Rorug01G0302100

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001624
Physical Location & Seq
Reverse (-)
41805740 .. 41810279
4540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0302100.1

Sequence Viewer

Length: 954 bp
ATGGGGCATTCATCAGCAGTCTGGGACTGTAAGGCAGCAATTGAGAAAACAAAGGACTTGAATGGGGTCGGACAAGTTGTTCTTCGAAACCCACAAGGGGCTTCAGCGCAGGTTAGCTTGCATGGAGGACAGGTCACATCATGGAGGAATGAGCATGGAGAAGAACTCCTATTCACTAGTAGTAAGGCAATCTTCAAGCCTCCAAAAGCAATGCGAGGAGGAATTCCCATTTGTTTCCCACAGTTTGGGAATTGTGGATCTTTAGAACTACATGGTTTTGCAAGAAACAAGTTCTGGGCAATTGATGATAATCCTCCAGTGCTTCGCCCAAATGATTCCCAAGGAAAATCATTTGTTGATCTACTTCTTAAACCATCTGAAGAGGACCTGAAGTTCTGGCCCCATAGTTTTGAGTTTCGCCTAAGGGTGTCTCTTGCAATAAATGGAGATCTGACCCTGATATCACGAGTCAGGAATGTGAATGGCAAGATGTTTAATTTCTCTTTTGCCTACCACACTTACTTTTTGGTTTCTGACATAAGTGAGGTGAGGATCGAAGGGCTGGAGACATGTGACTACTTAGACAACCTTTGTCACAGAGAACGTTTCACAGAACAAGGAGATGCCTTAACTTTTGAATCAGAGGTTGATCGACTTTATCTAAGTTCACGCAAGGTAATTGCTGTTCTTGATCATGAAAAAAAACAGACATATGTAATCAGAAAGGAAGGACTACCAGATGTTGTTGTTTGGAATCCGTGGGAGAAGAAATCAAAATCCATGGTTGATTTTGGTGACGAGGAATATAAGCAGATGCTTTGTGTGGATGCTGCGGCAATTGAGAAACCCATCACCCTGAAGCCAGGAGAGGAATGGACAGGGAGGTTGCAGCTCTCTGTTGTGCCTTCGAGTTTCTGTAGTGAGAACCTGGATCTTGAGAAGGGAGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

317

Amino Acids

35.82

Weight (kDa)

5.82

Isoelectric Point (pI)

