Rroxscaffold_5G00383940

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011673
Physical Location & Seq
Forward (+)
63507005 .. 63511077
4073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383940.1

Sequence Viewer

Length: 1629 bp
ATGGAAGTGAGGAAGGACTCCATGGCTGTTCTGCTCCTGCTTCTTCTTTTAGCTGTGTCTTCAGTTTCTGCTGCTGACGGGGAAAATGTGGGTAGAGAGTGTCTTGATTTCTTTGGTAGAGTGTATAGATTAGAAGAGGTCAATCTCTGCCATGTTGAAAGTCTCCGATCTGTTGTCAGCTTTGATGCTTTTGTGGGTATAAACCTTGGTACAGACCTCTCTGACATGCCAAGTCCAACTGAGGTGGTTGCTCTTCTTAAAGCTCAAAACATTCGACATATCAGGCTCTACGATGCAGACAGAGCCATGCTCCTTGCTCTTGCAAACACAGGCATCCAAGTAACTGTTTCTGTCCCCAATGATCAGCTCCTTGGGATAGGTCAGTCCAATGCCACCGCGGCCAACTGGATTACTCGCAATGTGATAGCACATGTTCCTGCCACAAACATAACAGCTATAGCTGTCGGATCTGAAGTCCTAACTGCCCTCCCAAATGCTGCTCCAGTGCTAGTCTCTGCCCTAAAGTTCATTCACTCAGCACTTGTTGCGTCTAACCTTGATCGCCAAATTAAAGTCTCTACTCCACACTCTTCCTCCATTATTCTTGACTCCTTTCCTCCTTCCCAAGCCTTCTTCAATCGCTCATGGGACAAGGTCATGGTTCCGGTGCTCAATTTTTTGCAGTCTACAGATTCATATTTCATGCTCAATGTCTACCCTTACTATGATTATATGCAATCAAACGGTGTGATCCCCTTGGACTATGCACTTTTCCGCCCTCTCCCTCCAAACAAGGAAGCTGTTGATGCTAATACATTGCTGCATTATACTAATGTCTTTGATGCAATTGTTGATGCCGCGTATTTTGCAATGTCCTATCTAAACATTACAAATCTCCCCATTGTAGTGACTGAGTCAGGCTGGCCCTCCAAGGGTGACTCAGCAGAGCCAGATGCCACACTTGACAATGCTAACACTTACAATAGTAACCTAATCAAGCATGTGCTTAACAACACTGGGACTCCTAAGCATCCTGGGATTGCTGTTAGTACTTACATTTATGAGCTCTACAATGAGGATTTGAGACCTGGATCAGTCTCCGAAAAGAACTGGGGACTTTTTGATGCAAATGGGTTGCCAGTTTATACCTTACACTTGACAGGTGCTGGAACTGTGTTGGCAAACGACACAACGAACCAAACCTTTTGTGTCGCGAAGGAGGGTGCTGACAAAAAGATGCTACAGGCAGCCCTAGATTGGGCTTGTGGACCTGGAAAAGTTGATTGCTCACCTATGCTGCAAGGGAAACCTTGTTATGAACCAGACAATGTGGTGACTCATGCAACATATGCTTTCAATGCATACTTTCAGAAGATGGCCAAGTCTCCTGGGACTTGTGATTTCAAAGGGGTAGCTACTATTACTACCACAGACCCAAGTCATGGTTCTTGTATAGTTCCTGGAAGTGCTGGAAGAAATGGCACATTTGATAATAGTACATCACTTGCTCCATCTTCAAATTCTACAACTGCTTCTTCTGGGTGCATTTCACTATTTTTCCACAGCAGTGGTTCCTTCGCAAGCTCTGCCATCATTGGTTTCTTAGTTCTAAGTGCAGTTTTCTTGTAG

Protein Analysis

542

Amino Acids

58.23

Weight (kDa)

5.18

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 65 - 385 1.9e-77 Glycosyl hydrolases family 17
X8 PF07983 402 - 473 9.3e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011673)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01630 AT2G01630 AT2G01630
