Rorug02G0041400

Threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0016525
Physical Location & Seq
Forward (+)
3229050 .. 3232283
3234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0041400.1

Sequence Viewer

Length: 1071 bp
ATGGAGAAGAAGAAGGTGGCCGTCCCGCTGGTTTGCCACGGCCATTCCCGGCCGGTCGTGGATCTGTTTTACAGTCCGATCACTCCCGATGGTTTCTTCCTCATCAGTGCCAGTAAGGATTCTAGTCCCATGCTGAGAAATGGAGAGACCGGAGATTGGATTGGAACATTTGAAGGACATAAGGGTGCAGTCTGGAGTTGCTGTTTGGATACTAATGCTCTAAGGGCTGCCACTGCTTCTGCAGATTTCTCTGCGAAAGTTTGGGATGCATTAACAGGAGATGTGCTACACTCGTTTGAACACAAGCACATTGTTCGAGCATGTGCATTCTCAGAGGATACACACCTTTTGCTTACTGGAGGAGTAGAGAAAGTTATACGTATATATGATTTGAACCGCCCAGATGCCCCTCCAAGAGAAGTGGACAAATCCCCTGGTTCGGTTAGAACCGCTGCATGGCTCCACAGTGATCAGACCATTTTAAGTTCTTGCACTGATATGGGGGGTGTCAGATTATGGGATGTAAGAACTGGGAAAATAGTTCAAACACTTGATACCAAGTCATCTGTAACTAGCTCTGAAGTCAGTCAAGATGGTCGTTACATCACAACTGCTGATGGGTCTACAGTGAAGTTTTGGGATGCAAATTACTTTGGATTGGTGAAGAGCTACAACATGCCTTGTACAGTAGAATCAGTTTCGCTGGAACCAAAGTTTGGGAACAAGTTCATTGCTGGTGGTGAAGACATGTGGGTTCATGTTTTTGATTTCCATACCGGTGATGAGATTGCTTGCAATAAGGGTCACCATGGTCCTGTACACTGCGTTCGTTTTTCACCAGGAGGGGAGTCATATGCCTCTGGATCTGAAGATGGCACCATCAGAATATGGCAGACCGGTCCTCTGACTCACGAAGAAAGCGAGGCACAGGCAGCAAATGGGGCAGTCGGAAAGGTGAAGCCACCTGTGGAAGAGGTCTCTCGCAAGATTGAGGGCTTCCAAATTTCTGACGAGGCCAAGTCAAAAGAGAAGGAAGAGGCTCCCGCAGCAGCAGCAGCAGTGAACGTGTGA

Protein Analysis

356

Amino Acids

38.6

Weight (kDa)

