Rroxscaffold_5G00367560

SPFH domain / Band 7 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0018894
Physical Location & Seq
Reverse (-)
49369279 .. 49373735
4457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367560.1

Sequence Viewer

Length: 762 bp
ATGTCAAGGAGCTCGTCCAGGCCAGGGCATCATCGAGGGCGAGACACGTGTGCTCACAGCCTCGATGACGATGGAGGAGGTCTTCAAAGGGACCAAGGAGTTCAAGAAGGAGGTGTTTGGGAAGGTTCAGCTGGAGTTGGATCAGTTCGGGCTTTTGATATACAATGCCAATGGGCAGACATTGCAAAACGGAGGCAAGATCGACTCCTAGACGAAGATCATTTTGACTCAGAGGCAAAGGGACTAGGGGAGGGGAAGAAGGAGGAGATTAAGGTGAAAACGGAGGTCAAGGCTAGGTTTATGAGAATCAGATCGAGAGAAGCTGAGGTGGCTAAAGCGAATGCTGAGTTGGCAATGAAGAAGGCAGGGTGGGCTAAGAAGGCGCAGGTGGCCGAGGTTAAGGCGGAGAAGGCGGTGGGGATGAAGGATGCCGAGTTGCAGAAGGAGGTGGAGAGGATGAATGCATTGACTATGACTGAGAAGCTCAAGGCTGACTTCTTGAGCAAGGGTAATGTCGAATATGAAACCAAGCTGATCGATGATGGAGAATTCTATGCCAAGAAGAAAGAGGCTGAGGGACTAATTGCACTTGGCCAAGTCCAGGCTACGCCACTGGCCATGAGGGACTTCTTGATGATCAATGGTGGGATGTTTCAAGAAATTGCTAAGATTAATGCCAAGGCTGTGAGAGGGCTTGTGCCGAAGGTTAGTATCTGGACTAATGGAGTGGAGGGCGTGGATGGTGGTGGCAACAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Band_7

Protein Analysis

253

Amino Acids

27.74

Weight (kDa)

6.94

Isoelectric Point (pI)

27.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018894)

