Rorug04G0204300

Flotillin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0018894
Physical Location & Seq
Forward (+)
35535877 .. 35536812
936 bp
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UTR
Exon/CDS
Intron
Rorug04G0204300.1

Sequence Viewer

Length: 474 bp
ATGTTTGCAGGCCATGAGGTCCATGTTATGGGGACGCCTGGTCATACCAAAGGTCAGATCAGCTTCTACTTTCCTGTGTCAGGGTCAATATTTACAGGAGACACTTTGTTCAGCCTATCATGTGGTAAGTTGTTTGAAGGAACACCTGACCAAATGCAGTATTCCCTTACAAAGATCATGTCTTTGCCAGATGATATAGAAGTATACTCTGGTCATAAATATACATTGAGTAACTCTAAGTTTGCCTTATCTATTGAACTTGAGAATGAAGCACTTCAGTCCTACGCAGCCCGTGTCGCACATCTTCGCAGCAAAGGGGTGCCTACGGTTCCTACCACATTGTTACTGGAGAAGGCTTGCAATCCTTTTCTTCGCACATCCAGTACAGAGATCCGGCAGTCGTTAAATATTTCAGCCTCAGCAAGTAAGGCGGAAGCACTGGGTGTCATACGCCAAGCCAAGGATACTTTCTGA

Protein Analysis

157

Amino Acids

17.11

Weight (kDa)

6.96

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 3 - 72 5.8e-08 Metallo-beta-lactamase superfamily
HAGH_C PF16123 74 - 157 2.5e-24 Hydroxyacylglutathione hydrolase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018894)

