Rroxscaffold_4G00293690

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018477
Physical Location & Seq
Forward (+)
13899050 .. 13901174
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00293690.1

Sequence Viewer

Length: 855 bp
ATGGATCCCTACACGCAACTCACCAGTACACACGGCCTCAGAGTCTTCCAACTCTTGGAGCTCGAGAAGGCCACCAAGAACTTCAGCTCGTCTCGCTACGTTGGAGGGGGAGATTTTGGAGCTGAGATCTTTAAGGGAGTGATCAAGAATGCCAACAAAACAAAACTTCATGTACTGGTCTATAGGATCACCCGAGGACCTACTCTTGAGGCAAAAAGCGACGAAGAGTGGGCGAGGGAGCTCACAAAACAAGTATCAGCTATTGATGATCATCCAAACTTTGTTAGAGTGGTGGGATATTGTGCCACTCGATACAAGAAGAAGAAGAAGGGACATGCAGTGCCTGTACGATTTATAGTGGAAGAAGATGCATGCAATGGAAATTTACATGACTATCTACATGAGGGGCGGCATCAACCTCTTTCATGGGCAAAGAGGCTCAGCATACTCCGGGATGTTGCTCGTGGTTTGGCTTATTTTCATGATTCATTAGATAACAACGACGTTACATGTGCTTCTGTGAGTTATGAAGCTCATGAACTCCTTCTAAGAGGCGACCTGACGTCCAAGAGCAACGTGTGGAGCTATGGGGTCTTTATCTTGGAGGTCATAACTGGTAACCAGCCATCTGATGGAAACCTAACGAAAAAGTTAAAGAAATTATGGAGCTCCGATAGAAACAAGTTGGAGAAGATGATAGACCCCAAGCTTAAAGGAAGGTATTCTGTAGAATCGGCAATGGCACTAGCAGCACTTGCAGCTGATCATTGCTTGCAGGAAGAGCCAAACAAGCGACCTAAAATGAGTGAGGTCTTTGCAATGGTGTCTACAATGGCAGAGACAGCAGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

284

Amino Acids

31.99

Weight (kDa)

8.86

Isoelectric Point (pI)