Species Orthologous Gene IDs
malus_domestica MD16G1287500.v1.1
rosa_chinensis RchiOBHm_Chr1g0356261
rosa_multiflora Rmu_co8276241.1_g000001
rosa_roxburghii Rroxscaffold_5G00367560 Rroxscaffold_7G00170660
rosa_rugosa Rorug04G0204300
rosa_samantha Rh4AG261600 Rh4BG267500 Rh4CG278600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 376
Acc36I ACCTGC 1 cut(s) 376
AciI CCGC 2 cut(s) 404, 413
AclWI GGATC 1 cut(s) 148
AcoI YGGCCR 3 cut(s) 390, 592, 615
AcsI RAATTY 1 cut(s) 548
AcvI CACGTG 1 cut(s) 48
AfiI CCNNNNNNNGG 2 cut(s) 24, 601
AflIII ACRYGT 2 cut(s) 45, 47
AgsI TTSAA 3 cut(s) 86, 104, 656
AjnI CCWGG 3 cut(s) 17, 22, 600
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 5 cut(s) 12, 131, 323, 484, 532
AluI AGCT 5 cut(s) 12, 131, 323, 484, 532
Alw21I GWGCWC 2 cut(s) 14, 55
Alw26I GTCTC 1 cut(s) 36
AlwI GGATC 1 cut(s) 148
AoxI GGCC 4 cut(s) 20, 390, 592, 615
ApoI RAATTY 1 cut(s) 548
AseI ATTAAT 1 cut(s) 672
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 1 cut(s) 286
AvaII GGWCC 1 cut(s) 91
BalI TGGCCA 2 cut(s) 594, 617
BanII GRGCYC 1 cut(s) 14
BbrPI CACGTG 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 74
Bbv12I GWGCWC 2 cut(s) 14, 55
BbvCI CCTCAGC 2 cut(s) 324, 573
BccI CCATC 3 cut(s) 65, 536, 734
BciT130I CCWGG 3 cut(s) 19, 24, 602
BclI TGATCA 1 cut(s) 636
BcoDI GTCTC 1 cut(s) 36
BfaI CTAG 3 cut(s) 209, 245, 294
BfuAI ACCTGC 1 cut(s) 376
Bme1390I CCNGG 3 cut(s) 19, 24, 602
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 3 cut(s) 19, 24, 602
BmsI GCATC 2 cut(s) 37, 418
BpiI GAAGAC 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 153
Bpu10I CCTNAGC 2 cut(s) 324, 573
BpuEI CTTGAG 2 cut(s) 470, 520
Bsa29I ATCGAT 1 cut(s) 537
BsaAI YACGTR 1 cut(s) 48
BsaJI CCNNGG 4 cut(s) 23, 94, 393, 678
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 601
Bse1I ACTGG 1 cut(s) 618
Bse3DI GCAATG 2 cut(s) 180, 360
BseBI CCWGG 3 cut(s) 19, 24, 602
BseCI ATCGAT 1 cut(s) 537
BseDI CCNNGG 4 cut(s) 23, 94, 393, 678
BseGI GGATG 5 cut(s) 426, 433, 462, 654, 745
BseLI CCNNNNNNNGG 2 cut(s) 24, 601
BseMI GCAATG 2 cut(s) 180, 360
BseMII CTCAG 5 cut(s) 243, 315, 336, 468, 564
BseNI ACTGG 1 cut(s) 618
BseRI GAGGAG 2 cut(s) 90, 278
BshFI GGCC 4 cut(s) 22, 392, 594, 617
BshVI ATCGAT 1 cut(s) 537
BsiHKAI GWGCWC 2 cut(s) 14, 55
BslFI GGGAC 4 cut(s) 104, 255, 591, 638
BslI CCNNNNNNNGG 2 cut(s) 24, 601
BsmAI GTCTC 1 cut(s) 36
BsmFI GGGAC 4 cut(s) 104, 255, 591, 638
BsmI GAATGC 2 cut(s) 346, 466
BsnI GGCC 4 cut(s) 22, 392, 594, 617
Bsp1286I GDGCHC 2 cut(s) 14, 55
Bsp143I GATC 6 cut(s) 140, 199, 217, 311, 534, 636
BspACI CCGC 2 cut(s) 404, 413
BspANI GGCC 4 cut(s) 22, 392, 594, 617
BspCNI CTCAG 5 cut(s) 242, 316, 337, 469, 565
BspDI ATCGAT 1 cut(s) 537
BspLI GGNNCC 1 cut(s) 92
BspMI ACCTGC 1 cut(s) 376
BspPI GGATC 1 cut(s) 148
BsrDI GCAATG 2 cut(s) 180, 360
BsrI ACTGG 1 cut(s) 618
BssECI CCNNGG 4 cut(s) 23, 94, 393, 678
BssMI GATC 6 cut(s) 140, 199, 217, 311, 534, 636
BssT1I CCWWGG 2 cut(s) 94, 678
Bst2UI CCWGG 3 cut(s) 19, 24, 602
BstAPI GCANNNNNTGC 1 cut(s) 182
BstBAI YACGTR 1 cut(s) 48
BstDEI CTNAG 7 cut(s) 229, 324, 345, 375, 477, 573, 666
BstF5I GGATG 5 cut(s) 426, 433, 462, 654, 745
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 6 cut(s) 143, 202, 220, 314, 537, 639
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 6 cut(s) 140, 199, 217, 311, 534, 636
BstMWI GCNNNNNNNGC 7 cut(s) 182, 329, 350, 371, 380, 389, 410
BstNI CCWGG 3 cut(s) 19, 24, 602
BstSCI CCNGG 3 cut(s) 17, 22, 600
BstV2I GAAGAC 1 cut(s) 74
Bsu15I ATCGAT 1 cut(s) 537
BsuRI GGCC 4 cut(s) 22, 392, 594, 617
BsuTUI ATCGAT 1 cut(s) 537
BtsCI GGATG 5 cut(s) 426, 433, 462, 654, 745
BtsIMutI CAGTG 1 cut(s) 611
BveI ACCTGC 1 cut(s) 376
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 1 cut(s) 91
ClaI ATCGAT 1 cut(s) 537
CviAII CATG 1 cut(s) 619
DdeI CTNAG 7 cut(s) 229, 324, 345, 375, 477, 573, 666
DpnI GATC 6 cut(s) 142, 201, 219, 313, 536, 638
