Rroxscaffold_4G00321640

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0021704
Physical Location & Seq
Reverse (-)
51265241 .. 51265845
605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321640.1

Sequence Viewer

Length: 396 bp
ATGGCTGAAGGACTAGTTAGCGTTCTCATCGACCGTTTGGTTACTATCACCAAGGACAAGATCCGAGGAGAGTTAAACTTGGTTACCGGAGTTGAGAAAGAAGTCAAAACTCTCACCGACAATCTCACGGCAATTCAATCTGTGCTCAAGGATGCGAGAGGGAGACAAGCGGTGGAGGCCGGCGTTCGGAGCTCGTGGTTCAACAATGGCGAAGGGGAGGTACGGCGGCCGGTTACGGAGCTCGAAATGGTCGACGTTCACACTCATCTTGTCGATGCTGTTCATGCCGACGGTGGTGCTTCGATGTTCATGGCCTTCGGAATCGTGTCGCTTCCGGAGAGCACCGACGACTCTTCGCCGAACGATCACGAGTTCCTTCCGCCGCAGGATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

131

Amino Acids

14.3

Weight (kDa)

4.78

Isoelectric Point (pI)

32.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 63 6.8e-09 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 252
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 4 cut(s) 170, 226, 380, 383
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 227
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 1 cut(s) 222
AfiI CCNNNNNNNGG 1 cut(s) 186
AgsI TTSAA 2 cut(s) 137, 202
AhlI ACTAGT 1 cut(s) 13
AluBI AGCT 2 cut(s) 192, 241
AluI AGCT 2 cut(s) 192, 241
Alw21I GWGCWC 4 cut(s) 147, 194, 243, 344
Alw26I GTCTC 1 cut(s) 157
AlwI GGATC 1 cut(s) 55
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 3 cut(s) 177, 227, 312
AsuHPI GGTGA 2 cut(s) 40, 106
BanII GRGCYC 2 cut(s) 194, 243
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
BauI CACGAG 2 cut(s) 193, 368
Bbv12I GWGCWC 4 cut(s) 147, 194, 243, 344
BceAI ACGGC 2 cut(s) 144, 239
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BcoDI GTCTC 1 cut(s) 157
BcuI ACTAGT 1 cut(s) 13
BfaI CTAG 1 cut(s) 14
BisI GCNGC 2 cut(s) 227, 383
BlsI GCNGC 2 cut(s) 228, 384
BmsI GCATC 2 cut(s) 142, 265
BpuEI CTTGAG 1 cut(s) 131
BsaJI CCNNGG 2 cut(s) 51, 64
BsaWI WCCGGW 2 cut(s) 86, 334
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse118I RCCGGY 2 cut(s) 179, 229
BseAI TCCGGA 1 cut(s) 334
BseDI CCNNGG 2 cut(s) 51, 64
BseGI GGATG 1 cut(s) 157
BseLI CCNNNNNNNGG 1 cut(s) 186
BseRI GAGGAG 1 cut(s) 81
BseX3I CGGCCG 1 cut(s) 227
Bsh1285I CGRYCG 2 cut(s) 34, 230
BshFI GGCC 3 cut(s) 179, 229, 314
BsiEI CGRYCG 2 cut(s) 34, 230
BsiHKAI GWGCWC 4 cut(s) 147, 194, 243, 344
BsiSI CCGG 4 cut(s) 87, 180, 230, 335
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 157
BsnI GGCC 3 cut(s) 179, 229, 314
Bsp1286I GDGCHC 4 cut(s) 147, 194, 243, 344
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 3 cut(s) 60, 364, 388
BspACI CCGC 4 cut(s) 170, 226, 380, 383
BspANI GGCC 3 cut(s) 179, 229, 314
BspEI TCCGGA 1 cut(s) 334
BspPI GGATC 1 cut(s) 55
BsrFI RCCGGY 2 cut(s) 179, 229
BssAI RCCGGY 2 cut(s) 179, 229
BssECI CCNNGG 2 cut(s) 51, 64
BssMI GATC 3 cut(s) 60, 364, 388
BssSI CACGAG 2 cut(s) 193, 368
BssT1I CCWWGG 1 cut(s) 51
Bst2BI CACGAG 2 cut(s) 193, 368
Bst4CI ACNGT 2 cut(s) 35, 293
Bst6I CTCTTC 1 cut(s) 358
BstC8I GCNNGC 1 cut(s) 181
BstEII GGTNACC 1 cut(s) 82
BstF5I GGATG 1 cut(s) 157
BstKTI GATC 3 cut(s) 63, 367, 391
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 3 cut(s) 60, 364, 388
BstMCI CGRYCG 2 cut(s) 34, 230
BstMWI GCNNNNNNNGC 3 cut(s) 176, 189, 284
BstPI GGTNACC 1 cut(s) 82
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BstZI CGGCCG 1 cut(s) 227
BsuRI GGCC 3 cut(s) 179, 229, 314
BtsCI GGATG 1 cut(s) 157
Cac8I GCNNGC 1 cut(s) 181
Cfr10I RCCGGY 2 cut(s) 179, 229
Csp6I GTAC 1 cut(s) 221
CviAII CATG 2 cut(s) 284, 310
CviJI RGCY 6 cut(s) 5, 179, 192, 229, 241, 314
CviKI_1 RGCY 6 cut(s) 5, 179, 192, 229, 241, 314