fragaria_vesca FvH4_4g33870 FvH4_4g33870
malus_domestica MD13G1030200.v1.1 MD16G1021000.v1.1
prunus_persica Prupe.1G331300_v2.0.a1
pyrus_communis pycom13g01850 pycom16g01880
rosa_chinensis RchiOBHm_Chr4g0443381
rosa_laevigata RLG00000005934
rosa_roxburghii Rroxscaffold_5G00383940
rosa_rugosa Rorug04G0345800
rosa_samantha Rh4AG401100 Rh4BG412600 Rh4CG428000 Rh4DG408100
rosa_wichuraiana Rw4G034590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1442
AccI GTMKAC 2 cut(s) 686, 714
AccII CGCG 3 cut(s) 398, 860, 1214
AciI CCGC 4 cut(s) 396, 398, 775, 858
AclWI GGATC 3 cut(s) 475, 745, 1099
AcoI YGGCCR 2 cut(s) 399, 1377
AcsI RAATTY 1 cut(s) 1519
AcuI CTGAAG 2 cut(s) 45, 492
AfaI GTAC 3 cut(s) 211, 1051, 1498
AfiI CCNNNNNNNGG 4 cut(s) 932, 1257, 1258, 1442
AflIII ACRYGT 1 cut(s) 430
AgsI TTSAA 5 cut(s) 158, 637, 1357, 1405, 1518
AjnI CCWGG 5 cut(s) 1033, 1087, 1270, 1387, 1459
AjuI GAANNNNNNNTTGG 2 cut(s) 1429, 1461
AleI CACNNNNGTG 1 cut(s) 1566
Alw21I GWGCWC 2 cut(s) 672, 1068
Alw26I GTCTC 6 cut(s) 167, 517, 580, 1078, 1102, 1389
AlwI GGATC 3 cut(s) 475, 745, 1099
AlwNI CAGNNNCTG 2 cut(s) 68, 1166
AoxI GGCC 3 cut(s) 399, 923, 1377
ApeKI GCWGC 5 cut(s) 71, 497, 820, 1247, 1297
ApoI RAATTY 1 cut(s) 1519
AspS9I GGNCC 2 cut(s) 924, 1268
AsuHPI GGTGA 3 cut(s) 947, 1281, 1345
AvaII GGWCC 1 cut(s) 1268
BalI TGGCCA 1 cut(s) 1379
BanII GRGCYC 1 cut(s) 1068
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 2 cut(s) 672, 1068
BbvI GCAGC 5 cut(s) 58, 484, 807, 1259, 1284
BccI CCATC 3 cut(s) 1369, 1519, 1598
BciT130I CCWGG 5 cut(s) 1035, 1089, 1272, 1389, 1461
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 6 cut(s) 167, 517, 580, 1078, 1102, 1389
BfaI CTAG 2 cut(s) 509, 1253
BfmI CTRYAG 3 cut(s) 456, 687, 1241
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 7 cut(s) 72, 399, 498, 821, 858, 1248, 1298
BlsI GCNGC 7 cut(s) 73, 400, 499, 822, 859, 1249, 1299
BmcAI AGTACT 1 cut(s) 1051
Bme1390I CCNGG 5 cut(s) 1035, 1089, 1272, 1389, 1461
Bme18I GGWCC 1 cut(s) 1268
BmgT120I GGNCC 2 cut(s) 924, 1268
BmiI GGNNCC 2 cut(s) 663, 1573
BmrFI CCNGG 5 cut(s) 1035, 1089, 1272, 1389, 1461
BmrI ACTGGG 2 cut(s) 1026, 1120
BmuI ACTGGG 2 cut(s) 1026, 1120
BoxI GACNNNNGTC 1 cut(s) 1437
BpiI GAAGAC 1 cut(s) 51
