Rh1DG304900

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0018477
Physical Location & Seq
Reverse (-)
53222862 .. 53224178
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG304900.1

Sequence Viewer

Length: 894 bp
ATGGATCCCTACACGCAACTCACCAGTACACACGGCCTCAGAGTCTTCCAACTCTCAGAGCTCGAGAAGGCCACCAAGAACTTCAGCTCGTCTCGCTGCGTTGGAGGGGGAGATTTTGGAGCTGAGATCTTTAAGGGAGTGATCAAGAATGCCAACAAAACAAAACTTCATGTACTGGTCTATAGGATCACCCGAGGACCTACTCTTGAGGCAAAAAGCGACGAAGAGTGGGCGAGGGAGCTCACAAAACAAGTATCAGCTATTGATGATCATCCAAACTTTGTTAAAGTGGTGGGATATTGTGCCACTCGATACAAGAAGAAGAAGGGACATGTAGAGCCTGTACGATTTATAGTGGAAGAAGATGCATGCAATGGAAATTTACATGACTATCTACATGAGGGGCGGCATCAACCTCTTTCATGGGCAAAGAGGCTCAGCATACTCCGAGATGTTGCTCGTGGTTTGGCTTATTTTCATGATTCATTAGATAACAAGATACTATCTTTTAAGTCCTCCAACATACTCTTGGATGAGAACGACGTTACATGTGTTTCTGTGAGTTATGAAGCTCCTGAACTCCTTCTAAGAGGCGACTTGACGTCCAAGAGCAACGTGTGGAGCTATGGCGTCTTTATCTTGGAGGTCATAACTGGTAACCAGCCATCTGATGGAAACCTGACGAAAAAGTTAAAGAAATTATGGAGCTCCGATAGAAACAAGTTGGAGAAGATGATAGACCCCAAGCTTAATGGAAGGTATTCTGCAGAATCGGCAATGGCACTAGCAGCACTTGCAGCTGATCATTGCTTGCAGGAAGAGCCAAACAAGCGACCTAAAATGAGTGAGGTCCTTGCAATGGTGTCTACAATGGCAGAGACAGCAGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

297

Amino Acids

33.3

Weight (kDa)