31.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 25 - 298 6.1e-70 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 100
AccB7I CCANNNNNTGG 1 cut(s) 245
AciI CCGC 1 cut(s) 833
AclI AACGTT 1 cut(s) 604
AclWI GGATC 3 cut(s) 265, 560, 939
AcsI RAATTY 1 cut(s) 222
AcuI CTGAAG 4 cut(s) 87, 399, 410, 878
AfiI CCNNNNNNNGG 2 cut(s) 97, 245
AflIII ACRYGT 1 cut(s) 569
AgsI TTSAA 3 cut(s) 61, 196, 638
AhlI ACTAGT 1 cut(s) 176
AjnI CCWGG 2 cut(s) 862, 927
AloI GAACNNNNNNTCC 4 cut(s) 63, 95, 377, 409
AluBI AGCT 2 cut(s) 117, 892
AluI AGCT 2 cut(s) 117, 892
Alw26I GTCTC 2 cut(s) 435, 560
AlwI GGATC 3 cut(s) 265, 560, 939
AoxI GGCC 1 cut(s) 398
ApeKI GCWGC 3 cut(s) 35, 830, 889
ApoI RAATTY 1 cut(s) 222
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 2 cut(s) 385, 399
AsuHPI GGTGA 3 cut(s) 559, 806, 844
AsuII TTCGAA 1 cut(s) 85
AvaII GGWCC 1 cut(s) 385
AxyI CCTNAGG 1 cut(s) 422
BauI CACGAG 1 cut(s) 465
BbvI GCAGC 3 cut(s) 47, 817, 901
BccI CCATC 2 cut(s) 382, 857
BciT130I CCWGG 2 cut(s) 864, 929
BclI TGATCA 1 cut(s) 691
BcoDI GTCTC 2 cut(s) 435, 560
BcuI ACTAGT 1 cut(s) 176
BfaI CTAG 1 cut(s) 177
BfmI CTRYAG 1 cut(s) 916
BfuAI ACCTGC 1 cut(s) 100
BglII AGATCT 1 cut(s) 448
BisI GCNGC 4 cut(s) 36, 831, 834, 890
BlsI GCNGC 4 cut(s) 37, 832, 835, 891
Bme1390I CCNGG 2 cut(s) 864, 929
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 2 cut(s) 385, 399
BmiI GGNNCC 1 cut(s) 401
BmrFI CCNGG 2 cut(s) 864, 929
BmsI GCATC 3 cut(s) 613, 804, 817
BplI GAGNNNNNCTC 2 cut(s) 150, 182
BpmI CTGGAG 2 cut(s) 300, 584
Bpu14I TTCGAA 1 cut(s) 85
BsaBI GATNNNNATC 1 cut(s) 309
BsaJI CCNNGG 3 cut(s) 340, 758, 780
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 245
Bse1I ACTGG 1 cut(s) 317
Bse21I CCTNAGG 1 cut(s) 422
Bse3DI GCAATG 1 cut(s) 216
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 2 cut(s) 864, 929
BseDI CCNNGG 3 cut(s) 340, 758, 780
BseGI GGATG 1 cut(s) 832
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 2 cut(s) 97, 245
BseMI GCAATG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 3 cut(s) 47, 817, 901
BshFI GGCC 1 cut(s) 400
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 2 cut(s) 97, 245
BsmAI GTCTC 2 cut(s) 435, 560
BsmFI GGGAC 1 cut(s) 38
BsmI GAATGC 1 cut(s) 7
BsnI GGCC 1 cut(s) 400
Bsp119I TTCGAA 1 cut(s) 85
Bsp143I GATC 7 cut(s) 257, 358, 448, 552, 649, 691, 931
Bsp19I CCATGG 1 cut(s) 780
BspACI CCGC 1 cut(s) 833
BspANI GGCC 1 cut(s) 400
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 1 cut(s) 401
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 3 cut(s) 265, 560, 939
BspT104I TTCGAA 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 1 cut(s) 317
BssECI CCNNGG 3 cut(s) 340, 758, 780
BssMI GATC 7 cut(s) 257, 358, 448, 552, 649, 691, 931
BssSI CACGAG 1 cut(s) 465
BssT1I CCWWGG 2 cut(s) 340, 780
Bst2BI CACGAG 1 cut(s) 465
