Rorug04G0018300

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0018835
Physical Location & Seq
Reverse (-)
2683462 .. 2689096
5635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0018300.1

Sequence Viewer

Length: 1035 bp
ATGGAGGACAAATCTGATATTGAAATCCCAAAGGTTAAACTGGGCAGTCAAGGCTTGGAGGTGTCTAGATTGGGTTTTGGATGTGGGGGACTATCTGGAATATACAATGCTCCTCTCTCTCACGAAGCTGGATGCTCAGTGATACAGGAAGCATTCAATAGGGGCATCACCTTCTATGATACCTCAGATGCTTATGGTCAGGATCATGATAATGAAATCATGGTCGGCAAGGCTTTAAAACAACTTCCTCGAGAAAAGGTTCAATTGGCTACAAAATTTGGCATTCTCAAGTCAGACAGCTTTGAATTCAGTGTAAATGGGACCCCGGAGTACGTAAGAGCATGCTGTGAAGCTAGTCTGAAGAGGCTGGATGTCGACTACATTGATTTGTACTATCAGCACCGTGTCGATGTTTCAGTGCCAATTGAGGATACGATTGAGGAGCTTAAGAAGCTAGTGAATGAAGGAAAAATAAGATACATTGGCCTTTCAGAAGCTAGTATAGACACAATAATGAGAGCTCATGCTGTTCATCCCATTACTGCAGTGCAAATGGAGTATTCTCTTTGGACTCGTGAAATTGAAAATGAGATAATTCCACTTTGCAGGAAACTTGGAATTGGGATAGTGTCATATAGTCCACTTGGTCGTGGCTTCTTCGGTGGGAAGGCAATTTTAGAGAGCTTGCCTGCAGAAAGTTTGTTGTCTATTCATCCAAGGTTCAATGGCGAGAATTTGGAAAAGAACAAACTTTTCTATAACAAGCTTGCCAATCTTGCTGCAAAGCATTCATGCAATGCTCCTCAATTAGCTCTGGCATGGCTTCTCCATCAGGGCAAGGACATAATTCCAATCCCCGGGACTACTAAACTTAAGAACCTGGATATCAATGTCGGATCGTTGGATGTGAAGCTTACAGAAGAGGATTTGAATGAAATTTGTGAAGCTGTGCCCATTGATAAAGTTGGAGGAGACCGAGAATTTGAAGTGTTCTCAAAATTTGTGTGGAATTATGCAAGTACACCCTCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

344

Amino Acids

38.21

Weight (kDa)

