Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0010245

Physical Location & Seq

Genomic Coordinates Reverse (-)

28515627 .. 28534184

Gene Structure 18558 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00307330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3288 bp

ATGGCAATCCTCGCTCTCCCGCTAGTCATCTCCGCTCTCAAGCCTCACGCTTCCCGCCTCTACCTCCTCCGCCGCGCCGCCCCTTCTTCCCGCCTCCGCAACCCACTCAATTACTACCACCGCCACTTCACCAAAACCGCCGTCTCTGCTATCAGCACCACCTCTGCCGTTCCTCACCAGTCCTCTACTGACCCACTTATTGAGCCCAGCAAAGCTTCAGTCCTCACTTTCCAACAAGCCATTCAGCGCCTCCAGGAATATTGGGCTTCAGTTGGGTGCGCCATTATGCAATGCAGCAACACAGAGGTTGGAGCTGGGACTATGAATCCCTTGACTTTCCTGAGGGTGCTTGGTCCAGAACCGTGGAATGTAGCGTATCCGGAGCCTAGTATCCGACCAGATGATAGTCGTTACGGGGAAAACCCAAATAGGCTCCAGAGACACACTCAATTTCAGGTTATATTGAAGCCTGATCCTGGAAACTCACAAGACCTTTTTATCCGCAGCTTATCAGCTTTAGGTATTGATGTTTGTTCTCATGATATACGATTTGTGGAGGACAACTGGGAGAGTCCGGTGCTTGGAGCTTGGGGTTTGGGTTGGGAAATCTGGATGGATGGGATGGAAATTACACAGTTTACCTACTTTCAGCAGGCTGGAAGTCTTCAATTGTCACCAGTATCTGTTGAAATCACCTATGGTCTTGAGCGAATCCTCATGTTGCTTCAGGGAGTTGATCATTTTAAGAAAATTCAATATGCTGATGGAATTACATATGGGGAGCTATTCTTGGAGAATGAGAAGGAAATGAGTGCATACTATCTAGAGCATGCTGGTGTAAATCATATTCAAAAACAGTTTGATCTTTCTGAGGAGGAAGCTCGTTCCTTGCTTGCTTCAGGCCTAGCAATACCTGCGTATGATCAACTTCTGAAAACATCACATGCTTTCAATATATTAGACTCTAGAGGCTTTGTTGGGGTAACAGAGCGTGCTCGTTACTTTGGTCGGATGCGTAGTCCCATATTTTTCAAATCATCTGTGATTGCACACATCCTTTTAGCTCGTCAGTGTGCACAACTTTGGTTAAAGACCAGGGAGTCCCTTGGTTATCCATTGGGTGTAGTTTCTGAAACTGCTAATCTTGTATGTCCTAAAGAGCTTGTGGAGGCAGCGGTCAAGAAGGTGCATGATACTGCAAGGTCATTTGTTCTCGAAATTGGAATTGAAGAGATGCCACCTGAAGACGTAGTTGATGCAAGCCAGCAACTGAAAGATCTAATAGTGCAGTTACTAGCAAAGCAAAGACTGGGCCATGGTGAAGTGCAAGCTTTTGGCACACCTCGCAGACTTGTGGTTTGTGTTGAGAATCTCTGCACTAAACAAATGGAGAATGAGGTTGAGGTTCGAGGGCCTCCTGTTTCAAAATCATTTGATGATCAAGGAAATCCTACAAAGGCTGCCGAGGGTTTCTGCCGGAGATACTCTGTACCATTGAACTCATTGTATCGAAAGACTGATGGGAAAACAGAATATATTTATGCTCGTGTAATAGAGTCTGCTCGACTTGCTTTGGAGGTGTTGGCTGAGGATTTGCCCAATTCGATTGCTAGAATATCATTTCCAAAATCAATGCGCTGGAACTCTCAGGTTTTGTTTAGCAGACCTATTCGTTGGATTTTAGCACTCCATGGAGATGTAGTTGTGCCCTTTACTTTTGCTCAAGTTTTAAGTGGAAACCTGTCTTATGGTCTTCGTAACACTCCTTCCGCTACTGTCACGGTTGAAACTGCGGAATCTTATGCTGGTGTAATGAGGAATGCGGGGATCAATATTGAAATGGAGGTTGTAAATCTTGTTGAGGCACCTGTTCCAGTCCTTGGGGAGTTTAAAAGGTCCTTCTTAGAGCTTCCGAGTGATCTTCTGACTATGTCCTTTGTGTCTTTTGGCCTTCCAGACTATATTGTTCCTTTGTTGCCTGTTAATCAAAGCTTTGCAAACGATGGGGAATTTTTCATTAGGATGCTTGTCATTGAGAAGTCTTTCCAAGCAAAGGTTATGCAGAAGCATCAGAAGTACTTTTCTGTGAGGGATGAGAATGGAGGGCTGTTGCCTTACTTCATTGCTGTGGCAAATGGGGCAATTGACGAGATGGTGGTTAGAAAAGGAAATGAAGCTGTATTGAGAGCTCGCTATGAAGATGCTAAATTTTTCTATGAGATGGATACACGGAAAAGGTTTTCAGAATTTAGAAGTCAACTGAAAGGGATTCTTTTCCATGAGAAGTTAGGAACAATGCTGGAGAAGGTGCTGCGTCTTGAGAAAATGGTTGACAAACTGACTTTGGCCCTTGGAATGGATGACAGTACGAATAAAATTGCCCAGCATGCTGCATCCCTTGCTATGTCAGATCTTGCTACAGCAGTTGTCACAGAATTTACGTCTCTTTCAGGAGTAATGGCTCGTCATTATGCTCTAAGAGATGGCCATTCAGAGCAGGTTGCAGAGGCTTTATTCGAGATTACACTTCCTAGATTTTCTGGAGATATACTTCCTAAAACTGATGCAGGCATAGTTCTGTCAGTAGCCGACAGATTAGATAGCCTTGTCGGTTTGTTTGCTGCTGGTTGTCAGCCCAGTTCTACTAATGACCCATTTGGCCTGCGACGAATCTCTTATGGTCTTGTCCAGGTGTTGGTGGAAAAAGATAAGTATCTGGATTTACAACAAGCTTTGGAACTTGCAGCAGATGTGCAACCCATGAAAGTAGATGCCCCTACTGTAAAAGATGCACATCAATTCATTACTCGAAGATTAGAACAATATCTGGTTGATAAGGGAATAAGTTCAGAAGTGGTTCGATCAGTCCTTGCAGAGCGTGCAAACTTACCTTGTCTAGCAGCAAGGACTGCTTCTAAAATGGAAGCCTTGTCGAAAGGCGAGCTTTTGCCAAAGGTTCTTGAAGCATATTCTCGTCCTACGAGAATTGTCCGAGGAAAGGATGTGGACCCTCATATTGAGGTGGATGAGGCAGCCTTTGAGACAGACGAAGAGAGAGCTCTATGGATTTCATTCTTGTCGGTTAAAAACAAAATATGCCATGGTATTGAGGTTGACGAGTTTGTCAAAATATCAGCACAGCTAGTACAACCACTTGACGATTTCTTTAATCATGTGTTTGTTATGGTGGAAGATGAAAGAATTCGAAATAACAGGCTTGCTTTGCTGAAGAAAATTGCTGACCTTCCAAGAGGAGTAGCGGACCTATCAATGTTGCCCGGATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004820 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006426 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045995 GO:0046483 GO:0046983 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0061458 GO:0065007 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:2000026

