Rorug01G0000800

Thioredoxin-like protein Clot

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0013216
Physical Location & Seq
Forward (+)
280687 .. 283198
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0000800.1

Sequence Viewer

Length: 1188 bp
ATGTCGATCCAAGTTGGTCCAACTGTTGAGTTACAAAACACCATGCCCGAAATTGCTCGTCCAATGACAAGTTTTCAACCAGGCATTTCCATAGATGAGTTCATCGACTGTCACTCCCTCGACAGCATAACTCATGCCCGTATGCAACAGCAAATTGACGAACTGAAAGTATTAGTGAAGGGAGAAGTTTTCGCATCATATGATGTTTCTTATCAGCTGAAATTAATTGATGAAATCCAACGCCTAGGCGTGGCATACCATTTTGAAACAGAAATAGATGAAGCACTAGAAAATATCTATGTTAAATACCATGATGATGGTGATGTCGACCTATACAATGTTTCTCTTCGTTTTCGTCTGCTAAGACAACATGGATATAATGTTTCATCTGATATATTCAGCAAATTTAAAGATGCAAACGGTTACTTCAAGGAAAGCTTAATTGTTGATATCTTGGCTATCCTATGCTTGTATGAGGCAACACATCTTAGGGTTCATGGAGAAGAAATACTAGAAGAAGCTCTTGTTTTCACCACCACCCACCTTGAATCAGCGATAAGTGGTGTAAGCTATCCACTAGCTGCAAAAATATCTCAAGCCCTAGAGAGGCCTCTGCGTAGAGGTGTAGAGAGGTTATGTGCCAGGAATTACATACCAATCTACCAGGCCACGACTCCGCATAACGAAACCTTACTGAAACTTGCGAAGTTAGATTTCAATCTGGTTCAATCTTTACACAAAGAGGAGCTTAGTGAGCTTTCAAGGTGGGCGAAAGAACTAGGCTTTGAAAAGAATCTTCCTAGCGTAAGGCTTAGGATTGTGGAGGCTTTCTTGTGGATGGTGGGAATGTATTTTGAACCCCGATACTCGGTTGGAAGAATAATCGGAACAAAACTGGGTTTTCTAGCTATAATATTGGATGATATCTATGATGCATGTGGTACATTCGAAGAACTCAAGATACTTAGTGAAGCAATTGACAGGTTTGATGTCAACTATTGCATGAATGGTCTACCACGATACATGCAAGTATTCTATCATTCACTTTTGAAGACTATGAATGAAGTTGAGGAAGAGCTAGAAAAGCAAGGAATATCGTACCGAGTCCACTACGCAAAACAAGTTGCAAATTTAGTAGAAATGATGAATAAACTTGGTAGTAATGTTACAAAGAAACCTGTTACATAG

Protein Analysis

395

Amino Acids

45.41

Weight (kDa)

