Rroxscaffold_7G00170150

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007185
Physical Location & Seq
Reverse (-)
10597059 .. 10599780
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00170150.1

Sequence Viewer

Length: 1254 bp
ATGAAGCCGGAGAGGATTTTCGGCAAAATGGGGCACTTGCAGAGGCTATTGGATCGGTTTCTGTCGATCCGGCCCACCGGGTTGGCCAAGAACAACAGGATGATTCTGATTGCTTTGTACCCGGTGGTGAGGGAGAGCTTTCAGCTGTATGCCGATATTTGCGAGGTTTTGGCGGTTTTGCTTGATAAGTTTTTCGATATGGAGTATACCGATTGTGTGAAGGCGTTTGAGGCTTATGCCAGTGCGGCTAAGCAGATTGATGAGCTTGTTGCGTTCTATAGTTGGTGCAAGGACACTGGGGTTGCCAGGTCGTCGGAGTATCCAGAGATTCAGAAGATTGGGACTAAGTTATTGGAGACATTGGAGGAGTTTGTGAGGGACAGAAGTAAGAGAGTCAAGAGTCCTGAAAGGAGAGAGGAGCCTCCGGCCCCGGTCGCTCAGGAGGAGGAGCCCCAACCTGATATGAATGAGATGAAGGCTTTGCCTGCGCCTGAGAATTGGACTCCTCCACCCCCACCTGAGCCGGAGCCTCAGCCAAAGCCTCAACCAGTGGTCCAAGAAGACTTGGTGGATTTGAGAGATAATGGGGTTTCAGCTGATAATCAGGGGAATAACATGGCTTTGGCTTTGTTTGCTGGTCCTGCCGCCAATGGCAATACAAATGGTTCTTGGGAAGCGTTCCCTTCAGATGGGCAGCCCCAAGTCACCTCAGCTTGGCAGAATCCGGCTGCAGAACCCGGAAAGGCCGATTGGGAACTTGCTCTGGTCGAGACAGCAAGCAATTTAACCCACCAGAAAGCGGCAATGGGTGGTGGATTGGATCCATTGTTGTTGAATGGTATGTATGATCAGGGCATTGTGAGGCAGCATGTCGGCACTGCCCAATTGAGTGGTGGAAGTGCAAGTAGTGTTGCATTGCCCGGGCCCGGGAAGAGCGCTACACCCGTATTAGCTCTTCCCGCCCCTGACGGCACAGTGATGCCAGTGAATCAGGACCCGTTTGCTGCATCATTGACCATTCCACCTCCTTCCTATGTGCAAATGGCTGATATGGAGAAGAAGCAGCATTTACTTGTGCAGGAACAGCAGGTTTGGAATCAATATGCCAAGGAAGGAATGCAGGGCCAGACTAGCTTGGCCAAAATGAACGGGGCCGGGTTCTACGGGGCACCTCCCATGCAGATGATGCCGTATGGAATGCCTCCGGTGAATGGAATGGGGCCACCCGCCGGGTATTACTACGCTCCTTACTGA

Protein Analysis

417

Amino Acids

45.52

Weight (kDa)

4.84

Isoelectric Point (pI)