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2e tRNA_synt_2f

Protein Analysis

1095

Amino Acids

122.57

Weight (kDa)

5.94

Isoelectric Point (pI)

43.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2e	PF02091	77 - 340	2.9e-125	Glycyl-tRNA synthetase alpha subunit
tRNA_synt_2f	PF02092	403 - 613	2.5e-59	Glycyl-tRNA synthetase beta subunit
tRNA_synt_2f	PF02092	683 - 958	2.9e-92	Glycyl-tRNA synthetase beta subunit
DALR_1	PF05746	986 - 1080	5.2e-06	DALR anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010245)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48110
fragaria_vesca	FvH4_7g10270 FvH4_7g10270 FvH4_7g10270 FvH4_7g10270
malus_domestica	MD07G1109400.v1.1
prunus_persica	Prupe.2G132400_v2.0.a1 Prupe.2G132400_v2.0.a1 Prupe.2G132400_v2.0.a1
pyrus_communis	pycom07g09370 pycom07g09390
rosa_chinensis	RchiOBHm_Chr1g0348021
rosa_laevigata	RLG00000028699
rosa_multiflora	Rmu_sc0018061.1_g000002
rosa_roxburghii	Rroxscaffold_4G00307330
rosa_rugosa	Rorug01G0190600
rosa_samantha	Rh1AG209200 Rh1BG174200 Rh1CG193600 Rh1DG204000
rosa_wichuraiana	Rw1G017430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1099, 3122
Acc36I	ACCTGC	2 cut(s)	922, 2488
AccB1I	GGYRCC	1 cut(s)	1864
AccB7I	CCANNNNNTGG	2 cut(s)	1880, 2695
AccBSI	CCGCTC	1 cut(s)	35
AccII	CGCG	1 cut(s)	75
AccIII	TCCGGA	1 cut(s)	379
AclWI	GGATC	2 cut(s)	467, 1835
AcoI	YGGCCR	1 cut(s)	2485
AcsI	RAATTY	6 cut(s)	750, 2009, 2207, 2246, 2435, 3201
AcuI	CTGAAG	6 cut(s)	201, 252, 710, 882, 1263, 3248
AfaI	GTAC	4 cut(s)	1491, 2078, 2368, 3147
AfiI	CCNNNNNNNGG	7 cut(s)	476, 581, 1880, 2053, 2356, 2695, 2998
AjnI	CCWGG	4 cut(s)	252, 475, 1094, 2688
AjuI	GAANNNNNNNTTGG	2 cut(s)	225, 257
Alw21I	GWGCWC	4 cut(s)	997, 1078, 2191, 3061
Alw26I	GTCTC	4 cut(s)	148, 433, 2448, 3035
Alw44I	GTGCAC	1 cut(s)	1074
AlwI	GGATC	2 cut(s)	467, 1835
AlwNI	CAGNNNCTG	2 cut(s)	683, 1270
Aor13HI	TCCGGA	1 cut(s)	379
AoxI	GGCC	7 cut(s)	901, 1312, 1412, 1948, 2346, 2485, 2659
ApaLI	GTGCAC	1 cut(s)	1074
ApoI	RAATTY	6 cut(s)	750, 2009, 2207, 2246, 2435, 3201
ArsI	GACNNNNNNTTYG	6 cut(s)	126, 158, 204, 236, 1341, 1373
Asp700I	GAANNNNTTC	4 cut(s)	2042, 2845, 2856, 3201
AspLEI	GCGC	4 cut(s)	77, 249, 281, 1638
