Rroxscaffold_6G00427710

Chloride channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0018939
Physical Location & Seq
Reverse (-)
48268942 .. 48271827
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00427710.1

Sequence Viewer

Length: 714 bp
ATGGAAGGCCGGTGTAATGGTGGTGCGAGAAGAACCAAGCTAGGAAGAAGAAAGAGTCGGGATGATGCGATCAGGAATTTGTTTAGTGCAAAAACAATACATGAGTACAGTGCAAGTTTATTGACCTTCTTGGTTATGTTTTACACATTAGCAGTTGTGACATTTGGTACTGCTGTTCCAGCTGGTCAATTTGTTCCTGGAATAATGATAGGATCAACATATGGACGTCTTGTAGGCATGTTTGTTGTTAGCTTCTACAAGAAGCTCAACATTGAAGAGGGAACATATGCACTACTTGGAGCAGCTTCTTTTCTTGGAGGTTCAATGCGAATGACGGTGTCTCTATGTGTCATTATGGTTGAAATCACAAATAATCTGAAATTGTTGCCTCTTATCATGCTTGTTCTTCTTATTTCAAAGGCTGTTGGAGATGCATTCAACGAAGGCTTGTATGAAGAACAGTCGCGGTTAAGGAACATTCCACAGTTAGAATCAAAACCCAAATATCAAATGCGGAAGATGACAGCAAAGGAGGCTTGTGGAAAAAGGGTGATTGATCAATATTCTAGAAACGGAGAAACACTTGTCTTTGGACTAATGCTTCGTAGTCACTTGTTGGTACTTCTGCAGTCGAAGGTAGATTTCCACAAGGATGTGAAGGTAGCAAATGTACTTCTTGATGACTACCGTGAAGCCGTGGTCAGAGATTTTTGA

Protein Analysis

237

Amino Acids

26.53

Weight (kDa)

9.66

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voltage_CLC PF00654 21 - 143 8.1e-27 Voltage gated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018939)

