Rh1CG310200

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0019788
Physical Location & Seq
Forward (+)
58442695 .. 58443201
507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG310200.1

Sequence Viewer

Length: 507 bp
ATGGCAGCTACGAATCTCAAATTGAATCTCACAATCGACACCGAAAACAACAAGGTTCTATTTGCCATACCGGACAAGGATTTCTTGGATTTCCTTTTCAGCCTTCTGTCTTTGCCTCTTTCAACTGTCACTAGGCTGCTCTCCAGGGACCATAGCGTGCTTGGTAGCTTAGGCAAGCTGCATGAGAGTATCGAAAAGCTTGATCATATGTTCTTCCTGCCTAATCTTAACACTAGGGACACCCTGTTGAAACCCAAATCACCGATCCATCATTTGTTGATGACCAAAGATGACAATGAATCAGCGGCCACAGATGATGGTGGGTATGTTCGGAAACATGCTACATACTTTCTGATGGATAACTTGCAAGTGAAGGCTCCTCTTTCTACGTCTTATATGCTTCCTTTGATTCTGGTTAAGAAACTTGATCCTGAGGATGGTACCGTTAACCAGAGGTGGCTTGAAGTTGAAGAGAGGTGTGTTGATGTGGGCAGGGATGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

168

Amino Acids

19.06

Weight (kDa)

5.46

Isoelectric Point (pI)

27.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 4 - 91 6.1e-18 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0019788)

