Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0018073

Physical Location & Seq

Genomic Coordinates Forward (+)

33319602 .. 33322211

Gene Structure 2610 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0231400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGGCACTTGCTACGTCATCAGCTCAAGAAGCTTCTTCTTCCAACGATTCGTCTTCTCGATTCAGTTATCACGTTTTCTTGAGTTTCAGAGGCGAGGAGACTCGCAAGACTTTCACTGACCACCTCTACACAGCCTTGACCAATGCAGGGTTTCGCACTTTCCGAGACGATGATGAGCTTCCCAGAGGAGAAGACATCATCAAACCAGAACTAGAGAGAGCCATCCAACAGTCAAAAAGTTCTGTCATTGTGTTTTCCAAAGACTACGCTTCTTCCGGATGGTGCCTTGACCAGCTTGTCATGATCCTTCAACGCAAGAAGACCTCATCCAACCATGTTGTCTTACCAGTCTTCTACGATATCGATCCATCTCACGTGAGGAAGCAAACAGGGAGTGTTGGAAAAGCATTTGCTAAACATCAGAAAAAGCAATCACCGGAGAAGTTGAATAGATGGAGGGAAGCACTTGCACAAGTTGCAGATCTAGCAGGAATGGTGCTCCAAAATGAAGCAGATGGGCATGAGGCAAAGTTTATCAAGAAAATTGTTAAAGTGATTGAAGACAAACTGTGTCGCGTGCCCTTAAGTGTTACACCTTACCTAGTTGGAATTCAATATCAGGTTGAAAACATTAACTTATGGGTACAAGATGGTTCATCTGATGTTGGCATATGGGGAATTTGTGGTATTGGTGGAATAGGAAAGACAACCATTGCACGAGTTGTTTACAATTCTAATTTTGCAAGATTCGAAGGGAGCAGTTTCATTGAAAATGTGAGAGAAATTTCAGAACAACCCAATGGCTTAGTTCGGATACAGAAACAACTCCTTGCGGATATTTTGAGTGGCAGAAAAGTGAAAATACAGAGTGTTAGTGAGGGGATATTGAAGATTAAGGATGTCATAGGATCTAAAAAAGTACTTGTTGTTCTAGACGATATTGATCGTATGGGCCAATTAGATGCAGTACAAAGTATGAGAGATTGTTTCTGTCCAGGAAGTAAAATTATCATAACAACTAGGCATGCAGGGTTGTTAGAGGCTCGTCGAGTTGATAAGGTGCAAATGGTTGAATTTCTAAACGATGTTCATTCACTGGAGCTCTTTAGTTGGCATTGTTTTCGACAGGACCATCCCATAGAAGGTTACATGGAACTTTCAGAAAGGGTAGTTAACCACTGTGGAGGACTTCCCTTAGCTCTTCAGACTCTGGCTTCTTCTCTTTTAGGGAAAAGTTTAGATGTATGGCAAAGTGCACTAAAGAAATTAAAGGCTATTTCAAACAGTGAAGTTTTGAGTAAACTAAGAATAAGCTATGATTCTTTACAAGATGACCATGACCAAAATTTGTTCCTCCATATTGCATGTTTCTTTATTGGAAAGGAGAAGAATGTCATTGTCAAAATACTAGATGGATGTGATTTCTACACCATTGTTGGCATTCAAAACCTCATTGACAGATGTTTGGTGTTTTGTGATGGTTATAACAAGATGAGGATGCATCAAATGATTAGAGACATGGGAAGAGAAATTGTTCGCCTAGAATCAAAGTATCCTGAGAAACGCAGTAGATTATGGCATCATAGAGATTCTTTCAATGTACTGAGAGAAAAAAATGGTTCAAAAAGAATTGAAGGCCTCGTCCTGAACATGCATATGTACCTCGAAGACAATCCTTCAAGAAATTCAGATGAGGTAGTGTTGGAAACAAATGCATTTACAAGTATGCGCAAACTAAGATTACTAGAATTTTGTGATGTACAACTGAATGGAAGTTATGAAGAGTTTCCTAAAGGATTAAGATGGTTGTGCTGGGCTGAATTCCCTTTAGATTCTCTTCCGAGTGATTTTCTTTTGGAGTACCTTGTTGTACTTGAAATGCCCAGTAGTAACTTGAGACAGGTGTGGAAGGGAATGAAGAACCAGCTTCCATCATTGAAGATCCTTGATCTCAGCCGTTCCATTTACCTGACTGAAGTTAGTGAGTTTTCATTAGTCCCCAATCTAGAAAAATTGATTCTTGAACATTGTCCAAGCTTGGTTGAAATCCATGAATCCATTGGAAACCTAGAGAGTCTTGTTTATTTGACTTTGAAAGATTGCAAAACTATTAGCAAGCTTCCAGAGAGTTTTTCTAAGCTTAAATCACTGGAGACGGTTGTTATATCTGGTTGCTCAAATCTGAAAGAGTTTCCAGTGCAGATGAGTAAGATGGAATCTCTGAAAGTGCTTGAAATAGATGAAGTTCCCATAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031090 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

752

Amino Acids

85.5

Weight (kDa)

6.84

Isoelectric Point (pI)

46.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	23 - 193	2.7e-50	TIR domain
TIR_2	PF13676	25 - 123	1.3e-14	TIR domain
NB-ARC	PF00931	206 - 376	1.3e-17	NB-ARC domain
WHD_ROQ1	PF23282	445 - 515	1.7e-19	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	671 - 748	4.9e-08	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0018073)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g13390
rosa_chinensis	RchiOBHm_Chr1g0352711
rosa_laevigata	RLG00000028317
rosa_multiflora	Rmu_sc0006445.1_g000001
rosa_roxburghii	Rroxscaffold_4G00302420
rosa_rugosa	Rorug01G0231300 Rorug01G0231400
rosa_samantha	Rh1AG244400 Rh1BG215200 Rh1CG228200 Rh1DG241200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1485
AasI	GACNNNNNNGTC	1 cut(s)	296
Acc16I	TGCGCA	1 cut(s)	1730
AccB1I	GGYRCC	1 cut(s)	282
AccII	CGCG	1 cut(s)	576
AccIII	TCCGGA	1 cut(s)	275
AciI	CCGC	1 cut(s)	833
AclWI	GGATC	4 cut(s)	298, 359, 916, 1936
AcsI	RAATTY	8 cut(s)	609, 678, 783, 1073, 1345, 1684, 1748, 1820
AcuI	CTGAAG	2 cut(s)	1187, 1995
AcvI	CACGTG	1 cut(s)	376
AfaI	GTAC	8 cut(s)	645, 921, 969, 1602, 1661, 1761, 1862, 1872
AfiI	CCNNNNNNNGG	2 cut(s)	147, 1142
AflII	CTTAAG	1 cut(s)	583
AjnI	CCWGG	1 cut(s)	994
Alw21I	GWGCWC	3 cut(s)	501, 1104, 1258
Alw26I	GTCTC	5 cut(s)	92, 159, 1509, 1891, 2147
Alw44I	GTGCAC	1 cut(s)	1254