Species Orthologous Gene IDs
pyrus_communis pycom16g15950
rosa_chinensis RchiOBHm_Chr1g0360351
rosa_multiflora Rmu_sc0003207.1_g000043 Rmu_sc0010454.1_g000010
rosa_roxburghii Rroxscaffold_3G00244910 Rroxscaffold_4G00299310 Rroxscaffold_6G00427710
rosa_samantha Rh2CG333200
rosa_wichuraiana Rw5G005100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 229
AccII CGCG 1 cut(s) 466
AciI CCGC 2 cut(s) 466, 514
AclWI GGATC 1 cut(s) 220
AcsI RAATTY 1 cut(s) 76
AcyI GRCGYC 1 cut(s) 226
AfaI GTAC 4 cut(s) 107, 169, 621, 672
AgsI TTSAA 5 cut(s) 275, 324, 362, 417, 439
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 5 cut(s) 40, 182, 252, 265, 305
AluI AGCT 5 cut(s) 40, 182, 252, 265, 305
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 1 cut(s) 220
AoxI GGCC 1 cut(s) 7
ApeKI GCWGC 1 cut(s) 302
ApoI RAATTY 1 cut(s) 76
ArsI GACNNNNNNTTYG 2 cut(s) 585, 617
AsuHPI GGTGA 1 cut(s) 562
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BbvI GCAGC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 680
BciT130I CCWGG 1 cut(s) 198
BclI TGATCA 1 cut(s) 556
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 2 cut(s) 41, 567
BfmI CTRYAG 1 cut(s) 626
BisI GCNGC 1 cut(s) 303
BlsI GCNGC 1 cut(s) 304
Bme1390I CCNGG 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 198
BmsI GCATC 2 cut(s) 55, 421
BsaHI GRCGYC 1 cut(s) 226
BsaJI CCNNGG 1 cut(s) 696
Bse118I RCCGGY 1 cut(s) 9
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 696
BseGI GGATG 2 cut(s) 67, 658
BseXI GCAGC 1 cut(s) 314
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 10
BsmAI GTCTC 1 cut(s) 345
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 1 cut(s) 9
Bsp143I GATC 3 cut(s) 69, 212, 556
BspACI CCGC 2 cut(s) 466, 514
BspANI GGCC 1 cut(s) 9
BspFNI CGCG 1 cut(s) 466
BspMAI CTGCAG 1 cut(s) 630
BspPI GGATC 1 cut(s) 220
BsrFI RCCGGY 1 cut(s) 9
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 1 cut(s) 696
BssMI GATC 3 cut(s) 69, 212, 556
BssNI GRCGYC 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 5 cut(s) 110, 337, 462, 486, 689
Bst6I CTCTTC 1 cut(s) 270
BstACI GRCGYC 1 cut(s) 226
BstDSI CCRYGG 1 cut(s) 696
BstF5I GGATG 2 cut(s) 67, 658
BstFNI CGCG 1 cut(s) 466
BstKTI GATC 3 cut(s) 72, 215, 559
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 3 cut(s) 69, 212, 556
BstMWI GCNNNNNNNGC 2 cut(s) 179, 533
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 196
BstSFI CTRYAG 1 cut(s) 626
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 1 cut(s) 314
BsuRI GGCC 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 696
BtsCI GGATG 2 cut(s) 67, 658
BtsIMutI CAGTG 1 cut(s) 115
Cfr10I RCCGGY 1 cut(s) 9
Csp6I GTAC 4 cut(s) 106, 168, 620, 671
CviAII CATG 3 cut(s) 101, 238, 397
CviQI GTAC 4 cut(s) 106, 168, 620, 671
DpnI GATC 3 cut(s) 71, 214, 558
DpnII GATC 3 cut(s) 69, 212, 556
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 196
EcoT22I ATGCAT 1 cut(s) 436
FaeI CATG 3 cut(s) 104, 241, 400
FatI CATG 3 cut(s) 100, 237, 396
FauNDI CATATG 2 cut(s) 220, 286
FbaI TGATCA 1 cut(s) 556
Fnu4HI GCNGC 1 cut(s) 303
FokI GGATG 2 cut(s) 74, 665
Fsp4HI GCNGC 1 cut(s) 303
FspBI CTAG 2 cut(s) 41, 567
GluI GCNGC 1 cut(s) 303
HaeIII GGCC 1 cut(s) 9
HapII CCGG 1 cut(s) 10
Hin1I GRCGYC 1 cut(s) 226
Hin1II CATG 3 cut(s) 104, 241, 400
HinfI GANTC 2 cut(s) 55, 491
HpaII CCGG 1 cut(s) 10
HphI GGTGA 1 cut(s) 562
Hpy188I TCNGA 2 cut(s) 378, 704
Hpy188III TCNNGA 4 cut(s) 59, 73, 567, 677
HpyAV CCTTC 4 cut(s) 136, 437, 628, 652
HpyCH4III ACNGT 5 cut(s) 110, 337, 462, 486, 689
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 5 cut(s) 89, 113, 290, 434, 628
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 533
HpySE526I ACGT 1 cut(s) 226
Hsp92I GRCGYC 1 cut(s) 226
Hsp92II CATG 3 cut(s) 104, 241, 400
Ksp22I TGATCA 1 cut(s) 556
Kzo9I GATC 3 cut(s) 69, 212, 556
LmnI GCTCC 1 cut(s) 299
LpnPI CCDG 6 cut(s) 23, 58, 168, 183, 192, 210
Lsp1109I GCAGC 1 cut(s) 314
LweI GCATC 2 cut(s) 55, 421
MaeI CTAG 2 cut(s) 41, 567
MaeII ACGT 1 cut(s) 226
MaeIII GTNAC 2 cut(s) 157, 608
MalI GATC 3 cut(s) 71, 214, 558
MboI GATC 3 cut(s) 69, 212, 556
MboII GAAGA 7 cut(s) 42, 57, 60, 287, 398, 467, 529
MluCI AATT 3 cut(s) 76, 188, 380
MlyI GAGTC 1 cut(s) 64
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 4 cut(s) 271, 311, 399, 526
Mph1103I ATGCAT 1 cut(s) 436
MseI TTAA 1 cut(s) 470
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 434
MvaI CCWGG 1 cut(s) 198
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 2 cut(s) 179, 533
NdeI CATATG 2 cut(s) 220, 286
NdeII GATC 3 cut(s) 69, 212, 556
NlaIII CATG 3 cut(s) 104, 241, 400
NmuCI GTSAC 2 cut(s) 157, 608
NsiI ATGCAT 1 cut(s) 436
NspI RCATGY 1 cut(s) 241
PctI GAATGC 1 cut(s) 434
PfeI GAWTC 1 cut(s) 491
PflFI GACNNNGTC 1 cut(s) 337
PfoI TCCNGGA 1 cut(s) 196
PkrI GCNGC 1 cut(s) 304
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PstI CTGCAG 1 cut(s) 630
PsyI GACNNNGTC 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 4 cut(s) 107, 169, 621, 672
RsaNI GTAC 4 cut(s) 106, 168, 620, 671
RseI CAYNNNNRTG 1 cut(s) 651
SaqAI TTAA 1 cut(s) 470
SatI GCNGC 1 cut(s) 303
Sau3AI GATC 3 cut(s) 69, 212, 556
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 1 cut(s) 198
SfaNI GCATC 2 cut(s) 55, 421
SfcI CTRYAG 1 cut(s) 626
SmiMI CAYNNNNRTG 1 cut(s) 651
Sse9I AATT 3 cut(s) 76, 188, 380
SsiI CCGC 2 cut(s) 466, 514
SspI AATATT 1 cut(s) 563
SspMI CTAG 2 cut(s) 41, 567
StyD4I CCNGG 1 cut(s) 196
TaaI ACNGT 5 cut(s) 110, 337, 462, 486, 689
TaiI ACGT 1 cut(s) 229
TaqI TCGA 1 cut(s) 632
TasI AATT 3 cut(s) 76, 188, 380
TatI WGTACW 2 cut(s) 105, 670
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 1 cut(s) 470
Tru9I TTAA 1 cut(s) 470
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 2 cut(s) 157, 608
TseI GCWGC 1 cut(s) 302
Tsp45I GTSAC 2 cut(s) 157, 608
TspDTI ATGAA 1 cut(s) 468
TspGWI ACGGA 1 cut(s) 588
TspRI CASTG 1 cut(s) 115
Tth111I GACNNNGTC 1 cut(s) 337
XapI RAATTY 1 cut(s) 76
XbaI TCTAGA 1 cut(s) 566
XceI RCATGY 1 cut(s) 241
XspI CTAG 2 cut(s) 41, 567
ZraI GACGTC 1 cut(s) 227
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.