5.33

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 42 - 201 5.2e-53 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 258 - 383 4.4e-38 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 300 - 380 2.9e-09 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013216)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42850 AT5G42850
fragaria_vesca FvH4_7g00510
malus_domestica MD07G1004900.v1.1
prunus_persica Prupe.2G007200_v2.0.a1 Prupe.2G007200_v2.0.a1
pyrus_communis pycom07g00400
rosa_chinensis RchiOBHm_Chr1g0314301
rosa_laevigata RLG00000030768
rosa_multiflora Rmu_sc0000343.1_g000014 Rmu_sc0002833.1_g000004
rosa_roxburghii Rroxscaffold_4G00331980
rosa_rugosa Rorug01G0000800
rosa_samantha Rh1AG009900 Rh1BG002800 Rh1CG010200 Rh1DG005300
rosa_wichuraiana Rw1G000440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 327, 1012
AciI CCGC 1 cut(s) 677
AcsI RAATTY 2 cut(s) 404, 1129
AfaI GTAC 2 cut(s) 943, 1100
AfiI CCNNNNNNNGG 3 cut(s) 250, 606, 868
AjnI CCWGG 3 cut(s) 79, 641, 663
AluBI AGCT 9 cut(s) 217, 438, 521, 570, 581, 748, 757, 908, 1078
AluI AGCT 9 cut(s) 217, 438, 521, 570, 581, 748, 757, 908, 1078
AoxI GGCC 2 cut(s) 608, 666
ApeKI GCWGC 1 cut(s) 581
ApoI RAATTY 2 cut(s) 404, 1129
AseI ATTAAT 1 cut(s) 224
AspA2I CCTAGG 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 2 cut(s) 332, 523
AsuII TTCGAA 1 cut(s) 948
AvaII GGWCC 1 cut(s) 17
AvrII CCTAGG 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 1058
BbvI GCAGC 1 cut(s) 568
BccI CCATC 2 cut(s) 311, 832
BciT130I CCWGG 3 cut(s) 81, 643, 665
BfaI CTAG 9 cut(s) 245, 287, 512, 578, 602, 779, 801, 905, 1079
BisI GCNGC 1 cut(s) 582
BlnI CCTAGG 1 cut(s) 244
BlsI GCNGC 1 cut(s) 583
Bme1390I CCNGG 3 cut(s) 81, 643, 665
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmrFI CCNGG 3 cut(s) 81, 643, 665
BmrI ACTGGG 1 cut(s) 905
BmsI GCATC 3 cut(s) 203, 403, 922
BmuI ACTGGG 1 cut(s) 905
BpiI GAAGAC 1 cut(s) 1058
Bpu10I CCTNAGC 1 cut(s) 812
Bpu14I TTCGAA 1 cut(s) 948
BpuEI CTTGAG 2 cut(s) 579, 941
BsaBI GATNNNNATC 1 cut(s) 717
BsaJI CCNNGG 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 3 cut(s) 250, 606, 868
Bse1I ACTGG 1 cut(s) 900
Bse8I GATNNNNATC 1 cut(s) 717
BseBI CCWGG 3 cut(s) 81, 643, 665
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 2 cut(s) 843, 925
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 3 cut(s) 250, 606, 868
BseNI ACTGG 1 cut(s) 900
BseRI GAGGAG 1 cut(s) 758
BseXI GCAGC 1 cut(s) 568
BshFI GGCC 2 cut(s) 610, 668
BslI CCNNNNNNNGG 3 cut(s) 250, 606, 868
BsnI GGCC 2 cut(s) 610, 668
Bsp119I TTCGAA 1 cut(s) 948
Bsp143I GATC 1 cut(s) 6
BspACI CCGC 1 cut(s) 677
BspANI GGCC 2 cut(s) 610, 668
BspQI GCTCTTC 1 cut(s) 1068
BspT104I TTCGAA 1 cut(s) 948
