Rroxscaffold_4G00327280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0019497
Physical Location & Seq
Reverse (-)
59889158 .. 59898257
9100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327280.1

Sequence Viewer

Length: 972 bp
ATGGGACCAACAAGGAAGGTACAGAACAAAATGGAATTACTCAAGAAGAAGGAAAAGCCCAAGTACGTACTATTGATCAGTGAGCAGTCAAAGAATGAATTTGGGACTAAAGAAGAGCAAGTATTGATCAGCAAGTTAAAGAATGAATTGTCTGATGATCGACTTGAACACTGCAAACATATTGACGGGTGCTCTTCAAGATGCCTTGAGATTTATAAACACTTCTTTGAAACCATTCAAGATGCATCCCGAGCAACAGGAATTATATCTGTCACAAACAGAAATGGTGTCGGCCCAGTCGGTGGGGGCCCAGTCCATGTCAGCTCGGTCAGTGAGGACAAAAGTTGCAGCAGAACAGTGGGGTCCAGCCGACAACCTTACCAGTTGACTAGCATTGATGTGACCAGCCAATTGTCAAAAGATACTAACGCAGCACCCCAAGATTTTAATATGCATTCCCAAAGTGACCTCCACCTGTTGAACCGAGAATGTGCTACAAGCCAAGAAATTGGCACTCCTCTTGAGGTTGACAACGACGCCTCAACGCATCCTAAAATATATGAAATATCACAAACTGCCGAAACAAGTGAATTCAAGGAACCCGACCGGTTACTTGACAACTACATTTCCGTTTCTTTACCTCTCGTGGTGTTTGATTTCAAAACAAAAGGGACCAATGTGTTAATCATAAAACTAGGAGGAGGTAGAATGCATTCGAGGAAAAAAGGGAAGTGGCTGATTGATCCAGGAGAGTTGACTACCCGGAGTAGGTCTCAAGTCGGAGACAAAGGCATAGCAAGGGAGGTGAAGGAAATTTGCTACCTTCGGTTCCGGTGCGATGCCCAGCCTCCGGCTTCACCAATCACATTCTCATCAGCGAAACCCCTTGTCATGGGAAATGCATCAAATATAGTTTGTGGTGGAAAGGGAACAAAAGGAAATCTGTTTTTGATTGTAAGATTGTCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

323

Amino Acids

35.68

Weight (kDa)

8.9

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019497)

