Rorug01G0323700

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0019788
Physical Location & Seq
Reverse (-)
44263817 .. 44265747
1931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0323700.1

Sequence Viewer

Length: 1089 bp
ATGGCCTCTTCACTGGAAACAAAACCCCAAGCCATAGTTCTTGACGATGAAAGAAAGCAAGAAGAAGAAAGCTTTCATTATGCTGTGCAGCTGGTGGTTTCATCTGCGCTGCCCATGTCCATGCAATCAGCCATTGAGCTCGGACTTTTTGATATCATAGCCAGAGCAGGTTCGGGTGTGGGGCTCTCTGCATCCCAGATTGCTGCCCAGATTGGCACCCAGAATCCTGAGGCAGCCTTTATGCTCGATCGAATCCTTAGGCTCCTCGCCACTCACTCTGTACTTGGTTGTTCTCTGGTTGATGGCCAAAGGCTCTACAGGCTCTCCGCGGTGTCCAAGCACTTTGTGACTGAAGATGGCGTTTCGTTGGGCTTCGTCATGGCATTGTTCCAAGACAAGGTCTTCATAAACAGTTGGTCACAACTGAAAGACGCAGTTATTGAAGGTGGAATTCCATTTGAGAGGTTCCATGGCATGCCTACCTTTGAGTATTCAGGTTCGGACCAGAGGTTTAATCAGGTCTTCAACACAGCAATGTTCAACCACACCACTCTTGTTGTGAAGCGGATTCTTGATCTCTACAGCGGTTTTGAGCACCTTGGGGAGCTTGTTGATGTTGGTGGTGGTTTGGGAGTAGCTCTCAGTCTAATCACTTCCAGATACCCTCATATTAAGGGAATCAATTATGATTTGCCGCATGTTATAAAATATGCTCACCCTTCTCCTGGTGTCGAACATGTTGGAGGAGACATGTTTGCCCATGTTCCAAGTGGAGATGCCATTTTCTTAAAGGGCATACTTCACGATTGGACGGATGAGGACTGCATAAAGTTGTTGAAGAATTGTTACAGTGCTACTCCAGACGATGGAAAAGTAATAGTTTTGGAGGCAGTTCTTCCAGTTTTGCCGGAAACAAGCACTACTGAGAAGATCACCACCCAACTGGATGTGCTTATGATGACTCAATGCCCAGGAGGGAAGGAGAGGACCCAACAAGAATTCATGGACTTGGCAACTGGTGCTGGATTCAGTGGCATCAAATATGAATGTTTCGTCGCTAATATATGGGTCATGGAGTTTATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

362

Amino Acids

39.73

Weight (kDa)

5.13

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 30 - 117 3.3e-18 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 139 - 344 1.9e-64 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019788)