8.16

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 31 - 182 4.4e-11 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 180 - 286 8.3e-11 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 183 - 285 4.4e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018477)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18940
rosa_chinensis RchiOBHm_Chr1g0362871
rosa_multiflora Rmu_sc0001502.1_g000013
rosa_roxburghii Rroxscaffold_4G00293690
rosa_rugosa Rorug01G0302100
rosa_samantha Rh1AG311000 Rh1BG274100 Rh1CG291300 Rh1DG304900
rosa_wichuraiana Rw1G027570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 605
AccB7I CCANNNNNTGG 1 cut(s) 671
AccI GTMKAC 1 cut(s) 866
AciI CCGC 1 cut(s) 406
AclWI GGATC 2 cut(s) 12, 194
AcsI RAATTY 2 cut(s) 379, 888
AcuI CTGAAG 1 cut(s) 67
AcyI GRCGYC 2 cut(s) 602, 630
AfaI GTAC 3 cut(s) 28, 174, 345
AfiI CCNNNNNNNGG 2 cut(s) 671, 859
AflIII ACRYGT 3 cut(s) 331, 548, 615
AhdI GACNNNNNGTC 1 cut(s) 601
Alw21I GWGCWC 3 cut(s) 63, 243, 710
Alw26I GTCTC 2 cut(s) 96, 872
AlwI GGATC 2 cut(s) 12, 194
Ama87I CYCGRG 2 cut(s) 62, 192
AoxI GGCC 2 cut(s) 34, 69
ApeKI GCWGC 3 cut(s) 96, 788, 797
ApoI RAATTY 2 cut(s) 379, 888
Asp700I GAANNNNTTC 2 cut(s) 582, 760
AspS9I GGNCC 2 cut(s) 197, 850
AsuHPI GGTGA 2 cut(s) 13, 181
AvaI CYCGRG 2 cut(s) 62, 192
AvaII GGWCC 2 cut(s) 197, 850
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 63, 243, 710
BauI CACGAG 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 3 cut(s) 63, 243, 710
BbvI GCAGC 3 cut(s) 83, 800, 809
BccI CCATC 2 cut(s) 665, 673
BceAI ACGGC 1 cut(s) 49
BclI TGATCA 3 cut(s) 141, 268, 802
BcoDI GTCTC 2 cut(s) 96, 872
BfaI CTAG 1 cut(s) 785
BfmI CTRYAG 2 cut(s) 181, 765
BglII AGATCT 1 cut(s) 126
BisI GCNGC 4 cut(s) 97, 407, 789, 798
BlpI GCTNAGC 1 cut(s) 437
BlsI GCNGC 4 cut(s) 98, 408, 790, 799
Bme18I GGWCC 2 cut(s) 197, 850
BmeRI GACNNNNNGTC 1 cut(s) 601
BmeT110I CYCGRG 2 cut(s) 62, 192
BmgT120I GGNCC 2 cut(s) 197, 850
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 2 cut(s) 355, 418
BpiI GAAGAC 1 cut(s) 37
Bpu1102I GCTNAGC 1 cut(s) 437
BpuEI CTTGAG 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 270
BsaHI GRCGYC 2 cut(s) 602, 630
BsaJI CCNNGG 1 cut(s) 193
Bsc4I CCNNNNNNNGG 2 cut(s) 671, 859
Bse1I ACTGG 3 cut(s) 24, 180, 658
Bse3DI GCAATG 4 cut(s) 379, 783, 805, 864
Bse8I GATNNNNATC 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 2 cut(s) 271, 538
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 2 cut(s) 671, 859
BseMI GCAATG 4 cut(s) 379, 783, 805, 864
BseMII CTCAG 4 cut(s) 52, 69, 114, 451
BseNI ACTGG 3 cut(s) 24, 180, 658
BseXI GCAGC 3 cut(s) 83, 800, 809
BshFI GGCC 2 cut(s) 36, 71
BsiHKAI GWGCWC 3 cut(s) 63, 243, 710
BsiHKCI CYCGRG 2 cut(s) 62, 192
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 2 cut(s) 671, 859
BsmAI GTCTC 2 cut(s) 96, 872
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 342
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 2 cut(s) 36, 71
BsoBI CYCGRG 2 cut(s) 62, 192
Bsp1286I GDGCHC 3 cut(s) 63, 243, 710
Bsp143I GATC 6 cut(s) 4, 126, 141, 186, 268, 802
Bsp1720I GCTNAGC 1 cut(s) 437
BspACI CCGC 1 cut(s) 406
BspANI GGCC 2 cut(s) 36, 71
BspCNI CTCAG 4 cut(s) 51, 68, 115, 450