BsrI ACTGG 1 cut(s) 900
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 3 cut(s) 81, 643, 665
Bst4CI ACNGT 3 cut(s) 25, 110, 422
Bst6I CTCTTC 2 cut(s) 351, 1068
BstBI TTCGAA 1 cut(s) 948
BstDEI CTNAG 5 cut(s) 362, 488, 749, 812, 965
BstF5I GGATG 2 cut(s) 843, 925
BstKTI GATC 1 cut(s) 9
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 2 cut(s) 754, 1084
BstNI CCWGG 3 cut(s) 81, 643, 665
BstNSI RCATGY 2 cut(s) 939, 1027
BstSCI CCNGG 3 cut(s) 79, 641, 663
BstV1I GCAGC 1 cut(s) 568
BstV2I GAAGAC 1 cut(s) 1058
BstXI CCANNNNNNTGG 1 cut(s) 317
BsuRI GGCC 2 cut(s) 610, 668
BtsCI GGATG 2 cut(s) 843, 925
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 2 cut(s) 942, 1099
CspCI CAANNNNNGTGG 2 cut(s) 526, 561
CviAII CATG 8 cut(s) 43, 134, 311, 371, 497, 936, 1003, 1024
CviQI GTAC 2 cut(s) 942, 1099
DdeI CTNAG 5 cut(s) 362, 488, 749, 812, 965
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
DraI TTTAAA 1 cut(s) 409
Eam1104I CTCTTC 2 cut(s) 351, 1068
EarI CTCTTC 2 cut(s) 351, 1068
Eco130I CCWWGG 1 cut(s) 244
Eco147I AGGCCT 1 cut(s) 610
Eco32I GATATC 2 cut(s) 451, 925
Eco47I GGWCC 1 cut(s) 17
EcoRII CCWGG 3 cut(s) 79, 641, 663
EcoRV GATATC 2 cut(s) 451, 925
EcoT14I CCWWGG 1 cut(s) 244
EcoT22I ATGCAT 1 cut(s) 937
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 8 cut(s) 46, 137, 314, 374, 500, 939, 1006, 1027
FalI AAGNNNNNCTT 6 cut(s) 422, 454, 507, 539, 732, 764
FatI CATG 8 cut(s) 42, 133, 310, 370, 496, 935, 1002, 1023
FauNDI CATATG 1 cut(s) 199
FblI GTMKAC 2 cut(s) 327, 1012
Fnu4HI GCNGC 1 cut(s) 582
FokI GGATG 2 cut(s) 850, 932
Fsp4HI GCNGC 1 cut(s) 582
FspBI CTAG 9 cut(s) 245, 287, 512, 578, 602, 779, 801, 905, 1079
GluI GCNGC 1 cut(s) 582
HaeIII GGCC 2 cut(s) 610, 668
Hin1II CATG 8 cut(s) 46, 137, 314, 374, 500, 939, 1006, 1027
HincII GTYRAC 2 cut(s) 328, 994
HindII GTYRAC 2 cut(s) 328, 994
HindIII AAGCTT 1 cut(s) 436
HinfI GANTC 4 cut(s) 548, 673, 793, 1104
HphI GGTGA 2 cut(s) 332, 523
Hpy166II GTNNAC 4 cut(s) 328, 994, 1013, 1108
Hpy188I TCNGA 2 cut(s) 391, 887
Hpy188III TCNNGA 1 cut(s) 958
Hpy8I GTNNAC 4 cut(s) 328, 994, 1013, 1108
HpyAV CCTTC 1 cut(s) 172
HpyCH4III ACNGT 3 cut(s) 25, 110, 422
HpyCH4V TGCA 7 cut(s) 145, 416, 584, 935, 1002, 1027, 1127
HpyF10VI GCNNNNNNNGC 2 cut(s) 754, 1084
HpyF3I CTNAG 5 cut(s) 362, 488, 749, 812, 965
Hsp92II CATG 8 cut(s) 46, 137, 314, 374, 500, 939, 1006, 1027
Kzo9I GATC 1 cut(s) 6
LguI GCTCTTC 1 cut(s) 1068
LmnI GCTCC 1 cut(s) 745
LpnPI CCDG 9 cut(s) 66, 93, 628, 650, 655, 677, 707, 881, 967
Lsp1109I GCAGC 1 cut(s) 568
LweI GCATC 3 cut(s) 203, 403, 922
MaeI CTAG 9 cut(s) 245, 287, 512, 578, 602, 779, 801, 905, 1079
MaeIII GTNAC 5 cut(s) 30, 110, 422, 1165, 1180
