Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0018073

Physical Location & Seq

Genomic Coordinates Forward (+)

33313075 .. 33318481

Gene Structure 5407 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0231300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3783 bp

ATGGCTCTCGTCAGATATCAATTAGAAGCTGCTTCCCCTTCCACTTCCGCTTCTCCTTCTCAGTTCAGCTACGACGTGTTCTTGAGTTTCAGAGGTGAAGACACCCGTAAGAACTTCACTGATCACCTCTACACCGCCTTGACCGGAGCAGGACTTCGCACTTTCCGAGACGACGATGAACTTCCCAGAGGAGAAGACATCAAACCAGAGCTGGAGAGAGCCATCCAACTCTCGCGAAGTTCCGTCATCGTGTTTTCTAAAGACTACGCCTCTTCCAAATGGTGCCTCGACGAGCTTTGCATGATCCTTCAACACAGAACGGCCTCCGATCACATCGTTTTGCCGGTCTTCTACGACATCGATCCATCTCACATCAGAAAGCAGACGGGGAGCGTTGCGATCGCATTTGCCAGACACCAGAAGAAGAGTTCTTTAGAAAAGTTGAATGCATGGAGGACAGCACTTGCGCAAGTTGCAGACCTTGCAGGAATGGTGCTCAAAAATGAAGCTGACGGGCACGAGTCAAAGTTCATTCAGAAAATTGTGAAAGTGATTGAGGAGAAATTATGTCGCCCACGATTGAGTGTTGCTTCTCATCTGATTGGAATTCATGATCGAGTCGAAAATATTAATTCATGGTTACAAGATGGATCATCTGGTGTTGGGGTATATTTAATTTATGGCATTGGGGGAATAGGAAAGACAACCATTGCACAAGTTGTTTATAATCTAAATTTTAGAAGATTTGAAGGAAGGAGTTTCCTTGAAAATATCAGAGAGACCTCACAAGGAACCATTGGCTTAATTCAACTCCAGAGACAACTTCTTTCTGATATTTTGGGTGGCAGAAAAGTGAAAATACAGAGTGTTAGTCAGGGAATAAGTAAAATTAGATATGCCATAAACTCTAAAAAAGTTCTTATTGTACTCGATGATGTGGATCATATGAGCCAATTCAATGCAATACTAAGGATGCGAGATTCGTTTCATCCGGGAAGTAAAATTATTGTAACAAGTAGGGATGTGGGGATTCTAGAGGCTTGTCGAGCTGATAAGGTGCATAATGTTAAACCTTTAAATAGGGTTGAATCATTGGAGTTGTTTAGTAGGCATGCTTTTGGACAGGACCATCCCATTGAAGGTTACAGGGAACTTTCACAATCAGTAGTACGCCACAGCGGTGGACTTCCATTAGCTCTTCAAACCTTGGGTTCCTCTCTCTGTGGGAAAAGTACAGATGTATGGGAAAGTGCATTAAAGAAATTGAGAGCTATTCCAAACAATGAAATCCTACATCAGCTGAGAATAAGCTACGACTCTTTACCAGATGACCATGACAAAGATTTATTCCTCCATATTGCATGTTTCTTTATTGGAAAGGGCAAGGATGTCATCGTTAGGATACTAGATGAATGCGAGTTCTACACAATTGTTGGCATTCAAAATCTCATTGATAGGTGCTTGGTAACCTTAGACGAGAATAATGTAGTCAAGATGCATCAAATGATTCGTGACATGGGAAGAGAAATTGTTCGCCTAGAATCAAAGTATCCAGAGAAACGTAGTAGATTATGGCATCATAAGGATTCATTCAATGTACTGAAAGAAAAGAGTGGTTCAAAAAAAATTGAAGGTTTAGCTCTGGACATGCAAATGTACCTGGCGGACATCCCCTCGAGCAATTCAAACAAGGTAACTCTGGAAACCAATGCATTTACAGGGATGCGCAGACTAAGATTACTCCAGCTTAGTCATGTACAACTTAATGGATGTTATGAAGAATTTCCTGATGGATTGAGATGGTTGTGTTGGGTTAAATTTCCTTTGGAGTCTATGCCCATTGATTTTCCTTTGGAGAGCCTGGTTGTTCTTGAATTGCAGTATAGCAGCTTGAAGCGATTTTGGAAGGGAACAAAAAGTCTTCCATCATTGAAAATTCTTGATCTGAGCCATTCTAGTGGCCTTTCTGAAACTGCTGATTTCTCAAATTTCCACAATTTAGAGAAATTGATCCTTGTAGATTGTGCTTGCCTTCTTGATGTCCATGAATCCATATGCACACTTCAGAAACTTGTTTACTTGAATATTAGAGATTGCAAAAAGATCAGGAAGCTCCCAAAGAACCTTTCTAGGCTAAAATTACTTGAAACGCTTATCATATCTGGTTGCTCAAGTCTTAACGAGTTTCCAGTGGACATGAGGAATATGGAATCTCTGAAAAAGCTTGAAGCAGATGAAATTCCTTTTAATCAAGTACTAGCTACTACAGGGGAGGTCAAATCATTGCTGATACATAAAACTATACATAATTTTTGGGATTCTGTCCCCTGCACTTTAGTTAATTTGAGTCTTGCAGACTGCAATCTTTTTGAAGATGCATTTCCTAGGGATTTAAGTAACTTATCCTCATTGCAAAGATTGGATCTTAGCGGTAATCCAATTTGTAGCTTACCAGATTGTGTCCGAGATCTTAGAGGCCTCGATCATCTTGTATTTTGGAATTGTTCGAGTCTCAAATCGCTTGTAGCGCTACCAGGAGTGAGAGAATTGGATACTTCCTACTGTGAATCATTGGAAAAAATAACATTTCAGTCACTTTCTTGTTTTCCAAAGAACATAGTTCTTTTTGGTGATGGATTAAAAATAGTTGAGATTGAATATTGGTACAAGATGGAAGCAATTGGAAGAGTGGATGAAGAGATGATCAATCTGTTGGGTTTGTGCAACTTGGAGTCCATAGGAGCCATTAGGATGTTTGCATTCCATATATACGATCTGGATGAGGCGACATTTGTTCTTCCCGTCCAGGGAGTGCATGAATATGGTATATTCAGCACATATATTCCTGGAAACGAAACCGAGGTACCAGGAACATTCAGCTATAAAATTAAGGGGTCATCCTCACTATCTTTTACTGTGGTTTTAGTTCCTAATCTCAGGTTCCGAGGCTTCAATATCTTCTGTGTTTATTATGGAGAGAGGGCCCAGACTTTAGGCACTTATGACAATATCCCACCAGTAATTATTCAAGTATGTAATAATAGCAAGGGTCTGAAGTGGATCTATGGACCGACATGCTACGGCATTCCAAGTGACAAAAAAGACGTGATATGGTTGAGCCATTGGAAGTTTGGGGTTCAACAATTCGAAGGGGGCGATGAAGTGGCTATTTCAGTGTTTACAATACGTGGGATTGAGGTAAAAGAATGGGGAATTCAGATTGTGCATGAGGAAGAAGATAACATGAGTAGCCAACACAACACCAGTAGAGATCCTTGTTATGATCCAGCACACGATAATGAGGTGATTGGTAATATTGGTGGAGAATTGTCAGATTTATATGAGGTCATGCCAGGAATATACTTTCTCTACGGTGGACCTTTTTTGTTGGATCACAGATATACTTACAATAATTGGAAAACACAAGGTTATTACAATGACTTCATTGAAGACTCTGATAAAGAATCTGCAGAAGAAGGGAAGCAGGGGGACGGGATGCTTGCGTCCACAGAAGAAGCTGAAGCAGAAACAGGCATCAATACCTTCTGCGGTTATTCTTGTTATTCAAATGTTGATGTCATTGGTGGAGATTTGTCAGAATATAGGGTGATGCTGAGAAGAATACTTCCTCTGCATTCAATGACATCATTTGAGACTGAGAGATATGTTGAATCACTGACAGCAGGGTCTGCTACCAAGTTTGAACATTATCCTGGGACAAGCTACTCAAACCTCTATTGGCATGCTCATGAAAACGGGGTGAATGTGAGTCACTTTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031090 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

1260

Amino Acids

142.63

Weight (kDa)

5.87

Isoelectric Point (pI)

42.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	24 - 189	2.2e-47	TIR domain
TIR_2	PF13676	26 - 138	2.8e-16	TIR domain
NB-ARC	PF00931	205 - 375	5.2e-20	NB-ARC domain
ATPase_2	PF01637	208 - 320	4.6e-06	ATPase domain predominantly from Archaea
WHD_ROQ1	PF23282	444 - 514	1.7e-18	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	656 - 746	1.4e-07	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018073)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g13390