AlwI	GGATC	4 cut(s)	298, 359, 916, 1936
AlwNI	CAGNNNCTG	1 cut(s)	1210
Aor13HI	TCCGGA	1 cut(s)	275
AoxI	GGCC	2 cut(s)	952, 1636
ApaLI	GTGCAC	1 cut(s)	1254
ApoI	RAATTY	8 cut(s)	609, 678, 783, 1073, 1345, 1684, 1748, 1820
Asp700I	GAANNNNTTC	2 cut(s)	1533, 1785
AspLEI	GCGC	1 cut(s)	1731
AspS9I	GGNCC	2 cut(s)	952, 1129
AsuHPI	GGTGA	1 cut(s)	426
AsuII	TTCGAA	1 cut(s)	750
AvaII	GGWCC	1 cut(s)	1129
BaeGI	GKGCMC	2 cut(s)	582, 1258
BanI	GGYRCC	1 cut(s)	282
BanII	GRGCYC	1 cut(s)	1104
BauI	CACGAG	1 cut(s)	717
BbrPI	CACGTG	1 cut(s)	376
BbsI	GAAGAC	6 cut(s)	45, 198, 326, 343, 567, 1674
Bbv12I	GWGCWC	3 cut(s)	501, 1104, 1258
BceAI	ACGGC	1 cut(s)	1941
BcgI	CGANNNNNNTGC	2 cut(s)	1103, 1137
BciT130I	CCWGG	1 cut(s)	996
BciVI	GTATCC	2 cut(s)	807, 1563
BcoDI	GTCTC	5 cut(s)	92, 159, 1509, 1891, 2147
BfrI	CTTAAG	1 cut(s)	583
BfuI	GTATCC	2 cut(s)	807, 1563
BglII	AGATCT	1 cut(s)	481
BmcAI	AGTACT	1 cut(s)	921
Bme1390I	CCNGG	1 cut(s)	996
Bme18I	GGWCC	1 cut(s)	1129
BmgT120I	GGNCC	2 cut(s)	952, 1129
BmiI	GGNNCC	1 cut(s)	284
BmrFI	CCNGG	1 cut(s)	996
BmrI	ACTGGG	1 cut(s)	1878
BmsI	GCATC	4 cut(s)	952, 1488, 1510, 1588
BmuI	ACTGGG	1 cut(s)	1878
BpiI	GAAGAC	6 cut(s)	45, 198, 326, 343, 567, 1674
BpmI	CTGGAG	2 cut(s)	1118, 2171
Bpu10I	CCTNAGC	1 cut(s)	1195
Bpu14I	TTCGAA	1 cut(s)	750
BpuEI	CTTGAG	3 cut(s)	9, 100, 1915
Bsa29I	ATCGAT	1 cut(s)	363
BsaAI	YACGTR	1 cut(s)	376
BsaBI	GATNNNNATC	2 cut(s)	363, 942
BsaWI	WCCGGW	2 cut(s)	275, 436
Bsc4I	CCNNNNNNNGG	2 cut(s)	147, 1142
Bse1I	ACTGG	5 cut(s)	347, 1101, 1884, 2154, 2195
Bse3DI	GCAATG	1 cut(s)	711
Bse8I	GATNNNNATC	2 cut(s)	363, 942
BseAI	TCCGGA	1 cut(s)	275
BseBI	CCWGG	1 cut(s)	996
BseCI	ATCGAT	1 cut(s)	363
BseGI	GGATG	7 cut(s)	222, 284, 326, 904, 1132, 1421, 1503
BseJI	GATNNNNATC	2 cut(s)	363, 942
BseLI	CCNNNNNNNGG	2 cut(s)	147, 1142
BseMI	GCAATG	1 cut(s)	711
BseMII	CTCAG	3 cut(s)	1548, 1595, 1966
BseNI	ACTGG	5 cut(s)	347, 1101, 1884, 2154, 2195
BseRI	GAGGAG	2 cut(s)	110, 201
BseSI	GKGCMC	2 cut(s)	582, 1258
BseYI	CCCAGC	1 cut(s)	1812
BsgI	GTGCAG	1 cut(s)	2219
Bsh1236I	CGCG	1 cut(s)	576