AspS9I	GGNCC	7 cut(s)	353, 1312, 1412, 1896, 2347, 3007, 3262
AsuC2I	CCSGG	1 cut(s)	3279
AsuHPI	GGTGA	5 cut(s)	121, 167, 666, 685, 1331
AsuII	TTCGAA	1 cut(s)	3205
AvaII	GGWCC	4 cut(s)	353, 1896, 3007, 3262
AxyI	CCTNAGG	1 cut(s)	341
BaeGI	GKGCMC	2 cut(s)	1078, 1710
BalI	TGGCCA	1 cut(s)	2487
BanI	GGYRCC	1 cut(s)	1864
BanII	GRGCYC	3 cut(s)	207, 2191, 3061
BarI	GAAGNNNNNNTAC	2 cut(s)	1750, 1782
BauI	CACGAG	1 cut(s)	1545
BbsI	GAAGAC	3 cut(s)	656, 1251, 1745
Bbv12I	GWGCWC	4 cut(s)	997, 1078, 2191, 3061
BbvCI	CCTCAGC	1 cut(s)	1587
BccI	CCATC	9 cut(s)	607, 611, 616, 758, 1514, 1997, 2146, 2215, 2477
BceAI	ACGGC	2 cut(s)	125, 152
BciT130I	CCWGG	4 cut(s)	254, 477, 1096, 2690
BciVI	GTATCC	3 cut(s)	387, 401, 2218
BclI	TGATCA	3 cut(s)	736, 922, 1438
BcnI	CCSGG	1 cut(s)	3279
BcoDI	GTCTC	4 cut(s)	148, 433, 2448, 3035
BfmI	CTRYAG	1 cut(s)	2418
BfoI	RGCGCY	1 cut(s)	250
BfuAI	ACCTGC	2 cut(s)	922, 2488
BfuI	GTATCC	3 cut(s)	387, 401, 2218
BglII	AGATCT	2 cut(s)	1276, 2410
BmcAI	AGTACT	1 cut(s)	2078
Bme1390I	CCNGG	5 cut(s)	254, 477, 1096, 2690, 3279
Bme18I	GGWCC	4 cut(s)	353, 1896, 3007, 3262
BmgT120I	GGNCC	7 cut(s)	353, 1312, 1412, 1896, 2347, 3007, 3262
BmiI	GGNNCC	4 cut(s)	384, 434, 1866, 3009
BmrFI	CCNGG	5 cut(s)	254, 477, 1096, 2690, 3279
BmrI	ACTGGG	3 cut(s)	574, 1319, 2631
BmuI	ACTGGG	3 cut(s)	574, 1319, 2631
BpiI	GAAGAC	3 cut(s)	656, 1251, 1745
BplI	GAGNNNNNCTC	2 cut(s)	430, 462
BpmI	CTGGAG	4 cut(s)	236, 419, 2321, 2562
Bpu10I	CCTNAGC	1 cut(s)	1587
Bpu14I	TTCGAA	1 cut(s)	3205
BpuEI	CTTGAG	4 cut(s)	23, 725, 1707, 2339
BpuMI	CCSGG	1 cut(s)	3279
BsaBI	GATNNNNATC	2 cut(s)	2712, 2793
BsaWI	WCCGGW	2 cut(s)	379, 574
BsaXI	ACNNNNNCTCC	2 cut(s)	1686, 1716
Bsc4I	CCNNNNNNNGG	7 cut(s)	476, 581, 1880, 2053, 2356, 2695, 2998
Bse1I	ACTGG	6 cut(s)	178, 569, 677, 1314, 1874, 2637
Bse21I	CCTNAGG	1 cut(s)	341
Bse3DI	GCAATG	2 cut(s)	296, 2121
Bse8I	GATNNNNATC	2 cut(s)	2712, 2793
BseAI	TCCGGA	1 cut(s)	379
BseBI	CCWGG	4 cut(s)	254, 477, 1096, 2690
BseJI	GATNNNNATC	2 cut(s)	2712, 2793
BseLI	CCNNNNNNNGG	7 cut(s)	476, 581, 1880, 2053, 2356, 2695, 2998
BseMI	GCAATG	2 cut(s)	296, 2121
BseMII	CTCAG	4 cut(s)	332, 861, 1578, 1661
BseNI	ACTGG	6 cut(s)	178, 569, 677, 1314, 1874, 2637
BseRI	GAGGAG	3 cut(s)	56, 887, 3267