52.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 1 - 127 4.4e-36 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1078
AccB1I GGYRCC 1 cut(s) 1168
AccI GTMKAC 1 cut(s) 206
AciI CCGC 6 cut(s) 173, 245, 645, 800, 960, 1227
AclWI GGATC 4 cut(s) 60, 61, 815, 828
AcoI YGGCCR 2 cut(s) 84, 1137
AcuI CTGAAG 1 cut(s) 669
AfaI GTAC 1 cut(s) 119
AfeI AGCGCT 1 cut(s) 937
AfiI CCNNNNNNNGG 7 cut(s) 689, 714, 799, 926, 927, 1211, 1229
AgsI TTSAA 1 cut(s) 835
AjnI CCWGG 1 cut(s) 305
AjuI GAANNNNNNNTTGG 2 cut(s) 733, 765
AluBI AGCT 7 cut(s) 138, 145, 265, 596, 713, 953, 1134
AluI AGCT 7 cut(s) 138, 145, 265, 596, 713, 953, 1134
Alw26I GTCTC 2 cut(s) 350, 764
AlwI GGATC 4 cut(s) 60, 61, 815, 828
Ama87I CYCGRG 2 cut(s) 920, 926
Aor51HI AGCGCT 1 cut(s) 937
AoxI GGCC 9 cut(s) 71, 84, 426, 744, 923, 1123, 1137, 1152, 1220
ApaI GGGCCC 1 cut(s) 927
ApeKI GCWGC 5 cut(s) 694, 728, 865, 1004, 1063
AspLEI GCGC 2 cut(s) 490, 938
AsuHPI GGTGA 3 cut(s) 139, 697, 1219
AvaI CYCGRG 2 cut(s) 920, 926
AvaII GGWCC 3 cut(s) 553, 638, 994
BaeGI GKGCMC 3 cut(s) 36, 927, 1171
BalI TGGCCA 2 cut(s) 86, 1139
BamHI GGATCC 1 cut(s) 820
BanI GGYRCC 1 cut(s) 1168
BanII GRGCYC 2 cut(s) 453, 927
BarI GAAGNNNNNNTAC 2 cut(s) 939, 971
BbsI GAAGAC 1 cut(s) 567
BbvCI CCTCAGC 2 cut(s) 531, 709
BbvI GCAGC 5 cut(s) 706, 715, 877, 991, 1075
BccI CCATC 1 cut(s) 683
BceAI ACGGC 2 cut(s) 985, 1174
BciT130I CCWGG 1 cut(s) 307
BciVI GTATCC 1 cut(s) 330
BclI TGATCA 1 cut(s) 847
BcoDI GTCTC 2 cut(s) 350, 764
BfaI CTAG 1 cut(s) 1131
BfmI CTRYAG 2 cut(s) 277, 729
BfoI RGCGCY 1 cut(s) 939
BfuAI ACCTGC 1 cut(s) 1078
BfuI GTATCC 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 245
BisI GCNGC 8 cut(s) 246, 645, 695, 729, 801, 866, 1005, 1064
BlpI GCTNAGC 1 cut(s) 249
BlsI GCNGC 8 cut(s) 247, 646, 696, 730, 802, 867, 1006, 1065
Bme18I GGWCC 3 cut(s) 553, 638, 994
BmeT110I CYCGRG 2 cut(s) 920, 926
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 3 cut(s) 969, 1016, 1176
BmuI ACTGGG 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 567
Bpu10I CCTNAGC 4 cut(s) 438, 519, 531, 709
Bpu1102I GCTNAGC 1 cut(s) 249
BsaJI CCNNGG 4 cut(s) 429, 920, 926, 1107
BsaWI WCCGGW 1 cut(s) 1204
Bsc4I CCNNNNNNNGG 7 cut(s) 689, 714, 799, 926, 927, 1211, 1229
Bse1I ACTGG 4 cut(s) 240, 301, 548, 983
Bse3DI GCAATG 2 cut(s) 810, 914
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 4 cut(s) 429, 920, 926, 1107
BseGI GGATG 1 cut(s) 105