rosa_chinensis	RchiOBHm_Chr1g0352711
rosa_laevigata	RLG00000028317
rosa_multiflora	Rmu_sc0006445.1_g000001
rosa_roxburghii	Rroxscaffold_4G00302420
rosa_rugosa	Rorug01G0231300 Rorug01G0231400
rosa_samantha	Rh1AG244400 Rh1BG215200 Rh1CG228200 Rh1DG241200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	726
AasI	GACNNNNNNGTC	1 cut(s)	3685
Acc16I	TGCGCA	2 cut(s)	468, 1727
Acc65I	GGTACC	1 cut(s)	2863
AccB1I	GGYRCC	2 cut(s)	282, 2863
AccII	CGCG	1 cut(s)	235
AciI	CCGC	6 cut(s)	48, 135, 1179, 1664, 2430, 3549
AcsI	RAATTY	8 cut(s)	606, 733, 1781, 1817, 1935, 1987, 2238, 3213
AcuI	CTGAAG	3 cut(s)	2048, 3074, 3540
AfaI	GTAC	9 cut(s)	927, 1170, 1234, 1599, 1658, 1758, 2256, 2666, 2865
AfeI	AGCGCT	1 cut(s)	2529
AfiI	CCNNNNNNNGG	2 cut(s)	1139, 2807
AflIII	ACRYGT	1 cut(s)	75
AjiI	CACGTC	2 cut(s)	76, 3106
AjnI	CCWGG	8 cut(s)	1659, 1860, 2533, 2805, 2845, 2866, 3352, 3712
AjuI	GAANNNNNNNTTGG	2 cut(s)	2110, 2142
AleI	CACNNNNGTG	1 cut(s)	1179
Alw21I	GWGCWC	1 cut(s)	498
Alw26I	GTCTC	5 cut(s)	162, 773, 811, 2516, 3647
AlwNI	CAGNNNCTG	1 cut(s)	3689
Ama87I	CYCGRG	1 cut(s)	1675
Aor51HI	AGCGCT	1 cut(s)	2529
AoxI	GGCC	4 cut(s)	321, 1960, 2476, 2982
ApaI	GGGCCC	1 cut(s)	2986
ApeKI	GCWGC	2 cut(s)	29, 1887
ApoI	RAATTY	8 cut(s)	606, 733, 1781, 1817, 1935, 1987, 2238, 3213
AseI	ATTAAT	1 cut(s)	630
AsiSI	GCGATCGC	1 cut(s)	402
Asp700I	GAANNNNTTC	2 cut(s)	1530, 1782
Asp718I	GGTACC	1 cut(s)	2863
AspA2I	CCTAGG	1 cut(s)	2384
AspLEI	GCGC	3 cut(s)	469, 1728, 2530
AspS9I	GGNCC	5 cut(s)	1126, 2982, 2983, 3068, 3377
AsuC2I	CCSGG	1 cut(s)	993
AsuHPI	GGTGA	6 cut(s)	107, 116, 2642, 3316, 3619, 3772
AsuII	TTCGAA	1 cut(s)	3147
AvaI	CYCGRG	1 cut(s)	1675
AvaII	GGWCC	3 cut(s)	1126, 3068, 3377
AvrII	CCTAGG	1 cut(s)	2384
BaeGI	GKGCMC	2 cut(s)	519, 2986
BaeI	ACNNNNGTAYC	2 cut(s)	2847, 2880
BanI	GGYRCC	2 cut(s)	282, 2863
BanII	GRGCYC	1 cut(s)	2986
BauI	CACGAG	1 cut(s)	518
BbsI	GAAGAC	5 cut(s)	105, 201, 340, 1913, 3456
Bbv12I	GWGCWC	1 cut(s)	498
BbvI	GCAGC	2 cut(s)	16, 1899
BccI	CCATC	9 cut(s)	230, 373, 641, 1137, 1784, 1794, 1933, 2627, 2665
BceAI	ACGGC	2 cut(s)	336, 3097
BciT130I	CCWGG	8 cut(s)	1661, 1862, 2535, 2807, 2847, 2868, 3354, 3714
BciVI	GTATCC	3 cut(s)	1395, 1560, 2545
BclI	TGATCA	2 cut(s)	121, 2703
BcnI	CCSGG	1 cut(s)	993
BcoDI	GTCTC	5 cut(s)	162, 773, 811, 2516, 3647
BfaI	CTAG	7 cut(s)	1034, 1406, 1538, 1956, 2130, 2258, 2385
