Rorug01G0168600

glycine--tRNA ligase 1, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0019946
Physical Location & Seq
Forward (+)
22994951 .. 23000570
5620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0168600.1

Sequence Viewer

Length: 888 bp
ATGGGGTGCAACCTGGATTTATTTGGCCACGGGGATGACTATTACCTCAAACTAGCAACAAATCCTTCCCTTGCCAAGCATGCTGGTGCCTATAAGGGTGGCCCTGACTATGGGGTATTCAGTTGGGATAACAAACTTGCTGGTGCTCAGGTGCTGCTGACCCGCATAAGGTTATTCTTGAAACCTGGTTACCCATATGAAGAAATTCTGTGGACATATCATAATCAGACTACCACTTTCATGTGCTCATACCTGCCAGTTTTCACATACTTTAACCGAACCAAAGCCTTTTTAGCCACCCTGTTTAGCGATTACCTTAATGCTGCTGCTGGTACACATGGTTGGCATTGTGGTGCCAACTACTTATCTAGTAATGTGTTGCGTGAATTTGCCAAGTTTCAGATTGATTACATTCTCGGCAACAATCCCCAGAAAATGAGCTATGTTGTGGGTTTCGGCGATCAATATCCCCTTCATGTCCACCATAGGGCAGCATCTATTCCTTATAATAAGGTTAGATATGATTGTGAAGGAGGATGGCAGTGGAGGGACTCGTCAAAGCCCAACTCCAACATCATTGAGGGAGCAATGGTTGGAGGCCCTGACATTGATGATGTATTTGAAGATTTGAGGAACAATTCCAGCCAGACAGAGCCGACTATTGCTGGAAATGCTGGTTTAGTAGCTGCATTGGTGGCTCTGTCAGCGAACAATCAGCCAACATCAGCAAAACCGGCATCTTCTCCCAGGTGTTTCCCCCTCCACCACGCTAAACAGGTGTTTCATTTTTTTTTTTTTTTTTTTTTAAAAAGTGTAAAGAGATACAAAATGGGTGATGTAGCACCAAAGCCTTCAACTTCGAATCTTTGTTCTTTTCTTCTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
HGTP_anticodon WHEP-TRS tRNA-synt_2b

Protein Analysis

295

Amino Acids

33.0

Weight (kDa)

8.61

Isoelectric Point (pI)