BseLI CCNNNNNNNGG 7 cut(s) 689, 714, 799, 926, 927, 1211, 1229
BseMI GCAATG 2 cut(s) 810, 914
BseMII CTCAG 5 cut(s) 452, 483, 510, 545, 723
BseNI ACTGG 4 cut(s) 240, 301, 548, 983
BseRI GAGGAG 5 cut(s) 380, 431, 458, 461, 495
BseSI GKGCMC 3 cut(s) 36, 927, 1171
BseXI GCAGC 5 cut(s) 706, 715, 877, 991, 1075
BsgI GTGCAG 1 cut(s) 1097
Bsh1285I CGRYCG 1 cut(s) 435
BshFI GGCC 9 cut(s) 73, 86, 428, 746, 925, 1125, 1139, 1154, 1222
BshNI GGYRCC 1 cut(s) 1168
BsiEI CGRYCG 1 cut(s) 435
BsiHKCI CYCGRG 2 cut(s) 920, 926
BslFI GGGAC 2 cut(s) 355, 392
BslI CCNNNNNNNGG 7 cut(s) 689, 714, 799, 926, 927, 1211, 1229
BsmAI GTCTC 2 cut(s) 350, 764
BsmFI GGGAC 2 cut(s) 355, 392
BsmI GAATGC 2 cut(s) 1122, 1203
BsnI GGCC 9 cut(s) 73, 86, 428, 746, 925, 1125, 1139, 1154, 1222
BsoBI CYCGRG 2 cut(s) 920, 926
Bsp120I GGGCCC 1 cut(s) 923
Bsp1286I GDGCHC 4 cut(s) 36, 453, 927, 1171
Bsp143I GATC 4 cut(s) 52, 66, 820, 847
Bsp1720I GCTNAGC 1 cut(s) 249
BspACI CCGC 6 cut(s) 173, 245, 645, 800, 960, 1227
BspANI GGCC 9 cut(s) 73, 86, 428, 746, 925, 1125, 1139, 1154, 1222
BspCNI CTCAG 5 cut(s) 451, 484, 511, 544, 722
BspMAI CTGCAG 1 cut(s) 733
BspMI ACCTGC 1 cut(s) 1078
BspPI GGATC 4 cut(s) 60, 61, 815, 828
BspQI GCTCTTC 2 cut(s) 926, 960
BspT107I GGYRCC 1 cut(s) 1168
BsrDI GCAATG 2 cut(s) 810, 914
BsrI ACTGG 4 cut(s) 240, 301, 548, 983
BssECI CCNNGG 4 cut(s) 429, 920, 926, 1107
BssMI GATC 4 cut(s) 52, 66, 820, 847
BssNAI GTATAC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 1107
Bst1107I GTATAC 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 307
Bst4CI ACNGT 1 cut(s) 976
Bst6I CTCTTC 2 cut(s) 926, 960
BstAPI GCANNNNNTGC 1 cut(s) 1186
BstC8I GCNNGC 2 cut(s) 486, 778
BstDEI CTNAG 7 cut(s) 249, 345, 438, 492, 519, 531, 709
BstF5I GGATG 1 cut(s) 105
BstH2I RGCGCY 1 cut(s) 939
BstHHI GCGC 2 cut(s) 490, 938
BstKTI GATC 4 cut(s) 55, 69, 823, 850
BstMAI GTCTC 2 cut(s) 350, 764
BstMBI GATC 4 cut(s) 52, 66, 820, 847
BstMCI CGRYCG 1 cut(s) 435
BstNI CCWGG 1 cut(s) 307
BstNSI RCATGY 1 cut(s) 872
BstSFI CTRYAG 2 cut(s) 277, 729
BstSLI GKGCMC 3 cut(s) 36, 927, 1171
BstV1I GCAGC 5 cut(s) 706, 715, 877, 991, 1075
BstV2I GAAGAC 1 cut(s) 567
BstX2I RGATCY 1 cut(s) 820
BstXI CCANNNNNNTGG 2 cut(s) 82, 890
BstYI RGATCY 1 cut(s) 820
BstZ17I GTATAC 1 cut(s) 207
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 9 cut(s) 73, 86, 428, 746, 925, 1125, 1139, 1154, 1222
BtsCI GGATG 1 cut(s) 105
BtsI GCAGTG 1 cut(s) 876
BtsIMutI CAGTG 6 cut(s) 247, 294, 555, 876, 981, 990