BfmI	CTRYAG	2 cut(s)	2265, 3468
BfoI	RGCGCY	1 cut(s)	2531
BfuI	GTATCC	3 cut(s)	1395, 1560, 2545
BglII	AGATCT	1 cut(s)	2467
BisI	GCNGC	2 cut(s)	30, 1888
BlnI	CCTAGG	1 cut(s)	2384
BlsI	GCNGC	2 cut(s)	31, 1889
BmcAI	AGTACT	1 cut(s)	2256
Bme1390I	CCNGG	9 cut(s)	993, 1661, 1862, 2535, 2807, 2847, 2868, 3354, 3714
Bme18I	GGWCC	3 cut(s)	1126, 3068, 3377
BmeT110I	CYCGRG	1 cut(s)	1675
BmgBI	CACGTC	2 cut(s)	76, 3106
BmgT120I	GGNCC	5 cut(s)	1126, 2982, 2983, 3068, 3377
BmiI	GGNNCC	7 cut(s)	284, 793, 1213, 2743, 2865, 2942, 2984
BmrFI	CCNGG	9 cut(s)	993, 1661, 1862, 2535, 2807, 2847, 2868, 3354, 3714
BmsI	GCATC	9 cut(s)	963, 1485, 1507, 1585, 1713, 2365, 3486, 3543, 3600
BpiI	GAAGAC	5 cut(s)	105, 201, 340, 1913, 3456
BpmI	CTGGAG	3 cut(s)	233, 797, 1727
Bpu14I	TTCGAA	1 cut(s)	3147
BpuEI	CTTGAG	2 cut(s)	103, 2155
BpuMI	CCSGG	1 cut(s)	993
Bsa29I	ATCGAT	1 cut(s)	360
BsaAI	YACGTR	1 cut(s)	3188
BsaBI	GATNNNNATC	1 cut(s)	939
BsaI	GGTCTC	1 cut(s)	773
BsaJI	CCNNGG	6 cut(s)	1206, 2384, 2806, 2859, 2944, 3713
BsaWI	WCCGGW	1 cut(s)	143
Bsc4I	CCNNNNNNNGG	2 cut(s)	1139, 2807
Bse118I	RCCGGY	1 cut(s)	343
Bse1I	ACTGG	3 cut(s)	2189, 3017, 3264
Bse3DI	GCAATG	3 cut(s)	708, 2282, 2408
Bse8I	GATNNNNATC	1 cut(s)	939
BseBI	CCWGG	8 cut(s)	1661, 1862, 2535, 2807, 2847, 2868, 3354, 3714
BseCI	ATCGAT	1 cut(s)	360
BseDI	CCNNGG	6 cut(s)	1206, 2384, 2806, 2859, 2944, 3713
BseJI	GATNNNNATC	1 cut(s)	939
BseLI	CCNNNNNNNGG	2 cut(s)	1139, 2807
BseMI	GCAATG	3 cut(s)	708, 2282, 2408
BseMII	CTCAG	6 cut(s)	74, 1292, 1937, 2950, 3605, 3648
BseNI	ACTGG	3 cut(s)	2189, 3017, 3264
BseRI	GAGGAG	2 cut(s)	204, 572
BseSI	GKGCMC	2 cut(s)	519, 2986
BseXI	GCAGC	2 cut(s)	16, 1899
BsgI	GTGCAG	1 cut(s)	2314
Bsh1236I	CGCG	1 cut(s)	235
Bsh1285I	CGRYCG	1 cut(s)	402
BshFI	GGCC	4 cut(s)	323, 1962, 2478, 2984
BshNI	GGYRCC	2 cut(s)	282, 2863
BshVI	ATCGAT	1 cut(s)	360
BsiEI	CGRYCG	1 cut(s)	402
BsiHKAI	GWGCWC	1 cut(s)	498
BsiHKCI	CYCGRG	1 cut(s)	1675
BsiSI	CCGG	3 cut(s)	144, 344, 992
BslFI	GGGAC	3 cut(s)	2309, 3503, 3730
BslI	CCNNNNNNNGG	2 cut(s)	1139, 2807
BsmAI	GTCTC	5 cut(s)	162, 773, 811, 2516, 3647
BsmBI	CGTCTC	1 cut(s)	162
BsmFI	GGGAC	3 cut(s)	2309, 3503, 3730
BsmI	GAATGC	6 cut(s)	451, 1418, 1437, 2759, 3084, 3634
BsnI	GGCC	4 cut(s)	323, 1962, 2478, 2984
Bso31I	GGTCTC	1 cut(s)	773
BsoBI	CYCGRG	1 cut(s)	1675
Bsp119I	TTCGAA	1 cut(s)	3147
Bsp120I	GGGCCC	1 cut(s)	2982