Species Orthologous Gene IDs
rosa_laevigata RLG00000031965
rosa_multiflora Rmu_sc0000252.1_g000015
rosa_roxburghii Rroxscaffold_1G00063700 Rroxscaffold_1G00063710 Rroxscaffold_4G00327280
rosa_rugosa Rorug04G0399700 Rorug05G0005000
rosa_samantha Rh5CG025000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 216
AccB7I CCANNNNNTGG 1 cut(s) 302
AclWI GGATC 1 cut(s) 737
AcsI RAATTY 3 cut(s) 98, 590, 813
AcyI GRCGYC 1 cut(s) 537
AfaI GTAC 3 cut(s) 21, 65, 69
AfiI CCNNNNNNNGG 4 cut(s) 302, 768, 850, 892
AgeI ACCGGT 1 cut(s) 606
AgsI TTSAA 7 cut(s) 167, 198, 230, 239, 481, 595, 661
AjnI CCWGG 1 cut(s) 745
AluBI AGCT 1 cut(s) 324
AluI AGCT 1 cut(s) 324
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 2 cut(s) 777, 777
AlwI GGATC 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 249
AoxI GGCC 2 cut(s) 292, 307
ApaI GGGCCC 1 cut(s) 311
ApeKI GCWGC 2 cut(s) 348, 431
ApoI RAATTY 3 cut(s) 98, 590, 813
AsiGI ACCGGT 1 cut(s) 606
Asp700I GAANNNNTTC 2 cut(s) 234, 712
AspS9I GGNCC 6 cut(s) 5, 293, 307, 308, 363, 672
AsuC2I CCSGG 1 cut(s) 763
AsuHPI GGTGA 2 cut(s) 817, 849
AvaI CYCGRG 1 cut(s) 249
AvaII GGWCC 3 cut(s) 5, 363, 672
BaeGI GKGCMC 1 cut(s) 311
BanII GRGCYC 1 cut(s) 311
BauI CACGAG 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 360, 443
BciT130I CCWGG 1 cut(s) 747
BclI TGATCA 2 cut(s) 75, 126
BcnI CCSGG 1 cut(s) 763
BcoDI GTCTC 2 cut(s) 777, 777
BfaI CTAG 2 cut(s) 390, 695
BisI GCNGC 2 cut(s) 349, 432
BlsI GCNGC 2 cut(s) 350, 433
Bme1390I CCNGG 2 cut(s) 747, 763
Bme18I GGWCC 3 cut(s) 5, 363, 672
BmeT110I CYCGRG 1 cut(s) 249
BmgT120I GGNCC 6 cut(s) 5, 293, 307, 308, 363, 672
BmiI GGNNCC 7 cut(s) 6, 308, 309, 364, 600, 673, 830
BmrFI CCNGG 2 cut(s) 747, 763
BmrI ACTGGG 2 cut(s) 290, 305
BmsI GCATC 6 cut(s) 191, 232, 254, 556, 829, 911
BmuI ACTGGG 2 cut(s) 290, 305
BplI GAGNNNNNCTC 2 cut(s) 757, 789
BpuEI CTTGAG 4 cut(s) 26, 227, 542, 759
BpuMI CCSGG 1 cut(s) 763
BsaAI YACGTR 1 cut(s) 67
BsaHI GRCGYC 1 cut(s) 537
BsaI GGTCTC 1 cut(s) 777
BsaWI WCCGGW 2 cut(s) 606, 831
Bsc4I CCNNNNNNNGG 4 cut(s) 302, 768, 850, 892
Bse118I RCCGGY 1 cut(s) 606
Bse1I ACTGG 3 cut(s) 296, 311, 382
BseBI CCWGG 1 cut(s) 747
BseGI GGATG 2 cut(s) 245, 547
BseLI CCNNNNNNNGG 4 cut(s) 302, 768, 850, 892
BseNI ACTGG 3 cut(s) 296, 311, 382
BseRI GAGGAG 2 cut(s) 507, 714
BseSI GKGCMC 1 cut(s) 311
BseXI GCAGC 2 cut(s) 360, 443
BseYI CCCAGC 1 cut(s) 843
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 2 cut(s) 294, 309
BshTI ACCGGT 1 cut(s) 606
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 194
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 4 cut(s) 607, 763, 832, 851
BslFI GGGAC 3 cut(s) 18, 118, 685
BslI CCNNNNNNNGG 4 cut(s) 302, 768, 850, 892
BsmAI GTCTC 2 cut(s) 777, 777
BsmFI GGGAC 3 cut(s) 18, 118, 685
BsmI GAATGC 3 cut(s) 454, 712, 714
BsnI GGCC 2 cut(s) 294, 309
Bso31I GGTCTC 1 cut(s) 777
BsoBI CYCGRG 1 cut(s) 249
Bsp120I GGGCCC 1 cut(s) 307