Bst2UI CCWGG 2 cut(s) 864, 929
Bst4CI ACNGT 2 cut(s) 29, 243
Bst6I CTCTTC 1 cut(s) 375
BstBI TTCGAA 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 3 cut(s) 422, 580, 662
BstDSI CCRYGG 2 cut(s) 758, 780
BstF5I GGATG 1 cut(s) 832
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 7 cut(s) 260, 361, 451, 555, 652, 694, 934
BstMAI GTCTC 2 cut(s) 435, 560
BstMBI GATC 7 cut(s) 257, 358, 448, 552, 649, 691, 931
BstNI CCWGG 2 cut(s) 864, 929
BstNSI RCATGY 1 cut(s) 573
BstSCI CCNGG 2 cut(s) 862, 927
BstSFI CTRYAG 1 cut(s) 916
BstV1I GCAGC 3 cut(s) 47, 817, 901
BstX2I RGATCY 3 cut(s) 257, 448, 931
BstYI RGATCY 3 cut(s) 257, 448, 931
Bsu36I CCTNAGG 1 cut(s) 422
BsuRI GGCC 1 cut(s) 400
BtgI CCRYGG 2 cut(s) 758, 780
BtsCI GGATG 1 cut(s) 832
BtsIMutI CAGTG 1 cut(s) 324
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 119
CciI TCATGA 1 cut(s) 694
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 385, 399
CviAII CATG 7 cut(s) 122, 141, 155, 272, 570, 695, 781
CviJI RGCY 7 cut(s) 101, 117, 199, 400, 562, 862, 892
CviKI_1 RGCY 7 cut(s) 101, 117, 199, 400, 562, 862, 892
DdeI CTNAG 3 cut(s) 422, 580, 662
DpnI GATC 7 cut(s) 259, 360, 450, 554, 651, 693, 933
DpnII GATC 7 cut(s) 257, 358, 448, 552, 649, 691, 931
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 2 cut(s) 340, 780
Eco32I GATATC 1 cut(s) 462
Eco47I GGWCC 1 cut(s) 385
Eco57I CTGAAG 4 cut(s) 87, 399, 410, 878
Eco81I CCTNAGG 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 385
EcoRI GAATTC 1 cut(s) 222
EcoRII CCWGG 2 cut(s) 862, 927
EcoRV GATATC 1 cut(s) 462
EcoT14I CCWWGG 2 cut(s) 340, 780
ErhI CCWWGG 2 cut(s) 340, 780
FaeI CATG 7 cut(s) 125, 144, 158, 275, 573, 698, 784
FalI AAGNNNNNCTT 4 cut(s) 66, 98, 176, 208
FaqI GGGAC 1 cut(s) 38
FatI CATG 7 cut(s) 121, 140, 154, 271, 569, 694, 780
FauNDI CATATG 1 cut(s) 712
FbaI TGATCA 1 cut(s) 691
Fnu4HI GCNGC 4 cut(s) 36, 831, 834, 890
FokI GGATG 1 cut(s) 839
Fsp4HI GCNGC 4 cut(s) 36, 831, 834, 890
FspBI CTAG 1 cut(s) 177
GlaI GCGC 1 cut(s) 108
GluI GCNGC 4 cut(s) 36, 831, 834, 890
GsuI CTGGAG 2 cut(s) 300, 584
HaeIII GGCC 1 cut(s) 400
HhaI GCGC 1 cut(s) 109
Hin1II CATG 7 cut(s) 125, 144, 158, 275, 573, 698, 784
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HinfI GANTC 4 cut(s) 335, 468, 638, 754
HphI GGTGA 3 cut(s) 559, 806, 844
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 6 cut(s) 71, 379, 453, 535, 643, 722
Hpy188III TCNNGA 5 cut(s) 465, 472, 689, 695, 935
Hpy8I GTNNAC 1 cut(s) 668
HpyAV CCTTC 4 cut(s) 551, 722, 915, 934
HpyCH4III ACNGT 2 cut(s) 29, 243
HpyCH4IV ACGT 1 cut(s) 604
HpyCH4V TGCA 4 cut(s) 121, 281, 437, 889
HpyF3I CTNAG 3 cut(s) 422, 580, 662
HpySE526I ACGT 1 cut(s) 604
Hsp92II CATG 7 cut(s) 125, 144, 158, 275, 573, 698, 784
HspAI GCGC 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 7 cut(s) 257, 358, 448, 552, 649, 691, 931