Bsp1286I	GDGCHC	3 cut(s)	498, 519, 2986
Bsp1407I	TGTACA	1 cut(s)	1756
Bsp68I	TCGCGA	1 cut(s)	235
BspACI	CCGC	6 cut(s)	48, 135, 1179, 1664, 2430, 3549
BspANI	GGCC	4 cut(s)	323, 1962, 2478, 2984
BspCNI	CTCAG	6 cut(s)	73, 1293, 1938, 2949, 3606, 3649
BspDI	ATCGAT	1 cut(s)	360
BspFNI	CGCG	1 cut(s)	235
BspHI	TCATGA	2 cut(s)	610, 3748
BspLI	GGNNCC	7 cut(s)	284, 793, 1213, 2743, 2865, 2942, 2984
BspMAI	CTGCAG	1 cut(s)	3472
BspQI	GCTCTTC	1 cut(s)	1203
BspT104I	TTCGAA	1 cut(s)	3147
BspT107I	GGYRCC	2 cut(s)	282, 2863
BspTNI	GGTCTC	1 cut(s)	773
BsrDI	GCAATG	3 cut(s)	708, 2282, 2408
BsrFI	RCCGGY	1 cut(s)	343
BsrGI	TGTACA	1 cut(s)	1756
BsrI	ACTGG	3 cut(s)	2189, 3017, 3264
BssAI	RCCGGY	1 cut(s)	343
BssECI	CCNNGG	6 cut(s)	1206, 2384, 2806, 2859, 2944, 3713
BssSI	CACGAG	1 cut(s)	518
BssT1I	CCWWGG	2 cut(s)	1206, 2384
Bst2BI	CACGAG	1 cut(s)	518
Bst2UI	CCWGG	8 cut(s)	1661, 1862, 2535, 2807, 2847, 2868, 3354, 3714
Bst4CI	ACNGT	3 cut(s)	2564, 2917, 3374
Bst6I	CTCTTC	6 cut(s)	277, 419, 1203, 1516, 2680, 2691
BstAPI	GCANNNNNTGC	2 cut(s)	482, 3689
BstAUI	TGTACA	1 cut(s)	1756
BstBAI	YACGTR	1 cut(s)	3188
BstBI	TTCGAA	1 cut(s)	3147
BstC8I	GCNNGC	4 cut(s)	1113, 2029, 3501, 3744
BstEII	GGTNACC	1 cut(s)	1465
BstFNI	CGCG	1 cut(s)	235
BstH2I	RGCGCY	1 cut(s)	2531
BstHHI	GCGC	3 cut(s)	469, 1728, 2530
BstMAI	GTCTC	5 cut(s)	162, 773, 811, 2516, 3647
BstMCI	CGRYCG	1 cut(s)	402
BstMWI	GCNNNNNNNGC	5 cut(s)	473, 482, 1046, 2527, 3689
BstNI	CCWGG	8 cut(s)	1661, 1862, 2535, 2807, 2847, 2868, 3354, 3714
BstNSI	RCATGY	5 cut(s)	1115, 1365, 1651, 3078, 3746
BstPI	GGTNACC	1 cut(s)	1465
BstSCI	CCNGG	9 cut(s)	991, 1659, 1860, 2533, 2805, 2845, 2866, 3352, 3712
BstSFI	CTRYAG	2 cut(s)	2265, 3468
BstSLI	GKGCMC	2 cut(s)	519, 2986
BstUI	CGCG	1 cut(s)	235
BstV1I	GCAGC	2 cut(s)	16, 1899
BstV2I	GAAGAC	5 cut(s)	105, 201, 340, 1913, 3456
BstX2I	RGATCY	4 cut(s)	2422, 2467, 3060, 3271
BstXI	CCANNNNNNTGG	2 cut(s)	1181, 1958
BstYI	RGATCY	4 cut(s)	2422, 2467, 3060, 3271
Bsu15I	ATCGAT	1 cut(s)	360
BsuI	GTATCC	3 cut(s)	1395, 1560, 2545
BsuRI	GGCC	4 cut(s)	323, 1962, 2478, 2984
BsuTUI	ATCGAT	1 cut(s)	360
BtgZI	GCGATG	1 cut(s)	3171
BtrI	CACGTC	2 cut(s)	76, 3106
BtsIMutI	CAGTG	4 cut(s)	117, 2196, 3180, 3674
BtuMI	TCGCGA	1 cut(s)	235
Cac8I	GCNNGC	4 cut(s)	1113, 2029, 3501, 3744
CaiI	CAGNNNCTG	1 cut(s)	3689
CciI	TCATGA	2 cut(s)	610, 3748
CfoI	GCGC	3 cut(s)	469, 1728, 2530