BseSI	GKGCMC	2 cut(s)	1078, 1710
BseYI	CCCAGC	3 cut(s)	206, 314, 2382
BsgI	GTGCAG	2 cut(s)	1307, 1360
Bsh1236I	CGCG	1 cut(s)	75
BshFI	GGCC	7 cut(s)	903, 1314, 1414, 1950, 2348, 2487, 2661
BshNI	GGYRCC	1 cut(s)	1864
BsiHKAI	GWGCWC	4 cut(s)	997, 1078, 2191, 3061
BsiSI	CCGG	4 cut(s)	380, 575, 1477, 3279
BslFI	GGGAC	3 cut(s)	331, 1005, 1087
BslI	CCNNNNNNNGG	7 cut(s)	476, 581, 1880, 2053, 2356, 2695, 2998
BsmAI	GTCTC	4 cut(s)	148, 433, 2448, 3035
BsmBI	CGTCTC	2 cut(s)	148, 2448
BsmFI	GGGAC	3 cut(s)	331, 1005, 1087
BsmI	GAATGC	1 cut(s)	1825
BsnI	GGCC	7 cut(s)	903, 1314, 1414, 1950, 2348, 2487, 2661
Bsp119I	TTCGAA	1 cut(s)	3205
Bsp1286I	GDGCHC	6 cut(s)	207, 997, 1078, 1710, 2191, 3061
Bsp13I	TCCGGA	1 cut(s)	379
Bsp19I	CCATGG	3 cut(s)	1315, 1690, 3100
BspANI	GGCC	7 cut(s)	903, 1314, 1414, 1950, 2348, 2487, 2661
BspCNI	CTCAG	4 cut(s)	333, 862, 1579, 1660
BspEI	TCCGGA	1 cut(s)	379
BspFNI	CGCG	1 cut(s)	75
BspHI	TCATGA	1 cut(s)	538
BspLI	GGNNCC	4 cut(s)	384, 434, 1866, 3009
BspMI	ACCTGC	2 cut(s)	922, 2488
BspPI	GGATC	2 cut(s)	467, 1835
BspT104I	TTCGAA	1 cut(s)	3205
BspT107I	GGYRCC	1 cut(s)	1864
BsrBI	CCGCTC	1 cut(s)	35
BsrDI	GCAATG	2 cut(s)	296, 2121
BsrI	ACTGG	6 cut(s)	178, 569, 677, 1314, 1874, 2637
BssSI	CACGAG	1 cut(s)	1545
BssT1I	CCWWGG	6 cut(s)	1105, 1315, 1690, 1879, 2350, 3100
Bst2BI	CACGAG	1 cut(s)	1545
Bst2UI	CCWGG	4 cut(s)	254, 477, 1096, 2690
Bst4CI	ACNGT	7 cut(s)	363, 636, 858, 1777, 1783, 2366, 2782
Bst6I	CTCTTC	2 cut(s)	1224, 3045
BstAPI	GCANNNNNTGC	4 cut(s)	914, 2399, 2879, 2909
BstBI	TTCGAA	1 cut(s)	3205
BstDEI	CTNAG	6 cut(s)	341, 870, 1587, 1647, 1903, 2477
BstDSI	CCRYGG	4 cut(s)	362, 1315, 1690, 3100
BstFNI	CGCG	1 cut(s)	75
BstH2I	RGCGCY	1 cut(s)	250
BstHHI	GCGC	4 cut(s)	77, 249, 281, 1638
BstMAI	GTCTC	4 cut(s)	148, 433, 2448, 3035
BstNI	CCWGG	4 cut(s)	254, 477, 1096, 2690
BstNSI	RCATGY	3 cut(s)	833, 947, 2390
BstSCI	CCNGG	5 cut(s)	252, 475, 1094, 2688, 3277
BstSFI	CTRYAG	1 cut(s)	2418
BstSLI	GKGCMC	2 cut(s)	1078, 1710
BstUI	CGCG	1 cut(s)	75
BstV2I	GAAGAC	3 cut(s)	656, 1251, 1745
BstX2I	RGATCY	2 cut(s)	1276, 2410
BstXI	CCANNNNNNTGG	1 cut(s)	363
BstYI	RGATCY	2 cut(s)	1276, 2410
Bsu36I	CCTNAGG	1 cut(s)	341
BsuI	GTATCC	3 cut(s)	387, 401, 2218