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 8 cut(s) 338, 515, 527, 788, 888, 962, 1063, 1085
MfeI CAATTG 1 cut(s) 975
MluCI AATT 9 cut(s) 51, 153, 221, 225, 404, 441, 646, 975, 1129
MlyI GAGTC 2 cut(s) 667, 1113
MmeI TCCRAC 3 cut(s) 44, 262, 853
MnlI CCTC 9 cut(s) 128, 469, 600, 614, 621, 624, 736, 817, 1063
Mph1103I ATGCAT 1 cut(s) 937
MseI TTAA 4 cut(s) 224, 303, 408, 440
MslI CAYNNNNRTG 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 217
MspR9I CCNGG 3 cut(s) 81, 643, 665
MunI CAATTG 1 cut(s) 975
MvaI CCWGG 3 cut(s) 81, 643, 665
MwoI GCNNNNNNNGC 2 cut(s) 754, 1084
NdeI CATATG 1 cut(s) 199
NdeII GATC 1 cut(s) 6
NlaIII CATG 8 cut(s) 46, 137, 314, 374, 500, 939, 1006, 1027
NmuCI GTSAC 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 937
NspI RCATGY 2 cut(s) 939, 1027
NspV TTCGAA 1 cut(s) 948
PceI AGGCCT 1 cut(s) 610
PciSI GCTCTTC 1 cut(s) 1068
PfeI GAWTC 2 cut(s) 548, 793
PkrI GCNGC 1 cut(s) 583
PleI GAGTC 2 cut(s) 667, 1112
PpsI GAGTC 2 cut(s) 667, 1112
PshBI ATTAAT 1 cut(s) 224
Psp6I CCWGG 3 cut(s) 79, 641, 663
PspGI CCWGG 3 cut(s) 79, 641, 663
PspPI GGNCC 1 cut(s) 17
PsrI GAACNNNNNNTAC 2 cut(s) 945, 977
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 2 cut(s) 943, 1100
RsaNI GTAC 2 cut(s) 942, 1099
RseI CAYNNNNRTG 1 cut(s) 315
SalI GTCGAC 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 1068
SaqAI TTAA 4 cut(s) 224, 303, 408, 440
SatI GCNGC 1 cut(s) 582
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 17
SchI GAGTC 2 cut(s) 667, 1113
ScrFI CCNGG 3 cut(s) 81, 643, 665
SfaNI GCATC 3 cut(s) 203, 403, 922
SfuI TTCGAA 1 cut(s) 948
SinI GGWCC 1 cut(s) 17
SmiMI CAYNNNNRTG 1 cut(s) 315
SmlI CTYRAG 2 cut(s) 594, 956
SmoI CTYRAG 2 cut(s) 594, 956
Sse9I AATT 9 cut(s) 51, 153, 221, 225, 404, 441, 646, 975, 1129
SseBI AGGCCT 1 cut(s) 610
SsiI CCGC 1 cut(s) 677
SspI AATATT 1 cut(s) 915
SspMI CTAG 9 cut(s) 245, 287, 512, 578, 602, 779, 801, 905, 1079
StuI AGGCCT 1 cut(s) 610
StyD4I CCNGG 3 cut(s) 79, 641, 663
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 3 cut(s) 25, 110, 422
TaqI TCGA 5 cut(s) 5, 105, 120, 327, 948
TasI AATT 9 cut(s) 51, 153, 221, 225, 404, 441, 646, 975, 1129
TfiI GAWTC 2 cut(s) 548, 793
Tru1I TTAA 4 cut(s) 224, 303, 408, 440
Tru9I TTAA 4 cut(s) 224, 303, 408, 440
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 581
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 9 cut(s) 91, 246, 294, 375, 485, 1019, 1073, 1077, 1160
VpaK11BI GGWCC 1 cut(s) 17
VspI ATTAAT 1 cut(s) 224
XapI RAATTY 2 cut(s) 404, 1129
XceI RCATGY 2 cut(s) 939, 1027
XmaJI CCTAGG 1 cut(s) 244
XmiI GTMKAC 2 cut(s) 327, 1012
XspI CTAG 9 cut(s) 245, 287, 512, 578, 602, 779, 801, 905, 1079
Zsp2I ATGCAT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.