Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0001624

Physical Location & Seq

Genomic Coordinates Forward (+)

25213191 .. 25220604

Gene Structure 7414 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0261700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGACATGCTGCGTGCAGCTGTGTGTAATTTTTATCCCTCCTAGTCGGTCAACGTATAACAACTTGATCAATGCATGTGGATCTAGTGGAAATTGGAGAGAAGCTTTAAAAGTTTGCAAGAAAATGACAGATAATGGAGTTGGACCTGATCTTGTGACTCACAATATTGTTTTATCTGCATACAAAACTGGGGCTCAATATTCAAAAGCTTTATCTTATTTTGAACTTATGAAGGGAACAAATATCCGTCCAGACACAACGACCCTCAATATTGTTATATATTGCCTAGTGAAGCTTGGACAGTATGGGAAAGCCATTGATATTTTCAATTCCATGAGAGATAAGGGAGCAGAATGTCGCCCTGACATCGTAACATTCACCAGCATCATTCATTTGTATTCTGTGAGCGGGCAGATAGACAACTGCACAGCCGTATTTAATACAATTCTTGCAGAAGGGCTAAAACCTAACATTGTTTCCTATAATGCACTATTAGGTGCATATGCTTCACATGGGATGTGCACAGAGGCGGCATCAGTATTTAATGAGATGAAGAAAAGTAGTTTTCGGCCAGATGTTGTGTCTTATACATCTCTACTCAATGCTTATGGAAGATCACGACATCCTAAGAAGGCTAGGGAAGTTTTTGACAGGATGAAGGCCAACCACTTGAAGCCAAACCTTGTGAGCTACAACGCACTGATTAATGCCTATGGATCCAGTGGTTTATTAGCCGAAGCTGTTGAGGTCTTGCGTGAGATGGAGCAAGATGGAATTCATCCAAACATTGTCTCAATATGTACCCTTTTGGCTGCTTGTGGACAATGTGGCCAGAAGGTGAAAATTGATGCCATACTTTCAGCAGCCAAGCTACGAGGCATTGAATTGAACACAATTACCTATAATTCGGCTATTGGGAGCTATATGAATTTGGGAGAACATGAGAAAGCTATAAACTTGTATCAATCTATGAGAAAGAAGAAAGTGAAACCGGATTCTGTTACTTACAATGTGTTGATAAGTGGTTGTTGTAAGATGTCAAAATACAGTGAGGCACTAACCTTTTTTGATGAAATGGTGGATTTGAAAATTCCTATGTCCAAAGAAGTCTACTCATCTGTGCTCTGTGCCTACAACAAGAAGGGCCAACTTACGGAAGCGGAATCTGTATTCAACTCAATGAAGATGGCTGGATGTCCTCCTGATGTAGTTACATATACTGCAATGTTACATGCGTATAGTGCTGCAGAGAATTGGGAAAAGGCTTGTGAGCTATTTCAAGAAATGGAAATAAATGGCATCCAACCAGATGCTATTGCATGTTCAGCTTTGATGAGAGCTTTTAACAAAGGAGGCCATCCCTCTAGAGTTCTTTTTCTGGCAGAACTCATGAGAGAAAAAGAAATTCCTTTCAATGATGCCATTTTCTTTGAAATGGTTTCGGCTTGTAGCTTATTACAAGATTGGAGAACAACAATACAATTGATTAAGGTAATAGAGCCCTCATTCCCTAGACTTTCAGTCGGGCTTAAGAATCAGCTTCTGCAGATTATGGGAAGAAGCGGAAAGACAGAGGCTATGATGAAGTTGTTCTACAAGATAATTGCATCCAATGGTGATATCAACTTTGATACTTACTCAATTTTGTTGAAGAATCTTTTGTCTGTTGGAAGTTGGAGGAAATATATTGAGGTATTGCAGTGGATGGTGGATGCTAGAGTACGACCTTCAAGCCAAATGTATCTCGATATATCCTGTTTTGCACAGAAAAGTGGTGGAACTGAATATGCTTCTATTATAAATGAAAGAATCGAATTATTGAAAAGAAAAGCAAGGGAAGAAGACTCTCGAAGCAAGCTACGTGATACTCACTCCACCTCCATGCTGGTACATAGAACAAGAGCAGAGAACCACAGGCTGCTTGAATCAAGAATGGCTGTTACGCATCCTGTAGTTCCTGACTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003989 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0016020 GO:0016053 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022414 GO:0022622 GO:0030497 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044464 GO:0046394 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944 GO:0072330 GO:0090421 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00254 ko00620 ko00640 ko01100 ko01110 ko01212 ko04152 ko04910 ko04922 map00061 map00254 map00620 map00640 map01100 map01110 map01212 map04152 map04910 map04922