BplI GAGNNNNNCTC 2 cut(s) 294, 326
BpmI CTGGAG 1 cut(s) 486
Bpu10I CCTNAGC 1 cut(s) 1026
BsaI GGTCTC 1 cut(s) 1078
BsaJI CCNNGG 8 cut(s) 21, 205, 370, 396, 756, 930, 1034, 1388
BsaWI WCCGGW 1 cut(s) 664
BsaXI ACNNNNNCTCC 4 cut(s) 578, 608, 1006, 1036
Bsc4I CCNNNNNNNGG 4 cut(s) 932, 1257, 1258, 1442
Bse1I ACTGG 5 cut(s) 410, 503, 1021, 1115, 1139
Bse3DI GCAATG 3 cut(s) 424, 815, 876
BseBI CCWGG 5 cut(s) 1035, 1089, 1272, 1389, 1461
BseDI CCNNGG 8 cut(s) 21, 205, 370, 396, 756, 930, 1034, 1388
BseGI GGATG 2 cut(s) 333, 1030
BseLI CCNNNNNNNGG 4 cut(s) 932, 1257, 1258, 1442
BseMI GCAATG 3 cut(s) 424, 815, 876
BseMII CTCAG 4 cut(s) 231, 549, 903, 954
BseNI ACTGG 5 cut(s) 410, 503, 1021, 1115, 1139
BseXI GCAGC 5 cut(s) 58, 484, 807, 1259, 1284
Bsh1236I CGCG 3 cut(s) 398, 860, 1214
BshFI GGCC 3 cut(s) 401, 925, 1379
BsiHKAI GWGCWC 2 cut(s) 672, 1068
BsiSI CCGG 1 cut(s) 665
BslFI GGGAC 5 cut(s) 338, 662, 1033, 1128, 1405
BslI CCNNNNNNNGG 4 cut(s) 932, 1257, 1258, 1442
BsmAI GTCTC 6 cut(s) 167, 517, 580, 1078, 1102, 1389
BsmFI GGGAC 5 cut(s) 338, 662, 1033, 1128, 1405
BsnI GGCC 3 cut(s) 401, 925, 1379
Bso31I GGTCTC 1 cut(s) 1078
Bsp1286I GDGCHC 2 cut(s) 672, 1068
Bsp143I GATC 6 cut(s) 167, 361, 467, 559, 750, 1091
Bsp19I CCATGG 1 cut(s) 21
Bsp68I TCGCGA 1 cut(s) 1214
BspACI CCGC 4 cut(s) 396, 398, 775, 858
BspANI GGCC 3 cut(s) 401, 925, 1379
BspCNI CTCAG 4 cut(s) 232, 548, 904, 953
BspFNI CGCG 3 cut(s) 398, 860, 1214
BspLI GGNNCC 2 cut(s) 663, 1573
BspPI GGATC 3 cut(s) 475, 745, 1099
BspQI GCTCTTC 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 1078
BsrDI GCAATG 3 cut(s) 424, 815, 876
BsrI ACTGG 5 cut(s) 410, 503, 1021, 1115, 1139
BssECI CCNNGG 8 cut(s) 21, 205, 370, 396, 756, 930, 1034, 1388
BssMI GATC 6 cut(s) 167, 361, 467, 559, 750, 1091
BssT1I CCWWGG 5 cut(s) 21, 205, 370, 756, 930
Bst2UI CCWGG 5 cut(s) 1035, 1089, 1272, 1389, 1461
Bst4CI ACNGT 3 cut(s) 346, 746, 1174
Bst6I CTCTTC 3 cut(s) 129, 258, 595
BstAPI GCANNNNNTGC 3 cut(s) 545, 1349, 1586
BstC8I GCNNGC 2 cut(s) 923, 1582
BstDEI CTNAG 7 cut(s) 240, 535, 912, 940, 1026, 1603, 1610
BstDSI CCRYGG 2 cut(s) 21, 396
BstF5I GGATG 2 cut(s) 333, 1030
BstFNI CGCG 3 cut(s) 398, 860, 1214
BstKTI GATC 6 cut(s) 170, 364, 470, 562, 753, 1094
