Rh1CG228200

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0018073
Physical Location & Seq
Forward (+)
48220557 .. 48223382
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG228200.1

Sequence Viewer

Length: 1086 bp
ATGCAGGCGGCATGGGACACGAGAATGGCAATGGACCCCATGCCCAGGGTGGGCTACTCAATCGCCGCCGACGAGCTCCTCCGTAACCGGCGGCTCATGCGGCGGGCGCCGGCCCCAGTACGCGGCACCGGTTGGCTCCTGCGCCGTGTGATGAGGCTGAATGAGCAGCCGGTTCGGGCCGGGGAGATTTCCGGGGAGGAGCTGCAAGTGGGCCACAGCAGCTCCTCCTTGAAGTTGTCTATAATTCTGGACTTGCTGTGGAATCTGTCGTTTGTGGTGGTTGGGATCACAATGCTGGGGCTGAGCGCGGCGGAGAAGCCTCCGGTGCCTTTGAGGTTTTGGGCGAGTGGATATGTGTTGCTCTCTGCAGTTCATATTGGGTGTGTGGTTGTAGGCAAGAGAAGAGAGTTGGGTTTCGGCTCAGAGGGTTGGAGTTCTGGAATTGAAGGCGAAAGTGATGAAAGTGAGCAGGTCCGGGCTGAGGAGTATAGAAGAGATTTTGCAAAGACTGTGAGTACGGCCAATTCCATGGCTTCAGTGGTTTGGTGGCTCGCTGGATTCTACTGGGTGGTTGGTGGCGAAGCTTTAAGTGACGCTCCTCGTCTATTCTGGCTTTGTGTTGCATTTCTACTGTGTGATATCATGATCTTCATTTTATGTGTTGCTGCCACATGTCTTTATTGTCTCTCGGTCTGCTGTTGTCTGCCGTGCATACTTGCAATCTTACCTGCTTTGACAAATAAGGATGGGGCTACAGAGGACGAGATTAATAGCTTACCCAAGTTCAAATTCTGCAGGATTGGTGATTTTAGGATGGTTAATGGTCAGTTCCAAGAATCATCTGGAGGATCTACAACTGAATTTGATGCCAGTGCACCAAATGAGCATGTTGGTTCCCAGGATTATGCTGACTGCTGCATCTGCCTGGAAGTCTACCAGAATGGAGAAGAACTGCGCCAACTGCCTTGCCATCATCATTTTCACGGTGCCTGCATAGATAAATGGTTACGCAAGAGAACTACTTGCCCCATGTGCAAGTTCGACTTCTTGAAGCCTATATACGGAGATGGTAGGACAATCGTGTGA

Protein Analysis

361

Amino Acids

40.17

Weight (kDa)