5.32

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 315 4e-70 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018835)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20430
rosa_multiflora Rmu_sc0000603.1_g000004 Rmu_ssc0000351.1_g000004
rosa_roxburghii Rroxscaffold_5G00344630 Rroxscaffold_5G00369080
rosa_rugosa Rorug04G0018300
rosa_samantha Rh2BG663500 Rh4CG294800 Rh4DG277000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 375
AclWI GGATC 2 cut(s) 210, 904
AcsI RAATTY 6 cut(s) 275, 305, 733, 936, 980, 998
AcuI CTGAAG 1 cut(s) 380
AfaI GTAC 3 cut(s) 332, 392, 1021
AfiI CCNNNNNNNGG 1 cut(s) 857
AflII CTTAAG 2 cut(s) 446, 872
AgsI TTSAA 8 cut(s) 23, 157, 263, 305, 584, 724, 931, 986
AjnI CCWGG 1 cut(s) 879
Alw21I GWGCWC 1 cut(s) 523
Alw26I GTCTC 1 cut(s) 966
AlwI GGATC 2 cut(s) 210, 904
Ama87I CYCGRG 2 cut(s) 249, 857
AoxI GGCC 1 cut(s) 484
ApeKI GCWGC 1 cut(s) 779
ApoI RAATTY 6 cut(s) 275, 305, 733, 936, 980, 998
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
Asp700I GAANNNNTTC 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 321
AsuC2I CCSGG 3 cut(s) 326, 858, 859
AsuHPI GGTGA 1 cut(s) 160
AvaI CYCGRG 2 cut(s) 249, 857
AvaII GGWCC 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 954
BanII GRGCYC 1 cut(s) 523
BauI CACGAG 1 cut(s) 573
Bbv12I GWGCWC 1 cut(s) 523
BbvI GCAGC 1 cut(s) 766
BccI CCATC 1 cut(s) 837
BciT130I CCWGG 1 cut(s) 881
BciVI GTATCC 1 cut(s) 424
BcnI CCSGG 3 cut(s) 326, 858, 859
BcoDI GTCTC 1 cut(s) 966
BfaI CTAG 4 cut(s) 66, 354, 455, 498
BfmI CTRYAG 2 cut(s) 543, 690
BfrI CTTAAG 2 cut(s) 446, 872
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 1 cut(s) 780
BlsI GCNGC 1 cut(s) 781
Bme1390I CCNGG 4 cut(s) 326, 858, 859, 881
Bme18I GGWCC 1 cut(s) 321
BmeT110I CYCGRG 2 cut(s) 249, 857
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 2 cut(s) 322, 323
BmrFI CCNGG 4 cut(s) 326, 858, 859, 881
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 3 cut(s) 122, 174, 178
BmuI ACTGGG 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 3 cut(s) 326, 858, 859
BsaAI YACGTR 1 cut(s) 334
BsaI GGTCTC 1 cut(s) 966
BsaJI CCNNGG 4 cut(s) 324, 716, 856, 857
Bsc4I CCNNNNNNNGG 1 cut(s) 857
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 802
BseBI CCWGG 1 cut(s) 881
BseDI CCNNGG 4 cut(s) 324, 716, 856, 857
BseGI GGATG 6 cut(s) 86, 137, 376, 532, 712, 910
BseLI CCNNNNNNNGG 1 cut(s) 857
BseMI GCAATG 1 cut(s) 802
BseMII CTCAG 2 cut(s) 150, 198
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 4 cut(s) 102, 455, 792, 984
BseSI GKGCMC 1 cut(s) 954
BseXI GCAGC 1 cut(s) 766
BshFI GGCC 1 cut(s) 486
BsiHKAI GWGCWC 1 cut(s) 523
BsiHKCI CYCGRG 2 cut(s) 249, 857
BsiSI CCGG 2 cut(s) 326, 858
BslFI GGGAC 3 cut(s) 102, 334, 874
BslI CCNNNNNNNGG 1 cut(s) 857
BsmAI GTCTC 1 cut(s) 966
BsmFI GGGAC 3 cut(s) 102, 334, 874
BsmI GAATGC 3 cut(s) 152, 282, 787
BsnI GGCC 1 cut(s) 486
Bso31I GGTCTC 1 cut(s) 966
BsoBI CYCGRG 2 cut(s) 249, 857
Bsp1286I GDGCHC 2 cut(s) 523, 954
Bsp143I GATC 2 cut(s) 202, 896
BspANI GGCC 1 cut(s) 486
BspCNI CTCAG 2 cut(s) 149, 197
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 322, 323
BspMAI CTGCAG 2 cut(s) 547, 694