BshFI	GGCC	2 cut(s)	954, 1638
BshNI	GGYRCC	1 cut(s)	282
BshVI	ATCGAT	1 cut(s)	363
BsiHKAI	GWGCWC	3 cut(s)	501, 1104, 1258
BsiSI	CCGG	2 cut(s)	276, 437
BslFI	GGGAC	1 cut(s)	1982
BslI	CCNNNNNNNGG	2 cut(s)	147, 1142
BsmAI	GTCTC	5 cut(s)	92, 159, 1509, 1891, 2147
BsmBI	CGTCTC	2 cut(s)	159, 2147
BsmFI	GGGAC	1 cut(s)	1982
BsmI	GAATGC	1 cut(s)	1440
BsnI	GGCC	2 cut(s)	954, 1638
Bsp119I	TTCGAA	1 cut(s)	750
Bsp1286I	GDGCHC	4 cut(s)	501, 582, 1104, 1258
Bsp13I	TCCGGA	1 cut(s)	275
Bsp1407I	TGTACA	1 cut(s)	1759
Bsp143I	GATC	7 cut(s)	303, 364, 481, 908, 943, 1941, 1948
BspACI	CCGC	1 cut(s)	833
BspANI	GGCC	2 cut(s)	954, 1638
BspCNI	CTCAG	3 cut(s)	1549, 1596, 1965
BspDI	ATCGAT	1 cut(s)	363
BspEI	TCCGGA	1 cut(s)	275
BspFNI	CGCG	1 cut(s)	576
BspHI	TCATGA	1 cut(s)	300
BspLI	GGNNCC	1 cut(s)	284
BspPI	GGATC	4 cut(s)	298, 359, 916, 1936
BspQI	GCTCTTC	1 cut(s)	1206
BspT104I	TTCGAA	1 cut(s)	750
BspT107I	GGYRCC	1 cut(s)	282
BspTI	CTTAAG	1 cut(s)	583
BsrDI	GCAATG	1 cut(s)	711
BsrGI	TGTACA	1 cut(s)	1759
BsrI	ACTGG	5 cut(s)	347, 1101, 1884, 2154, 2195
BssMI	GATC	7 cut(s)	303, 364, 481, 908, 943, 1941, 1948
BssSI	CACGAG	1 cut(s)	717
Bst2BI	CACGAG	1 cut(s)	717
Bst2UI	CCWGG	1 cut(s)	996
Bst4CI	ACNGT	5 cut(s)	231, 570, 1181, 1286, 2158
Bst6I	CTCTTC	4 cut(s)	1206, 1519, 1776, 1842
BstAFI	CTTAAG	1 cut(s)	583
BstAPI	GCANNNNNTGC	1 cut(s)	476
BstAUI	TGTACA	1 cut(s)	1759
BstBAI	YACGTR	1 cut(s)	376
BstBI	TTCGAA	1 cut(s)	750
BstC8I	GCNNGC	3 cut(s)	578, 1026, 2117
BstDEI	CTNAG	8 cut(s)	805, 1195, 1304, 1557, 1604, 1736, 1952, 2136
BstF5I	GGATG	7 cut(s)	222, 284, 326, 904, 1132, 1421, 1503
BstFNI	CGCG	1 cut(s)	576
BstHHI	GCGC	1 cut(s)	1731
BstKTI	GATC	7 cut(s)	306, 367, 484, 911, 946, 1944, 1951
BstMAI	GTCTC	5 cut(s)	92, 159, 1509, 1891, 2147
BstMBI	GATC	7 cut(s)	303, 364, 481, 908, 943, 1941, 1948
BstMWI	GCNNNNNNNGC	3 cut(s)	29, 476, 485
BstNI	CCWGG	1 cut(s)	996
BstNSI	RCATGY	3 cut(s)	1028, 1368, 1654
BstSCI	CCNGG	1 cut(s)	994
BstSLI	GKGCMC	2 cut(s)	582, 1258
BstUI	CGCG	1 cut(s)	576
BstV2I	GAAGAC	6 cut(s)	45, 198, 326, 343, 567, 1674
