Rh4CG294800

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018835
Physical Location & Seq
Forward (+)
55404352 .. 55404771
420 bp
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UTR
Exon/CDS
Intron
Rh4CG294800.1

Sequence Viewer

Length: 420 bp
ATGATCTTGTTGACGACACATGCAAACAGACACCACACCCCGACCTCTGTATCTCCATTCTCAATGCAGACCGGCGGACTGAAGGGTCTGATGCATGTGGATGGGTTAGCCACCATAATGGTTGGTGAAGTTGAGCCTAAGGCATCTGATGCCATTGCCAAAATCAAACAGCTACTTAACCAGAGCCTTGCAGACAAACCATTAAGCGATTGTCAGGAATATTATGTTATAGTTGTTCGAGATAATGTGCCCCAAGCCACTGAAGCAATTGGTGCAAGTGATGGTGGCAAGGCAGAGCAACTCATGAATGATGTTGTGGGTAAGGCTGATTCATGCGAAAAAGGGTTTAAGAGCTCGCCCCTAAACAATGCCGTAAAAGATGTTTCGTTTGTGGCTGCAGCAATATCGAGATTAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

139

Amino Acids

14.72

Weight (kDa)

6.04

Isoelectric Point (pI)

32.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 127 2.3e-08 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0018835)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20430
rosa_multiflora Rmu_sc0000603.1_g000004 Rmu_ssc0000351.1_g000004
rosa_roxburghii Rroxscaffold_5G00344630 Rroxscaffold_5G00369080
rosa_rugosa Rorug04G0018300
rosa_samantha Rh2BG663500 Rh4CG294800 Rh4DG277000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 84
AciI CCGC 1 cut(s) 75
AcuI CTGAAG 2 cut(s) 101, 282
AluBI AGCT 2 cut(s) 172, 354
AluI AGCT 2 cut(s) 172, 354
Alw21I GWGCWC 1 cut(s) 356
ApeKI GCWGC 2 cut(s) 395, 398
AsuHPI GGTGA 1 cut(s) 137
AxyI CCTNAGG 1 cut(s) 138
BaeGI GKGCMC 1 cut(s) 252
BaeI ACNNNNGTAYC 2 cut(s) 33, 66
BanII GRGCYC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 356
BbvI GCAGC 2 cut(s) 382, 410
BccI CCATC 2 cut(s) 95, 275
BceAI ACGGC 1 cut(s) 356
BcgI CGANNNNNNTGC 1 cut(s) 387
BfmI CTRYAG 1 cut(s) 396
BisI GCNGC 2 cut(s) 396, 399
BlsI GCNGC 2 cut(s) 397, 400
BmsI GCATC 3 cut(s) 81, 139, 152
Bse118I RCCGGY 1 cut(s) 71
Bse21I CCTNAGG 1 cut(s) 138
Bse3DI GCAATG 1 cut(s) 153
BseGI GGATG 1 cut(s) 106
BseMI GCAATG 1 cut(s) 153
BseSI GKGCMC 1 cut(s) 252
BseXI GCAGC 2 cut(s) 382, 410
BsiHKAI GWGCWC 1 cut(s) 356
BsiSI CCGG 1 cut(s) 72
Bsp1286I GDGCHC 2 cut(s) 252, 356
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 1 cut(s) 75
BspHI TCATGA 1 cut(s) 303
BspMAI CTGCAG 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 71
BssMI GATC 1 cut(s) 3
BstAPI GCANNNNNTGC 2 cut(s) 149, 272
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 106
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 3 cut(s) 149, 263, 272
BstNSI RCATGY 2 cut(s) 23, 98
BstSFI CTRYAG 1 cut(s) 396
BstSLI GKGCMC 1 cut(s) 252
BstV1I GCAGC 2 cut(s) 382, 410
BstXI CCANNNNNNTGG 1 cut(s) 118
Bsu36I CCTNAGG 1 cut(s) 138
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 356
CciI TCATGA 1 cut(s) 303
Cfr10I RCCGGY 1 cut(s) 71
CviAII CATG 4 cut(s) 20, 95, 304, 333