Species Orthologous Gene IDs
malus_domestica MD16G1287500.v1.1
rosa_chinensis RchiOBHm_Chr1g0356261
rosa_multiflora Rmu_co8276241.1_g000001
rosa_roxburghii Rroxscaffold_5G00367560 Rroxscaffold_7G00170660
rosa_rugosa Rorug04G0204300
rosa_samantha Rh4AG261600 Rh4BG267500 Rh4CG278600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 319
AccB7I CCANNNNNTGG 1 cut(s) 28
AccI GTMKAC 1 cut(s) 204
AciI CCGC 1 cut(s) 431
AclWI GGATC 1 cut(s) 385
AcuI CTGAAG 1 cut(s) 260
AcyI GRCGYC 1 cut(s) 35
AdeI CACNNNGTG 1 cut(s) 443
AfaI GTAC 1 cut(s) 385
AfiI CCNNNNNNNGG 3 cut(s) 28, 80, 460
AgsI TTSAA 2 cut(s) 137, 257
AhdI GACNNNNNGTC 1 cut(s) 39
AjnI CCWGG 1 cut(s) 37
AjuI GAANNNNNNNTTGG 1 cut(s) 452
AluBI AGCT 1 cut(s) 63
AluI AGCT 1 cut(s) 63
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 1 cut(s) 385
AoxI GGCC 1 cut(s) 10
ApeKI GCWGC 2 cut(s) 287, 309
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
Asp700I GAANNNNTTC 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 19
AvaII GGWCC 1 cut(s) 19
BanI GGYRCC 1 cut(s) 319
BbvCI CCTCAGC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 299, 321
BciT130I CCWGG 1 cut(s) 39
BciVI GTATCC 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 93
BfuI GTATCC 1 cut(s) 457
BisI GCNGC 2 cut(s) 288, 310
BlsI GCNGC 2 cut(s) 289, 311
Bme1390I CCNGG 1 cut(s) 39
Bme18I GGWCC 1 cut(s) 19
BmeRI GACNNNNNGTC 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 19
BmiI GGNNCC 2 cut(s) 321, 330
BmrFI CCNGG 1 cut(s) 39
BmrI ACTGGG 1 cut(s) 449
BmuI ACTGGG 1 cut(s) 449
BpmI CTGGAG 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 418
BpuEI CTTGAG 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 459
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 80, 460
Bse1I ACTGG 3 cut(s) 351, 381, 444
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 1 cut(s) 377
BseLI CCNNNNNNNGG 3 cut(s) 28, 80, 460
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 3 cut(s) 351, 381, 444
BseXI GCAGC 2 cut(s) 299, 321
BshFI GGCC 1 cut(s) 12
BshNI GGYRCC 1 cut(s) 319
BsiSI CCGG 1 cut(s) 394
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 3 cut(s) 28, 80, 460
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 46
BsnI GGCC 1 cut(s) 12
Bsp143I GATC 3 cut(s) 57, 174, 390
BspACI CCGC 1 cut(s) 431
BspANI GGCC 1 cut(s) 12
BspCNI CTCAG 1 cut(s) 431
BspLI GGNNCC 2 cut(s) 321, 330
BspPI GGATC 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 319
BsrI ACTGG 3 cut(s) 351, 381, 444
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 3 cut(s) 57, 174, 390
BssNAI GTATAC 1 cut(s) 205
BssNI GRCGYC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 459
Bst1107I GTATAC 1 cut(s) 205
Bst2UI CCWGG 1 cut(s) 39
Bst4CI ACNGT 1 cut(s) 328
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 10, 358
BstDEI CTNAG 2 cut(s) 237, 418
BstENI CCTNNNNNAGG 1 cut(s) 78
BstF5I GGATG 1 cut(s) 377
BstKTI GATC 3 cut(s) 60, 177, 393
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 3 cut(s) 57, 174, 390
BstMWI GCNNNNNNNGC 2 cut(s) 296, 428
BstNI CCWGG 1 cut(s) 39
BstSCI CCNGG 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 299, 321
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
BstZ17I GTATAC 1 cut(s) 205
BsuI GTATCC 1 cut(s) 457
BsuRI GGCC 1 cut(s) 12
BtsCI GGATG 1 cut(s) 377
BtsIMutI CAGTG 1 cut(s) 437
Cac8I GCNNGC 2 cut(s) 10, 358
Cfr13I GGNCC 1 cut(s) 19
CseI GACGC 1 cut(s) 43
Csp6I GTAC 1 cut(s) 384
CviAII CATG 4 cut(s) 14, 23, 120, 178
CviJI RGCY 7 cut(s) 12, 63, 114, 290, 356, 416, 458
CviKI_1 RGCY 7 cut(s) 12, 63, 114, 290, 356, 416, 458
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 2 cut(s) 237, 418
DpnI GATC 3 cut(s) 59, 176, 392
DpnII GATC 3 cut(s) 57, 174, 390
DraIII CACNNNGTG 1 cut(s) 443
DriI GACNNNNNGTC 1 cut(s) 39
Eam1105I GACNNNNNGTC 1 cut(s) 39
EciI GGCGGA 1 cut(s) 446
Eco130I CCWWGG 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 260
EcoNI CCTNNNNNAGG 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 4 cut(s) 17, 26, 123, 181
FalI AAGNNNNNCTT 2 cut(s) 230, 262
FaqI GGGAC 1 cut(s) 46
FatI CATG 4 cut(s) 13, 22, 119, 177
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 288, 310
FokI GGATG 1 cut(s) 364
Fsp4HI GCNGC 2 cut(s) 288, 310
GluI GCNGC 2 cut(s) 288, 310
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 1 cut(s) 12
HapII CCGG 1 cut(s) 394
HgaI GACGC 1 cut(s) 43
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 4 cut(s) 17, 26, 123, 181
HpaII CCGG 1 cut(s) 394
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 2 cut(s) 57, 473
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 2 cut(s) 131, 346
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4V TGCA 3 cut(s) 8, 157, 360
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 428
HpyF3I CTNAG 2 cut(s) 237, 418
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 4 cut(s) 17, 26, 123, 181
Kzo9I GATC 3 cut(s) 57, 174, 390
Lsp1109I GCAGC 2 cut(s) 299, 321
MaeIII GTNAC 2 cut(s) 230, 342
MalI GATC 3 cut(s) 59, 176, 392
MboI GATC 3 cut(s) 57, 174, 390
MboII GAAGA 2 cut(s) 296, 362
MflI RGATCY 1 cut(s) 390
MnlI CCTC 2 cut(s) 10, 427
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 1 cut(s) 404
MspI CCGG 1 cut(s) 394
MspR9I CCNGG 1 cut(s) 39
MvaI CCWGG 1 cut(s) 39
MwoI GCNNNNNNNGC 2 cut(s) 296, 428
NdeII GATC 3 cut(s) 57, 174, 390
NlaIII CATG 4 cut(s) 17, 26, 123, 181
NlaIV GGNNCC 2 cut(s) 321, 330
PdmI GAANNNNTTC 1 cut(s) 273
PflMI CCANNNNNTGG 1 cut(s) 28
PkrI GCNGC 2 cut(s) 289, 311
Psp6I CCWGG 1 cut(s) 37
PspGI CCWGG 1 cut(s) 37
PspN4I GGNNCC 2 cut(s) 321, 330
PspPI GGNCC 1 cut(s) 19
PsuI RGATCY 1 cut(s) 390
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 2 cut(s) 288, 310
Sau3AI GATC 3 cut(s) 57, 174, 390
Sau96I GGNCC 1 cut(s) 19
ScrFI CCNGG 1 cut(s) 39
SetI ASST 4 cut(s) 21, 55, 65, 148
SinI GGWCC 1 cut(s) 19
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
SsiI CCGC 1 cut(s) 431
SspI AATATT 2 cut(s) 90, 409
StyD4I CCNGG 1 cut(s) 37
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 1 cut(s) 328
TatI WGTACW 1 cut(s) 383
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TscAI CASTG 1 cut(s) 444
TseI GCWGC 2 cut(s) 287, 309
TspDTI ATGAA 1 cut(s) 282
TspRI CASTG 1 cut(s) 444
Van91I CCANNNNNTGG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 19
XagI CCTNNNNNAGG 1 cut(s) 78
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.