BstX2I	RGATCY	3 cut(s)	481, 908, 1941
BstYI	RGATCY	3 cut(s)	481, 908, 1941
Bsu15I	ATCGAT	1 cut(s)	363
BsuI	GTATCC	2 cut(s)	807, 1563
BsuRI	GGCC	2 cut(s)	954, 1638
BsuTUI	ATCGAT	1 cut(s)	363
BtsCI	GGATG	7 cut(s)	222, 284, 326, 904, 1132, 1421, 1503
BtsIMutI	CAGTG	6 cut(s)	114, 1094, 1177, 1291, 2147, 2202
Cac8I	GCNNGC	3 cut(s)	578, 1026, 2117
CaiI	CAGNNNCTG	1 cut(s)	1210
CciI	TCATGA	1 cut(s)	300
CfoI	GCGC	1 cut(s)	1731
Cfr13I	GGNCC	2 cut(s)	952, 1129
ClaI	ATCGAT	1 cut(s)	363
Csp6I	GTAC	8 cut(s)	644, 920, 968, 1601, 1660, 1760, 1861, 1871
CviQI	GTAC	8 cut(s)	644, 920, 968, 1601, 1660, 1760, 1861, 1871
DdeI	CTNAG	8 cut(s)	805, 1195, 1304, 1557, 1604, 1736, 1952, 2136
DpnI	GATC	7 cut(s)	305, 366, 483, 910, 945, 1943, 1950
DpnII	GATC	7 cut(s)	303, 364, 481, 908, 943, 1941, 1948
DrdI	GACNNNNNNGTC	1 cut(s)	296
DseDI	GACNNNNNNGTC	1 cut(s)	296
Eam1104I	CTCTTC	4 cut(s)	1206, 1519, 1776, 1842
EarI	CTCTTC	4 cut(s)	1206, 1519, 1776, 1842
Ecl136II	GAGCTC	1 cut(s)	1102
Eco147I	AGGCCT	1 cut(s)	1638
Eco24I	GRGCYC	1 cut(s)	1104
Eco32I	GATATC	1 cut(s)	361
Eco47I	GGWCC	1 cut(s)	1129
Eco53kI	GAGCTC	1 cut(s)	1102
Eco57I	CTGAAG	2 cut(s)	1187, 1995
Eco72I	CACGTG	1 cut(s)	376
EcoICRI	GAGCTC	1 cut(s)	1102
EcoRI	GAATTC	2 cut(s)	609, 1820
EcoRII	CCWGG	1 cut(s)	994
EcoRV	GATATC	1 cut(s)	361
EcoT22I	ATGCAT	3 cut(s)	1503, 1656, 1717
EcoT38I	GRGCYC	1 cut(s)	1104
Esp3I	CGTCTC	2 cut(s)	159, 2147
FaqI	GGGAC	1 cut(s)	1982
FauNDI	CATATG	2 cut(s)	671, 1656
FokI	GGATG	7 cut(s)	209, 291, 313, 911, 1119, 1428, 1510
FriOI	GRGCYC	1 cut(s)	1104
FspI	TGCGCA	1 cut(s)	1730
GlaI	GCGC	1 cut(s)	1730
GsaI	CCCAGC	1 cut(s)	1816
GsuI	CTGGAG	2 cut(s)	1118, 2171
HaeIII	GGCC	2 cut(s)	954, 1638
HapII	CCGG	2 cut(s)	276, 437
HhaI	GCGC	1 cut(s)	1731
Hin6I	GCGC	1 cut(s)	1729
HinP1I	GCGC	1 cut(s)	1729
HincII	GTYRAC	1 cut(s)	1174
HindII	GTYRAC	1 cut(s)	1174
HindIII	AAGCTT	4 cut(s)	30, 2035, 2117, 2138
HpaI	GTTAAC	1 cut(s)	1174
HpaII	CCGG	2 cut(s)	276, 437
HphI	GGTGA	1 cut(s)	426
Hpy166II	GTNNAC	4 cut(s)	727, 1174, 1256, 1301