Species Orthologous Gene IDs
malus_domestica MD11G1084100.v1.1
rosa_multiflora Rmu_sc0002093.1_g000030
rosa_rugosa Rorug01G0323700
rosa_samantha Rh1AG332400 Rh1BG293300 Rh1CG310200 Rh1DG325600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
Acc36I ACCTGC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 215
AccB7I CCANNNNNTGG 1 cut(s) 866
AccII CGCG 1 cut(s) 329
AciI CCGC 5 cut(s) 327, 329, 565, 585, 695
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 2 cut(s) 450, 998
AcuI CTGAAG 1 cut(s) 372
AdeI CACNNNGTG 1 cut(s) 346
AfaI GTAC 1 cut(s) 282
AfiI CCNNNNNNNGG 3 cut(s) 397, 725, 866
AflIII ACRYGT 2 cut(s) 736, 750
AgsI TTSAA 4 cut(s) 443, 526, 541, 838
AjnI CCWGG 2 cut(s) 724, 970
AjuI GAANNNNNNNTTGG 2 cut(s) 21, 53
AluBI AGCT 5 cut(s) 72, 91, 139, 607, 638
AluI AGCT 5 cut(s) 72, 91, 139, 607, 638
Alw21I GWGCWC 2 cut(s) 141, 597
Alw26I GTCTC 1 cut(s) 741
AoxI GGCC 2 cut(s) 3, 304
ApeKI GCWGC 4 cut(s) 88, 109, 203, 233
ApoI RAATTY 2 cut(s) 450, 998
Asp700I GAANNNNTTC 1 cut(s) 72
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 2 cut(s) 502, 987
AsuHPI GGTGA 2 cut(s) 707, 925
AvaII GGWCC 2 cut(s) 502, 987
AxyI CCTNAGG 2 cut(s) 228, 257
BalI TGGCCA 1 cut(s) 306
BanI GGYRCC 1 cut(s) 215
BanII GRGCYC 2 cut(s) 141, 186
BarI GAAGNNNNNNTAC 2 cut(s) 830, 862
BbsI GAAGAC 2 cut(s) 394, 514
Bbv12I GWGCWC 2 cut(s) 141, 597
BbvI GCAGC 4 cut(s) 96, 100, 190, 245
BccI CCATC 3 cut(s) 296, 350, 860
BciT130I CCWGG 2 cut(s) 726, 972
BcoDI GTCTC 1 cut(s) 741
BfmI CTRYAG 2 cut(s) 316, 580
BfuAI ACCTGC 1 cut(s) 158
BisI GCNGC 5 cut(s) 89, 110, 204, 234, 695
BlsI GCNGC 5 cut(s) 90, 111, 205, 235, 696
Bme1390I CCNGG 2 cut(s) 726, 972
Bme18I GGWCC 2 cut(s) 502, 987
BmgT120I GGNCC 2 cut(s) 502, 987
BmiI GGNNCC 4 cut(s) 217, 263, 467, 989
BmrFI CCNGG 2 cut(s) 726, 972
BmsI GCATC 3 cut(s) 200, 766, 1044
BpiI GAAGAC 2 cut(s) 394, 514
BplI GAGNNNNNCTC 2 cut(s) 624, 656
BpmI CTGGAG 1 cut(s) 843
BsaJI CCNNGG 4 cut(s) 327, 469, 598, 970
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 3 cut(s) 397, 725, 866
Bse1I ACTGG 4 cut(s) 18, 899, 948, 1021
Bse21I CCTNAGG 2 cut(s) 228, 257
Bse3DI GCAATG 1 cut(s) 540
BseBI CCWGG 2 cut(s) 726, 972
BseDI CCNNGG 4 cut(s) 327, 469, 598, 970
BseGI GGATG 3 cut(s) 191, 820, 952
BseLI CCNNNNNNNGG 3 cut(s) 397, 725, 866
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 3 cut(s) 219, 655, 915
BseNI ACTGG 4 cut(s) 18, 899, 948, 1021
BseRI GAGGAG 2 cut(s) 254, 759
BseXI GCAGC 4 cut(s) 96, 100, 190, 245
BsgI GTGCAG 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 329
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 2 cut(s) 5, 306
BshNI GGYRCC 1 cut(s) 215
BsiEI CGRYCG 1 cut(s) 250
BsiHKAI GWGCWC 2 cut(s) 141, 597
BsiSI CCGG 1 cut(s) 908
BslI CCNNNNNNNGG 3 cut(s) 397, 725, 866
BsmAI GTCTC 1 cut(s) 741
BsnI GGCC 2 cut(s) 5, 306
Bsp1286I GDGCHC 3 cut(s) 141, 186, 597
Bsp143I GATC 3 cut(s) 247, 574, 930
Bsp19I CCATGG 1 cut(s) 469
BspACI CCGC 5 cut(s) 327, 329, 565, 585, 695
BspANI GGCC 2 cut(s) 5, 306
BspCNI CTCAG 3 cut(s) 220, 654, 916
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 4 cut(s) 217, 263, 467, 989