5.61

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 9 - 297 9.1e-45 EIF3I
Beta-prop_WDR3_2nd PF25172 12 - 130 2e-07 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 13 - 225 2.8e-28 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 13 - 132 3.5e-13 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 29 - 132 5.5e-11 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 30 - 216 1.5e-13 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 33 - 215 1.2e-20 Prp19 WD40 domain
WD40_Gbeta PF25391 34 - 132 2.3e-06 G protein beta WD-40 repeat protein
WD40 PF00400 54 - 89 5.7e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 77 - 215 5.4e-08 G protein beta WD-40 repeat protein
WDR55 PF24796 99 - 208 1.2e-08 WDR55
Beta-prop_TEP1_2nd PF25047 100 - 216 2.4e-11 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 101 - 214 1.9e-10 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 101 - 238 3.7e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 101 - 300 5e-33 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 105 - 216 1.1e-06 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 157 - 297 1.1e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 190 - 299 6.7e-09 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 190 - 303 1.5e-08 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 201 - 300 4.8e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 230 - 298 5.2e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 230 - 314 1.9e-11 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 231 - 300 7.7e-12 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 242 - 299 4.4e-10 WDR5 beta-propeller domain
WD40 PF00400 266 - 297 6.2e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29840
fragaria_vesca FvH4_1g08110 FvH4_4g03760
malus_domestica MD15G1212700.v1.1
pyrus_communis pycom15g18900
rosa_chinensis RchiOBHm_Chr2g0094161
rosa_laevigata RLG00000016461
rosa_multiflora Rmu_co8387495.1_g000001
rosa_roxburghii Rroxscaffold_2G00147430
rosa_rugosa Rorug02G0041400
rosa_samantha Rh2AG089100 Rh2BG089200 Rh2CG091700 Rh2DG088200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 875
AccB7I CCANNNNNTGG 1 cut(s) 716
AccI GTMKAC 1 cut(s) 623
AciI CCGC 4 cut(s) 26, 397, 450, 1044
AclWI GGATC 2 cut(s) 69, 871
AcoI YGGCCR 3 cut(s) 18, 40, 50
AcsI RAATTY 1 cut(s) 1002
AcuI CTGAAG 2 cut(s) 600, 888
AfaI GTAC 2 cut(s) 685, 819
AfiI CCNNNNNNNGG 4 cut(s) 156, 439, 456, 716
AflIII ACRYGT 2 cut(s) 747, 1065
AgeI ACCGGT 2 cut(s) 776, 896
AgsI TTSAA 4 cut(s) 173, 299, 394, 545
AjnI CCWGG 2 cut(s) 433, 838
AjuI GAANNNNNNNTTGG 2 cut(s) 699, 731
AluBI AGCT 2 cut(s) 576, 669
AluI AGCT 2 cut(s) 576, 669
Alw26I GTCTC 2 cut(s) 140, 982
AlwI GGATC 2 cut(s) 69, 871
AoxI GGCC 4 cut(s) 18, 40, 50, 1014
ApeKI GCWGC 7 cut(s) 227, 452, 932, 1046, 1049, 1052, 1055
ApoI RAATTY 1 cut(s) 1002
AsiGI ACCGGT 2 cut(s) 776, 896
Asp700I GAANNNNTTC 1 cut(s) 725
AspS9I GGNCC 2 cut(s) 812, 899
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 6 cut(s) 673, 752, 791, 797, 828, 967
AvaII GGWCC 2 cut(s) 812, 899
BanI GGYRCC 1 cut(s) 875
BbsI GAAGAC 1 cut(s) 750