Hpy8I	GTNNAC	4 cut(s)	727, 1174, 1256, 1301
Hpy99I	CGWCG	1 cut(s)	1050
HpyAV	CCTTC	6 cut(s)	317, 746, 1136, 1628, 1686, 1903
HpyCH4III	ACNGT	5 cut(s)	231, 570, 1181, 1286, 2158
HpyCH4IV	ACGT	3 cut(s)	14, 72, 375
HpyF10VI	GCNNNNNNNGC	3 cut(s)	29, 476, 485
HpyF3I	CTNAG	8 cut(s)	805, 1195, 1304, 1557, 1604, 1736, 1952, 2136
HpySE526I	ACGT	3 cut(s)	14, 72, 375
HspAI	GCGC	1 cut(s)	1729
Kpn2I	TCCGGA	1 cut(s)	275
KspAI	GTTAAC	1 cut(s)	1174
Kzo9I	GATC	7 cut(s)	303, 364, 481, 908, 943, 1941, 1948
LguI	GCTCTTC	1 cut(s)	1206
LmnI	GCTCC	3 cut(s)	504, 756, 1099
LweI	GCATC	4 cut(s)	952, 1488, 1510, 1588
MaeII	ACGT	3 cut(s)	14, 72, 375
MaeIII	GTNAC	3 cut(s)	589, 1145, 1889
MalI	GATC	7 cut(s)	305, 366, 483, 910, 945, 1943, 1950
MboI	GATC	7 cut(s)	303, 364, 481, 908, 943, 1941, 1948
MflI	RGATCY	3 cut(s)	481, 908, 1941
MhlI	GDGCHC	4 cut(s)	501, 582, 1104, 1258
MlyI	GAGTC	3 cut(s)	94, 1201, 2083
MmeI	TCCRAC	6 cut(s)	66, 250, 354, 379, 586, 1683
Mph1103I	ATGCAT	3 cut(s)	1503, 1656, 1717
MroI	TCCGGA	1 cut(s)	275
MroXI	GAANNNNTTC	2 cut(s)	1533, 1785
MseI	TTAA	8 cut(s)	549, 584, 633, 894, 1173, 1268, 1799, 2142
MslI	CAYNNNNRTG	1 cut(s)	1655
MspCI	CTTAAG	1 cut(s)	583
MspI	CCGG	2 cut(s)	276, 437
MspR9I	CCNGG	1 cut(s)	996
Mva1269I	GAATGC	1 cut(s)	1440
MvaI	CCWGG	1 cut(s)	996
MvnI	CGCG	1 cut(s)	576
MwoI	GCNNNNNNNGC	3 cut(s)	29, 476, 485
NdeI	CATATG	2 cut(s)	671, 1656
NdeII	GATC	7 cut(s)	303, 364, 481, 908, 943, 1941, 1948
NlaIV	GGNNCC	1 cut(s)	284
NsbI	TGCGCA	1 cut(s)	1730
NsiI	ATGCAT	3 cut(s)	1503, 1656, 1717
NspI	RCATGY	3 cut(s)	1028, 1368, 1654
NspV	TTCGAA	1 cut(s)	750
PaeI	GCATGC	1 cut(s)	1028
PagI	TCATGA	1 cut(s)	300
PceI	AGGCCT	1 cut(s)	1638
PciSI	GCTCTTC	1 cut(s)	1206
PcsI	WCGNNNNNNNCGW	1 cut(s)	160
PctI	GAATGC	1 cut(s)	1440
PdmI	GAANNNNTTC	2 cut(s)	1533, 1785
PfoI	TCCNGGA	1 cut(s)	994
PleI	GAGTC	3 cut(s)	94, 1201, 2082
PmaCI	CACGTG	1 cut(s)	376
PmlI	CACGTG	1 cut(s)	376
PpsI	GAGTC	3 cut(s)	94, 1201, 2082
Ppu21I	YACGTR	1 cut(s)	376
PsiI	TTATAA	1 cut(s)	1485
Psp124BI	GAGCTC	1 cut(s)	1104