BspPI GGATC 2 cut(s) 210, 904
BspTI CTTAAG 2 cut(s) 446, 872
BspTNI GGTCTC 1 cut(s) 966
BsrDI GCAATG 1 cut(s) 802
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 4 cut(s) 324, 716, 856, 857
BssMI GATC 2 cut(s) 202, 896
BssSI CACGAG 1 cut(s) 573
BssT1I CCWWGG 1 cut(s) 716
Bst2BI CACGAG 1 cut(s) 573
Bst2UI CCWGG 1 cut(s) 881
Bst4CI ACNGT 1 cut(s) 404
Bst6I CTCTTC 2 cut(s) 356, 915
BstAFI CTTAAG 2 cut(s) 446, 872
BstBAI YACGTR 1 cut(s) 334
BstC8I GCNNGC 4 cut(s) 343, 686, 690, 768
BstDEI CTNAG 2 cut(s) 136, 184
BstF5I GGATG 6 cut(s) 86, 137, 376, 532, 712, 910
BstKTI GATC 2 cut(s) 205, 899
BstMAI GTCTC 1 cut(s) 966
BstMBI GATC 2 cut(s) 202, 896
BstMWI GCNNNNNNNGC 3 cut(s) 51, 451, 776
BstNI CCWGG 1 cut(s) 881
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 4 cut(s) 324, 856, 857, 879
BstSFI CTRYAG 2 cut(s) 543, 690
BstSLI GKGCMC 1 cut(s) 954
BstSNI TACGTA 1 cut(s) 334
BstV1I GCAGC 1 cut(s) 766
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 1 cut(s) 486
BtsCI GGATG 6 cut(s) 86, 137, 376, 532, 712, 910
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 4 cut(s) 144, 316, 423, 552
Cac8I GCNNGC 4 cut(s) 343, 686, 690, 768
CciI TCATGA 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 321
Cfr9I CCCGGG 1 cut(s) 857
Csp6I GTAC 3 cut(s) 331, 391, 1020
CviAII CATG 6 cut(s) 206, 220, 342, 524, 792, 819
CviQI GTAC 3 cut(s) 331, 391, 1020
DdeI CTNAG 2 cut(s) 136, 184
DpnI GATC 2 cut(s) 204, 898
DpnII GATC 2 cut(s) 202, 896
DraI TTTAAA 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 356, 915
EarI CTCTTC 2 cut(s) 356, 915
Ecl136II GAGCTC 1 cut(s) 521
Eco105I TACGTA 1 cut(s) 334
Eco130I CCWWGG 1 cut(s) 716
Eco24I GRGCYC 1 cut(s) 523
Eco31I GGTCTC 1 cut(s) 966
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 1 cut(s) 321
Eco53kI GAGCTC 1 cut(s) 521
Eco57I CTGAAG 1 cut(s) 380
Eco88I CYCGRG 2 cut(s) 249, 857
EcoICRI GAGCTC 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 321
EcoRI GAATTC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 879
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 1 cut(s) 716
EcoT38I GRGCYC 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 716
FaeI CATG 6 cut(s) 209, 223, 345, 527, 795, 822
FaqI GGGAC 3 cut(s) 102, 334, 874
FatI CATG 6 cut(s) 205, 219, 341, 523, 791, 818
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 1 cut(s) 780
FokI GGATG 6 cut(s) 93, 144, 383, 519, 699, 917
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 1 cut(s) 780
FspBI CTAG 4 cut(s) 66, 354, 455, 498
GluI GCNGC 1 cut(s) 780
HaeIII GGCC 1 cut(s) 486
HapII CCGG 2 cut(s) 326, 858
Hin1II CATG 6 cut(s) 209, 223, 345, 527, 795, 822
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 2 cut(s) 764, 911
HinfI GANTC 1 cut(s) 571
HpaII CCGG 2 cut(s) 326, 858
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 3 cut(s) 376, 641, 1022
Hpy188I TCNGA 6 cut(s) 16, 187, 295, 360, 493, 896
Hpy188III TCNNGA 7 cut(s) 66, 96, 122, 200, 206, 251, 575
Hpy8I GTNNAC 3 cut(s) 376, 641, 1022
HpyAV CCTTC 3 cut(s) 181, 458, 661
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 7 cut(s) 545, 550, 606, 692, 782, 795, 1016
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 451, 776