BbvI GCAGC 7 cut(s) 214, 439, 944, 1058, 1061, 1064, 1067
BccI CCATC 5 cut(s) 83, 587, 611, 866, 887
BceAI ACGGC 2 cut(s) 5, 55
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 2 cut(s) 435, 840
BciVI GTATCC 2 cut(s) 202, 331
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 140, 982
BfaI CTAG 2 cut(s) 123, 573
BfmI CTRYAG 2 cut(s) 240, 624
BfuI GTATCC 2 cut(s) 202, 331
BisI GCNGC 7 cut(s) 228, 453, 933, 1047, 1050, 1053, 1056
BlsI GCNGC 7 cut(s) 229, 454, 934, 1048, 1051, 1054, 1057
Bme1390I CCNGG 3 cut(s) 49, 435, 840
Bme18I GGWCC 2 cut(s) 812, 899
BmgT120I GGNCC 2 cut(s) 812, 899
BmiI GGNNCC 4 cut(s) 461, 708, 877, 1041
BmrFI CCNGG 3 cut(s) 49, 435, 840
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 3 cut(s) 256, 394, 631
BmuI ACTGGG 1 cut(s) 540
BpiI GAAGAC 1 cut(s) 750
BpmI CTGGAG 2 cut(s) 214, 378
BpuMI CCSGG 1 cut(s) 49
BsaAI YACGTR 1 cut(s) 380
BsaI GGTCTC 2 cut(s) 140, 982
BsaJI CCNNGG 3 cut(s) 37, 433, 808
BsaWI WCCGGW 3 cut(s) 149, 776, 896
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 439, 456, 716
Bse118I RCCGGY 3 cut(s) 52, 776, 896
Bse1I ACTGG 3 cut(s) 111, 361, 535
Bse3DI GCAATG 1 cut(s) 729
BseBI CCWGG 2 cut(s) 435, 840
BseDI CCNNGG 3 cut(s) 37, 433, 808
BseGI GGATG 3 cut(s) 271, 526, 646
BseLI CCNNNNNNNGG 4 cut(s) 156, 439, 456, 716
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 2 cut(s) 125, 345
BseNI ACTGG 3 cut(s) 111, 361, 535
BseRI GAGGAG 1 cut(s) 375
BseX3I CGGCCG 1 cut(s) 50
BseXI GCAGC 7 cut(s) 214, 439, 944, 1058, 1061, 1064, 1067
BsgI GTGCAG 1 cut(s) 207
Bsh1285I CGRYCG 2 cut(s) 53, 57
BshFI GGCC 4 cut(s) 20, 42, 52, 1016
BshNI GGYRCC 1 cut(s) 875
BshTI ACCGGT 2 cut(s) 776, 896
BsiEI CGRYCG 2 cut(s) 53, 57
BsiSI CCGG 5 cut(s) 49, 53, 150, 777, 897
BslFI GGGAC 2 cut(s) 8, 111
BslI CCNNNNNNNGG 4 cut(s) 156, 439, 456, 716
BsmAI GTCTC 2 cut(s) 140, 982
BsmFI GGGAC 2 cut(s) 8, 111
BsmI GAATGC 1 cut(s) 326
BsnI GGCC 4 cut(s) 20, 42, 52, 1016
Bso31I GGTCTC 2 cut(s) 140, 982
Bsp1407I TGTACA 2 cut(s) 683, 817
Bsp143I GATC 4 cut(s) 61, 78, 469, 863
Bsp19I CCATGG 1 cut(s) 808
BspACI CCGC 4 cut(s) 26, 397, 450, 1044
BspANI GGCC 4 cut(s) 20, 42, 52, 1016
BspCNI CTCAG 2 cut(s) 126, 344
BspLI GGNNCC 4 cut(s) 461, 708, 877, 1041
BspMAI CTGCAG 1 cut(s) 244
BspPI GGATC 2 cut(s) 69, 871
BspQI GCTCTTC 1 cut(s) 659
BspT107I GGYRCC 1 cut(s) 875
BspTNI GGTCTC 2 cut(s) 140, 982
BsrDI GCAATG 1 cut(s) 729
BsrFI RCCGGY 3 cut(s) 52, 776, 896
BsrGI TGTACA 2 cut(s) 683, 817
BsrI ACTGG 3 cut(s) 111, 361, 535
BssAI RCCGGY 3 cut(s) 52, 776, 896
BssECI CCNNGG 3 cut(s) 37, 433, 808
BssMI GATC 4 cut(s) 61, 78, 469, 863
BssT1I CCWWGG 1 cut(s) 808
Bst2UI CCWGG 2 cut(s) 435, 840
Bst4CI ACNGT 4 cut(s) 74, 467, 628, 688
Bst6I CTCTTC 3 cut(s) 659, 966, 1029
BstAUI TGTACA 2 cut(s) 683, 817
BstBAI YACGTR 1 cut(s) 380
BstC8I GCNNGC 1 cut(s) 793
BstDEI CTNAG 3 cut(s) 134, 221, 331
BstDSI CCRYGG 2 cut(s) 37, 808
BstEII GGTNACC 1 cut(s) 803
BstF5I GGATG 3 cut(s) 271, 526, 646
BstKTI GATC 4 cut(s) 64, 81, 472, 866
BstMAI GTCTC 2 cut(s) 140, 982
BstMBI GATC 4 cut(s) 61, 78, 469, 863
BstMCI CGRYCG 2 cut(s) 53, 57
BstMWI GCNNNNNNNGC 7 cut(s) 224, 233, 932, 941, 1046, 1052, 1055