Pfam Domains

Protein Families

ACC_central Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2 Carboxyl_trans

Protein Analysis

655

Amino Acids

73.13

Weight (kDa)

8.78

Isoelectric Point (pI)

37.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	14 - 61	5.1e-11	PPR repeat family
TPR_24	PF23276	17 - 112	1.4e-08	Fungal tetratrico peptide repeats
PPR	PF01535	17 - 47	1.3e-07	PPR repeat
PPR_3	PF13812	17 - 61	3.6e-07	Pentatricopeptide repeat domain
PPR_long	PF17177	105 - 201	3.2e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	108 - 168	1.8e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	121 - 161	5.6e-06	PPR repeat family
PPR_1	PF12854	153 - 185	7.7e-06	PPR repeat
PPR_2	PF13041	156 - 203	3.7e-15	PPR repeat family
PPR	PF01535	159 - 188	1.4e-07	PPR repeat
PPR_long	PF17177	176 - 287	3.6e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	179 - 239	1.5e-18	Pentatricopeptide repeat domain
PPR_2	PF13041	191 - 237	6.9e-13	PPR repeat family
PPR	PF01535	194 - 222	8.4e-07	PPR repeat
TPR_24	PF23276	198 - 275	7.5e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	223 - 255	8.8e-10	PPR repeat
PPR_2	PF13041	226 - 273	2e-11	PPR repeat family
PPR	PF01535	229 - 259	1.6e-08	PPR repeat
PPR_3	PF13812	285 - 345	2.9e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	297 - 345	9e-18	PPR repeat family
PPR_long	PF17177	314 - 458	8.4e-15	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	328 - 360	2e-10	PPR repeat
PPR_2	PF13041	331 - 372	1.5e-09	PPR repeat family
PPR	PF01535	334 - 362	1.6e-08	PPR repeat
PPR_3	PF13812	356 - 413	4.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	370 - 413	1e-11	PPR repeat family
PPR_1	PF12854	398 - 430	6.9e-08	PPR repeat
PPR_2	PF13041	401 - 449	4.1e-13	PPR repeat family
PPR	PF01535	404 - 434	2.7e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001624)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53390
fragaria_vesca	FvH4_6g32451 FvH4_7g18960 FvH4_7g18960
malus_domestica	MD00G1214200.v1.1
prunus_persica	Prupe.2G210600_v2.0.a1 Prupe.2G210600_v2.0.a1 Prupe.2G210600_v2.0.a1
pyrus_communis	pycom01g13180 pycom01g13190 pycom07g16600
rosa_chinensis	RchiOBHm_Chr1g0360271 RchiOBHm_Chr1g0362891 RchiOBHm_Chr2g0140691 RchiOBHm_Chr2g0140701
rosa_laevigata	RLG00000003015 RLG00000019892 RLG00000019893 RLG00000027614
rosa_multiflora	Rmu_co8315067.1_g000001 Rmu_sc0001502.1_g000011 Rmu_sc0003342.1_g000012 Rmu_sc0003342.1_g000013
