Rroxscaffold_5G00369080

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0018835
Physical Location & Seq
Forward (+)
50921129 .. 50921632
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369080.1

Sequence Viewer

Length: 504 bp
ATGAAGAAATCAATATCTCTAATAGTGGTACTGATACTTGTGATTCAAACTACCTTTGTTCCACTGAGTCACTGCAATGATCTTGTTGACGACACATGCAAACAGACACCACACCCCAACCTCTGTGTCTCCATTCTCAATGCAGACCCGCGGACTGAAGGGTCTGATGTGGATGGGTTAGCCACCATAATGGTTGGTGAAGTTGAGCCTAAGGCGTCTGATGCCATTGCCAAAATCAAACAGCTACTTAACCAGAGCTCTGCAGACAAACCCTTAAGCGATTGTCAGGACTATTATGTTGCAGTTGTTCGAGATAATGTGCCCCAAGCCACTGAAGCAATTGATGCAAGTGATGGTGGCAAGGCGGAGCAACTCATGAATGACGTTGTGGGTAAGGCTGATTCATGCGAAAAGGGTTTTAAGAGCGGCTCTCCTCTGACTAGTTTGAACAATGCCGTAAAAGATGTTTCGTCTGTGGCTGCAGTAATATCGAGATTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

167

Amino Acids

17.67

Weight (kDa)

4.71

Isoelectric Point (pI)