BspMI ACCTGC 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 4 cut(s) 18, 899, 948, 1021
BssECI CCNNGG 4 cut(s) 327, 469, 598, 970
BssMI GATC 3 cut(s) 247, 574, 930
BssT1I CCWWGG 2 cut(s) 469, 598
Bst2UI CCWGG 2 cut(s) 726, 972
Bst4CI ACNGT 2 cut(s) 413, 851
Bst6I CTCTTC 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 1019
BstC8I GCNNGC 1 cut(s) 476
BstDEI CTNAG 4 cut(s) 228, 257, 641, 924
BstDSI CCRYGG 2 cut(s) 327, 469
BstF5I GGATG 3 cut(s) 191, 820, 952
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 3 cut(s) 250, 577, 933
BstMAI GTCTC 1 cut(s) 741
BstMBI GATC 3 cut(s) 247, 574, 930
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 2 cut(s) 319, 1019
BstNI CCWGG 2 cut(s) 726, 972
BstNSI RCATGY 4 cut(s) 478, 701, 740, 754
BstSCI CCNGG 2 cut(s) 724, 970
BstSFI CTRYAG 2 cut(s) 316, 580
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 4 cut(s) 96, 100, 190, 245
BstV2I GAAGAC 2 cut(s) 394, 514
BstXI CCANNNNNNTGG 1 cut(s) 943
Bsu36I CCTNAGG 2 cut(s) 228, 257
BsuRI GGCC 2 cut(s) 5, 306
BtgI CCRYGG 2 cut(s) 327, 469
BtsCI GGATG 3 cut(s) 191, 820, 952
BtsIMutI CAGTG 3 cut(s) 11, 856, 1036
BveI ACCTGC 1 cut(s) 158
Cac8I GCNNGC 1 cut(s) 476
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 502, 987
Cfr42I CCGCGG 1 cut(s) 330
CseI GACGC 1 cut(s) 440
Csp6I GTAC 1 cut(s) 281
CspCI CAANNNNNGTGG 2 cut(s) 538, 573
CviQI GTAC 1 cut(s) 281
DdeI CTNAG 4 cut(s) 228, 257, 641, 924
DpnI GATC 3 cut(s) 249, 576, 932
DpnII GATC 3 cut(s) 247, 574, 930
DraIII CACNNNGTG 1 cut(s) 346
EaeI YGGCCR 1 cut(s) 304
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 469, 598
Eco24I GRGCYC 2 cut(s) 141, 186
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 2 cut(s) 502, 987
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 372
Eco81I CCTNAGG 2 cut(s) 228, 257
EcoICRI GAGCTC 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 987
EcoRI GAATTC 2 cut(s) 450, 998
EcoRII CCWGG 2 cut(s) 724, 970
EcoRV GATATC 1 cut(s) 154
EcoT14I CCWWGG 2 cut(s) 469, 598
EcoT38I GRGCYC 2 cut(s) 141, 186
ErhI CCWWGG 2 cut(s) 469, 598
Fnu4HI GCNGC 5 cut(s) 89, 110, 204, 234, 695
FokI GGATG 3 cut(s) 178, 827, 959
FriOI GRGCYC 2 cut(s) 141, 186
Fsp4HI GCNGC 5 cut(s) 89, 110, 204, 234, 695
GlaI GCGC 1 cut(s) 108
GluI GCNGC 5 cut(s) 89, 110, 204, 234, 695
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 2 cut(s) 5, 306
HapII CCGG 1 cut(s) 908
HgaI GACGC 1 cut(s) 440
HhaI GCGC 1 cut(s) 109
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HindIII AAGCTT 1 cut(s) 70
HinfI GANTC 6 cut(s) 223, 252, 568, 678, 961, 1026
HpaII CCGG 1 cut(s) 908
HphI GGTGA 2 cut(s) 707, 925
Hpy188I TCNGA 2 cut(s) 143, 502
Hpy188III TCNNGA 6 cut(s) 41, 227, 572, 657, 803, 860
Hpy99I CGWCG 1 cut(s) 1058
HpyAV CCTTC 3 cut(s) 437, 729, 973
HpyCH4III ACNGT 2 cut(s) 413, 851
HpyCH4V TGCA 4 cut(s) 88, 124, 191, 825
HpyF10VI GCNNNNNNNGC 2 cut(s) 319, 1019
HpyF3I CTNAG 4 cut(s) 228, 257, 641, 924
HspAI GCGC 1 cut(s) 107
KspI CCGCGG 1 cut(s) 330
Kzo9I GATC 3 cut(s) 247, 574, 930
LmnI GCTCC 2 cut(s) 267, 604
Lsp1109I GCAGC 4 cut(s) 96, 100, 190, 245
LweI GCATC 3 cut(s) 200, 766, 1044
MaeIII GTNAC 3 cut(s) 346, 417, 845