BstMAI GTCTC 6 cut(s) 167, 517, 580, 1078, 1102, 1389
BstMBI GATC 6 cut(s) 167, 361, 467, 559, 750, 1091
BstMWI GCNNNNNNNGC 8 cut(s) 302, 398, 545, 806, 866, 1349, 1358, 1586
BstNI CCWGG 5 cut(s) 1035, 1089, 1272, 1389, 1461
BstNSI RCATGY 3 cut(s) 229, 434, 1004
BstPAI GACNNNNGTC 1 cut(s) 1437
BstSCI CCNGG 5 cut(s) 1033, 1087, 1270, 1387, 1459
BstSFI CTRYAG 3 cut(s) 456, 687, 1241
BstUI CGCG 3 cut(s) 398, 860, 1214
BstV1I GCAGC 5 cut(s) 58, 484, 807, 1259, 1284
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 467
BstXI CCANNNNNNTGG 1 cut(s) 1568
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 3 cut(s) 401, 925, 1379
BtgI CCRYGG 2 cut(s) 21, 396
BtsCI GGATG 2 cut(s) 333, 1030
BtsI GCAGTG 1 cut(s) 1573
BtsIMutI CAGTG 3 cut(s) 510, 1014, 1573
BtuMI TCGCGA 1 cut(s) 1214
Cac8I GCNNGC 2 cut(s) 923, 1582
CaiI CAGNNNCTG 2 cut(s) 68, 1166
Cfr13I GGNCC 2 cut(s) 924, 1268
Cfr42I CCGCGG 1 cut(s) 399
CseI GACGC 1 cut(s) 537
Csp6I GTAC 3 cut(s) 210, 1050, 1497
CspCI CAANNNNNGTGG 2 cut(s) 225, 260
CviQI GTAC 3 cut(s) 210, 1050, 1497
DdeI CTNAG 7 cut(s) 240, 535, 912, 940, 1026, 1603, 1610
DpnI GATC 6 cut(s) 169, 363, 469, 561, 752, 1093
DpnII GATC 6 cut(s) 167, 361, 467, 559, 750, 1091
EaeI YGGCCR 2 cut(s) 399, 1377
Eam1104I CTCTTC 3 cut(s) 129, 258, 595
EarI CTCTTC 3 cut(s) 129, 258, 595
EciI GGCGGA 1 cut(s) 764
Ecl136II GAGCTC 1 cut(s) 1066
Eco130I CCWWGG 5 cut(s) 21, 205, 370, 756, 930
Eco24I GRGCYC 1 cut(s) 1068
Eco31I GGTCTC 1 cut(s) 1078
Eco47I GGWCC 1 cut(s) 1268
Eco53kI GAGCTC 1 cut(s) 1066
Eco57I CTGAAG 2 cut(s) 45, 492
EcoICRI GAGCTC 1 cut(s) 1066
EcoRII CCWGG 5 cut(s) 1033, 1087, 1270, 1387, 1459
EcoT14I CCWWGG 5 cut(s) 21, 205, 370, 756, 930
EcoT22I ATGCAT 1 cut(s) 1363
EcoT38I GRGCYC 1 cut(s) 1068
ErhI CCWWGG 5 cut(s) 21, 205, 370, 756, 930
FaqI GGGAC 5 cut(s) 338, 662, 1033, 1128, 1405
FauNDI CATATG 1 cut(s) 1348
FbaI TGATCA 1 cut(s) 361
FblI GTMKAC 2 cut(s) 686, 714
Fnu4HI GCNGC 7 cut(s) 72, 399, 498, 821, 858, 1248, 1298
FokI GGATG 2 cut(s) 320, 1017
FriOI GRGCYC 1 cut(s) 1068
Fsp4HI GCNGC 7 cut(s) 72, 399, 498, 821, 858, 1248, 1298
FspBI CTAG 2 cut(s) 509, 1253
GluI GCNGC 7 cut(s) 72, 399, 498, 821, 858, 1248, 1298
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 3 cut(s) 401, 925, 1379