BstNI CCWGG 2 cut(s) 435, 840
BstNSI RCATGY 3 cut(s) 324, 679, 751
BstPI GGTNACC 1 cut(s) 803
BstSCI CCNGG 3 cut(s) 47, 433, 838
BstSFI CTRYAG 2 cut(s) 240, 624
BstSNI TACGTA 1 cut(s) 380
BstV1I GCAGC 7 cut(s) 214, 439, 944, 1058, 1061, 1064, 1067
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 2 cut(s) 61, 863
BstYI RGATCY 2 cut(s) 61, 863
BstZI CGGCCG 1 cut(s) 50
BsuI GTATCC 2 cut(s) 202, 331
BsuRI GGCC 4 cut(s) 20, 42, 52, 1016
BtgI CCRYGG 2 cut(s) 37, 808
BtsCI GGATG 3 cut(s) 271, 526, 646
BtsI GCAGTG 3 cut(s) 231, 820, 1065
BtsIMutI CAGTG 7 cut(s) 112, 231, 472, 492, 633, 820, 1065
Cac8I GCNNGC 1 cut(s) 793
Cfr10I RCCGGY 3 cut(s) 52, 776, 896
Cfr13I GGNCC 2 cut(s) 812, 899
Csp6I GTAC 2 cut(s) 684, 818
CspAI ACCGGT 2 cut(s) 776, 896
CspCI CAANNNNNGTGG 2 cut(s) 402, 437
CviAII CATG 7 cut(s) 130, 321, 456, 676, 748, 758, 809
CviQI GTAC 2 cut(s) 684, 818
DdeI CTNAG 3 cut(s) 134, 221, 331
DpnI GATC 4 cut(s) 63, 80, 471, 865
DpnII GATC 4 cut(s) 61, 78, 469, 863
EaeI YGGCCR 3 cut(s) 18, 40, 50
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 3 cut(s) 659, 966, 1029
EarI CTCTTC 3 cut(s) 659, 966, 1029
EclXI CGGCCG 1 cut(s) 50
Eco105I TACGTA 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 808
Eco31I GGTCTC 2 cut(s) 140, 982
Eco47I GGWCC 2 cut(s) 812, 899
Eco52I CGGCCG 1 cut(s) 50
Eco57I CTGAAG 2 cut(s) 600, 888
Eco91I GGTNACC 1 cut(s) 803
EcoO65I GGTNACC 1 cut(s) 803
EcoRII CCWGG 2 cut(s) 433, 838
EcoT14I CCWWGG 1 cut(s) 808
EcoT22I ATGCAT 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 808
FaeI CATG 7 cut(s) 133, 324, 459, 679, 751, 761, 812
FaqI GGGAC 2 cut(s) 8, 111
FatI CATG 7 cut(s) 129, 320, 455, 675, 747, 757, 808
FauI CCCGC 2 cut(s) 33, 1051
FauNDI CATATG 1 cut(s) 853
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 7 cut(s) 228, 453, 933, 1047, 1050, 1053, 1056
FokI GGATG 3 cut(s) 278, 533, 653
Fsp4HI GCNGC 7 cut(s) 228, 453, 933, 1047, 1050, 1053, 1056
FspBI CTAG 2 cut(s) 123, 573
GluI GCNGC 7 cut(s) 228, 453, 933, 1047, 1050, 1053, 1056
GsuI CTGGAG 2 cut(s) 214, 378
HaeIII GGCC 4 cut(s) 20, 42, 52, 1016
HapII CCGG 5 cut(s) 49, 53, 150, 777, 897
Hin1II CATG 7 cut(s) 133, 324, 459, 679, 751, 761, 812
HinfI GANTC 4 cut(s) 119, 692, 848, 907
HpaII CCGG 5 cut(s) 49, 53, 150, 777, 897
HphI GGTGA 6 cut(s) 673, 752, 791, 797, 828, 967
Hpy166II GTNNAC 4 cut(s) 424, 624, 820, 1063
Hpy188III TCNNGA 5 cut(s) 86, 193, 590, 861, 911
Hpy8I GTNNAC 4 cut(s) 424, 624, 820, 1063
HpyAV CCTTC 3 cut(s) 7, 167, 1024
HpyCH4III ACNGT 4 cut(s) 74, 467, 628, 688
HpyCH4IV ACGT 2 cut(s) 379, 1065
HpyCH4V TGCA 8 cut(s) 188, 242, 269, 326, 455, 492, 644, 795
HpyF10VI GCNNNNNNNGC 7 cut(s) 224, 233, 932, 941, 1046, 1052, 1055
HpyF3I CTNAG 3 cut(s) 134, 221, 331
HpySE526I ACGT 2 cut(s) 379, 1065
Hsp92II CATG 7 cut(s) 133, 324, 459, 679, 751, 761, 812
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 4 cut(s) 61, 78, 469, 863
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 2 cut(s) 465, 1045
Lsp1109I GCAGC 7 cut(s) 214, 439, 944, 1058, 1061, 1064, 1067
LweI GCATC 3 cut(s) 256, 394, 631
MaeI CTAG 2 cut(s) 123, 573
MaeII ACGT 2 cut(s) 379, 1065