Cfr10I	RCCGGY	1 cut(s)	343
Cfr13I	GGNCC	5 cut(s)	1126, 2982, 2983, 3068, 3377
ClaI	ATCGAT	1 cut(s)	360
CseI	GACGC	1 cut(s)	3492
Csp6I	GTAC	9 cut(s)	926, 1169, 1233, 1598, 1657, 1757, 2255, 2665, 2864
CviQI	GTAC	9 cut(s)	926, 1169, 1233, 1598, 1657, 1757, 2255, 2665, 2864
DraI	TTTAAA	1 cut(s)	1077
DrdI	GACNNNNNNGTC	1 cut(s)	3685
DseDI	GACNNNNNNGTC	1 cut(s)	3685
Eam1104I	CTCTTC	6 cut(s)	277, 419, 1203, 1516, 2680, 2691
EarI	CTCTTC	6 cut(s)	277, 419, 1203, 1516, 2680, 2691
EciI	GGCGGA	1 cut(s)	1679
Eco130I	CCWWGG	2 cut(s)	1206, 2384
Eco147I	AGGCCT	1 cut(s)	2478
Eco24I	GRGCYC	1 cut(s)	2986
Eco31I	GGTCTC	1 cut(s)	773
Eco32I	GATATC	1 cut(s)	17
Eco47I	GGWCC	3 cut(s)	1126, 3068, 3377
Eco47III	AGCGCT	1 cut(s)	2529
Eco57I	CTGAAG	3 cut(s)	2048, 3074, 3540
Eco88I	CYCGRG	1 cut(s)	1675
Eco91I	GGTNACC	1 cut(s)	1465
EcoO109I	RGGNCCY	1 cut(s)	2982
EcoO65I	GGTNACC	1 cut(s)	1465
EcoRI	GAATTC	2 cut(s)	606, 3213
EcoRII	CCWGG	8 cut(s)	1659, 1860, 2533, 2805, 2845, 2866, 3352, 3712
EcoRV	GATATC	1 cut(s)	17
EcoT14I	CCWWGG	2 cut(s)	1206, 2384
EcoT22I	ATGCAT	4 cut(s)	451, 1500, 1714, 2380
EcoT38I	GRGCYC	1 cut(s)	2986
ErhI	CCWWGG	2 cut(s)	1206, 2384
Esp3I	CGTCTC	1 cut(s)	162
FaqI	GGGAC	3 cut(s)	2309, 3503, 3730
FauNDI	CATATG	2 cut(s)	945, 2054
FbaI	TGATCA	2 cut(s)	121, 2703
Fnu4HI	GCNGC	2 cut(s)	30, 1888
FriOI	GRGCYC	1 cut(s)	2986
Fsp4HI	GCNGC	2 cut(s)	30, 1888
FspBI	CTAG	7 cut(s)	1034, 1406, 1538, 1956, 2130, 2258, 2385
FspI	TGCGCA	2 cut(s)	468, 1727
GlaI	GCGC	3 cut(s)	468, 1727, 2529
GluI	GCNGC	2 cut(s)	30, 1888
GsuI	CTGGAG	3 cut(s)	233, 797, 1727
HaeII	RGCGCY	1 cut(s)	2531
HaeIII	GGCC	4 cut(s)	323, 1962, 2478, 2984
HapII	CCGG	3 cut(s)	144, 344, 992
HgaI	GACGC	1 cut(s)	3492
HhaI	GCGC	3 cut(s)	469, 1728, 2530
Hin6I	GCGC	3 cut(s)	467, 1726, 2528
HinP1I	GCGC	3 cut(s)	467, 1726, 2528
HindIII	AAGCTT	1 cut(s)	2222
HpaII	CCGG	3 cut(s)	144, 344, 992
HphI	GGTGA	6 cut(s)	107, 116, 2642, 3316, 3619, 3772
Hpy166II	GTNNAC	6 cut(s)	1184, 2077, 2194, 3180, 3377, 3507
Hpy8I	GTNNAC	6 cut(s)	1184, 2077, 2194, 3180, 3377, 3507
Hpy99I	CGWCG	3 cut(s)	77, 176, 293
HpyCH4III	ACNGT	3 cut(s)	2564, 2917, 3374
HpyCH4IV	ACGT	4 cut(s)	75, 1561, 3105, 3187
HpyF10VI	GCNNNNNNNGC	5 cut(s)	473, 482, 1046, 2527, 3689
HpySE526I	ACGT	4 cut(s)	75, 1561, 3105, 3187
HspAI	GCGC	3 cut(s)	467, 1726, 2528
KpnI	GGTACC	1 cut(s)	2867
Ksp22I	TGATCA	2 cut(s)	121, 2703