29.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 31 - 162 2.4e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 168 - 272 1.8e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 172 - 272 1.2e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018477)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18940
rosa_chinensis RchiOBHm_Chr1g0362871
rosa_multiflora Rmu_sc0001502.1_g000013
rosa_roxburghii Rroxscaffold_4G00293690
rosa_rugosa Rorug01G0302100
rosa_samantha Rh1AG311000 Rh1BG274100 Rh1CG291300 Rh1DG304900
rosa_wichuraiana Rw1G027570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 566
AccB7I CCANNNNNTGG 2 cut(s) 55, 632
AccI GTMKAC 1 cut(s) 827
AciI CCGC 1 cut(s) 409
AclWI GGATC 2 cut(s) 12, 194
AcsI RAATTY 2 cut(s) 382, 849
AcuI CTGAAG 1 cut(s) 67
AcyI GRCGYC 1 cut(s) 563
AfaI GTAC 3 cut(s) 28, 174, 348
AfiI CCNNNNNNNGG 2 cut(s) 55, 632
AflIII ACRYGT 2 cut(s) 509, 576
AhdI GACNNNNNGTC 1 cut(s) 562
Alw21I GWGCWC 3 cut(s) 63, 243, 671
Alw26I GTCTC 2 cut(s) 96, 833
AlwI GGATC 2 cut(s) 12, 194
AlwNI CAGNNNCTG 1 cut(s) 344
Ama87I CYCGRG 2 cut(s) 62, 192
AoxI GGCC 2 cut(s) 34, 69
ApeKI GCWGC 2 cut(s) 749, 758
ApoI RAATTY 2 cut(s) 382, 849
Asp700I GAANNNNTTC 2 cut(s) 543, 721
AspS9I GGNCC 1 cut(s) 197
AsuC2I CCSGG 1 cut(s) 452
AsuHPI GGTGA 2 cut(s) 13, 181
AvaI CYCGRG 2 cut(s) 62, 192
AvaII GGWCC 1 cut(s) 197
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 63, 243, 671
BauI CACGAG 1 cut(s) 462
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 3 cut(s) 63, 243, 671
BbvI GCAGC 2 cut(s) 761, 770
BccI CCATC 2 cut(s) 626, 634
BceAI ACGGC 1 cut(s) 49
BclI TGATCA 3 cut(s) 141, 268, 763
BcnI CCSGG 1 cut(s) 452
BcoDI GTCTC 2 cut(s) 96, 833
BfaI CTAG 1 cut(s) 746
BfmI CTRYAG 2 cut(s) 181, 726
BglII AGATCT 1 cut(s) 126
BisI GCNGC 3 cut(s) 410, 750, 759
BlpI GCTNAGC 1 cut(s) 440
BlsI GCNGC 3 cut(s) 411, 751, 760
Bme1390I CCNGG 1 cut(s) 452
Bme18I GGWCC 1 cut(s) 197
BmeRI GACNNNNNGTC 1 cut(s) 562
BmeT110I CYCGRG 2 cut(s) 62, 192
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 452
BmsI GCATC 2 cut(s) 358, 421
BpiI GAAGAC 1 cut(s) 37
Bpu1102I GCTNAGC 1 cut(s) 440
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 452
BsaBI GATNNNNATC 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 563
BsaJI CCNNGG 1 cut(s) 193
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 632
Bse1I ACTGG 3 cut(s) 24, 180, 619
Bse3DI GCAATG 4 cut(s) 382, 744, 766, 825
Bse8I GATNNNNATC 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 2 cut(s) 271, 460
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 2 cut(s) 55, 632
BseMI GCAATG 4 cut(s) 382, 744, 766, 825
BseMII CTCAG 3 cut(s) 52, 114, 454
BseNI ACTGG 3 cut(s) 24, 180, 619
BseXI GCAGC 2 cut(s) 761, 770
BshFI GGCC 2 cut(s) 36, 71
BsiHKAI GWGCWC 3 cut(s) 63, 243, 671
BsiHKCI CYCGRG 2 cut(s) 62, 192
BsiSI CCGG 1 cut(s) 451
BslFI GGGAC 1 cut(s) 345
BslI CCNNNNNNNGG 2 cut(s) 55, 632
BsmAI GTCTC 2 cut(s) 96, 833
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 345
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 2 cut(s) 36, 71
BsoBI CYCGRG 2 cut(s) 62, 192
Bsp1286I GDGCHC 3 cut(s) 63, 243, 671
Bsp143I GATC 6 cut(s) 4, 126, 141, 186, 268, 763
Bsp1720I GCTNAGC 1 cut(s) 440
BspACI CCGC 1 cut(s) 409
BspANI GGCC 2 cut(s) 36, 71
BspCNI CTCAG 3 cut(s) 51, 115, 453
BspHI TCATGA 2 cut(s) 481, 535