rosa_roxburghii	Rroxscaffold_1G00043370 Rroxscaffold_2G00104570 Rroxscaffold_2G00104580 Rroxscaffold_3G00227580 Rroxscaffold_4G00293670 Rroxscaffold_5G00347780 Rroxscaffold_5G00347800
rosa_rugosa	Rorug01G0302100 Rorug02G0364100 Rorug02G0364100 Rorug03G0261700
rosa_samantha	Rh1AG185000 Rh1AG311300 Rh1BG274400 Rh1DG305200 Rh2AG327100 Rh2AG415900 Rh2BG425300 Rh2BG425400 Rh2DG435400 Rh4AG110400 Rh6DG223100 Rh7BG077900
rosa_wichuraiana	Rw1G027590 Rw2G034020 Rw2G034030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1799
AccBSI	CCGCTC	1 cut(s)	408
AccI	GTMKAC	1 cut(s)	1110
AciI	CCGC	4 cut(s)	408, 530, 1160, 1563
AclWI	GGATC	3 cut(s)	88, 711, 724
AcoI	YGGCCR	2 cut(s)	569, 829
AcsI	RAATTY	4 cut(s)	774, 928, 1089, 1404
AfaI	GTAC	3 cut(s)	802, 1722, 1890
AfiI	CCNNNNNNNGG	1 cut(s)	1153
AflII	CTTAAG	1 cut(s)	1529
AhdI	GACNNNNNGTC	1 cut(s)	1520
Alw21I	GWGCWC	2 cut(s)	524, 1125
Alw26I	GTCTC	1 cut(s)	796
Alw44I	GTGCAC	1 cut(s)	520
AlwI	GGATC	3 cut(s)	88, 711, 724
AlwNI	CAGNNNCTG	1 cut(s)	1543
AoxI	GGCC	5 cut(s)	569, 660, 829, 1144, 1354
ApaLI	GTGCAC	1 cut(s)	520
ApeKI	GCWGC	6 cut(s)	9, 16, 812, 863, 1244, 1918
ApoI	RAATTY	4 cut(s)	774, 928, 1089, 1404
AseI	ATTAAT	1 cut(s)	705
Asp700I	GAANNNNTTC	1 cut(s)	1589
AspS9I	GGNCC	2 cut(s)	143, 1144
AsuHPI	GGTGA	3 cut(s)	370, 850, 1628
AvaII	GGWCC	1 cut(s)	143
BaeGI	GKGCMC	1 cut(s)	524
BalI	TGGCCA	1 cut(s)	831
BamHI	GGATCC	1 cut(s)	716
BanII	GRGCYC	2 cut(s)	196, 1503
BarI	GAAGNNNNNNTAC	2 cut(s)	1577, 1609
BbsI	GAAGAC	1 cut(s)	1848
Bbv12I	GWGCWC	2 cut(s)	524, 1125
BbvI	GCAGC	5 cut(s)	28, 799, 875, 1231, 1905
BccI	CCATC	5 cut(s)	754, 764, 1180, 1365, 1699
BceAI	ACGGC	1 cut(s)	416
BclI	TGATCA	1 cut(s)	66
BcoDI	GTCTC	1 cut(s)	796
BfaI	CTAG	7 cut(s)	42, 84, 287, 636, 1365, 1512, 1716
BfmI	CTRYAG	3 cut(s)	1245, 1544, 1950
BfrI	CTTAAG	1 cut(s)	1529
BisI	GCNGC	7 cut(s)	10, 17, 531, 813, 864, 1245, 1919
BlsI	GCNGC	7 cut(s)	11, 18, 532, 814, 865, 1246, 1920
Bme18I	GGWCC	1 cut(s)	143
BmeRI	GACNNNNNGTC	1 cut(s)	1520
BmgT120I	GGNCC	2 cut(s)	143, 1144
BmiI	GGNNCC	1 cut(s)	718
BmrI	ACTGGG	1 cut(s)	198
BmsI	GCATC	9 cut(s)	393, 542, 838, 1300, 1308, 1408, 1616, 1702, 1954
BmuI	ACTGGG	1 cut(s)	198
BpiI	GAAGAC	1 cut(s)	1848
BsaAI	YACGTR	1 cut(s)	1862
BsaWI	WCCGGW	1 cut(s)	991
BsaXI	ACNNNNNCTCC	2 cut(s)	1862, 1892
Bsc4I	CCNNNNNNNGG	1 cut(s)	1153