LguI	GCTCTTC	1 cut(s)	1203
LmnI	GCTCC	4 cut(s)	146, 390, 2118, 2741
Lsp1109I	GCAGC	2 cut(s)	16, 1899
LweI	GCATC	9 cut(s)	963, 1485, 1507, 1585, 1713, 2365, 3486, 3543, 3600
MaeI	CTAG	7 cut(s)	1034, 1406, 1538, 1956, 2130, 2258, 2385
MaeII	ACGT	4 cut(s)	75, 1561, 3105, 3187
MfeI	CAATTG	2 cut(s)	1428, 2679
MflI	RGATCY	4 cut(s)	2422, 2467, 3060, 3271
MhlI	GDGCHC	3 cut(s)	498, 519, 2986
MlyI	GAGTC	9 cut(s)	530, 627, 1310, 1838, 2356, 2518, 2741, 3446, 3778
MmeI	TCCRAC	2 cut(s)	250, 3369
Mph1103I	ATGCAT	4 cut(s)	451, 1500, 1714, 2380
MroXI	GAANNNNTTC	2 cut(s)	1530, 1782
MslI	CAYNNNNRTG	7 cut(s)	1179, 1652, 1956, 2750, 2820, 3297, 3747
MspA1I	CMGCKG	2 cut(s)	1179, 1300
MspI	CCGG	3 cut(s)	144, 344, 992
MspR9I	CCNGG	9 cut(s)	993, 1661, 1862, 2535, 2807, 2847, 2868, 3354, 3714
MunI	CAATTG	2 cut(s)	1428, 2679
Mva1269I	GAATGC	6 cut(s)	451, 1418, 1437, 2759, 3084, 3634
MvaI	CCWGG	8 cut(s)	1661, 1862, 2535, 2807, 2847, 2868, 3354, 3714
MvnI	CGCG	1 cut(s)	235
MwoI	GCNNNNNNNGC	5 cut(s)	473, 482, 1046, 2527, 3689
NciI	CCSGG	1 cut(s)	993
NdeI	CATATG	2 cut(s)	945, 2054
NlaIV	GGNNCC	7 cut(s)	284, 793, 1213, 2743, 2865, 2942, 2984
NmuCI	GTSAC	4 cut(s)	1511, 2592, 3092, 3770
NruI	TCGCGA	1 cut(s)	235
NsbI	TGCGCA	2 cut(s)	468, 1727
NsiI	ATGCAT	4 cut(s)	451, 1500, 1714, 2380
NspI	RCATGY	5 cut(s)	1115, 1365, 1651, 3078, 3746
NspV	TTCGAA	1 cut(s)	3147
OliI	CACNNNNGTG	1 cut(s)	1179
PaeI	GCATGC	2 cut(s)	1115, 3746
PaeR7I	CTCGAG	1 cut(s)	1675
PagI	TCATGA	2 cut(s)	610, 3748
PceI	AGGCCT	1 cut(s)	2478
PciSI	GCTCTTC	1 cut(s)	1203
PcsI	WCGNNNNNNNCGW	2 cut(s)	163, 3153
PctI	GAATGC	6 cut(s)	451, 1418, 1437, 2759, 3084, 3634
PdmI	GAANNNNTTC	2 cut(s)	1530, 1782
PfoI	TCCNGGA	2 cut(s)	991, 2845
PkrI	GCNGC	2 cut(s)	31, 1889
Ple19I	CGATCG	1 cut(s)	402
PleI	GAGTC	9 cut(s)	529, 626, 1310, 1837, 2355, 2517, 2740, 3446, 3777
PpsI	GAGTC	9 cut(s)	529, 626, 1310, 1837, 2355, 2517, 2740, 3446, 3777
Ppu21I	YACGTR	1 cut(s)	3188
PshBI	ATTAAT	1 cut(s)	630
PsiI	TTATAA	1 cut(s)	726
Psp6I	CCWGG	8 cut(s)	1659, 1860, 2533, 2805, 2845, 2866, 3352, 3712
PspEI	GGTNACC	1 cut(s)	1465
PspGI	CCWGG	8 cut(s)	1659, 1860, 2533, 2805, 2845, 2866, 3352, 3712
PspN4I	GGNNCC	7 cut(s)	284, 793, 1213, 2743, 2865, 2942, 2984
PspOMI	GGGCCC	1 cut(s)	2982
PspPI	GGNCC	5 cut(s)	1126, 2982, 2983, 3068, 3377
PspXI	VCTCGAGB	1 cut(s)	1675
PstI	CTGCAG	1 cut(s)	3472
PstNI	CAGNNNCTG	1 cut(s)	3689