Lsp1109I GCAGC 3 cut(s) 47, 817, 901
LweI GCATC 3 cut(s) 613, 804, 817
MaeI CTAG 1 cut(s) 177
MaeII ACGT 1 cut(s) 604
MaeIII GTNAC 4 cut(s) 133, 572, 593, 794
MalI GATC 7 cut(s) 259, 360, 450, 554, 651, 693, 933
MboI GATC 7 cut(s) 257, 358, 448, 552, 649, 691, 931
MboII GAAGA 5 cut(s) 74, 173, 184, 392, 778
MfeI CAATTG 3 cut(s) 39, 300, 837
MflI RGATCY 3 cut(s) 257, 448, 931
MluCI AATT 7 cut(s) 39, 222, 250, 300, 496, 678, 837
MlyI GAGTC 1 cut(s) 477
MmeI TCCRAC 1 cut(s) 49
MseI TTAA 3 cut(s) 369, 495, 629
MspR9I CCNGG 2 cut(s) 864, 929
MunI CAATTG 3 cut(s) 39, 300, 837
Mva1269I GAATGC 1 cut(s) 7
MvaI CCWGG 2 cut(s) 864, 929
NcoI CCATGG 1 cut(s) 780
NdeI CATATG 1 cut(s) 712
NdeII GATC 7 cut(s) 257, 358, 448, 552, 649, 691, 931
NlaIII CATG 7 cut(s) 125, 144, 158, 275, 573, 698, 784
NlaIV GGNNCC 1 cut(s) 401
NmuCI GTSAC 4 cut(s) 133, 572, 593, 794
NspI RCATGY 1 cut(s) 573
NspV TTCGAA 1 cut(s) 85
PagI TCATGA 1 cut(s) 694
PciI ACATGT 1 cut(s) 569
PctI GAATGC 1 cut(s) 7
PfeI GAWTC 3 cut(s) 335, 638, 754
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 4 cut(s) 37, 832, 835, 891
PleI GAGTC 1 cut(s) 476
PpsI GAGTC 1 cut(s) 476
PpuMI RGGWCCY 1 cut(s) 385
PscI ACATGT 1 cut(s) 569
Psp1406I AACGTT 1 cut(s) 604
Psp5II RGGWCCY 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 862, 927
PspGI CCWGG 2 cut(s) 862, 927
PspN4I GGNNCC 1 cut(s) 401
PspPI GGNCC 2 cut(s) 385, 399
PspPPI RGGWCCY 1 cut(s) 385
PsrI GAACNNNNNNTAC 2 cut(s) 669, 701
PsuI RGATCY 3 cut(s) 257, 448, 931
SaqAI TTAA 3 cut(s) 369, 495, 629
SatI GCNGC 4 cut(s) 36, 831, 834, 890
Sau3AI GATC 7 cut(s) 257, 358, 448, 552, 649, 691, 931
Sau96I GGNCC 2 cut(s) 385, 399
SchI GAGTC 1 cut(s) 477
ScrFI CCNGG 2 cut(s) 864, 929
SfaNI GCATC 3 cut(s) 613, 804, 817
SfcI CTRYAG 1 cut(s) 916
SfuI TTCGAA 1 cut(s) 85
SinI GGWCC 1 cut(s) 385
SmlI CTYRAG 1 cut(s) 935
SmoI CTYRAG 1 cut(s) 935
SpeI ACTAGT 1 cut(s) 176
Sse9I AATT 7 cut(s) 39, 222, 250, 300, 496, 678, 837
SsiI CCGC 1 cut(s) 833
SspMI CTAG 1 cut(s) 177
StyD4I CCNGG 2 cut(s) 862, 927
StyI CCWWGG 2 cut(s) 340, 780
TaaI ACNGT 2 cut(s) 29, 243
TaiI ACGT 1 cut(s) 607
TaqI TCGA 4 cut(s) 85, 555, 652, 908
TasI AATT 7 cut(s) 39, 222, 250, 300, 496, 678, 837
TauI GCSGC 1 cut(s) 836
TfiI GAWTC 3 cut(s) 335, 638, 754
Tru1I TTAA 3 cut(s) 369, 495, 629
Tru9I TTAA 3 cut(s) 369, 495, 629
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 4 cut(s) 133, 572, 593, 794
TseI GCWGC 3 cut(s) 35, 830, 889
Tsp45I GTSAC 4 cut(s) 133, 572, 593, 794
TspDTI ATGAA 1 cut(s) 711
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 1 cut(s) 324
Van91I CCANNNNNTGG 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 1 cut(s) 222
XceI RCATGY 1 cut(s) 573
XcmI CCANNNNNNNNNTGG 1 cut(s) 870
XspI CTAG 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.