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 12, 194
BspQI GCTCTTC 1 cut(s) 774
BsrDI GCAATG 4 cut(s) 382, 744, 766, 825
BsrI ACTGG 3 cut(s) 24, 180, 619
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 6 cut(s) 4, 126, 141, 186, 268, 763
BssNI GRCGYC 1 cut(s) 563
BssSI CACGAG 1 cut(s) 462
Bst2BI CACGAG 1 cut(s) 462
Bst6I CTCTTC 2 cut(s) 219, 774
BstACI GRCGYC 1 cut(s) 563
BstAPI GCANNNNNTGC 1 cut(s) 755
BstC8I GCNNGC 2 cut(s) 373, 773
BstDEI CTNAG 4 cut(s) 38, 123, 440, 548
BstEII GGTNACC 1 cut(s) 617
BstF5I GGATG 2 cut(s) 271, 460
BstKTI GATC 6 cut(s) 7, 129, 144, 189, 271, 766
BstMAI GTCTC 2 cut(s) 96, 833
BstMBI GATC 6 cut(s) 4, 126, 141, 186, 268, 763
BstMWI GCNNNNNNNGC 7 cut(s) 93, 749, 755, 758, 781, 790, 842
BstNSI RCATGY 3 cut(s) 338, 375, 513
BstPI GGTNACC 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 450
BstSFI CTRYAG 2 cut(s) 181, 726
BstV1I GCAGC 2 cut(s) 761, 770
BstV2I GAAGAC 1 cut(s) 37
BstX2I RGATCY 2 cut(s) 4, 126
BstYI RGATCY 2 cut(s) 4, 126
BsuRI GGCC 2 cut(s) 36, 71
BtsCI GGATG 2 cut(s) 271, 460
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 1 cut(s) 345
Cac8I GCNNGC 2 cut(s) 373, 773
CaiI CAGNNNCTG 1 cut(s) 344
CciI TCATGA 2 cut(s) 481, 535
Cfr13I GGNCC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 27, 173, 347
CviAII CATG 9 cut(s) 170, 335, 372, 389, 401, 426, 482, 510, 536
CviQI GTAC 3 cut(s) 27, 173, 347
DdeI CTNAG 4 cut(s) 38, 123, 440, 548
DpnI GATC 6 cut(s) 6, 128, 143, 188, 270, 765
DpnII GATC 6 cut(s) 4, 126, 141, 186, 268, 763
DriI GACNNNNNGTC 1 cut(s) 562
Eam1104I CTCTTC 2 cut(s) 219, 774
Eam1105I GACNNNNNGTC 1 cut(s) 562
EarI CTCTTC 2 cut(s) 219, 774
Ecl136II GAGCTC 3 cut(s) 61, 241, 669
Eco24I GRGCYC 3 cut(s) 63, 243, 671
Eco47I GGWCC 1 cut(s) 197
Eco53kI GAGCTC 3 cut(s) 61, 241, 669
Eco57I CTGAAG 1 cut(s) 67
Eco88I CYCGRG 2 cut(s) 62, 192
Eco91I GGTNACC 1 cut(s) 617
EcoICRI GAGCTC 3 cut(s) 61, 241, 669
EcoO109I RGGNCCY 1 cut(s) 197
EcoO65I GGTNACC 1 cut(s) 617
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 3 cut(s) 63, 243, 671
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 9 cut(s) 173, 338, 375, 392, 404, 429, 485, 513, 539
FaqI GGGAC 1 cut(s) 345
FatI CATG 9 cut(s) 169, 334, 371, 388, 400, 425, 481, 509, 535
FbaI TGATCA 3 cut(s) 141, 268, 763
FblI GTMKAC 1 cut(s) 827
Fnu4HI GCNGC 3 cut(s) 410, 750, 759
FokI GGATG 2 cut(s) 258, 467
FriOI GRGCYC 3 cut(s) 63, 243, 671
Fsp4HI GCNGC 3 cut(s) 410, 750, 759
FspBI CTAG 1 cut(s) 746
GluI GCNGC 3 cut(s) 410, 750, 759
HaeIII GGCC 2 cut(s) 36, 71
HapII CCGG 1 cut(s) 451
Hin1I GRCGYC 1 cut(s) 563
Hin1II CATG 9 cut(s) 173, 338, 375, 392, 404, 429, 485, 513, 539
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 3 cut(s) 42, 485, 731
HpaII CCGG 1 cut(s) 451
HphI GGTGA 2 cut(s) 13, 181
Hpy166II GTNNAC 2 cut(s) 29, 828
Hpy188I TCNGA 3 cut(s) 41, 631, 673
Hpy188III TCNNGA 5 cut(s) 64, 145, 206, 482, 536
Hpy8I GTNNAC 2 cut(s) 29, 828
Hpy99I CGWCG 2 cut(s) 224, 506
HpyAV CCTTC 4 cut(s) 61, 322, 554, 711
HpyCH4IV ACGT 4 cut(s) 99, 504, 563, 576
HpyCH4V TGCA 6 cut(s) 338, 371, 375, 758, 775, 818
HpyF10VI GCNNNNNNNGC 7 cut(s) 93, 749, 755, 758, 781, 790, 842
HpyF3I CTNAG 4 cut(s) 38, 123, 440, 548
HpySE526I ACGT 4 cut(s) 99, 504, 563, 576
Hsp92I GRCGYC 1 cut(s) 563
Hsp92II CATG 9 cut(s) 173, 338, 375, 392, 404, 429, 485, 513, 539
Ksp22I TGATCA 3 cut(s) 141, 268, 763