MaeIII GTNAC 3 cut(s) 568, 599, 803
MalI GATC 4 cut(s) 63, 80, 471, 865
MboI GATC 4 cut(s) 61, 78, 469, 863
MboII GAAGA 9 cut(s) 19, 22, 88, 676, 755, 881, 926, 983, 1046
MflI RGATCY 2 cut(s) 61, 863
MluCI AATT 2 cut(s) 646, 1002
MlyI GAGTC 2 cut(s) 857, 901
MmeI TCCRAC 1 cut(s) 928
Mph1103I ATGCAT 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 725
MseI TTAA 2 cut(s) 272, 482
MslI CAYNNNNRTG 3 cut(s) 183, 497, 777
MspA1I CMGCKG 2 cut(s) 28, 452
MspI CCGG 5 cut(s) 49, 53, 150, 777, 897
MspR9I CCNGG 3 cut(s) 49, 435, 840
Mva1269I GAATGC 1 cut(s) 326
MvaI CCWGG 2 cut(s) 435, 840
MwoI GCNNNNNNNGC 7 cut(s) 224, 233, 932, 941, 1046, 1052, 1055
NciI CCSGG 1 cut(s) 49
NcoI CCATGG 1 cut(s) 808
NdeI CATATG 1 cut(s) 853
NdeII GATC 4 cut(s) 61, 78, 469, 863
NlaIII CATG 7 cut(s) 133, 324, 459, 679, 751, 761, 812
NlaIV GGNNCC 4 cut(s) 461, 708, 877, 1041
NmuCI GTSAC 1 cut(s) 803
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 3 cut(s) 324, 679, 751
PciI ACATGT 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 659
PcsI WCGNNNNNNNCGW 1 cut(s) 918
PctI GAATGC 1 cut(s) 326
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 2 cut(s) 119, 692
PflMI CCANNNNNTGG 1 cut(s) 716
PinAI ACCGGT 2 cut(s) 776, 896
PkrI GCNGC 7 cut(s) 229, 454, 934, 1048, 1051, 1054, 1057
PleI GAGTC 2 cut(s) 856, 901
PpsI GAGTC 2 cut(s) 856, 901
Ppu21I YACGTR 1 cut(s) 380
PscI ACATGT 1 cut(s) 747
Psp6I CCWGG 2 cut(s) 433, 838
PspEI GGTNACC 1 cut(s) 803
PspGI CCWGG 2 cut(s) 433, 838
PspN4I GGNNCC 4 cut(s) 461, 708, 877, 1041
PspPI GGNCC 2 cut(s) 812, 899
PsrI GAACNNNNNNTAC 2 cut(s) 810, 842
PstI CTGCAG 1 cut(s) 244
PsuI RGATCY 2 cut(s) 61, 863
RsaI GTAC 2 cut(s) 685, 819
RsaNI GTAC 2 cut(s) 684, 818
RseI CAYNNNNRTG 3 cut(s) 183, 497, 777
SapI GCTCTTC 1 cut(s) 659
SaqAI TTAA 2 cut(s) 272, 482
SatI GCNGC 7 cut(s) 228, 453, 933, 1047, 1050, 1053, 1056
Sau3AI GATC 4 cut(s) 61, 78, 469, 863
Sau96I GGNCC 2 cut(s) 812, 899
SchI GAGTC 2 cut(s) 857, 901
ScrFI CCNGG 3 cut(s) 49, 435, 840
SetI ASST 9 cut(s) 18, 348, 382, 578, 671, 957, 967, 978, 1068
SfaNI GCATC 3 cut(s) 256, 394, 631
SfcI CTRYAG 2 cut(s) 240, 624
SinI GGWCC 2 cut(s) 812, 899
SmiMI CAYNNNNRTG 3 cut(s) 183, 497, 777
SnaBI TACGTA 1 cut(s) 380
Sse9I AATT 2 cut(s) 646, 1002
SsiI CCGC 4 cut(s) 26, 397, 450, 1044
SspMI CTAG 2 cut(s) 123, 573
StyD4I CCNGG 3 cut(s) 47, 433, 838
StyI CCWWGG 1 cut(s) 808
TaaI ACNGT 4 cut(s) 74, 467, 628, 688
TaiI ACGT 2 cut(s) 382, 1068
TaqI TCGA 1 cut(s) 316
TasI AATT 2 cut(s) 646, 1002
TatI WGTACW 2 cut(s) 683, 817
TfiI GAWTC 2 cut(s) 119, 692
Tru1I TTAA 2 cut(s) 272, 482
Tru9I TTAA 2 cut(s) 272, 482
TscAI CASTG 7 cut(s) 112, 238, 472, 499, 633, 827, 1065
TseFI GTSAC 1 cut(s) 803
TseI GCWGC 7 cut(s) 227, 452, 932, 1046, 1049, 1052, 1055
Tsp45I GTSAC 1 cut(s) 803
TspDTI ATGAA 2 cut(s) 718, 746
TspRI CASTG 7 cut(s) 112, 238, 472, 499, 633, 827, 1065
Van91I CCANNNNNTGG 1 cut(s) 716
VpaK11BI GGWCC 2 cut(s) 812, 899
XapI RAATTY 1 cut(s) 1002
XceI RCATGY 3 cut(s) 324, 679, 751
XmiI GTMKAC 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 2 cut(s) 123, 573
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.