BveI ACCTGC 1 cut(s) 1078
Cac8I GCNNGC 2 cut(s) 486, 778
CfoI GCGC 2 cut(s) 490, 938
Cfr9I CCCGGG 2 cut(s) 920, 926
Csp6I GTAC 1 cut(s) 118
CviAII CATG 3 cut(s) 616, 869, 1177
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 7 cut(s) 249, 345, 438, 492, 519, 531, 709
DpnI GATC 4 cut(s) 54, 68, 822, 849
DpnII GATC 4 cut(s) 52, 66, 820, 847
EaeI YGGCCR 2 cut(s) 84, 1137
Eam1104I CTCTTC 2 cut(s) 926, 960
EarI CTCTTC 2 cut(s) 926, 960
Eco130I CCWWGG 1 cut(s) 1107
Eco24I GRGCYC 2 cut(s) 453, 927
Eco47I GGWCC 3 cut(s) 553, 638, 994
Eco47III AGCGCT 1 cut(s) 937
Eco57I CTGAAG 1 cut(s) 669
Eco88I CYCGRG 2 cut(s) 920, 926
EcoO109I RGGNCCY 1 cut(s) 994
EcoRII CCWGG 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 1107
EcoT38I GRGCYC 2 cut(s) 453, 927
ErhI CCWWGG 1 cut(s) 1107
FaeI CATG 3 cut(s) 619, 872, 1180
FaqI GGGAC 2 cut(s) 355, 392
FatI CATG 3 cut(s) 615, 868, 1176
FauI CCCGC 2 cut(s) 967, 1234
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 8 cut(s) 246, 645, 695, 729, 801, 866, 1005, 1064
FokI GGATG 1 cut(s) 112
FriOI GRGCYC 2 cut(s) 453, 927
Fsp4HI GCNGC 8 cut(s) 246, 645, 695, 729, 801, 866, 1005, 1064
FspBI CTAG 1 cut(s) 1131
GlaI GCGC 2 cut(s) 489, 937
GluI GCNGC 8 cut(s) 246, 645, 695, 729, 801, 866, 1005, 1064
HaeII RGCGCY 1 cut(s) 939
HaeIII GGCC 9 cut(s) 73, 86, 428, 746, 925, 1125, 1139, 1154, 1222
HhaI GCGC 2 cut(s) 490, 938
Hin1II CATG 3 cut(s) 619, 872, 1180
Hin6I GCGC 2 cut(s) 488, 936
HinP1I GCGC 2 cut(s) 488, 936
HinfI GANTC 8 cut(s) 103, 328, 393, 400, 502, 721, 988, 1096
HphI GGTGA 3 cut(s) 139, 697, 1219
Hpy166II GTNNAC 1 cut(s) 207
Hpy188I TCNGA 4 cut(s) 108, 316, 333, 688
Hpy188III TCNNGA 6 cut(s) 323, 397, 404, 440, 769, 992
Hpy8I GTNNAC 1 cut(s) 207
Hpy99I CGWCG 1 cut(s) 316
HpyAV CCTTC 5 cut(s) 214, 469, 693, 1038, 1106
HpyCH4III ACNGT 1 cut(s) 976
HpyF3I CTNAG 7 cut(s) 249, 345, 438, 492, 519, 531, 709
Hsp92II CATG 3 cut(s) 619, 872, 1180
HspAI GCGC 2 cut(s) 488, 936
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 4 cut(s) 52, 66, 820, 847
LguI GCTCTTC 2 cut(s) 926, 960
LmnI GCTCC 4 cut(s) 418, 448, 526, 1249
Lsp1109I GCAGC 5 cut(s) 706, 715, 877, 991, 1075
LweI GCATC 3 cut(s) 969, 1016, 1176
MaeI CTAG 1 cut(s) 1131
MaeIII GTNAC 1 cut(s) 703
MalI GATC 4 cut(s) 54, 68, 822, 849
MboI GATC 4 cut(s) 52, 66, 820, 847
MboII GAAGA 5 cut(s) 346, 572, 943, 947, 1069
MfeI CAATTG 1 cut(s) 884
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 4 cut(s) 36, 453, 927, 1171