BsuRI	GGCC	7 cut(s)	903, 1314, 1414, 1950, 2348, 2487, 2661
BtgI	CCRYGG	4 cut(s)	362, 1315, 1690, 3100
BtsIMutI	CAGTG	1 cut(s)	1076
BveI	ACCTGC	2 cut(s)	922, 2488
CaiI	CAGNNNCTG	2 cut(s)	683, 1270
CciI	TCATGA	1 cut(s)	538
CfoI	GCGC	4 cut(s)	77, 249, 281, 1638
Cfr13I	GGNCC	7 cut(s)	353, 1312, 1412, 1896, 2347, 3007, 3262
CseI	GACGC	1 cut(s)	2303
Csp6I	GTAC	4 cut(s)	1490, 2077, 2367, 3146
CviQI	GTAC	4 cut(s)	1490, 2077, 2367, 3146
DdeI	CTNAG	6 cut(s)	341, 870, 1587, 1647, 1903, 2477
DraI	TTTAAA	1 cut(s)	1891
DrdI	GACNNNNNNGTC	2 cut(s)	1099, 3122
DseDI	GACNNNNNNGTC	2 cut(s)	1099, 3122
EaeI	YGGCCR	1 cut(s)	2485
Eam1104I	CTCTTC	2 cut(s)	1224, 3045
EarI	CTCTTC	2 cut(s)	1224, 3045
EciI	GGCGGA	1 cut(s)	59
Ecl136II	GAGCTC	2 cut(s)	2189, 3059
Eco130I	CCWWGG	6 cut(s)	1105, 1315, 1690, 1879, 2350, 3100
Eco147I	AGGCCT	1 cut(s)	903
Eco24I	GRGCYC	3 cut(s)	207, 2191, 3061
Eco47I	GGWCC	4 cut(s)	353, 1896, 3007, 3262
Eco53kI	GAGCTC	2 cut(s)	2189, 3059
Eco57I	CTGAAG	6 cut(s)	201, 252, 710, 882, 1263, 3248
Eco81I	CCTNAGG	1 cut(s)	341
EcoICRI	GAGCTC	2 cut(s)	2189, 3059
EcoO109I	RGGNCCY	2 cut(s)	1412, 1896
EcoRI	GAATTC	1 cut(s)	3201
EcoRII	CCWGG	4 cut(s)	252, 475, 1094, 2688
EcoT14I	CCWWGG	6 cut(s)	1105, 1315, 1690, 1879, 2350, 3100
EcoT38I	GRGCYC	3 cut(s)	207, 2191, 3061
ErhI	CCWWGG	6 cut(s)	1105, 1315, 1690, 1879, 2350, 3100
Esp3I	CGTCTC	2 cut(s)	148, 2448
FalI	AAGNNNNNCTT	6 cut(s)	2256, 2288, 2896, 2928, 2928, 2960
FaqI	GGGAC	3 cut(s)	331, 1005, 1087
FauI	CCCGC	4 cut(s)	27, 62, 98, 1816
FauNDI	CATATG	1 cut(s)	775
FbaI	TGATCA	3 cut(s)	736, 922, 1438
FriOI	GRGCYC	3 cut(s)	207, 2191, 3061
GlaI	GCGC	4 cut(s)	76, 248, 280, 1637
GsaI	CCCAGC	3 cut(s)	210, 318, 2386
GsuI	CTGGAG	4 cut(s)	236, 419, 2321, 2562
HaeII	RGCGCY	1 cut(s)	250
HaeIII	GGCC	7 cut(s)	903, 1314, 1414, 1950, 2348, 2487, 2661
HapII	CCGG	4 cut(s)	380, 575, 1477, 3279
HgaI	GACGC	1 cut(s)	2303
HhaI	GCGC	4 cut(s)	77, 249, 281, 1638
Hin6I	GCGC	4 cut(s)	75, 247, 279, 1636
HinP1I	GCGC	4 cut(s)	75, 247, 279, 1636
HincII	GTYRAC	3 cut(s)	2258, 2332, 3115
HindII	GTYRAC	3 cut(s)	2258, 2332, 3115
HindIII	AAGCTT	4 cut(s)	213, 1329, 1990, 2730
HpaII	CCGG	4 cut(s)	380, 575, 1477, 3279
HphI	GGTGA	5 cut(s)	121, 167, 666, 685, 1331