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 769
BspPI GGATC 2 cut(s) 12, 194
BspQI GCTCTTC 1 cut(s) 813
BsrDI GCAATG 4 cut(s) 379, 783, 805, 864
BsrI ACTGG 3 cut(s) 24, 180, 658
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 6 cut(s) 4, 126, 141, 186, 268, 802
BssNI GRCGYC 2 cut(s) 602, 630
BssSI CACGAG 1 cut(s) 459
Bst2BI CACGAG 1 cut(s) 459
Bst6I CTCTTC 2 cut(s) 219, 813
BstACI GRCGYC 2 cut(s) 602, 630
BstAPI GCANNNNNTGC 1 cut(s) 794
BstC8I GCNNGC 2 cut(s) 370, 812
BstDEI CTNAG 5 cut(s) 38, 55, 123, 437, 587
BstEII GGTNACC 1 cut(s) 656
BstF5I GGATG 2 cut(s) 271, 538
BstKTI GATC 6 cut(s) 7, 129, 144, 189, 271, 805
BstMAI GTCTC 2 cut(s) 96, 872
BstMBI GATC 6 cut(s) 4, 126, 141, 186, 268, 802
BstMWI GCNNNNNNNGC 8 cut(s) 93, 773, 788, 794, 797, 820, 829, 881
BstNSI RCATGY 3 cut(s) 335, 372, 552
BstPI GGTNACC 1 cut(s) 656
BstSFI CTRYAG 2 cut(s) 181, 765
BstV1I GCAGC 3 cut(s) 83, 800, 809
BstV2I GAAGAC 1 cut(s) 37
BstX2I RGATCY 2 cut(s) 4, 126
BstYI RGATCY 2 cut(s) 4, 126
BsuRI GGCC 2 cut(s) 36, 71
BtsCI GGATG 2 cut(s) 271, 538
Cac8I GCNNGC 2 cut(s) 370, 812
CciI TCATGA 1 cut(s) 478
Cfr13I GGNCC 2 cut(s) 197, 850
CseI GACGC 1 cut(s) 619
Csp6I GTAC 3 cut(s) 27, 173, 344
CviAII CATG 8 cut(s) 170, 332, 369, 386, 398, 423, 479, 549
CviQI GTAC 3 cut(s) 27, 173, 344
DdeI CTNAG 5 cut(s) 38, 55, 123, 437, 587
DpnI GATC 6 cut(s) 6, 128, 143, 188, 270, 804
DpnII GATC 6 cut(s) 4, 126, 141, 186, 268, 802
DriI GACNNNNNGTC 1 cut(s) 601
Eam1104I CTCTTC 2 cut(s) 219, 813
Eam1105I GACNNNNNGTC 1 cut(s) 601
EarI CTCTTC 2 cut(s) 219, 813
Ecl136II GAGCTC 3 cut(s) 61, 241, 708
Eco24I GRGCYC 3 cut(s) 63, 243, 710
Eco47I GGWCC 2 cut(s) 197, 850
Eco53kI GAGCTC 3 cut(s) 61, 241, 708
Eco57I CTGAAG 1 cut(s) 67
Eco88I CYCGRG 2 cut(s) 62, 192
Eco91I GGTNACC 1 cut(s) 656
EcoICRI GAGCTC 3 cut(s) 61, 241, 708
EcoO109I RGGNCCY 2 cut(s) 197, 850
EcoO65I GGTNACC 1 cut(s) 656
EcoT22I ATGCAT 1 cut(s) 370
EcoT38I GRGCYC 3 cut(s) 63, 243, 710
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 8 cut(s) 173, 335, 372, 389, 401, 426, 482, 552
FaqI GGGAC 1 cut(s) 342
FatI CATG 8 cut(s) 169, 331, 368, 385, 397, 422, 478, 548
FbaI TGATCA 3 cut(s) 141, 268, 802
FblI GTMKAC 1 cut(s) 866
Fnu4HI GCNGC 4 cut(s) 97, 407, 789, 798
FokI GGATG 2 cut(s) 258, 545
FriOI GRGCYC 3 cut(s) 63, 243, 710
Fsp4HI GCNGC 4 cut(s) 97, 407, 789, 798
FspBI CTAG 1 cut(s) 785
GluI GCNGC 4 cut(s) 97, 407, 789, 798
HaeIII GGCC 2 cut(s) 36, 71
HgaI GACGC 1 cut(s) 619
Hin1I GRCGYC 2 cut(s) 602, 630
Hin1II CATG 8 cut(s) 173, 335, 372, 389, 401, 426, 482, 552
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 3 cut(s) 42, 482, 770
HphI GGTGA 2 cut(s) 13, 181
Hpy166II GTNNAC 2 cut(s) 29, 867
Hpy188I TCNGA 5 cut(s) 41, 58, 449, 670, 712
Hpy188III TCNNGA 5 cut(s) 64, 145, 206, 479, 575
Hpy8I GTNNAC 2 cut(s) 29, 867
Hpy99I CGWCG 2 cut(s) 224, 545
HpyAV CCTTC 4 cut(s) 61, 319, 593, 750
HpyCH4IV ACGT 3 cut(s) 543, 602, 615
HpyCH4V TGCA 6 cut(s) 368, 372, 767, 797, 814, 857
HpyF10VI GCNNNNNNNGC 8 cut(s) 93, 773, 788, 794, 797, 820, 829, 881
HpyF3I CTNAG 5 cut(s) 38, 55, 123, 437, 587
HpySE526I ACGT 3 cut(s) 543, 602, 615
Hsp92I GRCGYC 2 cut(s) 602, 630
Hsp92II CATG 8 cut(s) 173, 335, 372, 389, 401, 426, 482, 552