MlsI TGGCCA 2 cut(s) 86, 1139
MluCI AATT 3 cut(s) 496, 781, 884
MluNI TGGCCA 2 cut(s) 86, 1139
MlyI GAGTC 3 cut(s) 402, 409, 496
MmeI TCCRAC 1 cut(s) 294
Mox20I TGGCCA 2 cut(s) 86, 1139
MscI TGGCCA 2 cut(s) 86, 1139
MseI TTAA 1 cut(s) 785
MslI CAYNNNNRTG 2 cut(s) 977, 1181
Msp20I TGGCCA 2 cut(s) 86, 1139
MspA1I CMGCKG 2 cut(s) 145, 596
MunI CAATTG 1 cut(s) 884
Mva1269I GAATGC 2 cut(s) 1122, 1203
MvaI CCWGG 1 cut(s) 307
NdeII GATC 4 cut(s) 52, 66, 820, 847
NlaIII CATG 3 cut(s) 619, 872, 1180
NmuCI GTSAC 1 cut(s) 703
NspI RCATGY 1 cut(s) 872
PciSI GCTCTTC 2 cut(s) 926, 960
PctI GAATGC 2 cut(s) 1122, 1203
PfeI GAWTC 5 cut(s) 103, 328, 721, 988, 1096
PkrI GCNGC 8 cut(s) 247, 646, 696, 730, 802, 867, 1006, 1065
PleI GAGTC 3 cut(s) 401, 408, 496
PpsI GAGTC 3 cut(s) 401, 408, 496
PpuMI RGGWCCY 1 cut(s) 994
Psp5II RGGWCCY 1 cut(s) 994
Psp6I CCWGG 1 cut(s) 305
PspGI CCWGG 1 cut(s) 305
PspOMI GGGCCC 1 cut(s) 923
PspPPI RGGWCCY 1 cut(s) 994
PstI CTGCAG 1 cut(s) 733
PsuI RGATCY 1 cut(s) 820
PvuII CAGCTG 2 cut(s) 145, 596
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
RseI CAYNNNNRTG 2 cut(s) 977, 1181
SapI GCTCTTC 2 cut(s) 926, 960
SaqAI TTAA 1 cut(s) 785
SatI GCNGC 8 cut(s) 246, 645, 695, 729, 801, 866, 1005, 1064
Sau3AI GATC 4 cut(s) 52, 66, 820, 847
SchI GAGTC 3 cut(s) 402, 409, 496
SduI GDGCHC 4 cut(s) 36, 453, 927, 1171
SfaNI GCATC 3 cut(s) 969, 1016, 1176
SfcI CTRYAG 2 cut(s) 277, 729
SinI GGWCC 3 cut(s) 553, 638, 994
SmaI CCCGGG 2 cut(s) 922, 928
SmiMI CAYNNNNRTG 2 cut(s) 977, 1181
SrfI GCCCGGGC 1 cut(s) 922
Sse9I AATT 3 cut(s) 496, 781, 884
SsiI CCGC 6 cut(s) 173, 245, 645, 800, 960, 1227
SspMI CTAG 1 cut(s) 1131
StyI CCWWGG 1 cut(s) 1107
TaaI ACNGT 1 cut(s) 976
TaqI TCGA 3 cut(s) 65, 195, 768
TasI AATT 3 cut(s) 496, 781, 884
TauI GCSGC 3 cut(s) 248, 647, 803
TfiI GAWTC 5 cut(s) 103, 328, 721, 988, 1096
Tru1I TTAA 1 cut(s) 785
Tru9I TTAA 1 cut(s) 785
TscAI CASTG 6 cut(s) 247, 301, 555, 883, 981, 990
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 5 cut(s) 694, 728, 865, 1004, 1063
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 4 cut(s) 17, 479, 488, 1160
TspMI CCCGGG 2 cut(s) 920, 926
TspRI CASTG 6 cut(s) 247, 301, 555, 883, 981, 990
VpaK11BI GGWCC 3 cut(s) 553, 638, 994
XceI RCATGY 1 cut(s) 872
XcmI CCANNNNNNNNNTGG 1 cut(s) 890
XmaI CCCGGG 2 cut(s) 920, 926
XmiI GTMKAC 1 cut(s) 206
XspI CTAG 1 cut(s) 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.