PsuI	RGATCY	4 cut(s)	2422, 2467, 3060, 3271
PvuI	CGATCG	1 cut(s)	402
PvuII	CAGCTG	1 cut(s)	1300
RgaI	GCGATCGC	1 cut(s)	402
RruI	TCGCGA	1 cut(s)	235
RsaI	GTAC	9 cut(s)	927, 1170, 1234, 1599, 1658, 1758, 2256, 2666, 2865
RsaNI	GTAC	9 cut(s)	926, 1169, 1233, 1598, 1657, 1757, 2255, 2665, 2864
RseI	CAYNNNNRTG	7 cut(s)	1179, 1652, 1956, 2750, 2820, 3297, 3747
SapI	GCTCTTC	1 cut(s)	1203
SatI	GCNGC	2 cut(s)	30, 1888
Sau96I	GGNCC	5 cut(s)	1126, 2982, 2983, 3068, 3377
ScaI	AGTACT	1 cut(s)	2256
SchI	GAGTC	9 cut(s)	530, 627, 1310, 1838, 2356, 2518, 2741, 3446, 3778
ScrFI	CCNGG	9 cut(s)	993, 1661, 1862, 2535, 2807, 2847, 2868, 3354, 3714
SduI	GDGCHC	3 cut(s)	498, 519, 2986
SfaAI	GCGATCGC	1 cut(s)	402
SfaNI	GCATC	9 cut(s)	963, 1485, 1507, 1585, 1713, 2365, 3486, 3543, 3600
SfcI	CTRYAG	2 cut(s)	2265, 3468
Sfr274I	CTCGAG	1 cut(s)	1675
SfuI	TTCGAA	1 cut(s)	3147
SgfI	GCGATCGC	1 cut(s)	402
SinI	GGWCC	3 cut(s)	1126, 3068, 3377
SlaI	CTCGAG	1 cut(s)	1675
SmiMI	CAYNNNNRTG	7 cut(s)	1179, 1652, 1956, 2750, 2820, 3297, 3747
SmlI	CTYRAG	3 cut(s)	82, 1675, 2170
SmoI	CTYRAG	3 cut(s)	82, 1675, 2170
SphI	GCATGC	2 cut(s)	1115, 3746
SseBI	AGGCCT	1 cut(s)	2478
SsiI	CCGC	6 cut(s)	48, 135, 1179, 1664, 2430, 3549
SspI	AATATT	4 cut(s)	628, 2086, 2660, 3316
SspMI	CTAG	7 cut(s)	1034, 1406, 1538, 1956, 2130, 2258, 2385
StuI	AGGCCT	1 cut(s)	2478
StyD4I	CCNGG	9 cut(s)	991, 1659, 1860, 2533, 2805, 2845, 2866, 3352, 3712
StyI	CCWWGG	2 cut(s)	1206, 2384
TaaI	ACNGT	3 cut(s)	2564, 2917, 3374
TaiI	ACGT	4 cut(s)	78, 1564, 3108, 3190
TaqII	GACCGA	1 cut(s)	3085
TatI	WGTACW	5 cut(s)	925, 1232, 1597, 1756, 2254
TscAI	CASTG	4 cut(s)	124, 2196, 3180, 3681
TseFI	GTSAC	4 cut(s)	1511, 2592, 3092, 3770
TseI	GCWGC	2 cut(s)	29, 1887
Tsp45I	GTSAC	4 cut(s)	1511, 2592, 3092, 3770
TspGWI	ACGGA	1 cut(s)	232
TspRI	CASTG	4 cut(s)	124, 2196, 3180, 3681
VpaK11BI	GGWCC	3 cut(s)	1126, 3068, 3377
VspI	ATTAAT	1 cut(s)	630
XapI	RAATTY	8 cut(s)	606, 733, 1781, 1817, 1935, 1987, 2238, 3213
XbaI	TCTAGA	1 cut(s)	1033
XceI	RCATGY	5 cut(s)	1115, 1365, 1651, 3078, 3746
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3128
XhoI	CTCGAG	1 cut(s)	1675
XmaJI	CCTAGG	1 cut(s)	2384
XmnI	GAANNNNTTC	2 cut(s)	1530, 1782
XspI	CTAG	7 cut(s)	1034, 1406, 1538, 1956, 2130, 2258, 2385
ZrmI	AGTACT	1 cut(s)	2256
Zsp2I	ATGCAT	4 cut(s)	451, 1500, 1714, 2380

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.