MalI GATC 3 cut(s) 249, 576, 932
MboI GATC 3 cut(s) 247, 574, 930
MboII GAAGA 8 cut(s) 74, 77, 365, 394, 514, 850, 887, 940
MhlI GDGCHC 3 cut(s) 141, 186, 597
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 4 cut(s) 450, 682, 841, 998
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 1 cut(s) 955
MmeI TCCRAC 1 cut(s) 721
Mox20I TGGCCA 1 cut(s) 306
MroXI GAANNNNTTC 1 cut(s) 72
MscI TGGCCA 1 cut(s) 306
MseI TTAA 4 cut(s) 513, 672, 788, 1083
MslI CAYNNNNRTG 2 cut(s) 119, 533
Msp20I TGGCCA 1 cut(s) 306
MspA1I CMGCKG 3 cut(s) 91, 329, 585
MspI CCGG 1 cut(s) 908
MspR9I CCNGG 2 cut(s) 726, 972
MvaI CCWGG 2 cut(s) 726, 972
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 2 cut(s) 319, 1019
NcoI CCATGG 1 cut(s) 469
NdeII GATC 3 cut(s) 247, 574, 930
NlaIV GGNNCC 4 cut(s) 217, 263, 467, 989
NmuCI GTSAC 2 cut(s) 346, 417
NspI RCATGY 4 cut(s) 478, 701, 740, 754
PaeI GCATGC 1 cut(s) 478
PciI ACATGT 2 cut(s) 736, 750
PdmI GAANNNNTTC 1 cut(s) 72
PfeI GAWTC 5 cut(s) 223, 252, 568, 678, 1026
PflFI GACNNNGTC 1 cut(s) 398
PflMI CCANNNNNTGG 1 cut(s) 866
PkrI GCNGC 5 cut(s) 90, 111, 205, 235, 696
Ple19I CGATCG 1 cut(s) 250
PleI GAGTC 1 cut(s) 955
PpsI GAGTC 1 cut(s) 955
PpuMI RGGWCCY 1 cut(s) 987
PscI ACATGT 2 cut(s) 736, 750
PsiI TTATAA 1 cut(s) 704
Psp124BI GAGCTC 1 cut(s) 141
Psp5II RGGWCCY 1 cut(s) 987
Psp6I CCWGG 2 cut(s) 724, 970
PspGI CCWGG 2 cut(s) 724, 970
PspN4I GGNNCC 4 cut(s) 217, 263, 467, 989
PspPI GGNCC 2 cut(s) 502, 987
PspPPI RGGWCCY 1 cut(s) 987
PsyI GACNNNGTC 1 cut(s) 398
PvuI CGATCG 1 cut(s) 250
PvuII CAGCTG 1 cut(s) 91
RsaI GTAC 1 cut(s) 282
RsaNI GTAC 1 cut(s) 281
RseI CAYNNNNRTG 2 cut(s) 119, 533
SacI GAGCTC 1 cut(s) 141
SacII CCGCGG 1 cut(s) 330
SaqAI TTAA 4 cut(s) 513, 672, 788, 1083
SatI GCNGC 5 cut(s) 89, 110, 204, 234, 695
Sau3AI GATC 3 cut(s) 247, 574, 930
Sau96I GGNCC 2 cut(s) 502, 987
SchI GAGTC 1 cut(s) 955
ScrFI CCNGG 2 cut(s) 726, 972
SduI GDGCHC 3 cut(s) 141, 186, 597
SfaNI GCATC 3 cut(s) 200, 766, 1044
SfcI CTRYAG 2 cut(s) 316, 580
Sfr303I CCGCGG 1 cut(s) 330
SgrBI CCGCGG 1 cut(s) 330
SinI GGWCC 2 cut(s) 502, 987
SmiMI CAYNNNNRTG 2 cut(s) 119, 533
SphI GCATGC 1 cut(s) 478
Sse9I AATT 4 cut(s) 450, 682, 841, 998
SsiI CCGC 5 cut(s) 327, 329, 565, 585, 695
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 724, 970
StyI CCWWGG 2 cut(s) 469, 598
TaaI ACNGT 2 cut(s) 413, 851
TaqI TCGA 3 cut(s) 246, 250, 732
TasI AATT 4 cut(s) 450, 682, 841, 998
TatI WGTACW 1 cut(s) 280
TauI GCSGC 1 cut(s) 697
TfiI GAWTC 5 cut(s) 223, 252, 568, 678, 1026
Tru1I TTAA 4 cut(s) 513, 672, 788, 1083
Tru9I TTAA 4 cut(s) 513, 672, 788, 1083
TscAI CASTG 3 cut(s) 18, 856, 1036
TseFI GTSAC 2 cut(s) 346, 417
TseI GCWGC 4 cut(s) 88, 109, 203, 233
Tsp45I GTSAC 2 cut(s) 346, 417
TspDTI ATGAA 6 cut(s) 63, 65, 90, 394, 991, 1059
TspGWI ACGGA 1 cut(s) 827
TspRI CASTG 3 cut(s) 18, 856, 1036
Tth111I GACNNNGTC 1 cut(s) 398
Van91I CCANNNNNTGG 1 cut(s) 866
VpaK11BI GGWCC 2 cut(s) 502, 987
XapI RAATTY 2 cut(s) 450, 998
XceI RCATGY 4 cut(s) 478, 701, 740, 754
XcmI CCANNNNNNNNNTGG 1 cut(s) 767
XmnI GAANNNNTTC 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.