Bse1I	ACTGG	2 cut(s)	193, 720
Bse3DI	GCAATG	1 cut(s)	1230
BseLI	CCNNNNNNNGG	1 cut(s)	1153
BseMI	GCAATG	1 cut(s)	1230
BseNI	ACTGG	2 cut(s)	193, 720
BseSI	GKGCMC	1 cut(s)	524
BseXI	GCAGC	5 cut(s)	28, 799, 875, 1231, 1905
BsgI	GTGCAG	2 cut(s)	35, 409
BshFI	GGCC	5 cut(s)	571, 662, 831, 1146, 1356
BsiHKAI	GWGCWC	2 cut(s)	524, 1125
BsiSI	CCGG	1 cut(s)	992
BslI	CCNNNNNNNGG	1 cut(s)	1153
BsmAI	GTCTC	1 cut(s)	796
BsnI	GGCC	5 cut(s)	571, 662, 831, 1146, 1356
Bsp1286I	GDGCHC	4 cut(s)	196, 524, 1125, 1503
Bsp143I	GATC	5 cut(s)	66, 80, 148, 614, 716
BspACI	CCGC	4 cut(s)	408, 530, 1160, 1563
BspANI	GGCC	5 cut(s)	571, 662, 831, 1146, 1356
BspHI	TCATGA	1 cut(s)	1389
BspLI	GGNNCC	1 cut(s)	718
BspMAI	CTGCAG	2 cut(s)	1249, 1548
BspPI	GGATC	3 cut(s)	88, 711, 724
BspTI	CTTAAG	1 cut(s)	1529
BsrBI	CCGCTC	1 cut(s)	408
BsrDI	GCAATG	1 cut(s)	1230
BsrI	ACTGG	2 cut(s)	193, 720
BssMI	GATC	5 cut(s)	66, 80, 148, 614, 716
Bst4CI	ACNGT	2 cut(s)	303, 1049
BstAFI	CTTAAG	1 cut(s)	1529
BstBAI	YACGTR	1 cut(s)	1862
BstC8I	GCNNGC	3 cut(s)	14, 410, 1856
BstDEI	CTNAG	1 cut(s)	627
BstKTI	GATC	5 cut(s)	69, 83, 151, 617, 719
BstMAI	GTCTC	1 cut(s)	796
BstMBI	GATC	5 cut(s)	66, 80, 148, 614, 716
BstMWI	GCNNNNNNNGC	2 cut(s)	1241, 1325
BstNSI	RCATGY	4 cut(s)	9, 78, 1235, 1323
BstSFI	CTRYAG	3 cut(s)	1245, 1544, 1950
BstSLI	GKGCMC	1 cut(s)	524
BstV1I	GCAGC	5 cut(s)	28, 799, 875, 1231, 1905
BstV2I	GAAGAC	1 cut(s)	1848
BstX2I	RGATCY	2 cut(s)	80, 716
BstYI	RGATCY	2 cut(s)	80, 716
BsuRI	GGCC	5 cut(s)	571, 662, 831, 1146, 1356
BtsI	GCAGTG	1 cut(s)	1706
BtsIMutI	CAGTG	4 cut(s)	698, 727, 1054, 1706
Cac8I	GCNNGC	3 cut(s)	14, 410, 1856
CaiI	CAGNNNCTG	1 cut(s)	1543
CciI	TCATGA	1 cut(s)	1389
Cfr13I	GGNCC	2 cut(s)	143, 1144
Csp6I	GTAC	3 cut(s)	801, 1721, 1889
CspCI	CAANNNNNGTGG	2 cut(s)	58, 93
CviAII	CATG	9 cut(s)	6, 75, 334, 512, 941, 1232, 1320, 1390, 1882
CviQI	GTAC	3 cut(s)	801, 1721, 1889
DdeI	CTNAG	1 cut(s)	627
DpnI	GATC	5 cut(s)	68, 82, 150, 616, 718
DpnII	GATC	5 cut(s)	66, 80, 148, 614, 716
DraI	TTTAAA	1 cut(s)	108
DriI	GACNNNNNGTC	1 cut(s)	1520
EaeI	YGGCCR	2 cut(s)	569, 829
Eam1105I	GACNNNNNGTC	1 cut(s)	1520
Eco24I	GRGCYC	2 cut(s)	196, 1503
Eco32I	GATATC	1 cut(s)	1621
Eco47I	GGWCC	1 cut(s)	143
EcoRI	GAATTC	1 cut(s)	774
EcoRV	GATATC	1 cut(s)	1621
EcoT22I	ATGCAT	1 cut(s)	76
EcoT38I	GRGCYC	2 cut(s)	196, 1503
FaeI	CATG	9 cut(s)	9, 78, 337, 515, 944, 1235, 1323, 1393, 1885