Bsp1286I GDGCHC 2 cut(s) 194, 311
Bsp143I GATC 4 cut(s) 75, 126, 157, 742
BspANI GGCC 2 cut(s) 294, 309
BspLI GGNNCC 7 cut(s) 6, 308, 309, 364, 600, 673, 830
BspPI GGATC 1 cut(s) 737
BspQI GCTCTTC 2 cut(s) 108, 199
BspTNI GGTCTC 1 cut(s) 777
BsrFI RCCGGY 1 cut(s) 606
BsrI ACTGG 3 cut(s) 296, 311, 382
BssAI RCCGGY 1 cut(s) 606
BssMI GATC 4 cut(s) 75, 126, 157, 742
BssNI GRCGYC 1 cut(s) 537
BssSI CACGAG 1 cut(s) 644
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 1 cut(s) 358
Bst6I CTCTTC 2 cut(s) 108, 199
BstACI GRCGYC 1 cut(s) 537
BstBAI YACGTR 1 cut(s) 67
BstF5I GGATG 2 cut(s) 245, 547
BstKTI GATC 4 cut(s) 78, 129, 160, 745
BstMAI GTCTC 2 cut(s) 777, 777
BstMBI GATC 4 cut(s) 75, 126, 157, 742
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 1 cut(s) 747
BstSCI CCNGG 2 cut(s) 745, 761
BstSLI GKGCMC 1 cut(s) 311
BstSNI TACGTA 1 cut(s) 67
BstV1I GCAGC 2 cut(s) 360, 443
BstXI CCANNNNNNTGG 1 cut(s) 509
BsuRI GGCC 2 cut(s) 294, 309
BtgZI GCGATG 1 cut(s) 852
BtsCI GGATG 2 cut(s) 245, 547
BtsI GCAGTG 1 cut(s) 169
BtsIMutI CAGTG 4 cut(s) 85, 169, 337, 363
Cfr10I RCCGGY 1 cut(s) 606
Cfr13I GGNCC 6 cut(s) 5, 293, 307, 308, 363, 672
CseI GACGC 1 cut(s) 545
Csp6I GTAC 3 cut(s) 20, 64, 68
CspAI ACCGGT 1 cut(s) 606
CviAII CATG 2 cut(s) 317, 892
CviQI GTAC 3 cut(s) 20, 64, 68
DpnI GATC 4 cut(s) 77, 128, 159, 744
DpnII GATC 4 cut(s) 75, 126, 157, 742
Eam1104I CTCTTC 2 cut(s) 108, 199
EarI CTCTTC 2 cut(s) 108, 199
Eco105I TACGTA 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 311
Eco31I GGTCTC 1 cut(s) 777
Eco47I GGWCC 3 cut(s) 5, 363, 672
Eco88I CYCGRG 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 307
EcoRI GAATTC 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 745
EcoT22I ATGCAT 4 cut(s) 247, 456, 714, 904
EcoT38I GRGCYC 1 cut(s) 311
FaeI CATG 2 cut(s) 320, 895
FaqI GGGAC 3 cut(s) 18, 118, 685
FatI CATG 2 cut(s) 316, 891
FbaI TGATCA 2 cut(s) 75, 126
Fnu4HI GCNGC 2 cut(s) 349, 432
FokI GGATG 2 cut(s) 232, 534
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 349, 432
FspBI CTAG 2 cut(s) 390, 695
GluI GCNGC 2 cut(s) 349, 432
GsaI CCCAGC 1 cut(s) 847
HaeIII GGCC 2 cut(s) 294, 309
HapII CCGG 4 cut(s) 607, 763, 832, 851
HgaI GACGC 1 cut(s) 545
Hin1I GRCGYC 1 cut(s) 537
Hin1II CATG 2 cut(s) 320, 895
HincII GTYRAC 4 cut(s) 387, 529, 756, 966
HindII GTYRAC 4 cut(s) 387, 529, 756, 966
HpaII CCGG 4 cut(s) 607, 763, 832, 851
HphI GGTGA 2 cut(s) 817, 849
Hpy166II GTNNAC 4 cut(s) 387, 529, 756, 966
Hpy188I TCNGA 2 cut(s) 154, 782
Hpy188III TCNNGA 5 cut(s) 43, 198, 239, 249, 521
Hpy8I GTNNAC 4 cut(s) 387, 529, 756, 966
Hpy99I CGWCG 1 cut(s) 539
HpyAV CCTTC 4 cut(s) 10, 43, 802, 833
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 6 cut(s) 174, 245, 348, 454, 712, 902
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 537
Hsp92II CATG 2 cut(s) 320, 895
Ksp22I TGATCA 2 cut(s) 75, 126
Kzo9I GATC 4 cut(s) 75, 126, 157, 742