6.02

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 302 - 346 2.2e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 304 - 346 2.3e-14 Ring finger domain
zf-RING_11 PF17123 304 - 332 2.6e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 305 - 345 2.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 305 - 345 2.8e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g13390
rosa_chinensis RchiOBHm_Chr1g0352711
rosa_laevigata RLG00000028317
rosa_multiflora Rmu_sc0006445.1_g000001
rosa_roxburghii Rroxscaffold_4G00302420
rosa_rugosa Rorug01G0231300 Rorug01G0231400
rosa_samantha Rh1AG244400 Rh1BG215200 Rh1CG228200 Rh1DG241200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 460, 736
AccB1I GGYRCC 4 cut(s) 106, 125, 325, 986
AccI GTMKAC 1 cut(s) 933
AccII CGCG 2 cut(s) 123, 308
AciI CCGC 8 cut(s) 8, 66, 91, 100, 103, 123, 308, 311
AclWI GGATC 2 cut(s) 293, 856
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 2 cut(s) 788, 860
AcuI CTGAAG 1 cut(s) 519
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 2 cut(s) 120, 517
AfiI CCNNNNNNNGG 5 cut(s) 45, 50, 122, 481, 1061
AflIII ACRYGT 1 cut(s) 671
AgeI ACCGGT 1 cut(s) 128
AgsI TTSAA 4 cut(s) 232, 446, 787, 1051
AjnI CCWGG 3 cut(s) 44, 897, 924
AjuI GAANNNNNNNTTGG 2 cut(s) 773, 805
AluBI AGCT 5 cut(s) 76, 202, 222, 584, 774
AluI AGCT 5 cut(s) 76, 202, 222, 584, 774
Alw21I GWGCWC 2 cut(s) 78, 877
Alw26I GTCTC 1 cut(s) 689
Alw44I GTGCAC 1 cut(s) 873
AlwI GGATC 2 cut(s) 293, 856
AoxI GGCC 4 cut(s) 111, 177, 211, 519
ApaLI GTGCAC 1 cut(s) 873
ApeKI GCWGC 5 cut(s) 166, 202, 219, 665, 915
ApoI RAATTY 2 cut(s) 788, 860
AseI ATTAAT 1 cut(s) 768
AsiGI ACCGGT 1 cut(s) 128
AspLEI GCGC 4 cut(s) 109, 144, 308, 957
AspS9I GGNCC 5 cut(s) 34, 112, 177, 211, 472
AsuC2I CCSGG 3 cut(s) 181, 193, 476
AsuHPI GGTGA 1 cut(s) 815
AvaII GGWCC 2 cut(s) 34, 472
BaeGI GKGCMC 1 cut(s) 877
BanI GGYRCC 4 cut(s) 106, 125, 325, 986
BanII GRGCYC 1 cut(s) 78
BarI GAAGNNNNNNTAC 2 cut(s) 1043, 1075
BauI CACGAG 1 cut(s) 19
Bbv12I GWGCWC 2 cut(s) 78, 877
BbvCI CCTCAGC 1 cut(s) 480
BbvI GCAGC 5 cut(s) 178, 189, 231, 652, 902
BccI CCATC 4 cut(s) 740, 808, 978, 1061
BceAI ACGGC 3 cut(s) 129, 534, 691
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 3 cut(s) 46, 899, 926
BcnI CCSGG 3 cut(s) 181, 193, 476
BcoDI GTCTC 1 cut(s) 689
BfmI CTRYAG 3 cut(s) 366, 753, 793
BfoI RGCGCY 1 cut(s) 110
BfuAI ACCTGC 2 cut(s) 460, 736
BlpI GCTNAGC 1 cut(s) 302
Bme1390I CCNGG 6 cut(s) 46, 181, 193, 476, 899, 926
Bme18I GGWCC 2 cut(s) 34, 472
BmgT120I GGNCC 5 cut(s) 34, 112, 177, 211, 472
BmiI GGNNCC 8 cut(s) 36, 108, 114, 127, 137, 327, 895, 988
BmrFI CCNGG 6 cut(s) 46, 181, 193, 476, 899, 926
BmrI ACTGGG 2 cut(s) 110, 574
BmsI GCATC 2 cut(s) 856, 927
BmuI ACTGGG 2 cut(s) 110, 574
BpmI CTGGAG 1 cut(s) 864
Bpu10I CCTNAGC 1 cut(s) 480
Bpu1102I GCTNAGC 1 cut(s) 302
BpuMI CCSGG 3 cut(s) 181, 193, 476
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 6 cut(s) 44, 45, 180, 192, 528, 897
