Rroxscaffold_4G00314550

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013556
Physical Location & Seq
Reverse (-)
38537161 .. 38542420
5260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00314550.1

Sequence Viewer

Length: 447 bp
ATGGTGAGCCGAAATTCCTCATGTTGTTCTTCTCCACTCAGAATCCAAATCCTCTTAGTATTGTCGTTGCTATTCTCTCCCGCAGTTCTCGCCAAGTCCCGTCGCCCAATCAGTGACATTGAGACAAGACAGAAGAAGACCCAATGTTATGCTGATATTGACAGTGGCTTGTGGGGATGGCAATGCAAGTCAACTATGATAGCAAAGGAGAATTGTGTTTTGAAATGCCTCTCTCCAACTTGTTATGAGCTCGTCTATGAGAGTGATCCGCTTGAAGAAGGAGAGAAAGATCTTCTTCTTAGAGGACAAGAGTTCAAGTACTGTATGCATAAGTATTCATGTTGGCTTGCAGGTTATCTTTGGGGGAGAGCCTTGAGGGTATTAAGGGCTCCTTTAATTATTAAAACTGAGTTCACAGCATCGCTTATAAGATATATGAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

148

Amino Acids

17.02

Weight (kDa)

8.98

Isoelectric Point (pI)

64.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4787 PF16029 39 - 108 4.9e-23 Domain of unknown function (DUF4787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013556)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17610
fragaria_vesca FvH4_7g07210 FvH4_7g07210 FvH4_7g07210
malus_domestica MD02G1238400.v1.1 MD07G1078200.v1.1
prunus_persica Prupe.2G100000_v2.0.a1 Prupe.2G100000_v2.0.a1
pyrus_communis pycom02g20380 pycom07g06190
rosa_chinensis RchiOBHm_Chr1g0337311
rosa_multiflora Rmu_sc0006824.1_g000023 Rmu_sc0022984.1_g000002
rosa_roxburghii Rroxscaffold_4G00314550
rosa_rugosa Rorug01G0134300.1
rosa_samantha Rh1AG153400 Rh1CG144300
rosa_wichuraiana Rw1G012560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 428
Acc36I ACCTGC 1 cut(s) 341
AciI CCGC 2 cut(s) 81, 269
AclWI GGATC 1 cut(s) 260
AcsI RAATTY 1 cut(s) 13
AfaI GTAC 1 cut(s) 320
AgsI TTSAA 3 cut(s) 223, 275, 316
AluBI AGCT 2 cut(s) 250, 442
AluI AGCT 2 cut(s) 250, 442
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 116
AlwI GGATC 1 cut(s) 260
ApoI RAATTY 1 cut(s) 13
AsuHPI GGTGA 1 cut(s) 16
BanII GRGCYC 2 cut(s) 252, 391
BbsI GAAGAC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 252
BccI CCATC 1 cut(s) 171
BcoDI GTCTC 1 cut(s) 116
BfuAI ACCTGC 1 cut(s) 341
BglII AGATCT 1 cut(s) 289
BmcAI AGTACT 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 390
BmsI GCATC 1 cut(s) 428
BpiI GAAGAC 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 188
BseGI GGATG 1 cut(s) 182
BseMI GCAATG 1 cut(s) 188
BseMII CTCAG 2 cut(s) 52, 399
BsiHKAI GWGCWC 1 cut(s) 252
BslFI GGGAC 1 cut(s) 82
BsmAI GTCTC 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 82
Bsp1286I GDGCHC 2 cut(s) 252, 391
Bsp143I GATC 2 cut(s) 265, 289
BspACI CCGC 2 cut(s) 81, 269
BspCNI CTCAG 2 cut(s) 51, 400
BspLI GGNNCC 1 cut(s) 390
BspMI ACCTGC 1 cut(s) 341
BspPI GGATC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 188
BssMI GATC 2 cut(s) 265, 289
Bst4CI ACNGT 2 cut(s) 164, 323
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 4 cut(s) 38, 55, 299, 408
BstF5I GGATG 1 cut(s) 182
BstKTI GATC 2 cut(s) 268, 292