LguI GCTCTTC 2 cut(s) 108, 199
Lsp1109I GCAGC 2 cut(s) 360, 443
LweI GCATC 6 cut(s) 191, 232, 254, 556, 829, 911
MaeI CTAG 2 cut(s) 390, 695
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 4 cut(s) 271, 400, 464, 609
MalI GATC 4 cut(s) 77, 128, 159, 744
MboI GATC 4 cut(s) 75, 126, 157, 742
MboII GAAGA 3 cut(s) 58, 125, 186
MfeI CAATTG 1 cut(s) 410
MhlI GDGCHC 2 cut(s) 194, 311
MluCI AATT 8 cut(s) 35, 98, 146, 261, 410, 507, 590, 813
MmeI TCCRAC 1 cut(s) 760
Mph1103I ATGCAT 4 cut(s) 247, 456, 714, 904
MroXI GAANNNNTTC 2 cut(s) 234, 712
MseI TTAA 3 cut(s) 137, 447, 683
MslI CAYNNNNRTG 1 cut(s) 398
MspI CCGG 4 cut(s) 607, 763, 832, 851
MspR9I CCNGG 2 cut(s) 747, 763
MunI CAATTG 1 cut(s) 410
Mva1269I GAATGC 3 cut(s) 454, 712, 714
MvaI CCWGG 1 cut(s) 747
MwoI GCNNNNNNNGC 1 cut(s) 251
NciI CCSGG 1 cut(s) 763
NdeII GATC 4 cut(s) 75, 126, 157, 742
NlaIII CATG 2 cut(s) 320, 895
NlaIV GGNNCC 7 cut(s) 6, 308, 309, 364, 600, 673, 830
NmuCI GTSAC 3 cut(s) 271, 400, 464
NsiI ATGCAT 4 cut(s) 247, 456, 714, 904
PciSI GCTCTTC 2 cut(s) 108, 199
PctI GAATGC 3 cut(s) 454, 712, 714
PdmI GAANNNNTTC 2 cut(s) 234, 712
PflMI CCANNNNNTGG 1 cut(s) 302
PfoI TCCNGGA 1 cut(s) 745
PinAI ACCGGT 1 cut(s) 606
PkrI GCNGC 2 cut(s) 350, 433
Ppu21I YACGTR 1 cut(s) 67
PsiI TTATAA 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 745
PspFI CCCAGC 1 cut(s) 843
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 7 cut(s) 6, 308, 309, 364, 600, 673, 830
PspOMI GGGCCC 1 cut(s) 307
PspPI GGNCC 6 cut(s) 5, 293, 307, 308, 363, 672
RsaI GTAC 3 cut(s) 21, 65, 69
RsaNI GTAC 3 cut(s) 20, 64, 68
RseI CAYNNNNRTG 1 cut(s) 398
SapI GCTCTTC 2 cut(s) 108, 199
SaqAI TTAA 3 cut(s) 137, 447, 683
SatI GCNGC 2 cut(s) 349, 432
Sau3AI GATC 4 cut(s) 75, 126, 157, 742
Sau96I GGNCC 6 cut(s) 5, 293, 307, 308, 363, 672
ScrFI CCNGG 2 cut(s) 747, 763
SduI GDGCHC 2 cut(s) 194, 311
SfaNI GCATC 6 cut(s) 191, 232, 254, 556, 829, 911
SinI GGWCC 3 cut(s) 5, 363, 672
SmiMI CAYNNNNRTG 1 cut(s) 398
SmlI CTYRAG 4 cut(s) 41, 206, 521, 774
SmoI CTYRAG 4 cut(s) 41, 206, 521, 774
SnaBI TACGTA 1 cut(s) 67
Sse9I AATT 8 cut(s) 35, 98, 146, 261, 410, 507, 590, 813
SspMI CTAG 2 cut(s) 390, 695
StyD4I CCNGG 2 cut(s) 745, 761
TaaI ACNGT 1 cut(s) 358
TaiI ACGT 1 cut(s) 69
TaqI TCGA 2 cut(s) 160, 716
TaqII GACCGA 1 cut(s) 316
TasI AATT 8 cut(s) 35, 98, 146, 261, 410, 507, 590, 813
Tru1I TTAA 3 cut(s) 137, 447, 683
Tru9I TTAA 3 cut(s) 137, 447, 683
TscAI CASTG 4 cut(s) 85, 176, 337, 363
TseFI GTSAC 3 cut(s) 271, 400, 464
TseI GCWGC 2 cut(s) 348, 431
Tsp45I GTSAC 3 cut(s) 271, 400, 464
TspDTI ATGAA 3 cut(s) 111, 159, 576
TspGWI ACGGA 1 cut(s) 619
TspRI CASTG 4 cut(s) 85, 176, 337, 363
Van91I CCANNNNNTGG 1 cut(s) 302
VpaK11BI GGWCC 3 cut(s) 5, 363, 672
XapI RAATTY 3 cut(s) 98, 590, 813
XmnI GAANNNNTTC 2 cut(s) 234, 712
XspI CTAG 2 cut(s) 390, 695
Zsp2I ATGCAT 4 cut(s) 247, 456, 714, 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.