Hpy166II	GTNNAC	6 cut(s)	639, 1076, 2258, 2332, 3007, 3115
Hpy8I	GTNNAC	6 cut(s)	639, 1076, 2258, 2332, 3007, 3115
Hpy99I	CGWCG	1 cut(s)	2670
HpyAV	CCTTC	8 cut(s)	93, 796, 1177, 1776, 1909, 1961, 2299, 3254
HpyCH4III	ACNGT	7 cut(s)	363, 636, 858, 1777, 1783, 2366, 2782
HpyCH4IV	ACGT	2 cut(s)	1248, 2441
HpyF3I	CTNAG	6 cut(s)	341, 870, 1587, 1647, 1903, 2477
HpySE526I	ACGT	2 cut(s)	1248, 2441
HspAI	GCGC	4 cut(s)	75, 247, 279, 1636
Kpn2I	TCCGGA	1 cut(s)	379
Ksp22I	TGATCA	3 cut(s)	736, 922, 1438
LmnI	GCTCC	5 cut(s)	311, 382, 438, 584, 781
MaeII	ACGT	2 cut(s)	1248, 2441
MaeIII	GTNAC	8 cut(s)	410, 672, 982, 998, 1290, 1757, 1777, 2428
MbiI	CCGCTC	1 cut(s)	35
MfeI	CAATTG	2 cut(s)	668, 2142
MflI	RGATCY	2 cut(s)	1276, 2410
MhlI	GDGCHC	6 cut(s)	207, 997, 1078, 1710, 2191, 3061
MlsI	TGGCCA	1 cut(s)	2487
MluNI	TGGCCA	1 cut(s)	2487
MlyI	GAGTC	4 cut(s)	580, 956, 1109, 1565
MmeI	TCCRAC	5 cut(s)	256, 289, 418, 989, 1655
Mox20I	TGGCCA	1 cut(s)	2487
MroI	TCCGGA	1 cut(s)	379
MroXI	GAANNNNTTC	4 cut(s)	2042, 2845, 2856, 3201
MscI	TGGCCA	1 cut(s)	2487
MseI	TTAA	7 cut(s)	744, 1088, 1730, 1890, 1983, 3084, 3168
MslI	CAYNNNNRTG	4 cut(s)	834, 1695, 2021, 2126
Msp20I	TGGCCA	1 cut(s)	2487
MspA1I	CMGCKG	1 cut(s)	1175
MspI	CCGG	4 cut(s)	380, 575, 1477, 3279
MspR9I	CCNGG	5 cut(s)	254, 477, 1096, 2690, 3279
MunI	CAATTG	2 cut(s)	668, 2142
Mva1269I	GAATGC	1 cut(s)	1825
MvaI	CCWGG	4 cut(s)	254, 477, 1096, 2690
MvnI	CGCG	1 cut(s)	75
NciI	CCSGG	1 cut(s)	3279
NcoI	CCATGG	3 cut(s)	1315, 1690, 3100
NdeI	CATATG	1 cut(s)	775
NlaIV	GGNNCC	4 cut(s)	384, 434, 1866, 3009
NmeAIII	GCCGAG	1 cut(s)	1489
NmuCI	GTSAC	3 cut(s)	672, 1777, 2428
NspI	RCATGY	3 cut(s)	833, 947, 2390
NspV	TTCGAA	1 cut(s)	3205
PaeI	GCATGC	2 cut(s)	833, 2390
PagI	TCATGA	1 cut(s)	538
PceI	AGGCCT	1 cut(s)	903
PctI	GAATGC	1 cut(s)	1825
PdmI	GAANNNNTTC	4 cut(s)	2042, 2845, 2856, 3201
PfeI	GAWTC	6 cut(s)	325, 711, 1369, 1796, 2269, 2670
PflFI	GACNNNGTC	1 cut(s)	1930
PflMI	CCANNNNNTGG	2 cut(s)	1880, 2695
PfoI	TCCNGGA	2 cut(s)	252, 475
PleI	GAGTC	4 cut(s)	579, 956, 1108, 1564
PpsI	GAGTC	4 cut(s)	579, 956, 1108, 1564
PpuMI	RGGWCCY	1 cut(s)	1896
Psp124BI	GAGCTC	2 cut(s)	2191, 3061
Psp5II	RGGWCCY	1 cut(s)	1896
Psp6I	CCWGG	4 cut(s)	252, 475, 1094, 2688