FatI	CATG	9 cut(s)	5, 74, 333, 511, 940, 1231, 1319, 1389, 1881
FauI	CCCGC	1 cut(s)	401
FauNDI	CATATG	1 cut(s)	502
FbaI	TGATCA	1 cut(s)	66
FblI	GTMKAC	1 cut(s)	1110
Fnu4HI	GCNGC	7 cut(s)	10, 17, 531, 813, 864, 1245, 1919
FriOI	GRGCYC	2 cut(s)	196, 1503
Fsp4HI	GCNGC	7 cut(s)	10, 17, 531, 813, 864, 1245, 1919
FspBI	CTAG	7 cut(s)	42, 84, 287, 636, 1365, 1512, 1716
GluI	GCNGC	7 cut(s)	10, 17, 531, 813, 864, 1245, 1919
HaeIII	GGCC	5 cut(s)	571, 662, 831, 1146, 1356
HapII	CCGG	1 cut(s)	992
Hin1II	CATG	9 cut(s)	9, 78, 337, 515, 944, 1235, 1323, 1393, 1885
HincII	GTYRAC	1 cut(s)	51
HindII	GTYRAC	1 cut(s)	51
HindIII	AAGCTT	3 cut(s)	102, 207, 293
HinfI	GANTC	8 cut(s)	157, 995, 1163, 1534, 1654, 1809, 1844, 1925
HpaII	CCGG	1 cut(s)	992
HphI	GGTGA	3 cut(s)	370, 850, 1628
Hpy166II	GTNNAC	4 cut(s)	51, 522, 821, 1111
Hpy8I	GTNNAC	4 cut(s)	51, 522, 821, 1111
HpyAV	CCTTC	7 cut(s)	226, 449, 625, 652, 829, 1135, 1737
HpyCH4III	ACNGT	2 cut(s)	303, 1049
HpyCH4IV	ACGT	2 cut(s)	53, 1861
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1241, 1325
HpyF3I	CTNAG	1 cut(s)	627
HpySE526I	ACGT	2 cut(s)	53, 1861
Hsp92II	CATG	9 cut(s)	9, 78, 337, 515, 944, 1235, 1323, 1393, 1885
Ksp22I	TGATCA	1 cut(s)	66
Kzo9I	GATC	5 cut(s)	66, 80, 148, 614, 716
LmnI	GCTCC	3 cut(s)	347, 763, 918
Lsp1109I	GCAGC	5 cut(s)	28, 799, 875, 1231, 1905
LweI	GCATC	9 cut(s)	393, 542, 838, 1300, 1308, 1408, 1616, 1702, 1954
MaeI	CTAG	7 cut(s)	42, 84, 287, 636, 1365, 1512, 1716
MaeII	ACGT	2 cut(s)	53, 1861
MaeIII	GTNAC	6 cut(s)	154, 370, 1000, 1210, 1227, 1939
MalI	GATC	5 cut(s)	68, 82, 150, 616, 718
MbiI	CCGCTC	1 cut(s)	408
MboI	GATC	5 cut(s)	66, 80, 148, 614, 716
MboII	GAAGA	8 cut(s)	565, 624, 991, 1195, 1569, 1663, 1850, 1853
MfeI	CAATTG	1 cut(s)	1481
MflI	RGATCY	2 cut(s)	80, 716
MhlI	GDGCHC	4 cut(s)	196, 524, 1125, 1503
MlsI	TGGCCA	1 cut(s)	831
MluNI	TGGCCA	1 cut(s)	831
MlyI	GAGTC	2 cut(s)	151, 1838
MmeI	TCCRAC	4 cut(s)	121, 1327, 1648, 1655
Mox20I	TGGCCA	1 cut(s)	831
Mph1103I	ATGCAT	1 cut(s)	76
MroXI	GAANNNNTTC	1 cut(s)	1589
MscI	TGGCCA	1 cut(s)	831
MseI	TTAA	7 cut(s)	107, 438, 543, 705, 1344, 1488, 1530
MslI	CAYNNNNRTG	1 cut(s)	1880
Msp20I	TGGCCA	1 cut(s)	831
MspA1I	CMGCKG	1 cut(s)	19
MspCI	CTTAAG	1 cut(s)	1529
MspI	CCGG	1 cut(s)	992
MunI	CAATTG	1 cut(s)	1481
MwoI	GCNNNNNNNGC	2 cut(s)	1241, 1325
NdeI	CATATG	1 cut(s)	502
NdeII	GATC	5 cut(s)	66, 80, 148, 614, 716