DpnII GATC 6 cut(s) 140, 199, 217, 311, 534, 636
EaeI YGGCCR 3 cut(s) 390, 592, 615
EciI GGCGGA 1 cut(s) 419
Ecl136II GAGCTC 1 cut(s) 12
Eco130I CCWWGG 2 cut(s) 94, 678
Eco24I GRGCYC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 12
Eco72I CACGTG 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 3 cut(s) 17, 22, 600
EcoT14I CCWWGG 2 cut(s) 94, 678
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 14
ErhI CCWWGG 2 cut(s) 94, 678
FaeI CATG 1 cut(s) 622
FaiI YATR 6 cut(s) 161, 302, 473, 522, 555, 620
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 4 cut(s) 104, 255, 591, 638
FatI CATG 1 cut(s) 618
FbaI TGATCA 1 cut(s) 636
FokI GGATG 5 cut(s) 433, 440, 469, 661, 752
FriOI GRGCYC 1 cut(s) 14
FspBI CTAG 3 cut(s) 209, 245, 294
GlaI GCGC 1 cut(s) 384
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 4 cut(s) 22, 392, 594, 617
HhaI GCGC 1 cut(s) 385
Hin1II CATG 1 cut(s) 622
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HinfI GANTC 3 cut(s) 204, 227, 306
HphI GGTGA 1 cut(s) 286
Hpy188I TCNGA 2 cut(s) 232, 311
Hpy188III TCNNGA 6 cut(s) 104, 315, 499, 631, 656, 715
HpyAV CCTTC 9 cut(s) 101, 116, 253, 355, 373, 403, 418, 436, 697
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 4 cut(s) 185, 439, 464, 587
HpyF10VI GCNNNNNNNGC 7 cut(s) 182, 329, 350, 371, 380, 389, 410
HpyF3I CTNAG 7 cut(s) 229, 324, 345, 375, 477, 573, 666
HpySE526I ACGT 1 cut(s) 47
Hsp92II CATG 1 cut(s) 622
HspAI GCGC 1 cut(s) 383
Ksp22I TGATCA 1 cut(s) 636
Kzo9I GATC 6 cut(s) 140, 199, 217, 311, 534, 636
LmnI GCTCC 1 cut(s) 9
LweI GCATC 2 cut(s) 37, 418
MaeI CTAG 3 cut(s) 209, 245, 294
MaeII ACGT 1 cut(s) 47
MalI GATC 6 cut(s) 142, 201, 219, 313, 536, 638
MboI GATC 6 cut(s) 140, 199, 217, 311, 534, 636
MboII GAAGA 5 cut(s) 74, 227, 268, 370, 574
MhlI GDGCHC 2 cut(s) 14, 55
MlsI TGGCCA 2 cut(s) 594, 617
MluCI AATT 3 cut(s) 548, 582, 660
MluNI TGGCCA 2 cut(s) 594, 617
MlyI GAGTC 2 cut(s) 198, 221
MmeI TCCRAC 1 cut(s) 118
Mox20I TGGCCA 2 cut(s) 594, 617
Mph1103I ATGCAT 1 cut(s) 466
MscI TGGCCA 2 cut(s) 594, 617
MseI TTAA 3 cut(s) 270, 399, 672
Msp20I TGGCCA 2 cut(s) 594, 617
MspA1I CMGCKG 1 cut(s) 131
MspR9I CCNGG 3 cut(s) 19, 24, 602
Mva1269I GAATGC 2 cut(s) 346, 466
MvaI CCWGG 3 cut(s) 19, 24, 602
MwoI GCNNNNNNNGC 7 cut(s) 182, 329, 350, 371, 380, 389, 410
NdeII GATC 6 cut(s) 140, 199, 217, 311, 534, 636
NlaIII CATG 1 cut(s) 622
NlaIV GGNNCC 1 cut(s) 92
NmeAIII GCCGAG 2 cut(s) 418, 457
NsiI ATGCAT 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 376
PctI GAATGC 2 cut(s) 346, 466
PfeI GAWTC 1 cut(s) 306
PleI GAGTC 2 cut(s) 198, 221
PmaCI CACGTG 1 cut(s) 48
PmlI CACGTG 1 cut(s) 48
PpsI GAGTC 2 cut(s) 198, 221
Ppu21I YACGTR 1 cut(s) 48
PshBI ATTAAT 1 cut(s) 672
Psp124BI GAGCTC 1 cut(s) 14
Psp6I CCWGG 3 cut(s) 17, 22, 600
PspCI CACGTG 1 cut(s) 48
PspGI CCWGG 3 cut(s) 17, 22, 600
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 131
SacI GAGCTC 1 cut(s) 14
SaqAI TTAA 3 cut(s) 270, 399, 672
Sau3AI GATC 6 cut(s) 140, 199, 217, 311, 534, 636
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 2 cut(s) 198, 221
ScrFI CCNGG 3 cut(s) 19, 24, 602
SduI GDGCHC 2 cut(s) 14, 55
SfaNI GCATC 2 cut(s) 37, 418
SinI GGWCC 1 cut(s) 91
SmlI CTYRAG 2 cut(s) 485, 499
SmoI CTYRAG 2 cut(s) 485, 499
Sse9I AATT 3 cut(s) 548, 582, 660
SsiI CCGC 2 cut(s) 404, 413
SspMI CTAG 3 cut(s) 209, 245, 294
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 17, 22, 600
StyI CCWWGG 2 cut(s) 94, 678
TaiI ACGT 1 cut(s) 50
TaqI TCGA 6 cut(s) 34, 63, 202, 314, 516, 537
TasI AATT 3 cut(s) 548, 582, 660
TfiI GAWTC 1 cut(s) 306
Tru1I TTAA 3 cut(s) 270, 399, 672
Tru9I TTAA 3 cut(s) 270, 399, 672
TscAI CASTG 1 cut(s) 618
TspDTI ATGAA 4 cut(s) 371, 437, 473, 537
TspGWI ACGGA 2 cut(s) 205, 296
TspRI CASTG 1 cut(s) 618
VpaK11BI GGWCC 1 cut(s) 91
VspI ATTAAT 1 cut(s) 672
XapI RAATTY 1 cut(s) 548
XspI CTAG 3 cut(s) 209, 245, 294
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.