HapII CCGG 1 cut(s) 665
HgaI GACGC 1 cut(s) 537
HinfI GANTC 7 cut(s) 17, 608, 692, 914, 938, 1021, 1336
HpaII CCGG 1 cut(s) 665
HphI GGTGA 3 cut(s) 947, 1281, 1345
Hpy166II GTNNAC 3 cut(s) 687, 715, 1268
Hpy188I TCNGA 6 cut(s) 167, 223, 467, 472, 1102, 1371
Hpy188III TCNNGA 3 cut(s) 104, 605, 1213
Hpy8I GTNNAC 3 cut(s) 687, 715, 1268
HpyAV CCTTC 5 cut(s) 7, 630, 640, 1210, 1585
HpyCH4III ACNGT 3 cut(s) 346, 746, 1174
HpyF10VI GCNNNNNNNGC 8 cut(s) 302, 398, 545, 806, 866, 1349, 1358, 1586
HpyF3I CTNAG 7 cut(s) 240, 535, 912, 940, 1026, 1603, 1610
Ksp22I TGATCA 1 cut(s) 361
KspI CCGCGG 1 cut(s) 399
Kzo9I GATC 6 cut(s) 167, 361, 467, 559, 750, 1091
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 5 cut(s) 39, 315, 372, 505, 1513
Lsp1109I GCAGC 5 cut(s) 58, 484, 807, 1259, 1284
MaeI CTAG 2 cut(s) 509, 1253
MaeIII GTNAC 5 cut(s) 340, 907, 935, 986, 1333
MalI GATC 6 cut(s) 169, 363, 469, 561, 752, 1093
MboI GATC 6 cut(s) 167, 361, 467, 559, 750, 1091
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 2 cut(s) 672, 1068
MlsI TGGCCA 1 cut(s) 1379
MluCI AATT 4 cut(s) 567, 673, 846, 1519
MluNI TGGCCA 1 cut(s) 1379
MlyI GAGTC 6 cut(s) 11, 602, 923, 932, 1015, 1330
MmeI TCCRAC 2 cut(s) 260, 445
Mox20I TGGCCA 1 cut(s) 1379
Mph1103I ATGCAT 1 cut(s) 1363
MscI TGGCCA 1 cut(s) 1379
MseI TTAA 3 cut(s) 258, 570, 1008
MslI CAYNNNNRTG 2 cut(s) 905, 1566
Msp20I TGGCCA 1 cut(s) 1379
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 1 cut(s) 665
MspR9I CCNGG 5 cut(s) 1035, 1089, 1272, 1389, 1461
MunI CAATTG 1 cut(s) 846
MvaI CCWGG 5 cut(s) 1035, 1089, 1272, 1389, 1461
MvnI CGCG 3 cut(s) 398, 860, 1214
MwoI GCNNNNNNNGC 8 cut(s) 302, 398, 545, 806, 866, 1349, 1358, 1586
NcoI CCATGG 1 cut(s) 21
NdeI CATATG 1 cut(s) 1348
NdeII GATC 6 cut(s) 167, 361, 467, 559, 750, 1091
NlaIV GGNNCC 2 cut(s) 663, 1573
NmuCI GTSAC 3 cut(s) 907, 935, 1333
NruI TCGCGA 1 cut(s) 1214
NsiI ATGCAT 1 cut(s) 1363
NspI RCATGY 3 cut(s) 229, 434, 1004
OliI CACNNNNGTG 1 cut(s) 1566
PciI ACATGT 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 258
PfeI GAWTC 1 cut(s) 692
PflFI GACNNNGTC 2 cut(s) 653, 913
PflMI CCANNNNNTGG 1 cut(s) 1442
PfoI TCCNGGA 1 cut(s) 1459