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 2 cut(s) 265, 289
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 182
BtsIMutI CAGTG 2 cut(s) 118, 169
BveI ACCTGC 1 cut(s) 341
Cac8I GCNNGC 1 cut(s) 348
Csp6I GTAC 1 cut(s) 319
CviAII CATG 2 cut(s) 21, 339
CviJI RGCY 7 cut(s) 9, 168, 250, 346, 371, 389, 442
CviKI_1 RGCY 7 cut(s) 9, 168, 250, 346, 371, 389, 442
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 4 cut(s) 38, 55, 299, 408
DpnI GATC 2 cut(s) 267, 291
DpnII GATC 2 cut(s) 265, 289
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 2 cut(s) 252, 391
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoT22I ATGCAT 1 cut(s) 330
EcoT38I GRGCYC 2 cut(s) 252, 391
FaeI CATG 2 cut(s) 24, 342
FalI AAGNNNNNCTT 4 cut(s) 279, 311, 376, 408
FaqI GGGAC 1 cut(s) 82
FatI CATG 2 cut(s) 20, 338
FauI CCCGC 1 cut(s) 88
FokI GGATG 1 cut(s) 189
FriOI GRGCYC 2 cut(s) 252, 391
Hin1II CATG 2 cut(s) 24, 342
HincII GTYRAC 1 cut(s) 192
HindII GTYRAC 1 cut(s) 192
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 1 cut(s) 42
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 192, 414
Hpy188I TCNGA 1 cut(s) 41
Hpy8I GTNNAC 2 cut(s) 192, 414
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 1 cut(s) 272
HpyCH4III ACNGT 2 cut(s) 164, 323
HpyCH4V TGCA 3 cut(s) 186, 328, 350
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 4 cut(s) 38, 55, 299, 408
Hsp92II CATG 2 cut(s) 24, 342
Kzo9I GATC 2 cut(s) 265, 289
LmnI GCTCC 1 cut(s) 394
LpnPI CCDG 1 cut(s) 336
LweI GCATC 1 cut(s) 428
MaeIII GTNAC 1 cut(s) 113
MalI GATC 2 cut(s) 267, 291
MboI GATC 2 cut(s) 265, 289
MboII GAAGA 6 cut(s) 21, 145, 148, 284, 287, 287
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 2 cut(s) 252, 391
MluCI AATT 3 cut(s) 13, 211, 396
MmeI TCCRAC 1 cut(s) 260
MnlI CCTC 5 cut(s) 28, 62, 239, 296, 369
Mph1103I ATGCAT 1 cut(s) 330
MseI TTAA 3 cut(s) 383, 395, 402
MwoI GCNNNNNNNGC 1 cut(s) 89
NdeII GATC 2 cut(s) 265, 289
NlaIII CATG 2 cut(s) 24, 342
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 113
NsiI ATGCAT 1 cut(s) 330
PfeI GAWTC 1 cut(s) 42
PsiI TTATAA 1 cut(s) 428
Psp124BI GAGCTC 1 cut(s) 252
PspN4I GGNNCC 1 cut(s) 390
PsuI RGATCY 1 cut(s) 289
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 3 cut(s) 383, 395, 402
Sau3AI GATC 2 cut(s) 265, 289
ScaI AGTACT 1 cut(s) 320
SduI GDGCHC 2 cut(s) 252, 391
SetI ASST 3 cut(s) 252, 355, 444
SfaNI GCATC 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 3 cut(s) 13, 211, 396
SsiI CCGC 2 cut(s) 81, 269
SstI GAGCTC 1 cut(s) 252
TaaI ACNGT 2 cut(s) 164, 323
TasI AATT 3 cut(s) 13, 211, 396
TatI WGTACW 1 cut(s) 318
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 3 cut(s) 383, 395, 402
Tru9I TTAA 3 cut(s) 383, 395, 402
TscAI CASTG 2 cut(s) 118, 169
TseFI GTSAC 1 cut(s) 113
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 327
TspRI CASTG 2 cut(s) 118, 169
XapI RAATTY 1 cut(s) 13
ZrmI AGTACT 1 cut(s) 320
Zsp2I ATGCAT 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.