Ksp22I TGATCA 3 cut(s) 141, 268, 802
Kzo9I GATC 6 cut(s) 4, 126, 141, 186, 268, 802
LguI GCTCTTC 1 cut(s) 813
LmnI GCTCC 6 cut(s) 119, 238, 577, 621, 705, 713
LpnPI CCDG 8 cut(s) 37, 161, 354, 588, 639, 674, 692, 800
Lsp1109I GCAGC 3 cut(s) 83, 800, 809
LweI GCATC 2 cut(s) 355, 418
MaeI CTAG 1 cut(s) 785
MaeII ACGT 3 cut(s) 543, 602, 615
MaeIII GTNAC 2 cut(s) 544, 656
MalI GATC 6 cut(s) 6, 128, 143, 188, 270, 804
MboI GATC 6 cut(s) 4, 126, 141, 186, 268, 802
MboII GAAGA 8 cut(s) 37, 236, 331, 334, 371, 374, 742, 830
MflI RGATCY 2 cut(s) 4, 126
MhlI GDGCHC 3 cut(s) 63, 243, 710
MluCI AATT 3 cut(s) 379, 698, 888
MlyI GAGTC 1 cut(s) 51
MmeI TCCRAC 4 cut(s) 73, 82, 543, 705
Mph1103I ATGCAT 1 cut(s) 370
MroXI GAANNNNTTC 2 cut(s) 582, 760
MseI TTAA 5 cut(s) 132, 285, 510, 692, 750
MspA1I CMGCKG 1 cut(s) 800
Mva1269I GAATGC 1 cut(s) 154
MwoI GCNNNNNNNGC 8 cut(s) 93, 773, 788, 794, 797, 820, 829, 881
NdeII GATC 6 cut(s) 4, 126, 141, 186, 268, 802
NlaIII CATG 8 cut(s) 173, 335, 372, 389, 401, 426, 482, 552
NlaIV GGNNCC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 3 cut(s) 335, 372, 552
PaeI GCATGC 1 cut(s) 372
PaeR7I CTCGAG 1 cut(s) 62
PagI TCATGA 1 cut(s) 478
PciI ACATGT 2 cut(s) 331, 548
PciSI GCTCTTC 1 cut(s) 813
PctI GAATGC 1 cut(s) 154
PdmI GAANNNNTTC 2 cut(s) 582, 760
PfeI GAWTC 2 cut(s) 482, 770
PflMI CCANNNNNTGG 1 cut(s) 671
PkrI GCNGC 4 cut(s) 98, 408, 790, 799
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PpuMI RGGWCCY 2 cut(s) 197, 850
PscI ACATGT 2 cut(s) 331, 548
Psp124BI GAGCTC 3 cut(s) 63, 243, 710
Psp5II RGGWCCY 2 cut(s) 197, 850
PspEI GGTNACC 1 cut(s) 656
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 197, 850
PspPPI RGGWCCY 2 cut(s) 197, 850
PstI CTGCAG 1 cut(s) 769
PsuI RGATCY 2 cut(s) 4, 126
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 3 cut(s) 28, 174, 345
RsaNI GTAC 3 cut(s) 27, 173, 344
SacI GAGCTC 3 cut(s) 63, 243, 710
SapI GCTCTTC 1 cut(s) 813
SaqAI TTAA 5 cut(s) 132, 285, 510, 692, 750
SatI GCNGC 4 cut(s) 97, 407, 789, 798
Sau3AI GATC 6 cut(s) 4, 126, 141, 186, 268, 802
Sau96I GGNCC 2 cut(s) 197, 850
SchI GAGTC 1 cut(s) 51
SduI GDGCHC 3 cut(s) 63, 243, 710
SfaNI GCATC 2 cut(s) 355, 418
SfcI CTRYAG 2 cut(s) 181, 765
Sfr274I CTCGAG 1 cut(s) 62
SinI GGWCC 2 cut(s) 197, 850
SlaI CTCGAG 1 cut(s) 62
SmlI CTYRAG 2 cut(s) 62, 206
SmoI CTYRAG 2 cut(s) 62, 206
SphI GCATGC 1 cut(s) 372
Sse9I AATT 3 cut(s) 379, 698, 888
SsiI CCGC 1 cut(s) 406
SspMI CTAG 1 cut(s) 785
SstI GAGCTC 3 cut(s) 63, 243, 710
TaiI ACGT 3 cut(s) 546, 605, 618
TaqI TCGA 2 cut(s) 63, 310
TasI AATT 3 cut(s) 379, 698, 888
TatI WGTACW 2 cut(s) 26, 172
TauI GCSGC 1 cut(s) 409
TfiI GAWTC 2 cut(s) 482, 770
Tru1I TTAA 5 cut(s) 132, 285, 510, 692, 750
Tru9I TTAA 5 cut(s) 132, 285, 510, 692, 750
TseI GCWGC 3 cut(s) 96, 788, 797
TspDTI ATGAA 5 cut(s) 158, 411, 467, 474, 582
Van91I CCANNNNNTGG 1 cut(s) 671
VpaK11BI GGWCC 2 cut(s) 197, 850
XapI RAATTY 2 cut(s) 379, 888
XceI RCATGY 3 cut(s) 335, 372, 552
XcmI CCANNNNNNNNNTGG 2 cut(s) 526, 668
XhoI CTCGAG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 866
XmnI GAANNNNTTC 2 cut(s) 582, 760
XspI CTAG 1 cut(s) 785
ZraI GACGTC 1 cut(s) 603
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.