Species Orthologous Gene IDs
malus_domestica MD11G1084100.v1.1
rosa_multiflora Rmu_sc0002093.1_g000030
rosa_rugosa Rorug01G0323700
rosa_samantha Rh1AG332400 Rh1BG293300 Rh1CG310200 Rh1DG325600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 440
AccB1I GGYRCC 1 cut(s) 440
AciI CCGC 1 cut(s) 305
AclWI GGATC 2 cut(s) 259, 422
AcoI YGGCCR 1 cut(s) 306
AfaI GTAC 1 cut(s) 442
AfiI CCNNNNNNNGG 1 cut(s) 437
AgsI TTSAA 5 cut(s) 25, 123, 250, 464, 470
AjnI CCWGG 1 cut(s) 143
AluBI AGCT 4 cut(s) 8, 168, 178, 199
AluI AGCT 4 cut(s) 8, 168, 178, 199
AlwI GGATC 2 cut(s) 259, 422
AoxI GGCC 1 cut(s) 306
ApeKI GCWGC 3 cut(s) 5, 136, 178
Asp718I GGTACC 1 cut(s) 440
AspS9I GGNCC 1 cut(s) 148
AsuHPI GGTGA 1 cut(s) 252
AvaII GGWCC 1 cut(s) 148
AxyI CCTNAGG 1 cut(s) 432
BanI GGYRCC 1 cut(s) 440
BbvI GCAGC 3 cut(s) 17, 123, 165
BccI CCATC 4 cut(s) 276, 311, 349, 431
BciT130I CCWGG 1 cut(s) 145
BclI TGATCA 1 cut(s) 202
BfaI CTAG 2 cut(s) 132, 234
BisI GCNGC 4 cut(s) 6, 137, 179, 306
BlsI GCNGC 4 cut(s) 7, 138, 180, 307
Bme1390I CCNGG 1 cut(s) 145
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 3 cut(s) 149, 378, 442
BmrFI CCNGG 1 cut(s) 145
BpmI CTGGAG 1 cut(s) 127
Bpu10I CCTNAGC 1 cut(s) 169
BsaJI CCNNGG 1 cut(s) 144
BsaWI WCCGGW 1 cut(s) 70
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse21I CCTNAGG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 145
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 2 cut(s) 442, 502
BseLI CCNNNNNNNGG 1 cut(s) 437
BseMII CTCAG 1 cut(s) 423
BseRI GAGGAG 1 cut(s) 369
BseXI GCAGC 3 cut(s) 17, 123, 165
BshFI GGCC 1 cut(s) 308
BshNI GGYRCC 1 cut(s) 440
BsiSI CCGG 1 cut(s) 71
BslFI GGGAC 2 cut(s) 161, 251
BslI CCNNNNNNNGG 1 cut(s) 437
BsmFI GGGAC 2 cut(s) 161, 251
BsnI GGCC 1 cut(s) 308
Bsp143I GATC 3 cut(s) 202, 264, 427
BspACI CCGC 1 cut(s) 305
BspANI GGCC 1 cut(s) 308
BspCNI CTCAG 1 cut(s) 424
BspLI GGNNCC 3 cut(s) 149, 378, 442
BspPI GGATC 2 cut(s) 259, 422
BspT107I GGYRCC 1 cut(s) 440
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 3 cut(s) 202, 264, 427
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 2 cut(s) 127, 445
Bst6I CTCTTC 1 cut(s) 465
BstC8I GCNNGC 2 cut(s) 158, 176
BstDEI CTNAG 2 cut(s) 169, 432
BstF5I GGATG 2 cut(s) 442, 502
BstKTI GATC 3 cut(s) 205, 267, 430
BstMBI GATC 3 cut(s) 202, 264, 427
BstNI CCWGG 1 cut(s) 145
BstNSI RCATGY 1 cut(s) 341
BstSCI CCNGG 1 cut(s) 143
BstV1I GCAGC 3 cut(s) 17, 123, 165
Bsu36I CCTNAGG 1 cut(s) 432
BsuRI GGCC 1 cut(s) 308
BtsCI GGATG 2 cut(s) 442, 502
Cac8I GCNNGC 2 cut(s) 158, 176
Cfr13I GGNCC 1 cut(s) 148
Csp6I GTAC 1 cut(s) 441
CviAII CATG 2 cut(s) 182, 338
CviJI RGCY 9 cut(s) 8, 102, 136, 168, 178, 199, 308, 377, 460
CviKI_1 RGCY 9 cut(s) 8, 102, 136, 168, 178, 199, 308, 377, 460
CviQI GTAC 1 cut(s) 441
DdeI CTNAG 2 cut(s) 169, 432
DpnI GATC 3 cut(s) 204, 266, 429
DpnII GATC 3 cut(s) 202, 264, 427
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 148
Eco81I CCTNAGG 1 cut(s) 432
EcoRII CCWGG 1 cut(s) 143
FaeI CATG 2 cut(s) 185, 341
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 2 cut(s) 161, 251
FatI CATG 2 cut(s) 181, 337
FauNDI CATATG 1 cut(s) 207
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 4 cut(s) 6, 137, 179, 306
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 4 cut(s) 6, 137, 179, 306
FspBI CTAG 2 cut(s) 132, 234
GluI GCNGC 4 cut(s) 6, 137, 179, 306
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 1 cut(s) 308
HapII CCGG 1 cut(s) 71
Hin1II CATG 2 cut(s) 185, 341
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 4 cut(s) 13, 25, 299, 409
HpaI GTTAAC 1 cut(s) 448
HpaII CCGG 1 cut(s) 71
HphI GGTGA 1 cut(s) 252
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 2 cut(s) 333, 354
Hpy188III TCNNGA 1 cut(s) 431
Hpy8I GTNNAC 1 cut(s) 448
HpyAV CCTTC 2 cut(s) 113, 367
HpyCH4III ACNGT 2 cut(s) 127, 445
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 2 cut(s) 181, 367
HpyF3I CTNAG 2 cut(s) 169, 432
HpySE526I ACGT 1 cut(s) 389
Hsp92II CATG 2 cut(s) 185, 341
KpnI GGTACC 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 202
KspAI GTTAAC 1 cut(s) 448
Kzo9I GATC 3 cut(s) 202, 264, 427
LmnI GCTCC 1 cut(s) 382
LpnPI CCDG 9 cut(s) 84, 130, 157, 230, 257, 398, 444, 464, 478
Lsp1109I GCAGC 3 cut(s) 17, 123, 165
MaeI CTAG 2 cut(s) 132, 234
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 1 cut(s) 127
MalI GATC 3 cut(s) 204, 266, 429
MboI GATC 3 cut(s) 202, 264, 427
MboII GAAGA 2 cut(s) 205, 482
MluCI AATT 1 cut(s) 20
MnlI CCTC 6 cut(s) 126, 390, 427, 447, 468, 493
MseI TTAA 3 cut(s) 228, 417, 447
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 145
MvaI CCWGG 1 cut(s) 145
NdeI CATATG 1 cut(s) 207
NdeII GATC 3 cut(s) 202, 264, 427
NlaIII CATG 2 cut(s) 185, 341
NlaIV GGNNCC 3 cut(s) 149, 378, 442
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 341
PfeI GAWTC 4 cut(s) 13, 25, 299, 409
PkrI GCNGC 4 cut(s) 7, 138, 180, 307
Psp6I CCWGG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 3 cut(s) 149, 378, 442
PspPI GGNCC 1 cut(s) 148
RsaI GTAC 1 cut(s) 442
RsaNI GTAC 1 cut(s) 441
SaqAI TTAA 3 cut(s) 228, 417, 447
SatI GCNGC 4 cut(s) 6, 137, 179, 306
Sau3AI GATC 3 cut(s) 202, 264, 427
Sau96I GGNCC 1 cut(s) 148
ScrFI CCNGG 1 cut(s) 145
SetI ASST 9 cut(s) 10, 57, 170, 180, 201, 392, 458, 479, 504
SinI GGWCC 1 cut(s) 148
Sse9I AATT 1 cut(s) 20
SsiI CCGC 1 cut(s) 305
SspMI CTAG 2 cut(s) 132, 234
StyD4I CCNGG 1 cut(s) 143
TaaI ACNGT 2 cut(s) 127, 445
TaiI ACGT 1 cut(s) 392
TaqI TCGA 2 cut(s) 36, 192
TasI AATT 1 cut(s) 20
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 4 cut(s) 13, 25, 299, 409
Tru1I TTAA 3 cut(s) 228, 417, 447
Tru9I TTAA 3 cut(s) 228, 417, 447
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 3 cut(s) 5, 136, 178
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 148
XceI RCATGY 1 cut(s) 341
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XspI CTAG 2 cut(s) 132, 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.