Psp6I	CCWGG	1 cut(s)	994
PspCI	CACGTG	1 cut(s)	376
PspFI	CCCAGC	1 cut(s)	1812
PspGI	CCWGG	1 cut(s)	994
PspN4I	GGNNCC	1 cut(s)	284
PspPI	GGNCC	2 cut(s)	952, 1129
PsrI	GAACNNNNNNTAC	2 cut(s)	912, 944
PstNI	CAGNNNCTG	1 cut(s)	1210
PsuI	RGATCY	3 cut(s)	481, 908, 1941
RsaI	GTAC	8 cut(s)	645, 921, 969, 1602, 1661, 1761, 1862, 1872
RsaNI	GTAC	8 cut(s)	644, 920, 968, 1601, 1660, 1760, 1861, 1871
RseI	CAYNNNNRTG	1 cut(s)	1655
SacI	GAGCTC	1 cut(s)	1104
SapI	GCTCTTC	1 cut(s)	1206
SaqAI	TTAA	8 cut(s)	549, 584, 633, 894, 1173, 1268, 1799, 2142
Sau3AI	GATC	7 cut(s)	303, 364, 481, 908, 943, 1941, 1948
Sau96I	GGNCC	2 cut(s)	952, 1129
ScaI	AGTACT	1 cut(s)	921
SchI	GAGTC	3 cut(s)	94, 1201, 2083
ScrFI	CCNGG	1 cut(s)	996
SduI	GDGCHC	4 cut(s)	501, 582, 1104, 1258
SfaNI	GCATC	4 cut(s)	952, 1488, 1510, 1588
SfuI	TTCGAA	1 cut(s)	750
SinI	GGWCC	1 cut(s)	1129
SmiMI	CAYNNNNRTG	1 cut(s)	1655
SmlI	CTYRAG	4 cut(s)	24, 79, 583, 1894
SmoI	CTYRAG	4 cut(s)	24, 79, 583, 1894
SphI	GCATGC	1 cut(s)	1028
SseBI	AGGCCT	1 cut(s)	1638
SsiI	CCGC	1 cut(s)	833
SstI	GAGCTC	1 cut(s)	1104
StuI	AGGCCT	1 cut(s)	1638
StyD4I	CCNGG	1 cut(s)	994
TaaI	ACNGT	5 cut(s)	231, 570, 1181, 1286, 2158
TaiI	ACGT	3 cut(s)	17, 75, 378
TaqI	TCGA	6 cut(s)	58, 363, 750, 1048, 1123, 1665
TatI	WGTACW	5 cut(s)	919, 967, 1600, 1759, 1870
Tru1I	TTAA	8 cut(s)	549, 584, 633, 894, 1173, 1268, 1799, 2142
Tru9I	TTAA	8 cut(s)	549, 584, 633, 894, 1173, 1268, 1799, 2142
TscAI	CASTG	6 cut(s)	121, 1101, 1184, 1291, 2154, 2202
TspDTI	ATGAA	9 cut(s)	522, 645, 754, 1079, 1794, 1931, 1980, 2067, 2256
TspRI	CASTG	6 cut(s)	121, 1101, 1184, 1291, 2154, 2202
Vha464I	CTTAAG	1 cut(s)	583
VneI	GTGCAC	1 cut(s)	1254
VpaK11BI	GGWCC	1 cut(s)	1129
XapI	RAATTY	8 cut(s)	609, 678, 783, 1073, 1345, 1684, 1748, 1820
XbaI	TCTAGA	2 cut(s)	931, 2005
XceI	RCATGY	3 cut(s)	1028, 1368, 1654
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2057
XmnI	GAANNNNTTC	2 cut(s)	1533, 1785
ZrmI	AGTACT	1 cut(s)	921
Zsp2I	ATGCAT	3 cut(s)	1503, 1656, 1717

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.