BsaWI WCCGGW 2 cut(s) 128, 322
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 50, 122, 481, 1061
Bse118I RCCGGY 4 cut(s) 87, 109, 128, 169
Bse1I ACTGG 3 cut(s) 116, 569, 870
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 3 cut(s) 46, 899, 926
BseDI CCNNGG 6 cut(s) 44, 45, 180, 192, 528, 897
BseGI GGATG 2 cut(s) 751, 819
BseLI CCNNNNNNNGG 5 cut(s) 45, 50, 122, 481, 1061
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 3 cut(s) 293, 435, 471
BseNI ACTGG 3 cut(s) 116, 569, 870
BseRI GAGGAG 5 cut(s) 68, 212, 214, 497, 588
BseSI GKGCMC 1 cut(s) 877
BseXI GCAGC 5 cut(s) 178, 189, 231, 652, 902
BseYI CCCAGC 1 cut(s) 295
Bsh1236I CGCG 2 cut(s) 123, 308
BshFI GGCC 4 cut(s) 113, 179, 213, 521
BshNI GGYRCC 4 cut(s) 106, 125, 325, 986
BshTI ACCGGT 1 cut(s) 128
BsiHKAI GWGCWC 2 cut(s) 78, 877
BsiSI CCGG 8 cut(s) 88, 110, 129, 170, 180, 192, 323, 475
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 5 cut(s) 45, 50, 122, 481, 1061
BsmAI GTCTC 1 cut(s) 689
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 4 cut(s) 113, 179, 213, 521
Bsp1286I GDGCHC 2 cut(s) 78, 877
Bsp143I GATC 3 cut(s) 285, 645, 848
Bsp1720I GCTNAGC 1 cut(s) 302
Bsp19I CCATGG 1 cut(s) 528
BspACI CCGC 8 cut(s) 8, 66, 91, 100, 103, 123, 308, 311
BspANI GGCC 4 cut(s) 113, 179, 213, 521
BspCNI CTCAG 3 cut(s) 294, 434, 472
BspFNI CGCG 2 cut(s) 123, 308
BspHI TCATGA 1 cut(s) 642
BspLI GGNNCC 8 cut(s) 36, 108, 114, 127, 137, 327, 895, 988
BspMAI CTGCAG 2 cut(s) 370, 797
BspMI ACCTGC 2 cut(s) 460, 736
BspPI GGATC 2 cut(s) 293, 856
BspT107I GGYRCC 4 cut(s) 106, 125, 325, 986
BsrDI GCAATG 1 cut(s) 36
BsrFI RCCGGY 4 cut(s) 87, 109, 128, 169
BsrI ACTGG 3 cut(s) 116, 569, 870
BssAI RCCGGY 4 cut(s) 87, 109, 128, 169
BssECI CCNNGG 6 cut(s) 44, 45, 180, 192, 528, 897
BssMI GATC 3 cut(s) 285, 645, 848
BssNI GRCGYC 1 cut(s) 107
BssSI CACGAG 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 528
Bst2BI CACGAG 1 cut(s) 19
Bst2UI CCWGG 3 cut(s) 46, 899, 926
Bst4CI ACNGT 3 cut(s) 511, 633, 986
Bst6I CTCTTC 2 cut(s) 397, 487
BstACI GRCGYC 1 cut(s) 107
BstC8I GCNNGC 5 cut(s) 6, 105, 111, 552, 991
BstDEI CTNAG 3 cut(s) 302, 421, 480
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 2 cut(s) 751, 819
BstFNI CGCG 2 cut(s) 123, 308
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 4 cut(s) 109, 144, 308, 957
BstKTI GATC 3 cut(s) 288, 648, 851
BstMAI GTCTC 1 cut(s) 689
BstMBI GATC 3 cut(s) 285, 645, 848
BstMWI GCNNNNNNNGC 9 cut(s) 97, 100, 106, 163, 219, 325, 921, 961, 1032
BstNI CCWGG 3 cut(s) 46, 899, 926
BstNSI RCATGY 2 cut(s) 675, 890
BstSCI CCNGG 6 cut(s) 44, 179, 191, 474, 897, 924
BstSFI CTRYAG 3 cut(s) 366, 753, 793
BstSLI GKGCMC 1 cut(s) 877
BstUI CGCG 2 cut(s) 123, 308
BstV1I GCAGC 5 cut(s) 178, 189, 231, 652, 902
BstX2I RGATCY 1 cut(s) 848
BstXI CCANNNNNNTGG 1 cut(s) 529
BstYI RGATCY 1 cut(s) 848
BsuRI GGCC 4 cut(s) 113, 179, 213, 521
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 2 cut(s) 751, 819