CviQI GTAC 1 cut(s) 221
DpnI GATC 3 cut(s) 62, 366, 390
DpnII GATC 3 cut(s) 60, 364, 388
EaeI YGGCCR 1 cut(s) 227
EagI CGGCCG 1 cut(s) 227
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
EciI GGCGGA 1 cut(s) 369
Ecl136II GAGCTC 2 cut(s) 192, 241
EclXI CGGCCG 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 2 cut(s) 194, 243
Eco52I CGGCCG 1 cut(s) 227
Eco53kI GAGCTC 2 cut(s) 192, 241
Eco57I CTGAAG 1 cut(s) 27
Eco91I GGTNACC 1 cut(s) 82
EcoICRI GAGCTC 2 cut(s) 192, 241
EcoO65I GGTNACC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 194, 243
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 2 cut(s) 287, 313
FaiI YATR 2 cut(s) 285, 311
FatI CATG 2 cut(s) 283, 309
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 2 cut(s) 227, 383
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 2 cut(s) 194, 243
Fsp4HI GCNGC 2 cut(s) 227, 383
FspBI CTAG 1 cut(s) 14
GluI GCNGC 2 cut(s) 227, 383
HaeIII GGCC 3 cut(s) 179, 229, 314
HapII CCGG 4 cut(s) 87, 180, 230, 335
Hin1II CATG 2 cut(s) 287, 313
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 2 cut(s) 321, 350
HpaII CCGG 4 cut(s) 87, 180, 230, 335
HphI GGTGA 2 cut(s) 40, 106
Hpy166II GTNNAC 2 cut(s) 253, 259
Hpy188I TCNGA 3 cut(s) 65, 189, 320
Hpy188III TCNNGA 2 cut(s) 335, 368
Hpy8I GTNNAC 2 cut(s) 253, 259
Hpy99I CGWCG 3 cut(s) 257, 293, 350
HpyAV CCTTC 3 cut(s) 206, 325, 386
HpyCH4III ACNGT 2 cut(s) 35, 293
HpyCH4IV ACGT 1 cut(s) 255
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 189, 284
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 2 cut(s) 287, 313
Kpn2I TCCGGA 1 cut(s) 334
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Kzo9I GATC 3 cut(s) 60, 364, 388
LmnI GCTCC 2 cut(s) 189, 238
LpnPI CCDG 5 cut(s) 100, 193, 243, 348, 371
LweI GCATC 2 cut(s) 142, 265
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 40, 82, 232
MalI GATC 3 cut(s) 62, 366, 390
MboI GATC 3 cut(s) 60, 364, 388
MboII GAAGA 1 cut(s) 345
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 4 cut(s) 147, 194, 243, 344
MluCI AATT 1 cut(s) 132
MlyI GAGTC 1 cut(s) 344
MnlI CCTC 4 cut(s) 59, 152, 169, 211
MroI TCCGGA 1 cut(s) 334
MroNI GCCGGC 1 cut(s) 179
MseI TTAA 1 cut(s) 74
MspI CCGG 4 cut(s) 87, 180, 230, 335
MwoI GCNNNNNNNGC 3 cut(s) 176, 189, 284
NaeI GCCGGC 1 cut(s) 181
NdeII GATC 3 cut(s) 60, 364, 388
NgoMIV GCCGGC 1 cut(s) 179
NlaIII CATG 2 cut(s) 287, 313
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PdiI GCCGGC 1 cut(s) 181
PfeI GAWTC 1 cut(s) 321
PkrI GCNGC 2 cut(s) 228, 384
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
Psp124BI GAGCTC 2 cut(s) 194, 243
PspEI GGTNACC 1 cut(s) 82
PsuI RGATCY 1 cut(s) 60
RsaI GTAC 1 cut(s) 222
RsaNI GTAC 1 cut(s) 221
SacI GAGCTC 2 cut(s) 194, 243
SalI GTCGAC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 2 cut(s) 227, 383
Sau3AI GATC 3 cut(s) 60, 364, 388
SchI GAGTC 1 cut(s) 344
SduI GDGCHC 4 cut(s) 147, 194, 243, 344
SetI ASST 4 cut(s) 194, 222, 243, 258
SfaNI GCATC 2 cut(s) 142, 265
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SpeI ACTAGT 1 cut(s) 13
Sse9I AATT 1 cut(s) 132
SsiI CCGC 4 cut(s) 170, 226, 380, 383
SspMI CTAG 1 cut(s) 14
SstI GAGCTC 2 cut(s) 194, 243
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 2 cut(s) 35, 293
TaiI ACGT 1 cut(s) 258
TaqI TCGA 5 cut(s) 30, 243, 252, 273, 302
TasI AATT 1 cut(s) 132
TauI GCSGC 2 cut(s) 229, 385
TfiI GAWTC 1 cut(s) 321
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TspDTI ATGAA 2 cut(s) 272, 298
TspGWI ACGGA 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 252
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.