25.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 27 - 161 1.5e-20 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018835)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20430
rosa_multiflora Rmu_sc0000603.1_g000004 Rmu_ssc0000351.1_g000004
rosa_roxburghii Rroxscaffold_5G00344630 Rroxscaffold_5G00369080
rosa_rugosa Rorug04G0018300
rosa_samantha Rh2BG663500 Rh4CG294800 Rh4DG277000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 160
AccBSI CCGCTC 1 cut(s) 426
AccII CGCG 1 cut(s) 151
AciI CCGC 4 cut(s) 149, 151, 365, 426
AcuI CTGAAG 2 cut(s) 177, 354
AcyI GRCGYC 1 cut(s) 215
AfaI GTAC 1 cut(s) 30
AflII CTTAAG 1 cut(s) 274
AgsI TTSAA 2 cut(s) 47, 448
AhlI ACTAGT 1 cut(s) 440
AluBI AGCT 2 cut(s) 244, 258
AluI AGCT 2 cut(s) 244, 258
Alw21I GWGCWC 1 cut(s) 260
Alw26I GTCTC 1 cut(s) 133
ApeKI GCWGC 1 cut(s) 479
AseI ATTAAT 1 cut(s) 497
AsuHPI GGTGA 1 cut(s) 209
AxyI CCTNAGG 1 cut(s) 210
BaeGI GKGCMC 1 cut(s) 324
BanII GRGCYC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 260
BbvI GCAGC 1 cut(s) 466
BccI CCATC 2 cut(s) 167, 347
BceAI ACGGC 1 cut(s) 440
BcgI CGANNNNNNTGC 1 cut(s) 471
BcoDI GTCTC 1 cut(s) 133
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 1 cut(s) 441
BfmI CTRYAG 2 cut(s) 261, 480
BfrI CTTAAG 1 cut(s) 274
BisI GCNGC 2 cut(s) 427, 480
BlsI GCNGC 2 cut(s) 428, 481
BmsI GCATC 2 cut(s) 211, 334
BplI GAGNNNNNCTC 2 cut(s) 415, 447
BsaHI GRCGYC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 149
Bse21I CCTNAGG 1 cut(s) 210
Bse3DI GCAATG 2 cut(s) 82, 225
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 1 cut(s) 178
BseMI GCAATG 2 cut(s) 82, 225
BseMII CTCAG 1 cut(s) 56
BseRI GAGGAG 1 cut(s) 423
BseSI GKGCMC 1 cut(s) 324
BseXI GCAGC 1 cut(s) 466
Bsh1236I CGCG 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 260
BsmAI GTCTC 1 cut(s) 133
Bsp1286I GDGCHC 2 cut(s) 260, 324
Bsp143I GATC 1 cut(s) 79
BspACI CCGC 4 cut(s) 149, 151, 365, 426
BspCNI CTCAG 1 cut(s) 57
BspFNI CGCG 1 cut(s) 151
BspHI TCATGA 1 cut(s) 375
BspMAI CTGCAG 2 cut(s) 265, 484
BspTI CTTAAG 1 cut(s) 274
BsrBI CCGCTC 1 cut(s) 426
BsrDI GCAATG 2 cut(s) 82, 225
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 1 cut(s) 79
BssNI GRCGYC 1 cut(s) 215
BstACI GRCGYC 1 cut(s) 215
BstAFI CTTAAG 1 cut(s) 274
BstAPI GCANNNNNTGC 1 cut(s) 344
BstDEI CTNAG 2 cut(s) 65, 210
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 151
BstKTI GATC 1 cut(s) 82
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 1 cut(s) 79
BstMWI GCNNNNNNNGC 3 cut(s) 221, 335, 344
BstNSI RCATGY 1 cut(s) 99
BstSFI CTRYAG 2 cut(s) 261, 480
BstSLI GKGCMC 1 cut(s) 324
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 466
BstXI CCANNNNNNTGG 1 cut(s) 190
Bsu36I CCTNAGG 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 3 cut(s) 62, 70, 330
CciI TCATGA 1 cut(s) 375
Cfr42I CCGCGG 1 cut(s) 152
CseI GACGC 1 cut(s) 204
Csp6I GTAC 1 cut(s) 29
CviAII CATG 3 cut(s) 96, 376, 405
CviJI RGCY 8 cut(s) 182, 208, 244, 258, 329, 398, 429, 479
CviKI_1 RGCY 8 cut(s) 182, 208, 244, 258, 329, 398, 429, 479
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 2 cut(s) 65, 210
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
DrdI GACNNNNNNGTC 1 cut(s) 160
DseDI GACNNNNNNGTC 1 cut(s) 160
EciI GGCGGA 1 cut(s) 380
Ecl136II GAGCTC 1 cut(s) 258
Eco24I GRGCYC 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 258
Eco57I CTGAAG 2 cut(s) 177, 354
Eco81I CCTNAGG 1 cut(s) 210
EcoICRI GAGCTC 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 260
FaeI CATG 3 cut(s) 99, 379, 408
FaiI YATR 5 cut(s) 97, 188, 297, 377, 406
FatI CATG 3 cut(s) 95, 375, 404
FauI CCCGC 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 427, 480
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 1 cut(s) 260
Fsp4HI GCNGC 2 cut(s) 427, 480
FspBI CTAG 1 cut(s) 441
GluI GCNGC 2 cut(s) 427, 480
HgaI GACGC 1 cut(s) 204
Hin1I GRCGYC 1 cut(s) 215
Hin1II CATG 3 cut(s) 99, 379, 408
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HinfI GANTC 3 cut(s) 43, 67, 401
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 3 cut(s) 166, 220, 438
Hpy188III TCNNGA 4 cut(s) 287, 311, 376, 492
Hpy8I GTNNAC 1 cut(s) 88
HpyAV CCTTC 1 cut(s) 152
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 7 cut(s) 75, 99, 143, 263, 302, 347, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 335, 344
HpyF3I CTNAG 2 cut(s) 65, 210
HpySE526I ACGT 1 cut(s) 384
Hsp92I GRCGYC 1 cut(s) 215
Hsp92II CATG 3 cut(s) 99, 379, 408
KspI CCGCGG 1 cut(s) 152
Kzo9I GATC 1 cut(s) 79
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 2 cut(s) 266, 272
Lsp1109I GCAGC 1 cut(s) 466
LweI GCATC 2 cut(s) 211, 334
MaeI CTAG 1 cut(s) 441
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 1 cut(s) 68
MalI GATC 1 cut(s) 81
MbiI CCGCTC 1 cut(s) 426
MboI GATC 1 cut(s) 79
MboII GAAGA 1 cut(s) 16
MfeI CAATTG 1 cut(s) 339
MhlI GDGCHC 2 cut(s) 260, 324
MluCI AATT 2 cut(s) 339, 498
MlyI GAGTC 1 cut(s) 76
MnlI CCTC 2 cut(s) 131, 444
MseI TTAA 4 cut(s) 249, 275, 420, 497
MslI CAYNNNNRTG 2 cut(s) 75, 188
MspA1I CMGCKG 1 cut(s) 151
MspCI CTTAAG 1 cut(s) 274
MunI CAATTG 1 cut(s) 339
MvnI CGCG 1 cut(s) 151
MwoI GCNNNNNNNGC 3 cut(s) 221, 335, 344
NdeII GATC 1 cut(s) 79
NlaIII CATG 3 cut(s) 99, 379, 408
NmuCI GTSAC 1 cut(s) 68
NspI RCATGY 1 cut(s) 99
PagI TCATGA 1 cut(s) 375
PfeI GAWTC 2 cut(s) 43, 401
PkrI GCNGC 2 cut(s) 428, 481
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
PshBI ATTAAT 1 cut(s) 497
Psp124BI GAGCTC 1 cut(s) 260
PstI CTGCAG 2 cut(s) 265, 484
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
RseI CAYNNNNRTG 2 cut(s) 75, 188
SacI GAGCTC 1 cut(s) 260
SacII CCGCGG 1 cut(s) 152
SaqAI TTAA 4 cut(s) 249, 275, 420, 497
SatI GCNGC 2 cut(s) 427, 480
Sau3AI GATC 1 cut(s) 79
SchI GAGTC 1 cut(s) 76
SduI GDGCHC 2 cut(s) 260, 324
SetI ASST 5 cut(s) 56, 123, 246, 260, 387
SfaNI GCATC 2 cut(s) 211, 334
SfcI CTRYAG 2 cut(s) 261, 480
Sfr303I CCGCGG 1 cut(s) 152
SgrBI CCGCGG 1 cut(s) 152
SmiMI CAYNNNNRTG 2 cut(s) 75, 188
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
SpeI ACTAGT 1 cut(s) 440
Sse9I AATT 2 cut(s) 339, 498
SsiI CCGC 4 cut(s) 149, 151, 365, 426
SspMI CTAG 1 cut(s) 441
SstI GAGCTC 1 cut(s) 260
TaiI ACGT 1 cut(s) 387
TaqI TCGA 2 cut(s) 310, 491
TasI AATT 2 cut(s) 339, 498
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 2 cut(s) 43, 401
Tru1I TTAA 4 cut(s) 249, 275, 420, 497
Tru9I TTAA 4 cut(s) 249, 275, 420, 497
TscAI CASTG 3 cut(s) 69, 77, 337
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 3 cut(s) 17, 392, 393
TspRI CASTG 3 cut(s) 69, 77, 337
Vha464I CTTAAG 1 cut(s) 274
VspI ATTAAT 1 cut(s) 497
XceI RCATGY 1 cut(s) 99
XspI CTAG 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.