BtsIMutI CAGTG 2 cut(s) 543, 877
BveI ACCTGC 2 cut(s) 460, 736
Cac8I GCNNGC 5 cut(s) 6, 105, 111, 552, 991
CciI TCATGA 1 cut(s) 642
CfoI GCGC 4 cut(s) 109, 144, 308, 957
Cfr10I RCCGGY 4 cut(s) 87, 109, 128, 169
Cfr13I GGNCC 5 cut(s) 34, 112, 177, 211, 472
CseI GACGC 1 cut(s) 602
Csp6I GTAC 2 cut(s) 119, 516
CspAI ACCGGT 1 cut(s) 128
CviAII CATG 8 cut(s) 12, 40, 97, 529, 643, 672, 887, 1030
CviQI GTAC 2 cut(s) 119, 516
DdeI CTNAG 3 cut(s) 302, 421, 480
DinI GGCGCC 1 cut(s) 108
DpnI GATC 3 cut(s) 287, 647, 850
DpnII GATC 3 cut(s) 285, 645, 848
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 397, 487
EarI CTCTTC 2 cut(s) 397, 487
EciI GGCGGA 1 cut(s) 326
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 528
Eco24I GRGCYC 1 cut(s) 78
Eco32I GATATC 1 cut(s) 640
Eco47I GGWCC 2 cut(s) 34, 472
Eco53kI GAGCTC 1 cut(s) 76
Eco57I CTGAAG 1 cut(s) 519
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 3 cut(s) 44, 897, 924
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 528
EcoT38I GRGCYC 1 cut(s) 78
EgeI GGCGCC 1 cut(s) 108
EheI GGCGCC 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 8 cut(s) 15, 43, 100, 532, 646, 675, 890, 1033
FalI AAGNNNNNCTT 2 cut(s) 1028, 1060
FaqI GGGAC 1 cut(s) 29
FatI CATG 8 cut(s) 11, 39, 96, 528, 642, 671, 886, 1029
FauI CCCGC 1 cut(s) 96
FblI GTMKAC 1 cut(s) 933
FokI GGATG 2 cut(s) 758, 826
FriOI GRGCYC 1 cut(s) 78
GlaI GCGC 4 cut(s) 108, 143, 307, 956
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 1 cut(s) 864
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 4 cut(s) 113, 179, 213, 521
HapII CCGG 8 cut(s) 88, 110, 129, 170, 180, 192, 323, 475
HgaI GACGC 1 cut(s) 602
HhaI GCGC 4 cut(s) 109, 144, 308, 957
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 8 cut(s) 15, 43, 100, 532, 646, 675, 890, 1033
Hin6I GCGC 4 cut(s) 107, 142, 306, 955
HinP1I GCGC 4 cut(s) 107, 142, 306, 955
HindIII AAGCTT 1 cut(s) 582
HinfI GANTC 3 cut(s) 262, 558, 836
HpaII CCGG 8 cut(s) 88, 110, 129, 170, 180, 192, 323, 475
HphI GGTGA 1 cut(s) 815
Hpy166II GTNNAC 2 cut(s) 875, 934
Hpy188I TCNGA 1 cut(s) 424
Hpy188III TCNNGA 5 cut(s) 248, 438, 643, 843, 1048
Hpy8I GTNNAC 2 cut(s) 875, 934
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 440
HpyCH4III ACNGT 3 cut(s) 511, 633, 986
HpyF10VI GCNNNNNNNGC 9 cut(s) 97, 100, 106, 163, 219, 325, 921, 961, 1032
HpyF3I CTNAG 3 cut(s) 302, 421, 480
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 8 cut(s) 15, 43, 100, 532, 646, 675, 890, 1033
HspAI GCGC 4 cut(s) 107, 142, 306, 955
KasI GGCGCC 1 cut(s) 106
KroI GCCGGC 1 cut(s) 109
KroNI GCCGGC 1 cut(s) 111
Kzo9I GATC 3 cut(s) 285, 645, 848
LmnI GCTCC 5 cut(s) 81, 141, 199, 227, 601
Lsp1109I GCAGC 5 cut(s) 178, 189, 231, 652, 902
LweI GCATC 2 cut(s) 856, 927
MaeIII GTNAC 3 cut(s) 83, 590, 1005
MalI GATC 3 cut(s) 287, 647, 850
MboI GATC 3 cut(s) 285, 645, 848
MboII GAAGA 4 cut(s) 414, 504, 640, 959
MflI RGATCY 1 cut(s) 848
MhlI GDGCHC 2 cut(s) 78, 877
