGTC	1 cut(s)	367
MmeI	TCCRAC	2 cut(s)	351, 924
Mox20I	TGGCCA	1 cut(s)	1301
Mph1103I	ATGCAT	2 cut(s)	1449, 1577
MroXI	GAANNNNTTC	5 cut(s)	661, 678, 1022, 1077, 1705
MscI	TGGCCA	1 cut(s)	1301
MseI	TTAA	5 cut(s)	56, 105, 797, 932, 1322
Msp20I	TGGCCA	1 cut(s)	1301
MspA1I	CMGCKG	1 cut(s)	464
MspCI	CTTAAG	1 cut(s)	1321
MspI	CCGG	8 cut(s)	219, 367, 411, 492, 1125, 1350, 1398, 1584
MspR9I	CCNGG	1 cut(s)	1351
MvnI	CGCG	4 cut(s)	159, 296, 391, 429
MwoI	GCNNNNNNNGC	4 cut(s)	411, 501, 1186, 1478
NciI	CCSGG	1 cut(s)	1351
NcoI	CCATGG	1 cut(s)	735
NdeII	GATC	8 cut(s)	597, 618, 1155, 1200, 1284, 1488, 1540, 1712
NlaIII	CATG	7 cut(s)	126, 458, 526, 739, 1575, 1579, 1619
NlaIV	GGNNCC	4 cut(s)	217, 620, 976, 1613
NmeAIII	GCCGAG	1 cut(s)	375
NmuCI	GTSAC	4 cut(s)	163, 554, 1363, 1579
NsiI	ATGCAT	2 cut(s)	1449, 1577
NspI	RCATGY	1 cut(s)	1575
PceI	AGGCCT	1 cut(s)	950
PcsI	WCGNNNNNNNCGW	1 cut(s)	1364
PdmI	GAANNNNTTC	5 cut(s)	661, 678, 1022, 1077, 1705
PfeI	GAWTC	3 cut(s)	864, 1011, 1656
PflMI	CCANNNNNTGG	1 cut(s)	1434
PkrI	GCNGC	7 cut(s)	250, 295, 393, 428, 924, 1505, 1599
PleI	GAGTC	1 cut(s)	367
PpsI	GAGTC	1 cut(s)	367
PshBI	ATTAAT	1 cut(s)	797
Psp124BI	GAGCTC	2 cut(s)	366, 835
PspEI	GGTNACC	1 cut(s)	1579
PspFI	CCCAGC	2 cut(s)	311, 460
PspN4I	GGNNCC	4 cut(s)	217, 620, 976, 1613
PspPI	GGNCC	5 cut(s)	206, 216, 466, 844, 1632
PstI	CTGCAG	1 cut(s)	1602
PsuI	RGATCY	2 cut(s)	597, 618
RsaI	GTAC	5 cut(s)	1463, 1499, 1532, 1558, 1570
RsaNI	GTAC	5 cut(s)	1462, 1498, 1531, 1557, 1569
SacI	GAGCTC	2 cut(s)	366, 835
SaqAI	TTAA	5 cut(s)	56, 105, 797, 932, 1322
SatI	GCNGC	7 cut(s)	249, 294, 392, 427, 923, 1504, 1598
Sau3AI	GATC	8 cut(s)	597, 618, 1155, 1200, 1284, 1488, 1540, 1712
Sau96I	GGNCC	5 cut(s)	206, 216, 466, 844, 1632
SchI	GAGTC	1 cut(s)	367
ScrFI	CCNGG	1 cut(s)	1351
SduI	GDGCHC	5 cut(s)	366, 486, 835, 977, 1614
SfaNI	GCATC	1 cut(s)	1468
SfcI	CTRYAG	1 cut(s)	1598
SinI	GGWCC	3 cut(s)	466, 844, 1632
SmlI	CTYRAG	2 cut(s)	533, 1321
SmoI	CTYRAG	2 cut(s)	533, 1321
Sse9I	AATT	3 cut(s)	741, 798, 811
SseBI	AGGCCT	1 cut(s)	950
SspMI	CTAG	9 cut(s)	210, 236, 245, 267, 588, 861, 918, 1169, 1245
SstI	GAGCTC	2 cut(s)	366, 835
StuI	AGGCCT	1 cut(s)	950
StyD4I	CCNGG	1 cut(s)	1349
StyI	CCWWGG	2 cut(s)	723, 735
TaaI	ACNGT	4 cut(s)	565, 703, 760, 1673
TaiI	ACGT	3 cut(s)	424, 522, 944
TaqI	TCGA	5 cut(s)	276, 498, 632, 835, 1014
TaqII	GACCGA	1 cut(s)	1016
TasI	AATT	3 cut(s)	741, 798, 811
TatI	WGTACW	3 cut(s)	1461, 1556, 1568
TauI	GCSGC	4 cut(s)	251, 296, 394, 429
TfiI	GAWTC	3 cut(s)	864, 1011, 1656
Tru1I	TTAA	5 cut(s)	56, 105, 797, 932, 1322
Tru9I	TTAA	5 cut(s)	56, 105, 797, 932, 1322
TscAI	CASTG	1 cut(s)	706
TseFI	GTSAC	4 cut(s)	163, 554, 1363, 1579
TseI	GCWGC	3 cut(s)	922, 1503, 1597
Tsp45I	GTSAC	4 cut(s)	163, 554, 1363, 1579
TspDTI	ATGAA	3 cut(s)	54, 1078, 1632
TspGWI	ACGGA	2 cut(s)	669, 836
TspRI	CASTG	1 cut(s)	706
Van91I	CCANNNNNTGG	1 cut(s)	1434
Vha464I	CTTAAG	1 cut(s)	1321
VpaK11BI	GGWCC	3 cut(s)	466, 844, 1632
VspI	ATTAAT	1 cut(s)	797
XapI	RAATTY	2 cut(s)	741, 811
XbaI	TCTAGA	1 cut(s)	860
XceI	RCATGY	1 cut(s)	1575
XmiI	GTMKAC	1 cut(s)	1221
XmnI	GAANNNNTTC	5 cut(s)	661, 678, 1022, 1077, 1705
XspI	CTAG	9 cut(s)	210, 236, 245, 267, 588, 861, 918, 1169, 1245
Zsp2I	ATGCAT	2 cut(s)	1449, 1577

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.