34.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 12 - 231 2.2e-47 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019946)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0024621
rosa_rugosa Rorug01G0168600
rosa_samantha Rh1DG125500 Rh2CG453400 Rh4AG099400 Rh4CG109000 Rh6DG197700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 507
Acc36I ACCTGC 1 cut(s) 261
AccB1I GGYRCC 2 cut(s) 86, 353
AciI CCGC 1 cut(s) 163
AcoI YGGCCR 1 cut(s) 25
AcsI RAATTY 2 cut(s) 204, 386
AfaI GTAC 1 cut(s) 334
AfiI CCNNNNNNNGG 3 cut(s) 110, 168, 487
AgsI TTSAA 3 cut(s) 181, 623, 855
AjnI CCWGG 3 cut(s) 12, 184, 746
AluBI AGCT 2 cut(s) 441, 686
AluI AGCT 2 cut(s) 441, 686
Alw21I GWGCWC 2 cut(s) 148, 248
AlwNI CAGNNNCTG 1 cut(s) 154
AoxI GGCC 3 cut(s) 25, 100, 598
ApeKI GCWGC 5 cut(s) 154, 323, 326, 491, 686
ApoI RAATTY 2 cut(s) 204, 386
Asp700I GAANNNNTTC 1 cut(s) 204
AspS9I GGNCC 2 cut(s) 101, 599
AsuHPI GGTGA 1 cut(s) 845
AsuII TTCGAA 1 cut(s) 860
BalI TGGCCA 1 cut(s) 27
BanI GGYRCC 2 cut(s) 86, 353
Bbv12I GWGCWC 2 cut(s) 148, 248
BbvI GCAGC 5 cut(s) 141, 310, 313, 503, 673
BccI CCATC 1 cut(s) 531
BciT130I CCWGG 3 cut(s) 14, 186, 748
BfaI CTAG 3 cut(s) 53, 369, 886
BfuAI ACCTGC 1 cut(s) 261
BisI GCNGC 5 cut(s) 155, 324, 327, 492, 687
BlsI GCNGC 5 cut(s) 156, 325, 328, 493, 688
Bme1390I CCNGG 3 cut(s) 14, 186, 748
BmgT120I GGNCC 2 cut(s) 101, 599
BmiI GGNNCC 2 cut(s) 88, 355
BmrFI CCNGG 3 cut(s) 14, 186, 748
BmsI GCATC 2 cut(s) 503, 746
Bpu10I CCTNAGC 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 860
BsaBI GATNNNNATC 1 cut(s) 465
BsaJI CCNNGG 2 cut(s) 28, 746
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 168, 487
Bse118I RCCGGY 1 cut(s) 733
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 465
BseBI CCWGG 3 cut(s) 14, 186, 748
BseDI CCNNGG 2 cut(s) 28, 746
BseGI GGATG 2 cut(s) 40, 542
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 3 cut(s) 110, 168, 487
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 1 cut(s) 257
BseXI GCAGC 5 cut(s) 141, 310, 313, 503, 673
BshFI GGCC 3 cut(s) 27, 102, 600
BshNI GGYRCC 2 cut(s) 86, 353
BsiHKAI GWGCWC 2 cut(s) 148, 248
BsiSI CCGG 1 cut(s) 734
BslFI GGGAC 1 cut(s) 563
BslI CCNNNNNNNGG 3 cut(s) 110, 168, 487
BsmFI GGGAC 1 cut(s) 563
BsnI GGCC 3 cut(s) 27, 102, 600
Bsp119I TTCGAA 1 cut(s) 860
Bsp1286I GDGCHC 2 cut(s) 148, 248
Bsp143I GATC 1 cut(s) 460
BspACI CCGC 1 cut(s) 163
BspANI GGCC 3 cut(s) 27, 102, 600
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 88, 355
BspMI ACCTGC 1 cut(s) 261
BspT104I TTCGAA 1 cut(s) 860
BspT107I GGYRCC 2 cut(s) 86, 353
BsrDI GCAATG 1 cut(s) 594
BsrFI RCCGGY 1 cut(s) 733
BsrI ACTGG 1 cut(s) 257
BssAI RCCGGY 1 cut(s) 733
BssECI CCNNGG 2 cut(s) 28, 746
BssMI GATC 1 cut(s) 460
Bst2UI CCWGG 3 cut(s) 14, 186, 748
BstBI TTCGAA 1 cut(s) 860
BstC8I GCNNGC 1 cut(s) 81
BstDEI CTNAG 1 cut(s) 147
BstDSI CCRYGG 1 cut(s) 28
BstEII GGTNACC 1 cut(s) 188
BstF5I GGATG 2 cut(s) 40, 542
BstKTI GATC 1 cut(s) 463
BstMBI GATC 1 cut(s) 460
BstMWI GCNNNNNNNGC 6 cut(s) 80, 293, 671, 695, 704, 734
BstNI CCWGG 3 cut(s) 14, 186, 748
BstNSI RCATGY 1 cut(s) 83
BstPI GGTNACC 1 cut(s) 188
BstSCI CCNGG 3 cut(s) 12, 184, 746
BstV1I GCAGC 5 cut(s) 141, 310, 313, 503, 673
BsuRI GGCC 3 cut(s) 27, 102, 600
BtgI CCRYGG 1 cut(s) 28
BtsCI GGATG 2 cut(s) 40, 542
BtsI GCAGTG 1 cut(s) 548
BtsIMutI CAGTG 1 cut(s) 548
BveI ACCTGC 1 cut(s) 261
Cac8I GCNNGC 1 cut(s) 81
CaiI CAGNNNCTG 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 733
Cfr13I GGNCC 2 cut(s) 101, 599
CsiI ACCWGGT 1 cut(s) 184
Csp6I GTAC 1 cut(s) 333
CviAII CATG 4 cut(s) 80, 241, 338, 476
CviQI GTAC 1 cut(s) 333