MluCI AATT 5 cut(s) 243, 441, 523, 788, 860
Mly113I GGCGCC 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 410
MroNI GCCGGC 1 cut(s) 109
MseI TTAA 3 cut(s) 587, 768, 819
MslI CAYNNNNRTG 1 cut(s) 23
MspI CCGG 8 cut(s) 88, 110, 129, 170, 180, 192, 323, 475
MspR9I CCNGG 6 cut(s) 46, 181, 193, 476, 899, 926
MvaI CCWGG 3 cut(s) 46, 899, 926
MvnI CGCG 2 cut(s) 123, 308
MwoI GCNNNNNNNGC 9 cut(s) 97, 100, 106, 163, 219, 325, 921, 961, 1032
NaeI GCCGGC 1 cut(s) 111
NarI GGCGCC 1 cut(s) 107
NciI CCSGG 3 cut(s) 181, 193, 476
NcoI CCATGG 1 cut(s) 528
NdeII GATC 3 cut(s) 285, 645, 848
NgoMIV GCCGGC 1 cut(s) 109
NlaIII CATG 8 cut(s) 15, 43, 100, 532, 646, 675, 890, 1033
NlaIV GGNNCC 8 cut(s) 36, 108, 114, 127, 137, 327, 895, 988
NmuCI GTSAC 1 cut(s) 590
NspI RCATGY 2 cut(s) 675, 890
PagI TCATGA 1 cut(s) 642
PasI CCCWGGG 1 cut(s) 45
PciI ACATGT 1 cut(s) 671
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PdiI GCCGGC 1 cut(s) 111
PfeI GAWTC 3 cut(s) 262, 558, 836
PinAI ACCGGT 1 cut(s) 128
PluTI GGCGCC 1 cut(s) 110
PscI ACATGT 1 cut(s) 671
PshBI ATTAAT 1 cut(s) 768
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 3 cut(s) 44, 897, 924
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 3 cut(s) 44, 897, 924
PspN4I GGNNCC 8 cut(s) 36, 108, 114, 127, 137, 327, 895, 988
PspPI GGNCC 5 cut(s) 34, 112, 177, 211, 472
PstI CTGCAG 2 cut(s) 370, 797
PsuI RGATCY 1 cut(s) 848
RsaI GTAC 2 cut(s) 120, 517
RsaNI GTAC 2 cut(s) 119, 516
RseI CAYNNNNRTG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 78
SaqAI TTAA 3 cut(s) 587, 768, 819
Sau3AI GATC 3 cut(s) 285, 645, 848
Sau96I GGNCC 5 cut(s) 34, 112, 177, 211, 472
ScrFI CCNGG 6 cut(s) 46, 181, 193, 476, 899, 926
SduI GDGCHC 2 cut(s) 78, 877
SetI ASST 8 cut(s) 78, 204, 224, 338, 474, 586, 730, 776
SfaNI GCATC 2 cut(s) 856, 927
SfcI CTRYAG 3 cut(s) 366, 753, 793
SfoI GGCGCC 1 cut(s) 108
SinI GGWCC 2 cut(s) 34, 472
SmiMI CAYNNNNRTG 1 cut(s) 23
Sse9I AATT 5 cut(s) 243, 441, 523, 788, 860
SsiI CCGC 8 cut(s) 8, 66, 91, 100, 103, 123, 308, 311
SspDI GGCGCC 1 cut(s) 106
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 6 cut(s) 44, 179, 191, 474, 897, 924
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 3 cut(s) 511, 633, 986
TaqI TCGA 1 cut(s) 1041
TaqII GACCGA 1 cut(s) 679
TasI AATT 5 cut(s) 243, 441, 523, 788, 860
TauI GCSGC 6 cut(s) 11, 68, 94, 103, 126, 311
TfiI GAWTC 3 cut(s) 262, 558, 836
Tru1I TTAA 3 cut(s) 587, 768, 819
Tru9I TTAA 3 cut(s) 587, 768, 819
TscAI CASTG 2 cut(s) 543, 877
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 5 cut(s) 166, 202, 219, 665, 915
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 3 cut(s) 362, 474, 640
TspGWI ACGGA 2 cut(s) 71, 1077
TspRI CASTG 2 cut(s) 543, 877
VneI GTGCAC 1 cut(s) 873
VpaK11BI GGWCC 2 cut(s) 34, 472
VspI ATTAAT 1 cut(s) 768
XapI RAATTY 2 cut(s) 788, 860
XceI RCATGY 2 cut(s) 675, 890
XcmI CCANNNNNNNNNTGG 3 cut(s) 46, 535, 839
XmiI GTMKAC 1 cut(s) 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.