HpyF3I CTNAG 2 cut(s) 136, 184
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 6 cut(s) 209, 223, 345, 527, 795, 822
KflI GGGWCCC 1 cut(s) 321
Kzo9I GATC 2 cut(s) 202, 896
LmnI GCTCC 3 cut(s) 115, 442, 805
Lsp1109I GCAGC 1 cut(s) 766
LweI GCATC 3 cut(s) 122, 174, 178
MaeI CTAG 4 cut(s) 66, 354, 455, 498
MaeII ACGT 1 cut(s) 333
MalI GATC 2 cut(s) 204, 898
MboI GATC 2 cut(s) 202, 896
MboII GAAGA 3 cut(s) 373, 649, 932
MfeI CAATTG 2 cut(s) 263, 423
MhlI GDGCHC 2 cut(s) 523, 954
MlyI GAGTC 1 cut(s) 565
MmeI TCCRAC 3 cut(s) 874, 882, 946
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 4 cut(s) 36, 236, 447, 873
MslI CAYNNNNRTG 2 cut(s) 210, 512
MspCI CTTAAG 2 cut(s) 446, 872
MspI CCGG 2 cut(s) 326, 858
MspR9I CCNGG 4 cut(s) 326, 858, 859, 881
MunI CAATTG 2 cut(s) 263, 423
Mva1269I GAATGC 3 cut(s) 152, 282, 787
MvaI CCWGG 1 cut(s) 881
MwoI GCNNNNNNNGC 3 cut(s) 51, 451, 776
NciI CCSGG 3 cut(s) 326, 858, 859
NdeII GATC 2 cut(s) 202, 896
NlaIII CATG 6 cut(s) 209, 223, 345, 527, 795, 822
NlaIV GGNNCC 2 cut(s) 322, 323
NspI RCATGY 1 cut(s) 345
PaeI GCATGC 1 cut(s) 345
PaeR7I CTCGAG 1 cut(s) 249
PagI TCATGA 1 cut(s) 205
PctI GAATGC 3 cut(s) 152, 282, 787
PdmI GAANNNNTTC 1 cut(s) 258
PkrI GCNGC 1 cut(s) 781
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Ppu21I YACGTR 1 cut(s) 334
PpuMI RGGWCCY 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 879
PspGI CCWGG 1 cut(s) 879
PspN4I GGNNCC 2 cut(s) 322, 323
PspPI GGNCC 1 cut(s) 321
PspPPI RGGWCCY 1 cut(s) 321
PstI CTGCAG 2 cut(s) 547, 694
RsaI GTAC 3 cut(s) 332, 392, 1021
RsaNI GTAC 3 cut(s) 331, 391, 1020
RseI CAYNNNNRTG 2 cut(s) 210, 512
SacI GAGCTC 1 cut(s) 523
SalI GTCGAC 1 cut(s) 374
SaqAI TTAA 4 cut(s) 36, 236, 447, 873
SatI GCNGC 1 cut(s) 780
Sau3AI GATC 2 cut(s) 202, 896
Sau96I GGNCC 1 cut(s) 321
SchI GAGTC 1 cut(s) 565
ScrFI CCNGG 4 cut(s) 326, 858, 859, 881
SduI GDGCHC 2 cut(s) 523, 954
SfaNI GCATC 3 cut(s) 122, 174, 178
SfcI CTRYAG 2 cut(s) 543, 690
Sfr274I CTCGAG 1 cut(s) 249
SinI GGWCC 1 cut(s) 321
SlaI CTCGAG 1 cut(s) 249
SmaI CCCGGG 1 cut(s) 859
SmiMI CAYNNNNRTG 2 cut(s) 210, 512
SmlI CTYRAG 4 cut(s) 249, 287, 446, 872
SmoI CTYRAG 4 cut(s) 249, 287, 446, 872
SnaBI TACGTA 1 cut(s) 334
SphI GCATGC 1 cut(s) 345
SspMI CTAG 4 cut(s) 66, 354, 455, 498
SstI GAGCTC 1 cut(s) 523
StyD4I CCNGG 4 cut(s) 324, 856, 857, 879
StyI CCWWGG 1 cut(s) 716
TaaI ACNGT 1 cut(s) 404
TaiI ACGT 1 cut(s) 336
TaqI TCGA 3 cut(s) 250, 375, 408
TaqII GACCGA 1 cut(s) 990
TatI WGTACW 2 cut(s) 390, 1019
Tru1I TTAA 4 cut(s) 36, 236, 447, 873
Tru9I TTAA 4 cut(s) 36, 236, 447, 873
TscAI CASTG 4 cut(s) 144, 316, 423, 552
TseI GCWGC 1 cut(s) 779
TspDTI ATGAA 6 cut(s) 228, 477, 521, 701, 780, 948
TspMI CCCGGG 1 cut(s) 857
TspRI CASTG 4 cut(s) 144, 316, 423, 552
Vha464I CTTAAG 2 cut(s) 446, 872
VpaK11BI GGWCC 1 cut(s) 321
XapI RAATTY 6 cut(s) 275, 305, 733, 936, 980, 998
XbaI TCTAGA 1 cut(s) 65
XceI RCATGY 1 cut(s) 345
XhoI CTCGAG 1 cut(s) 249
XmaI CCCGGG 1 cut(s) 857
XmiI GTMKAC 1 cut(s) 375
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 4 cut(s) 66, 354, 455, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.