Kzo9I GATC 6 cut(s) 4, 126, 141, 186, 268, 763
LguI GCTCTTC 1 cut(s) 774
LmnI GCTCC 6 cut(s) 58, 119, 238, 582, 666, 674
LpnPI CCDG 8 cut(s) 37, 161, 357, 464, 572, 600, 635, 761
Lsp1109I GCAGC 2 cut(s) 761, 770
LweI GCATC 2 cut(s) 358, 421
MaeI CTAG 1 cut(s) 746
MaeII ACGT 4 cut(s) 99, 504, 563, 576
MaeIII GTNAC 2 cut(s) 505, 617
MalI GATC 6 cut(s) 6, 128, 143, 188, 270, 765
MboI GATC 6 cut(s) 4, 126, 141, 186, 268, 763
MboII GAAGA 9 cut(s) 37, 236, 331, 334, 337, 374, 377, 703, 791
MflI RGATCY 2 cut(s) 4, 126
MhlI GDGCHC 3 cut(s) 63, 243, 671
MluCI AATT 3 cut(s) 382, 659, 849
MlyI GAGTC 1 cut(s) 51
MmeI TCCRAC 3 cut(s) 73, 82, 666
Mph1103I ATGCAT 1 cut(s) 373
MroXI GAANNNNTTC 2 cut(s) 543, 721
MseI TTAA 3 cut(s) 132, 653, 711
MspA1I CMGCKG 1 cut(s) 761
MspI CCGG 1 cut(s) 451
MspR9I CCNGG 1 cut(s) 452
Mva1269I GAATGC 1 cut(s) 154
MwoI GCNNNNNNNGC 7 cut(s) 93, 749, 755, 758, 781, 790, 842
NciI CCSGG 1 cut(s) 452
NdeII GATC 6 cut(s) 4, 126, 141, 186, 268, 763
NlaIII CATG 9 cut(s) 173, 338, 375, 392, 404, 429, 485, 513, 539
NlaIV GGNNCC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 373
NspI RCATGY 3 cut(s) 338, 375, 513
PaeI GCATGC 1 cut(s) 375
PaeR7I CTCGAG 1 cut(s) 62
PagI TCATGA 2 cut(s) 481, 535
PciI ACATGT 1 cut(s) 509
PciSI GCTCTTC 1 cut(s) 774
PctI GAATGC 1 cut(s) 154
PdmI GAANNNNTTC 2 cut(s) 543, 721
PfeI GAWTC 2 cut(s) 485, 731
PflMI CCANNNNNTGG 2 cut(s) 55, 632
PfoI TCCNGGA 1 cut(s) 450
PkrI GCNGC 3 cut(s) 411, 751, 760
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 197
PscI ACATGT 1 cut(s) 509
Psp124BI GAGCTC 3 cut(s) 63, 243, 671
Psp5II RGGWCCY 1 cut(s) 197
PspEI GGTNACC 1 cut(s) 617
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 197
PspPPI RGGWCCY 1 cut(s) 197
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 2 cut(s) 4, 126
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 3 cut(s) 28, 174, 348
RsaNI GTAC 3 cut(s) 27, 173, 347
SacI GAGCTC 3 cut(s) 63, 243, 671
SapI GCTCTTC 1 cut(s) 774
SaqAI TTAA 3 cut(s) 132, 653, 711
SatI GCNGC 3 cut(s) 410, 750, 759
Sau3AI GATC 6 cut(s) 4, 126, 141, 186, 268, 763
Sau96I GGNCC 1 cut(s) 197
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 1 cut(s) 452
SduI GDGCHC 3 cut(s) 63, 243, 671
SfaNI GCATC 2 cut(s) 358, 421
SfcI CTRYAG 2 cut(s) 181, 726
Sfr274I CTCGAG 1 cut(s) 62
SinI GGWCC 1 cut(s) 197
SlaI CTCGAG 1 cut(s) 62
SmlI CTYRAG 2 cut(s) 62, 206
SmoI CTYRAG 2 cut(s) 62, 206
SphI GCATGC 1 cut(s) 375
Sse9I AATT 3 cut(s) 382, 659, 849
SsiI CCGC 1 cut(s) 409
SspMI CTAG 1 cut(s) 746
SstI GAGCTC 3 cut(s) 63, 243, 671
StyD4I CCNGG 1 cut(s) 450
TaiI ACGT 4 cut(s) 102, 507, 566, 579
TaqI TCGA 2 cut(s) 63, 310
TasI AATT 3 cut(s) 382, 659, 849
TatI WGTACW 2 cut(s) 26, 172
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 2 cut(s) 485, 731
Tru1I TTAA 3 cut(s) 132, 653, 711
Tru9I TTAA 3 cut(s) 132, 653, 711
TscAI CASTG 1 cut(s) 345
TseI GCWGC 2 cut(s) 749, 758
TspDTI ATGAA 6 cut(s) 158, 414, 470, 477, 543, 552
TspRI CASTG 1 cut(s) 345
Van91I CCANNNNNTGG 2 cut(s) 55, 632
VpaK11BI GGWCC 1 cut(s) 197
XapI RAATTY 2 cut(s) 382, 849
XceI RCATGY 3 cut(s) 338, 375, 513
XcmI CCANNNNNNNNNTGG 1 cut(s) 629
XhoI CTCGAG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 827
XmnI GAANNNNTTC 2 cut(s) 543, 721
XspI CTAG 1 cut(s) 746
ZraI GACGTC 1 cut(s) 564
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.