NlaIII	CATG	9 cut(s)	9, 78, 337, 515, 944, 1235, 1323, 1393, 1885
NlaIV	GGNNCC	1 cut(s)	718
NmuCI	GTSAC	1 cut(s)	154
NsiI	ATGCAT	1 cut(s)	76
NspI	RCATGY	4 cut(s)	9, 78, 1235, 1323
PagI	TCATGA	1 cut(s)	1389
PdmI	GAANNNNTTC	1 cut(s)	1589
PfeI	GAWTC	6 cut(s)	995, 1163, 1534, 1654, 1809, 1925
PkrI	GCNGC	7 cut(s)	11, 18, 532, 814, 865, 1246, 1920
PleI	GAGTC	2 cut(s)	151, 1838
PpsI	GAGTC	2 cut(s)	151, 1838
Ppu21I	YACGTR	1 cut(s)	1862
PshBI	ATTAAT	1 cut(s)	705
PsiI	TTATAA	1 cut(s)	1799
PspN4I	GGNNCC	1 cut(s)	718
PspPI	GGNCC	2 cut(s)	143, 1144
PstI	CTGCAG	2 cut(s)	1249, 1548
PstNI	CAGNNNCTG	1 cut(s)	1543
PsuI	RGATCY	2 cut(s)	80, 716
PvuII	CAGCTG	1 cut(s)	19
RsaI	GTAC	3 cut(s)	802, 1722, 1890
RsaNI	GTAC	3 cut(s)	801, 1721, 1889
RseI	CAYNNNNRTG	1 cut(s)	1880
SaqAI	TTAA	7 cut(s)	107, 438, 543, 705, 1344, 1488, 1530
SatI	GCNGC	7 cut(s)	10, 17, 531, 813, 864, 1245, 1919
Sau3AI	GATC	5 cut(s)	66, 80, 148, 614, 716
Sau96I	GGNCC	2 cut(s)	143, 1144
SchI	GAGTC	2 cut(s)	151, 1838
SduI	GDGCHC	4 cut(s)	196, 524, 1125, 1503
SfaNI	GCATC	9 cut(s)	393, 542, 838, 1300, 1308, 1408, 1616, 1702, 1954
SfcI	CTRYAG	3 cut(s)	1245, 1544, 1950
SinI	GGWCC	1 cut(s)	143
SmiMI	CAYNNNNRTG	1 cut(s)	1880
SmlI	CTYRAG	1 cut(s)	1529
SmoI	CTYRAG	1 cut(s)	1529
SsiI	CCGC	4 cut(s)	408, 530, 1160, 1563
SspI	AATATT	3 cut(s)	166, 200, 271
SspMI	CTAG	7 cut(s)	42, 84, 287, 636, 1365, 1512, 1716
TaaI	ACNGT	2 cut(s)	303, 1049
TaiI	ACGT	2 cut(s)	56, 1864
TaqI	TCGA	3 cut(s)	1746, 1812, 1849
TaqII	GACCGA	1 cut(s)	36
TauI	GCSGC	1 cut(s)	533
TfiI	GAWTC	6 cut(s)	995, 1163, 1534, 1654, 1809, 1925
Tru1I	TTAA	7 cut(s)	107, 438, 543, 705, 1344, 1488, 1530
Tru9I	TTAA	7 cut(s)	107, 438, 543, 705, 1344, 1488, 1530
TscAI	CASTG	4 cut(s)	705, 727, 1054, 1706
TseFI	GTSAC	1 cut(s)	154
TseI	GCWGC	6 cut(s)	9, 16, 812, 863, 1244, 1918
Tsp45I	GTSAC	1 cut(s)	154
TspGWI	ACGGA	2 cut(s)	236, 1169
TspRI	CASTG	4 cut(s)	705, 727, 1054, 1706
Vha464I	CTTAAG	1 cut(s)	1529
VneI	GTGCAC	1 cut(s)	520
VpaK11BI	GGWCC	1 cut(s)	143
VspI	ATTAAT	1 cut(s)	705
XapI	RAATTY	4 cut(s)	774, 928, 1089, 1404
XbaI	TCTAGA	1 cut(s)	1364
XceI	RCATGY	4 cut(s)	9, 78, 1235, 1323
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1882
XmiI	GTMKAC	1 cut(s)	1110
XmnI	GAANNNNTTC	1 cut(s)	1589
XspI	CTAG	7 cut(s)	42, 84, 287, 636, 1365, 1512, 1716
Zsp2I	ATGCAT	1 cut(s)	76

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.