DdeI CTNAG 1 cut(s) 147
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
DraI TTTAAA 1 cut(s) 807
EaeI YGGCCR 1 cut(s) 25
Eco91I GGTNACC 1 cut(s) 188
EcoO109I RGGNCCY 1 cut(s) 599
EcoO65I GGTNACC 1 cut(s) 188
EcoRII CCWGG 3 cut(s) 12, 184, 746
FaeI CATG 4 cut(s) 83, 244, 341, 479
FaqI GGGAC 1 cut(s) 563
FatI CATG 4 cut(s) 79, 240, 337, 475
FauI CCCGC 1 cut(s) 170
FauNDI CATATG 1 cut(s) 196
Fnu4HI GCNGC 5 cut(s) 155, 324, 327, 492, 687
FokI GGATG 2 cut(s) 47, 549
Fsp4HI GCNGC 5 cut(s) 155, 324, 327, 492, 687
FspBI CTAG 3 cut(s) 53, 369, 886
GluI GCNGC 5 cut(s) 155, 324, 327, 492, 687
HaeIII GGCC 3 cut(s) 27, 102, 600
HapII CCGG 1 cut(s) 734
Hin1II CATG 4 cut(s) 83, 244, 341, 479
HinfI GANTC 2 cut(s) 551, 862
HpaII CCGG 1 cut(s) 734
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 3 cut(s) 213, 335, 481
Hpy188I TCNGA 2 cut(s) 228, 402
Hpy188III TCNNGA 1 cut(s) 178
Hpy8I GTNNAC 3 cut(s) 213, 335, 481
HpyAV CCTTC 4 cut(s) 75, 482, 524, 861
HpyCH4V TGCA 2 cut(s) 9, 689
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 293, 671, 695, 704, 734
HpyF3I CTNAG 1 cut(s) 147
Hsp92II CATG 4 cut(s) 83, 244, 341, 479
Kzo9I GATC 1 cut(s) 460
LmnI GCTCC 1 cut(s) 584
Lsp1109I GCAGC 5 cut(s) 141, 310, 313, 503, 673
LweI GCATC 2 cut(s) 503, 746
MabI ACCWGGT 1 cut(s) 184
MaeI CTAG 3 cut(s) 53, 369, 886
MaeIII GTNAC 1 cut(s) 188
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 5 cut(s) 212, 635, 732, 869, 872
MhlI GDGCHC 2 cut(s) 148, 248
MlsI TGGCCA 1 cut(s) 27
MluCI AATT 3 cut(s) 204, 386, 637
MluNI TGGCCA 1 cut(s) 27
MlyI GAGTC 1 cut(s) 545
MmeI TCCRAC 2 cut(s) 574, 594
MnlI CCTC 7 cut(s) 56, 527, 540, 574, 590, 624, 770
Mox20I TGGCCA 1 cut(s) 27
MroXI GAANNNNTTC 1 cut(s) 204
MscI TGGCCA 1 cut(s) 27
MseI TTAA 3 cut(s) 273, 318, 806
MslI CAYNNNNRTG 4 cut(s) 33, 84, 239, 351
Msp20I TGGCCA 1 cut(s) 27
MspI CCGG 1 cut(s) 734
MspR9I CCNGG 3 cut(s) 14, 186, 748
MvaI CCWGG 3 cut(s) 14, 186, 748
MwoI GCNNNNNNNGC 6 cut(s) 80, 293, 671, 695, 704, 734
NdeI CATATG 1 cut(s) 196
NdeII GATC 1 cut(s) 460
NlaIII CATG 4 cut(s) 83, 244, 341, 479
NlaIV GGNNCC 2 cut(s) 88, 355
NmeAIII GCCGAG 1 cut(s) 396
NspI RCATGY 1 cut(s) 83
NspV TTCGAA 1 cut(s) 860
PaeI GCATGC 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 1 cut(s) 862
PkrI GCNGC 5 cut(s) 156, 325, 328, 493, 688
PleI GAGTC 1 cut(s) 545
PpsI GAGTC 1 cut(s) 545
PsiI TTATAA 1 cut(s) 507
Psp6I CCWGG 3 cut(s) 12, 184, 746
PspEI GGTNACC 1 cut(s) 188
PspGI CCWGG 3 cut(s) 12, 184, 746
PspN4I GGNNCC 2 cut(s) 88, 355
PspPI GGNCC 2 cut(s) 101, 599
PstNI CAGNNNCTG 1 cut(s) 154
RsaI GTAC 1 cut(s) 334
RsaNI GTAC 1 cut(s) 333
RseI CAYNNNNRTG 4 cut(s) 33, 84, 239, 351
SaqAI TTAA 3 cut(s) 273, 318, 806
SatI GCNGC 5 cut(s) 155, 324, 327, 492, 687
Sau3AI GATC 1 cut(s) 460
Sau96I GGNCC 2 cut(s) 101, 599
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 3 cut(s) 14, 186, 748
SduI GDGCHC 2 cut(s) 148, 248
SexAI ACCWGGT 1 cut(s) 184
SfaNI GCATC 2 cut(s) 503, 746
SfuI TTCGAA 1 cut(s) 860
SmiMI CAYNNNNRTG 4 cut(s) 33, 84, 239, 351
SphI GCATGC 1 cut(s) 83
Sse9I AATT 3 cut(s) 204, 386, 637
SsiI CCGC 1 cut(s) 163
SspMI CTAG 3 cut(s) 53, 369, 886
StyD4I CCNGG 3 cut(s) 12, 184, 746
TaqI TCGA 1 cut(s) 860
TasI AATT 3 cut(s) 204, 386, 637
TfiI GAWTC 1 cut(s) 862
Tru1I TTAA 3 cut(s) 273, 318, 806
Tru9I TTAA 3 cut(s) 273, 318, 806
TscAI CASTG 1 cut(s) 548
TseI GCWGC 5 cut(s) 154, 323, 326, 491, 686
TspDTI ATGAA 4 cut(s) 213, 229, 464, 773
TspRI CASTG 1 cut(s) 548
XapI RAATTY 2 cut(s) 204, 386
XceI RCATGY 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 3 cut(s) 53, 369, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.