PkrI GCNGC 7 cut(s) 73, 400, 499, 822, 859, 1249, 1299
PleI GAGTC 6 cut(s) 11, 602, 922, 932, 1015, 1330
PpsI GAGTC 6 cut(s) 11, 602, 922, 932, 1015, 1330
PscI ACATGT 1 cut(s) 430
PshAI GACNNNNGTC 1 cut(s) 1437
Psp124BI GAGCTC 1 cut(s) 1068
Psp6I CCWGG 5 cut(s) 1033, 1087, 1270, 1387, 1459
PspGI CCWGG 5 cut(s) 1033, 1087, 1270, 1387, 1459
PspN4I GGNNCC 2 cut(s) 663, 1573
PspPI GGNCC 2 cut(s) 924, 1268
PstNI CAGNNNCTG 2 cut(s) 68, 1166
PsuI RGATCY 1 cut(s) 467
PsyI GACNNNGTC 2 cut(s) 653, 913
RruI TCGCGA 1 cut(s) 1214
RsaI GTAC 3 cut(s) 211, 1051, 1498
RsaNI GTAC 3 cut(s) 210, 1050, 1497
RseI CAYNNNNRTG 2 cut(s) 905, 1566
SacI GAGCTC 1 cut(s) 1068
SacII CCGCGG 1 cut(s) 399
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 3 cut(s) 258, 570, 1008
SatI GCNGC 7 cut(s) 72, 399, 498, 821, 858, 1248, 1298
Sau3AI GATC 6 cut(s) 167, 361, 467, 559, 750, 1091
Sau96I GGNCC 2 cut(s) 924, 1268
ScaI AGTACT 1 cut(s) 1051
SchI GAGTC 6 cut(s) 11, 602, 923, 932, 1015, 1330
ScrFI CCNGG 5 cut(s) 1035, 1089, 1272, 1389, 1461
SduI GDGCHC 2 cut(s) 672, 1068
SfcI CTRYAG 3 cut(s) 456, 687, 1241
Sfr303I CCGCGG 1 cut(s) 399
SgrBI CCGCGG 1 cut(s) 399
SinI GGWCC 1 cut(s) 1268
SmiMI CAYNNNNRTG 2 cut(s) 905, 1566
Sse9I AATT 4 cut(s) 567, 673, 846, 1519
SsiI CCGC 4 cut(s) 396, 398, 775, 858
SspMI CTAG 2 cut(s) 509, 1253
SstI GAGCTC 1 cut(s) 1068
StyD4I CCNGG 5 cut(s) 1033, 1087, 1270, 1387, 1459
StyI CCWWGG 5 cut(s) 21, 205, 370, 756, 930
TaaI ACNGT 3 cut(s) 346, 746, 1174
TaqI TCGA 1 cut(s) 274
TasI AATT 4 cut(s) 567, 673, 846, 1519
TatI WGTACW 2 cut(s) 1049, 1496
TauI GCSGC 2 cut(s) 401, 860
TfiI GAWTC 1 cut(s) 692
Tru1I TTAA 3 cut(s) 258, 570, 1008
Tru9I TTAA 3 cut(s) 258, 570, 1008
TscAI CASTG 3 cut(s) 510, 1021, 1573
TseFI GTSAC 3 cut(s) 907, 935, 1333
TseI GCWGC 5 cut(s) 71, 497, 820, 1247, 1297
Tsp45I GTSAC 3 cut(s) 907, 935, 1333
TspDTI ATGAA 4 cut(s) 517, 684, 691, 1332
TspRI CASTG 3 cut(s) 510, 1021, 1573
Tth111I GACNNNGTC 2 cut(s) 653, 913
Van91I CCANNNNNTGG 1 cut(s) 1442
VpaK11BI GGWCC 1 cut(s) 1268
XapI RAATTY 1 cut(s) 1519
XceI RCATGY 3 cut(s) 229, 434, 1004
XmiI GTMKAC 2 cut(s) 686, 714
XspI CTAG 2 cut(s) 509, 1253
ZrmI AGTACT 1 cut(s) 1051
Zsp2I ATGCAT 1 cut(s) 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.