PspFI	CCCAGC	3 cut(s)	206, 314, 2382
PspGI	CCWGG	4 cut(s)	252, 475, 1094, 2688
PspN4I	GGNNCC	4 cut(s)	384, 434, 1866, 3009
PspPI	GGNCC	7 cut(s)	353, 1312, 1412, 1896, 2347, 3007, 3262
PspPPI	RGGWCCY	1 cut(s)	1896
PstNI	CAGNNNCTG	2 cut(s)	683, 1270
PsuI	RGATCY	2 cut(s)	1276, 2410
PsyI	GACNNNGTC	1 cut(s)	1930
RsaI	GTAC	4 cut(s)	1491, 2078, 2368, 3147
RsaNI	GTAC	4 cut(s)	1490, 2077, 2367, 3146
RseI	CAYNNNNRTG	4 cut(s)	834, 1695, 2021, 2126
SacI	GAGCTC	2 cut(s)	2191, 3061
SaqAI	TTAA	7 cut(s)	744, 1088, 1730, 1890, 1983, 3084, 3168
Sau96I	GGNCC	7 cut(s)	353, 1312, 1412, 1896, 2347, 3007, 3262
ScaI	AGTACT	1 cut(s)	2078
SchI	GAGTC	4 cut(s)	580, 956, 1109, 1565
ScrFI	CCNGG	5 cut(s)	254, 477, 1096, 2690, 3279
SduI	GDGCHC	6 cut(s)	207, 997, 1078, 1710, 2191, 3061
SfcI	CTRYAG	1 cut(s)	2418
SfuI	TTCGAA	1 cut(s)	3205
SinI	GGWCC	4 cut(s)	353, 1896, 3007, 3262
SmiMI	CAYNNNNRTG	4 cut(s)	834, 1695, 2021, 2126
SmlI	CTYRAG	4 cut(s)	38, 704, 1722, 2318
SmoI	CTYRAG	4 cut(s)	38, 704, 1722, 2318
SphI	GCATGC	2 cut(s)	833, 2390
SseBI	AGGCCT	1 cut(s)	903
SspI	AATATT	2 cut(s)	260, 1834
SstI	GAGCTC	2 cut(s)	2191, 3061
StuI	AGGCCT	1 cut(s)	903
StyD4I	CCNGG	5 cut(s)	252, 475, 1094, 2688, 3277
StyI	CCWWGG	6 cut(s)	1105, 1315, 1690, 1879, 2350, 3100
TaaI	ACNGT	7 cut(s)	363, 636, 858, 1777, 1783, 2366, 2782
TaiI	ACGT	2 cut(s)	1251, 2444
TatI	WGTACW	2 cut(s)	2076, 3145
TauI	GCSGC	2 cut(s)	75, 80
TfiI	GAWTC	6 cut(s)	325, 711, 1369, 1796, 2269, 2670
Tru1I	TTAA	7 cut(s)	744, 1088, 1730, 1890, 1983, 3084, 3168
Tru9I	TTAA	7 cut(s)	744, 1088, 1730, 1890, 1983, 3084, 3168
TscAI	CASTG	1 cut(s)	1076
TseFI	GTSAC	3 cut(s)	672, 1777, 2428
Tsp45I	GTSAC	3 cut(s)	672, 1777, 2428
TspDTI	ATGAA	9 cut(s)	338, 2005, 2110, 2187, 2211, 2777, 2791, 3060, 3210
TspGWI	ACGGA	1 cut(s)	2245
TspRI	CASTG	1 cut(s)	1076
Tth111I	GACNNNGTC	1 cut(s)	1930
Van91I	CCANNNNNTGG	2 cut(s)	1880, 2695
VneI	GTGCAC	1 cut(s)	1074
VpaK11BI	GGWCC	4 cut(s)	353, 1896, 3007, 3262
XapI	RAATTY	6 cut(s)	750, 2009, 2207, 2246, 2435, 3201
XbaI	TCTAGA	2 cut(s)	823, 965
XceI	RCATGY	3 cut(s)	833, 947, 2390
XmnI	GAANNNNTTC	4 cut(s)	2042, 2845, 2856, 3201
ZrmI	AGTACT	1 cut(s)	2078

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.