CviJI RGCY 8 cut(s) 110, 136, 172, 186, 257, 326, 354, 395
CviKI_1 RGCY 8 cut(s) 110, 136, 172, 186, 257, 326, 354, 395
DdeI CTNAG 1 cut(s) 138
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
DrdI GACNNNNNNGTC 1 cut(s) 84
DseDI GACNNNNNNGTC 1 cut(s) 84
EciI GGCGGA 1 cut(s) 90
Ecl136II GAGCTC 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 356
Eco53kI GAGCTC 1 cut(s) 354
Eco57I CTGAAG 2 cut(s) 101, 282
Eco81I CCTNAGG 1 cut(s) 138
EcoICRI GAGCTC 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 96
EcoT38I GRGCYC 1 cut(s) 356
FaeI CATG 4 cut(s) 23, 98, 307, 336
FaiI YATR 7 cut(s) 21, 96, 116, 225, 230, 305, 334
FatI CATG 4 cut(s) 19, 94, 303, 332
Fnu4HI GCNGC 2 cut(s) 396, 399
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 1 cut(s) 356
Fsp4HI GCNGC 2 cut(s) 396, 399
GluI GCNGC 2 cut(s) 396, 399
HapII CCGG 1 cut(s) 72
Hin1II CATG 4 cut(s) 23, 98, 307, 336
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 1 cut(s) 329
HpaII CCGG 1 cut(s) 72
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 2 cut(s) 90, 148
Hpy188III TCNNGA 4 cut(s) 215, 239, 304, 408
Hpy8I GTNNAC 1 cut(s) 12
HpyAV CCTTC 1 cut(s) 76
HpyCH4V TGCA 6 cut(s) 23, 67, 94, 191, 275, 398
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 263, 272
HpyF3I CTNAG 1 cut(s) 138
Hsp92II CATG 4 cut(s) 23, 98, 307, 336
Kzo9I GATC 1 cut(s) 3
LpnPI CCDG 3 cut(s) 85, 194, 200
Lsp1109I GCAGC 2 cut(s) 382, 410
LweI GCATC 3 cut(s) 81, 139, 152
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MfeI CAATTG 1 cut(s) 267
MhlI GDGCHC 2 cut(s) 252, 356
MluCI AATT 1 cut(s) 267
MnlI CCTC 1 cut(s) 55
Mph1103I ATGCAT 1 cut(s) 96
MseI TTAA 4 cut(s) 177, 203, 348, 413
MslI CAYNNNNRTG 2 cut(s) 99, 116
MspI CCGG 1 cut(s) 72
MunI CAATTG 1 cut(s) 267
MwoI GCNNNNNNNGC 3 cut(s) 149, 263, 272
NdeII GATC 1 cut(s) 3
NlaIII CATG 4 cut(s) 23, 98, 307, 336
NsiI ATGCAT 1 cut(s) 96
NspI RCATGY 2 cut(s) 23, 98
PagI TCATGA 1 cut(s) 303
PfeI GAWTC 1 cut(s) 329
PkrI GCNGC 2 cut(s) 397, 400
Psp124BI GAGCTC 1 cut(s) 356
PstI CTGCAG 1 cut(s) 400
RseI CAYNNNNRTG 2 cut(s) 99, 116
SacI GAGCTC 1 cut(s) 356
SaqAI TTAA 4 cut(s) 177, 203, 348, 413
SatI GCNGC 2 cut(s) 396, 399
Sau3AI GATC 1 cut(s) 3
SduI GDGCHC 2 cut(s) 252, 356
SetI ASST 3 cut(s) 47, 174, 356
SfaNI GCATC 3 cut(s) 81, 139, 152
SfcI CTRYAG 1 cut(s) 396
SmiMI CAYNNNNRTG 2 cut(s) 99, 116
Sse9I AATT 1 cut(s) 267
SsiI CCGC 1 cut(s) 75
SspI AATATT 1 cut(s) 221
SstI GAGCTC 1 cut(s) 356
TaqI TCGA 2 cut(s) 238, 407
TasI AATT 1 cut(s) 267
TfiI GAWTC 1 cut(s) 329
Tru1I TTAA 4 cut(s) 177, 203, 348, 413
Tru9I TTAA 4 cut(s) 177, 203, 348, 413
TscAI CASTG 1 cut(s) 265
TseI GCWGC 2 cut(s) 395, 398
TspDTI ATGAA 2 cut(s) 320, 321
TspRI CASTG 1 cut(s) 265
XceI RCATGY 2 cut(s) 23, 98
Zsp2I ATGCAT 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.